RDB05514 - pKM2L-phMK

Resource data sheet
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pKM2L-phMK

Promoter Bank clone, Human midkine (MK) promoter


Catalog number	RDB05514
Resource name	pKM2L-phMK
Alternative name	p1928-13F
Clone info.	The Human midkine (MK) promoter sequence (PCR-amplified 2,312 bp) was inserted into a Renilla luciferase reporter vector pKM2L (RDB4026). Forward primer, H7PB0056F: 5' gatcaggggacgggatggggtac 3'; Reverse primer, 1928 R2: 5' gcttcgctccctcccgcgccgggt 3' Entire sequence of promoter region has not been confirmed.
Vector backbone	pKM2L (RDB4026) (Plasmid)
Size of vector backbone	4.2 kb
Selectable markers	Kan^r
Gene/insert name	Human midkine (neurite growth-promoting factor 2) genomic DNA
Reference of nucleotide sequence	D10604, AC116021 (DDBJ accession number)
Depositor	DNA Bank, \|
Other clones in our bank	human MDK (NCBI Gene 4192) \|

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR 1. The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. 2. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.

Additional terms and conditions for distribution The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.

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Terms and conditions set forth by the DEPOSITOR	1. The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. 2. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
Additional terms and conditions for distribution	The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
Ordering	Please visit Information of Request for Distribution. Order form [Credit Card Payment] [Bank Transfer or Check Payment] Material Transfer Agreement (MTA) [Word]

提供条件	1. 本件リソースは学術研究機関にのみ提供し、学術研究にのみ利用することができる。　2. 謝辞の表明を必要とする。
付加的提供条件	本件リソースの使用は学術機関での学術研究に限る。本件リソースの使用にあたって、利用者はプロメガ社の限定使用条件に従う必要がある。
提供依頼	手続きの詳細は、「提供申込みについて」をご覧ください。提供依頼書 [Word] 提供同意書 [Word]

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB05514	pKM2L-phMK	DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Featured content	Promoter Collection (Renilla Luciferase) (English text)

Sequence information

This clone will be sequenced a portion and digested by restriction enzyme for examination.

References

user report	Maldonado AR., Molecular engineering and validation of an oncolytic herpes simplex virus type 1 transcriptionally targeted to midkine-positive tumors. J. Gene. Med., 12, 613-623 (2010). PMID 20603890.

2018.12.10

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