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pGEX-SP1

Expression vector of human SP1 in E. coli

Catalog number RDB05699
Resource name pGEX-SP1
Clone info. Expression vector of human SP1 in E. coli.
Vector backbone pGEX-2TK (Plasmid)
Size of vector backbone 4.97 kb
Selectable markers Amp^r
Gene/insert name Human Sp1 transcription factor cDNA
Depositor Yokoyama, Kazunari | Song, Jun |
 
Other clones in our bank human SP1 (NCBI Gene 6667) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR Manuscripts for the publication shall be made through the discussion between Depositor and Recipient Investigator. Recipient investigator shall include Depositor/Developer as co-author in manuscript for the publication. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (J. Biol. Chem. 276, 19897-19904, 2001). )Recipient Investigator shall negotiate with Developer/Depositor when patent application and the commercialization for the products based upon Material, derivatives thereof their use is considered.
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB05699 pGEX-SP1 DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Featured content

References

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB12833_A4Gp.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pGEX_F RDB12833_A4Gpa.seq
>05699_12833_A4Gp_pGEX_F_G01_19.ab1
    1 AGCCCCCCCA AATCGGGAAT CTGGTTCCGC GTGGATCTCG TCGTGCATCT GTTGGATCGA
   61 TCGCGAGGCC TCGAGCCATG GATCCCCCCA TGGATGAAAT GACAGCTGTG GTGAAAATTG
  121 AAAAAGGAGT TGGTGGCAAT AATGGGGGCA ATGGTAATGG TGGTGGTGCC TTTTCACAGG
  181 CTCGAAGTAG CAGCACAGGC AGTAGCAGCA GCACTGGAGG AGGAGGGCAG GAGTCCCAGC
  241 CATCCCCTTT GGCTCTGCTG GCAGCAACTT GCAGCAGAAT TGAGTCACCC AATGAGAACA
  301 GCAACAACTC CCAGGGCCCG AGTCAGTCAG GGGGAACAGG TGAGCTTGAC CTCACAGCCA
  361 CACAACTTTC ACAGGGTGCC AATGGCTGGC AGATCATCTC TTCCTCCTCT GGGGCTACCC
  421 CTACCTCAAA GGAACAGAGT GGCAGCAGTA CCAATGGCAG CAATGGCAGT GAGTCTTCCA
  481 AGAATCGCAC AGTCTCTGGT GGGCAGTATG TTGTGGCTGC CGCTCCCAAC TTACAGAACC
  541 AGCAAGTTCT GACAGGACTA CCTGGAGTGA TGCCTAATAT TCAGTATCAA GTAATCCCAC
  601 AGTTCCAGAC CGTTGATGGG CAACAGCTGC AGTTTGCTGC CACTGGGGCC CAAGTGCAGC
  661 AGGATGGTTC TGGTCAAATA CAGATCATAC CAGGTGCAAA CCAACAGATT ATCACAAATC
  721 GAGGAAGTGG AGGCAACATC ATTGCTGCTA TGCCAAACCT ACTCCAGCAG GCTGTCCCCC
  781 TCCAAGGCCT GGCTAATAAT GTACTCTCAG GACAGACTCA GTATGTGACC AATGTACCAG
  841 TGGCCCTGAA TGGGAACATC ACCTTGCTAC CTGTCAACAG CGTTTCTGCA GCTACCTTGA
  901 CTCCCAGCTC TCAGCAGTCA CGATCAGCAG CTCTGGGTCC CAGGAGAGTG GCTCACAGCC
  961 TGTCACCTCA GGACTACCAT CAGTTCTGCC AGCTTGGTAT CATCACAAGC CAGTTCCAGC
 1021 TCCTTTTTCA CCATGCCATA GCTACTCACT ACTACTACAC AGCCAACATG GGATTATGGA
 1081 CCTTACCTAC AGTGATCAAT CAGCACCACT CTCAAGGCCA GACCCCCAGA AGGTTCAGTG
1141 GGCCTTCAAG GGGTCTCTGA AT
//
Primer: pGEX_R RDB12833_A4Gpb.seq
>05699_12833_A4Gp_pGEX_R_H01_22.ab1
    1 GCCCGTACGG AACGCGCGAG GCAGATCGTC CCTCTCTCAC GATGAATTCC GGTGCAGGTC
   61 GCTTTTACTA TTGGCTAGCA GAAAACCTAT CCCCAAACTA ACAGATTACA CACACACACA
  121 CACACACACA CACGCAGAGC TATTTTTCCA CACTTCCTCA CAGTGAAAAG CTCTCTAACA
  181 AGATACCAGA AGGTCGAGAG AGACAATGCT TTTATGGCTG GGCCTTGAGG ACAATTCTGG
  241 TTGCATTTAA ATCTCAATGC CAAATGCACT AGGTTCCCTG CCGTTATAAT TTTTGGATCA
  301 GTCTCTTCTG AAATCAGGTC ACAGAGAAAA CAGGATGATT TCTCCCAATC TCTCCTATAA
  361 AACACTGTGG TAAGACAAGA TTTCTTTTTG TTCTGGGCTA TCATTCCCAT GATCATGTGC
  421 TTCCCTTTCC CTTAGGAACC ACCCAAGAAC ATACAAGGAA AAGTTAAGGG AAAACATCAT
  481 AAGAACCACT GTCTTGCCAC TAGCACATGC TTTGGAGGAG TTCTGCAGAA TGGAAACCTG
  541 AAACAGATTC ACTGGCTGAT GCTCCTTATT GACAATGGAA AAGAACCTAG TGGGGATGTT
  601 CAAAAGAAGT GTATTTCCTT TCAGAATATA AACTCAGAAG CCCACACATC AAAACTTAGA
  661 GCTACCTTGG AAGACAGCAG TACCAAAGTC CAAATACTCC ACTAACTCCT GAACCTGACT
  721 ACGAGTCATT TCCTCTTCTG AGCACATGAT TTTCCTAGTG AAAGCCCCCT ACCTATTATT
  781 TGAAAATTTG ACAGTTTATA CAAACTGAGC CAAGATTATT ATCTTCAAAG GGACTCTTGT
  841 ACAATTCCAC TGAAAGACTT CTAATTCTTG ATGCTCAGAG CAGCTAATGA AGGGAGATGC
  901 CTTTGACTCT CCAGCGCCCA AGAAAGTGCC TGCGTCAGTA GTATTTTGAA CACTCCCTCC
  961 ACAGTTACTT CAAGCATCAG CCTGGCTTCA AGGATTTTTC CATTCTGCTG CTGGAACTAC
 1021 GTTCTGAAAA ATTGACAGAA GTGCCTCATC CAGGCAGTAC AAAACAACAT GAGTGATCAG
 1081 ATGAGAAGAG GATGAACGGC CAACAGTGAG TAGGGCAGGA TGCCAGAGCC TGATTCGACC
1141 AAAGACAAGG TGAGAAAGGC AAAACAGTTA GC
//

Please visit Sequencing and PCR primers for primer information.


References

References

user report Membrino, A., G4-DNA Formation in the HRAS Promoter and Rational Design of Decoy Oligonucleotides for Cancer Therapy. PLoS One. 6 (9): e24421 (2011). PMID 21931711.

2018.12.11

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