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pGEX-SP1

Expression vector of human SP1 in E. coli

Catalog number RDB05699
Resource name pGEX-SP1
Clone info. Expression vector of human SP1 in E. coli.
Vector backbone pGEX-2TK (Plasmid)
Size of vector backbone 4.97 kb
Selectable markers Amp^r
Gene/insert name Human Sp1 transcription factor cDNA
Depositor|Developer Yokoyama, Kazunari | Song, Jun |
Other clones in our bank

External Database
Human Sp1 transcription factor

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR Manuscripts for the publication shall be made through the discussion between Depositor and Recipient Investigator. Recipient investigator shall include Depositor/Developer as co-author in manuscript for the publication. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (J. Biol. Chem. 276, 19897-19904, 2001). )Recipient Investigator shall negotiate with Developer/Depositor when patent application and the commercialization for the products based upon Material, derivatives thereof their use is considered.
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
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          提供条件 使用にあたっては常に密な連絡をとり、発表等に関しても 事前に合意を要する。共著とする。利用者は、研究成果の公表にあたって寄託者の指 定する文献を引用する(J. Biol. Chem. 276, 19897-19904, 2001). 。本リソースを 使用して商業利用する場合は、寄託者と改めて契約する。
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          RDB05699 pGEX-SP1 DNA solution

          Ordering Information [in Japanese] [in English]
          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          Further references such as user reports and related articles (go to bottom)

          Featured content


          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB12833_A4Gp.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: pGEX-F2
          Region: GST,insert 5'(SP1)
          Sequence file: RDB12833_A4Gpa.seq check
          >05699_12833_B1DrS_pGEX-F2_D02_11_ABI24.ab1
              1 CNNNNNNNTN NNNAANTACT TNAAATCCAG CAAGTATATA GCATGGCCTT TGCAGGGCTG
             61 GCAAGCCACG TTTGGTGGTG GCGACCATCC TCCAAAATCG GATCTGGTTC CGCGTGGATC
            121 TCGTCGTGCA TCTGTTGGAT CGATCGCGAG GCCTCGAGCC ATGGATCCCC CCATGGATGA
            181 AATGACAGCT GTGGTGAAAA TTGAAAAAGG AGTTGGTGGC AATAATGGGG GCAATGGTAA
            241 TGGTGGTGGT GCCTTTTCAC AGGCTCGAAG TAGCAGCACA GGCAGTAGCA GCAGCACTGG
            301 AGGAGGAGGG CAGGAGTCCC AGCCATCCCC TTTGGCTCTG CTGGCAGCAA CTTGCAGCAG
            361 AATTGAGTCA CCCAATGAGA ACAGCAACAA CTCCCAGGGC CCGAGTCAGT CAGGGGGAAC
            421 AGGTGAGCTT GACCTCACAG CCACACAACT TTCACAGGGT GCCAATGGCT GGCAGATCAT
            481 CTCTTCCTCC TCTGGGGCTA CCCCTACCTC AAAGGAACAG AGTGGCAGCA GTACCAATGG
            541 CAGCAATGGC AGTGAGTCTT CCNAGAATCG CACAGTCTCT GGTGGGCAGT ATGTTGTGGC
            601 TGCCGCTCCN NACTTACAGA ACCNGCAAGT TCTGANAGGN NTANCNNNNN NGANGNNNNN
          661 NANNNNGNAT CNNNTNNTNN NNNNNNTNNN NNANNNNTNN NNGGNNNNNN NNNN
          //
          Primer: pJB8-Forward
          Region: Vector junction
          Sequence file: RDB12833_A4Gpb.seq check
          >05699_12833_B1DrS_pJB8-Forward_E02_14_ABI24.ab1
              1 NNNNNNNNNN NGCGCCCCGA CACCCGCCNA CACCCGCTGA CGCGCCCTGA CGGGCTTGTC
             61 TGCTCCCGGC ATCCGCTTAC AGACAAGCTG TGACCGTCTC CGGGAGCTGC ATGTGTCAGA
            121 GGTTTTCACC GTCATCACCG AAACGCGCGA GGCAGATCGT CAGTCAGTCA CGATGAATTC
            181 CGGTGCAGGT CTCTTTTAAT ATTGGCTAGC AGAAAACCTA TCCCCAACCT AACAGATTAC
            241 ACACACACAC ACACACACAC ACACACAGAG CTATTTTTCC ACACTTCCTC ACAGTGAAAA
            301 GCTCTCTAAC AAGATACCAG AAGGTCGAGA GAGACAATGC TTTTATGGCT GGGCCTTGAG
            361 GACAATTCTG GTTGCATTTA AATCTCAGTG CCAAATGCAC TAGGTTCCCT GCCGTTATAA
            421 TTTTTGGATC AGTCTCTTCT GAAATCAGGT CACAGAGAAA ACAGGATGAT TTCTCCCAAT
            481 CTCTCCTATA AAACACTGTG GTAAGACAAG ATTTCTTTTT GTTCTGGGCT ATCATTCCCA
            541 TGATCATGTG CTTCCCTTTC CCTTANGAAC CACCCAAGAA CATACAAGGA AAAGTTAANG
            601 GANNACATCA TAAGAACCAC TGTCTTGNCA CTAGCACATG CTTTGNNNNA NTTCTGCANA
          661 ATNNNANNNN NNAANNGANT CNNNGNNNNN NNNNN
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          user report Membrino, A., G4-DNA Formation in the HRAS Promoter and Rational Design of Decoy Oligonucleotides for Cancer Therapy. PLoS One. 6 (9): e24421 (2011). PMID 21931711.

          2022.02.09

          GNP_filter3_RDBDEP_html_220112.pl