RDB05501 - pKM2L-phPTGS1

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pKM2L-phPTGS1 (#RDB05501)

Promoter Bank clone, Human prostaglandin-endoperoxide synthase 1 (PTGS1) promoter

Alternative name	p1912-3F
Clone info.	PCR amplified human prostaglandin-endoperoxide synthase 1 (PTGS1) promoter sequence was inserted into a Renilla luciferase reporter vector pKM2L (RDB04026). Cloned fragment: 122370053 to 122371084(NC_000009.12); relatively -952 to +80, where +1 corresponds to 1 nt of NM_000962.3.
Comment	PCR cloning, forward primer, H5PB0040F: 5' ttggacctattccttcctccttcta 3'; reverse primer, H5PB0040R: 5' ggcgcggctgctggggtgcggg 3'; Entire sequence of promoter region has not been confirmed. Promoter activity has not been analyzed.
Vector backbone	pKM2L (RDB04026) (Plasmid)
Size of vector backbone	4.2 kb
Selectable markers	Kan^r
Gene/insert name	Human prostaglandin-endoperoxide synthase 1 genomic DNA
Depositor\|Developer	DNA Bank, \|

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The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.

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Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link]
Terms and conditions for distribution	1. The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. 2. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. Additional terms and conditions: The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.

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MTAに書く使用条件	1. 本件リソースは学術研究機関にのみ提供し、学術研究にのみ利用することができる。　2. 謝辞の表明を必要とする。 MTAに書く付加的使用条件：本件リソースの使用は学術機関での学術研究に限る。本件リソースの使用にあたって、利用者はプロメガ社の限定使用条件に従う必要がある。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB05501	pKM2L-phPTGS1	DNA solution	JPY 9,460 (not-for-profit academic purpose) plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pKM2L-phPTGS1 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB05501).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB05501_B0Ggp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pUC-M13_-100 (Pr0310) Region: insert 5' Sequence file: RDB05501_B0Gga.seq
>05501_05501_B0Gg_1_pUC-M13_-100_A09_03_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNGNNNNNN NNNNNNNNNN NCCTGCNNGN CATTTAAATA 61 GGGATAACAN GNNNNNNGAT NAAGATCTCG AGCTCTAAGC TTCACATATG CATGCACTAG 121 TGGCTTGTAC CTATTCCTTC CTCCTTCTAG GTGCTTCCTG AGCTGGCGAA AAGGAGAGCT 181 GGATGCTGGG ATTCTATAAA TTCGGCGGTG GATGTGAGTC TAGCTACAGC ATGGACCCTG 241 GCCAGCCCTG GAATCTGAGT TCAGAGATCT TTGAAAAAAT GCCTTCCGAT AACTGAGCAC 301 CTACTACATG CTGGACACTG CACCAGGAGA TTTGTGTGCA TTCCCTCATT GAATCTTACA 361 ACCACCCTCT GGGATGGTTT TTGCTATTAA GCCGATTTTA TAGATGAGAA TACTGAGGGC 421 TAGAAAAGAT AAGTACCTTG TCCAAGGTGA CACGGCCAGT AAGCTGTGGA GTCTGGATTT 481 GAACTAGGCT CTCTTGGANT TTGGAGCCCG GTCTTTTCAT TTCTGGCAGA GACCTGTGGC 541 TTCNGGGGGC GGAGCCCAGC CCATGGGGGA AGTAAGTCCT TCCTGTTAGA TCTGTCTGAN 601 GANTAGTGAG TTTCTCATCT AGGAAAACCA TCAGCAGGCC AGGCTGGACT GCNNCNTTTC 661 TAGCCCCCTT TCTTCTCTGC NGACTGCCCT GTTTCTANCC CCTTTTCTTC TCTACCCTTT 721 NCTGTNNAGT GCGTTAGANC CATTTTACNG AANNNGNNTC TGANACCCTT TCNTNTCTGC 781 NGGGGCAGGG TATGTGNNTN NNANGANCCG CTNNCATCCN NNNNNNTCTG NNCCNAANNC 841 CCNNNNGTGA NCNNCNNNCN NTNGANNNNC NNTCNCNNNN NNNNNN //
Primer: hRluc_N_rev (Pr0567) Region: hRluc, intron, insert 3' Sequence file: RDB05501_B0Ggb.seq
>05501_05501_B0Gg_1_hRluc_N_rev_B09_06_ABI24.ab1 1 NNNNNNNNNN NNNNTNNNNT TGNNGCGNNC CCNCNACTGN NGCCCAGTGN TCATGCGTTT 61 GCGTTGCTCG GGGTCGTACA CCTTGGAAGC CATGGTGGCT AGCCTATAGT GAGTCGTATT 121 AAGTACTCTA GCCTTAAGAG CTGTAATTGA ACTGGGAGTG GACACCTGTG GAGAGAAAGG 181 CAAAGTGGAT GTCAGTAAGA CCAATAGGTG CCTATCAGAA ACGCAAGAGT CTTCTCTGTC 241 TCGACAAGCC CAGTTTCTAT TGGTCTCCTT AAACCTGTCT TGTAACCTTG ATACTTACCT 301 GCCCAGTGCC TCACGACCAA CTTCTGCAGT ACCCGGGTGA ATTCTACGCG TCGACAGGCG 361 GCTCGGCTGC TGGGGTGCGG GACGCAGAGT GCAGGCTCCT GTGCGTGCGC CTCGCACACT 421 GCCCGGAGCT CCAGCCCATC CCCTCCCCAC CCTTCCCCCG GCTCCACCCC CGCTCCTCCC 481 TCCAGCTGTC ACCTCGGCTG CCCGCTTACT TCCTCTGCCC AGCCCCGGCC AAGGCATTTT 541 ATTAATTAGT GTATGCTCGC CGCTAAAGCG GCTTTTCACC CAAGATCCTT GAAGCACTTA 601 CGTTTCTGAT GCCTGGTGGC ACTAGATATA GACAGGAAAC TGANGCTCAG AGACGATGCT 661 CAATGCTGAN GTCACCCAGG GCTTANGGAC AGAACTGGGG ATGGAAGCGG CTCGTCTGAA 721 CCACATACCC TGCCCCGCAN ANAAGAAAGG GTCTCAGANT CNNCTTCTGT AAAATGNNTC 781 TAACGCACTC TACAGAAAAN GGTAGAGAAG AAAANGGGCT ANNAACAGGN CANNCTGCAN 841 ANAANAAAGG GGGNTANAAA NGGGGGCANN NNANCNNNNN NNNGCNNNNG NNTTCNNNNN 901 NNNNAAANTC ANTANNCCNN NNNNNNNN //
Primer: pAxCALNLF1 (Pr0166) Region: hRluc Sequence file: RDB05501_B0Ggc.seq
>05501_05501_B0Gg_1_pAxCALNLF1_C09_09_ABI24.ab1 1 NNNNNNNNNN NNNNNNGNNC TGCTNNNNNN GGCCGCTCTA GAATTACTGC TCGTTCTTCA 61 GCACGCGCTC CACGAAGCTC TTGATGTACT TACCCATTTC ATCTGGAGCG TCCTCCTGGC 121 TGAAGTGGAG GCCCTTCACC TTCACGAACT CGGTGTTAGG GAACTTCTTA GCTCCCTCGA 181 CAATAGCGTT GGAAAAGAAC CCAGGGTCGG ACTCGATGAA CATCTTAGGC AGATCGTCGC 241 TGGCCCGAAG GTAGGCGTTG TAGTTGCGGA CAATCTGGAC GACGTCGGGC TTGCCTCCCT 301 TAACGAGAGG GATCTCGCGA GGCCAGGAGA GGGTAGGCCG TCTAACCTCG CCCTTCTCCT 361 TGAATGGCTC CAGGTAGGCA GCGAACTCCT CAGGCTCCAG TTTCCGCATG ATCTTGCTTG 421 GGAGCATGGT CTCGACGAAG AAGTTATTCT CAAGCACCAT TTTCTCGCCC TCTTCGCTCT 481 TGATCAGGGC GATATCCTCC TCGATGTCAG GCCACTCGTC CCAGGACTCG ATCACGTCCA 541 CGACACTCTC AGCATGGACG ATGGCCTTGA TCTTGTCTTG GTGCTCGTAG GAGTAGTGAA 601 AGGCCAGACA AGCCCCCCAG TCGTGGCCCA CAAAGATGAT TTTCTTTGGA AGGTTCAGCA 661 GCTCGAACCA AGCGGTGAGG TACTTGTAGT GATCCAGGAN GCGATATGAG CCATTCCCGC 721 TCTTGCCGGA NTTACNCATT CCGATCAGAT CANGGNTGAT GCATCTAGCC ACGGGCTCNN 781 TGTGANGCAC GACNNGNNNC CACAGGNAGC TGGNNGCAGC GTTACCATGC ANAAAANTCA 841 CGGNNTTCNC GGCNNNNTNN NNNNNNTCAT ANNANTNNAT GNANNNANNC CNNNNNNNNN 901 ATTTNNCTNN NANNNNNNNN NN //