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pmutant Smad RE-TK hRluc(F)

Reporter construct of SMAD response element, mutated

Catalog number RDB05623
Resource name pmutant Smad RE-TK hRluc(F)
Alternative name 2478C6F
Clone info. mutant Smad response element is cloned into the BglII site of phRL-TK, and the BglII site is killed by the cloning. The nucleotide sequence of the mutant Smad response element is 5'-gatccgacttcacgacttcacg-3'. (Ref: Mol Cell 1: 611-617, 1998)
Comment The nucleotide sequence of the element was determined. Function of the inserted element has not been examined by the depositor.
Vector backbone phRL-TK (Plasmid)
Size of vector backbone 4.0 kb
Selectable markers Amp^r
Gene/insert name Artificial Smad response element, mutated synthesized
Depositor|Developer Yokoyama, Kazunari |
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Artificial Smad response element, mutated
            Reference sequence
              
             

            Distribution information

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            Terms and conditions for distribution The RECIPIENT agrees to expressly describe the acknowledgement of the Gene Engineering Division, RIKEN BRC as the source of the BIOLOGICAL RESOURCE in any publication. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for-profit organization for a not-for-profit research.
            Additional terms and conditions:
            The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
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            RDB05623 pmutant Smad RE-TK hRluc(F) DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            How to cite this biological resource

            Materials & Methods section:

            The pmutant Smad RE-TK hRluc(F) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB05623).

            Reference section:

            Further references such as user reports and related articles (go to bottom)

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            QC test results

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB13758_A6A8p1.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: SV40pA3'Element Reverse
            Sequence file: RDB13758_A6A8a.seq check
            >05623_13758_A6A8_SV40pA3'ElementReverse_C02_08.ab1
                1 TTGGCAGGGG CGGACCTATG GAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT
               61 TTGCTGGCCT TTTGCTCACA TGGCTCGACA GATCCGACTT CACGACTTCA CGGATCTAAA
              121 TGAGTCTTCG GACCTCGCGG GGGCCGCTTA AGCGGTGGTT AGGGTTTGTC TGACGCGGGG
              181 GGAGGGGGAA GGAACGAAAC ACTCTCATTC GGAGGCGGCT CGGGGTTTGG TCTTGGTGGC
              241 CACGGGCACG CAGAAGAGCG CCGCGATCCT CTTAAGCACC CCCCCGCCCT CCGTGGAGGC
              301 GGGGGTTTGG TCGGCGGGTG GTAACTGGCG GGCCGCTGAC TCGGGCGGGT CGCGCGCCCC
              361 AGAGTGTGAC CTTTTCGGTC TGCTCGCAGA CCCCCGGGCG GCGCCGCCGC GGCGGCGACG
              421 GGCTCGCTGG GTCCTAGGCT CCATGGGGAC CGTATACGTG GACAGGCTCT GGAGCATCCG
              481 CACGACTGCG GTGATATTAC CGGAGACCTT CTGCGGGACG AGCCGGGTCA CGCGGCTGAC
              541 GCGGAGCGTC CGTTGGGCGA CAAACACCAG GACGGGGCAC AGGTACACTA TCTTGTCACC
              601 CGGAGGCGCG AGGGACTGCA GGAGCTTCAG GGAGTGGCGC AGCTGCTTCA TCCCCGTGGC
              661 CCGTTGCTCG CGTTTGCTGG CGGTGTCCCC GGAAGAAATA TATTTGCATG TCTTTAGTTC
              721 TATGATGACA CAAACCCCGC CCAGCGTCTT GTCATTGGCG AATTCGAACA CGCAGATGCA
              781 GTCGGGGCGG CGCGGTCCCA GGTCCACTTC GCATATTAAG GTGACGCGTG TGGCCTCGAA
              841 CACCGAGCGA CCCTGCAGCG ACCCGCTTAA AAGCTTGATT CTTCTGACAC AACAGTCTCG
              901 AACTTAAGCT GCAGAAGTTG GGTCGTGAGG CACTGGGCCA GGTAAGTATC AAAGGTTACA
              961 AGACAGGTTT AAAGGAGACC AATAGAAACT GGGCTTGTCG AGACAGAGAA GACTCTTGCG
             1021 TTTCTGATAG GCACCTAATT GGTCTACTGA ACATCAACTT TGGCCTTTCT CTTCCACAGT
             1081 GTCACTCCAA GTTCATTACA GCTCTAGGCT GAAAGTACCT ATACCGACCT CACTAATAGG
            1141 GCCTAAGCCA CCCAGATG
            //
            Primer: pAxCALNL_F1
            Sequence file: RDB13758_A6A8b.seq check
            >05623_13758_A6A8_pAxCALNL_F1_D02_11.ab1
                1 ATAATGTATC TTATCATGTC TGCTCGAAGC GGCCGCTCTA GAATTACTGC TCGTTCTTCA
               61 GCACGCGCTC CACGAAGCTC TTGATGTACT TACCCATTTC ATCTGGAGCG TCCTCCTGGC
              121 TGAAGTGGAG GCCCTTCACC TTCACGAACT CGGTGTTAGG GAACTTCTTA GCTCCCTCGA
              181 CAATAGCGTT GGAAAAGAAC CCAGGGTCGG ACTCGATGAA CATCTTAGGC AGATCGTCGC
              241 TGGCCCGAAG GTAGGCGTTG TAGTTGCGGA CAATCTGGAC GACGTCGGGC TTGCCTCCCT
              301 TAACGAGAGG GATCTCGCGA GGCCAGGAGA GGGTAGGCCG TCTAACCTCG CCCTTCTCCT
              361 TGAATGGCTC CAGGTAGGCA GCGAACTCCT CAGGCTCCAG TTTCCGCATG ATCTTGCTTG
              421 GGAGCATGGT CTCGACGAAG AAGTTATTCT CAAGCACCAT TTTCTCGCCC TCTTCGCTCT
              481 TGATCAGGGC GATATCCTCC TCGATGTCAG GCCACTCGTC CCAGGACTCG ATCACGTCCA
              541 CGACACTCTC AGCATGGACG ATGGCCTTGA TCTTGTCTTG GTGCTCGTAG GAGTAGTGAA
              601 AGGCCAGACA AGCCCCCCAG TCGTGGCCCA CAAAGATGAT TTTCTTTGGA AGGTTCAGCA
              661 GCTCGAACCA AGCGGTGAGG TACTTGTAGT GATCCAGGAG GCGATATGAG CCATTCCCGC
              721 TCTTGCCGGA CTTACCCATT CCGATCAGAT CAGGGATGAT GCATCTAGCC ACGGGCTCGA
              781 TGTGAGGCAC GACGTGCCTC CACAGGTAGC TGGAGGCAGC GTTACCATGC AGAAAAATCA
              841 CGGCGTTCTC GGCGTGCTTC TCGGAATCAT AGTAGTTGAT GAAGGAGTCC AGCACGTTCA
              901 TTTGCTTGCA GCGAGCCCAC CACTGAGGCC CAGTGATCAT GCGTTTGCGT TGCTCGGGGT
              961 CGTACACCTT GGAAGCCATG GGTGGCTAGC CTATAGTGAG TCGTATTAAG TACTCTAGCC
             1021 TTAAGAGCTG TAATTGAACT GGGGAGTGGA CACTGTGAGA GAAGGCAAGT GGATGTCAGT
             1081 TAGACATTAG GTGCTATCAG AAACGCAGAG TCTTCTCTGG TCTCGGACAG CCCAGTTCTA
            1141 TTGATCCTCC TTAAACCTGA TCCTT
            //

            Please visit Sequencing and PCR primers for primer information.

            References

            Original, user report and related articles

            2023.11.08

            GNP_filter3_RDBDEP_html_231027.pl