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pKM2L-phIL1B (#RDB05526)

Promoter Bank clone, Human interleukin 1 beta (IL1B) promoter


Drawn by SnapGene® software
Sequence information
(Assembled from experimentally sequenced data)		 GenBank Flat File Format open
SnapGene file download
Aditional Information
Publication Unpublished bioresource
Test sheet Data Sheet open 
 
Alternative name p1941-3F
Clone info. PCR amplified human interleukin 1 beta (IL1B) promoter sequence was inserted into a Renilla luciferase reporter vector pKM2L (RDB04026). Genomic sequence from 55967 to 52177 (reverse direction) of AC09753.7 corresponding -3757 to +13 (the transcription start site is designated +1. Fig. 1 of Shirakawa, F. Mol. Cell. Biol 13 (3) 1332-1344, 1993 and Fig.1 of Kominato, Y. Mol. Cell. Biol. 15 (1) 58-68, 1995) is cloned. Alternatively, 112840557 to 112836767(NC_000002.12); relatively -3778 to +13, where +1 corresponds to 1 nt of NM_000576.2 was cloned.
Comment PCR cloning, forward primer, 1941 F2: 5' tctagaccagggaggagaatggaatg 3'; reverse primer, E9PB0069R: 5' gaagaggtttggtatctgccagtttc 3'.
Known differences: Compared with NC_000002.12, G to A substitution at 112836810 nt, A to G substitution at 112837290 nt, CCAA deletion from 112837582 to 112837585 nt, G to A substitution at 112837842 nt, TTCT deletion from 112838817 to 112838820 nt, G to A substitution at 112839735 nt, G to A substitution at 112840530 nt.
Vector backbone pKM2L (RDB04026) (Plasmid)
Size of vector backbone 4.2 kb
Selectable markers Kan^r
Growth conditions 37℃, LB+Kan
Gene/insert name Human interleukin 1 beta (IL1B) genomic DNA
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RDB05526 pKM2L-phIL1B DNA solution

How to cite this biological resource

Materials & Methods section:

The pKM2L-phIL1B was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB05526).

Reference section:

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

reference Kominato, Y., Monocyte expression of the human prointerleukin 1 beta gene (IL1B) is dependent on promoter sequences which bind the hematopoietic transcription factor Spi-1/PU.1. Mol. Cell. Biol. 15 (1): 58-68 (1995). PMID 7799967. [PubMed]
reference Shirakawa, F., The human prointerleukin 1 beta gene requires DNA sequences both proximal and distal to the transcription start site for tissue-specific induction. Mol. Cell. Biol. 13 (3): 1332-1344 (1993). PMID 8441379. [PubMed] [Article]
user_report Matsushita, A., TAZ activation by Hippo pathway dysregulation induces cytokine gene expression and promotes mesothelial cell transformation. Oncogene 38: 1966–1978 (2019). PMID 30401981. [PubMed] [Article] [RRC of NBRP]