Please review the QC test results indicated by icon below as well as clone information before placing your order.

pmutant GR RE-TK hRluc(F) (#RDB05629)

Reporter construct of GR (MR,PR,AR) response element, mutated

Alternative name	2484C6F
Clone info.	mutant GR (MR,PR,AR) response element is cloned into the BglII site of phRL-TK, and the BglII site is killed by the cloning. The nucleotide sequence of the mutant GR (MR,PR,AR) response element is 5'-gatccagcccaaaatgaactg-3'.
Comment	The nucleotide sequence of the element was determined. Function of the inserted element has not been examined by the depositor.
Vector backbone	phRL-TK (Plasmid)
Size of vector backbone	4.0 kb
Selectable markers	Amp^r
Gene/insert name	Artificial GR (MR,PR,AR) response element, mutated synthesized
Depositor|Developer	Yokoyama, Kazunari |

 

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The RECIPIENT agrees to expressly describe the acknowledgement of the Gene Engineering Division, RIKEN BRC as the source of the BIOLOGICAL RESOURCE in any publication. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for-profit organization for a not-for-profit research. Additional terms and conditions: The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.

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Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB05629	pmutant GR RE-TK hRluc(F)	DNA solution

Please review the QC test results indicated by icon below as well as clone information before placing your order.

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How to cite this biological resource

Materials & Methods section:

The pmutant GR RE-TK hRluc(F) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB05629).

Reference section:

Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB15034_A7Ecp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: SV40pA3'Element Reverse Sequence file: RDB15034_A7Eca.seq
>05629_15034_A7Ec_Sv40pA3'ElementReverse_B04_04_ABI24.ab1     1 TACGCAGGGG GCGGAGCCTA TGGAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC    61 CTTTTGCTGG CCTTTTGCTC ACATGGCTCG ACAGATCCAG CCCAAAATGA ACTGGATCTA   121 AATGAGTCTT CGGACCTCGC GGGGGCCGCT TAAGCGGTGG TTAGGGTTTG TCTGACGCGG   181 GGGGAGGGGG AAGGAACGAA ACACTCTCAT TCGGAGGCGG CTCGGGGTTT GGTCTTGGTG   241 GCCACGGGCA CGCAGAAGAG CGCCGCGATC CTCTTAAGCA CCCCCCCGCC CTCCGTGGAG   301 GCGGGGGTTT GGTCGGCGGG TGGTAACTGG CGGGCCGCTG ACTCGGGCGG GTCGCGCGCC   361 CCAGAGTGTG ACCTTTTCGG TCTGCTCGCA GACCCCCGGG CGGCGCCGCC GCGGCGGCGA   421 CGGGCTCGCT GGGTCCTAGG CTCCATGGGG ACCGTATACG TGGACAGGCT CTGGAGCATC   481 CGCACGACTG CGGTGATATT ACCGGAGACC TTCTGCGGGA CGAGCCGGGT CACGCGGCTG   541 ACGCGGAGCG TCCGTTGGGC GACAAACACC AGGACGGGGC ACAGGTACAC TATCTTGTCA   601 CCCGGAGGCG CGAGGGACTG CAGGAGCTTC AGGGAGTGGC GCAGCTGCTT CATCCCCGTG   661 GCCCGTTGCT CGCGTTTGCT GGCGGTGTCC CCGGAAGAAA TATATTTGCA TGTCTTTAGT   721 TCTATGATGA CACAAACCCC GCCCAGCGTC TTGTCATTGG CGAATTCGAA CACGCAGATG   781 CAGTCGGGGC GGCGCGGTCC CAGGTCCACT TCGCATATTA AGGTGACGCG TGTGGCCTCG   841 AACACCGAGC GACCCTGCAG CGACCCGCTT AAAAGCTTGA TTCTTCTGAC ACAACAGTCT   901 CGAACTTAAG CTGCAGAAGT TGGTCGTGAG GCACTGGGCA GGTAAGTATC AAGGTTACAA   961 GACAGGTTTA AGGAGACCAA TAGAACTGGG CTTGTCGAGA CAGAGAAGAC TCTTGCGTTT  1021 CTGATAGGCA CCTATTGGTC TTACTGACAT CCACTTGCTT TCTCCTCCAC AGGTGTCCAC  1081 TCCCAGTCCA TACAGCTCTA AGGCTAGAGT ACCTATACGA CTCACTATAG GCTAGCCAAC 1141 CATGGACTTC CCGAGGGTGT TACCGAATCC A //
Primer: pAxCALNL_F1 Sequence file: RDB15034_A7Ecb.seq
>05629_15034_A7Ec_pAxCALNL_F1_C04_07_ABI24.ab1     1 ACTATATATC TTATCATGTC TGCTCGAAGC GGCCGCTCTA GAATTACTGC TCGTTCTTCA    61 GCACGCGCTC CACGAAGCTC TTGATGTACT TACCCATTTC ATCTGGAGCG TCCTCCTGGC   121 TGAAGTGGAG GCCCTTCACC TTCACGAACT CGGTGTTAGG GAACTTCTTA GCTCCCTCGA   181 CAATAGCGTT GGAAAAGAAC CCAGGGTCGG ACTCGATGAA CATCTTAGGC AGATCGTCGC   241 TGGCCCGAAG GTAGGCGTTG TAGTTGCGGA CAATCTGGAC GACGTCGGGC TTGCCTCCCT   301 TAACGAGAGG GATCTCGCGA GGCCAGGAGA GGGTAGGCCG TCTAACCTCG CCCTTCTCCT   361 TGAATGGCTC CAGGTAGGCA GCGAACTCCT CAGGCTCCAG TTTCCGCATG ATCTTGCTTG   421 GGAGCATGGT CTCGACGAAG AAGTTATTCT CAAGCACCAT TTTCTCGCCC TCTTCGCTCT   481 TGATCAGGGC GATATCCTCC TCGATGTCAG GCCACTCGTC CCAGGACTCG ATCACGTCCA   541 CGACACTCTC AGCATGGACG ATGGCCTTGA TCTTGTCTTG GTGCTCGTAG GAGTAGTGAA   601 AGGCCAGACA AGCCCCCCAG TCGTGGCCCA CAAAGATGAT TTTCTTTGGA AGGTTCAGCA   661 GCTCGAACCA AGCGGTGAGG TACTTGTAGT GATCCAGGAG GCGATATGAG CCATTCCCGC   721 TCTTGCCGGA CTTACCCATT CCGATCAGAT CAGGGATGAT GCATCTAGCC ACGGGCTCGA   781 TGTGAGGCAC GACGTGCCTC CACAGGTAGC TGGAGGCAGC GTTACCATGC AGAAAAATCA   841 CGGCGTTCTC GGCGTGCTTC TCGGAATCAT AGTAGTTGAT GAAGGAGTCC AGCACGTTCA   901 TTTGCTTGCA GCGAGCCCAC CACTGAGGCC CAGTGATCAT GCGTTTGCGT TGCTCGGGGT   961 CGTACACCTT GGAAAGCCAT GGTGGCTAGC CTATAGTGAG TCGTATTAAG TACTCTAGCC  1021 TTAAGAGCTG TAATTGAACT GGGGAGTGGA CACCTGGTGG GAGAGAACGC AAAGTGGATG  1081 TCAGTAGACC CAATAGTGCC TATCAGAACG CAGAGTCTTC CCTGGTCTCG ACAGCCAGTT 1141 CGATGGATCT CCTTAACCTG TTCTTGTGTT AACCCTGGAA TATACCT //

Please visit Sequencing and PCR primers for primer information.

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References

Original, user report and related articles

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Artificial GR (MR,PR,AR) response element, mutated

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