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pKM2L-phBMP6 (#RDB05474)

Promoter Bank clone, Human bone morphogenetic protein 6 (BMP6) promoter

Alternative name p1882-5F
Clone info. The Human bone morphogenetic protein 6 (BMP6) promoter sequence was inserted into a Renilla luciferase reporter vector pKM2L (RDB04026). Cloned fragment: 7725636 to 7726955(NC_000006.12); relatively -463 to +857, where +1 corresponds to 1 nt of NM_001718.5; relatively -1142 to +178, where +1 corresponds to 1 nt of NM_001718.4.
Comment PCR cloning forward primer, B2PB0010F: 5' ctgccgtggaaagccacgtgtcc 3'; reverse primer, B2PB0010R: 5' ccccgcccggccgggctgccc 3' Reference: Fig.2 of Tamada, H., Biochim. Biophys. Acta 1395 (3): 247-251 (1998). Entire sequence of promoter region has not been confirmed.
Vector backbone pKM2L (RDB04026) (Plasmid)
Size of vector backbone 4.2 kb
Selectable markers Kan^r
Gene/insert name Human bone morphogenetic protein 6 genomic DNA
Depositor|Developer DNA Bank, |
 

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RDB05474 pKM2L-phBMP6 DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pKM2L-phBMP6 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB05474).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB14631_A7Bsp1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: M13_-40
Sequence file: RDB14631_A7Bsa.seq check
>05474_14631_A7Bs_M13(-40)_B08_02_ABI08.ab1
    1 CCGGCCGTCC AGCTTGCATG CCTGCAGGTC ATTTAATAGG GATAACAGGG TAATGATTAA
   61 GATCTCGAGC TCTAAGCTTC ACATATGCAT GCACTAGTGG CCTGCCGTGG AAAGCCACGT
  121 GTCCGCTAAA CGCCTACATT ACATCAATAA GAGAAACTAA GGAAGGGATG GAAAAGCTAT
  181 CATTTTTCTT TTCTTCCTCA GTTCTGGCAG TATAGTGATT TAATCGCTGT TATTATTTTT
  241 ATTTAGTTAA AGCATCAACT TAGGCCTTTT AAATGATGGT AAAAGAGAAA GCTTCAGATC
  301 GGGGTATTGG TCAGATCATT CTTACTAGCG ATGACCTCTT CGCCTTTGGC ACCTCGCGGC
  361 TCTTCGCATG CCTCCAAGAA TTCCCGGGTC TTGAAACCTA CGCGCGGCGG AGGCGACGCC
  421 TCTCGGGAGA GGGAGGGTGA AAGAAGGGGC GGGGCTATTT AAATAGCCCG CGAGCTTCGC
  481 GTCCCCATTG GCTGCCAGCC TGTCGGTTAC CGCGGGGCGG GGAGTCCCGG GGCTGGAGTG
  541 AGGTACCTGA GCCGGGCGCG CAGCAGAAAG CCCACGTTGG CCGCTGCGGG GAGCGCGGCG
  601 TGGAGAGGCG GGAGACCGAA TTCCGGCGCC GCCGCCGCGG CCGCTGCTCG GTGCACTAGC
  661 CCCCTTCCTT CCCATCCTTT CTGCGAGCGG GTTTGCTGGG CAGCCGGGCG ACCGCCGAAC
  721 GGAAAGCAAG ATCCTCGCCG CCTCCGCTTG AGGAGGCGTG CGGCGCGCGG AGATTTTGAG
  781 TGGGAGCGAC GGTGCCCGAG AGCTCAGGGG CGCGGAGTTC GTGAGCGAGA AGGAAGTTAA
  841 ACCTCGCGGA ATAGACTGGC ATTTCGGGAC GCCTTGTCGC CGCAGCCTGG GCCCTGCAAC
  901 GGGGTAAACT TCATGGTGGC CCTGCGATCT GGGGAGGGGC GTGCGCCACG GTGATGCCGG
  961 GCATCTCCCG CAGCTCGTGA GCGGCCCCGC TCCCCGCTGC CCCGGGTCCC ACTCAGTCGC
 1021 CAAGGGAGAG CGCGGGGCAG CGCACGTGTG CGGCCAGCGA GGCCCAGAGT GACCGCGCCG
 1081 CGACTCGCAG GAGCCAAGGG CGCAAGGCTG CAATGGCAGC CCGGACCTCA GACGGCACCT
 1141 GCCTGGAACC GCCGCGTCTT CTAGAGACCT GCGCGAGAGG CCTGTGTGAA GCTCCCTTCC
1201 TTCCCTCTCC AAGGCGGGAT CTCTCTTGGG GAGATCTC
//
Primer: phRLR2
Sequence file: RDB14631_A7Bsb.seq check
>05474_14631_A7Bs_phRLR2_E07_05_ABI08.ab1
    1 AACTTTTTCC TGTCTGACAA GCCCAGTTTC TATTGGTCTC CTTAAACCTG TCTTGTAACC
   61 TTGATACTTA CCTGCCCAGT GCCTCACGAC CAACTTCTGC AGTACCCGGG TGAATTCTAC
  121 GCGTCGACAG GCCCCCGCCC GGCCGGGCTG CCCCCGCGGA TCCCGCGAGG CGTGGAGCGG
  181 CGGAGCGAGG CCGGAGCGCG GCCGCTGTGG CCCTTGGCGT GAGCAGTCCC CGCCACCTCT
  241 CGGCGGGCTC GCTTCCCCTT CCTGGCCCTC AGTCCTTATC TCTCATGGTC GTCCGGGGCG
  301 CCCCCAGTTG CCCAGGCTAG AGAGGTGGCG AAGGGGCGAT CACCAGGAGA ACCGCTTGGA
  361 GGGGAGGAAG GGGAGCCTCA CAGCCTCGCG CAGGTCTCTA GAGGCGCGCG GTCCAGGCCA
  421 GGTGCGTCTG AGTCCGGGCT GCACTGCAGC CTGCGCCCTG GCTCCTGCGA GTCGCGGCGC
  481 GGTCACTCTG GGCCTCGCTG GCCGCACACG TGCGCTGCCC CGCGCTCTCC CTGGCGACTG
  541 AGTGGGACCC GGGGCAGCGG GGAGCGGGGC CGCTCACGAG CTGCGGGAGA TGCCCGGCAT
  601 CACCGTGGCG CACGCCCCTC CCCAGATCGC AGGGCCACCA TGAAGTTTAC CCCGTTGCAG
  661 GGCCCAGGCT GCGGCGACAA GGCGTCCCGA AATGCCAGTC TATTCCGCGA GGTTTAACTT
  721 CCTTCTCGCT CACGAACTCC GCGCCCCTGA GCTCTCGGGC ACCGTCGCTC CCACTCAAAA
  781 TCTCCGCGCG CCGCACGCCT CCTCAAGCGG AGGCGGCGAG GATCTTGCTT TCCGTTCGGC
  841 GGTCGCCCGG CTGCCCAGCA AACCCGCTCG CAGAAAGGAT GGGAAGGAAG GGGGCTAGTG
  901 CACCGAGCAG CGGCCGCGGC GGCGGCGCCG GAATTCGGTC TCCCGCCTCT CCACGCCGCG
  961 CTCCCCGCAG CGGCCAACGT GGGCTTTCTG CTGCGCGCCC GGCTCAGTAC CTCACTCCAG
 1021 CCCCGGGACT CCCCGGCCCG CGGTAACCGA CAGGCTGGCA GCCAATGGGG ACGCGAAGCT
 1081 CGCGGGCTAT TTAAATAGCC CCGCCCCTTC TTTCACCCTC CCTCTCCGAG GAGCGTCGCC
 1141 TCCGCCGCCG CGTAGTTTCA GGACCCGGAT TCTTGGAGCA TGCAGAGCCC CAGTGCAAGC
1201 GAAGTCATCG CTAGTAGAAT GATCTGGACC CATTACCCGA ATCTCTGGGA GCCTTTTC
//
Primer: pAxCALNL_F1
Sequence file: RDB14631_A7Bsc.seq check
>05474_14631_A7Bs_pAxCALNL_F1_F07_06_ABI08.ab1
    1 AATAAATGTA TCTTATCATG TCTGCTCGAA GCGGCCGCTC TAGAATTACT GCTCGTTCTT
   61 CAGCACGCGC TCCACGAAGC TCTTGATGTA CTTACCCATT TCATCTGGAG CGTCCTCCTG
  121 GCTGAAGTGG AGGCCCTTCA CCTTCACGAA CTCGGTGTTA GGGAACTTCT TAGCTCCCTC
  181 GACAATAGCG TTGGAAAAGA ACCCAGGGTC GGACTCGATG AACATCTTAG GCAGATCGTC
  241 GCTGGCCCGA AGGTAGGCGT TGTAGTTGCG GACAATCTGG ACGACGTCGG GCTTGCCTCC
  301 CTTAACGAGA GGGATCTCGC GAGGCCAGGA GAGGGTAGGC CGTCTAACCT CGCCCTTCTC
  361 CTTGAATGGC TCCAGGTAGG CAGCGAACTC CTCAGGCTCC AGTTTCCGCA TGATCTTGCT
  421 TGGGAGCATG GTCTCGACGA AGAAGTTATT CTCAAGCACC ATTTTCTCGC CCTCTTCGCT
  481 CTTGATCAGG GCGATATCCT CCTCGATGTC AGGCCACTCG TCCCAGGACT CGATCACGTC
  541 CACGACACTC TCAGCATGGA CGATGGCCTT GATCTTGTCT TGGTGCTCGT AGGAGTAGTG
  601 AAAGGCCAGA CAAGCCCCCC AGTCGTGGCC CACAAAGATG ATTTTCTTTG GAAGGTTCAG
  661 CAGCTCGAAC CAAGCGGTGA GGTACTTGTA GTGATCCAGG AGGCGATATG AGCCATTCCC
  721 GCTCTTGCCG GACTTACCCA TTCCGATCAG ATCAGGGATG ATGCATCTAG CCACGGGCTC
  781 GATGTGAGGC ACGACGTGCC TCCACAGGTA GCTGGAGGCA GCGTTACCAT GCAGAAAAAT
  841 CACGGCGTTC TCGGCGTGCT TCTCGGAATC ATAGTAGTTG ATGAAGGAGT CCAGCACGTT
  901 CATTTGCTTG CAGCGAGCCC ACCACTGAGC CCAGTGATCA TGCGTTTGCG TTGCTCGGGG
  961 TCGTACACCT TGGAAGCCAT GGTGGCTAGC CTATAGTGAG TCGTATTAAG TACTCTAGCC
 1021 TTAAGAGCTG TAATTGAACT GGGAGTGGAC ACCTGTGGAG AGAAGGCAAA GTGATGTCAG
 1081 TAGACCATAG GTGCCTATCA GAAACGCAGA GTCTTCTCTG TTCTCGACAA GCCCAGGTTT
 1141 CTATTGTCTT CTTAACTGTC TGTACCTTGA TACTACTGCC AAGTGCTCAC GACCACCTTC
1201 TGCAGTACTG TGATCTAACG CGTCGACAGG CCCCGCGCCC GGTCCGGGTG TC
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

reference Tamada, H., Molecular cloning and analysis of the 5'-flanking region of the human bone morphogenetic protein-6 (BMP-6). Biochim. Biophys. Acta 1395 (3): 247-251 (1998). PMID 9512655.
user_report Ohta, K., Role of hepcidin upregulation and proteolytic cleavage of ferroportin 1 in hepatitis C virus-induced iron accumulation. PLoS Pathog. 19 (8): e1011591 (2023). PMID 37585449. [link to RRC of NBRP]