Please review the QC test results indicated by icon below as well as clone information before placing your order.

pTR RE-TK hRluc(F) (#RDB05630)

Reporter construct of TR response element

Alternative name	2485C3F
Clone info.	Thyroid hormone response element (TRE-DR4) is cloned into the BglII site of phRL-TK, and the BglII site is killed by the cloning. The nucleotide sequence of the TRE is 5'-gatccaggtcaaaaaaggtcag-3'. (Ref: Endocrinol. 135: 1628-1638, 1994)
Comment	The nucleotide sequence of the element was determined. Function of the inserted element has not been examined by the depositor.
Vector backbone	phRL-TK (Plasmid)
Size of vector backbone	4.0 kb
Selectable markers	Amp^r
Gene/insert name	Artificial TR response element synthesized
Depositor|Developer	Yokoyama, Kazunari |

 

Remarks, protocol and/or map (pdf)	RDB05630.pdf

Distribution information

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Terms and conditions for distribution

The RECIPIENT agrees to expressly describe the acknowledgement of the Gene Engineering Division, RIKEN BRC as the source of the BIOLOGICAL RESOURCE in any publication. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for-profit organization for a not-for-profit research. Additional terms and conditions: The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.

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Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB05630	pTR RE-TK hRluc(F)	DNA solution

Please review the QC test results indicated by icon below as well as clone information before placing your order.

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How to cite this biological resource

Materials & Methods section:

The pTR RE-TK hRluc(F) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB05630).

Reference section:

Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB16935_A9C4p1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: SV40pA3'ElementReverse () Sequence file: RDB16935_A9C4a.seq
>05630_16935_A9C4_3_SV40pA3'ElementReverse_A02_01_ABI08.ab1     1 GGTCGGTTTG GGGGGGGCGG AGCCTATGGA AAACGCCAGC AACGCGGCCT TTTTACGGTT    61 CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GGCTCGACAG ATCCAGGTCA AAAAAGGTCA   121 GGATCTAAAT GAGTCTTCGG ACCTCGCGGG GGCCGCTTAA GCGGTGGTTA GGGTTTGTCT   181 GACGCGGGGG GAGGGGGAAG GAACGAAACA CTCTCATTCG GAGGCGGCTC GGGGTTTGGT   241 CTTGGTGGCC ACGGGCACGC AGAAGAGCGC CGCGATCCTC TTAAGCACCC CCCCGCCCTC   301 CGTGGAGGCG GGGGTTTGGT CGGCGGGTGG TAACTGGCGG GCCGCTGACT CGGGCGGGTC   361 GCGCGCCCCA GAGTGTGACC TTTTCGGTCT GCTCGCAGAC CCCCGGGCGG CGCCGCCGCG   421 GCGGCGACGG GCTCGCTGGG TCCTAGGCTC CATGGGGACC GTATACGTGG ACAGGCTCTG   481 GAGCATCCGC ACGACTGCGG TGATATTACC GGAGACCTTC TGCGGGACGA GCCGGGTCAC   541 GCGGCTGACG CGGAGCGTCC GTTGGGCGAC AAACACCAGG ACGGGGCACA GGTACACTAT   601 CTTGTCACCC GGAGGCGCGA GGGACTGCAG GAGCTTCAGG GAGTGGCGCA GCTGCTTCAT   661 CCCCGTGGCC CGTTGCTCGC GTTTGCTGGC GGTGTCCCCG GAAGAAATAT ATTTGCATGT 721 CTTTAGTTCT ATGATGACTC CAACCCCGCC CAGCGTCCTT GT //
Primer: Tkpro-F () Sequence file: RDB16935_A9C4b.seq
>05630_16935_A9C4_3_Tkpro-F_B02_02_ABI08.ab1     1 CGGGGTGCCT CGAACCCGAG CGACCCTGCA GCGACCCGCT TAAAAGCTTG ATTCTTCTGA    61 CACAACAGTC TCGAACTTAA GCTGCAGAAG TTGGTCGTGA GGCACTGGGC AGGTAAGTAT   121 CAAGGTTACA AGACAGGTTT AAGGAGACCA ATAGAAACTG GGCTTGTCGA GACAGAGAAG   181 ACTCTTGCGT TTCTGATAGG CACCTATTGG TCTTACTGAC ATCCACTTTG CCTTTCTCTC   241 CACAGGTGTC CACTCCCAGT TCAATTACAG CTCTTAAGGC TAGAGTACTT AATACGACTC   301 ACTATAGGCT AGCCACCATG GCTTCCAAGG TGTACGACCC CGAGCAACGC AAACGCATGA   361 TCACTGGGCC TCAGTGGTGG GCTCGCTGCA AGCAAATGAA CGTGCTGGAC TCCTTCATCA   421 ACTACTATGA TTCCGAGAAG CACGCCGAGA ACGCCGTGAT TTTTCTGCAT GGTAACGCTG   481 CCTCCAGCTA CCTGTGGAGG CACGTCGTGC CTCACATCGA GCCCGTGGCT AGATGCATCA   541 TCCCTGATCT GATCGGAATG GGTAAGTCCG GCAAGAGCGG GAATGGCTCA TATCGCCTCC   601 TGGATCACTA CAAGTACCTC ACCGCTTGGT TCGAGCTGCT GAACCTTCCA AAGAAAATCA   661 TCTTTGTGGG CCACGACTGG GGGGCTTGTC TGGCCTTTCA CTACTCCTAC GAGCACCAAG   721 ACAAGATCAA GGCCATCGTC CATGCTGAGA GTGTCGTGGA CGTGATCGAG TCCTGGGACG   781 AGTGGCCTGA CATCGAGGAG GATATCGCCC TGATCAAGAG CGAAAGAGGG CGAGAAAATG   841 GTGCTTGAGA ATAACTTTCT TCGTCGAGAC CATGCTCCCA AGCAAGATCA TGCGGAAACT   901 GGGAGCCTGA GGAGTTCGCT GCCTACCTGG AGGCCATTCA AGGAGAAGGG CGAGGTTAGA   961 CGGCCTACCC TCTCCTGGCC TCGCTAGATC CCCTCTCGTT AAGGGAGGAA GCCCGACGTC 1021 GTCCAAATTG TCCGCAACTA CAACGCCTAC CCTTCCGGGC CCGCGACCAA TCTGGCTT //
Primer: pGCAP_R () Sequence file: RDB16935_A9C4c.seq
>05630_16935_A9C4_3_pGCAP_R_C02_03_ABI08.ab1     1 CGGGAAACTT ATCATGTCTG CTCGAAGCGG CCGCTCTAGA ATTACTGCTC GTTCTTCAGC    61 ACGCGCTCCA CGAAGCTCTT GATGTACTTA CCCATTTCAT CTGGAGCGTC CTCCTGGCTG   121 AAGTGGAGGC CCTTCACCTT CACGAACTCG GTGTTAGGGA ACTTCTTAGC TCCCTCGACA   181 ATAGCGTTGG AAAAGAACCC AGGGTCGGAC TCGATGAACA TCTTAGGCAG ATCGTCGCTG   241 GCCCGAAGGT AGGCGTTGTA GTTGCGGACA ATCTGGACGA CGTCGGGCTT GCCTCCCTTA   301 ACGAGAGGGA TCTCGCGAGG CCAGGAGAGG GTAGGCCGTC TAACCTCGCC CTTCTCCTTG   361 AATGGCTCCA GGTAGGCAGC GAACTCCTCA GGCTCCAGTT TCCGCATGAT CTTGCTTGGG   421 AGCATGGTCT CGACGAAGAA GTTATTCTCA AGCACCATTT TCTCGCCCTC TTCGCTCTTG   481 ATCAGGGCGA TATCCTCCTC GATGTCAGGC CACTCGTCCC AGGACTCGAT CACGTCCACG   541 ACACTCTCAG CATGGACGAT GGCCTTGATC TTGTCTTGGT GCTCGTAGGA GTAGTGAAAG   601 GCCAGACAAG CCCCCCAGTC GTGGCCCACA AAGATGATTT TCTTTGGAAG GTTCAGCAGC   661 TCGAACCAAG CGGTGAGGTA CTTGTAGTGA TCCAGGAGGC GATATGAGCC ATTCCCGCTC   721 TTGCCGGACT TACCCATTCC GATCAGATCA GGGATGATGC ATCTAGCCAC GGGCTCGATG   781 TGAGGCACGA CGTGCCTCCA CAGGTAGCTG GAGGCAGCGT TACCATGCAG AAAAATCACG   841 GCGTTCTCGG CGTGCTTCTC GGAATCATAG TAGTTGATGA AGGGAGTCCA GCACGTTCAT   901 TTGCTTGCAG CGAGCCCACC ACTGAGGCCC AAGTGATCAT GCGTTTGCGT TGCTCGGGGT   961 TCGTACACCT TGGAAAGCCA TGGTGGGCTA GCCCTATAGT GAAGTCGTAT TTAAGTACTC  1021 TTAGCCTTTA AGAGCTGGTA TTTGAACTGG GAAGTGGAAC ACCTGTTGAA GAGAAAGGCA  1081 AAGTGGGATG TCAGTAGAAC AAATAGGCTG CCTATTCAGA ACCGCAAGAA AGTCTGTGTC  1141 TGGTCCTCGA CCAGCCCAAT TCTATTTGGT CTTCCGTTAA CCTTGTCTGT GTAACTCTGG 1201 ATTACCCTAC CCTGTCCCGA TGGCGGCCGC TCT //

Please visit Sequencing and PCR primers for primer information.

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References

Original, user report and related articles

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Artificial TR response element

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