Resource data sheet
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pNF-kappaB RE-TK hRluc(F)

Reporter construct of NF-kB response element

Catalog number RDB05576
Resource name pNF-kappaB RE-TK hRluc(F)
Alternative name 2427C6F
Clone info. NF-kB response element is cloned into the BglII site of phRL-TK, and the BglII site is killed by the cloning. The nucleotide sequence of the NF-kB response element is 5'-gatccgggactttccgggactttccg-3'. (Ref: Cell 46: 705-716, 1986)
Comment The nucleotide sequence of the element was determined. Function of the inserted element has not been examined by the depositor.
Vector backbone phRL-TK (Plasmid)
Size of vector backbone 4.0 kb
Selectable markers Amp^r
Gene/insert name Artificial NF-kappaB response element synthesized
Depositor|Developer Yokoyama, Kazunari |
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External Database
Artificial NF-kappaB response element

            Reference sequence
            Remarks, protocol and/or map (pdf) RDB05576.pdf

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions for distribution The RECIPIENT agrees to expressly describe the acknowledgement of the Gene Engineering Division, RIKEN BRC as the source of the BIOLOGICAL RESOURCE in any publication. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for-profit organization for a not-for-profit research.
            Additional terms and conditions:
            The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
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            提供条件 リソースを使用して論文を投稿する場合、理研BRC・遺伝子材料開発室から分譲された事を記載する。本件リソースは非営利機関にのみ提供し、非営利研究にのみ利用することができる。
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            RDB05576 pNF-kappaB RE-TK hRluc(F) Under QC test. Please contact us. DNA solution

            check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

            How to cite this biological resource

            Materials & Methods section:

            The pNF-kappaB RE-TK hRluc(F) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB05576).

            Reference section:

            Further references such as user reports and related articles (go to bottom)


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            QC test results

            check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.


            Original, user report and related articles

            user_report Yamaguchi, J., Inflammation and hypoxia linked to renal injury by CCAAT/enhancer-binding protein delta. Kidney Int. 88 (2): 262-275 (2015). PMID 25692954. [link to RRC of NBRP]
            user_report Yamada, H., Gentiolactone, a secoiridoid dilactone from Gentiana triflora, inhibits TNF-alpha, iNOS and Cox-2 mRNA expression and blocks NF-kappaB promoter activity in murine macrophages. PLoS One 9 (11): e113834 (2014). PMID 25423092. [link to RRC of NBRP]
            user_report Shimizu, H., NF-kappaB plays an important role in indoxyl sulfate-induced cellular senescence, fibrotic gene expression, and inhibition of proliferation in proximal tubular cells. Am. J. Physiol. Cell Physiol. 301 (5): C1201-12 (2011). PMID 21832251. [link to RRC of NBRP]