RDB05526 - pKM2L-phIL1B

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pKM2L-phIL1B (#RDB05526)

Promoter Bank clone, Human interleukin 1 beta (IL1B) promoter

Alternative name	p1941-3F
Clone info.	PCR amplified human interleukin 1 beta (IL1B) promoter sequence was inserted into a Renilla luciferase reporter vector pKM2L (RDB04026). Genomic sequence from 55967 to 52177 (reverse direction) of AC09753.7 corresponding -3757 to +13 (the transcription start site is designated +1. Fig. 1 of Shirakawa, F. Mol. Cell. Biol 13 (3) 1332-1344, 1993 and Fig.1 of Kominato, Y. Mol. Cell. Biol. 15 (1) 58-68, 1995) is cloned. Alternatively, 112840557 to 112836767(NC_000002.12); relatively -3778 to +13, where +1 corresponds to 1 nt of NM_000576.2 was cloned.
Comment	PCR cloning, forward primer, 1941 F2: 5' tctagaccagggaggagaatggaatg 3'; reverse primer, E9PB0069R: 5' gaagaggtttggtatctgccagtttc 3'; Entire sequence of promoter region has not been confirmed.
Vector backbone	pKM2L (RDB04026) (Plasmid)
Size of vector backbone	4.2 kb
Selectable markers	Kan^r
Gene/insert name	Human interleukin 1 beta (IL1B) genomic DNA
Depositor\|Developer	DNA Bank, \|

Additional map
Remarks, protocol and/or map (pdf)	RDB05526.pdf

Distribution information

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Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link]
Terms and conditions for distribution	1. The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. 2. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. Additional terms and conditions: The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件	1. 本件リソースは学術研究機関にのみ提供し、学術研究にのみ利用することができる。　2. 謝辞の表明を必要とする。 MTAに書く付加的使用条件：本件リソースの使用は学術機関での学術研究に限る。本件リソースの使用にあたって、利用者はプロメガ社の限定使用条件に従う必要がある。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB05526	pKM2L-phIL1B	DNA solution	JPY 9,460 (not-for-profit academic purpose) plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pKM2L-phIL1B was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB05526).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB12399_A5Iop1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: M13_-40 Sequence file: RDB12399_A5Ioa.seq
>05526_12399_A5Io_2_M13(-40)_G12_21.ab1 1 AGGCGGCGCA GCTTGCATGC CTGCAGGTCA TTTAATAGGG ATAACAGGGT AATGATTAAG 61 ATCTCGAGCT CTAAGCTTCA CATATGCATG CACTAGTGGC TCTAGACCAG GGAGGAGAAT 121 GGAATGTTCC TTGGACTCTG CATGTCCCCA ATCTGAGAAC CTGGATCCAA GAGGGAGAAG 181 AAGCCCATTG GAGATGATGC CATAAAGGAA GTGGAAGCGA TATGATAAAA ATCATAGTGC 241 CCATTCCCAA ATAATCCCAG AAGCAGAAGG GAAAGGAGAG AAATATCCAC AAAGACAGGT 301 GTGGGTACAC ACAACATTTT TCATACTTTA AGATCCCAGA GGGACTCATG GAAATGATAC 361 AAGAAAATGA CTCATAAGAA CAAATATTAG GAAGCCAGTG CCAAGAATGA GATGGGAAAT 421 TGGGGAAAAT GTTGGGGGCA GATTGCTTAG TTCTGTTCTA AGCAAGAGGG TGAACAAGGA 481 AGGAACAGCT CACTACAAAG AACAGACATC ACTGCATGTA CACACAATAA TATAAGAACT 541 AACCCATGAT TATTTTGCTT GTCTTCTTGT TCAAAATGAT TGAAGACCAA TGAGATGAGA 601 TCAACCTTGA TAACTGGCTG CGAAGCCCAT GATTAGACAC AAGATGGTAT CAGGGCACTT 661 GCTGCTTTGA ATAAATGTCA GTCTCCTGTC TTGGAAGAAT GACCTGACAG GGTAAAGAGG 721 AACTTGCAGC TGAGAAAGGC TTTAGTGACT CAAGAGCTGA ATAATTCCCC AAAAGCTGGA 781 GCATCCTGCA TTTCCAGCTC CCCATCTCTG CTTGTTCCAC TTCCTTTGGG GCTACATCAC 841 ATCTACATCA TCATCACTCT TCCACTCCCT CCCTAGTGCC AACTATGTTT ATAGCGAGAT 901 ATTTCTGCTC ATTGGGGAAT CGAGAGGAAG TGCTGTGACT TGAGCGTCTC GTGGAGACAG 961 ATCTGATACA TACGTGCACA CCTATTGACA TAGAAGTTTC ACTCTGAATG AATGATGATA 1021 ACAATTGGTC AGTTACAGAC AGATGGACAT GCCAAATTCG TTGCGATC //
Primer: phRLR2 Sequence file: RDB12399_A5Iob.seq
>05526_12399_A5Io_2_phRLR2_H12_24.ab1 1 AACTTTATTT GTCTCGAAAG CCAGTTTCTA TTGGTCTCCT TAAACCTGTC TTGTAACCTT 61 GATACTTACC TGCCCAGTGC CTCACGACCA ACTTCTGCAG TACCCGGGTG AATTCTACGC 121 GTCGACAGGC GAAGAGGTTT GGTATCTGCC AGTTTCTCCC TCGCTGTTTT TATAGCTTTC 181 AAAAGCAGAA GTAGGAGGCT GAGAAATTTC TCTGTTGAAT ACCTGATTTC ACAATCAAGT 241 TAAAGGAAAG GGGAAAAGAG TATTGGTGGA AGCTTCTTAG GGGAGGGGAC TAATAAACTG 301 AGATAATTCT CTGGTTCATG GAAGGGCAAG GAGTAGCAAA CTATGACACA TTTTGCAAAT 361 GTATCACCAT GCAAATATGC ATTGTTTTCC TGACAATCGT TGTGCAGTTG ATGTCCACAT 421 TAAAATACTG GATTTTCCCA CGTTAGAAGA ATGTTTAAAT TTAGTATATG TGGGACAAAG 481 TGGAAGACAC ACAGATTTAT ACATGCACAT ACTTTTCTTC ATTCACTTCT TTGTACTTAA 541 GTTTAGGAAT CTTCCCACTT ACAGATGGAT AAATGGGTAC AATGAAGGGC CAATAGCCCT 601 CCCTGTCTGT ATTGAGGGTG TGGGTCTCTA CCTTGGGTGC TGTTCTCTGC CTCGGGAGCT 661 CTCTGTCAAT TGCAGGAGCC TCTGAGGAGA AAATTGACCT TTCTTGGCTG GGGCAGAGAA 721 CATACGGTAT GCAGGGTTCA GGCTCCTGAC GGAGTTGGGG CAACCCTGGA GATAAGCTCA 781 CACAACCCTG CAAGACCAGG TGCTGTTACC CTAGCCAATC TCATGGATGA ACCAGATCAA 841 TGCCAGATGA GCTCTGCCTA AAATGATTTT TTTGGTGAAC TCTGAAAAGT GGAATATTGT 901 TTCTGTAAGA ATATCCATCT GAGACTCTAT CTCTTGGTAA TACCAAGAGT TATCAGTTTC 961 TCTTTAACCG AGACACCAGC AAAGTGCCTG CTCCAGGGTA CTGCCCAGGG GAGCCCTCCA 1021 TTTGTAGAAT GAATGAGAGT CCAGGTTATG ACAGTGCCTG CAGTGTAGGA CACTCCTCCT 1081 TTGCTCTTTG ACAGTCTGCA ATCAATACAC GTTTTTTTTT TTGAGACCGA TTCTCAACTC 1141 TGATCGCCCT AGGCCCTGGG GAGGTTGCCG TTGC //
Primer: pAxCALNL_F1 Sequence file: RDB12399_A5Ioc.seq
>05526_12399_A5Io_2_pAxCALNL_F1_H11_23.ab1 1 ACAATGAATC TTATCATGTC TGCTCGAAGC GGCCGCTCTA GAATTACTGC TCGTTCTTCA 61 GCACGCGCTC CACGAAGCTC TTGATGTACT TACCCATTTC ATCTGGAGCG TCCTCCTGGC 121 TGAAGTGGAG GCCCTTCACC TTCACGAACT CGGTGTTAGG GAACTTCTTA GCTCCCTCGA 181 CAATAGCGTT GGAAAAGAAC CCAGGGTCGG ACTCGATGAA CATCTTAGGC AGATCGTCGC 241 TGGCCCGAAG GTAGGCGTTG TAGTTGCGGA CAATCTGGAC GACGTCGGGC TTGCCTCCCT 301 TAACGAGAGG GATCTCGCGA GGCCAGGAGA GGGTAGGCCG TCTAACCTCG CCCTTCTCCT 361 TGAATGGCTC CAGGTAGGCA GCGAACTCCT CAGGCTCCAG TTTCCGCATG ATCTTGCTTG 421 GGAGCATGGT CTCGACGAAG AAGTTATTCT CAAGCACCAT TTTCTCGCCC TCTTCGCTCT 481 TGATCAGGGC GATATCCTCC TCGATGTCAG GCCACTCGTC CCAGGACTCG ATCACGTCCA 541 CGACACTCTC AGCATGGACG ATGGCCTTGA TCTTGTCTTG GTGCTCGTAG GAGTAGTGAA 601 AGGCCAGACA AGCCCCCCAG TCGTGGCCCA CAAAGATGAT TTTCTTTGGA AGGTTCAGCA 661 GCTCGAACCA AGCGGTGAGG TACTTGTAGT GATCCAGGAG GCGATATGAG CCATTCCCGA 721 TCTTGCCGGA CTTACCCATT CCGATCAGAT CAGGGATGAT GCATCTAGCC ACGGGCTCGA 781 TGTGAGGCAC GACGTGCCTC CACAGGTAGC TGGAGGCAGC GTTACCATGC AGAAAAATCA 841 CGGCGTTCTC GGCGTGCTTC TCGGAATCAT AGTAGTTGAT GAAGGAGTCC AGCACGTTCA 901 TTTGCTTGCA GCGAGCCCAC CACTGAGGCC CAGTGATCAT GCGTTTGCGT TGCTCGGGGT 961 CGTACACCTT GGAAGCCATG GTGGCTAGCC TATAGTGAGT CGTATTAAGT ACTCTAGCCC 1021 TTAAGAGCTG TAATTGAACT GGGGAGTGGA CACCCTGGTG GAGAGAGGCA AAGTGGATGT 1081 CAGTAGACCC ATAGGGTGCC TTATTCGAAC GCAGGAGTCA CCTCTGTTCT CGACAAGCCC 1141 AGATTCTATT TGGAATCCTC CATA //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

reference	Kominato, Y., Monocyte expression of the human prointerleukin 1 beta gene (IL1B) is dependent on promoter sequences which bind the hematopoietic transcription factor Spi-1/PU.1. Mol. Cell. Biol. 15 (1): 58-68 (1995). PMID 7799967.
reference	Shirakawa, F., The human prointerleukin 1 beta gene requires DNA sequences both proximal and distal to the transcription start site for tissue-specific induction. Mol. Cell. Biol. 13 (3): 1332-1344 (1993). PMID 8441379.
user_report	Matsushita, A., TAZ activation by Hippo pathway dysregulation induces cytokine gene expression and promotes mesothelial cell transformation. Oncogene 38: 1966–1978 (2019). PMID 30401981. [link to RRC of NBRP]