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CSII-EF-Venus

Lentivirus vector plasmid of Venus fluorescent protein driven by EF-1 alpha promoter.

Catalog number RDB05552
Resource name CSII-EF-Venus
Clone info. Lentivirus vector plasmid of Venus fluorescent protein driven by EF-1 alpha promoter.
Comment Commonly requested with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394).
Vector backbone CSII-EF-MCS (Plasmid)
Selectable markers Ampicillin (E. coli). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Gene/insert name Aequorea victoria GFP cDNA
Depositor|Developer Miyoshi, Hiroyuki |
 
Sequence (full) RDB05552hts01.seqcheck assembled from experimentally sequenced data
Remarks, protocol and/or map (pdf) RDB05552.pdf
Test sheet RDB18578_B0Kqp1-2.pdf 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Ordering instruction.[link] 
Terms and conditions for distribution The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer.
Remarks ((Remarks)) Remember that you will be working with samples containing infectious virus.
Please contact us. We will provide you with appropriate documents.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。
手続きの概要は、「レンチウイルスベクターの提供申し込み」をご覧ください。
MTAに書く使用条件 本件研究材料は、非営利機関の非営利学術研究に限って提供する。本件研究材料を利用した研究結果等を発表する際は、本件研究材料が故三好浩之博士により開発されたことを明示する。
備考 【使用上の注意】このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB05552 CSII-EF-Venus DNA solution

Ordering instruction of plasmids [in Japanese] [in English]

How to cite this biological resource

Materials & Methods section:

The CSII-EF-Venus was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB05552).

Reference section:

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

user_report Fukuda, T., Augmented Growth Hormone Secretion and Stat3 Phosphorylation in an Aryl Hydrocarbon Receptor Interacting Protein (AIP)-Disrupted Somatotroph Cell Line. PLoS One 11 (10): e0164131 (2016). PMID 27706259. [link to RRC of NBRP]
user_report Kimura, Y., CRISPR/Cas9-mediated reporter knock-in in mouse haploid embryonic stem cells. Sci. Rep. 5: 10710 (2015) PMID 26039937. [link to RRC of NBRP]
user_report Hasegawa, K., Retinoic acid signaling in Sertoli cells regulates organization of the blood-testis barrier through cyclical changes in gene expression. Development, 139 (23): 4347-4355 (2012). PMID 23095883. [link to RRC of NBRP]