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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pKM2L-phTSHB

Promoter Bank clone, Human thyrotropin beta subunit(TSHbeta) promoter

Catalog number RDB05495
Resource name pKM2L-phTSHB
Alternative name p1904-2F
Clone info. PCR amplified human thyrotropin beta subunit(TSHbeta) promoter sequence was inserted into a Renilla luciferase reporter vector pKM2L (RDB04026). Cloned fragment: 115028634 to 115029861(NC_000001.11); relatively -1190 to +38, where +1 corresponds to 1 nt of NM_000549.4.
Comment PCR cloning, forward primer, H4PB0032F: 5' ggatcccttccctacaccatataga 3'; reverse primer, H4PB0032R: 5' ccttactttgcattggtgagcaga 3'; Entire sequence of promoter region has not been confirmed. Promoter activity has not been analyzed.
Vector backbone pKM2L (RDB04026) (Plasmid)
Size of vector backbone 4.2 kb
Selectable markers Kan^r
Gene/insert name Human thyroid stimulating hormone, beta genomic DNA
Depositor|Developer DNA Bank, |
Other clones in our bank
External Database
Human thyroid stimulating hormone, beta
          Reference sequence
            M23981, S70586

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR 1. The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. 2. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
          Additional terms and conditions for distribution The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
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          RDB05495 pKM2L-phTSHB DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB05495_A4Ca.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer:
          Sequence file: RDB05495_A4Caa.seq check
          >05495_05495_A4Ca_3_M13(-40)_C07_07.ab1
              1 CGCCAGCTCA GCTTGCATGC CTGCAGGTCA TTTAAATAGG GATAACAGGG TAATGATTAA
             61 GATCTCGAGC TCTAAGCTTC ACATATGCAT GCACTAGTGG CGGATCCCTT CCCTACACCA
            121 TATAGAAAAA TCAACTCAAC GTGGATTAAA GACTTACATG TAAAACCTAA AACTGTAAAA
            181 ACCCTCGAGG ATAACCTAGG AAATATCATT CCAGATGTAG GACCGGGCAG AGATTTCATG
            241 ACGAAGACGA CAAATGGAGT TTCAACAAGA ACAAAAGTTG ACAAATGGGA CCTTTAACTA
            301 AAGGGCTTCT GCATTATGGC CCAGAAACTT TCTCCAGGCA GTTTCTCCCT GTTTGTTTCC
            361 CATCTCTCTA GGATCGTGTA CTTTTGTTAC CTGATGTAAC AGGAAAAGAA ACTATCAACA
            421 TAATAAACAC ACAACCTACA GAATGGGAGA AAATATTTGC AAACTATGCA TCTGACAAAG
            481 GTCTAATATC CAGAATCTAT AAGGAGCTTA AACAATTTAT AAGCAAAAAC TGAAGAACCC
            541 CGTTAAAAAG TGGGCAAAAG ACATAAACAG ACACTTTTCA AAAGAAGATA TATACATGGC
            601 CAATAAGCAT ATGAAAAAAT GCTCACACCA CAAATCATTA GAGAAGTGAT ATTACCTTTT
            661 AATAGAAACT GAAGAGGTCA AGCATGCCAA TGTCATATGG CAAGTAAATG GCAGGGCCAA
            721 AATTTTATCT CAGGTCAGCT TGACATCCTG TCAGATGTGG CCGTGTTCCT CAGAGAGTCA
            781 TTCTTTCACA TGTGTAAATT TTACTTAATA TCTTAAATCC CCCAAAAATA TTTTGAACTA
            841 CACATTTTGT TCTAAACAAA ATTTTTAGAT GAGGAATAAT TTTATATATC TTTACATGAA
            901 CATTTAGGTA AAACTTGAAG CCCTCAAGCC CCAAATAAAT TTTTTATTCC CAAGACTTAT
            961 TTAGATGATG TTAGAGTGTT TTTTGAATTT GGTTTTTTCC CATTCTGTAT CTATCTTTTT
           1021 ATTAATATTA ATAAATAAAT ATTTAATAAA GAATTATATT TTAAAAAGGT AGTTTTTATT
           1081 TTTGCAACCA GAGAGAGCAA AAATGCCATG CCTTTTAAAT AACTTGTTCA AGGTTTCCCC
          1141 AAGGGG
          //
          Primer:
          Sequence file: RDB05495_A4Cab.seq check
          >05495_05495_A4Ca_3_phRLR2_F07_16.ab1
              1 AACTTCTTCC TTTCCCGACA GCCCAGTTTC TATTGGTCTC CTTAAACCTG TCTTGTAACC
             61 TTGATACTTA CCTGCCCAGT GCCTCACGAC CAACTTCTGC AGTACCCGGG TGAATTCTAC
            121 GCGTCGACAG GCCCTTACTT TGCATTGGTG AGCAGATGCT GTGGTGACCC AAACTAAAAG
            181 CTCTTGGTTC ATTTTATACC CTTCAGGATA ATCCCTCTGA TCTTCTTGTT TATACAATTG
            241 CATTTGATTT ACTGCTTTCT TATCTGAAAA GCATCTATTG AAAATTCATA CTGAATTCCA
            301 AATAAAATGT ATTTAGACAA ACAAGCTCAC AATATCTTTA CCTGGAAACT GACATTATTA
            361 AAGCATGCAT TTTCCTCTCT TCTGTTTCAA AATAAAATTA CCTTTTAAAT ATATTCTTTA
            421 TTAATATTTA TTTATTAATA TTAATAAAAG ATAGATACAG AATGGGAAAA ACCAAATTCA
            481 AAAAACACTC TAACATCATC TAAATAAGTC TTGGGAATAA AAATTTATTT GGGGCTTGAG
            541 GGCTTCAAGT TTTACCTAAA TGTTCATGTA AAGATATATA AAATTATTCC TCATCTAAAA
            601 ATTTTGTTTA GAACAAAATG TGTAGTTCAA AATATTTTTG GGGGATTTTA AGATATTAAG
            661 TAAAATTTAC ACATGTGAAA GAATGACTCT CTGAGGAACA CGGCCACATC TGACAGGATG
            721 TCAAGCTGAC CTGAGATAAA ATTTTGGCCC TGCCATTTAC TTGCCATATG ACATTGGCAT
            781 GCTTGACCTC TTCAGTTTCT ATTAAAAGGT AATATCACTT CTCTAATGAT TTGTGGTGTG
            841 AGCATTTTTT CATATGCTTA TTGGCCATGT ATATATCTTC TTTTGAAAAG TGTCTGTTTA
            901 TGTCTTTTGC CCACTTTTTT AACGGGGTTC TTCAGTTTTT TGCTTATAAA TTGTTTAAGC
            961 TCCTTATAGA TTCTGGGATA TTAGACCTTT TGTCAGATGC ATAGTTTGCA AATATTTTTC
           1021 TCCCATTCTG TAGTTTGTGT GTTATTATGT GATAGTTCTT TTTCCTGTTA CATCAGTAAC
           1081 AAAAGTAACA CGATTCTAGA AGAGATGGCA ACCAACAAGG CAGAAAACTT GGCCCTGGAG
          1141 A
          //

          Please visit Sequencing and PCR primers for primer information.

          References

          Original, user report and related articles

          2022.05.18

          GNP_filter3_RDBDEP_html_220516.pl