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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pSmad RE-TK hRluc(F)

Reporter construct of SMAD response element

Catalog number RDB05622
Resource name pSmad RE-TK hRluc(F)
Alternative name 2477C3F
Clone info. Smad response element is cloned into the BglII site of phRL-TK, and the BglII site is killed by the cloning. The nucleotide sequence of the Smad response element is 5'-gatccgtctagacgtctagacg-3'. (Ref: Mol Cell 1: 611-617, 1998)
Comment The nucleotide sequence of the element was determined. Function of the inserted element has not been examined by the depositor.
Vector backbone phRL-TK (Plasmid)
Size of vector backbone 4.0 kb
Selectable markers Amp^r
Gene/insert name Artificial Smad response element synthesized
Depositor|Developer Yokoyama, Kazunari |
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External Database
Artificial Smad response element

            Reference sequence
              
             
            Remarks, protocol and/or map (pdf) RDB05622.pdf

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions for distribution The RECIPIENT agrees to expressly describe the acknowledgement of the Gene Engineering Division, RIKEN BRC as the source of the BIOLOGICAL RESOURCE in any publication. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for-profit organization for a not-for-profit research.
            Additional terms and conditions:
            The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
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            RDB05622 pSmad RE-TK hRluc(F) DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            How to cite this biological resource

            Materials & Methods section:

            The pSmad RE-TK hRluc(F) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB05622).

            Reference section:

            Further references such as user reports and related articles (go to bottom)


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            QC test results

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB05622_A3Fqp1.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: SV40pA3'Element Reverse
            Sequence file: RDB05622_A3Fqa.seq check
            >05622_05622_A3Fq_SV40pA3'ElementReverse_F11_17.ab1
                1 GGGCAGGGGG GGGGCGGACC TATGGAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG
               61 CCTTTTGCTG GCCTTTTGCT CACATGGCTC GACAGATCCG TCTAGACGTC TAGACGGATC
              121 TAAATGAGTC TTCGGACCTC GCGGGGGCCG CTTAAGCGGT GGTTAGGGTT TGTCTGACGC
              181 GGGGGGAGGG GGAAGGAACG AAACACTCTC ATTCGGAGGC GGCTCGGGGT TTGGTCTTGG
              241 TGGCCACGGG CACGCAGAAG AGCGCCGCGA TCCTCTTAAG CACCCCCCCG CCCTCCGTGG
              301 AGGCGGGGGT TTGGTCGGCG GGTGGTAACT GGCGGGCCGC TGACTCGGGC GGGTCGCGCG
              361 CCCCAGAGTG TGACCTTTTC GGTCTGCTCG CAGACCCCCG GGCGGCGCCG CCGCGGCGGC
              421 GACGGGCTCG CTGGGTCCTA GGCTCCATGG GGACCGTATA CGTGGACAGG CTCTGGAGCA
              481 TCCGCACGAC TGCGGTGATA TTACCGGAGA CCTTCTGCGG GACGAGCCGG GTCACGCGGC
              541 TGACGCGGAG CGTCCGTTGG GCGACAAACA CCAGGACGGG GCACAGGTAC ACTATCTTGT
              601 CACCCGGAGG CGCGAGGGAC TGCAGGAGCT TCAGGGAGTG GCGCAGCTGC TTCATCCCCG
              661 TGGCCCGTTG CTCGCGTTTG CTGGCGGTGT CCCCGGAAGA AATATATTTG CATGTCTTTA
              721 GTTCTATGAT GACACAAACC CCGCCCAGCG TCTTGTCATT GGCGAATTCG AACACGCAGA
              781 TGCAGTCGGG GCGGCGCGGT CCCAGGTCCA CTTCGCATAT TAAGGTGACG CGTGTGGGCC
              841 TCGAACACCG AGCGACCCTG CAGCGACCCG CTTAAAAAGC TTGATTCTTC TGACACAACA
              901 GTCTCGAACT TAAGCTGCAG AAGTTGGGTC GTGAGGCACT GGGGCAGGTA AGTATCAAGG
              961 GTTTACAAAG ACAGGGTTTA AGGGAGGACC CAATAAGAAA CTGGGGCTTT GGTCGGAGAC
             1021 CAGGAAGAAG ACTCTTTGCC GTTTCCTGAT AGCAACCTAA TTGGGTCGTT AACTTGACCA
            1081 ATTC
            //
            Primer: pAxCALNL_F1
            Sequence file: RDB05622_A3Fqb.seq check
            >05622_05622_A3Fq_pAxCALNL_F1_G11_20.ab1
                1 AAAATTATCT TTATCATGTC TGCTCGAGCG GCCGCTCTAG AATTACTGCT CGTTCTTCAG
               61 CACGCGCTCC ACGAAGCTCT TGATGTACTT ACCCATTTCA TCTGGAGCGT CCTCCTGGCT
              121 GAAGTGGAGG CCCTTCACCT TCACGAACTC GGTGTTAGGG AACTTCTTAG CTCCCTCGAC
              181 AATAGCGTTG GAAAAGAACC CAGGGTCGGA CTCGATGAAC ATCTTAGGCA GATCGTCGCT
              241 GGCCCGAAGG TAGGCGTTGT AGTTGCGGAC AATCTGGACG ACGTCGGGCT TGCCTCCCTT
              301 AACGAGAGGG ATCTCGCGAG GCCAGGAGAG GGTAGGCCGT CTAACCTCGC CCTTCTCCTT
              361 GAATGGCTCC AGGTAGGCAG CGAACTCCTC AGGCTCCAGT TTCCGCATGA TCTTGCTTGG
              421 GAGCATGGTC TCGACGAAGA AGTTATTCTC AAGCACCATT TTCTCGCCCT CTTCGCTCTT
              481 GATCAGGGCG ATATCCTCCT CGATGTCAGG CCACTCGTCC CAGGACTCGA TCACGTCCAC
              541 GACACTCTCA GCATGGACGA TGGCCTTGAT CTTGTCTTGG TGCTCGTAGG AGTAGTGAAA
              601 GGCCAGACAA GCCCCCCAGT CGTGGCCCAC AAAGATGATT TTCTTTGGAA GGTTCAGCAG
              661 CTCGAACCAA GCGGTGAGGT ACTTGTAGTG ATCCAGGAGG CGATATGAGC CATTCCCGCT
              721 CTTGCCGGGA CTTACCCATT CCGATCAGAT CAGGGATGGA TGCATCTAGC CCACGGGCTT
            781 CGA
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles


            2023.11.08

            GNP_filter3_RDBDEP_html_231027.pl