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pAxCALNLhCD22(forward) (#RDB05336)

Shuttle vector to generate rAd expressing human CD22

Alternative name 1633C11F
Clone info. Shuttle vector to generate rAd expressing human CD22. Cre-recombinase is required to remove stuffer sequence.
Comment Cre-recombinase is required to remove LNL stuffer sequence. Use COS-TPC method for the production of adenovirus.
Vector backbone pAxCALNLw (Cosmid, use packaging extracts for transforming E. coli host)
Selectable markers Amp^r
Gene/insert name Human CD22 cDNA
Depositor|Developer DNA Bank, |
 

Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature (Gene 200: 149-156, 1997) and acknowledgement to the DNA Bank are requested.
Additional terms and conditions:
Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.
Remarks Remember that you will be working with samples containing infectious virus.
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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 研究発表に際し、理研DNA Bankヘの謝辞ならびに次の論文の引用を必要とする (Gene 200: 149-156, 1997)。
MTAに書く付加的使用条件：
CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用し、大阪大学 宮崎純一博士への謝辞の表明を必要とする。
備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB05336 pAxCALNLhCD22(forward) DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pAxCALNLhCD22(forward) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB05336).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB05336_B1Isp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxCALNLF1 (Pr0166)
Region: lox P,insert 5'(5'UTR,CDS)
Sequence file: RDB05336_B1Isa.seq check
>05336_05336_B1Is_1_pAxCALNLF1_A02_02_ABI24.ab1
    1 NNNNNNNNTN ANNNNGTCTG GNNNNNTCCG AACGCGTATA ACTTCGTATA GCATACATTA
   61 TACGAAGTTA TCTCGAGTCG CTCGGTACGA TTTTCGAGCA CTGTTGGCCT ACTGGAGATG
  121 CTGCCAGGGT CCCTGAAGAG GGAAGACACG CGGAAACAGG CTTGCACCCA GACACGACAC
  181 CATGCATCTC CTCGGCCCCT GGCTCCTGCT CCTGGTTCTA GAATACTTGG CTTTCTCTGA
  241 CTCAAGTAAA TGGGTTTTTG AGCACCCTGA AACCCTCTAC GCCTGGGAGG GGGCCTGCGT
  301 CTGGATCCCC TGCACCTACA GAGCCCTAGA TGGTGACCTG GAAAGCTTCA TCCTGTTCCA
  361 CAATCCTGAG TATAACAAGA ACACCTCGAA GTTTGATGGG ACAAGACTCT ATGAAAGCAC
  421 AAAGGATGGG AAGGTTCCTT CTGAGCAGAA AAGGGTGCAA TTCCTGGGAG ACAAGAATAA
  481 GAACTGCACA CTGAGTATCC ACCCGGTGCA CCTCAATGAC AGTGGTCAGC TGGGGCTGAG
  541 GATGGAGTCC AAGACTGANA AATGGATGGA ACGAATACAC CTCAATGTCT CTGAAAGGCC
  601 TTTTCCACCT CATATCCNGC TCCCTCCAGA AATTCAAGAG TCCCAGGAAG TCACTCTGAN
  661 CTGCTNGCTG AATTTCTNCN GCTATGGGNN NCCNANCCNN NNGNANNGNN TCNNANNNGG
721 GGNNNNNNNN NN
//
Primer: pCAGGS_R1 (Pr0614)
Region: beta-globin polyA,insert 3'(polyA)
Sequence file: RDB05336_B1Isb.seq check
>05336_05336_B1Is_1_pCAGGS_R1_B02_05_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNCTA TTGNNNTGAA AATAAATTTC CTTTATTAGC CAGAAGTCAG
   61 ATGCTCAAGG GGCTTCATGA TGTCCCCATA ATTTTTGGCA GAGGGAAAAA GATCTCAGTG
  121 GTATTTGTGA GCCAGGGCAT TGGCCACACC AGCCACCACC TTCTGATAGG CAGCCTGCAC
  181 CTGAGGAGTG AATTGATTTT CGAGCACATG TGGCCTTTTT TTTTTTTTTT TTTTTTTTTT
241 TTTTTTTTTT TTTTTTTTTT TTTTTTTTTT NNNNNNNNNN
//
Primer: cd22-B_R (Pr1053)
Region: insert mid (3'UTR,CDS)
Sequence file: RDB05336_B1Isc.seq check
>05336_05336_B1Is_1_ccd22-B_R_F01_06_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNTC TNCGNNNTNN GTGTGTGTGT GTGTGCGTGC GTGTGTGTGT
   61 GTGTGTGCGC ACATGCGCGC AGGAGGAAGC CATGTGGCGG TGTCTGGGGA AACTCGGGGA
  121 CTTCCCTGGC CCCCGCTGCC CCCAGTGCCT CTGCTGCAGC CCATCCAGTG TCAATGTTTG
  181 AGGATCACAT AGTCCACATT TTCTTGTGCC TGAGGCCGCT CCCCGACCCC AAACTGGATC
  241 AGCTCTGAGT AATGAATCCC CTCATCTTCT GGAAAATCTG GAATGACGTT CTCATAGTCG
  301 CCCACTTGGC GCTTGTGCAA TGCTGAATAA GTGACCGTGT CATCGCAGTC CGGGGGAGGT
  361 CTCTGCATCT CTGAGGACTC TGCATCTCCA GTTCGTGGTA TGTTCATCTC GGGAAAGCGC
  421 AGGGTGGTGT AGCTAATGCC ATCTTCCATC ATTGGATTGT AGCATCCCAG GGAGTGGGGG
  481 CCTTCAGAGA GGGGGGCCCT TCTAACCTTT TTATTCCTCA CAAAGAAGCT CTGGCCGCTG
  541 GAATTCTCCT GAAGCCCCTG CTGGCTCTGT GTCCTCTTCC AACGTCGCTG GANCTTGAGC
  601 CCACAGATTG CCAGGATGAN GANGGNGAGG CANGANCCGA GTCCCACAGC CACTCNCCTG
  661 CCNATGGNCT CTGGGCTATA GTANACNGNG AGGGNGCTGA NANGNNAANN GNCCNTTNNC
721 NNNNNNNNNG NNCCCNNNNN NNNNN
//
Primer: Poly(A)region primer#0 (Pr1055)
Region: insert mid(3'UTR)
Sequence file: RDB05336_B1Isd.seq check
>05336_05336_B1Is_1_Poly(A)region primer#0_A01_01_ABI08.ab1
    1 NNNNNNNNNN NNNGNNNNCT CTGTCTCCCT GGCAGGTTAA TCCNCACCAG TGTGTCCATC
   61 TCAAANCCNN NNTCCNCNNA NTCAAGGGGC CGCTGATACG TACGTCTCCC CGTGCAATGC
  121 CTCACTTCCA AAGGAGAAGC CAGGCCAGGA CTGGGCCCCT TTCTACTTCG TTTCTGTCCT
  181 TTTCTGGGCT TTCCCTCAGA GGCAGAAACT GGGCATAATA TTCGTGAGTG GGGATGTCAG
  241 ATCAGGGGTA GAGGGCCAGA GAGATGGAGA AGGGAAGGAA AAATGATGAC GAGCAGATCC
  301 CAGGAGGGAC ACAAGCAGCT GGGGGTGGAG ATGGCCAGTG GCCGTGGTGG GGGCAGGGGG
  361 AGGTGTGCAT GTCAGGGCAG GTATTTAGAT GGATTTCTGA GTANCAAGGA GAGTGGAAGA
  421 GAACAGGGGC AGGAGTTTTG GAGGTTTGGG GTTACCCTCA CCAAAAAGAC TGGCTCTGAG
  481 CCAGGCACAA GGTTCTCCGC ANTGAGTGTG TGTGTGTGTG CGTGCGTGTG TGTGTGTGTG
  541 TGCGCACATG CGCGCANGAN GAAGCCATGT GGCGGTGTCT GGNGAAACTC GNGGACTTCC
  601 CTGGCCCCCG CTGCCCCCAG NGNNTCTGCT GNNNNCCATC NNNNGNNNNN GTTTGAGGNT
  661 CNCNTANTCC NNNTTTTTCN TGNGCCNGAG GCCNNTCNCC NACCCCNAAC TNGNATCANC
721 TNNNANNANT GNANNCCNNN NTCNTCTNNN NNNNNNN
//
Primer: phRLR1 (Pr0219)
Region: AmpR pro,Ad5 psi
Sequence file: RDB05336_B1Ise.seq check
>05336_05336_B1Is_1_phRLR1_G01_07_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NTNTTTGNNT GNNTTTAGAA AAATAAACAA ATAGGGGTTC
   61 CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGTCTAAGA AACCATTATT ATCATGACAT
  121 TAACCTATAA AAATAGGCGT ATCACGAGGC CCTTTCGTCT TCAAGAATTC CGGATCCGGG
  181 CCAATATGAT AATGAGGGGG TGGAGTTTGT GACGTGGCGC GGGGCGTGGG AACGGGGCGG
  241 GTGACGTAGT AGTGTGGCGG AAGTGTGATG TTGCAAGTGT GGCGGAACAC ATGTAAGCGA
  301 CGGATGTGGC AAAAGTGACG TTTTTGGTGT GCGCCGGTGT ACACAGGAAG TGACAATTTT
  361 CGCGCGGTTT TAGGCGGATG TTGTAGTAAA TTTGGGCGTA ACCGAGTAAG ATTTGGCCAT
  421 TTTCGCGGGA AAACTGAATA AGAGGAAGTG AAATCTGAAT AATTTTGTGT TACTCATAGC
  481 GCGTAATATT TGTCTAGGGC CGCGGGGACT TTGACCGTTT ACGTGGAGAC TCGCCCAGGT
  541 GTTTTTCTCA GGTGTTTTCC GCGTTCCGGG TCAAAGTTGG CGTTTTATTA TTATAGTCAG
  601 CATCGATTTA GCTNGNNCTG CNNNNCNAGG GNNCTCCATA AGAGANNAGG GACAGCTATG
  661 ACTGGGNNTA NTCNGGANNN GANNAAAANC TGGCNNGTAA ANNTGNNNNN AAANTTTNNN
721 NNNTNANNAN NNANNNNNNN NNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles