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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pGEX-SP1 (#RDB05699)

Expression vector of human SP1 in E. coli

Clone info. Expression vector of human SP1 in E. coli.
Vector backbone pGEX-2TK (Plasmid)
Size of vector backbone 4.97 kb
Selectable markers Amp^r
Gene/insert name Human Sp1 transcription factor cDNA
Depositor|Developer Yokoyama, Kazunari | Song, Jun |
 

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Terms and conditions for distribution Manuscripts for the publication shall be made through the discussion between Depositor and Recipient Investigator. Recipient investigator shall include Depositor/Developer as co-author in manuscript for the publication. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (J. Biol. Chem. 276, 19897-19904, 2001). )Recipient Investigator shall negotiate with Developer/Depositor when patent application and the commercialization for the products based upon Material, derivatives thereof their use is considered.
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB05699 pGEX-SP1 DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pGEX-SP1 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB05699).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB12833_A4Gpp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pGEX-F2 (Pr0403)
Region: GST,insert 5'(SP1)
Sequence file: RDB12833_A4Gpa.seq check
>05699_12833_B1DrS_pGEX-F2_D02_11_ABI24.ab1
    1 CNNNNNNNTN NNNAANTACT TNAAATCCAG CAAGTATATA GCATGGCCTT TGCAGGGCTG
   61 GCAAGCCACG TTTGGTGGTG GCGACCATCC TCCAAAATCG GATCTGGTTC CGCGTGGATC
  121 TCGTCGTGCA TCTGTTGGAT CGATCGCGAG GCCTCGAGCC ATGGATCCCC CCATGGATGA
  181 AATGACAGCT GTGGTGAAAA TTGAAAAAGG AGTTGGTGGC AATAATGGGG GCAATGGTAA
  241 TGGTGGTGGT GCCTTTTCAC AGGCTCGAAG TAGCAGCACA GGCAGTAGCA GCAGCACTGG
  301 AGGAGGAGGG CAGGAGTCCC AGCCATCCCC TTTGGCTCTG CTGGCAGCAA CTTGCAGCAG
  361 AATTGAGTCA CCCAATGAGA ACAGCAACAA CTCCCAGGGC CCGAGTCAGT CAGGGGGAAC
  421 AGGTGAGCTT GACCTCACAG CCACACAACT TTCACAGGGT GCCAATGGCT GGCAGATCAT
  481 CTCTTCCTCC TCTGGGGCTA CCCCTACCTC AAAGGAACAG AGTGGCAGCA GTACCAATGG
  541 CAGCAATGGC AGTGAGTCTT CCNAGAATCG CACAGTCTCT GGTGGGCAGT ATGTTGTGGC
  601 TGCCGCTCCN NACTTACAGA ACCNGCAAGT TCTGANAGGN NTANCNNNNN NGANGNNNNN
661 NANNNNGNAT CNNNTNNTNN NNNNNNTNNN NNANNNNTNN NNGGNNNNNN NNNN
//
Primer: pJB8-Forward (Pr0084)
Region: Vector junction
Sequence file: RDB12833_A4Gpb.seq check
>05699_12833_B1DrS_pJB8-Forward_E02_14_ABI24.ab1
    1 NNNNNNNNNN NGCGCCCCGA CACCCGCCNA CACCCGCTGA CGCGCCCTGA CGGGCTTGTC
   61 TGCTCCCGGC ATCCGCTTAC AGACAAGCTG TGACCGTCTC CGGGAGCTGC ATGTGTCAGA
  121 GGTTTTCACC GTCATCACCG AAACGCGCGA GGCAGATCGT CAGTCAGTCA CGATGAATTC
  181 CGGTGCAGGT CTCTTTTAAT ATTGGCTAGC AGAAAACCTA TCCCCAACCT AACAGATTAC
  241 ACACACACAC ACACACACAC ACACACAGAG CTATTTTTCC ACACTTCCTC ACAGTGAAAA
  301 GCTCTCTAAC AAGATACCAG AAGGTCGAGA GAGACAATGC TTTTATGGCT GGGCCTTGAG
  361 GACAATTCTG GTTGCATTTA AATCTCAGTG CCAAATGCAC TAGGTTCCCT GCCGTTATAA
  421 TTTTTGGATC AGTCTCTTCT GAAATCAGGT CACAGAGAAA ACAGGATGAT TTCTCCCAAT
  481 CTCTCCTATA AAACACTGTG GTAAGACAAG ATTTCTTTTT GTTCTGGGCT ATCATTCCCA
  541 TGATCATGTG CTTCCCTTTC CCTTANGAAC CACCCAAGAA CATACAAGGA AAAGTTAANG
  601 GANNACATCA TAAGAACCAC TGTCTTGNCA CTAGCACATG CTTTGNNNNA NTTCTGCANA
661 ATNNNANNNN NNAANNGANT CNNNGNNNNN NNNNN
//
Primer: hSP1_rev (Pr0999)
Region: insert 3'
Sequence file: RDB12833_A4Gpc.seq check
>05699_12833_B1DrS_hSP1_rev_G01_19_ABI24.ab1
    1 NNNNNNNNNN NNNNTTTNAT GCTTNNTGGC TCTCTCTTCN NTGTCTCTTG GACCCATGCT
   61 ACCTTGCATC CCGGGGTTAA GGGGTATGGC CCATATGTCT CTGGCCCCGG GTGCCTGATC
  121 TCAGAAGCCA TTGCCACTGA TATTAATGGA CTGCAGATCT GCCACCTGCA TGACGTTGAT
  181 GCCACTGTTG GCAAGACGGG CAATGCCCTC TGGACAGATG GCCTCCATGG CTACCATATT
  241 GGTGGTAATA AGGGCTGAAG GAGTGGCAGT GCCACTGCCT TCTGAACCTG CCCCACTGTC
  301 CAGGGGCAAA GTGCCCACAC TCAGAGCTAC ACCTGGGCCT CCCTTCTTAT TCTGGTGGGT
  361 CTTGATATGT TTTGACAGGT GGTCACTCCT CATGAAGCGC TTAGGACACT CAGGGCAGGC
  421 AAATTTCTTC TCACCTGTGT GTGTACGTTT GTGCCTCTGT AGCTCATCCG AACGTGTGAA
  481 GCGTTTCCCA CAGTATGACC AGGTACACAT AAATGGCCTC TCGCCTGTAT GCCAGCGCAA
  541 GTGTGCCCGC AGGTGAGAGG TCTTGCCATA CACTTTCCCA CAGCCTTGGA TGTGGCAAAT
  601 ATGCTGTTTC TTNNTGCCNG GATCCCCCGA GCCCCTTCCT TCNCTGTCTT TACNGTANGG
  661 GCANGTGCNT GCTTCCCNNC NGGNCCTCCN ACNNNNTTGG GGNNNGNCAT CNNGNCNNNT
721 TNNNNNNNNN N
//
Primer: lacIpro-B_F (Pr0724)
Region: lacIq pro,lacI
Sequence file: RDB12833_A4Gpd.seq check
>05699_12833_B1DrS_lacIpro-B_F_H01_22_ABI24.ab1
    1 NNNNNNNNNN NNNNNNGNNN CNNNTCNGTG AGCGAGGAAG CGGAAGAGCG CCTGATGCGG
   61 TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA TAAATTCCGA CACCATCGAA
  121 TGGCGCAAAA CCTTTCGCGG TATGGCATGA TAGCGCCCGG AAGAGAGTCA ATTCAGGGTG
  181 GTGAATGTGA AACCAGTAAC GTTATACGAT GTCGCAGAGT ATGCCGGTGT CTCTTATCAG
  241 ACCGTTTCCC GCGTGGTGAA CCAGGCCAGC CACGTTTCTG CGAAAACGCG GGAAAAAGTG
  301 GAAGCGGCGA TGGCGGAGCT GAATTACATT CCCAACCGCG TGGCACAACA ACTGGCGGGC
  361 AAACAGTCGT TGCTGATTGG CGTTGCCACC TCCAGTCTGG CCCTGCACGC GCCGTCGCAA
  421 ATTGTCGCGG CGATTAAATC TCGCGCCGAT CAACTGGGTG CCAGCGTGGT GGTGTCGATG
  481 GTAGAACGAA GCGGCGTCGA AGCCTGTAAA GCGGCGGTGC ACAATCTTCT CGCGCAACGC
  541 GTCAGTGGGC TGATCATTAA CTATCCGCTG GATGACCAGG ATGCCATTGC TGTGGAANCT
  601 GCCTGCACTA ATGTTCCGGC GTTATTTCTT GATGTCTCTG ANCNNACACC CNTCNACAGT
  661 ATTANTTTCT CCNNTGAANA NNNNACGCGA CNGGGCGNNG AGNNTCNGNT CNNNNNNGNN
721 CNCNNNNNNN NNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

user_report Membrino, A., G4-DNA Formation in the HRAS Promoter and Rational Design of Decoy Oligonucleotides for Cancer Therapy. PLoS One. 6 (9): e24421 (2011). PMID 21931711. [link to RRC of NBRP]