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pKM2L-phPSA (#RDB05546)

Promoter Bank clone, Human prostate-specific antigen/kallikrein 3 (PSA/KLK3) promoter

Alternative name p1965-7F
Clone info. PCR amplified human prostate-specific antigen/kallikrein 3 (PSA/KLK3) promoter sequence was inserted into a Renilla luciferase reporter vector pKM2L (RDB04026). Genomic sequence from 22630 to 23369 of AC011523.3 corresponding -707 to +33 (the transcription start site is designated +1. Fig.1 of Cleutjens, K.B. J. Biol. Chem. 271 (11): 6379-6388, 1996) is cloned. Alternatively, 50854208 to 50854947(NC_000019.10); relatively -707 to +33, where +1 corresponds to 1 nt of NM_001648.2 was cloned.
Comment PCR cloning, forward primer, 1965 F3: 5' agggcaggtcagcaggcatctct 3'; reverse primer, 1965 R3: 5' agctctccgggtgcaggtggtaa 3' Entire sequence of promoter region has not been confirmed.
Vector backbone pKM2L (RDB04026) (Plasmid)
Size of vector backbone 4.2 kb
Selectable markers Kan^r
Gene/insert name Human prostate-specific antigen/kallikrein 3 (PSA/KLK3) genomic DNA
Depositor|Developer DNA Bank, |
 
Sequence RDB05546z.seq
Remarks, protocol and/or map (pdf) RDB05546.pdf

Distribution information

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RDB05546 pKM2L-phPSA DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pKM2L-phPSA was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB05546).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB12756_A4Df.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: M13_-40
Sequence file: RDB12756_A4Dfa.seq check
>05546_12756_A4Df_M13(-40)_E01_13.ab1
    1 CGGGGCCGGG CCCAGGCTTG CATGCCTGCA GGTCATTTAA TAGGGATAAC AGGGTAATGA
   61 TTAAGATCTC GAGCTCTAAG CTTCACATAT GCATGCACTA GTGGCAGGGC AGGTCAGCAG
  121 GCATCTCTGT TGCACAGATA GAGTGCACAG GTCTGGAGAA CAAGGAGTGG GGGGTTATTG
  181 GAATTCCACA TTGTTTGCTG CACGTTGGAT TTTGAAATGC TAGGGAACTT TGGGAGACTC
  241 ATATTTCTGG GCTAGAGGAT CTGTGGACCA CAAGATCTTT TTATGATGAC AGTAGCAATG
  301 TATCTGTGGA GCTGGATTCT GGGTTGGGAG TGCAAGGAAA AGAATGTACT AAATGCCAAG
  361 ACATCTATTT CAGGAGCATG AGGAATAAAA GTTCTAGTTT CTGGTCTCAG AGTGGTGCAG
  421 GGATCAGGGA GTCTCACAAT CTCCTGAGTG CTGGTGTTTT AGGGCACACT GGGTCTTGGA
  481 GTGCAAAGGA TCTAGGCACG TGAGGCTTTG TATGAAGAAT CGGGGATCAT ACCCACCCCC
  541 TGTTTCTGTT TCATCCTGGG CGTGTCTCCT CTGCCTTTGT CCCCTAGATG AAGTCTCCAT
  601 GAGCTACAGG GCCTGGTGCA TCCAGGGTGA TCTAGTAATT GCAGAACAGC AAGTGCTAGC
  661 TCTCCCTCCC CTTCCACAGC TCTGGGTGTG GGAGGGGGTT GTCCAGCCTC CAGCAGCATG
  721 GGGAGGGCCT TGGTCAGCCT CTGGGTGCCA GCAGGGCAGG GGCGGAGTCC TGGGGAATGA
  781 AGGTTTTATA GGGCTCCTGG GGGAGGCTCC CCAGCCCCAA GCTTACCACC TGCACCCGGA
  841 GAGCTGCCTG TCGACGCGTA GAATTCACCC GGGTACTGCA GAAGTTGGTC GTGAGGCACT
  901 GGGCAGGTAA GTATCAAGGT TACAAGACAG GTTTAAGGAG ACCAATAGAA ACTGGGCTTG
  961 TCGAGACCAG AGAAGACTCT TTGCGTTTCT GATAGGCCAC CTATTGGTCT TACTGAACAT
 1021 CCACTTTTGC CTTTTCTTCT TCCACCAGGT GTCCCACTCC CCAGTTTCAA TTTACAGCTC
 1081 TTAAAGGGCT AAGAAGTTAC CTTTAAATAC GACTTCACTA TCAGGCTTAG CGCAAACAAT
1141 TGGCACTTCC CCAAAGGTGT ACGAAAC
//
Primer: phRLR2
Sequence file: RDB12756_A4Dfb.seq check
>05546_12756_A4Df_phRLR2_F01_16.ab1
    1 AACTTCCTGT CTCGACAGCC CAGTTTCTAT TGGTCTCCTT AAACCTGTCT TGTAACCTTG
   61 ATACTTACCT GCCCAGTGCC TCACGACCAA CTTCTGCAGT ACCCGGGTGA ATTCTACGCG
  121 TCGACAGGCA GCTCTCCGGG TGCAGGTGGT AAGCTTGGGG CTGGGGAGCC TCCCCCAGGA
  181 GCCCTATAAA ACCTTCATTC CCCAGGACTC CGCCCCTGCC CTGCTGGCAC CCAGAGGCTG
  241 ACCAAGGCCC TCCCCATGCT GCTGGAGGCT GGACAACCCC CTCCCACACC CAGAGCTGTG
  301 GAAGGGGAGG GAGAGCTAGC ACTTGCTGTT CTGCAATTAC TAGATCACCC TGGATGCACC
  361 AGGCCCTGTA GCTCATGGAG ACTTCATCTA GGGGACAAAG GCAGAGGAGA CACGCCCAGG
  421 ATGAAACAGA AACAGGGGGT GGGTATGATC CCCGATTCTT CATACAAAGC CTCACGTGCC
  481 TAGATCCTTT GCACTCCAAG ACCCAGTGTG CCCTAAAACA CCAGCACTCA GGAGATTGTG
  541 AGACTCCCTG ATCCCTGCAC CACTCTGAGA CCAGAAACTA GAACTTTTAT TCCTCATGCT
  601 CCTGAAATAG ATGTCTTGGC ATTTAGTACA TTCTTTTCCT TGCACTCCCA ACCCAGAATC
  661 CAGCTCCACA GATACATTGC TACTGTCATC ATAAAAAGAT CTTGTGGTCC ACAGATCCTC
  721 TAGCCCAGAA ATATGAGTCT CCCAAAGTTC CCTAGCATTT CAAAATCCAA CGTGCAGCAA
  781 ACAATGTGGA ATTCCAATAA CCCCCCACTC CTTGTTCTCC AGACCTGTGC ACTCTATCTG
  841 TGCAACAGAG ATGCCTGCTG ACCTGCCCTG CCACTAGTGC ATGCATATGT GAAGCTTAGA
  901 GCTCGAGATC TTAATCATTA CCCTGTTATC CCTATTTAAA TGACCTGCAG GCATGCAAGC
  961 TTGGCACTGG CCGTCGTTTT ACAACGTCGT GACTGGGAAA ACCCTGGCGT TACCCAACTT
 1021 AATCGCCTTT GCAGCACATC CCCCTTTCGC CAGCTGGCGT AATAGCGAAG AGGCCCGGCA
 1081 CGATCGCCGT TCCACAGTTG CGCAGCTGAT GGCGATGCCG ATTTATTCCA CCAAAGGCCG
1141 CCCGATCCCC GTCAAGTTCC AAC
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

reference Cleutjens, K.B., Two androgen response regions cooperate in steroid hormone regulated activity of the prostate-specific antigen promoter. J. Biol. Chem. 271 (11): 6379-6388 (1996). PMID 8626436.