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CSII-EF-Venus

Lentivirus vector plasmid of Venus fluorescent protein driven by EF-1 alpha promoter.

Catalog number RDB05552
Resource name CSII-EF-Venus
Clone info. Lentivirus vector plasmid of Venus fluorescent protein driven by EF-1 alpha promoter.
Commonly requested with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Vector backbone CSII-EF-MCS (Plasmid)
Selectable markers Please note that Zeo resistance marker is not included in the lentivirus produced from this vector. E. coli: Amp^r.
Gene/insert name Aequorea victoria GFP cDNA
Depositor|Developer Miyoshi, Hiroyuki |
Lentiviral plasmid list, maps and sequences information. [link]

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer.
Additional terms and conditions for distribution RECIPIENT is requested to obtain written consent of Dr. Atsushi Miyawaki of the RIKEN CBS regarding Venus fluorescent protein.
Ordering Please visit Ordering instruction.[link] 
Order form 
[Credit Card Payment]  [Bank Transfer or Check Payment]
Material Transfer Agreement (MTA, for use for not-for-profit academic purpose)[Word]
Approval form [Word] by Dr. Atsushi Miyawaki
Remarks ((Remarks)) Remember that you will be working with samples containing infectious virus.
Please contact us. We will provide you with appropriate documents.
Please obtain written approval of Dr. Atsushi Miyawaki.
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB05552 CSII-EF-Venus DNA solution

Ordering instruction of plasmids [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Electronic file Sequence RDB05552z.seq
Electronic file Remarks, protocol and/or map (pdf) RDB05552.pdf

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content


Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB18578_B0I9p1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: EF1a-C_F
Region: EF-1 alpha pro,Venus 5'
Sequence file: RDB18578_B0I9a.seq check
>05552_18578_B0I9_2_EF1a-C_F_A04_01_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNTTT NANTTNGGNN CTTGGTTNNT TCTCNAGCCT CAGACAGTGG
   61 TTCAAAGTTT TTTTCTTCCA TTTCAGGTGT CGTGAACACG CTACCGGTCT CGAGAATTCT
  121 GCAGCGGCCG CGGATCCACC ATGGTGAGCA AGGGCGAGGA GCTGTTCACC GGGGTGGTGC
  181 CCATCCTGGT CGAGCTGGAC GGCGACGTAA ACGGCCACAA GTTCAGCGTG TCCGGCGAGG
  241 GCGAGGGCGA TGCCACCTAC GGCAAGCTGA CCCTGAAGCT GATCTGCACC ACCGGCAAGC
  301 TGCCCGTGCC CTGGCCCACC CTCGTGACCA CCCTGGGCTA CGGCCTGCAG TGCTTCGCCC
  361 GCTACCCCGA CCACATGAAG CAGCACGACT TCTTCAAGTC CGCCATGCCC GAAGGCTACG
  421 TCCAGGAGCG CACCATCTTC TTCAAGGACG ACGGCAACTA CAAGACCCGC GCCGAGGTGA
  481 AGTTCGAGGG CGACACCCTG GTGAACCGCA TCGAGCTGAA GGGCATCGAC TTCAAGGAGG
  541 ACGGCAACAT CCTGGGGCAC AAGCTGGAGT ACAACTACAA CAGCCACAAC GTCTATATCA
  601 CCGCCGACAA GCAGAAGAAC GGCATCAAGG CCAACTTCAA GATCCGCCAC AACATCGAGG
  661 ACGGCGGCGT GCAGCTCGCC GACCACTACC AGCAGAACAC CCCCATCGGC GACGGCCCCG
  721 TGCTGCTGCC CGACAACCAC TACCTGAGCT ACCAGTCCGC CCTGAGCAAA GACCCCAACG
  781 AGAAGCGCGA TCACATGGTC CTGCTGGAGT TCGTGACCGC CGCCGGGATC ACTCTCGGCA
  841 TGGACGANCT GTACAAGTAA GAATTCAAGG CCTCTCNAGC CTCTAGAGTT AACATCGAGG
  901 GATCNAGCTT ATCGATAATC AACCNCTGGA TTACAAANTT TGTGAAAGAN TGACTGGNAT
  961 TNNTAACTNN GTTGNNNCNT TTANNCTATG NGGANANNCN GNTTTAANGN CNNNNNNATC
1021 ANGNNNNNGN NTNNCNNNNN NNNNN
//
Primer: PRE_rev
Region: PRE,Venus 3'
Sequence file: RDB18578_B0I9b.seq check
>05552_18578_B0I9_2_PRE_rev_B04_04_ABI24.ab1
    1 NNNNNNNNNN NNNNNNGNNN NNNNANCNAN NTAGTTAAGA ATACCNGTCA ATCTTTCNCA
   61 AATTTTGTAA TCCAGAGGTT GATTATCGAT AAGCTTGATC CCTCGATGTT AACTCTAGAG
  121 GCTCGAGAGG CCTTGAATTC TTACTTGTAC AGCTCGTCCA TGCCGAGAGT GATCCCGGCG
  181 GCGGTCACGA ACTCCAGCAG GACCATGTGA TCGCGCTTCT CGTTGGGGTC TTTGCTCAGG
  241 GCGGACTGGT AGCTCAGGTA GTGGTTGTCG GGCAGCAGCA CGGGGCCGTC GCCGATGGGG
  301 GTGTTCTGCT GGTAGTGGTC GGCGAGCTGC ACGCCGCCGT CCTCGATGTT GTGGCGGATC
  361 TTGAAGTTGG CCTTGATGCC GTTCTTCTGC TTGTCGGCGG TGATATAGAC GTTGTGGCTG
  421 TTGTAGTTGT ACTCCAGCTT GTGCCCCAGG ATGTTGCCGT CCTCCTTGAA GTCGATGCCC
  481 TTCAGCTCGA TGCGGTTCAC CAGGGTGTCG CCCTCGAACT TCACCTCGGC GCGGGTCTTG
  541 TAGTTGCCGT CGTCCTTGAA GAAGATGGTG CGCTCCTGGA CGTAGCCTTC GGGCATGGCG
  601 GACTTGAAGA AGTCGTGCTG CTTCATGTGG TCGGGGTAGC GGGCGAAGCA CTGCAGGCCG
  661 TAGCCCAGGG TGGTCACGAG GGTGGGCCAG GGCACGGGCA GCTTGCCGGT GGTGCAGATC
  721 AGCTTCAGGG TCAGCTTGCC GTANGTGGCA TCGCCCTCGC CCTCGCCGGA CACGCTGAAC
  781 TTGTGGCCGT TTACGTCGCC GTCCAGCTCG ACNAGGATGG GCACCACCCC GGNGAACAGC
  841 TCCTCGCCCT TGCTCACCAT GGTGGATNNN CGGNCGCTGC AGAATTCTCG AGNNCGGTAN
  901 CGNGTNNACG ACACCNNAAA TGGAAGAAAA AAAACTTTGA NNNNCNNNCT GANGNTTGAG
  961 ANTGNACNNA NNNNNNNAAC TNAAAAAGGG NNAATTNNNN NNANNNNTAC NTNNNNNNNN
1021 NN
//
Primer: CMV_Forward
Region: CMV pro,5' LTR,HIV-1 psi
Sequence file: RDB18578_B0I9c.seq check
>05552_18578_B0I9_2_CMV_Forward_C04_07_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNT GNNNCANTGG GCGGNAGGCG TGTACGGTGG GAGGTCTATA
   61 TAAGCAGCGC GTTTTGCCTG TACTGGGTCT CTCTGGTTAG ACCAGATCTG AGCCTGGGAG
  121 CTCTCTGGCT AACTAGGGAA CCCACTGCTT AAGCCTCAAT AAAGCTTGCC TTGAGTGCTT
  181 CAAGTAGTGT GTGCCCGTCT GTTGTGTGAC TCTGGTAACT AGAGATCCCT CAGACCCTTT
  241 TAGTCAGTGT GGAAAATCTC TAGCAGTGGC GCCCGAACAG GGACTTGAAA GCGAAAGGGA
  301 AACCAGAGGA GCTCTCTCGA CGCAGGACTC GGCTTGCTGA AGCGCGCACG GCAAGAGGCG
  361 AGGGGCGGCG ACTGGTGAGT ACGCCAAAAA TTTTGACTAG CGGAGGCTAG AAGGANNNAG
  421 ATGGGTGCGA GAGCGTCAGT ATTAAGCGGG GGANAATTAG NTCGCGATGG GAAAAANTTC
  481 GGNTNANGNC NGGGGGAAAN AAAAAATNTN ANTTNAANCN TNTNNTANGG GNANNCNNGG
541 ANCTNNANNN ATTCNNANTN NNNNCNNGNN NNNNNNNN
//
Primer: cPPT/CTS-E_R
Region: cPPT_CTS,RRE
Sequence file: RDB18578_B0I9d.seq check
>05552_18578_B0I9_2_cPPT+CTS-E_R_D04_10_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNTTCTT NAGTTTGTAT GTCTGTTGCT ATTATGTCTA
   61 CTATTCTTTC CCCTGCACTG TACCCCCCAA TCCCCCCTTT TCTTTTAAAA TTGTGGATGA
  121 ATACTGCCAT TTGTAGATCC GTTCACTAAT CGAATGGATC TGTCTCTGTC TCTCTCTCCA
  181 CCTTCTTCTT CTATTCCTTC GGGCCTGTCG GGTCCCCTCG GGGTTGGGAG GTGGGTCTGA
  241 AACGATAATG GTGAATATCC CTGCCTAACT CTATTCACTA TAGAAAGTAC AGCAAAAACT
  301 ATTCTTAAAC CTACCAAGCC TCCTACTATC ATTATGAATA ATTTTATATA CCACAGCCAA
  361 TTTGTTATGT TAAACCAATT CCACAAACTT GCCCATTTAT CTAATTCCAA TAATTCTTGT
  421 TCATTCTTTT CTTGCTGGTT TTGCGATTCT TCAATTAAGG AGTGTATTAA GCTTGTGTAA
  481 TTGTTAATTT CTCTGTCCCA CTCCATCCAG GTCGTGTGAT TCCAAATCTG TTCCAGAGAT
  541 TTATTACTCC AACTAGCATT CCAAGGCACA GCAGTGGTGC AAATGAGTTT TCCAGAGCAA
  601 CCCCAAATCC CCAGGAGCTG TTGATCCTTT AGGTATCTTT CCACAGCCAG GATTCTTGCC
  661 TGGAGCTGCT TGATGCCCCA GACTGTGAGT TGCAACAGAT GCTGTTGCGC CTCAATAGCC
  721 CTCAGCAAAT TGTTCTGCTG CTGCACTATA CCAGACAATA ATTGTCTGGC CTGTACCGTC
  781 AGCGTCATTG ACGCTGCGCC CATAGTGCTT CCTGCTGCTC CCAAGAANCC ANNGAACAAA
  841 GCTCCTATTC CCACNGCTCT TTTTTCTCTN TGCNNCNACT CTTCTCTTTN CCNTGGTGGG
  901 NGCTACTCCT AANGGTTCAN NTTTTNANNA CTTTATATTT ATATAANTCA CTTCTNCNAN
  961 NNGTCCCTCA NATCTNCNTC NNCCANGNCT GNNNATNANN NGNCCCTTGC TGNGNNGNGN
1021 NNNTNNNTTT TNNTNNNNNN TNNNNNNNNN N
//
Primer: bGH_rev3
Region: bGHpA,3' LTR,PRE
Sequence file: RDB18578_B0I9e.seq check
>05552_18578_B0I9_2_bGH_rev3_E04_13_ABI24.ab1
    1 NNNNNNNNNN NNNNANNNNN NNNGGGNNGG GNNNANNNCA GATGGCTGGC AACTAGAAGG
   61 CACAGTCGAG GCTGATCAGC GGGTTTAAAC GGGCCCTGCT AGAGATTTTC CACACTGACT
  121 AAAAGGGTCT GAGGGATCTC TAGTTACCAG AGTCACACAA CAGACGGGCA CACACTACTT
  181 GAAGCACTCA AGGCAAGCTT TATTGAGGCT TAAGCAGTGG GTTCCCTAGT TAGCCAGAGA
  241 GCTCCCAGGC TCAGATCTGG TCTAACCAGA GAGACCCAGT ACAGTCCGGA TGCAGCTCTC
  301 GGGCCATGTG ATGAAATGCT AGGCGGCTGT CAAACCTCCA CTCTAATACT TCTCTCTCCG
  361 GGTCATCCAT CCCATGCAGG CTCACAGGGT GTAACAAGCG GGTGTTCTCT CCTTCATTGG
  421 CTTCTTCTAC CTTCTCTTGC TCAACTGGTA CTAGCTTGTA GCACCATCCA AAGGTCAGTG
  481 GATATCTGAT CCCTGGCCCT GGTGTGTAGT TCTGCCAATC AGGGAAGTAG CCTTGTGTGT
  541 GGTAGATCCA CAGATCAAGG ATATCTTGTC TTCGTTGGGA GTGAATTAGC CCTTCCAGTC
  601 CCCCCTTTTC TTTTAAAAAG TGGCTAAGAT CTACAGCTGC CTTGTAAGTC ATTGGTCTTA
  661 AAGGTACCTG AGGTGTGACT GGAAAACCCA CCTCCTCCTC CTCTTGTGCT TCTAGCCAGG
  721 CACAATCAGC ATTGGTAGCT GCTGTATTGC TACTTGTGAT TGCTCCATGT TTTTCTAGGT
  781 CTCGACGGTA TCGATGCGGG GANGCGGCCC AAAGGGANNT CCGACTCGTC TGANGGCGAA
  841 NNCGAANANG CGGAAGANGC CNCANANNCN GCANCANGNN NCGGGAAGGA ANGNNCGCTN
901 GATTGAGGGN CGAANGGNNN TNNNNNNNNN N
//
Primer: SV40pro_ori_F
Region: SV40 pro_ori,Zeo
Sequence file: RDB18578_B0I9f.seq check
>05552_18578_B0I9_2_SV40pro_ori_F_F04_16_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNC NNCCTCTGCC TCTGANCNNT TCNNGAAGTA GTGAGGAGGC
   61 TTTTTTGGAG GCCTAGGCTT TTGCAAAAAG CTCCCGGGAG CTTGTATATC CATTTTCGGA
  121 TCTGATCAGC ACGTGTTGAC AATTAATCAT CGGCATAGTA TATCGGCATA GTATAATACG
  181 ACAAGGTGAG GAACTAAACC ATGGCCAAGT TGACCAGTGC CGTTCCGGTG CTCACCGCGC
  241 GCGACGTCGC CGGAGCGGTC GAGTTCTGGA CCGACCGGCT CGGGTTCTCC CGGGACTTCG
  301 TGGAGGACGA CTTCGCCGGT GTGGTCCGGG ACGACGTGAC CCTGTTCATC AGCGCGGTCC
  361 AGGACCAGGT GGTGCCGGAC AACACCCTGG CCTGGGTGTG GGTGCGCGGC CTGGACGAGC
  421 TGTACGCCGA GTGGTCGGAG GTCGTGTCCA CGAACTTCCG GGACGCCTCC GGGCCGGCCA
  481 TGACCGAGAT CGGCGAGCAG CCGTGGGGGC GGGAGTTCGC CCTGCGCGAC CCGGCCGGCA
  541 ACTGCGTGCA CTTCGTGGCC GAGGAGCAGG ACTGACACGT GCTACGAGAT TTCGATTCCA
  601 CCGCCGCCTT CTATGAAAGG TTGGGCTTCG GAATCGTTTT CCGGGACGCC GGCTGGATGA
  661 TCCTCCAGCG CGGGGATCTC ATGCTGGAGT TCTTCGCCCA CCCCAACTTG TTTATTGCAG
  721 CTTATAATGG TTACNAATAA AGCAATAGCA TCACAAATTT CACAAATAAA GCATTTTTTT
  781 CACTGCATTC TAGTTGTGGT TTGTCNNAAC TCATCAATGT ATCTTATCAT GTCTGTATAC
  841 CGTCGACNTC TAGCTAGAGC TNGNCGTAAT CATGGNCATA GCTGTTTCCT GTGTGAAATT
  901 GTTATCCGCT CACNNTTNCN CACAACATAC NANNCGGAAG CATAAAGTGN AAAGNNNGGG
  961 GGNGCCNNAN GANNGANCNN ACNCACATNN ANTGCNTTGC GCNNNNNNNC NNNNTTNCNN
1021 NNNNNNAANN NNNNNNNN
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

user report Fukuda, T., Augmented Growth Hormone Secretion and Stat3 Phosphorylation in an Aryl Hydrocarbon Receptor Interacting Protein (AIP)-Disrupted Somatotroph Cell Line. PLoS One 11 (10): e0164131 (2016). PMID 27706259.
user report Kimura, Y., CRISPR/Cas9-mediated reporter knock-in in mouse haploid embryonic stem cells. Sci. Rep. 5: 10710 (2015) PMID 26039937.
user report Hasegawa, K., Retinoic acid signaling in Sertoli cells regulates organization of the blood-testis barrier through cyclical changes in gene expression. Development, 139 (23): 4347-4355 (2012). PMID 23095883.

2020.12.24

GNP_filter3_RDBDEP_html_201222.pl