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pKM2L-phMK (#RDB05514)

Promoter Bank clone, Human midkine (MK) promoter

Alternative name p1928-13F
Clone info. PCR amplified human midkine (MK) promoter sequence was inserted into a Renilla luciferase reporter vector pKM2L (RDB04026). Cloned fragment: 46379423 to 46381740(NC_000011.10); relatively -1645 to +673, where +1 corresponds to 1 nt of NM_001012334.2; relatively -2187 to +131, where +1 corresponds to 1 nt of NM_002391.4.
Comment PCR cloning forward primer, H7PB0056F: 5' gatcaggggacgggatggggtac 3'; reverse primer, 1928 R2: 5' gcttcgctccctcccgcgccgggt 3' Entire sequence of promoter region has not been confirmed.
Vector backbone pKM2L (RDB04026) (Plasmid)
Size of vector backbone 4.2 kb
Selectable markers Kan^r
Gene/insert name Human midkine (neurite growth-promoting factor 2) genomic DNA
Depositor|Developer DNA Bank, |
 

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The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
提供案内 (日本国内) [open/close]

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Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB05514 pKM2L-phMK Under QC test. Please contact us. DNA solution

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How to cite this biological resource

Materials & Methods section:

The pKM2L-phMK was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB05514).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

References

Original, user report and related articles

user_report Maldonado AR., Molecular engineering and validation of an oncolytic herpes simplex virus type 1 transcriptionally targeted to midkine-positive tumors. J. Gene. Med., 12, 613-623 (2010). PMID 20603890. [link to RRC of NBRP]