Resource data sheet
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CSII-CMV-Venus

Expression vector of Venus GFP driven by CMV promoter

Catalog number RDB05553
Resource name CSII-CMV-Venus
Clone info. Expression vector of Venus GFP driven by CMV promoter.
Commonly requested with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394).
Vector backbone Plasmid
Selectable markers Amp^r
Gene/insert name Aequorea victoria GFP cDNA
Originator Miyoshi, Hiroyuki |

Distribution information

CLICK here for plasmid list and map. [link]
Forms for plasmid request [in Japanese] [in English]
Terms and conditions for distribution The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose.
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Remarks ((Remarks)) Remember that you will be working with samples containing infectious virus.
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Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB12356_A4Afp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CSII-CMV5' RDB12356_A4Afa.seq
>05553_12356_A4Af_CSII-CMV5'_A02_02.ab1
    1 ACGGCAACGC TCGGTCTCGA GATTCTGCAG CGGCCGCGGA TCCACCATGG TGAGCAAGGG
   61 CGAGGAGCTG TTCACCGGGG TGGTGCCCAT CCTGGTCGAG CTGGACGGCG ACGTAAACGG
  121 CCACAAGTTC AGCGTGTCCG GCGAGGGCGA GGGCGATGCC ACCTACGGCA AGCTGACCCT
  181 GAAGCTGATC TGCACCACCG GCAAGCTGCC CGTGCCCTGG CCCACCCTCG TGACCACCCT
  241 GGGCTACGGC CTGCAGTGCT TCGCCCGCTA CCCCGACCAC ATGAAGCAGC ACGACTTCTT
  301 CAAGTCCGCC ATGCCCGAAG GCTACGTCCA GGAGCGCACC ATCTTCTTCA AGGACGACGG
  361 CAACTACAAG ACCCGCGCCG AGGTGAAGTT CGAGGGCGAC ACCCTGGTGA ACCGCATCGA
  421 GCTGAAGGGC ATCGACTTCA AGGAGGACGG CAACATCCTG GGGCACAAGC TGGAGTACAA
  481 CTACAACAGC CACAACGTCT ATATCACCGC CGACAAGCAG AAGAACGGCA TCAAGGCCAA
  541 CTTCAAGATC CGCCACAACA TCGAGGACGG CGGCGTGCAG CTCGCCGACC ACTACCAGCA
  601 GAACACCCCC ATCGGCGACG GCCCCGTGCT GCTGCCCGAC AACCACTACC TGAGCTACCA
  661 GTCCGCCCTG AGCAAAGACC CCAACGAGAA GCGCGATCAC ATGGTCCTGC TGGAGTTCGT
  721 GACCGCCGCC GGGATCACTC TCGGCATGGA CGAGCTGTAC AAGTAAGAAT TCAAGGCCTC
  781 TCGAGCCTCT AGAGTTAACA TCGAGGGATC AAGCTTATCG ATAATCAACC TCTGGATTAC
  841 AAAATTTGTG AAAGATTGAC TGGTATTCTT AACTATGTTG CTCCTTTTAC GCTATGTGGA
  901 TACGCTGCTT TAATGCCTTT GTATCATGCT ATTGCTTCCC GTATGGCTTT CATTTTCTCC
  961 TCCTTGTATA AATCCTGGTT GCTGTCTCTT TATGAGGAGT TGTGGCCCGT TGTCAGCAAC
 1021 GTGGCGTGTG TGCACTGTGT TTGCTGACGC ACCCCCACTG GTTGGGGGCA TTGCACACTG
 1081 TCAGCTCCTT TTCCAGACTT TCGCTTTCCC CCACCTATTG CCACGGCGGA AACTTCATTC
1141 CGCCCGCCCT GTG
//
Primer: HIVgp_R1 RDB12356_A4Afb.seq
>05553_12356_A4Af_HIVgp_R1_B02_05.ab1
    1 CCAGCTCTAT TCCACTGCTC TTTTTTCTCT CTGCACCACT CTTCTCTTTG CCTTGGTGGG
   61 TGCTACTCCT AATGGTTCAA TTTTTACTAC TTTATATTTA TATAATTCAC TTCTCCAATT
  121 GTCCCTCATA TCTCCTCCTC CAGGTCTGAA GATCAGCGGC CGGCCGCTTG CTGTGCGGTG
  181 GTCTTACTTT TGTTTTGCTC TTCCTCTATC TTGTCTAAAG CTTCCTTGGT GTCTTTTATC
  241 TCTATCCTTT GATGCACACA ATAGAGGGTT GCTACTGTAT TATATAATGA TCTAAGTTCT
  301 TCTGATCCTG TCTGAAGGGA TGGTTGTAGC TGTCCCAGTA TTTGTCTACA GCCTTCTGAT
  361 GTTTCTAACA GGCCAGGATT AACTGCGAAT CGTTCTAGCT CCCTGCTTGC CCATACTATA
  421 TGTTTTAATT TATATTTTTT CTTTCCCCCT GGCCTTAACC GAATTTTTTC CCATCGCGAT
  481 CTAATTCTCC CCCGCTTAAT ACTGACGCTC TCGCACCCAT CTCTCTCCTT CTAGCCTCCG
  541 CTAGTCAAAA TTTTTGGCGT ACTCACCAGT CGCCGCCCCT CGCCTCTTGC CGTGCGCGCT
  601 TCAGCAAGCC GAGTCCTGCG TCGAGAGAGC TCCTCTGGTT TCCCTTTCGC TTTCAAGTCC
  661 CTGTTCGGGC GCCACTGCTA GAGATTTTCC ACACTGACTA AAAGGGTCTG AGGGATCTCT
  721 AGTTACCAGA GTCACACAAC AGACGGGCAC ACACTACTTG AAGCACTCAA GGCAAGCTTT
  781 ATTGAGGCTT AAGCAGTGGG TTCCCTAGTT AGCCAGAGAG CTCCCAGGCT CAGATCTGGT
  841 CTAACCAGAG AGACCCAGTA CAGGCAAAAC GCGCTGCTTA TATAGACCTC CCACCGTACA
  901 CGCCTACCGC CCATTTGCGT CAATGGGGCG GAGTTGTTAC GACATTTTGG AAAGTCCCGT
  961 TGATTTTGGT GCCAAAACAA ACTCCCATTG ACGTCAATGG GGTGGAGACT TGGAAATCCC
 1021 CGTGAGTCAA CCGCTATCCA CGCCCATTGA TGTACTGCCA AAACCGCATC ACCATGGTAA
 1081 TAGCGATGAC TAATACGTAG ATGTACTGCC AAGTAAGAAG TCCATACGTT CAATGTACTG
1141 GGCATAATGC CAGGCGGGGC CCAATTTTAA CCCGGTTCCA TTTGAA
//
Primer: BGH_rev RDB12356_A4Afc.seq
>05553_12356_A4Af_BGHrev_C02_08.ab1
    1 GTTACGGGTC TGCTAGAGAT TTTCCACACT GACTAAAAGG GTCTGAGGGA TCTCTAGTTA
   61 CCAGAGTCAC ACAACAGACG GGCACACACT ACTTGAAGCA CTCAAGGCAA GCTTTATTGA
  121 GGCTTAAGCA GTGGGTTCCC TAGTTAGCCA GAGAGCTCCC AGGCTCAGAT CTGGTCTAAC
  181 CAGAGAGACC CAGTACAGTC CGGATGCAGC TCTCGGGCCA TGTGATGAAA TGCTAGGCGG
  241 CTGTCAAACC TCCACTCTAA TACTTCTCTC TCCGGGTCAT CCATCCCATG CAGGCTCACA
  301 GGGTGTAACA AGCGGGTGTT CTCTCCTTCA TTGGCTTCTT CTACCTTCTC TTGCTCAACT
  361 GGTACTAGCT TGTAGCACCA TCCAAAGGTC AGTGGATATC TGATCCCTGG CCCTGGTGTG
  421 TAGTTCTGCC AATCAGGGAA GTAGCCTTGT GTGTGGTAGA TCCACAGATC AAGGATATCT
  481 TGTCTTCGTT GGGAGTGAAT TAGCCCTTCC AGTCCCCCCT TTTCTTTTAA AAAGTGGCTA
  541 AGATCTACAG CTGCCTTGTA AGTCATTGGT CTTAAAGGTA CCTGAGGTGT GACTGGAAAA
  601 CCCACCTCCT CCTCCTCTTG TGCTTCTAGC CAGGCACAAT CAGCATTGGT AGCTGCTGTA
  661 TTGCTACTTG TGATTGCTCC ATGTTTTTCT AGGTCTCGAC GGTATCGATG CGGGGAGGCG
  721 GCCCAAAGGG AGATCCGACT CGTCTGAGGG CGAAGGCGAA GACGCGGAAG AGGCCGCAGA
  781 GCCGGCAGCA GGCCGCGGGA AGGAAGGTCC GCTGGATTGA GGGCCGAAGG GACGTAGCAG
  841 AAGGACGTCC CGCGCAGAAT CCAGGTGGCA ACACAGGCGA GCAGCCATGG AAAGGACGTC
  901 AGCTTCCCCG ACAACACCAC GGAATTGTCA GTGCCCAACA GCCGAGCCTC CTGTCCAGCA
  961 GCGGGCAAAG GCAGGCGGCG ATGAGTTCCG CCGTGGCAAT AGGGAGGGGG AAAGCGAAAG
 1021 TCCTCGGGAA GGGAGCTGAC AGGTGGTGGC AATGCCTCCA ACTCAGTGGG GGGGTTGCGT
 1081 CAGCAAACAC AGTGCACCAT CACGTCACGT TGCCTGACAC GGGCCACACT TCTCATAAGA
 1141 AGACAGCCAC CAGGAATTTA TACACGGGAG GGGAGGAAAA ATTGAAAGGT CTCAATATAC
1201 CG
//
Primer: SV40pro_F RDB12356_A4Afd.seq
>05553_12356_A4Af_SV40pro_F_D02_11.ab1
    1 CCCTGGTCGT GGCATTCAGA GTAGTGAGGA GGCTTTTTTG GAGGCCTAGG CTTTTGCAAA
   61 AAGCTCCCGG GAGCTTGTAT ATCCATTTTC GGATCTGATC AGCACGTGTT GACAATTAAT
  121 CATCGGCATA GTATATCGGC ATAGTATAAT ACGACAAGGT GAGGAACTAA ACCATGGCCA
  181 AGTTGACCAG TGCCGTTCCG GTGCTCACCG CGCGCGACGT CGCCGGAGCG GTCGAGTTCT
  241 GGACCGACCG GCTCGGGTTC TCCCGGGACT TCGTGGAGGA CGACTTCGCC GGTGTGGTCC
  301 GGGACGACGT GACCCTGTTC ATCAGCGCGG TCCAGGACCA GGTGGTGCCG GACAACACCC
  361 TGGCCTGGGT GTGGGTGCGC GGCCTGGACG AGCTGTACGC CGAGTGGTCG GAGGTCGTGT
  421 CCACGAACTT CCGGGACGCC TCCGGGCCGG CCATGACCGA GATCGGCGAG CAGCCGTGGG
  481 GGCGGGAGTT CGCCCTGCGC GACCCGGCCG GCAACTGCGT GCACTTCGTG GCCGAGGAGC
  541 AGGACTGACA CGTGCTACGA GATTTCGATT CCACCGCCGC CTTCTATGAA AGGTTGGGCT
  601 TCGGAATCGT TTTCCGGGAC GCCGGCTGGA TGATCCTCCA GCGCGGGGAT CTCATGCTGG
  661 AGTTCTTCGC CCACCCCAAC TTGTTTATTG CAGCTTATAA TGGTTACAAA TAAAGCAATA
  721 GCATCACAAA TTTCACAAAT AAAGCATTTT TTTCACTGCA TTCTAGTTGT GGTTTGTCCA
  781 AACTCATCAA TGTATCTTAT CATGTCTGTA TACCGTCGAC CTCTAGCTAG AGCTTGGCGT
  841 AATCATGGTC ATAGCTGTTT CCTGTGTGAA ATTGTTATCC GCTCACAATT CCACACAACA
  901 TACGAGCCGG AAGCATAAAG TGTAAAGCCT GGGGTGCCTA ATGAGTGAGC TAACTCACAT
  961 TAATTGCGTT GCGCTCACTG CCCGCTTTCC AGTCGGGAAA CCTGTCGTGC CAGCTGCATT
 1021 AATGAATCGG GCCAACGCGC GGGGGAGAGG CGGGTTTGCG TATTGGGGCG CTCTTTCCGC
 1081 CTTCTCGCTC ACTGACTCGC TGCGCTCGCT CGCTCCGCCT GCCGGCCGAG CCGCTATCAG
1141 CCTCACTCCA AAGGCCGGTA ATTACCCGGC TATC
//

Please visit plasmidSequencing and PCR primers for primer information.

References and tips

Electronic file

References

user report Mu, P., A novel nuclear complex of DRR1, F-actin and COMMD1 involved in NF-κB degradation and cell growth suppression in neuroblastoma. Oncogene. 2017 Jun 12. doi: 10.1038/onc.2017.181. [Epub ahead of print] PMID 28604741.
user report Murakami-Tonami, Y., SGO1 is involved in the DNA damage response in MYCN-amplified neuroblastoma cells. Sci. Rep. 6: 31615 (2016). PMID 27539729.
user report Nakaguro, M., Nucleolar protein PES1 is a marker of neuroblastoma outcome and is associated with neuroblastoma differentiation. Cancer Sci. 106 (3): 237-243 (2015). PMID 25557119.
user report Murakami-Tonami, Y., Inactivation of SMC2 shows a synergistic lethal response in MYCN-amplified neuroblastoma cells. Cell Cycle 13 (7): 1115-1131 (2014). PMID 24553121.
user report Muraoka, N., MiR-133 promotes cardiac reprogramming by directly repressing Snai1 and silencing fibroblast signatures. EMBO J., 33 (14): 1565-1581 (2014). PMID 24920580.
user report Huang, P., The neuronal differentiation factor NeuroD1 downregulates the neuronal repellent factor Slit2 expression and promotes cell motility and tumor formation of neuroblastoma. Cancer Res., 71 (8), 2938-2948 (2011). PMID 21349947.

2017.10.23

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