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pKM2L-phUPA (#RDB05547)

Promoter Bank clone, Human urokinase plasminogen activtor (uPA) promoter

Alternative name p1966-9F
Clone info. PCR amplified human urokinase plasminogen activtor (uPA) promoter sequence was inserted into a Renilla luciferase reporter vector pKM2L (RDB04026). Genomic sequence from 57961 to 60063 of AL596247.22 corresponding -2068 to +35 (the transcription start site is designated +1. Fig. 1 of Verde, P. Nucleic Acids Res. 16 (22): 10699-10716, 1988) is cloned. Alternatively, 73909061 to 73911163(NC_000010.11); relatively -2071 to +32, where +1 corresponds to 1 nt of NM_002658.5 was cloned.
Comment PCR cloning, forward primer, 1966 F2: 5' gtgctaggtgaaaggtggcacgggg 3'; reverse primer, 1966 R2: 5' cccgggctccggggctgcggtctcc 3' Entire sequence of promoter region has not been confirmed.
Vector backbone pKM2L (RDB04026) (Plasmid)
Size of vector backbone 4.2 kb
Selectable markers Kan^r
Gene/insert name Human urokinase plasminogen activtor (uPA) genomic DNA
Depositor|Developer DNA Bank, |
 

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The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
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Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB05547 pKM2L-phUPA Under QC test. Please contact us. DNA solution

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How to cite this biological resource

Materials & Methods section:

The pKM2L-phUPA was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB05547).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

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References

Original, user report and related articles

reference Verde, P., An upstream enhancer and a negative element in the 5' flanking region of the human urokinase plasminogen activator gene. Nucleic Acids Res. 16 (22): 10699-10716 (1988). PMID 3205721.
user_report Shimizu, M., Plasminogen activator uPA is a direct transcriptional target of the JAG1-Notch receptor signaling pathway in breast cancer. Cancer Res., 71, 277-286 (2011) . PMID 21199807. [link to RRC of NBRP]