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pKM2L-phIGNA2 (#RDB05527)

Promoter Bank clone, Human integrin alpha 2 subunit (ITGA2) promoter

Alternative name p1942-2F
Clone info. PCR amplified human integrin alpha 2 subunit (ITGA2) promoter sequence was inserted into a Renilla luciferase reporter vector pKM2L (RDB04026). Cloned fragment: 52988380 to 52989468(NC_000005.10); relatively -946 to +143, where +1 corresponds to 1 nt of NM_002203.3.
Comment PCR cloning, forward primer, F9PB0070F: 5' ggtttcagcaatgtgctggaaatttg 3'; reverse primer, F9PB0070R: 5' cctgggtctgaccgggggagcgt 3' Entire sequence of promoter region has not been confirmed.
Vector backbone pKM2L (RDB04026) (Plasmid)
Size of vector backbone 4.2 kb
Selectable markers Kan^r
Gene/insert name Human integrin, alpha 2 genomic DNA
Depositor|Developer DNA Bank, |
 

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RDB05527 pKM2L-phIGNA2 DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pKM2L-phIGNA2 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB05527).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB12780_A4Dp.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: M13_-40
Sequence file: RDB12780_A4Dpa.seq check
>05527_12780_A4Dp_M13(-40)_A08_02.ab1
    1 AAGCCGGTGC AGCTTGCATG CCTGCAGGTC ATTTAAATAG GGATAACAGG GTAATGATTA
   61 AGATCTCGAG CTCTAAGCTT CACATATGCA TGCACTAGTG GCGGTTTCAG CAATGTGCTG
  121 GAAATTTGTG GCCAAACTTT TGGATAAAAC ATAAGATTCA GACAAGAAAA ATTAATATCC
  181 TATTTCTTAG GTTTTTAACT AAAAATCTCG GATATAGAAT GTTCTCTTTC CAGATGTGAG
  241 ACTGAAATAA TGGAAGAAAA TGGTCACAGT CTATGTCACG GTGTAATGTA GACAGGGAGA
  301 GTCACAAGTG CATGTATGGG CTCTCAGCCT TTTCTCTGGC CTTGAAGTTA ATCTCCTAAA
  361 CTCAGTCATT AAATCAGCTC TTAATTATCT CGGAGGAGAG GAAGTAATGA TGATTTCTCT
  421 CTCTCTGTCT CTCTGTCACA CACACACACA CACACACAGC TCTTGCAGCA GGTATTGCTT
  481 AAATATCACC TTGGATAATC ATAACTTGTG AGCAGATCTT CTTTCCTGAT GAGTTAGTTT
  541 CTAACCTGGT CATTCTGCGC TTATTTTTGT CCCTTTCTCC ACCCACTTAG GAAAAACAGA
  601 GAAAGGGACG CACCGCGCAG CCCCTAGGCA CTGTGGTTTA GGGCTAGTGC CCTCGGCACC
  661 CGCTGCCAGG AGCCGGGCGC TGCCAAGGGC TGCGGAGGGG CCACGTTCTC CCGGGGACTG
  721 GGGCATCTCC TGCGTGCTGG CGACAGGCTG CGGGGGCGGA GTGGTGCCAG GGCGGGCGCT
  781 CGCCCGTCGG ATATGCCCAC CCGTCCCGTC CAGGGCAGGA AAGCCTGCCA GGGCGCCATC
  841 CCCATCCCCA CCGCCTCCAG GCTGCCGGGG CTGGGCCGCT GTACGGGAGC CAAGGTGCGG
  901 TGCCCCGCGT GTGGACGAGC CGAGGTGCAG CCCGCGGGGC CGCAGGGCCG GGGTGGGGCG
  961 GGGCGCGGCC GGAGCAGATC CGGTGTTTGC GGAATCAGGA GGGGCGGGCC GGGGCGGGCC
 1021 CTCGGCGCTG CAGGAGCTGC CCAGAAACTT TTCCCTGCTC TCACGGGCGG GGGAGAGAGC
 1081 CCTCTGGACA GCTTCTAGAG TGTGCAGTTC TCGTATCCTC GGCAGGTATC TCTGCACTCT
 1141 GCACAGGCAC TACCGTTCCC CCGGTCGGAC CAGGTCTGTT CGACGCGTAG AAATTTCATC
1201 G
//
Primer: phRLR2
Sequence file: RDB12780_A4Dpb.seq check
>05527_12780_A4Dp_phRLR2_B08_05.ab1
    1 ACCTTCTTTC CGTCTCGGAA AGCCCAGTTT CTATTGGTCT CCTTAAACCT GTCTTGTAAC
   61 CTTGATACTT ACCTGCCCAG TGCCTCACGA CCAACTTCTG CAGTACCCGG GTGAATTCTA
  121 CGCGTCGACA GGCCCTGGGT CTGACGGGGG GACGGTAGTT GCGCTGGGTT TGCAGAGGTT
  181 TGCAGAGGAT ACCCTTGGCC GAGGGATACG AGAACCTGCA CACTCTAGAA GCTGTCCAGA
  241 GGGCTTCTCT CCCCCGCCCG GTGAGAGCAG GGAAAAGTTT CTGGGCAGCT CCTGCAGCGC
  301 CGAGGGCCCG CCCCGGCCCG CCCCTCCTGA TTCCGCAAAC ACCGGATCTG CTCCGGCCGC
  361 GCCCCGCCCC ACCCCGGCCC TGCGGCCCCG CGGGCTGCAC CTCGGCTCGT CCACACGCGG
  421 GGCACCGCAC CTTGGCTCCC GTACAGCGGC CCAGCCCCGG CAGCCTGGAG GCGGTGGGGA
  481 TGGGGATGGC GCCCTGGCAG GCTTTCCTGC CCTGGACGGG ACGGGTGGGC ATATCCGACG
  541 GGCGAGCGCC CGCCCTGGCA CCACTCCGCC CCCGCAGCCT GTCGCCAGCA CGCAGGAGAT
  601 GCCCCAGTCC CCGGGAGAAC GTGGCCCCTC CGCAGCCCTT GGCAGCGCCC GGCTCCTGGC
  661 AGCGGGTGCC GAGGGCACTA GCCCTAAACC ACAGTGCCTA GGGGCTGCGC GGTGCGTCCC
  721 TTTCTCTGTT TTTCCTAAGT GGGTGGAGAA AGGGACAAAA ATAAGCGCAG AATGACCAGG
  781 TTAGAAACTA ACTCATCAGG AAAGAAGATC TGCTCACAAG TTATGATTAT CCAAGGTGAT
  841 ATTTAAGCAA TACCTGCTGC AAGAGCTGTG TGTGTGTGTG TGTGTGTGAC AGAGAGACAG
  901 AGAGAGAGAA ATCATCATTA CTTCCTCTCC TCCGAGATAA TTAAGAGCTG ATTTAATGAC
  961 TGAGTTTAGG AGATTAACTT CAAGGCCAGA GAAAAGGCTG AGAGCCCATA CATGCCACTT
 1021 GTGACTCTCC CTGTCTACAT TACACCGTGA CATAGACTGT GACCATTTTC TTCCATTATT
 1081 TCAGTCTCAC ATCTGGAAAG AAGAACATTC TATATCCGAG ATTTTTAGTA AAACCCTAAG
 1141 AAAATAGGAT ATATTTTCTT GTCTGAATCC TAATGTTTTA TCAAGTTGTC CACAAATTTC
1201 CAGCCACACA TGTTGCCC
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles