RDB05509 - pKM2L-phCDKN2

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pKM2L-phCDKN2 (#RDB05509)

Promoter Bank clone, Human p16 cyclin-dependent kinase inhibitor 2 (CDKN2/INK4A/CDK4I/MTS1) promoter

Alternative name	p1922-5F
Clone info.	PCR amplified human p16 cyclin-dependent kinase inhibitor 2 (CDKN2/INK4A/CDK4I/MTS1) promoter sequence was inserted into a Renilla luciferase reporter vector pKM2L (RDB04026). Cloned fragment: 21977927 to 21974960(NC_000009.12); relatively -2794 to +174, where +1 corresponds to 1 nt of NM_000077.4.
Comment	PCR cloning, forward primer, 1922 F2: 5' atctcccatcttcttcaccaatagc 3'; reverse primer, 1922 R2: 5' gaccctgtccctcaaatcctctg 3'; Entire sequence of promoter region has not been confirmed. Promoter activity has not been analyzed. Known difference (at least) : C to T substitution at 21,975,018nt of NC_000009.12.
Vector backbone	pKM2L (RDB04026) (Plasmid)
Size of vector backbone	4.2 kb
Selectable markers	Kan^r
Gene/insert name	Human p16 cyclin-dependent kinase inhibitor 2 (CDKN2/INK4A/CDK4I/MTS1) genomic DNA
Depositor\|Developer	DNA Bank, \|

Sequence	RDB05509z.seq

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Terms and conditions for distribution 1. The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. 2. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
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The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.

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Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link]
Terms and conditions for distribution	1. The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. 2. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. Additional terms and conditions: The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件	1. 本件リソースは学術研究機関にのみ提供し、学術研究にのみ利用することができる。　2. 謝辞の表明を必要とする。 MTAに書く付加的使用条件：本件リソースの使用は学術機関での学術研究に限る。本件リソースの使用にあたって、利用者はプロメガ社の限定使用条件に従う必要がある。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB05509	pKM2L-phCDKN2	DNA solution	JPY 9,460 (not-for-profit academic purpose) plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pKM2L-phCDKN2 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB05509).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB05509_A7Iep1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: M13_-40 Sequence file: RDB05509_A7Iea.seq
>05509_05509_A7Ie_M13_-40_E10_13_ABI24.ab1 1 CCGGCCGCGC CAGCTTGCAT GCCTGCAGGT CATTTAAATA GGGATAACAG GGTAATGATT 61 AAGATCTCGA GCTCTAAGCT TCACATATGC ATGCACTAGT GGCATCTCCC ATCTTCTTCA 121 CCAATAGCTA TATATATTTT TCACAAGAGC TGAAAGAGTT CTTGATGTAG GAATCCATGG 181 TAGAGTTTCA GAGAAATCCC TGAATTCACT GAAAGTTTTA TCTAGAAATA CATGTGCAAG 241 TGAACACATC TTTTTTAAAA AAAATCATTA CCTACTTTCT TTTTTGAGAA GAAGGTATTT 301 ATTTCAACAG ACTCTTGAAG GAGCCTACTC TTCCCACTCT CCCACCCCCA TTAAGAACCA 361 CTGTAGGCCG GGCACGATGG CTCATGCCTG TAATCCCAGC ACTTTGGGAG GCTAAGGTGG 421 GTGGATCACC TGAGGTCAGG AGTTCGAGAC AAGCCTAGCC AACATAGTGA AACCCCGTCT 481 CTACTAATAA TACAAAAATT AGCTGGGTAT GGCAGCATGT GCCTGTAATC CCAGCTACTC 541 GGGAGGCTGA GGCAGGAGAA TTGCTCGAAC CCGGGAGGCG GAGGTTGCAG TGAACCGAGA 601 GAGATCGTGC GGTGCCATTT CACTCCAGCC TGGGCAACAG AGCGAAACTC CATCTCAAAA 661 AAACACACAA AACAAACAAA CAAAAAGAAA GAACCATTGT ATTAGTGATG GAAATGTGTT 721 CCCTCCCTCC CATCCTGGCA ACCACTTTCT TCCTCCTCCA TCATAAAATA TCTTAAACTA 781 AACTAAAATA ATTTTATTTA TCGATAGTTT GAATTTTCCC TATCATTGCT ACACAGCTAA 841 TTGAGAGGTA CCCCGAGGAA AATATAAATG GTACAGTAAT GCATTGTAGA TTTTAATAAC 901 ATACTTGACA TCCCAAATTG TTTTCATTGG CTTCATTTTA AAAAACTACA TGTTTTAAAA 961 TCAAGCAGAC ACTAAAAGTA CAAGAATAAT ACTGGGTCTA CAAGGTTTTA AGTCAACCAG 1021 GGATTGAAAT ATAACTTTTA ACAGAGCTGG GATTATCCAG TAGCAGATTA AGCATGTGCT 1081 TTAAAGCATC AAGCAAAGTC TGAGCCATCA TTTTTTTAAA CGGTAGTACA TGTTTTGAAT 1141 TAGCCTTAAA AAAGGTAAGG TAAGCTCAAC AGGGAAAAAA GTAG //
Primer: phRLR2 Sequence file: RDB05509_A7Ieb.seq
>05509_05509_A7Ie_phRLR2_D10_10_ABI24.ab1 1 AACTTTCCTG TCTCGAAAGC CCAGTTTCTA TTGGTCTCCT TAAACCTGTC TTGTAACCTT 61 GATACTTACC TGCCCAGTGC CTCACGACCA ACTTCTGCAG TACCCGGGTG AATTCTACGC 121 GTCGACAGGC GACCCTGTCC CTCAAATCCT CTGGAGGGAC CGCGGTATCT TTCCAGGCAA 181 GGGGACGCTG TGAGCGAGTG CTCGGAGGAG GTGCTATTAA CTCCGAGCAC TTAGCGAATG 241 TGGCACCCCT GAAGTCGCCC CAGGTTGGGT CTCCCCCGGG GGCACCAGCC GGAAGCAGCC 301 CTCGCCAGAG CCAGCGTTGG CAAGGAAGGA GGACTGGGCT CCTCCCCACC TGCCCCCCAC 361 ACCGCCCTCC GGCCTCCCTG CTCCCAGCCG CGCTCCCCCG CCTGCCAGCA AAGGCGTGTT 421 TGAGTGCGTT CACTCTGTTA AAAAGAAATC CGCCCCCGCC CCGTTTCCTT CCTCCGCGAT 481 ACAACCTTCC TAACTGCCAA ATTGAATCGG GGTGTTTGGT GTCATAGGGA AAGTATGGCT 541 TCTTCTTTTA ATCATAAGAA AAAGCAAAAC TATTCTTTCC TAGTTGTGAG AGCCCCACCG 601 AGAATCGAAA TCACCTGTAC GACTAGAAAG TGTCCCCCTA CCCCCTCAAC CCTTGATTTT 661 CAGGAGCGCG GGGTTCACTA AGTCAGAAAC CCTAGTTCAA AGGATTCCTT TTGGAGAGTC 721 GGACTGCTCT CTCCTTCCCC TCCCCTTCCC CTCCTGCGTG TAAAACGGCT GTCTGGGGCA 781 AGGGTTTCTC AGACGTGTAC ATTGCCTGGT ATAAGAGCAG ACTCTGAAAA GATGAGGTTT 841 ATTTAATACG GACGGGGGAG AATTCTGCCT GTAGGCAGAT AGGAAAATGG GGAGGGAGTC 901 ATTGGAAGGA CGGACTCCAT TCTCAAGTCA TAATTCCTAG ACCAGAAAAG TGCTCAGTGT 961 TCTAGAAGCA GAGTTGCACA GTGATCAAGA CAGCTTCAAA TACTGTCTGT CTCGTCACAC 1021 TCTCACATTT CTCTTCTTAC CTGAAATACT GCATTTTTCG TTATATGAGG GGATGATATG 1081 GATGCCCTGC TTATAGGATG TTTGGAATAT GAGTAATATA CCTATAAGCC //
Primer: pAxCALNL_F1 Sequence file: RDB05509_A7Iec.seq
>05509_05509_A7Ie_pAxCALNL_F1_E10_13_ABI24.ab1 1 CCATAATTGG AATCTTATCA TGTCTGCTCG AAGCGGCCGC TCTAGAATTA CTGCTCGTTC 61 TTCAGCACGC GCTCCACGAA GCTCTTGATG TACTTACCCA TTTCATCTGG AGCGTCCTCC 121 TGGCTGAAGT GGAGGCCCTT CACCTTCACG AACTCGGTGT TAGGGAACTT CTTAGCTCCC 181 TCGACAATAG CGTTGGAAAA GAACCCAGGG TCGGACTCGA TGAACATCTT AGGCAGATCG 241 TCGCTGGCCC GAAGGTAGGC GTTGTAGTTG CGGACAATCT GGACGACGTC GGGCTTGCCT 301 CCCTTAACGA GAGGGATCTC GCGAGGCCAG GAGAGGGTAG GCCGTCTAAC CTCGCCCTTC 361 TCCTTGAATG GCTCCAGGTA GGCAGCGAAC TCCTCAGGCT CCAGTTTCCG CATGATCTTG 421 CTTGGGAGCA TGGTCTCGAC GAAGAAGTTA TTCTCAAGCA CCATTTTCTC GCCCTCTTCG 481 CTCTTGATCA GGGCGATATC CTCCTCGATG TCAGGCCACT CGTCCCAGGA CTCGATCACG 541 TCCACGACAC TCTCAGCATG GACGATGGCC TTGATCTTGT CTTGGTGCTC GTAGGAGTAG 601 TGAAAGGCCA GACAAGCCCC CCAGTCGTGG CCCACAAAGA TGATTTTCTT TGGAAGGTTC 661 AGCAGCTCGA ACCAAGCGGT GAGGTACTTG TAGTGATCCA GGAGGCGATA TGAGCCATTC 721 CCGCTCTTGC CGGACTTACC CATTCCGATC AGATCAGGAT GATGCATCTA GCCACGGGCT 781 CGATGTGAGG CACGACGTGC CTCCACAGGT AGCTGGAGGC AGCGTTACCA TGCAGAAAAA 841 TCACGGCGTT CTCGGCGGTG CTTCTCGGAA TCATAGTAGT TGATGAAGGA GTCCAGCACG 901 TTCATTTGCT TGCAGCGAGC CCACCACTGA GCCCAGTGAT CATGCGTTTG CGTGCTCGGG 961 TCGTACACCT TGGAAGCCAT GTGGCTAGGC TATAGTGAGT CGGTATAGTA CTCTAGCTTA 1021 GAGCTGTAAT GACCTGGGAG TGACTCTGTG AGAGAAGCCA AAGTGATGTC AGTAGACCAA 1081 TAGTGCCTAT CAGAGCAGAG TCTTCCTTGT CGCAAGCAAG TCATGGCTTC TTACGC //