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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pKM2L-phGH1

Promoter Bank clone, Human growth hormone (GH1) promoter

Catalog number RDB05542
Resource name pKM2L-phGH1
Alternative name p1960-1F
Clone info. PCR amplified human growth hormone (GH1) promoter sequence was inserted into a Renilla luciferase reporter vector pKM2L (RDB04026). Genomic sequence from 4664 to 5263 of J03071.1 corresponding -499 to +101 (the transcription start site is designated +1. Fig.2 of Krawczak, M. Gene 237 (1): 143-151, 1999) is cloned. Alternatively, 63919338 to 63918738(NC_000017.11); relatively -486 to +115, where +1 corresponds to 1 nt of NM_000515.4 was cloned.
Comment PCR cloning, forward primer, H11PB0088F: 5' agaagtggaattcaggactgaatca 3'; reverse primer, H11PB0088R: 5' gtgcccaaagggattttaggggcgc 3'; Entire sequence of promoter region has not been confirmed. Promoter activity has not been analyzed. J03071, NG_001334.
Vector backbone pKM2L (RDB04026) (Plasmid)
Size of vector backbone 4.2 kb
Selectable markers Kan^r
Gene/insert name Human growth hormone 1 genomic DNA
Depositor|Developer DNA Bank, |
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External Database
Human growth hormone 1
          Reference sequence
            

          Distribution information

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          RDB05542 pKM2L-phGH1 DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB05542_A6Iup1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: M13_-40
          Sequence file: RDB05542_A6Iua.seq check
          >05542_05542_A6Iu_M13(-40)_G04_19_ABI24.ab1
              1 CAGCAGTCAG CTTGCATGCC TGCAGGTCAT TTAAATAGGG ATAACAGGGT AATGATTAAG
             61 ATCTCGAGCT CTAAGCTTCA CATATGCATG CACTAGTGGC AGAAGTGGAA TTCAGGACTG
            121 AATCGTGCTC ACAACCCCCA CAATCTATTG GCTGTGCTTG GCCCCTTTTC CCAACACACA
            181 CATTCTGTCT GGTGGGTGGA GGTTAAACAT GCGGGGAGGA GGAAAGGGAT AGGATAGAGA
            241 ATGGGATGTG GTCGGTAGGG GGTCTCAAGG ACTGGCTATC CTGACATCCT TCGCCGCGTG
            301 CAGGTTGGCC ACCATGGCCT GCGGCCAGAG GGCACCCACG TGACCCTTAA AGAGAGGACA
            361 AGTTGGGTGG TATCTCTGGC TGACACTCTG TGCACAACCC TCACAACACT GGTGACGGTG
            421 GGAAGGGAAA GATGACAAGC CAGGGGGCAT GATCCCAGCA TGTGTGGGAG GAGCTTCTAA
            481 ATTATCCATT AGCACAAGCC CGTCAGTGGC CCCATGCATA AATGTACACA GAAACAGGTG
            541 GGGTCAACAG TGGGAGAGAA GGGGCCAGGG TATAAAAAGG GCCCACAAGA GACCAGCTCA
            601 AGGATCCCAA GGCCCAACTC CCCGAACCAC TCAGGGTCCT GTGGACAGCT CACCTAGCTG
            661 CAATGGCTAC AGGTAAGCGC CCCTAAAATC CCTTTGGGCA CGCCTGTCGA CGCGTAGAAT
            721 TCACCCGGGT ACTGCAGAAG TTGGTCGTGA GGCACTGGGC AGGTAAGTAT CAAGGTTACA
            781 AGACAGGTTT AAGGAGACCA ATAGAAACTG GGCTTGTCGA GACAGAGAAG ACTCTTGCGT
            841 TTCTGATAGG CACCTATTGG TCTTACTGAC ATCCACTTTG CCTTTCTCTC CACAGGTGTC
            901 CACTCCCAGT TCAATTACAG CTCTTAAAGG CTAGAGTACT TAAATACGAC TCACTATAGG
            961 CTAGCCCACC ATGGCTTTCC AAGGTGTACG ACCCCGAGCA AACGCAAACG CATGATCACT
           1021 GGGCCTCAGT GGTGGCTCGC TGCAAGCAAA TGACGTGCCT GGACTCTTTC ATCAACCTAC
          1081 TATGATCGAG AGGCAACGGC CGAGAACCGG TCGATGAATT TTTTCCTGGC CAATG
          //
          Primer: pAxCALNL_F1
          Sequence file: RDB05542_A6Iub.seq check
          >05542_05542_A6Iu_pAxCALNL_F1_H04_22_ABI24.ab1
              1 ACAAGGCTAT CTTATCATGT CTGCTCGAAG CGGCCGCTCT AGAATTACTG CTCGTTCTTC
             61 AGCACGCGCT CCACGAAGCT CTTGATGTAC TTACCCATTT CATCTGGAGC GTCCTCCTGG
            121 CTGAAGTGGA GGCCCTTCAC CTTCACGAAC TCGGTGTTAG GGAACTTCTT AGCTCCCTCG
            181 ACAATAGCGT TGGAAAAGAA CCCAGGGTCG GACTCGATGA ACATCTTAGG CAGATCGTCG
            241 CTGGCCCGAA GGTAGGCGTT GTAGTTGCGG ACAATCTGGA CGACGTCGGG CTTGCCTCCC
            301 TTAACGAGAG GGATCTCGCG AGGCCAGGAG AGGGTAGGCC GTCTAACCTC GCCCTTCTCC
            361 TTGAATGGCT CCAGGTAGGC AGCGAACTCC TCAGGCTCCA GTTTCCGCAT GATCTTGCTT
            421 GGGAGCATGG TCTCGACGAA GAAGTTATTC TCAAGCACCA TTTTCTCGCC CTCTTCGCTC
            481 TTGATCAGGG CGATATCCTC CTCGATGTCA GGCCACTCGT CCCAGGACTC GATCACGTCC
            541 ACGACACTCT CAGCATGGAC GATGGCCTTG ATCTTGTCTT GGTGCTCGTA GGAGTAGTGA
            601 AAGGCCAGAC AAGCCCCCCA GTCGTGGCCC ACAAAGATGA TTTTCTTTGG AAGGTTCAGC
            661 AGCTCGAACC AAGCGGTGAG GTACTTGTAG TGATCCAGGA GGCGATATGA GCCATTCCCG
            721 CTCTTGCCGG ACTTACCCAT TCCGATCAGA TCAGGGATGA TGCATCTAGC CACGGGCTCG
            781 ATGTGAGGCA CGACGTGCCT CCACAGGTAG CTGGAGGCAG CGTTACCATG CAGAAAAATC
            841 ACGGCGTTCT CGGCGTGCTT CTCGGAATCA TAGTAGTTGA TGAAGGAGTC CAGCACGTTC
            901 ATTTGCTTGC AGCGAGCCCA CCACTGAGGC CCAGTGATCA TGCGTTTGCG TTGCTCGGGG
            961 TCGTACACCT TGGAAGCCAT GGTGGCTAGC CCTATAGTGA GTCGTATTAA GTACTCTAGC
           1021 CTTAGAGCTG TAATTTGAAC TGGGGAGTGA CACCTGTGGA GAGAAAGCAA AGTGAATGTC
           1081 AGTAGACCAT TAGTGCTATC AGAACGGCAA GAGTCACTCT GATCTCGACA AGGCCCCAGA
          1141 G
          //

          Please visit Sequencing and PCR primers for primer information.

          References

          Original, user report and related articles

          reference Krawczak, M., Evolution of the proximal promoter region of the mammalian growth hormone gene. Gene 237 (1): 143-151 (1999). PMID 10524245.

          2022.05.18

          GNP_filter3_RDBDEP_html_220516.pl