Please review the QC test results indicated by 
icon below as well as clone information before placing your order.
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Please review the QC test results indicated by icon below as well as clone information before placing your order.
icon below as well as clone information before placing your order.
pSmad RE-TK hRluc(F) (#RDB05622)
Reporter construct of SMAD response element
| Alternative name | 2477C3F |
|---|---|
| Clone info. | Smad response element is cloned into the BglII site of phRL-TK, and the BglII site is killed by the cloning. The nucleotide sequence of the Smad response element is 5'-gatccgtctagacgtctagacg-3'. (Ref: Mol Cell 1: 611-617, 1998) |
| Comment | The nucleotide sequence of the element was determined. Function of the inserted element has not been examined by the depositor. |
| Vector backbone | phRL-TK (Plasmid) |
| Size of vector backbone | 4.0 kb |
| Selectable markers | Amp^r |
| Gene/insert name | Artificial Smad response element synthesized |
| Depositor|Developer | Yokoyama, Kazunari | |
| Remarks, protocol and/or map (pdf) | RDB05622.pdf |
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Distribution information
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
| Ordering forms | Order form [Credit Card Payment  ] [Bank Transfer Payment] [Example of order form ]MTA, for use for not-for-profit academic purpose [Word ] [Example of MTA ]Please visit Information of Request for Distribution.[link] |
|---|---|
| Terms and conditions for distribution | The RECIPIENT agrees to expressly describe the acknowledgement of the Gene Engineering Division, RIKEN BRC as the source of the BIOLOGICAL RESOURCE in any publication. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for-profit organization for a not-for-profit research. Additional terms and conditions: The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation. |
[open/close]| 必要書類 | 提供依頼書 [依頼書の記入例 ]提供同意書 (MTA, 非営利学術目的用)[Word ] [MTAの記入例 ]手続きの概要は、「提供申込みについて[link]」をご覧ください。 |
|---|---|
| MTAに書く使用条件 | リソースを使用して論文を投稿する場合、理研BRC・遺伝子材料開発室から分譲された事を記載する。本件リソースは非営利機関にのみ提供し、非営利研究にのみ利用することができる。 MTAに書く付加的使用条件： 本件リソースの使用は学術機関での学術研究に限る。本件リソースの使用にあたって、利用者はプロメガ社の限定使用条件に従う必要がある。 |
| Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
|---|---|---|---|
| RDB05622 | pSmad RE-TK hRluc(F) | DNA solution |
icon below as well as clone information before placing your order.How to cite this biological resource
Materials & Methods section:
| The pSmad RE-TK hRluc(F) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB05622). |
Reference section:
Further references such as user reports and related articles (go to bottom)
QC test results
RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.
| Test sheet | RDB05622_A3Fqp1.pdf |
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Nucleotide sequence of a portion of this resource (if available).
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Primer: SV40pA3'Element Reverse Sequence file: RDB05622_A3Fqa.seq
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>05622_05622_A3Fq_SV40pA3'ElementReverse_F11_17.ab1 1 GGGCAGGGGG GGGGCGGACC TATGGAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG 61 CCTTTTGCTG GCCTTTTGCT CACATGGCTC GACAGATCCG TCTAGACGTC TAGACGGATC 121 TAAATGAGTC TTCGGACCTC GCGGGGGCCG CTTAAGCGGT GGTTAGGGTT TGTCTGACGC 181 GGGGGGAGGG GGAAGGAACG AAACACTCTC ATTCGGAGGC GGCTCGGGGT TTGGTCTTGG 241 TGGCCACGGG CACGCAGAAG AGCGCCGCGA TCCTCTTAAG CACCCCCCCG CCCTCCGTGG 301 AGGCGGGGGT TTGGTCGGCG GGTGGTAACT GGCGGGCCGC TGACTCGGGC GGGTCGCGCG 361 CCCCAGAGTG TGACCTTTTC GGTCTGCTCG CAGACCCCCG GGCGGCGCCG CCGCGGCGGC 421 GACGGGCTCG CTGGGTCCTA GGCTCCATGG GGACCGTATA CGTGGACAGG CTCTGGAGCA 481 TCCGCACGAC TGCGGTGATA TTACCGGAGA CCTTCTGCGG GACGAGCCGG GTCACGCGGC 541 TGACGCGGAG CGTCCGTTGG GCGACAAACA CCAGGACGGG GCACAGGTAC ACTATCTTGT 601 CACCCGGAGG CGCGAGGGAC TGCAGGAGCT TCAGGGAGTG GCGCAGCTGC TTCATCCCCG 661 TGGCCCGTTG CTCGCGTTTG CTGGCGGTGT CCCCGGAAGA AATATATTTG CATGTCTTTA 721 GTTCTATGAT GACACAAACC CCGCCCAGCG TCTTGTCATT GGCGAATTCG AACACGCAGA 781 TGCAGTCGGG GCGGCGCGGT CCCAGGTCCA CTTCGCATAT TAAGGTGACG CGTGTGGGCC 841 TCGAACACCG AGCGACCCTG CAGCGACCCG CTTAAAAAGC TTGATTCTTC TGACACAACA 901 GTCTCGAACT TAAGCTGCAG AAGTTGGGTC GTGAGGCACT GGGGCAGGTA AGTATCAAGG 961 GTTTACAAAG ACAGGGTTTA AGGGAGGACC CAATAAGAAA CTGGGGCTTT GGTCGGAGAC 1021 CAGGAAGAAG ACTCTTTGCC GTTTCCTGAT AGCAACCTAA TTGGGTCGTT AACTTGACCA 1081 ATTC // |
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Primer: pAxCALNL_F1 Sequence file: RDB05622_A3Fqb.seq
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>05622_05622_A3Fq_pAxCALNL_F1_G11_20.ab1 1 AAAATTATCT TTATCATGTC TGCTCGAGCG GCCGCTCTAG AATTACTGCT CGTTCTTCAG 61 CACGCGCTCC ACGAAGCTCT TGATGTACTT ACCCATTTCA TCTGGAGCGT CCTCCTGGCT 121 GAAGTGGAGG CCCTTCACCT TCACGAACTC GGTGTTAGGG AACTTCTTAG CTCCCTCGAC 181 AATAGCGTTG GAAAAGAACC CAGGGTCGGA CTCGATGAAC ATCTTAGGCA GATCGTCGCT 241 GGCCCGAAGG TAGGCGTTGT AGTTGCGGAC AATCTGGACG ACGTCGGGCT TGCCTCCCTT 301 AACGAGAGGG ATCTCGCGAG GCCAGGAGAG GGTAGGCCGT CTAACCTCGC CCTTCTCCTT 361 GAATGGCTCC AGGTAGGCAG CGAACTCCTC AGGCTCCAGT TTCCGCATGA TCTTGCTTGG 421 GAGCATGGTC TCGACGAAGA AGTTATTCTC AAGCACCATT TTCTCGCCCT CTTCGCTCTT 481 GATCAGGGCG ATATCCTCCT CGATGTCAGG CCACTCGTCC CAGGACTCGA TCACGTCCAC 541 GACACTCTCA GCATGGACGA TGGCCTTGAT CTTGTCTTGG TGCTCGTAGG AGTAGTGAAA 601 GGCCAGACAA GCCCCCCAGT CGTGGCCCAC AAAGATGATT TTCTTTGGAA GGTTCAGCAG 661 CTCGAACCAA GCGGTGAGGT ACTTGTAGTG ATCCAGGAGG CGATATGAGC CATTCCCGCT 721 CTTGCCGGGA CTTACCCATT CCGATCAGAT CAGGGATGGA TGCATCTAGC CCACGGGCTT 781 CGA // |
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Sequencing and PCR primers for primer information.
References
Original, user report and related articles
Resource search
Link
Other resources in our bankExternal Database
Artificial Smad response element
Reference sequence
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