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pKM2L-phAFP

Promoter Bank clone, Human alpha-fetoprotein (AFP) promoter

Catalog number RDB05532
Resource name pKM2L-phAFP
Alternative name p1949-23F
Clone info. PCR amplified human alpha-fetoprotein (AFP) promoter sequence was inserted into a Renilla luciferase reporter vector pKM2L (RDB04026). Genomic sequence from 98221 to 102066 of AC108157.3 corresponding -3846 to +32 (the transcription start site is designated +1. Fig.5 of Watanabe, K. J. Biol. Chem. 262 (10): 4812-4818, 1987 and Fig.2 of Sakai, M. J. Biol. Chem. 260 (8): 5055-5060, 1985) is cloned. Alternatively, 73432373 to 73436250(NC_000004.12); relatively -3846 to +32, where +1 corresponds to 1 nt of NM_001134.2 was cloned.
Comment PCR cloning, forward primer, 1949 F4: 5' gctcctctttattgcttgtcttgg 3'; reverse primer, 1949 R4: 5' ttttgttattggcagtggtggaa 3' Entire sequence of promoter region has not been confirmed.
Vector backbone pKM2L (RDB04026) (Plasmid)
Size of vector backbone 4.2 kb
Selectable markers Kan^r
Gene/insert name Human alpha-fetoprotein genomic DNA
Depositor|Developer DNA Bank, |
Other clones in our bank

External Database
Human alpha-fetoprotein

          Reference sequence
            

          Distribution information

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          RDB05532 pKM2L-phAFP DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content Promoter Collection (Renilla Luciferase) (English text)

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB13693_A5Jlp1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: M13_-40
          Sequence file: RDB13693_A5Jla.seq check
          >05532_13693_A5Jl_M13(-40)_D07_10.ab1
              1 CGGCGGCAGC TTGCATGCCT GCAGGTCATT TAAATAGGGA TAACAGGGTA ATGATTAAGA
             61 TCTCGAGCTC TAAGCTTCAC ATATGCATGC ACTAGTGGCG CTCCTCTTTA TTGCTTGTCT
            121 TGGAAAATTT GCTGTTCTTC ATGGTTTCTC TTTTCACTGC TATCTATTTT TCTCAACCAC
            181 TCACATGGCT ACAATAACTG TCTGCAAGCT TATGATTCCC AAATATCTAT CTCTAGCCTC
            241 AATCTTGTTC CAGAAGATAA AAAGTAGTAT TCAAATGCAC ATCAACGTCT CCACTTGGAG
            301 GGCTTAAAGA CGTTTCAACA TACAAACCGG GGAGTTTTGC CTGGAATGTT TCCTAAAATG
            361 TGTCCTGTAG CACATAGGGT CCTCTTGTTC CTTAAAATCT AATTACTTTT AGCCCAGTGC
            421 TCATCCCACC TATGGGGAGA TGAGAGTGAA AAGGGAGCCT GATTAATAAT TACACTAAGT
            481 CAATAGGCAT AGAGCCAGGA CTGTTTGGGT AAACTGGTCA CTTTATCTTA AACTAAATAT
            541 ATCCAAAACT GAACATGTAC TTAGTTACTA AGTCTTTGAC TTTATCTCAT TCATACCACT
            601 CAGCTTTATC CAGGCCACTT ATTTGACAGT ATTATTGCGA AAACTTCCTA ACTGGTCTCC
            661 TTATCATAGT CTTATCCCCT TTTGAAACAA AAGAGACAGT TTCAAAATAC AAATATGATT
            721 TTTATTAGCT CCCTTTTGTT GTCTATAATA GTCCCAGAAG GAGTTATAAA CTCCATTTAA
            781 AAAGTCTTTG AGATGTGGCC CTTGCCAACT TTGCCAGGAA TTCCCAATAT CTAGTATTTT
            841 CTACTATTAA ACTTTGTGCC TCTTCAAAAC TGCATTTTCT CTCATTCCCT AAGTGTGCAT
            901 TGTTTTCCCT TACCGGGTTG GTTTTTTCCA CCACCTTTTT ACATTTTTCC TGGAACACTA
            961 TACCCTCCCT CTTTCATTTT GGCCCACCTC TAATTTTTCT TTCAGATCTC CATGAAAGAT
           1021 GTTACTTCCC TCCAGGAAGC CCTTTATCTG ACCCCTCCAA AGATGTCATG AGTTCCCTCA
          1081 TATCATCTAC TAATTCACCA GCCAATCCAA TCAACACCCA TGATTTGGGG TGAT
          //
          Primer: phRLR2
          Sequence file: RDB13693_A5Jlb.seq check
          >05532_13693_A5Jl_phRLR2_E07_13.ab1
              1 ACTTCCGTCT GAAGCCCAGT TTCTATTGGT CTCCTTAAAC CTGTCTTGTA ACCTTGATAC
             61 TTACCTGCCC AGTGCCTCAC GACCAACTTC TGCAGTACCC GGGTGAATTC TACGCGTCGA
            121 CAGGCTTTTG TTATTGGCAG TGGTGGAAGC ACAATATGGA AAATCATGCT GAAATTCTTT
            181 TATACTCTTT TCAGGCAATG CCTGTTAACT AGTAACCTTT TGTTGGTATA TCTGTTACTT
            241 TATGGTATTT CCTTAGGTTG AAAATGGGAC ATTTGCCAAT AATTAACAGC ATAGCGGTTA
            301 TTTGTATTTT ATGTTCAAGG ACACAGAGCT CTTTGGGGCA GAAAAAAAAA ACTTGCAAAC
            361 TTATTTTATT TTGCAAATAT AACAAATATT CTCCTCAAAC TACAGAATAT TCAAAGACAT
            421 GACTTGTCCC TAAGTTGCAG AATAATTAAT TTCTCTCAAA TTAAGTTAAT GAAGCCATAA
            481 AGAAAAGACA GCAGATTTGG TATGCAATTC ATCTAAATAC ATTAATTCTT TAAAACTATT
            541 AAATGCCTAT CATATGCCAG GCACACAAGA CATTGTCTGA CCAATTCAGA CTCTTCATCT
            601 TGCAAATATA ACAAATACTC TCAAAGTACA AAAAATTCAG AGAGATGACT TGTCCCTAAG
            661 TTGCAGAATA ATAAATTCAT TTCAAATTAT CATTGCTTGA TAATTATGCT GTCATTGTTT
            721 AGCTCAGAAA CACTTTTCAC TTAATATGTA TGTCAATAAG GTACATTTTA AAGTATTTAG
            781 TATGCGATGT ATGGCTTAAC ATAGAAAACT TAGAGCACAA GCCCTAATAA ACCAAGTCCT
            841 ATAATAATTT TATTTGCACA TGTGTCTTTA TAAAGTGGGT CAGGTGCATC ATTCACTTAG
            901 CAACTCAAGA TGATGATGTT AATACTAAAT GATGGATGCT AAGAGTGGCT CACCTCCTAT
            961 CATCCCTGTC TACAAGGGTT TCCCGATCTT GGCTACACAT TAGAATCACT GGGGGAACTT
           1021 TTTTGAATTT CCCAATGCCT AGTCTGTAAC CCAGACCAGT TAATCAGATC TGTGGAGTTG
          1081 AGACCAGCAC AAGTATTTTT TCTAAGCTCA CAGATGCTTC CAATTAGTTT CGTAAA
          //
          Primer: pAxCALNL_F1
          Sequence file: RDB13693_A5Jlc.seq check
          >05532_13693_A5Jl_pAxCALNL_F1_F07_16.ab1
              1 ACAGTATCTT ATCATGTCTG CTCGAAGCGG CCGCTCTAGA ATTACTGCTC GTTCTTCAGC
             61 ACGCGCTCCA CGAAGCTCTT GATGTACTTA CCCATTTCAT CTGGAGCGTC CTCCTGGCTG
            121 AAGTGGAGGC CCTTCACCTT CACGAACTCG GTGTTAGGGA ACTTCTTAGC TCCCTCGACA
            181 ATAGCGTTGG AAAAGAACCC AGGGTCGGAC TCGATGAACA TCTTAGGCAG ATCGTCGCTG
            241 GCCCGAAGGT AGGCGTTGTA GTTGCGGACA ATCTGGACGA CGTCGGGCTT GCCTCCCTTA
            301 ACGAGAGGGA TCTCGCGAGG CCAGGAGAGG GTAGGCCGTC TAACCTCGCC CTTCTCCTTG
            361 AATGGCTCCA GGTAGGCAGC GAACTCCTCA GGCTCCAGTT TCCGCATGAT CTTGCTTGGG
            421 AGCATGGTCT CGACGAAGAA GTTATTCTCA AGCACCATTT TCTCGCCCTC TTCGCTCTTG
            481 ATCAGGGCGA TATCCTCCTC GATGTCAGGC CACTCGTCCC AGGACTCGAT CACGTCCACG
            541 ACACTCTCAG CATGGACGAT GGCCTTGATC TTGTCTTGGT GCTCGTAGGA GTAGTGAAAG
            601 GCCAGACAAG CCCCCCAGTC GTGGCCCACA AAGATGATTT TCTTTGGAAG GTTCAGCAGC
            661 TCGAACCAAG CGGTGAGGTA CTTGTAGTGA TCCAGGAGGC GATATGAGCC ATTCCCGCTC
            721 TTGCCGGACT TACCCATTCC GATCAGATCA GGGATGATGC ATCTAGCCAC GGGCTCGATG
            781 TGAGGCACGA CGTGCCTCCA CAGGTAGCTG GAGGCAGCGT TACCATGCAG AAAAATCACG
            841 GCGTTCTCGG CGTGCTTCTC GGAATCATAG TAGTTGATGA AGGAGTCCAG CACGTTCATT
            901 TGCTTGCAGC GAGCCCACCA CTGAGGCCCA GTGATCATGC GTTTGCGTTG CTCGGGGTCG
            961 TACACCTTGG AAGCCATGGG TGGCTAGCCC TATAGTGAGT CGTAATTAAG TACTCTAGCC
           1021 CTTTAAGAAG CTGTAATTTG AAACTGGAAG TGGAACACCT GTGGAAGAGA AAAGCAAAAG
           1081 TGCAATGTCA AGTAAGACGA ATTAGTTGCT ATCCAGAAAA ACGCAAGAAG TTCGTTCCTC
          1141 TGATCCCTCC GGATCA
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          reference Watanabe, K., Cell-specific enhancer activity in a far upstream region of the human alpha-fetoprotein gene. J. Biol. Chem. 262 (10): 4812-4818 (1987). PMID 2435718.
          reference Sakai, M., The human alpha-fetoprotein gene. Sequence organization and the 5' flanking region. J. Biol. Chem. 260 (8): 5055-5060 (1985). PMID 2580830.

          2022.05.18

          GNP_filter3_RDBDEP_html_220516.pl