Please review the QC test results indicated by icon below as well as clone information before placing your order.

pKM2L-phP14ARF (#RDB05510)

Promoter Bank clone, Human p14 alternative reding frame (ARF) promoter

Alternative name	p1923-23F
Clone info.	PCR amplified human p14 alternative reding frame (ARF) promoter sequence was inserted into a Renilla luciferase reporter vector pKM2L (RDB04026). Genomic sequence of 4728nt to 5703nt of AF082338.1 corresponding -922 to +54 (tanscription start site is designated +1. Fig.2B of Robertson, KD., Mol. Cell. Biol. 18 (11): 6457-6473, 1998) was PCR-amplified and then cloned.
Comment	PCR cloning, forward primer, C7PB0051F: 5' agcgctcagatgctccgcggctg 3'; ｒeverse primer, C7PB0051R: 5' gagctcggcagccgctgcgccg 3'.
Vector backbone	pKM2L (RDB04026) (Plasmid)
Size of vector backbone	4.2 kb
Selectable markers	Kan^r
Gene/insert name	Human p14 alternative reding frame (ARF) genomic DNA
Depositor|Developer	DNA Bank, |

 

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1. The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. 2. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. Additional terms and conditions: The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.

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Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB05510	pKM2L-phP14ARF	DNA solution

Please review the QC test results indicated by icon below as well as clone information before placing your order.

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How to cite this biological resource

Materials & Methods section:

The pKM2L-phP14ARF was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB05510).

Reference section:

Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB05510_A8Bmp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: M13_-40 () Sequence file: RDB05510_A8Bma.seq
>05510_05510_A8Bm_M13_-40_A01_01_ABI08.ab1     1 AAGCCAGGGC AAGCTTGGCA TGCCTGCAGG TCATTTAAAT AGGGATAACA GGGTAATGAT    61 TAAGATCTCG AGCTCTAAGC TTCACATATG CATGCACTAG TGGCGGTGCT CAGATGCTCC   121 GCGGCTGTCG TGAAGGTTAA AACCGAAAAT AAAAATGGGC TAGACACAAA GGACTCGGTG   181 CTTGTCCCAG CCAGGCGCCC TCGGCGACGC GGGCAGCTGG GAGGGGAATG GGCGCCCGGA   241 CCCAGCTGGG ACCCCCGGGT GCGACTCCAC CTACCTAGTC CGGCGCCAGG CCGGGTCGAC   301 AGCTCCGGCA GCGCCAGCGC CGCGCCGTGT CCAGATGTCG CGTCAGAGGC GTGCAGCGGT   361 TTAGTTTAAT TTCGCTTGTT TTCCAAATCT AGAAGAGGAG CGGAGCGGCT TTTAGTTCAA   421 AACTGACATT CAGCCTCCTG ATTGGCGGAT AGAGCAATGA GATGACCTCG CTTTCCTTTC   481 TTCCTTTTTC ATTTTTAAAT AATCTAGTTT GAAGAATGGA AGACTTTCGA CGAGGGGAGC   541 CAGGAATAAA ATAAGGGGAA TAGGGGAGCG GGGACGCGAG CAGCACCAGA ATCCGCGGGA   601 GCGCGGCTGT TCCTGGTAGG GCCGTGTCAG GTGACGGATG TAGCTAGGGG GCGAGCTGCC   661 TGGAGTTGCG TTCCAGGCGT CCGGCCCCTG GGCCGTCACC GCGGGGCGCC CGCGCTGAGG 721 GTGGGAAGAT GGTGGTGGGG GTGGGGATCA TCTGG //
Primer: phRLR2 () Sequence file: RDB05510_A8Bmb.seq
>05510_05510_A8Bm_phRLR2_B01_02_ABI08.ab1     1 AACATTCCTT TGTTTGACAA GCCCAGTTTC TATTGGTCTC CTTAAACCTG TCTTGTAACC    61 TTGATACTTA CCTGCCCAGT GCCTCACGAC CAACTTCTGC AGTACCCGGG TGAATTCTAC   121 GCGTCGACAG GCGAGCTCGG CAGCCGCTGC GCCGCCCTTT GGCACCAGAG GTGAGCAGCG   181 CCACTCCTGC CCCCTTAACT GCAGACTGGG ACCCACGCAC CGCCCCCTGC CCATCTCCGC   241 CCCGCAGGCG CGCACCCGCC TTCCCTGAGC GCGCCCGCCC CCCACCTTCA CCCCCACCCC   301 CACCCCACCC CCACTCCCAC CCGGACCTCC AAGATCTCGG AACGGCTCTG AGCCCTGCGC   361 ACGCGGGAAG GGCTGCCGGA GGCGCCCGTA GGGAGGCGCG CGCGCGGGCG GCTCAGGGCC   421 CGCGTTCCTC TCCCTCCCGC CTACCGCCAC TTTCCCGCCC TGTGTGCGCC CCCACCCCCA   481 CCACCATCTT CCCACCCTCA GCGCGGGCGC CCCGCGGTGA CGGCCCAGGG GCCGGACGCC   541 TGGAACGCAA CTCCAGGCAG CTCGCCCCCT AGCTACATCC GTCACCTGAC ACGGCCCTAC   601 CAGGAACAGC CGCGCTCCCG CGGATTCTGG TGCTGCTCGC GTCCCCGCTC CCCTATTCCC   661 CTTATTTTAT TCCTGGCTCC CCTCGTCGAA AGTCTTCCAT TCTTCAAACT AGATTATTTA   721 AAAATGAAAA AGGAAGAAAG GAAAGCGAGG TCATCTCATT GCTCTATCCG CCAATCAGGA   781 GGCTGAATGT CAGTTTTGAA CTAAAAGCCG CTCCGCTCCT CTTCTAGATT TGGAAAACAA   841 GCGAAATTAA ACTAAACCGC TGCACGCCTC TGACGCGACA TCTGGACACG GCGCGGCGCT   901 GGCGCTGCCG GAGCTGTCGA CCCGGCCTGG CGCCGGACTA GGTAGGTGGA GTCGCACCCG   961 GGGGGTCCCA GCTGGGTCCG GGCGCCCATT CCCCTCCCAG CTGCCCGCGT CGCCGAGGGC  1021 GCCTGCTGGG ACAAGCACCG AGTCCCTTTG GTGTCTAGCC CAATTTTTAT TTTCGGTTTT  1081 AAACGTCACG GACAGCCGGC GGAGCCATCC TGAAGCACGG TCACTAGTGC AATGCCATAT 1141 GTGAAGCTTT AGAGCCTCGA AGAATCCTTA AATCCAGA //
Primer: pAxCALNL_F1 () Sequence file: RDB05510_A8Bmc.seq
>05510_05510_A8Bm_pAxCALNL_F1_C01_03_ABI08.ab1     1 AAGTGATCTT ATATGTCTGC TCGAGCGGCC GCTCTAGAAT TACTGCTCGT TCTTCAGCAC    61 GCGCTCCACG AAGCTCTTGA TGTACTTACC CATTTCATCT GGAGCGTCCT CCTGGCTGAA   121 GTGGAGGCCC TTCACCTTCA CGAACTCGGT GTTAGGGAAC TTCTTAGCTC CCTCGACAAT   181 AGCGTTGGAA AAGAACCCAG GGTCGGACTC GATGAACATC TTAGGCAGAT CGTCGCTGGC   241 CCGAAGGTAG GCGTTGTAGT TGCGGACAAT CTGGACGACG TCGGGCTTGC CTCCCTTAAC   301 GAGAGGGATC TCGCGAGGCC AGGAGAGGGT AGGCCGTCTA ACCTCGCCCT TCTCCTTGAA   361 TGGCTCCAGG TAGGCAGCGA ACTCCTCAGG CTCCAGTTTC CGCATGATCT TGCTTGGGAG   421 CATGGTCTCG ACGAAGAAGT TATTCTCAAG CACCATTTTC TCGCCCTCTT CGCTCTTGAT   481 CAGGGCGATA TCCTCCTCGA TGTCAGGCCA CTCGTCCCAG GACTCGATCA CGTCCACGAC   541 ACTCTCAGCA TGGACGATGG CCTTGATCTT GTCTTGGTGC TCGTAGGAGT AGTGAAAGGC   601 CAGACAAGCC CCCCAGTCGT GGCCCACAAA GATGATTTTC TTTGGAAGGT TCAGCAGCTC   661 GAACCAAGCG GTGAGGTACT TGTAGTGATC CAGGAGGCGA TATGAGCCAT TCCCGCTCTT   721 GCCGGACTTA CCCATTCCGA TCAGATCAGG GATGATGCAT CTAGCCACGG GCTCGATGTG   781 AGGCACGACG TGCCTCCACA GGTAGCTGGA GGCAGCGTTA CCATGCAGAA AAATCACGGC   841 GTTCTCGGCG TGCTTCTCGG AATCATAGTA GTTGATGAAA GGAGTCCAGC ACGTTCATTT   901 GCTTGCAGCG AGCCCACCAC TGAGGCCCAG TGATCATGCG TTTGCGTTGC TCGGGGGTCG   961 TACACCTTGG AAAGCCATGG GTGGGCTAGC CTATAGTGAG TCGTATTTAA AGTACTCTAG  1021 GCCTTAAGAG GCTTGTTAAT TTGAACTGGG GAGGTGGAAC ACCTGTTGGG AGAGAAAAGG  1081 CAAAGTGGGA TGTAAGTAAA GACCAATAGG TTGCTTATCA GGAACGCAGG AGTCGTCTCT 1141 GGTCTCGACA AAGCCCAGTT TCTTATTTGG TCTTTA //

Please visit Sequencing and PCR primers for primer information.

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References

Original, user report and related articles

Resource search

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Link

Other resources in our bank

• human p14 alternative reding frame (ARF)

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External Database
Human p14 alternative reding frame (ARF)

• in KEGG Gene
• in KEGG Orthology
• in NCBI Gene

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Reference sequence
  AF082338, AC000049, AL449423

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Featured content

• Promoter Collection (Renilla Luciferase) (English text)

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