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pKM2L-phCRHR1 (#RDB05536)

Promoter Bank clone, Human corticotropin releasing hormone (factor) receptor 1 (CRHR1/CRFR1) promoter

Alternative name p1954-3F
Clone info. PCR amplified human corticotropin releasing hormone (factor) receptor 1 (CRHR1/CRFR1) promoter sequence was inserted into a Renilla luciferase reporter vector pKM2L (RDB04026). Cloned fragment: 45782271 to 45784435(NC_000017.11); relatively -2049 to +116, where +1 corresponds to 1 nt of NM_004382.5.
Comment PCR cloning, forward primer, 1954 F3: 5' gccacccctcacacttgagcatcca 3'; reverse primer, 1954 R3: 5' tcggtcgctgcctggtgaggagat 3' Entire sequence of promoter region has not been confirmed.
Vector backbone pKM2L (RDB04026) (Plasmid)
Size of vector backbone 4.2 kb
Selectable markers Kan^r
Gene/insert name Human corticotropin releasing hormone receptor 1 genomic DNA
Depositor|Developer DNA Bank, |
 

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The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
提供案内 (日本国内) [open/close]

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Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB05536 pKM2L-phCRHR1 Under QC test. Please contact us. DNA solution

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How to cite this biological resource

Materials & Methods section:

The pKM2L-phCRHR1 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB05536).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

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References

Original, user report and related articles