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pKM2L-phBMP4P2 (#RDB05473)

Promoter Bank clone, Human bone morphogenetic protein 4 (BMP4), P2 promoter promoter

Alternative name p1881-5F
Clone info. PCR amplified human bone morphogenetic protein 4 (BMP4), P2 promoter sequence was inserted into a Renilla luciferase reporter vector pKM2L (RDB04026). Reference: Kawai, S., Bone 29 (1): 54-61 (2001), PMID 11472891; Shore, E.M., Calcif. Tissue Int. 63 (3): 221-229 (1998), PMID 9701626.
Comment The 2.2 kb genomic DNA corresponds to 3340 to 5582 nt of U43842.1 and 3339 to 5576 nt of AF210053.1, respectively. PCR cloning, forward primer, 1881F1: 5' atgggattcccgtccaagctatctc 3'; reverse primer, 1881R1: 5' cgagaaaacggtgaacagctttcgg 3'; Entire sequence of promoter region has not been confirmed. Promoter activity has not been analyzed.
Vector backbone pKM2L (RDB04026) (Plasmid)
Size of vector backbone 4.2 kb
Selectable markers Kan^r
Gene/insert name Human bone morphogenetic protein 4 (BMP4), P2 promoter genomic DNA
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB05473 pKM2L-phBMP4P2 DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pKM2L-phBMP4P2 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB05473).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB14630_A7Bsp1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: M13_-40
Sequence file: RDB14630_A7Bsa.seq check
>05473_14630_A7Bs_M13(-40)_A08_01_ABI08.ab1
    1 CAGGGCCAGT CCAGCTTGCA TGCCTGCAGG TCATTTAAAT AGGGATAACA GGGTAATGAT
   61 TAAGATCTCG AGCTCTAAGC TTCACATATG CATGCACTAG TGGCATGGGA TTCCCGTCCA
  121 AGCTATCTCG AGCCTGCAGC GCCACAGTCC CCGGCCCTCG CCCAGGTTCA CTGCAACCGT
  181 TCAGAGGTCC CCAGGAGCTG CTGCTGGCGA GCCCGCTACT GCAGGGACCT ATGGTGAGCA
  241 AGGCTACCTG GTGAGGGGAG ACAGGCAGAG GGGGTCTAGG AGCCTCCTTG GGGGGAAGAA
  301 GCTGGTCACA GGCTGTGACC GAGGCAAAAG GTGGCCTAAT TATTTTCCAA TAGTGGTGCT
  361 GGAGGTGGGG ATGCTGGCGC TGAAAGACCT TTAAATATCG GCTACTGCCC CTGCCCAGGC
  421 CTTCTCTGTC CAGCAGTCCC TGGGAGATTC TCACCTTTGG GAAGTGCGGG GCAGGAGAGC
  481 AGAAACAAGA GAAGCCCTTG GTAGGGGGGT CGTTGGGAAA AACTGTGGGG TCTTGGGCTG
  541 AACGCGTTGC CCACGGGCTG GAGGTTGCGA TCCCCGGACG GAAAGCGCGG GAGGAGGAAG
  601 GAGAGAACCG GCTCTGAGGT CCAGAGAGAG TGAGGGGGCA GAGCGACGGC GAGATGGGGA
  661 GAGAACACCT AGCTGGAGCA GGTTCTGCGG TAGAGAGCGC AGTCCTGCTG GCCTCTGGAG
  721 AGTGCGCGCC GCTACGGAGG CTGCGTCGAG GGGAGTGTCA CCCAATCTGG CCCCCAGCTG
  781 GCGGGGCGCC CTGAGAGCTT GCGAACTGCA GTTGCAGGAC GCGCCTTCTC CACGAGCTAT
  841 TTTCGTCGAC TTGCGGAACC CAAGGAACCT CGCCTCTATC ATTTCACGGT GTAGGGTCCC
  901 TAGAGACGAC AGCCAAGATC CCAGGGGCTC CCAGGACGCT TGTTCCTGCG GTGTCGTGTC
  961 CTATGGGGAG TTCCTGGCGG ACGAAAGGCG GACGCGCGGC TCTTCCTGGC CCTCCAGGCC
 1021 CGGAACCGAC GGGAAAGGTT CCCGTGATTC CCGAGTCCCT GCAGCTTCTT CAGCGGAGTT
 1081 GTCGGGGGCT AGAGCTCAGC ACTGCTTGAG ATGTTTCAGA TCGCGGTTTG TGAGTGAGCT
 1141 TGTGAGAACT AACCTCAAAG AATACATTAC CTGTAACTGG AGGCCTCAAC CGTGTTGAAC
//
Primer: phRLR2
Sequence file: RDB14630_A7Bsb.seq check
>05473_14630_A7Bs_phRLR2_B07_02_ABI08.ab1
    1 AATTTTCCCT TTTCTGGAAA GCCCAGTTTC TATTGGTCTC CTTAAACCTG TCTTGTAACC
   61 TTGATACTTA CCTGCCCAGT GCCTCACGAC CAACTTCTGC AGTACCCGGG TGAATTCTAC
  121 GCGTCGACAG GCCGAGAAAA CGGTGAACAG CTTTCGGCCT GCGCTCGACC TCTGCGTCTG
  181 CGTCTCTCTC GCCTCGGCTT CCCTTATTTT TTAAACCACC ACCACACTCC TTCCCCCGCC
  241 ACTTCCTTCC CCCACCCCCT TCCTCCGTTG CACCAGCAGC AGAGTCGCAC GCAGCAAATA
  301 CTCCTTCAAG AATTTTACCT ACCTACAGTT CAAGCAGTTA CTGGGATGTC CTGACTAATC
  361 GAAGATGCTG CCGCGCGCGT GGGTCGCTCT GCGCAAGGGC CTCTTCGAAA ACCCGACTAG
  421 GCGCAACTCA GCGTTCAGCA GGGCCGGGAG CGCCAGGTCG TCCCCGGGGC CCGGGCCCCA
  481 TGACTCCTGC CCCAAAGCCC ACTCCACCCG ACCTCCCTTT CCTGAGGCTG TTCCCAGTTG
  541 CTGCTTTGGG TCGCTCCGGA GCTCAAGAAC TCGGGTTGCC TGCCGCCCCA CTCTCCACGC
  601 ACATACTTGG TTTTCTTCTT AGGGGCATTG GCAGGTAGAC TTTGAGGAAG AAAAGTAAAG
  661 GATCGAACAG CTCAGCCCTC CCTCCCGACC GTGGATGCCC GGAGTCGACC AACACCTCAG
  721 GTCCGGGTGC GGAGGCCGCG GGCGCCCCTG CGCGACCGTC CGCGCCCGGC AAGAGCCGCG
  781 CGGCTTTCGC CTTTGCTGGT CCCGCGCCAC CGCTGGGGCG GGCTGCGAAA GGGTTGGGAA
  841 GAGCAAAGGG TTTTTTTGTT TTGTTTTGAG ACGCAGAAGC CCTTTAAAAA GCCCGCCGAG
  901 GAGAGGTCCA GAAGTAGAGA AAGCAGACGG AGGCAAGCTG TGCCCGCGGG GCAAAGGGAC
  961 AGTAGAAGGG CGGGCGCCCG GGTTCCCCGG AAACCCTCGG CCCCAAGAAT CTCCTGGGCG
1021 GGAGAGCGCG GTTCTAAACC GAGAGGATAG GAAGGGAGGG GGGAGTTGGT GTTTCAAT
//
Primer: pAxCALNL_F1
Sequence file: RDB14630_A7Bsc.seq check
>05473_14630_A7Bs_pAxCALNL_F1_C07_03_ABI08.ab1
    1 AACCAGGTTT TCTTATCATG TCTGCTCGAA GCGGCCGCTC TAGGAATTAC TGCTCGTTCT
   61 TCAGCACGCG CTCCACGAAG CTCTTGATGT ACTTACCCAT TTCATCTGGA GCGTCCTCCT
  121 GGCTGAAGTG GAGGCCCTTC ACCTTCACGA ACTCGGTGTT AGGGAACTTC TTAGCTCCCT
  181 CGACAATAGC GTTGGAAAAG AACCCAGGGT CGGACTCGAT GAACATCTTA GGCAGATCGT
  241 CGCTGGCCCG AAGGTAGGCG TTGTAGTTGC GGACAATCTG GACGACGTCG GGCTTGCCTC
  301 CCTTAACGAG AGGGATCTCG CGAGGCCAGG AGAGGGTAGG CCGTCTAACC TCGCCCTTCT
  361 CCTTGAATGG CTCCAGGTAG GCAGCGAACT CCTCAGGCTC CAGTTTCCGC ATGATCTTGC
  421 TTGGGAGCAT GGTCTCGACG AAGAAGTTAT TCTCAAGCAC CATTTTCTCG CCCTCTTCGC
  481 TCTTGATCAG GGCGATATCC TCCTCGATGT CAGGCCACTC GTCCCAGGAC TCGATCACGT
  541 CCACGACACT CTCAGCATGG ACGATGGCCT TGATCTTGTC TTGGTGCTCG TAGGAGTAGT
  601 GAAAGGCCAG ACAAGCCCCC CAGTCGTGGC CCACAAAGAT GATTTTCTTT GGAAGGTTCA
  661 GCAGCTCGAA CCAAGCGGTG AGGTACTTGT AGTGATCCAG GAGGCGATAT GAGCCATTCC
  721 CGCTCTTGCC GGACTTACCC ATTCCGATCA GATCAGGGAT GATGCATCTA GCCACGGGCT
  781 CGATGTGAGG CACGACGTGC CTCCACAGGT AGCTGGAGGC AGCGTTACCA TGCAGAAAAA
  841 TCACGGCGTT CTCGGCGTGC TTCTCGGAAT CATAGTAGTT GATGAAGGAG TCCAGCACGT
  901 TCATTTGCTT GCAGCGAGCC CACCACTGAG GCCCAGTGAT CATGCGTTTG CGTTGCTCGG
  961 GGTCGTACAC CTTGGAAGCC ATGGTGGCTA GCCTATAGTG AGTCGTATTA AGTACTCTAG
 1021 CCTTAAGAGC TGTAATTGAA CTGGAGTGGA CACTGTGGAG AGAAAGGCAA AGTGATGTCA
 1081 GTAGACCATA GGTGCCTATC AGAAACCGCA GAGTCTTCTC TGTCTCGACA GCCCAGGTTT
 1141 CTATTGGTCT CTAACCTGTC TGTAACCTTG ATACTAACCT GCAAGTGCCT ACGACACTCC
 1201 TGCCAGTACC TGGTGATCTA CCGTCGACGG CCGAGAAAAC GGGTGACACA GCCTTTCCGG
1261 C
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

reference Kawai, S., Characterization of human bone morphogenetic protein (BMP)-4 and -7 gene promoters: activation of BMP promoters by Gli, a sonic hedgehog mediator. Bone 29 (1): 54-61 (2001). PMID 11472891.
reference Shore, E.M., The human bone morphogenetic protein 4 (BMP-4) gene: molecular structure and transcriptional regulation. Calcif. Tissue Int. 63 (3): 221-229 (1998). PMID 9701626.