Please review the QC test results indicated by icon below as well as clone information before placing your order.

CSII-EF-Venus

Lentivirus vector plasmid of Venus fluorescent protein driven by EF-1 alpha promoter.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer. Additional terms and conditions: RECIPIENT is requested to obtain written consent of Dr. Atsushi Miyawaki of the RIKEN CBS regarding Venus fluorescent protein.
Ordering	Please visit Ordering instruction.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA, for use for not-for-profit academic purpose)[Word] Approval form [Word] by Dr. Atsushi Miyawaki
Remarks	((Remarks)) Remember that you will be working with samples containing infectious virus. Please contact us. We will provide you with appropriate documents. Please obtain written approval of Dr. Atsushi Miyawaki.

提供案内 (日本国内) [open/close]

提供条件	本件研究材料は、非営利機関の非営利学術研究に限って提供する。本件研究材料を利用した研究結果等を発表する際は、本件研究材料が故三好浩之博士により開発されたことを明示する。 付加的提供条件： 利用者は、Venus蛍光タンパク質について理化学研究所CBS 宮脇敦史博士から、書面による提供承諾を得ること。
提供依頼	手続きの詳細は、「レンチウイルスベクターの提供申し込み」をご覧ください。 提供依頼書 [Word] 提供同意書 (非営利機関による非営利学術研究用)[Word] 遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。 宮脇敦史博士による提供承諾書 [Word]
備考	【使用上の注意】このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。 宮脇敦史博士の書面による提供承諾が必要です。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

Reference section:

Further references such as user reports and related articles (go to bottom)

References and tips

Featured content

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB18578_B0I9p1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: EF1a-C_F (Pr0168) Region: EF-1 alpha pro,Venus 5' Sequence file: RDB18578_B0I9a.seq
>05552_18578_B0I9_2_EF1a-C_F_A04_01_ABI24.ab1     1 NNNNNNNNNN NNNNNNNTTT NANTTNGGNN CTTGGTTNNT TCTCNAGCCT CAGACAGTGG    61 TTCAAAGTTT TTTTCTTCCA TTTCAGGTGT CGTGAACACG CTACCGGTCT CGAGAATTCT   121 GCAGCGGCCG CGGATCCACC ATGGTGAGCA AGGGCGAGGA GCTGTTCACC GGGGTGGTGC   181 CCATCCTGGT CGAGCTGGAC GGCGACGTAA ACGGCCACAA GTTCAGCGTG TCCGGCGAGG   241 GCGAGGGCGA TGCCACCTAC GGCAAGCTGA CCCTGAAGCT GATCTGCACC ACCGGCAAGC   301 TGCCCGTGCC CTGGCCCACC CTCGTGACCA CCCTGGGCTA CGGCCTGCAG TGCTTCGCCC   361 GCTACCCCGA CCACATGAAG CAGCACGACT TCTTCAAGTC CGCCATGCCC GAAGGCTACG   421 TCCAGGAGCG CACCATCTTC TTCAAGGACG ACGGCAACTA CAAGACCCGC GCCGAGGTGA   481 AGTTCGAGGG CGACACCCTG GTGAACCGCA TCGAGCTGAA GGGCATCGAC TTCAAGGAGG   541 ACGGCAACAT CCTGGGGCAC AAGCTGGAGT ACAACTACAA CAGCCACAAC GTCTATATCA   601 CCGCCGACAA GCAGAAGAAC GGCATCAAGG CCAACTTCAA GATCCGCCAC AACATCGAGG   661 ACGGCGGCGT GCAGCTCGCC GACCACTACC AGCAGAACAC CCCCATCGGC GACGGCCCCG   721 TGCTGCTGCC CGACAACCAC TACCTGAGCT ACCAGTCCGC CCTGAGCAAA GACCCCAACG   781 AGAAGCGCGA TCACATGGTC CTGCTGGAGT TCGTGACCGC CGCCGGGATC ACTCTCGGCA   841 TGGACGANCT GTACAAGTAA GAATTCAAGG CCTCTCNAGC CTCTAGAGTT AACATCGAGG   901 GATCNAGCTT ATCGATAATC AACCNCTGGA TTACAAANTT TGTGAAAGAN TGACTGGNAT   961 TNNTAACTNN GTTGNNNCNT TTANNCTATG NGGANANNCN GNTTTAANGN CNNNNNNATC 1021 ANGNNNNNGN NTNNCNNNNN NNNNN //
Primer: PRE_rev (Pr0250) Region: PRE,Venus 3' Sequence file: RDB18578_B0I9b.seq
>05552_18578_B0I9_2_PRE_rev_B04_04_ABI24.ab1     1 NNNNNNNNNN NNNNNNGNNN NNNNANCNAN NTAGTTAAGA ATACCNGTCA ATCTTTCNCA    61 AATTTTGTAA TCCAGAGGTT GATTATCGAT AAGCTTGATC CCTCGATGTT AACTCTAGAG   121 GCTCGAGAGG CCTTGAATTC TTACTTGTAC AGCTCGTCCA TGCCGAGAGT GATCCCGGCG   181 GCGGTCACGA ACTCCAGCAG GACCATGTGA TCGCGCTTCT CGTTGGGGTC TTTGCTCAGG   241 GCGGACTGGT AGCTCAGGTA GTGGTTGTCG GGCAGCAGCA CGGGGCCGTC GCCGATGGGG   301 GTGTTCTGCT GGTAGTGGTC GGCGAGCTGC ACGCCGCCGT CCTCGATGTT GTGGCGGATC   361 TTGAAGTTGG CCTTGATGCC GTTCTTCTGC TTGTCGGCGG TGATATAGAC GTTGTGGCTG   421 TTGTAGTTGT ACTCCAGCTT GTGCCCCAGG ATGTTGCCGT CCTCCTTGAA GTCGATGCCC   481 TTCAGCTCGA TGCGGTTCAC CAGGGTGTCG CCCTCGAACT TCACCTCGGC GCGGGTCTTG   541 TAGTTGCCGT CGTCCTTGAA GAAGATGGTG CGCTCCTGGA CGTAGCCTTC GGGCATGGCG   601 GACTTGAAGA AGTCGTGCTG CTTCATGTGG TCGGGGTAGC GGGCGAAGCA CTGCAGGCCG   661 TAGCCCAGGG TGGTCACGAG GGTGGGCCAG GGCACGGGCA GCTTGCCGGT GGTGCAGATC   721 AGCTTCAGGG TCAGCTTGCC GTANGTGGCA TCGCCCTCGC CCTCGCCGGA CACGCTGAAC   781 TTGTGGCCGT TTACGTCGCC GTCCAGCTCG ACNAGGATGG GCACCACCCC GGNGAACAGC   841 TCCTCGCCCT TGCTCACCAT GGTGGATNNN CGGNCGCTGC AGAATTCTCG AGNNCGGTAN   901 CGNGTNNACG ACACCNNAAA TGGAAGAAAA AAAACTTTGA NNNNCNNNCT GANGNTTGAG   961 ANTGNACNNA NNNNNNNAAC TNAAAAAGGG NNAATTNNNN NNANNNNTAC NTNNNNNNNN 1021 NN //
Primer: CMV_Forward (Pr0016) Region: CMV pro,5' LTR,HIV-1 psi Sequence file: RDB18578_B0I9c.seq
>05552_18578_B0I9_2_CMV_Forward_C04_07_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNT GNNNCANTGG GCGGNAGGCG TGTACGGTGG GAGGTCTATA    61 TAAGCAGCGC GTTTTGCCTG TACTGGGTCT CTCTGGTTAG ACCAGATCTG AGCCTGGGAG   121 CTCTCTGGCT AACTAGGGAA CCCACTGCTT AAGCCTCAAT AAAGCTTGCC TTGAGTGCTT   181 CAAGTAGTGT GTGCCCGTCT GTTGTGTGAC TCTGGTAACT AGAGATCCCT CAGACCCTTT   241 TAGTCAGTGT GGAAAATCTC TAGCAGTGGC GCCCGAACAG GGACTTGAAA GCGAAAGGGA   301 AACCAGAGGA GCTCTCTCGA CGCAGGACTC GGCTTGCTGA AGCGCGCACG GCAAGAGGCG   361 AGGGGCGGCG ACTGGTGAGT ACGCCAAAAA TTTTGACTAG CGGAGGCTAG AAGGANNNAG   421 ATGGGTGCGA GAGCGTCAGT ATTAAGCGGG GGANAATTAG NTCGCGATGG GAAAAANTTC   481 GGNTNANGNC NGGGGGAAAN AAAAAATNTN ANTTNAANCN TNTNNTANGG GNANNCNNGG 541 ANCTNNANNN ATTCNNANTN NNNNCNNGNN NNNNNNNN //
Primer: cPPT/CTS-E_R (Pr0725) Region: cPPT_CTS,RRE Sequence file: RDB18578_B0I9d.seq
>05552_18578_B0I9_2_cPPT+CTS-E_R_D04_10_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNTTCTT NAGTTTGTAT GTCTGTTGCT ATTATGTCTA    61 CTATTCTTTC CCCTGCACTG TACCCCCCAA TCCCCCCTTT TCTTTTAAAA TTGTGGATGA   121 ATACTGCCAT TTGTAGATCC GTTCACTAAT CGAATGGATC TGTCTCTGTC TCTCTCTCCA   181 CCTTCTTCTT CTATTCCTTC GGGCCTGTCG GGTCCCCTCG GGGTTGGGAG GTGGGTCTGA   241 AACGATAATG GTGAATATCC CTGCCTAACT CTATTCACTA TAGAAAGTAC AGCAAAAACT   301 ATTCTTAAAC CTACCAAGCC TCCTACTATC ATTATGAATA ATTTTATATA CCACAGCCAA   361 TTTGTTATGT TAAACCAATT CCACAAACTT GCCCATTTAT CTAATTCCAA TAATTCTTGT   421 TCATTCTTTT CTTGCTGGTT TTGCGATTCT TCAATTAAGG AGTGTATTAA GCTTGTGTAA   481 TTGTTAATTT CTCTGTCCCA CTCCATCCAG GTCGTGTGAT TCCAAATCTG TTCCAGAGAT   541 TTATTACTCC AACTAGCATT CCAAGGCACA GCAGTGGTGC AAATGAGTTT TCCAGAGCAA   601 CCCCAAATCC CCAGGAGCTG TTGATCCTTT AGGTATCTTT CCACAGCCAG GATTCTTGCC   661 TGGAGCTGCT TGATGCCCCA GACTGTGAGT TGCAACAGAT GCTGTTGCGC CTCAATAGCC   721 CTCAGCAAAT TGTTCTGCTG CTGCACTATA CCAGACAATA ATTGTCTGGC CTGTACCGTC   781 AGCGTCATTG ACGCTGCGCC CATAGTGCTT CCTGCTGCTC CCAAGAANCC ANNGAACAAA   841 GCTCCTATTC CCACNGCTCT TTTTTCTCTN TGCNNCNACT CTTCTCTTTN CCNTGGTGGG   901 NGCTACTCCT AANGGTTCAN NTTTTNANNA CTTTATATTT ATATAANTCA CTTCTNCNAN   961 NNGTCCCTCA NATCTNCNTC NNCCANGNCT GNNNATNANN NGNCCCTTGC TGNGNNGNGN 1021 NNNTNNNTTT TNNTNNNNNN TNNNNNNNNN N //
Primer: bGH_rev3 (Pr0885) Region: bGHpA,3' LTR,PRE Sequence file: RDB18578_B0I9e.seq
>05552_18578_B0I9_2_bGH_rev3_E04_13_ABI24.ab1     1 NNNNNNNNNN NNNNANNNNN NNNGGGNNGG GNNNANNNCA GATGGCTGGC AACTAGAAGG    61 CACAGTCGAG GCTGATCAGC GGGTTTAAAC GGGCCCTGCT AGAGATTTTC CACACTGACT   121 AAAAGGGTCT GAGGGATCTC TAGTTACCAG AGTCACACAA CAGACGGGCA CACACTACTT   181 GAAGCACTCA AGGCAAGCTT TATTGAGGCT TAAGCAGTGG GTTCCCTAGT TAGCCAGAGA   241 GCTCCCAGGC TCAGATCTGG TCTAACCAGA GAGACCCAGT ACAGTCCGGA TGCAGCTCTC   301 GGGCCATGTG ATGAAATGCT AGGCGGCTGT CAAACCTCCA CTCTAATACT TCTCTCTCCG   361 GGTCATCCAT CCCATGCAGG CTCACAGGGT GTAACAAGCG GGTGTTCTCT CCTTCATTGG   421 CTTCTTCTAC CTTCTCTTGC TCAACTGGTA CTAGCTTGTA GCACCATCCA AAGGTCAGTG   481 GATATCTGAT CCCTGGCCCT GGTGTGTAGT TCTGCCAATC AGGGAAGTAG CCTTGTGTGT   541 GGTAGATCCA CAGATCAAGG ATATCTTGTC TTCGTTGGGA GTGAATTAGC CCTTCCAGTC   601 CCCCCTTTTC TTTTAAAAAG TGGCTAAGAT CTACAGCTGC CTTGTAAGTC ATTGGTCTTA   661 AAGGTACCTG AGGTGTGACT GGAAAACCCA CCTCCTCCTC CTCTTGTGCT TCTAGCCAGG   721 CACAATCAGC ATTGGTAGCT GCTGTATTGC TACTTGTGAT TGCTCCATGT TTTTCTAGGT   781 CTCGACGGTA TCGATGCGGG GANGCGGCCC AAAGGGANNT CCGACTCGTC TGANGGCGAA   841 NNCGAANANG CGGAAGANGC CNCANANNCN GCANCANGNN NCGGGAAGGA ANGNNCGCTN 901 GATTGAGGGN CGAANGGNNN TNNNNNNNNN N //
Primer: SV40pro_ori_F (Pr0731) Region: SV40 pro_ori,Zeo Sequence file: RDB18578_B0I9f.seq
>05552_18578_B0I9_2_SV40pro_ori_F_F04_16_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNC NNCCTCTGCC TCTGANCNNT TCNNGAAGTA GTGAGGAGGC    61 TTTTTTGGAG GCCTAGGCTT TTGCAAAAAG CTCCCGGGAG CTTGTATATC CATTTTCGGA   121 TCTGATCAGC ACGTGTTGAC AATTAATCAT CGGCATAGTA TATCGGCATA GTATAATACG   181 ACAAGGTGAG GAACTAAACC ATGGCCAAGT TGACCAGTGC CGTTCCGGTG CTCACCGCGC   241 GCGACGTCGC CGGAGCGGTC GAGTTCTGGA CCGACCGGCT CGGGTTCTCC CGGGACTTCG   301 TGGAGGACGA CTTCGCCGGT GTGGTCCGGG ACGACGTGAC CCTGTTCATC AGCGCGGTCC   361 AGGACCAGGT GGTGCCGGAC AACACCCTGG CCTGGGTGTG GGTGCGCGGC CTGGACGAGC   421 TGTACGCCGA GTGGTCGGAG GTCGTGTCCA CGAACTTCCG GGACGCCTCC GGGCCGGCCA   481 TGACCGAGAT CGGCGAGCAG CCGTGGGGGC GGGAGTTCGC CCTGCGCGAC CCGGCCGGCA   541 ACTGCGTGCA CTTCGTGGCC GAGGAGCAGG ACTGACACGT GCTACGAGAT TTCGATTCCA   601 CCGCCGCCTT CTATGAAAGG TTGGGCTTCG GAATCGTTTT CCGGGACGCC GGCTGGATGA   661 TCCTCCAGCG CGGGGATCTC ATGCTGGAGT TCTTCGCCCA CCCCAACTTG TTTATTGCAG   721 CTTATAATGG TTACNAATAA AGCAATAGCA TCACAAATTT CACAAATAAA GCATTTTTTT   781 CACTGCATTC TAGTTGTGGT TTGTCNNAAC TCATCAATGT ATCTTATCAT GTCTGTATAC   841 CGTCGACNTC TAGCTAGAGC TNGNCGTAAT CATGGNCATA GCTGTTTCCT GTGTGAAATT   901 GTTATCCGCT CACNNTTNCN CACAACATAC NANNCGGAAG CATAAAGTGN AAAGNNNGGG   961 GGNGCCNNAN GANNGANCNN ACNCACATNN ANTGCNTTGC GCNNNNNNNC NNNNTTNCNN 1021 NNNNNNAANN NNNNNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.05.22

GNP_filter3_RDBDEP_html_230512.pl