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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

CSII-EF-Venus

Lentivirus vector plasmid of Venus fluorescent protein driven by EF-1 alpha promoter.

Catalog number RDB05552
Resource name CSII-EF-Venus
Clone info. Lentivirus vector plasmid of Venus fluorescent protein driven by EF-1 alpha promoter.
Comment Commonly requested with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Vector backbone CSII-EF-MCS (Plasmid)
Selectable markers Please note that Zeo resistance marker is not included in the lentivirus produced from this vector. E. coli: Amp^r.
Gene/insert name Aequorea victoria GFP cDNA
Depositor|Developer Miyoshi, Hiroyuki |
Other clones in our bank

External Database
Aequorea victoria GFP

            Reference sequence
              
            Lentiviral plasmid list. [link]

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer.
            Additional terms and conditions for distribution RECIPIENT is requested to obtain written consent of Dr. Atsushi Miyawaki of the RIKEN CBS regarding Venus fluorescent protein.
            Ordering Please visit Ordering instruction.[link] 
            Order form 
            [Credit Card Payment]  [Bank Transfer Payment]
            Material Transfer Agreement (MTA, for use for not-for-profit academic purpose)[Word]
            Approval form [Word] by Dr. Atsushi Miyawaki
            Remarks ((Remarks)) Remember that you will be working with samples containing infectious virus.
            Please contact us. We will provide you with appropriate documents.
            Please obtain written approval of Dr. Atsushi Miyawaki.
            提供案内 (日本国内) [open/close]

            提供条件 本件研究材料は、非営利機関の非営利学術研究に限って提供する。本件研究材料を利用した研究結果等を発表する際は、本件研究材料が故三好浩之博士により開発されたことを明示する。
            付加的提供条件 利用者は、Venus蛍光タンパク質について理化学研究所CBS 宮脇敦史博士から、書面による提供承諾を得ること。
            提供依頼 手続きの詳細は、「レンチウイルスベクターの提供申し込み」をご覧ください。
            提供依頼書 [Word]
            提供同意書 (非営利機関による非営利学術研究用)[Word]
            遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。
            宮脇敦史博士による提供承諾書 [Word]
            備考 【使用上の注意】このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。
            宮脇敦史博士の書面による提供承諾が必要です。

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB05552 CSII-EF-Venus DNA solution

            Ordering instruction of plasmids [in Japanese] [in English]
            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Electronic file

            Sequence RDB05552z.seq
            Remarks, protocol and/or map (pdf) RDB05552.pdf

            Original reference

            Further references such as user reports and related articles (go to bottom)

            Featured content


            Sequence information

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB18578_B0I9p1-1.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: EF1a-C_F
            Region: EF-1 alpha pro,Venus 5'
            Sequence file: RDB18578_B0I9a.seq check
            >05552_18578_B0I9_2_EF1a-C_F_A04_01_ABI24.ab1
                1 NNNNNNNNNN NNNNNNNTTT NANTTNGGNN CTTGGTTNNT TCTCNAGCCT CAGACAGTGG
               61 TTCAAAGTTT TTTTCTTCCA TTTCAGGTGT CGTGAACACG CTACCGGTCT CGAGAATTCT
              121 GCAGCGGCCG CGGATCCACC ATGGTGAGCA AGGGCGAGGA GCTGTTCACC GGGGTGGTGC
              181 CCATCCTGGT CGAGCTGGAC GGCGACGTAA ACGGCCACAA GTTCAGCGTG TCCGGCGAGG
              241 GCGAGGGCGA TGCCACCTAC GGCAAGCTGA CCCTGAAGCT GATCTGCACC ACCGGCAAGC
              301 TGCCCGTGCC CTGGCCCACC CTCGTGACCA CCCTGGGCTA CGGCCTGCAG TGCTTCGCCC
              361 GCTACCCCGA CCACATGAAG CAGCACGACT TCTTCAAGTC CGCCATGCCC GAAGGCTACG
              421 TCCAGGAGCG CACCATCTTC TTCAAGGACG ACGGCAACTA CAAGACCCGC GCCGAGGTGA
              481 AGTTCGAGGG CGACACCCTG GTGAACCGCA TCGAGCTGAA GGGCATCGAC TTCAAGGAGG
              541 ACGGCAACAT CCTGGGGCAC AAGCTGGAGT ACAACTACAA CAGCCACAAC GTCTATATCA
              601 CCGCCGACAA GCAGAAGAAC GGCATCAAGG CCAACTTCAA GATCCGCCAC AACATCGAGG
              661 ACGGCGGCGT GCAGCTCGCC GACCACTACC AGCAGAACAC CCCCATCGGC GACGGCCCCG
              721 TGCTGCTGCC CGACAACCAC TACCTGAGCT ACCAGTCCGC CCTGAGCAAA GACCCCAACG
              781 AGAAGCGCGA TCACATGGTC CTGCTGGAGT TCGTGACCGC CGCCGGGATC ACTCTCGGCA
              841 TGGACGANCT GTACAAGTAA GAATTCAAGG CCTCTCNAGC CTCTAGAGTT AACATCGAGG
              901 GATCNAGCTT ATCGATAATC AACCNCTGGA TTACAAANTT TGTGAAAGAN TGACTGGNAT
              961 TNNTAACTNN GTTGNNNCNT TTANNCTATG NGGANANNCN GNTTTAANGN CNNNNNNATC
            1021 ANGNNNNNGN NTNNCNNNNN NNNNN
            //
            Primer: PRE_rev
            Region: PRE,Venus 3'
            Sequence file: RDB18578_B0I9b.seq check
            >05552_18578_B0I9_2_PRE_rev_B04_04_ABI24.ab1
                1 NNNNNNNNNN NNNNNNGNNN NNNNANCNAN NTAGTTAAGA ATACCNGTCA ATCTTTCNCA
               61 AATTTTGTAA TCCAGAGGTT GATTATCGAT AAGCTTGATC CCTCGATGTT AACTCTAGAG
              121 GCTCGAGAGG CCTTGAATTC TTACTTGTAC AGCTCGTCCA TGCCGAGAGT GATCCCGGCG
              181 GCGGTCACGA ACTCCAGCAG GACCATGTGA TCGCGCTTCT CGTTGGGGTC TTTGCTCAGG
              241 GCGGACTGGT AGCTCAGGTA GTGGTTGTCG GGCAGCAGCA CGGGGCCGTC GCCGATGGGG
              301 GTGTTCTGCT GGTAGTGGTC GGCGAGCTGC ACGCCGCCGT CCTCGATGTT GTGGCGGATC
              361 TTGAAGTTGG CCTTGATGCC GTTCTTCTGC TTGTCGGCGG TGATATAGAC GTTGTGGCTG
              421 TTGTAGTTGT ACTCCAGCTT GTGCCCCAGG ATGTTGCCGT CCTCCTTGAA GTCGATGCCC
              481 TTCAGCTCGA TGCGGTTCAC CAGGGTGTCG CCCTCGAACT TCACCTCGGC GCGGGTCTTG
              541 TAGTTGCCGT CGTCCTTGAA GAAGATGGTG CGCTCCTGGA CGTAGCCTTC GGGCATGGCG
              601 GACTTGAAGA AGTCGTGCTG CTTCATGTGG TCGGGGTAGC GGGCGAAGCA CTGCAGGCCG
              661 TAGCCCAGGG TGGTCACGAG GGTGGGCCAG GGCACGGGCA GCTTGCCGGT GGTGCAGATC
              721 AGCTTCAGGG TCAGCTTGCC GTANGTGGCA TCGCCCTCGC CCTCGCCGGA CACGCTGAAC
              781 TTGTGGCCGT TTACGTCGCC GTCCAGCTCG ACNAGGATGG GCACCACCCC GGNGAACAGC
              841 TCCTCGCCCT TGCTCACCAT GGTGGATNNN CGGNCGCTGC AGAATTCTCG AGNNCGGTAN
              901 CGNGTNNACG ACACCNNAAA TGGAAGAAAA AAAACTTTGA NNNNCNNNCT GANGNTTGAG
              961 ANTGNACNNA NNNNNNNAAC TNAAAAAGGG NNAATTNNNN NNANNNNTAC NTNNNNNNNN
            1021 NN
            //
            Primer: CMV_Forward
            Region: CMV pro,5' LTR,HIV-1 psi
            Sequence file: RDB18578_B0I9c.seq check
            >05552_18578_B0I9_2_CMV_Forward_C04_07_ABI24.ab1
                1 NNNNNNNNNN NNNNNNNNNT GNNNCANTGG GCGGNAGGCG TGTACGGTGG GAGGTCTATA
               61 TAAGCAGCGC GTTTTGCCTG TACTGGGTCT CTCTGGTTAG ACCAGATCTG AGCCTGGGAG
              121 CTCTCTGGCT AACTAGGGAA CCCACTGCTT AAGCCTCAAT AAAGCTTGCC TTGAGTGCTT
              181 CAAGTAGTGT GTGCCCGTCT GTTGTGTGAC TCTGGTAACT AGAGATCCCT CAGACCCTTT
              241 TAGTCAGTGT GGAAAATCTC TAGCAGTGGC GCCCGAACAG GGACTTGAAA GCGAAAGGGA
              301 AACCAGAGGA GCTCTCTCGA CGCAGGACTC GGCTTGCTGA AGCGCGCACG GCAAGAGGCG
              361 AGGGGCGGCG ACTGGTGAGT ACGCCAAAAA TTTTGACTAG CGGAGGCTAG AAGGANNNAG
              421 ATGGGTGCGA GAGCGTCAGT ATTAAGCGGG GGANAATTAG NTCGCGATGG GAAAAANTTC
              481 GGNTNANGNC NGGGGGAAAN AAAAAATNTN ANTTNAANCN TNTNNTANGG GNANNCNNGG
            541 ANCTNNANNN ATTCNNANTN NNNNCNNGNN NNNNNNNN
            //
            Primer: cPPT/CTS-E_R
            Region: cPPT_CTS,RRE
            Sequence file: RDB18578_B0I9d.seq check
            >05552_18578_B0I9_2_cPPT+CTS-E_R_D04_10_ABI24.ab1
                1 NNNNNNNNNN NNNNNNNNNN NNNNNTTCTT NAGTTTGTAT GTCTGTTGCT ATTATGTCTA
               61 CTATTCTTTC CCCTGCACTG TACCCCCCAA TCCCCCCTTT TCTTTTAAAA TTGTGGATGA
              121 ATACTGCCAT TTGTAGATCC GTTCACTAAT CGAATGGATC TGTCTCTGTC TCTCTCTCCA
              181 CCTTCTTCTT CTATTCCTTC GGGCCTGTCG GGTCCCCTCG GGGTTGGGAG GTGGGTCTGA
              241 AACGATAATG GTGAATATCC CTGCCTAACT CTATTCACTA TAGAAAGTAC AGCAAAAACT
              301 ATTCTTAAAC CTACCAAGCC TCCTACTATC ATTATGAATA ATTTTATATA CCACAGCCAA
              361 TTTGTTATGT TAAACCAATT CCACAAACTT GCCCATTTAT CTAATTCCAA TAATTCTTGT
              421 TCATTCTTTT CTTGCTGGTT TTGCGATTCT TCAATTAAGG AGTGTATTAA GCTTGTGTAA
              481 TTGTTAATTT CTCTGTCCCA CTCCATCCAG GTCGTGTGAT TCCAAATCTG TTCCAGAGAT
              541 TTATTACTCC AACTAGCATT CCAAGGCACA GCAGTGGTGC AAATGAGTTT TCCAGAGCAA
              601 CCCCAAATCC CCAGGAGCTG TTGATCCTTT AGGTATCTTT CCACAGCCAG GATTCTTGCC
              661 TGGAGCTGCT TGATGCCCCA GACTGTGAGT TGCAACAGAT GCTGTTGCGC CTCAATAGCC
              721 CTCAGCAAAT TGTTCTGCTG CTGCACTATA CCAGACAATA ATTGTCTGGC CTGTACCGTC
              781 AGCGTCATTG ACGCTGCGCC CATAGTGCTT CCTGCTGCTC CCAAGAANCC ANNGAACAAA
              841 GCTCCTATTC CCACNGCTCT TTTTTCTCTN TGCNNCNACT CTTCTCTTTN CCNTGGTGGG
              901 NGCTACTCCT AANGGTTCAN NTTTTNANNA CTTTATATTT ATATAANTCA CTTCTNCNAN
              961 NNGTCCCTCA NATCTNCNTC NNCCANGNCT GNNNATNANN NGNCCCTTGC TGNGNNGNGN
            1021 NNNTNNNTTT TNNTNNNNNN TNNNNNNNNN N
            //
            Primer: bGH_rev3
            Region: bGHpA,3' LTR,PRE
            Sequence file: RDB18578_B0I9e.seq check
            >05552_18578_B0I9_2_bGH_rev3_E04_13_ABI24.ab1
                1 NNNNNNNNNN NNNNANNNNN NNNGGGNNGG GNNNANNNCA GATGGCTGGC AACTAGAAGG
               61 CACAGTCGAG GCTGATCAGC GGGTTTAAAC GGGCCCTGCT AGAGATTTTC CACACTGACT
              121 AAAAGGGTCT GAGGGATCTC TAGTTACCAG AGTCACACAA CAGACGGGCA CACACTACTT
              181 GAAGCACTCA AGGCAAGCTT TATTGAGGCT TAAGCAGTGG GTTCCCTAGT TAGCCAGAGA
              241 GCTCCCAGGC TCAGATCTGG TCTAACCAGA GAGACCCAGT ACAGTCCGGA TGCAGCTCTC
              301 GGGCCATGTG ATGAAATGCT AGGCGGCTGT CAAACCTCCA CTCTAATACT TCTCTCTCCG
              361 GGTCATCCAT CCCATGCAGG CTCACAGGGT GTAACAAGCG GGTGTTCTCT CCTTCATTGG
              421 CTTCTTCTAC CTTCTCTTGC TCAACTGGTA CTAGCTTGTA GCACCATCCA AAGGTCAGTG
              481 GATATCTGAT CCCTGGCCCT GGTGTGTAGT TCTGCCAATC AGGGAAGTAG CCTTGTGTGT
              541 GGTAGATCCA CAGATCAAGG ATATCTTGTC TTCGTTGGGA GTGAATTAGC CCTTCCAGTC
              601 CCCCCTTTTC TTTTAAAAAG TGGCTAAGAT CTACAGCTGC CTTGTAAGTC ATTGGTCTTA
              661 AAGGTACCTG AGGTGTGACT GGAAAACCCA CCTCCTCCTC CTCTTGTGCT TCTAGCCAGG
              721 CACAATCAGC ATTGGTAGCT GCTGTATTGC TACTTGTGAT TGCTCCATGT TTTTCTAGGT
              781 CTCGACGGTA TCGATGCGGG GANGCGGCCC AAAGGGANNT CCGACTCGTC TGANGGCGAA
              841 NNCGAANANG CGGAAGANGC CNCANANNCN GCANCANGNN NCGGGAAGGA ANGNNCGCTN
            901 GATTGAGGGN CGAANGGNNN TNNNNNNNNN N
            //
            Primer: SV40pro_ori_F
            Region: SV40 pro_ori,Zeo
            Sequence file: RDB18578_B0I9f.seq check
            >05552_18578_B0I9_2_SV40pro_ori_F_F04_16_ABI24.ab1
                1 NNNNNNNNNN NNNNNNNNNC NNCCTCTGCC TCTGANCNNT TCNNGAAGTA GTGAGGAGGC
               61 TTTTTTGGAG GCCTAGGCTT TTGCAAAAAG CTCCCGGGAG CTTGTATATC CATTTTCGGA
              121 TCTGATCAGC ACGTGTTGAC AATTAATCAT CGGCATAGTA TATCGGCATA GTATAATACG
              181 ACAAGGTGAG GAACTAAACC ATGGCCAAGT TGACCAGTGC CGTTCCGGTG CTCACCGCGC
              241 GCGACGTCGC CGGAGCGGTC GAGTTCTGGA CCGACCGGCT CGGGTTCTCC CGGGACTTCG
              301 TGGAGGACGA CTTCGCCGGT GTGGTCCGGG ACGACGTGAC CCTGTTCATC AGCGCGGTCC
              361 AGGACCAGGT GGTGCCGGAC AACACCCTGG CCTGGGTGTG GGTGCGCGGC CTGGACGAGC
              421 TGTACGCCGA GTGGTCGGAG GTCGTGTCCA CGAACTTCCG GGACGCCTCC GGGCCGGCCA
              481 TGACCGAGAT CGGCGAGCAG CCGTGGGGGC GGGAGTTCGC CCTGCGCGAC CCGGCCGGCA
              541 ACTGCGTGCA CTTCGTGGCC GAGGAGCAGG ACTGACACGT GCTACGAGAT TTCGATTCCA
              601 CCGCCGCCTT CTATGAAAGG TTGGGCTTCG GAATCGTTTT CCGGGACGCC GGCTGGATGA
              661 TCCTCCAGCG CGGGGATCTC ATGCTGGAGT TCTTCGCCCA CCCCAACTTG TTTATTGCAG
              721 CTTATAATGG TTACNAATAA AGCAATAGCA TCACAAATTT CACAAATAAA GCATTTTTTT
              781 CACTGCATTC TAGTTGTGGT TTGTCNNAAC TCATCAATGT ATCTTATCAT GTCTGTATAC
              841 CGTCGACNTC TAGCTAGAGC TNGNCGTAAT CATGGNCATA GCTGTTTCCT GTGTGAAATT
              901 GTTATCCGCT CACNNTTNCN CACAACATAC NANNCGGAAG CATAAAGTGN AAAGNNNGGG
              961 GGNGCCNNAN GANNGANCNN ACNCACATNN ANTGCNTTGC GCNNNNNNNC NNNNTTNCNN
            1021 NNNNNNAANN NNNNNNNN
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            user_report Fukuda, T., Augmented Growth Hormone Secretion and Stat3 Phosphorylation in an Aryl Hydrocarbon Receptor Interacting Protein (AIP)-Disrupted Somatotroph Cell Line. PLoS One 11 (10): e0164131 (2016). PMID 27706259.
            user_report Kimura, Y., CRISPR/Cas9-mediated reporter knock-in in mouse haploid embryonic stem cells. Sci. Rep. 5: 10710 (2015) PMID 26039937.
            user_report Hasegawa, K., Retinoic acid signaling in Sertoli cells regulates organization of the blood-testis barrier through cyclical changes in gene expression. Development, 139 (23): 4347-4355 (2012). PMID 23095883.

            2022.05.18

            GNP_filter3_RDBDEP_html_220516.pl