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pKM2L-phAFP (#RDB05532)

Promoter Bank clone, Human alpha-fetoprotein (AFP) promoter

Alternative name p1949-23F
Clone info. PCR amplified human alpha-fetoprotein (AFP) promoter sequence was inserted into a Renilla luciferase reporter vector pKM2L (RDB04026). Genomic sequence from 98221 to 102066 of AC108157.3 corresponding -3846 to +32 (the transcription start site is designated +1. Fig.5 of Watanabe, K. J. Biol. Chem. 262 (10): 4812-4818, 1987 and Fig.2 of Sakai, M. J. Biol. Chem. 260 (8): 5055-5060, 1985) is cloned. Alternatively, 73432373 to 73436250(NC_000004.12); relatively -3846 to +32, where +1 corresponds to 1 nt of NM_001134.2 was cloned.
Comment PCR cloning, forward primer, 1949 F4: 5' gctcctctttattgcttgtcttgg 3'; reverse primer, 1949 R4: 5' ttttgttattggcagtggtggaa 3' Entire sequence of promoter region has not been confirmed.
Vector backbone pKM2L (RDB04026) (Plasmid)
Size of vector backbone 4.2 kb
Selectable markers Kan^r
Gene/insert name Human alpha-fetoprotein genomic DNA
Depositor|Developer DNA Bank, |
 

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RDB05532 pKM2L-phAFP DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pKM2L-phAFP was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB05532).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB13693_A5Jlp1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: M13_-40
Sequence file: RDB13693_A5Jla.seq check
>05532_13693_A5Jl_M13(-40)_D07_10.ab1
    1 CGGCGGCAGC TTGCATGCCT GCAGGTCATT TAAATAGGGA TAACAGGGTA ATGATTAAGA
   61 TCTCGAGCTC TAAGCTTCAC ATATGCATGC ACTAGTGGCG CTCCTCTTTA TTGCTTGTCT
  121 TGGAAAATTT GCTGTTCTTC ATGGTTTCTC TTTTCACTGC TATCTATTTT TCTCAACCAC
  181 TCACATGGCT ACAATAACTG TCTGCAAGCT TATGATTCCC AAATATCTAT CTCTAGCCTC
  241 AATCTTGTTC CAGAAGATAA AAAGTAGTAT TCAAATGCAC ATCAACGTCT CCACTTGGAG
  301 GGCTTAAAGA CGTTTCAACA TACAAACCGG GGAGTTTTGC CTGGAATGTT TCCTAAAATG
  361 TGTCCTGTAG CACATAGGGT CCTCTTGTTC CTTAAAATCT AATTACTTTT AGCCCAGTGC
  421 TCATCCCACC TATGGGGAGA TGAGAGTGAA AAGGGAGCCT GATTAATAAT TACACTAAGT
  481 CAATAGGCAT AGAGCCAGGA CTGTTTGGGT AAACTGGTCA CTTTATCTTA AACTAAATAT
  541 ATCCAAAACT GAACATGTAC TTAGTTACTA AGTCTTTGAC TTTATCTCAT TCATACCACT
  601 CAGCTTTATC CAGGCCACTT ATTTGACAGT ATTATTGCGA AAACTTCCTA ACTGGTCTCC
  661 TTATCATAGT CTTATCCCCT TTTGAAACAA AAGAGACAGT TTCAAAATAC AAATATGATT
  721 TTTATTAGCT CCCTTTTGTT GTCTATAATA GTCCCAGAAG GAGTTATAAA CTCCATTTAA
  781 AAAGTCTTTG AGATGTGGCC CTTGCCAACT TTGCCAGGAA TTCCCAATAT CTAGTATTTT
  841 CTACTATTAA ACTTTGTGCC TCTTCAAAAC TGCATTTTCT CTCATTCCCT AAGTGTGCAT
  901 TGTTTTCCCT TACCGGGTTG GTTTTTTCCA CCACCTTTTT ACATTTTTCC TGGAACACTA
  961 TACCCTCCCT CTTTCATTTT GGCCCACCTC TAATTTTTCT TTCAGATCTC CATGAAAGAT
 1021 GTTACTTCCC TCCAGGAAGC CCTTTATCTG ACCCCTCCAA AGATGTCATG AGTTCCCTCA
1081 TATCATCTAC TAATTCACCA GCCAATCCAA TCAACACCCA TGATTTGGGG TGAT
//
Primer: phRLR2
Sequence file: RDB13693_A5Jlb.seq check
>05532_13693_A5Jl_phRLR2_E07_13.ab1
    1 ACTTCCGTCT GAAGCCCAGT TTCTATTGGT CTCCTTAAAC CTGTCTTGTA ACCTTGATAC
   61 TTACCTGCCC AGTGCCTCAC GACCAACTTC TGCAGTACCC GGGTGAATTC TACGCGTCGA
  121 CAGGCTTTTG TTATTGGCAG TGGTGGAAGC ACAATATGGA AAATCATGCT GAAATTCTTT
  181 TATACTCTTT TCAGGCAATG CCTGTTAACT AGTAACCTTT TGTTGGTATA TCTGTTACTT
  241 TATGGTATTT CCTTAGGTTG AAAATGGGAC ATTTGCCAAT AATTAACAGC ATAGCGGTTA
  301 TTTGTATTTT ATGTTCAAGG ACACAGAGCT CTTTGGGGCA GAAAAAAAAA ACTTGCAAAC
  361 TTATTTTATT TTGCAAATAT AACAAATATT CTCCTCAAAC TACAGAATAT TCAAAGACAT
  421 GACTTGTCCC TAAGTTGCAG AATAATTAAT TTCTCTCAAA TTAAGTTAAT GAAGCCATAA
  481 AGAAAAGACA GCAGATTTGG TATGCAATTC ATCTAAATAC ATTAATTCTT TAAAACTATT
  541 AAATGCCTAT CATATGCCAG GCACACAAGA CATTGTCTGA CCAATTCAGA CTCTTCATCT
  601 TGCAAATATA ACAAATACTC TCAAAGTACA AAAAATTCAG AGAGATGACT TGTCCCTAAG
  661 TTGCAGAATA ATAAATTCAT TTCAAATTAT CATTGCTTGA TAATTATGCT GTCATTGTTT
  721 AGCTCAGAAA CACTTTTCAC TTAATATGTA TGTCAATAAG GTACATTTTA AAGTATTTAG
  781 TATGCGATGT ATGGCTTAAC ATAGAAAACT TAGAGCACAA GCCCTAATAA ACCAAGTCCT
  841 ATAATAATTT TATTTGCACA TGTGTCTTTA TAAAGTGGGT CAGGTGCATC ATTCACTTAG
  901 CAACTCAAGA TGATGATGTT AATACTAAAT GATGGATGCT AAGAGTGGCT CACCTCCTAT
  961 CATCCCTGTC TACAAGGGTT TCCCGATCTT GGCTACACAT TAGAATCACT GGGGGAACTT
 1021 TTTTGAATTT CCCAATGCCT AGTCTGTAAC CCAGACCAGT TAATCAGATC TGTGGAGTTG
1081 AGACCAGCAC AAGTATTTTT TCTAAGCTCA CAGATGCTTC CAATTAGTTT CGTAAA
//
Primer: pAxCALNL_F1
Sequence file: RDB13693_A5Jlc.seq check
>05532_13693_A5Jl_pAxCALNL_F1_F07_16.ab1
    1 ACAGTATCTT ATCATGTCTG CTCGAAGCGG CCGCTCTAGA ATTACTGCTC GTTCTTCAGC
   61 ACGCGCTCCA CGAAGCTCTT GATGTACTTA CCCATTTCAT CTGGAGCGTC CTCCTGGCTG
  121 AAGTGGAGGC CCTTCACCTT CACGAACTCG GTGTTAGGGA ACTTCTTAGC TCCCTCGACA
  181 ATAGCGTTGG AAAAGAACCC AGGGTCGGAC TCGATGAACA TCTTAGGCAG ATCGTCGCTG
  241 GCCCGAAGGT AGGCGTTGTA GTTGCGGACA ATCTGGACGA CGTCGGGCTT GCCTCCCTTA
  301 ACGAGAGGGA TCTCGCGAGG CCAGGAGAGG GTAGGCCGTC TAACCTCGCC CTTCTCCTTG
  361 AATGGCTCCA GGTAGGCAGC GAACTCCTCA GGCTCCAGTT TCCGCATGAT CTTGCTTGGG
  421 AGCATGGTCT CGACGAAGAA GTTATTCTCA AGCACCATTT TCTCGCCCTC TTCGCTCTTG
  481 ATCAGGGCGA TATCCTCCTC GATGTCAGGC CACTCGTCCC AGGACTCGAT CACGTCCACG
  541 ACACTCTCAG CATGGACGAT GGCCTTGATC TTGTCTTGGT GCTCGTAGGA GTAGTGAAAG
  601 GCCAGACAAG CCCCCCAGTC GTGGCCCACA AAGATGATTT TCTTTGGAAG GTTCAGCAGC
  661 TCGAACCAAG CGGTGAGGTA CTTGTAGTGA TCCAGGAGGC GATATGAGCC ATTCCCGCTC
  721 TTGCCGGACT TACCCATTCC GATCAGATCA GGGATGATGC ATCTAGCCAC GGGCTCGATG
  781 TGAGGCACGA CGTGCCTCCA CAGGTAGCTG GAGGCAGCGT TACCATGCAG AAAAATCACG
  841 GCGTTCTCGG CGTGCTTCTC GGAATCATAG TAGTTGATGA AGGAGTCCAG CACGTTCATT
  901 TGCTTGCAGC GAGCCCACCA CTGAGGCCCA GTGATCATGC GTTTGCGTTG CTCGGGGTCG
  961 TACACCTTGG AAGCCATGGG TGGCTAGCCC TATAGTGAGT CGTAATTAAG TACTCTAGCC
 1021 CTTTAAGAAG CTGTAATTTG AAACTGGAAG TGGAACACCT GTGGAAGAGA AAAGCAAAAG
 1081 TGCAATGTCA AGTAAGACGA ATTAGTTGCT ATCCAGAAAA ACGCAAGAAG TTCGTTCCTC
1141 TGATCCCTCC GGATCA
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

reference Watanabe, K., Cell-specific enhancer activity in a far upstream region of the human alpha-fetoprotein gene. J. Biol. Chem. 262 (10): 4812-4818 (1987). PMID 2435718.
reference Sakai, M., The human alpha-fetoprotein gene. Sequence organization and the 5' flanking region. J. Biol. Chem. 260 (8): 5055-5060 (1985). PMID 2580830.