RDB05542 - pKM2L-phGH1

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

pKM2L-phGH1 (#RDB05542)

Promoter Bank clone, Human growth hormone (GH1) promoter

Alternative name	p1960-1F
Clone info.	PCR amplified human growth hormone (GH1) promoter sequence was inserted into a Renilla luciferase reporter vector pKM2L (RDB04026). Genomic sequence from 4664 to 5263 of J03071.1 corresponding -499 to +101 (the transcription start site is designated +1. Fig.2 of Krawczak, M. Gene 237 (1): 143-151, 1999) is cloned. Alternatively, 63919338 to 63918738(NC_000017.11); relatively -486 to +115, where +1 corresponds to 1 nt of NM_000515.4 was cloned.
Comment	PCR cloning, forward primer, H11PB0088F: 5' agaagtggaattcaggactgaatca 3'; reverse primer, H11PB0088R: 5' gtgcccaaagggattttaggggcgc 3'; Entire sequence of promoter region has not been confirmed. Promoter activity has not been analyzed. J03071, NG_001334.
Vector backbone	pKM2L (RDB04026) (Plasmid)
Size of vector backbone	4.2 kb
Selectable markers	Kan^r
Gene/insert name	Human growth hormone 1 genomic DNA
Depositor\|Developer	DNA Bank, \|

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link]

Terms and conditions for distribution 1. The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. 2. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
Additional terms and conditions:
The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.

提供案内 (日本国内) [open/close]

Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link]
Terms and conditions for distribution	1. The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. 2. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. Additional terms and conditions: The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件	1. 本件リソースは学術研究機関にのみ提供し、学術研究にのみ利用することができる。　2. 謝辞の表明を必要とする。 MTAに書く付加的使用条件：本件リソースの使用は学術機関での学術研究に限る。本件リソースの使用にあたって、利用者はプロメガ社の限定使用条件に従う必要がある。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB05542	pKM2L-phGH1	DNA solution	JPY 9,460 (not-for-profit academic purpose) plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pKM2L-phGH1 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB05542).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB05542_A6Iup1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: M13_-40 Sequence file: RDB05542_A6Iua.seq
>05542_05542_A6Iu_M13(-40)_G04_19_ABI24.ab1 1 CAGCAGTCAG CTTGCATGCC TGCAGGTCAT TTAAATAGGG ATAACAGGGT AATGATTAAG 61 ATCTCGAGCT CTAAGCTTCA CATATGCATG CACTAGTGGC AGAAGTGGAA TTCAGGACTG 121 AATCGTGCTC ACAACCCCCA CAATCTATTG GCTGTGCTTG GCCCCTTTTC CCAACACACA 181 CATTCTGTCT GGTGGGTGGA GGTTAAACAT GCGGGGAGGA GGAAAGGGAT AGGATAGAGA 241 ATGGGATGTG GTCGGTAGGG GGTCTCAAGG ACTGGCTATC CTGACATCCT TCGCCGCGTG 301 CAGGTTGGCC ACCATGGCCT GCGGCCAGAG GGCACCCACG TGACCCTTAA AGAGAGGACA 361 AGTTGGGTGG TATCTCTGGC TGACACTCTG TGCACAACCC TCACAACACT GGTGACGGTG 421 GGAAGGGAAA GATGACAAGC CAGGGGGCAT GATCCCAGCA TGTGTGGGAG GAGCTTCTAA 481 ATTATCCATT AGCACAAGCC CGTCAGTGGC CCCATGCATA AATGTACACA GAAACAGGTG 541 GGGTCAACAG TGGGAGAGAA GGGGCCAGGG TATAAAAAGG GCCCACAAGA GACCAGCTCA 601 AGGATCCCAA GGCCCAACTC CCCGAACCAC TCAGGGTCCT GTGGACAGCT CACCTAGCTG 661 CAATGGCTAC AGGTAAGCGC CCCTAAAATC CCTTTGGGCA CGCCTGTCGA CGCGTAGAAT 721 TCACCCGGGT ACTGCAGAAG TTGGTCGTGA GGCACTGGGC AGGTAAGTAT CAAGGTTACA 781 AGACAGGTTT AAGGAGACCA ATAGAAACTG GGCTTGTCGA GACAGAGAAG ACTCTTGCGT 841 TTCTGATAGG CACCTATTGG TCTTACTGAC ATCCACTTTG CCTTTCTCTC CACAGGTGTC 901 CACTCCCAGT TCAATTACAG CTCTTAAAGG CTAGAGTACT TAAATACGAC TCACTATAGG 961 CTAGCCCACC ATGGCTTTCC AAGGTGTACG ACCCCGAGCA AACGCAAACG CATGATCACT 1021 GGGCCTCAGT GGTGGCTCGC TGCAAGCAAA TGACGTGCCT GGACTCTTTC ATCAACCTAC 1081 TATGATCGAG AGGCAACGGC CGAGAACCGG TCGATGAATT TTTTCCTGGC CAATG //
Primer: pAxCALNL_F1 Sequence file: RDB05542_A6Iub.seq
>05542_05542_A6Iu_pAxCALNL_F1_H04_22_ABI24.ab1 1 ACAAGGCTAT CTTATCATGT CTGCTCGAAG CGGCCGCTCT AGAATTACTG CTCGTTCTTC 61 AGCACGCGCT CCACGAAGCT CTTGATGTAC TTACCCATTT CATCTGGAGC GTCCTCCTGG 121 CTGAAGTGGA GGCCCTTCAC CTTCACGAAC TCGGTGTTAG GGAACTTCTT AGCTCCCTCG 181 ACAATAGCGT TGGAAAAGAA CCCAGGGTCG GACTCGATGA ACATCTTAGG CAGATCGTCG 241 CTGGCCCGAA GGTAGGCGTT GTAGTTGCGG ACAATCTGGA CGACGTCGGG CTTGCCTCCC 301 TTAACGAGAG GGATCTCGCG AGGCCAGGAG AGGGTAGGCC GTCTAACCTC GCCCTTCTCC 361 TTGAATGGCT CCAGGTAGGC AGCGAACTCC TCAGGCTCCA GTTTCCGCAT GATCTTGCTT 421 GGGAGCATGG TCTCGACGAA GAAGTTATTC TCAAGCACCA TTTTCTCGCC CTCTTCGCTC 481 TTGATCAGGG CGATATCCTC CTCGATGTCA GGCCACTCGT CCCAGGACTC GATCACGTCC 541 ACGACACTCT CAGCATGGAC GATGGCCTTG ATCTTGTCTT GGTGCTCGTA GGAGTAGTGA 601 AAGGCCAGAC AAGCCCCCCA GTCGTGGCCC ACAAAGATGA TTTTCTTTGG AAGGTTCAGC 661 AGCTCGAACC AAGCGGTGAG GTACTTGTAG TGATCCAGGA GGCGATATGA GCCATTCCCG 721 CTCTTGCCGG ACTTACCCAT TCCGATCAGA TCAGGGATGA TGCATCTAGC CACGGGCTCG 781 ATGTGAGGCA CGACGTGCCT CCACAGGTAG CTGGAGGCAG CGTTACCATG CAGAAAAATC 841 ACGGCGTTCT CGGCGTGCTT CTCGGAATCA TAGTAGTTGA TGAAGGAGTC CAGCACGTTC 901 ATTTGCTTGC AGCGAGCCCA CCACTGAGGC CCAGTGATCA TGCGTTTGCG TTGCTCGGGG 961 TCGTACACCT TGGAAGCCAT GGTGGCTAGC CCTATAGTGA GTCGTATTAA GTACTCTAGC 1021 CTTAGAGCTG TAATTTGAAC TGGGGAGTGA CACCTGTGGA GAGAAAGCAA AGTGAATGTC 1081 AGTAGACCAT TAGTGCTATC AGAACGGCAA GAGTCACTCT GATCTCGACA AGGCCCCAGA 1141 G //