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pKM2L-phPGHS2(1) (#RDB05480)

Promoter Bank clone, Human prostaglandin synthase-2/cyclooxygenase (PGHS-2/COX-2) promoter

Alternative name p1888-4F
Clone info. PCR amplified human prostaglandin synthase-2/cyclooxygenase (PGHS-2/COX-2) promoter sequence was inserted into a Renilla luciferase reporter vector pKM2L (RDB04026). Coned fragment: 186,682,006 to 186,680,342 (NC_000001.11), relatively -1579 to +86, where +1 corresponds to 1 nt of NM_000963.3.
Comment PCR cloning, forward primer, 1888 F3: 5' gaggaggagaaaggcttcctagatg 3'; reverse primer, 1888 R3: 5' gtctggctgtggagctgaaggag 3' Entire sequence of promoter region has not been confirmed.
Vector backbone pKM2L (RDB04026) (Plasmid)
Size of vector backbone 4.2 kb
Selectable markers Kan^r
Gene/insert name Human prostaglandin synthase-2/cyclooxygenase (PGHS-2/COX-2) genomic DNA
Depositor|Developer DNA Bank, |
 

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RDB05480 pKM2L-phPGHS2(1) DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pKM2L-phPGHS2(1) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB05480).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB05480_07Dkp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pBAC_lac_F (Pr0544)
Region: insert 5'
Sequence file: RDB05480_07Dka.seq check
>05480_05480_B1HbF_pBAC_lac_F_A01_01_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNCTN GCNTGCCTGC AGGTCATTTA AATAGGGANA ACAGGGTAAT
   61 GATTAAGATC TCNNGCTCTA AGCTTCACAT ATGCATGCAC TAGTGGCGAG GAGGAGAAAG
  121 GCTTCCTAGA TGAGATGGAA TTTTAGTCAT CCGTGTCTCA TGAAGAATCA GATGTGTACA
  181 CTAAGCAAAA CAGTTAAAAA AAAAACCTCC AAGTGAGTCT CTTATTTATT TTTTTCTTAT
  241 AAGACTTCTA CAAATTGAGG TACCTGGTGT AGTTTTATTT CAGGTTTTAT GCTGTCATTT
  301 TCCTGTAATG CTAAGGACTT AGGACATAAC TGAATTTTCT ATTTTCCACT TCTTTTCTGG
  361 TGTGTGTGTA TATATATATG TATATATACA CACACACATA TACATATATA TATTTTTTAG
  421 TATCTCACCC TCACATGCTC CTCCCTGAGC ACTACCCATG ATAGATGTTA AACAAAAGCA
  481 AAGATGAAAT TCCAACTGTC AAAATCTCCC TTCCATCTAA TTAATTCCTC ATCCAACTAT
  541 GTTCCAAAAC GAGAATAGAA AATTAGCCCC AATAAGCCCA GGCAACTGAA AAGTAAATGC
  601 TATGTTGTAC TTTGATCCAT GGTCACAACT CATAATCTTG GAAAAGTGGA CAGAANGACA
  661 AAAGAGTGAA CTTTAAAACT CGNNTTTATT TTACNNGTAT CTCCTATNNN NGGCTANNAN
721 CCAAANNAAN NNNNNNNNN
//
Primer: phRLR2primer (Pr0083)
Region: chimeric intron,insert 3'
Sequence file: RDB05480_07Dkb.seq check
>05480_05480_B1HbF_phRLR2primer_B01_02_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NTTTCNATTG GTCTCCTTAA ACCTGTCTTG TAACCTTGAT
   61 ACTTACCTGC CCAGTGCCTC ACGACCAACT TCTGCAGTAC CCGGGTGAAT TCTACGCGTC
  121 GACAGGCGTC TGGCTGTGGA GCTGAAGGAG GCGCTGCTGA GGAGTTCCTG GACGTGCTCC
  181 TGACGCTCAC TGCAAGTCGT ATGACAATTG GTCGCTAACC GAGAGAACCT TCCTTTTTAT
  241 AAGACTGAAA ACCAAGCCCA TGTGACGAAA TGACTGTTTC TTTCCGCCTT TTCGTACCCC
  301 CCACAAATTT TTCCCTCCTC TCCCCTTAAA AAAATTGCGT AAGCCCGGTG GGGGCAGGGT
  361 TTTTTACCCA CGGAAATGAG AAAATCGGAA ACCCAGGAAG CTGCCCCAAT TTGGGAGCAG
  421 AGGGGGTAGT CCCCACTCTC CTGTCTGATC CCTCCCTCTC CTCCCCGAGT TCCACCGCCC
  481 CAGGCGCACA GGTTTCCGCC AGATGTCTTT TCTTCTTCGC AGTCNNNNNN NGCGCTNNCN
541 ANAGNCAGNT CTNNNNTGNN CGNNNNNNNG NNNANNNGNN NNGNNANNNN NNNNNN
//
Primer: SV40pA_R (Pr0882)
Region: SV40 pA,hRluc 3'
Sequence file: RDB05480_07Dkc.seq check
>05480_05480_B1HbF_SV40pA_R_C01_03_ABI08.ab1
    1 NNNNNNNNNN NNNNNTTTNN NAAATAAAGC ATTTTTTTNN CTGCATTCTA GTTGTGGTTT
   61 GTCCAAACTC ATCAATGTAT CTTATCATGT CTGCTCGAAG CGGCCGCTCT AGAATTACTG
  121 CTCGTTCTTC AGCACGCGCT CCACGAAGCT CTTGATGTAC TTACCCATTT CATCTGGAGC
  181 GTCCTCCTGG CTGAAGTGGA GGCCCTTCAC CTTCACGAAC TCGGTGTTAG GGAACTTCTT
  241 AGCTCCCTCG ACAATAGCGT TGGAAAAGAA CCCAGGGTCG GACTCGATGA ACATCTTAGG
  301 CAGATCGTCG CTGGCCCGAA GGTAGGCGTT GTAGTTGCGG ACAATCTGGA CGACGTCGGG
  361 CTTGCCTCCC TTAACGAGAG GGATCTCGCG AGGCCAGGAG AGGGTAGGCC GTCTAACCTC
  421 GCCCTTCTCC TTGAATGGCT CCAGGTAGGC AGCGAACTCC TCAGGCTCCA GTTTCCGCAT
  481 GATCTTGCTT GGGAGCATGG TCTCGACGAA GAAGTTATTC TCAAGCACCA TTTTCTCGCC
  541 CTCTTCGCTC TTGATCAGGG CGATATCCTC CTCGATGTCA GGCCACTCGT CCCAGGACTC
  601 GATCACGTCC ACGACACTCT CAGCATGGAC GANGGNCTTG ANCTTGTCTT GNTGCTCGTA
  661 NGANTAGTGA AAGGNNAGAN NAGCCCCCCN NNCGNNNNNN NCAANGNNGN NTTTCTTNNN
721 NANNNNNNNN NN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

user_report Afroz, S., Dengue Virus Induced COX-2 Signaling Is Regulated Through Nutrient Sensor GCN2. Front. Immunol. 11: 1831 (2020). PMID 32903536. [link to RRC of NBRP]