RDB05688 - pRAR RE-TK hRluc(R)

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

pRAR RE-TK hRluc(R) (#RDB05688)

Reporter construct of RAR response element

Alternative name	2489C1R
Clone info.	RAR response element (RARE) is cloned into the BglII site of phRL-TK, and the BglII site is killed by the cloning. The nucleotide sequence of the RARE is 5'-gatccaggtcaaaggtcag-3'. (Ref: J Biol Chem. 269: 890-902, 1994)
Comment	The nucleotide sequence of the element was determined. Function of the inserted element has not been examined by the depositor.
Vector backbone	phRL-TK (Plasmid)
Size of vector backbone	4.0 kb
Selectable markers	Amp^r
Gene/insert name	Artificial RAR response element synthesized
Depositor\|Developer	Yokoyama, Kazunari \|

Distribution information

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Terms and conditions for distribution The RECIPIENT agrees to expressly describe the acknowledgement of the Gene Engineering Division, RIKEN BRC as the source of the BIOLOGICAL RESOURCE in any publication. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for-profit organization for a not-for-profit research.
Additional terms and conditions:
The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.

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Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link]
Terms and conditions for distribution	The RECIPIENT agrees to expressly describe the acknowledgement of the Gene Engineering Division, RIKEN BRC as the source of the BIOLOGICAL RESOURCE in any publication. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for-profit organization for a not-for-profit research. Additional terms and conditions: The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件	リソースを使用して論文を投稿する場合、理研BRC・遺伝子材料開発室から分譲された事を記載する。本件リソースは非営利機関にのみ提供し、非営利研究にのみ利用することができる。 MTAに書く付加的使用条件：本件リソースの使用は学術機関での学術研究に限る。本件リソースの使用にあたって、利用者はプロメガ社の限定使用条件に従う必要がある。

必要書類

提供依頼書

[依頼書の記入例

]
提供同意書 (MTA, 非営利学術目的用)[Word

] [MTAの記入例

]
手続きの概要は、「提供申込みについて[link]」をご覧ください。

MTAに書く使用条件

リソースを使用して論文を投稿する場合、理研BRC・遺伝子材料開発室から分譲された事を記載する。本件リソースは非営利機関にのみ提供し、非営利研究にのみ利用することができる。
MTAに書く付加的使用条件：
本件リソースの使用は学術機関での学術研究に限る。本件リソースの使用にあたって、利用者はプロメガ社の限定使用条件に従う必要がある。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB05688	pRAR RE-TK hRluc(R)	DNA solution

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pRAR RE-TK hRluc(R) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB05688).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB12863_A4It.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: SV40pA3'Element Reverse Sequence file: RDB12863_A4Ita.seq
>05688_12863_A4It_SV40pA3'ElementReverse_D03_12.ab1 1 GATGGCCTGG GGGGGCGGGA CCCTATGGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT 61 TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGGCTCGACA GATCCTGACC TTTGACCTGG 121 ATCTAAATGA GTCTTCGGAC CTCGCGGGGG CCGCTTAAGC GGTGGTTAGG GTTTGTCTGA 181 CGCGGGGGGA GGGGGAAGGA ACGAAACACT CTCATTCGGA GGCGGCTCGG GGTTTGGTCT 241 TGGTGGCCAC GGGCACGCAG AAGAGCGCCG CGATCCTCTT AAGCACCCCC CCGCCCTCCG 301 TGGAGGCGGG GGTTTGGTCG GCGGGTGGTA ACTGGCGGGC CGCTGACTCG GGCGGGTCGC 361 GCGCCCCAGA GTGTGACCTT TTCGGTCTGC TCGCAGACCC CCGGGCGGCG CCGCCGCGGC 421 GGCGACGGGC TCGCTGGGTC CTAGGCTCCA TGGGGACCGT ATACGTGGAC AGGCTCTGGA 481 GCATCCGCAC GACTGCGGTG ATATTACCGG AGACCTTCTG CGGGACGAGC CGGGTCACGC 541 GGCTGACGCG GAGCGTCCGT TGGGCGACAA ACACCAGGAC GGGGCACAGG TACACTATCT 601 TGTCACCCGG AGGCGCGAGG GACTGCAGGA GCTTCAGGGA GTGGCGCAGC TGCTTCATCC 661 CCGTGGCCCG TTGCTCGCGT TTGCTGGCGG TGTCCCCGGA AGAAATATAT TTGCATGTCT 721 TTAGTTCTAT GATGACACAA ACCCCGCCCA GCGTCTTGTC ATTGGCGAAT TCGAAACACG 781 CAGATGCAGT CGGGGCGGCG CGGGTCCCAG GGTCCACTTC GCATATTAAG GGTGACGCGT 841 GTGGCCTCGA AACACCGAGC GACCCCTGCA GCGACCCGCT TTAAAAAGCT TGATTCTTCT 901 GACACAACAG TCTCGAACTT AAGCCTGCAA AAGTTGGGTC GTGAAGGCAC TGGGGCAGGT 961 AAGTATCAAG GTTTACAAGA CAGGGTTTAA AGGAGAACCA TTAGAAACTG GGCTTTGTCG 1021 AGACAGAGAA AAACTTCTTT GCGTTTCTGA TAAGGCCACC CTTATTGGGT CTTAACTTGA 1081 CAATCCCACC TTTTGGCCCT TTTCTTCCTC CACCAGGGTG TTCCCACCTC CCCCAGTTCA 1141 AATTACCAGC CCTCTTTAGG GGCTAGAAGA GTTAACATTT ATAAGTAACG GACTCTGTCA 1201 GTACCTATAT TATGAG //

Primer: SV40pA3'Element Reverse
Sequence file: RDB12863_A4Ita.seq check

>05688_12863_A4It_SV40pA3'ElementReverse_D03_12.ab1
    1 GATGGCCTGG GGGGGCGGGA CCCTATGGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT
   61 TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGGCTCGACA GATCCTGACC TTTGACCTGG
  121 ATCTAAATGA GTCTTCGGAC CTCGCGGGGG CCGCTTAAGC GGTGGTTAGG GTTTGTCTGA
  181 CGCGGGGGGA GGGGGAAGGA ACGAAACACT CTCATTCGGA GGCGGCTCGG GGTTTGGTCT
  241 TGGTGGCCAC GGGCACGCAG AAGAGCGCCG CGATCCTCTT AAGCACCCCC CCGCCCTCCG
  301 TGGAGGCGGG GGTTTGGTCG GCGGGTGGTA ACTGGCGGGC CGCTGACTCG GGCGGGTCGC
  361 GCGCCCCAGA GTGTGACCTT TTCGGTCTGC TCGCAGACCC CCGGGCGGCG CCGCCGCGGC
  421 GGCGACGGGC TCGCTGGGTC CTAGGCTCCA TGGGGACCGT ATACGTGGAC AGGCTCTGGA
  481 GCATCCGCAC GACTGCGGTG ATATTACCGG AGACCTTCTG CGGGACGAGC CGGGTCACGC
  541 GGCTGACGCG GAGCGTCCGT TGGGCGACAA ACACCAGGAC GGGGCACAGG TACACTATCT
  601 TGTCACCCGG AGGCGCGAGG GACTGCAGGA GCTTCAGGGA GTGGCGCAGC TGCTTCATCC
  661 CCGTGGCCCG TTGCTCGCGT TTGCTGGCGG TGTCCCCGGA AGAAATATAT TTGCATGTCT
  721 TTAGTTCTAT GATGACACAA ACCCCGCCCA GCGTCTTGTC ATTGGCGAAT TCGAAACACG
  781 CAGATGCAGT CGGGGCGGCG CGGGTCCCAG GGTCCACTTC GCATATTAAG GGTGACGCGT
  841 GTGGCCTCGA AACACCGAGC GACCCCTGCA GCGACCCGCT TTAAAAAGCT TGATTCTTCT
  901 GACACAACAG TCTCGAACTT AAGCCTGCAA AAGTTGGGTC GTGAAGGCAC TGGGGCAGGT
  961 AAGTATCAAG GTTTACAAGA CAGGGTTTAA AGGAGAACCA TTAGAAACTG GGCTTTGTCG
1021 AGACAGAGAA AAACTTCTTT GCGTTTCTGA TAAGGCCACC CTTATTGGGT CTTAACTTGA
1081 CAATCCCACC TTTTGGCCCT TTTCTTCCTC CACCAGGGTG TTCCCACCTC CCCCAGTTCA
1141 AATTACCAGC CCTCTTTAGG GGCTAGAAGA GTTAACATTT ATAAGTAACG GACTCTGTCA
1201 GTACCTATAT TATGAG
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

Resource search

Link

Other resources in our bank

External Database
Artificial RAR response element

Reference sequence