Please review the QC test results indicated by icon below as well as clone information before placing your order.

pGR RE-TK hRluc(F) (#RDB05628)

Reporter construct of GR (MR,PR,AR) response element

Alternative name	2483C7F
Clone info.	GR (MR,PR,AR) response element is cloned into the BglII site of phRL-TK, and the BglII site is killed by the cloning. The nucleotide sequence of the GR (MR,PR,AR) response element is 5'-gatccagaacaaaatgttctg-3'.
Comment	The nucleotide sequence of the element was determined. Function of the inserted element has not been examined by the depositor.
Vector backbone	phRL-TK (Plasmid)
Size of vector backbone	4.0 kb
Selectable markers	Amp^r
Gene/insert name	Artificial GR (MR,PR,AR) response element synthesized
Depositor|Developer	Yokoyama, Kazunari |

 

Remarks, protocol and/or map (pdf)	RDB05628.pdf

Distribution information

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Terms and conditions for distribution

The RECIPIENT agrees to expressly describe the acknowledgement of the Gene Engineering Division, RIKEN BRC as the source of the BIOLOGICAL RESOURCE in any publication. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for-profit organization for a not-for-profit research. Additional terms and conditions: The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.

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Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB05628	pGR RE-TK hRluc(F)	DNA solution

Please review the QC test results indicated by icon below as well as clone information before placing your order.

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How to cite this biological resource

Materials & Methods section:

The pGR RE-TK hRluc(F) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB05628).

Reference section:

Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB14286_A6H2p1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: SV40pA3'Element Reverse Sequence file: RDB14286_A6H2a.seq
>05628_14286_A6H2_3_SV40pA3ElementReverse_C12_09.ab1     1 GAGTCTAGGG GGCGGACCTA TGGAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC    61 TTTTGCTGGC CTTTTGCTCA CATGGCTCGA CAGATCCAGA ACAAAATGTT CTGGATCTAA   121 ATGAGTCTTC GGACCTCGCG GGGGCCGCTT AAGCGGTGGT TAGGGTTTGT CTGACGCGGG   181 GGGAGGGGGA AGGAACGAAA CACTCTCATT CGGAGGCGGC TCGGGGTTTG GTCTTGGTGG   241 CCACGGGCAC GCAGAAGAGC GCCGCGATCC TCTTAAGCAC CCCCCCGCCC TCCGTGGAGG   301 CGGGGGTTTG GTCGGCGGGT GGTAACTGGC GGGCCGCTGA CTCGGGCGGG TCGCGCGCCC   361 CAGAGTGTGA CCTTTTCGGT CTGCTCGCAG ACCCCCGGGC GGCGCCGCCG CGGCGGCGAC   421 GGGCTCGCTG GGTCCTAGGC TCCATGGGGA CCGTATACGT GGACAGGCTC TGGAGCATCC   481 GCACGACTGC GGTGATATTA CCGGAGACCT TCTGCGGGAC GAGCCGGGTC ACGCGGCTGA   541 CGCGGAGCGT CCGTTGGGCG ACAAACACCA GGACGGGGCA CAGGTACACT ATCTTGTCAC   601 CCGGAGGCGC GAGGGACTGC AGGAGCTTCA GGGAGTGGCG CAGCTGCTTC ATCCCCGTGG   661 CCCGTTGCTC GCGTTTGCTG GCGGTGTCCC CGGAAGAAAT ATATTTGCAT GTCTTTAGTT   721 CTATGATGAC ACAAACCCCG CCCAGCGTCT TGTCATTGGC GAATTCGAAC ACGCAGATGC   781 AGTCGGGGCG GCGCGGTCCC AGGTCCACTT CGCATATTAA GGTGACGCGT GTGGCCTCGA   841 ACACCGAGCG ACCCTGCAGC GACCCGCTTA AAAGCTTGAT TCTTCTGACA CAACAGTCTC   901 GAACTTAAGC TGCAGAAGTT GGTCGTGAGG CACTGGGCAG GTAAGTATCA AAGGTTACAA   961 GACAGGTTTA AGGAGACCCA ATAGAAACTG GGCTTGTCGA GACAGGAGAA GACTCTGCGT  1021 TCTGATAGGC ACCTATGGTC TACTGACATC CACTTGCTTT CTCTCCACAG GTGTCCACCT  1081 CCCAGTCATA CAGCTCGTAG GCTAGAGTAC CTATTACGAA CTTCACTATA AGCTAGCTCT 1141 CATCTATGGT CT //
Primer: pAxCALNL_F1 Sequence file: RDB14286_A6H2b.seq
>05628_14286_A6H2_3_pAxCALNL_F1_D12_12.ab1     1 ATAATCTATC TTATCATGTC TGCTCGAAGC GGCCGCTCTA GAATTACTGC TCGTTCTTCA    61 GCACGCGCTC CACGAAGCTC TTGATGTACT TACCCATTTC ATCTGGAGCG TCCTCCTGGC   121 TGAAGTGGAG GCCCTTCACC TTCACGAACT CGGTGTTAGG GAACTTCTTA GCTCCCTCGA   181 CAATAGCGTT GGAAAAGAAC CCAGGGTCGG ACTCGATGAA CATCTTAGGC AGATCGTCGC   241 TGGCCCGAAG GTAGGCGTTG TAGTTGCGGA CAATCTGGAC GACGTCGGGC TTGCCTCCCT   301 TAACGAGAGG GATCTCGCGA GGCCAGGAGA GGGTAGGCCG TCTAACCTCG CCCTTCTCCT   361 TGAATGGCTC CAGGTAGGCA GCGAACTCCT CAGGCTCCAG TTTCCGCATG ATCTTGCTTG   421 GGAGCATGGT CTCGACGAAG AAGTTATTCT CAAGCACCAT TTTCTCGCCC TCTTCGCTCT   481 TGATCAGGGC GATATCCTCC TCGATGTCAG GCCACTCGTC CCAGGACTCG ATCACGTCCA   541 CGACACTCTC AGCATGGACG ATGGCCTTGA TCTTGTCTTG GTGCTCGTAG GAGTAGTGAA   601 AGGCCAGACA AGCCCCCCAG TCGTGGCCCA CAAAGATGAT TTTCTTTGGA AGGTTCAGCA   661 GCTCGAACCA AGCGGTGAGG TACTTGTAGT GATCCAGGAG GCGATATGAG CCATTCCCGC   721 TCTTGCCGGA CTTACCCATT CCGATCAGAT CAGGGATGAT GCATCTAGCC ACGGGCTCGA   781 TGTGAGGCAC GACGTGCCTC CACAGGTAGC TGGAGGCAGC GTTACCATGC AGAAAAATCA   841 CGGCGTTCTC GGCGTGCTTC TCGGAATCAT AGTAGTTGAT GAAGGAGTCC AGCACGTTCA   901 TTTGCTTGCA GCGAGCCCAC CACTGAGGCC CAGTGATCAT GCGTTTGCGT TGCTCGGGGT   961 CGTACACCTT GGGAAGCCAT GGTGGCTAGC CTATAGTGAG TCGTATTAAG TACTCTAGCC  1021 CTTAGAGCTG TAATTGAACT GGGGAGTGGA CACCTGTGGA GAGAAAGCAA AGTGGATGTC  1081 AGTAGACATT AGTTGCTATC AGAAACGCAG AGTCTTCCTG TCTCGGACAG GCCAGTTCTA 1141 TTGATCCTCC TTAAACCTGT TCTTTGTGTT AC //

Please visit Sequencing and PCR primers for primer information.

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References

Original, user report and related articles

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Artificial GR (MR,PR,AR) response element

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