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pKM2L-phCDKN2

Promoter Bank clone, Human p16 cyclin-dependent kinase inhibitor 2 (CDKN2/INK4A/CDK4I/MTS1) promoter

Catalog number RDB05509
Resource name pKM2L-phCDKN2
Alternative name p1922-5F
Clone info. PCR amplified human p16 cyclin-dependent kinase inhibitor 2 (CDKN2/INK4A/CDK4I/MTS1) promoter sequence was inserted into a Renilla luciferase reporter vector pKM2L (RDB04026). Cloned fragment: 21977927 to 21974960(NC_000009.12); relatively -2794 to +174, where +1 corresponds to 1 nt of NM_000077.4.
Comment PCR cloning, forward primer, 1922 F2: 5' atctcccatcttcttcaccaatagc 3'; reverse primer, 1922 R2: 5' gaccctgtccctcaaatcctctg 3'; Entire sequence of promoter region has not been confirmed. Promoter activity has not been analyzed. Known difference (at least) : C to T substitution at 21,975,018nt of NC_000009.12.
Vector backbone pKM2L (RDB04026) (Plasmid)
Size of vector backbone 4.2 kb
Selectable markers Kan^r
Gene/insert name Human p16 cyclin-dependent kinase inhibitor 2 (CDKN2/INK4A/CDK4I/MTS1) genomic DNA
Depositor|Developer DNA Bank, |
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External Database
Human p16 cyclin-dependent kinase inhibitor 2 (CDKN2/INK4A/CDK4I/MTS1)

          Reference sequence
            AF527803

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR 1. The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. 2. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
          Additional terms and conditions for distribution The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
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          RDB05509 pKM2L-phCDKN2 DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Sequence RDB05509z.seq

          Original reference

          Further references such as user reports and related articles (go to bottom)

          Featured content


          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB05509_A7Iep1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: M13_-40
          Sequence file: RDB05509_A7Iea.seq check
          >05509_05509_A7Ie_M13_-40_E10_13_ABI24.ab1
              1 CCGGCCGCGC CAGCTTGCAT GCCTGCAGGT CATTTAAATA GGGATAACAG GGTAATGATT
             61 AAGATCTCGA GCTCTAAGCT TCACATATGC ATGCACTAGT GGCATCTCCC ATCTTCTTCA
            121 CCAATAGCTA TATATATTTT TCACAAGAGC TGAAAGAGTT CTTGATGTAG GAATCCATGG
            181 TAGAGTTTCA GAGAAATCCC TGAATTCACT GAAAGTTTTA TCTAGAAATA CATGTGCAAG
            241 TGAACACATC TTTTTTAAAA AAAATCATTA CCTACTTTCT TTTTTGAGAA GAAGGTATTT
            301 ATTTCAACAG ACTCTTGAAG GAGCCTACTC TTCCCACTCT CCCACCCCCA TTAAGAACCA
            361 CTGTAGGCCG GGCACGATGG CTCATGCCTG TAATCCCAGC ACTTTGGGAG GCTAAGGTGG
            421 GTGGATCACC TGAGGTCAGG AGTTCGAGAC AAGCCTAGCC AACATAGTGA AACCCCGTCT
            481 CTACTAATAA TACAAAAATT AGCTGGGTAT GGCAGCATGT GCCTGTAATC CCAGCTACTC
            541 GGGAGGCTGA GGCAGGAGAA TTGCTCGAAC CCGGGAGGCG GAGGTTGCAG TGAACCGAGA
            601 GAGATCGTGC GGTGCCATTT CACTCCAGCC TGGGCAACAG AGCGAAACTC CATCTCAAAA
            661 AAACACACAA AACAAACAAA CAAAAAGAAA GAACCATTGT ATTAGTGATG GAAATGTGTT
            721 CCCTCCCTCC CATCCTGGCA ACCACTTTCT TCCTCCTCCA TCATAAAATA TCTTAAACTA
            781 AACTAAAATA ATTTTATTTA TCGATAGTTT GAATTTTCCC TATCATTGCT ACACAGCTAA
            841 TTGAGAGGTA CCCCGAGGAA AATATAAATG GTACAGTAAT GCATTGTAGA TTTTAATAAC
            901 ATACTTGACA TCCCAAATTG TTTTCATTGG CTTCATTTTA AAAAACTACA TGTTTTAAAA
            961 TCAAGCAGAC ACTAAAAGTA CAAGAATAAT ACTGGGTCTA CAAGGTTTTA AGTCAACCAG
           1021 GGATTGAAAT ATAACTTTTA ACAGAGCTGG GATTATCCAG TAGCAGATTA AGCATGTGCT
           1081 TTAAAGCATC AAGCAAAGTC TGAGCCATCA TTTTTTTAAA CGGTAGTACA TGTTTTGAAT
          1141 TAGCCTTAAA AAAGGTAAGG TAAGCTCAAC AGGGAAAAAA GTAG
          //
          Primer: phRLR2
          Sequence file: RDB05509_A7Ieb.seq check
          >05509_05509_A7Ie_phRLR2_D10_10_ABI24.ab1
              1 AACTTTCCTG TCTCGAAAGC CCAGTTTCTA TTGGTCTCCT TAAACCTGTC TTGTAACCTT
             61 GATACTTACC TGCCCAGTGC CTCACGACCA ACTTCTGCAG TACCCGGGTG AATTCTACGC
            121 GTCGACAGGC GACCCTGTCC CTCAAATCCT CTGGAGGGAC CGCGGTATCT TTCCAGGCAA
            181 GGGGACGCTG TGAGCGAGTG CTCGGAGGAG GTGCTATTAA CTCCGAGCAC TTAGCGAATG
            241 TGGCACCCCT GAAGTCGCCC CAGGTTGGGT CTCCCCCGGG GGCACCAGCC GGAAGCAGCC
            301 CTCGCCAGAG CCAGCGTTGG CAAGGAAGGA GGACTGGGCT CCTCCCCACC TGCCCCCCAC
            361 ACCGCCCTCC GGCCTCCCTG CTCCCAGCCG CGCTCCCCCG CCTGCCAGCA AAGGCGTGTT
            421 TGAGTGCGTT CACTCTGTTA AAAAGAAATC CGCCCCCGCC CCGTTTCCTT CCTCCGCGAT
            481 ACAACCTTCC TAACTGCCAA ATTGAATCGG GGTGTTTGGT GTCATAGGGA AAGTATGGCT
            541 TCTTCTTTTA ATCATAAGAA AAAGCAAAAC TATTCTTTCC TAGTTGTGAG AGCCCCACCG
            601 AGAATCGAAA TCACCTGTAC GACTAGAAAG TGTCCCCCTA CCCCCTCAAC CCTTGATTTT
            661 CAGGAGCGCG GGGTTCACTA AGTCAGAAAC CCTAGTTCAA AGGATTCCTT TTGGAGAGTC
            721 GGACTGCTCT CTCCTTCCCC TCCCCTTCCC CTCCTGCGTG TAAAACGGCT GTCTGGGGCA
            781 AGGGTTTCTC AGACGTGTAC ATTGCCTGGT ATAAGAGCAG ACTCTGAAAA GATGAGGTTT
            841 ATTTAATACG GACGGGGGAG AATTCTGCCT GTAGGCAGAT AGGAAAATGG GGAGGGAGTC
            901 ATTGGAAGGA CGGACTCCAT TCTCAAGTCA TAATTCCTAG ACCAGAAAAG TGCTCAGTGT
            961 TCTAGAAGCA GAGTTGCACA GTGATCAAGA CAGCTTCAAA TACTGTCTGT CTCGTCACAC
           1021 TCTCACATTT CTCTTCTTAC CTGAAATACT GCATTTTTCG TTATATGAGG GGATGATATG
          1081 GATGCCCTGC TTATAGGATG TTTGGAATAT GAGTAATATA CCTATAAGCC
          //
          Primer: pAxCALNL_F1
          Sequence file: RDB05509_A7Iec.seq check
          >05509_05509_A7Ie_pAxCALNL_F1_E10_13_ABI24.ab1
              1 CCATAATTGG AATCTTATCA TGTCTGCTCG AAGCGGCCGC TCTAGAATTA CTGCTCGTTC
             61 TTCAGCACGC GCTCCACGAA GCTCTTGATG TACTTACCCA TTTCATCTGG AGCGTCCTCC
            121 TGGCTGAAGT GGAGGCCCTT CACCTTCACG AACTCGGTGT TAGGGAACTT CTTAGCTCCC
            181 TCGACAATAG CGTTGGAAAA GAACCCAGGG TCGGACTCGA TGAACATCTT AGGCAGATCG
            241 TCGCTGGCCC GAAGGTAGGC GTTGTAGTTG CGGACAATCT GGACGACGTC GGGCTTGCCT
            301 CCCTTAACGA GAGGGATCTC GCGAGGCCAG GAGAGGGTAG GCCGTCTAAC CTCGCCCTTC
            361 TCCTTGAATG GCTCCAGGTA GGCAGCGAAC TCCTCAGGCT CCAGTTTCCG CATGATCTTG
            421 CTTGGGAGCA TGGTCTCGAC GAAGAAGTTA TTCTCAAGCA CCATTTTCTC GCCCTCTTCG
            481 CTCTTGATCA GGGCGATATC CTCCTCGATG TCAGGCCACT CGTCCCAGGA CTCGATCACG
            541 TCCACGACAC TCTCAGCATG GACGATGGCC TTGATCTTGT CTTGGTGCTC GTAGGAGTAG
            601 TGAAAGGCCA GACAAGCCCC CCAGTCGTGG CCCACAAAGA TGATTTTCTT TGGAAGGTTC
            661 AGCAGCTCGA ACCAAGCGGT GAGGTACTTG TAGTGATCCA GGAGGCGATA TGAGCCATTC
            721 CCGCTCTTGC CGGACTTACC CATTCCGATC AGATCAGGAT GATGCATCTA GCCACGGGCT
            781 CGATGTGAGG CACGACGTGC CTCCACAGGT AGCTGGAGGC AGCGTTACCA TGCAGAAAAA
            841 TCACGGCGTT CTCGGCGGTG CTTCTCGGAA TCATAGTAGT TGATGAAGGA GTCCAGCACG
            901 TTCATTTGCT TGCAGCGAGC CCACCACTGA GCCCAGTGAT CATGCGTTTG CGTGCTCGGG
            961 TCGTACACCT TGGAAGCCAT GTGGCTAGGC TATAGTGAGT CGGTATAGTA CTCTAGCTTA
           1021 GAGCTGTAAT GACCTGGGAG TGACTCTGTG AGAGAAGCCA AAGTGATGTC AGTAGACCAA
          1081 TAGTGCCTAT CAGAGCAGAG TCTTCCTTGT CGCAAGCAAG TCATGGCTTC TTACGC
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles


          2022.05.18

          GNP_filter3_RDBDEP_html_220516.pl