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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pKM2L-phIL1B

Promoter Bank clone, Human interleukin 1 beta (IL1B) promoter

Catalog number RDB05526
Resource name pKM2L-phIL1B
Alternative name p1941-3F
Clone info. PCR amplified human interleukin 1 beta (IL1B) promoter sequence was inserted into a Renilla luciferase reporter vector pKM2L (RDB04026). Genomic sequence from 55967 to 52177 (reverse direction) of AC09753.7 corresponding -3757 to +13 (the transcription start site is designated +1. Fig. 1 of Shirakawa, F. Mol. Cell. Biol 13 (3) 1332-1344, 1993 and Fig.1 of Kominato, Y. Mol. Cell. Biol. 15 (1) 58-68, 1995) is cloned. Alternatively, 112840557 to 112836767(NC_000002.12); relatively -3778 to +13, where +1 corresponds to 1 nt of NM_000576.2 was cloned.
Comment PCR cloning, forward primer, 1941 F2: 5' tctagaccagggaggagaatggaatg 3'; reverse primer, E9PB0069R: 5' gaagaggtttggtatctgccagtttc 3'; Entire sequence of promoter region has not been confirmed.
Vector backbone pKM2L (RDB04026) (Plasmid)
Size of vector backbone 4.2 kb
Selectable markers Kan^r
Gene/insert name Human interleukin 1 beta (IL1B) genomic DNA
Depositor|Developer DNA Bank, |
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External Database
Human interleukin 1 beta (IL1B)

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR 1. The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. 2. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
          Additional terms and conditions for distribution The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
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          RDB05526 pKM2L-phIL1B DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Remarks, protocol and/or map (gif) RDB05526a.gif
          Remarks, protocol and/or map (pdf) RDB05526.pdf

          Original reference

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content Dnaconda's recommendation EXR0092e (English text)
          Featured content Dnaconda's recommendation EXR0092j (Japanese text)
          Featured content Promoter Collection (Renilla Luciferase) (English text)

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB12399_A5Iop1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: M13_-40
          Sequence file: RDB12399_A5Ioa.seq check
          >05526_12399_A5Io_2_M13(-40)_G12_21.ab1
              1 AGGCGGCGCA GCTTGCATGC CTGCAGGTCA TTTAATAGGG ATAACAGGGT AATGATTAAG
             61 ATCTCGAGCT CTAAGCTTCA CATATGCATG CACTAGTGGC TCTAGACCAG GGAGGAGAAT
            121 GGAATGTTCC TTGGACTCTG CATGTCCCCA ATCTGAGAAC CTGGATCCAA GAGGGAGAAG
            181 AAGCCCATTG GAGATGATGC CATAAAGGAA GTGGAAGCGA TATGATAAAA ATCATAGTGC
            241 CCATTCCCAA ATAATCCCAG AAGCAGAAGG GAAAGGAGAG AAATATCCAC AAAGACAGGT
            301 GTGGGTACAC ACAACATTTT TCATACTTTA AGATCCCAGA GGGACTCATG GAAATGATAC
            361 AAGAAAATGA CTCATAAGAA CAAATATTAG GAAGCCAGTG CCAAGAATGA GATGGGAAAT
            421 TGGGGAAAAT GTTGGGGGCA GATTGCTTAG TTCTGTTCTA AGCAAGAGGG TGAACAAGGA
            481 AGGAACAGCT CACTACAAAG AACAGACATC ACTGCATGTA CACACAATAA TATAAGAACT
            541 AACCCATGAT TATTTTGCTT GTCTTCTTGT TCAAAATGAT TGAAGACCAA TGAGATGAGA
            601 TCAACCTTGA TAACTGGCTG CGAAGCCCAT GATTAGACAC AAGATGGTAT CAGGGCACTT
            661 GCTGCTTTGA ATAAATGTCA GTCTCCTGTC TTGGAAGAAT GACCTGACAG GGTAAAGAGG
            721 AACTTGCAGC TGAGAAAGGC TTTAGTGACT CAAGAGCTGA ATAATTCCCC AAAAGCTGGA
            781 GCATCCTGCA TTTCCAGCTC CCCATCTCTG CTTGTTCCAC TTCCTTTGGG GCTACATCAC
            841 ATCTACATCA TCATCACTCT TCCACTCCCT CCCTAGTGCC AACTATGTTT ATAGCGAGAT
            901 ATTTCTGCTC ATTGGGGAAT CGAGAGGAAG TGCTGTGACT TGAGCGTCTC GTGGAGACAG
            961 ATCTGATACA TACGTGCACA CCTATTGACA TAGAAGTTTC ACTCTGAATG AATGATGATA
          1021 ACAATTGGTC AGTTACAGAC AGATGGACAT GCCAAATTCG TTGCGATC
          //
          Primer: phRLR2
          Sequence file: RDB12399_A5Iob.seq check
          >05526_12399_A5Io_2_phRLR2_H12_24.ab1
              1 AACTTTATTT GTCTCGAAAG CCAGTTTCTA TTGGTCTCCT TAAACCTGTC TTGTAACCTT
             61 GATACTTACC TGCCCAGTGC CTCACGACCA ACTTCTGCAG TACCCGGGTG AATTCTACGC
            121 GTCGACAGGC GAAGAGGTTT GGTATCTGCC AGTTTCTCCC TCGCTGTTTT TATAGCTTTC
            181 AAAAGCAGAA GTAGGAGGCT GAGAAATTTC TCTGTTGAAT ACCTGATTTC ACAATCAAGT
            241 TAAAGGAAAG GGGAAAAGAG TATTGGTGGA AGCTTCTTAG GGGAGGGGAC TAATAAACTG
            301 AGATAATTCT CTGGTTCATG GAAGGGCAAG GAGTAGCAAA CTATGACACA TTTTGCAAAT
            361 GTATCACCAT GCAAATATGC ATTGTTTTCC TGACAATCGT TGTGCAGTTG ATGTCCACAT
            421 TAAAATACTG GATTTTCCCA CGTTAGAAGA ATGTTTAAAT TTAGTATATG TGGGACAAAG
            481 TGGAAGACAC ACAGATTTAT ACATGCACAT ACTTTTCTTC ATTCACTTCT TTGTACTTAA
            541 GTTTAGGAAT CTTCCCACTT ACAGATGGAT AAATGGGTAC AATGAAGGGC CAATAGCCCT
            601 CCCTGTCTGT ATTGAGGGTG TGGGTCTCTA CCTTGGGTGC TGTTCTCTGC CTCGGGAGCT
            661 CTCTGTCAAT TGCAGGAGCC TCTGAGGAGA AAATTGACCT TTCTTGGCTG GGGCAGAGAA
            721 CATACGGTAT GCAGGGTTCA GGCTCCTGAC GGAGTTGGGG CAACCCTGGA GATAAGCTCA
            781 CACAACCCTG CAAGACCAGG TGCTGTTACC CTAGCCAATC TCATGGATGA ACCAGATCAA
            841 TGCCAGATGA GCTCTGCCTA AAATGATTTT TTTGGTGAAC TCTGAAAAGT GGAATATTGT
            901 TTCTGTAAGA ATATCCATCT GAGACTCTAT CTCTTGGTAA TACCAAGAGT TATCAGTTTC
            961 TCTTTAACCG AGACACCAGC AAAGTGCCTG CTCCAGGGTA CTGCCCAGGG GAGCCCTCCA
           1021 TTTGTAGAAT GAATGAGAGT CCAGGTTATG ACAGTGCCTG CAGTGTAGGA CACTCCTCCT
           1081 TTGCTCTTTG ACAGTCTGCA ATCAATACAC GTTTTTTTTT TTGAGACCGA TTCTCAACTC
          1141 TGATCGCCCT AGGCCCTGGG GAGGTTGCCG TTGC
          //
          Primer: pAxCALNL_F1
          Sequence file: RDB12399_A5Ioc.seq check
          >05526_12399_A5Io_2_pAxCALNL_F1_H11_23.ab1
              1 ACAATGAATC TTATCATGTC TGCTCGAAGC GGCCGCTCTA GAATTACTGC TCGTTCTTCA
             61 GCACGCGCTC CACGAAGCTC TTGATGTACT TACCCATTTC ATCTGGAGCG TCCTCCTGGC
            121 TGAAGTGGAG GCCCTTCACC TTCACGAACT CGGTGTTAGG GAACTTCTTA GCTCCCTCGA
            181 CAATAGCGTT GGAAAAGAAC CCAGGGTCGG ACTCGATGAA CATCTTAGGC AGATCGTCGC
            241 TGGCCCGAAG GTAGGCGTTG TAGTTGCGGA CAATCTGGAC GACGTCGGGC TTGCCTCCCT
            301 TAACGAGAGG GATCTCGCGA GGCCAGGAGA GGGTAGGCCG TCTAACCTCG CCCTTCTCCT
            361 TGAATGGCTC CAGGTAGGCA GCGAACTCCT CAGGCTCCAG TTTCCGCATG ATCTTGCTTG
            421 GGAGCATGGT CTCGACGAAG AAGTTATTCT CAAGCACCAT TTTCTCGCCC TCTTCGCTCT
            481 TGATCAGGGC GATATCCTCC TCGATGTCAG GCCACTCGTC CCAGGACTCG ATCACGTCCA
            541 CGACACTCTC AGCATGGACG ATGGCCTTGA TCTTGTCTTG GTGCTCGTAG GAGTAGTGAA
            601 AGGCCAGACA AGCCCCCCAG TCGTGGCCCA CAAAGATGAT TTTCTTTGGA AGGTTCAGCA
            661 GCTCGAACCA AGCGGTGAGG TACTTGTAGT GATCCAGGAG GCGATATGAG CCATTCCCGA
            721 TCTTGCCGGA CTTACCCATT CCGATCAGAT CAGGGATGAT GCATCTAGCC ACGGGCTCGA
            781 TGTGAGGCAC GACGTGCCTC CACAGGTAGC TGGAGGCAGC GTTACCATGC AGAAAAATCA
            841 CGGCGTTCTC GGCGTGCTTC TCGGAATCAT AGTAGTTGAT GAAGGAGTCC AGCACGTTCA
            901 TTTGCTTGCA GCGAGCCCAC CACTGAGGCC CAGTGATCAT GCGTTTGCGT TGCTCGGGGT
            961 CGTACACCTT GGAAGCCATG GTGGCTAGCC TATAGTGAGT CGTATTAAGT ACTCTAGCCC
           1021 TTAAGAGCTG TAATTGAACT GGGGAGTGGA CACCCTGGTG GAGAGAGGCA AAGTGGATGT
           1081 CAGTAGACCC ATAGGGTGCC TTATTCGAAC GCAGGAGTCA CCTCTGTTCT CGACAAGCCC
          1141 AGATTCTATT TGGAATCCTC CATA
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          reference Kominato, Y., Monocyte expression of the human prointerleukin 1 beta gene (IL1B) is dependent on promoter sequences which bind the hematopoietic transcription factor Spi-1/PU.1. Mol. Cell. Biol. 15 (1): 58-68 (1995). PMID 7799967.
          reference Shirakawa, F., The human prointerleukin 1 beta gene requires DNA sequences both proximal and distal to the transcription start site for tissue-specific induction. Mol. Cell. Biol. 13 (3): 1332-1344 (1993). PMID 8441379.
          user_report Matsushita, A., TAZ activation by Hippo pathway dysregulation induces cytokine gene expression and promotes mesothelial cell transformation. Oncogene 38: 1966–1978 (2019). PMID 30401981.

          2022.05.18

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