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pKM2L-phPGHS2(2) (#RDB05504)

Promoter Bank clone, Human prostaglandin synthase-2/cyclooxygenase (PGHS-2/COX-2) promoter

Alternative name p1915-14F
Clone info. PCR amplified human prostaglandin synthase-2/cyclooxygenase (PGHS-2/COX-2) promoter sequence was inserted into a Renilla luciferase reporter vector pKM2L (RDB04026). Cloned fragment: 186,681,307 to 186,680,416 (NC_000001.11), relatively -880 to +12, where +1 corresponds to 1 nt of NM_000963.3.
Comment PCR cloning, forward primer, C6PB0043F: 5' cgcttcctttgtccatcagaaggca 3'; reverse primer, C6PB0043R: 5' tgacaattggtcgctaaccgagaga 3' Entire sequence of promoter region has not been confirmed.
Vector backbone pKM2L (RDB04026) (Plasmid)
Size of vector backbone 4.2 kb
Selectable markers Kan^r
Gene/insert name Human prostaglandin synthase-2/cyclooxygenase (PGHS-2/COX-2) genomic DNA
Depositor|Developer DNA Bank, |
 

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The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
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RDB05504 pKM2L-phPGHS2(2) DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pKM2L-phPGHS2(2) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB05504).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB05504_A9App1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: M13_-40 ()
Sequence file: RDB05504_A9Apa.seq check
>05504_05504_A9Ap_3_M13_-40_C01_03_ABI08.ab1
    1 GCGGGCCAAG GGCCCAGCTT GCATGCCTGC AGGTCATTTA AATAGGGATA ACAGGGTAAT
   61 GATTAAGATC TCGAGCTCTA AGCTTCACAT ATGCATGCAC TAGTGGCCGC TTCCTTTGTC
  121 CATCAGAAGG CAGGAAACTT TATATTGGTG ACCCGTGGAG CTCACATTAA CTATTTACAG
  181 GGTAACTGCT TAGGACCAGT ATTATGAGGA GAATTTACCT TTCCCGCCTC TCTTTCCAAG
  241 AAACAAGGAG GGGGTGAAGG TACGGAGAAC AGTATTTCTT CTGTTGAAAG CAACTTAGCT
  301 ACAAAGATAA ATTACAGCTA TGTACACTGA AGGTAGCTAT TTCATTCCAC AAAATAAGAG
  361 TTTTTTAAAA AGCTATGTAT GTATGTGCTG CATATAGAGC AGATATACAG CCTATTAAGC
  421 GTCGTCACTA AAACATAAAA CATGTCAGCC TTTCTTAACC TTACTCGCCC CAGTCTGTCC
  481 CGACGTGACT TCCTCGACCC TCTAAAGACG TACAGACCAG ACACGGCGGC GGCGGCGGGA
  541 GAGGGGATTC CCTGCGCCCC CGGACCTCAG GGCCGCTCAG ATTCCTGGAG AGGAAGCCAA
  601 GTGTCCTTCT GCCCTCCCCC GGTATCCCAT CCAAGGCGAT CAGTCCAGAA CTGGCTCTCG
  661 GAAGCGCTCG GGCAAAGACT GCGAAGAAGA AAAGACATCT GGCGGAAACC TGTGCGCCTG
  721 GGGCGGTGGA ACTCGGGGAG GAGAGGGAGG GATCAGACAG GAGAGTGGGG ACTACCCCCT
  781 CTGCTCCCAA AATTGGGGCA GCTTCCTGGG TTTCCGATTT TCTCATTTCC GTGGGTAAAA
  841 AACCCTGCCC CCACCGGGCT TACGCAATTT TTTTTAAGGG GAGAGGAGGG AAAATTTGTG
901 GGGGGTACGA AAAGGGCGGA AAAGAAACAG TCATTTCCGT CA
//
Primer: phRLR2 ()
Sequence file: RDB05504_A9Apb.seq check
>05504_05504_A9Ap_3_phRLR2_D01_04_ABI08.ab1
    1 GATACCCCCT TAGGGCCTTG ACAAGCCCAG GTTTTCTATT GGTCTCCTTA AAACCTTGTC
   61 TTGTAACCTT GATACTTACC TGCCCAGTGC CTCACGACCA ACTTCTGCAG TACCCGGGTG
  121 AATTCTACGC GTCGACAGGC TGACAATTGG TCGCTAACCG AGAGAACCTT CCTTTTTATA
  181 AGACTGAAAA CCAAGCCCAT GTGACGAAAT GACTGTTTCT TTCCGCCTTT TCGTACCCCC
  241 CACAAATTTT TCCCTCCTCT CCCCTTAAAA AAATTGCGTA AGCCCGGTGG GGGCAGGGTT
  301 TTTTACCCAC GGAAATGAGA AAATCGGAAA CCCAGGAAGC TGCCCCAATT TGGGAGCAGA
  361 GGGGGTAGTC CCCACTCTCC TGTCTGATCC CTCCCTCTCC TCCCCGAGTT CCACCGCCCC
  421 AGGCGCACAG GTTTCCGCCA GATGTCTTTT CTTCTTCGCA GTCTTTGCCC GAGCGCTTCC
  481 GAGAGCCAGT TCTGGACTGA TCGCCTTGGA TGGGATACCG GGGGAGGGCA GAAGGACACT
  541 TGGCTTCCTC TCCAGGAATC TGAGCGGCCC TGAGGTCCGG GGGCGCAGGG AATCCCCTCT
  601 CCCGCCGCCG CCGCCGTGTC TGGTCTGTAC GTCTTTAGAG GGTCGAGGAA GTCACGTCGG
  661 GACAGACTGG GGCGAGTAAG GTTAAGAAAG GCTGACATGT TTTATGTTTT AGTGACGACG
  721 CTTAATAGGC TGTATATCTG CTCTATATGC AGCACATACA TACATAGCTT TTTAAAAAAC
  781 TCTTATTTTG TGGAATGAAA TAGCTACCTT CAGTGTACAT AGCTGTAATT TATCTTTGTA
  841 GCTAAGTTGC TTTCAACAGA AGAAATACTG TTCTCCGTAC CTTCACCCCC TCCTTGTTTC
  901 TTGGAAAGAG AGGCGGGAAA AGGTAAATTC TCCTCATAAT ACTGGGTCCT AAGCAGTTAC
  961 CCTGTAAATA GTTAATGTGA GCTCCACGGG GTCACCAATA TAAAGGTTCC TGCCTTTCTG
 1021 ATGGACAAAG GAAGCGGCCA CTAGTGCATG CATATGTGAA GCTTAGAAGC TTCGAGATCT
 1081 TAATCATTAC CCTGTTATCC TATTTAAATG ACTTGCAAGG CATGCCAAGC TTGGCACTGG
 1141 CCGTCGTTTT ACACGGTCCT GACTTGGAAA AACCCTGGGC GTTACTCAAA CTTATCGCTT
 1201 GCAGCACATT CCCCGTTTCG CTCAGCTGGC TAATAGCAGA GGCTCGCACG ATCGCCACTT
1261 CCCAACATAG TTGTGCACCG
//
Primer: pAxCALNL_F1 ()
Sequence file: RDB05504_A9Apc.seq check
>05504_05504_A9Ap_3_pAxCALNL_F1_E01_05_ABI08.ab1
    1 CACTCAATGG TTTCTTTATC ATGGTCTGCT CGAAGCGGCC GCTCTAGAAT TACTGCTCGT
   61 TCTTCAGCAC GCGCTCCACG AAGCTCTTGA TGTACTTACC CATTTCATCT GGAGCGTCCT
  121 CCTGGCTGAA GTGGAGGCCC TTCACCTTCA CGAACTCGGT GTTAGGGAAC TTCTTAGCTC
  181 CCTCGACAAT AGCGTTGGAA AAGAACCCAG GGTCGGACTC GATGAACATC TTAGGCAGAT
  241 CGTCGCTGGC CCGAAGGTAG GCGTTGTAGT TGCGGACAAT CTGGACGACG TCGGGCTTGC
  301 CTCCCTTAAC GAGAGGGATC TCGCGAGGCC AGGAGAGGGT AGGCCGTCTA ACCTCGCCCT
  361 TCTCCTTGAA TGGCTCCAGG TAGGCAGCGA ACTCCTCAGG CTCCAGTTTC CGCATGATCT
  421 TGCTTGGGAG CATGGTCTCG ACGAAGAAGT TATTCTCAAG CACCATTTTC TCGCCCTCTT
  481 CGCTCTTGAT CAGGGCGATA TCCTCCTCGA TGTCAGGCCA CTCGTCCCAG GACTCGATCA
  541 CGTCCACGAC ACTCTCAGCA TGGACGATGG CCTTGATCTT GTCTTGGTGC TCGTAGGAGT
  601 AGTGAAAGGC CAGACAAGCC CCCCAGTCGT GGCCCACAAA GATGATTTTC TTTGGAAGGT
  661 TCAGCAGCTC GAACCAAGCG GTGAGGTACT TGTAGTGATC CAGGAGGCGA TATGAGCCAT
  721 TCCCGCTCTT GCCGGACTTA CCCATTCCGA TCAGATCAGG GATGATGCAT CTAGCCACGG
  781 GCTCGATGTG AGGCACGACG TGCCTCCACA GGTAGCTGGA GGCAGCGTTA CCATGCAGAA
  841 AAATCACGGC GTTCTCGGCG TGCTTCTCGG AATCATAGTA GTGATGAAAG GAGTCCAGCA
  901 CGTTCATTTG CTTGCAGCGA GCCCACCACT GAGGCCCAGT GATCATGCGT TGCGTTGCTC
  961 GGGGTCGTAC ACCCTTGGAA GCCATGGGTG GCTAGCCTAT AGTGAGTTCG TATTAAGTAC
 1021 TCTAGCCCTT AAGAGCTGTA ATTGAACTGG GAGTGGACAC CTGTGGAGAG AAAGGCAAAG
 1081 TGGATGTAAT TAGACCAATA GGTGCTATCA GAAAACGCAG AAGTCTTCTC TGTTCTCAAA
 1141 GGCCAGTTCA ATTGGATCTC TTTAAAACTG TTCTGTGTAC CTTTGATACC TTAACCTGTC
 1201 CATGTGCCTA CGAACAACCT TCTGACAGAA TCACCGGGTG GAATTTCAGA GCGTGTCAGA
1261 AACGAGCT
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles