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pRAR RE-TK hRluc(R)

Reporter construct of RAR response element

Catalog number RDB05688
Resource name pRAR RE-TK hRluc(R)
Alternative name 2489C1R
Clone info. RAR response element (RARE) is cloned into the BglII site of phRL-TK, and the BglII site is killed by the cloning. The nucleotide sequence of the RARE is 5'-gatccaggtcaaaggtcag-3'. (Ref: J Biol Chem. 269: 890-902, 1994)
Comment The nucleotide sequence of the element was determined. Function of the inserted element has not been examined by the depositor.
Vector backbone phRL-TK (Plasmid)
Size of vector backbone 4.0 kb
Selectable markers Amp^r
Gene/insert name Artificial RAR response element synthesized
Depositor|Developer Yokoyama, Kazunari |
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Artificial RAR response element

            Reference sequence
              
             

            Distribution information

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            Terms and conditions for distribution The RECIPIENT agrees to expressly describe the acknowledgement of the Gene Engineering Division, RIKEN BRC as the source of the BIOLOGICAL RESOURCE in any publication. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for-profit organization for a not-for-profit research.
            Additional terms and conditions:
            The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
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            RDB05688 pRAR RE-TK hRluc(R) DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            How to cite this biological resource

            Materials & Methods section:

            The pRAR RE-TK hRluc(R) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB05688).

            Reference section:

            Further references such as user reports and related articles (go to bottom)


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            QC test results

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB12863_A4It.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: SV40pA3'Element Reverse
            Sequence file: RDB12863_A4Ita.seq check
            >05688_12863_A4It_SV40pA3'ElementReverse_D03_12.ab1
                1 GATGGCCTGG GGGGGCGGGA CCCTATGGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT
               61 TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGGCTCGACA GATCCTGACC TTTGACCTGG
              121 ATCTAAATGA GTCTTCGGAC CTCGCGGGGG CCGCTTAAGC GGTGGTTAGG GTTTGTCTGA
              181 CGCGGGGGGA GGGGGAAGGA ACGAAACACT CTCATTCGGA GGCGGCTCGG GGTTTGGTCT
              241 TGGTGGCCAC GGGCACGCAG AAGAGCGCCG CGATCCTCTT AAGCACCCCC CCGCCCTCCG
              301 TGGAGGCGGG GGTTTGGTCG GCGGGTGGTA ACTGGCGGGC CGCTGACTCG GGCGGGTCGC
              361 GCGCCCCAGA GTGTGACCTT TTCGGTCTGC TCGCAGACCC CCGGGCGGCG CCGCCGCGGC
              421 GGCGACGGGC TCGCTGGGTC CTAGGCTCCA TGGGGACCGT ATACGTGGAC AGGCTCTGGA
              481 GCATCCGCAC GACTGCGGTG ATATTACCGG AGACCTTCTG CGGGACGAGC CGGGTCACGC
              541 GGCTGACGCG GAGCGTCCGT TGGGCGACAA ACACCAGGAC GGGGCACAGG TACACTATCT
              601 TGTCACCCGG AGGCGCGAGG GACTGCAGGA GCTTCAGGGA GTGGCGCAGC TGCTTCATCC
              661 CCGTGGCCCG TTGCTCGCGT TTGCTGGCGG TGTCCCCGGA AGAAATATAT TTGCATGTCT
              721 TTAGTTCTAT GATGACACAA ACCCCGCCCA GCGTCTTGTC ATTGGCGAAT TCGAAACACG
              781 CAGATGCAGT CGGGGCGGCG CGGGTCCCAG GGTCCACTTC GCATATTAAG GGTGACGCGT
              841 GTGGCCTCGA AACACCGAGC GACCCCTGCA GCGACCCGCT TTAAAAAGCT TGATTCTTCT
              901 GACACAACAG TCTCGAACTT AAGCCTGCAA AAGTTGGGTC GTGAAGGCAC TGGGGCAGGT
              961 AAGTATCAAG GTTTACAAGA CAGGGTTTAA AGGAGAACCA TTAGAAACTG GGCTTTGTCG
             1021 AGACAGAGAA AAACTTCTTT GCGTTTCTGA TAAGGCCACC CTTATTGGGT CTTAACTTGA
             1081 CAATCCCACC TTTTGGCCCT TTTCTTCCTC CACCAGGGTG TTCCCACCTC CCCCAGTTCA
             1141 AATTACCAGC CCTCTTTAGG GGCTAGAAGA GTTAACATTT ATAAGTAACG GACTCTGTCA
            1201 GTACCTATAT TATGAG
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles


            2023.11.08

            GNP_filter3_RDBDEP_html_231027.pl