Please review the QC test results indicated by icon below as well as clone information before placing your order.

pmutant Smad RE-TK hRluc(F) (#RDB05623)

Reporter construct of SMAD response element, mutated

Alternative name	2478C6F
Clone info.	mutant Smad response element is cloned into the BglII site of phRL-TK, and the BglII site is killed by the cloning. The nucleotide sequence of the mutant Smad response element is 5'-gatccgacttcacgacttcacg-3'. (Ref: Mol Cell 1: 611-617, 1998)
Comment	The nucleotide sequence of the element was determined. Function of the inserted element has not been examined by the depositor.
Vector backbone	phRL-TK (Plasmid)
Size of vector backbone	4.0 kb
Selectable markers	Amp^r
Gene/insert name	Artificial Smad response element, mutated synthesized
Depositor|Developer	Yokoyama, Kazunari |

 

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The RECIPIENT agrees to expressly describe the acknowledgement of the Gene Engineering Division, RIKEN BRC as the source of the BIOLOGICAL RESOURCE in any publication. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for-profit organization for a not-for-profit research. Additional terms and conditions: The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.

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Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB05623	pmutant Smad RE-TK hRluc(F)	DNA solution

Please review the QC test results indicated by icon below as well as clone information before placing your order.

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How to cite this biological resource

Materials & Methods section:

The pmutant Smad RE-TK hRluc(F) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB05623).

Reference section:

Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB13758_A6A8p1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: SV40pA3'Element Reverse Sequence file: RDB13758_A6A8a.seq
>05623_13758_A6A8_SV40pA3'ElementReverse_C02_08.ab1     1 TTGGCAGGGG CGGACCTATG GAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT    61 TTGCTGGCCT TTTGCTCACA TGGCTCGACA GATCCGACTT CACGACTTCA CGGATCTAAA   121 TGAGTCTTCG GACCTCGCGG GGGCCGCTTA AGCGGTGGTT AGGGTTTGTC TGACGCGGGG   181 GGAGGGGGAA GGAACGAAAC ACTCTCATTC GGAGGCGGCT CGGGGTTTGG TCTTGGTGGC   241 CACGGGCACG CAGAAGAGCG CCGCGATCCT CTTAAGCACC CCCCCGCCCT CCGTGGAGGC   301 GGGGGTTTGG TCGGCGGGTG GTAACTGGCG GGCCGCTGAC TCGGGCGGGT CGCGCGCCCC   361 AGAGTGTGAC CTTTTCGGTC TGCTCGCAGA CCCCCGGGCG GCGCCGCCGC GGCGGCGACG   421 GGCTCGCTGG GTCCTAGGCT CCATGGGGAC CGTATACGTG GACAGGCTCT GGAGCATCCG   481 CACGACTGCG GTGATATTAC CGGAGACCTT CTGCGGGACG AGCCGGGTCA CGCGGCTGAC   541 GCGGAGCGTC CGTTGGGCGA CAAACACCAG GACGGGGCAC AGGTACACTA TCTTGTCACC   601 CGGAGGCGCG AGGGACTGCA GGAGCTTCAG GGAGTGGCGC AGCTGCTTCA TCCCCGTGGC   661 CCGTTGCTCG CGTTTGCTGG CGGTGTCCCC GGAAGAAATA TATTTGCATG TCTTTAGTTC   721 TATGATGACA CAAACCCCGC CCAGCGTCTT GTCATTGGCG AATTCGAACA CGCAGATGCA   781 GTCGGGGCGG CGCGGTCCCA GGTCCACTTC GCATATTAAG GTGACGCGTG TGGCCTCGAA   841 CACCGAGCGA CCCTGCAGCG ACCCGCTTAA AAGCTTGATT CTTCTGACAC AACAGTCTCG   901 AACTTAAGCT GCAGAAGTTG GGTCGTGAGG CACTGGGCCA GGTAAGTATC AAAGGTTACA   961 AGACAGGTTT AAAGGAGACC AATAGAAACT GGGCTTGTCG AGACAGAGAA GACTCTTGCG  1021 TTTCTGATAG GCACCTAATT GGTCTACTGA ACATCAACTT TGGCCTTTCT CTTCCACAGT  1081 GTCACTCCAA GTTCATTACA GCTCTAGGCT GAAAGTACCT ATACCGACCT CACTAATAGG 1141 GCCTAAGCCA CCCAGATG //
Primer: pAxCALNL_F1 Sequence file: RDB13758_A6A8b.seq
>05623_13758_A6A8_pAxCALNL_F1_D02_11.ab1     1 ATAATGTATC TTATCATGTC TGCTCGAAGC GGCCGCTCTA GAATTACTGC TCGTTCTTCA    61 GCACGCGCTC CACGAAGCTC TTGATGTACT TACCCATTTC ATCTGGAGCG TCCTCCTGGC   121 TGAAGTGGAG GCCCTTCACC TTCACGAACT CGGTGTTAGG GAACTTCTTA GCTCCCTCGA   181 CAATAGCGTT GGAAAAGAAC CCAGGGTCGG ACTCGATGAA CATCTTAGGC AGATCGTCGC   241 TGGCCCGAAG GTAGGCGTTG TAGTTGCGGA CAATCTGGAC GACGTCGGGC TTGCCTCCCT   301 TAACGAGAGG GATCTCGCGA GGCCAGGAGA GGGTAGGCCG TCTAACCTCG CCCTTCTCCT   361 TGAATGGCTC CAGGTAGGCA GCGAACTCCT CAGGCTCCAG TTTCCGCATG ATCTTGCTTG   421 GGAGCATGGT CTCGACGAAG AAGTTATTCT CAAGCACCAT TTTCTCGCCC TCTTCGCTCT   481 TGATCAGGGC GATATCCTCC TCGATGTCAG GCCACTCGTC CCAGGACTCG ATCACGTCCA   541 CGACACTCTC AGCATGGACG ATGGCCTTGA TCTTGTCTTG GTGCTCGTAG GAGTAGTGAA   601 AGGCCAGACA AGCCCCCCAG TCGTGGCCCA CAAAGATGAT TTTCTTTGGA AGGTTCAGCA   661 GCTCGAACCA AGCGGTGAGG TACTTGTAGT GATCCAGGAG GCGATATGAG CCATTCCCGC   721 TCTTGCCGGA CTTACCCATT CCGATCAGAT CAGGGATGAT GCATCTAGCC ACGGGCTCGA   781 TGTGAGGCAC GACGTGCCTC CACAGGTAGC TGGAGGCAGC GTTACCATGC AGAAAAATCA   841 CGGCGTTCTC GGCGTGCTTC TCGGAATCAT AGTAGTTGAT GAAGGAGTCC AGCACGTTCA   901 TTTGCTTGCA GCGAGCCCAC CACTGAGGCC CAGTGATCAT GCGTTTGCGT TGCTCGGGGT   961 CGTACACCTT GGAAGCCATG GGTGGCTAGC CTATAGTGAG TCGTATTAAG TACTCTAGCC  1021 TTAAGAGCTG TAATTGAACT GGGGAGTGGA CACTGTGAGA GAAGGCAAGT GGATGTCAGT  1081 TAGACATTAG GTGCTATCAG AAACGCAGAG TCTTCTCTGG TCTCGGACAG CCCAGTTCTA 1141 TTGATCCTCC TTAAACCTGA TCCTT //

Please visit Sequencing and PCR primers for primer information.

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References

Original, user report and related articles

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Artificial Smad response element, mutated

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