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pAxEFwtit2

Dual cassette vector to generate recombinant adenovirus containing EF1 alpha promoter

Catalog number RDB05215
Resource name pAxEFwtit2
Clone info. A dual cassette for constructing recombinant adenovirus containing EF1alpha promoter. Csp45I and PacI cab be used to generate recombinant adenovirus by transfection. Conversion to recombinant adenovirus was confirmed with HEK293 cell (Nov, 2005).
Comment The pAxEFwtit2 contains an expression cassette, consisting of the promoter of a gene for elongation factor 1alpha (EF-1alpha), a unique SwaI site and a polyadenylation signal from SV40 (S polyA), and was cloned into the SwaI site of pAxcwit2 (RDB 5212). The pAxEFwtit2 cosmid, digested with Csp45I or PacI, can generate rAd upon transfection of HEK293 cells and can also be used to generate rAd by the COS-TPC method [Miyake et al., Proc. Natl. Acad. Sci. USA 93, 1320-24, 1996].
Vector backbone Charomid 9-11 (Cosmid, use packaging extracts for transformation)
Size of vector backbone 11 kb
Selectable markers Amp^r
Gene/insert name AdV_5 - Genomic DNA
Depositor|Developer Saito, Izumu |
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External Database
AdV_5 -

            Reference sequence
              

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Terashima, M., Exp. Med., 21, 931-936(2003)).
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            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
            Remarks Remember that you will be working with samples containing infectious virus.
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            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Terashima, M., Exp. Med., 21, 931-936(2003))。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
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            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
            備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

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            RDB05215 pAxEFwtit2 DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Electronic file

            Remarks, protocol and/or map (pdf) RDB05215.pdf

            Original reference

            original Fukuda, H., Possible mechanism of adenovirus generation from a cloned viral genome tagged with nucleotides at its ends. Microbiol. Immunol., 50, 643-654 (2006). PMID 16924150.
            original Terashima, M., Exp. Med., 21, 931-936 (2003).

            Further references such as user reports and related articles (go to bottom)

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            Sequence information

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB05215_A3H9p1.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: EF1a-C_F
            Sequence file: RDB05215_A3H9a.seq check
            >05215_05215_A3H9_EF1a-C_F_D04_10.ab1
                1 GTACTCCAGC TCAGACAGTG GTTCAAGTTT TTTTCTTCCA TTTCAGGTGT CGTGAGGAAT
               61 TAATTCATCG ATTTAAATTA TAAACTAGTC TAGAATCGAT GAACTTGTTT ATTGCAGCTT
              121 ATAATGGTTA CAAATAAAGC AATAGCATCA CAAATTTCAC AAATAAAGCA TTTTTTTCAC
              181 TGCATTCTAG TTGTGGTTTG TCCAAACTCA TCAATGTATC TTATCATGTC TGGATCTCGA
              241 GGGCCCAAGC TGTTTAAACG ATGCTGACTA TAATAATAAA ACGCCAACTT TGACCCGGAA
              301 CGCGGAAAAC ACCTGAGAAA AACACCTGGG CGAGTCTCCA CGTAAACGGT CAAAGTCCCC
              361 GCGGCCCTAG ACAAATATTA CGCGCTATGA GTAACACAAA ATTATTCAGA TTTCACTTCC
              421 TCTTATTCAG TTTTCCCGCG AAAATGGCCA AATCTTACTC GGTTACGCCC AAATTTACTA
              481 CAACATCCGC CTAAAACCGC GCGAAAATTG TCACTTCCTG TGTACACCGG CGCACACCAA
              541 AAACGTCACT TTTGCCACAT CCGTCGCTTA CATGTGTTCC GCCACACTTG CAACATCACA
              601 CTTCCGCCAC ACTACTACGT CACCCGCCCC GTTCCCACGC CCCGCGCCAC GTCACAAACT
              661 CCACCCCCTC ATTATCATAT TGGCTTCAAT CCAAAATAAG GTATATTATT GATGATGTTC
              721 GAATTAATTA AATCGAAATC GAACATGCGG ATCGATCCGG AATTCTTGAA GACGAAAGGG
              781 CCTCGTGATA CGCCTATTTT TATAGGTTAA TGTCATGATA ATAATGGTTT CTTAGACGTC
              841 AGGTGGCACT TTTTCGGGGA AATGTGCGCG GAACCCCTAT TTGTTTATTT TTTCTAAATA
              901 CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTTC AATAATATTG
              961 AAAAAGGAAG AGTATGAGTA TTCAACATTT CCGTGTCGCC CTTTATTCCC TTTTTTTGCG
             1021 GCATTTTGCC CTTCCTGTTT TTTGCTCACC CAGAACGCTG GTTGAAAGGT AAAGATGGCT
             1081 GGAGGATCAG TTGGATGCAC GAAGTTGGGT AACATCGACT GGAATTCTTC AAACAAGGCG
            1141 GGTTACA
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Fukuda, H., Possible mechanism of adenovirus generation from a cloned viral genome tagged with nucleotides at its ends. Microbiol. Immunol., 50, 643-654 (2006). PMID 16924150.
            original Terashima, M., Exp. Med., 21, 931-936 (2003).
            user_report Araki, Y., Efficient recombinant production in mammalian cells using a novel IR/MAR gene amplification method. PLoS One, 7 (7): e41787 (2012). PMID 22844523.

            2022.05.18

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