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pSmad RE-TK hRluc(F)

Reporter construct of SMAD response element

Catalog number RDB05622
Resource name pSmad RE-TK hRluc(F)
Alternative name 2477C3F
Clone info. Smad response element is cloned into the BglII site of phRL-TK, and the BglII site is killed by the cloning. The nucleotide sequence of the Smad response element is 5'-gatccgtctagacgtctagacg-3'. (Ref: Mol Cell 1: 611-617, 1998)
The nucleotide sequence of the element was determined. Function of the inserted element has not been examined by the depositor.
Vector backbone phRL-TK (Plasmid)
Size of vector backbone 4.0 kb
Selectable markers Amp^r
Gene/insert name Artificial Smad response element synthesized
Depositor Yokoyama, Kazunari |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR The RECIPIENT agrees to expressly describe the acknowledgement of the Gene Engineering Division, RIKEN BRC as the source of the BIOLOGICAL RESOURCE in any publication. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for-profit organization for a not-for-profit research.
Additional terms and conditions for distribution The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB05622 pSmad RE-TK hRluc(F) DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file PDF RDB05622.pdf from Depositor

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References

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Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB05622_A3Fqp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: SV40pA3'Element Reverse RDB05622_A3Fqa.seq
>05622_05622_A3Fq_SV40pA3'ElementReverse_F11_17.ab1
    1 GGGCAGGGGG GGGGCGGACC TATGGAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG
   61 CCTTTTGCTG GCCTTTTGCT CACATGGCTC GACAGATCCG TCTAGACGTC TAGACGGATC
  121 TAAATGAGTC TTCGGACCTC GCGGGGGCCG CTTAAGCGGT GGTTAGGGTT TGTCTGACGC
  181 GGGGGGAGGG GGAAGGAACG AAACACTCTC ATTCGGAGGC GGCTCGGGGT TTGGTCTTGG
  241 TGGCCACGGG CACGCAGAAG AGCGCCGCGA TCCTCTTAAG CACCCCCCCG CCCTCCGTGG
  301 AGGCGGGGGT TTGGTCGGCG GGTGGTAACT GGCGGGCCGC TGACTCGGGC GGGTCGCGCG
  361 CCCCAGAGTG TGACCTTTTC GGTCTGCTCG CAGACCCCCG GGCGGCGCCG CCGCGGCGGC
  421 GACGGGCTCG CTGGGTCCTA GGCTCCATGG GGACCGTATA CGTGGACAGG CTCTGGAGCA
  481 TCCGCACGAC TGCGGTGATA TTACCGGAGA CCTTCTGCGG GACGAGCCGG GTCACGCGGC
  541 TGACGCGGAG CGTCCGTTGG GCGACAAACA CCAGGACGGG GCACAGGTAC ACTATCTTGT
  601 CACCCGGAGG CGCGAGGGAC TGCAGGAGCT TCAGGGAGTG GCGCAGCTGC TTCATCCCCG
  661 TGGCCCGTTG CTCGCGTTTG CTGGCGGTGT CCCCGGAAGA AATATATTTG CATGTCTTTA
  721 GTTCTATGAT GACACAAACC CCGCCCAGCG TCTTGTCATT GGCGAATTCG AACACGCAGA
  781 TGCAGTCGGG GCGGCGCGGT CCCAGGTCCA CTTCGCATAT TAAGGTGACG CGTGTGGGCC
  841 TCGAACACCG AGCGACCCTG CAGCGACCCG CTTAAAAAGC TTGATTCTTC TGACACAACA
  901 GTCTCGAACT TAAGCTGCAG AAGTTGGGTC GTGAGGCACT GGGGCAGGTA AGTATCAAGG
  961 GTTTACAAAG ACAGGGTTTA AGGGAGGACC CAATAAGAAA CTGGGGCTTT GGTCGGAGAC
 1021 CAGGAAGAAG ACTCTTTGCC GTTTCCTGAT AGCAACCTAA TTGGGTCGTT AACTTGACCA
1081 ATTC
//
Primer: pAxCALNL_F1 RDB05622_A3Fqb.seq
>05622_05622_A3Fq_pAxCALNL_F1_G11_20.ab1
    1 AAAATTATCT TTATCATGTC TGCTCGAGCG GCCGCTCTAG AATTACTGCT CGTTCTTCAG
   61 CACGCGCTCC ACGAAGCTCT TGATGTACTT ACCCATTTCA TCTGGAGCGT CCTCCTGGCT
  121 GAAGTGGAGG CCCTTCACCT TCACGAACTC GGTGTTAGGG AACTTCTTAG CTCCCTCGAC
  181 AATAGCGTTG GAAAAGAACC CAGGGTCGGA CTCGATGAAC ATCTTAGGCA GATCGTCGCT
  241 GGCCCGAAGG TAGGCGTTGT AGTTGCGGAC AATCTGGACG ACGTCGGGCT TGCCTCCCTT
  301 AACGAGAGGG ATCTCGCGAG GCCAGGAGAG GGTAGGCCGT CTAACCTCGC CCTTCTCCTT
  361 GAATGGCTCC AGGTAGGCAG CGAACTCCTC AGGCTCCAGT TTCCGCATGA TCTTGCTTGG
  421 GAGCATGGTC TCGACGAAGA AGTTATTCTC AAGCACCATT TTCTCGCCCT CTTCGCTCTT
  481 GATCAGGGCG ATATCCTCCT CGATGTCAGG CCACTCGTCC CAGGACTCGA TCACGTCCAC
  541 GACACTCTCA GCATGGACGA TGGCCTTGAT CTTGTCTTGG TGCTCGTAGG AGTAGTGAAA
  601 GGCCAGACAA GCCCCCCAGT CGTGGCCCAC AAAGATGATT TTCTTTGGAA GGTTCAGCAG
  661 CTCGAACCAA GCGGTGAGGT ACTTGTAGTG ATCCAGGAGG CGATATGAGC CATTCCCGCT
  721 CTTGCCGGGA CTTACCCATT CCGATCAGAT CAGGGATGGA TGCATCTAGC CCACGGGCTT
781 CGA
//

Please visit Sequencing and PCR primers for primer information.


References

References


2018.12.10

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