RDB05495 - pKM2L-phTSHB

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pKM2L-phTSHB (#RDB05495)

Promoter Bank clone, Human thyrotropin beta subunit(TSHbeta) promoter

Alternative name	p1904-2F
Clone info.	PCR amplified human thyrotropin beta subunit(TSHbeta) promoter sequence was inserted into a Renilla luciferase reporter vector pKM2L (RDB04026). Cloned fragment: 115028634 to 115029861(NC_000001.11); relatively -1190 to +38, where +1 corresponds to 1 nt of NM_000549.4.
Comment	PCR cloning, forward primer, H4PB0032F: 5' ggatcccttccctacaccatataga 3'; reverse primer, H4PB0032R: 5' ccttactttgcattggtgagcaga 3'; Entire sequence of promoter region has not been confirmed. Promoter activity has not been analyzed.
Vector backbone	pKM2L (RDB04026) (Plasmid)
Size of vector backbone	4.2 kb
Selectable markers	Kan^r
Gene/insert name	Human thyroid stimulating hormone, beta genomic DNA
Depositor\|Developer	DNA Bank, \|

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Terms and conditions for distribution 1. The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. 2. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
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The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.

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Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link]
Terms and conditions for distribution	1. The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. 2. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. Additional terms and conditions: The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件	1. 本件リソースは学術研究機関にのみ提供し、学術研究にのみ利用することができる。　2. 謝辞の表明を必要とする。 MTAに書く付加的使用条件：本件リソースの使用は学術機関での学術研究に限る。本件リソースの使用にあたって、利用者はプロメガ社の限定使用条件に従う必要がある。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB05495	pKM2L-phTSHB	DNA solution	JPY 9,460 (not-for-profit academic purpose) plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pKM2L-phTSHB was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB05495).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB05495_A4Ca.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: Sequence file: RDB05495_A4Caa.seq
>05495_05495_A4Ca_3_M13(-40)_C07_07.ab1 1 CGCCAGCTCA GCTTGCATGC CTGCAGGTCA TTTAAATAGG GATAACAGGG TAATGATTAA 61 GATCTCGAGC TCTAAGCTTC ACATATGCAT GCACTAGTGG CGGATCCCTT CCCTACACCA 121 TATAGAAAAA TCAACTCAAC GTGGATTAAA GACTTACATG TAAAACCTAA AACTGTAAAA 181 ACCCTCGAGG ATAACCTAGG AAATATCATT CCAGATGTAG GACCGGGCAG AGATTTCATG 241 ACGAAGACGA CAAATGGAGT TTCAACAAGA ACAAAAGTTG ACAAATGGGA CCTTTAACTA 301 AAGGGCTTCT GCATTATGGC CCAGAAACTT TCTCCAGGCA GTTTCTCCCT GTTTGTTTCC 361 CATCTCTCTA GGATCGTGTA CTTTTGTTAC CTGATGTAAC AGGAAAAGAA ACTATCAACA 421 TAATAAACAC ACAACCTACA GAATGGGAGA AAATATTTGC AAACTATGCA TCTGACAAAG 481 GTCTAATATC CAGAATCTAT AAGGAGCTTA AACAATTTAT AAGCAAAAAC TGAAGAACCC 541 CGTTAAAAAG TGGGCAAAAG ACATAAACAG ACACTTTTCA AAAGAAGATA TATACATGGC 601 CAATAAGCAT ATGAAAAAAT GCTCACACCA CAAATCATTA GAGAAGTGAT ATTACCTTTT 661 AATAGAAACT GAAGAGGTCA AGCATGCCAA TGTCATATGG CAAGTAAATG GCAGGGCCAA 721 AATTTTATCT CAGGTCAGCT TGACATCCTG TCAGATGTGG CCGTGTTCCT CAGAGAGTCA 781 TTCTTTCACA TGTGTAAATT TTACTTAATA TCTTAAATCC CCCAAAAATA TTTTGAACTA 841 CACATTTTGT TCTAAACAAA ATTTTTAGAT GAGGAATAAT TTTATATATC TTTACATGAA 901 CATTTAGGTA AAACTTGAAG CCCTCAAGCC CCAAATAAAT TTTTTATTCC CAAGACTTAT 961 TTAGATGATG TTAGAGTGTT TTTTGAATTT GGTTTTTTCC CATTCTGTAT CTATCTTTTT 1021 ATTAATATTA ATAAATAAAT ATTTAATAAA GAATTATATT TTAAAAAGGT AGTTTTTATT 1081 TTTGCAACCA GAGAGAGCAA AAATGCCATG CCTTTTAAAT AACTTGTTCA AGGTTTCCCC 1141 AAGGGG //
Primer: Sequence file: RDB05495_A4Cab.seq
>05495_05495_A4Ca_3_phRLR2_F07_16.ab1 1 AACTTCTTCC TTTCCCGACA GCCCAGTTTC TATTGGTCTC CTTAAACCTG TCTTGTAACC 61 TTGATACTTA CCTGCCCAGT GCCTCACGAC CAACTTCTGC AGTACCCGGG TGAATTCTAC 121 GCGTCGACAG GCCCTTACTT TGCATTGGTG AGCAGATGCT GTGGTGACCC AAACTAAAAG 181 CTCTTGGTTC ATTTTATACC CTTCAGGATA ATCCCTCTGA TCTTCTTGTT TATACAATTG 241 CATTTGATTT ACTGCTTTCT TATCTGAAAA GCATCTATTG AAAATTCATA CTGAATTCCA 301 AATAAAATGT ATTTAGACAA ACAAGCTCAC AATATCTTTA CCTGGAAACT GACATTATTA 361 AAGCATGCAT TTTCCTCTCT TCTGTTTCAA AATAAAATTA CCTTTTAAAT ATATTCTTTA 421 TTAATATTTA TTTATTAATA TTAATAAAAG ATAGATACAG AATGGGAAAA ACCAAATTCA 481 AAAAACACTC TAACATCATC TAAATAAGTC TTGGGAATAA AAATTTATTT GGGGCTTGAG 541 GGCTTCAAGT TTTACCTAAA TGTTCATGTA AAGATATATA AAATTATTCC TCATCTAAAA 601 ATTTTGTTTA GAACAAAATG TGTAGTTCAA AATATTTTTG GGGGATTTTA AGATATTAAG 661 TAAAATTTAC ACATGTGAAA GAATGACTCT CTGAGGAACA CGGCCACATC TGACAGGATG 721 TCAAGCTGAC CTGAGATAAA ATTTTGGCCC TGCCATTTAC TTGCCATATG ACATTGGCAT 781 GCTTGACCTC TTCAGTTTCT ATTAAAAGGT AATATCACTT CTCTAATGAT TTGTGGTGTG 841 AGCATTTTTT CATATGCTTA TTGGCCATGT ATATATCTTC TTTTGAAAAG TGTCTGTTTA 901 TGTCTTTTGC CCACTTTTTT AACGGGGTTC TTCAGTTTTT TGCTTATAAA TTGTTTAAGC 961 TCCTTATAGA TTCTGGGATA TTAGACCTTT TGTCAGATGC ATAGTTTGCA AATATTTTTC 1021 TCCCATTCTG TAGTTTGTGT GTTATTATGT GATAGTTCTT TTTCCTGTTA CATCAGTAAC 1081 AAAAGTAACA CGATTCTAGA AGAGATGGCA ACCAACAAGG CAGAAAACTT GGCCCTGGAG 1141 A //