Dual cassettevector to generate recombinant adenovirus
Clone info. | A dual cassette for constructing recombinant adenovirus. Improved version of pAxcwit. Csp45I and PacI can be used to generate recombinant adenovirus by transfection. Conversion to recombinant adenovirus was confirmed with HEK293 cell (Nov, 2005). |
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Comment | The pAxcwit2 cosmid is a derivative of the pAxcwit cosmid (RDB 3120) with additional PacI restriction sites and can also be used to generate rAd by the COS-TPC method [Miyake et al., Proc. Natl. Acad. Sci. USA 93, 1320-24, 1996]. The pAxcwit2 cosmid, digested with PacI or Csp45I, can generate rAd upon transfection of HEK293 cells. |
Vector backbone | charomid 9-11 (Cosmid, use packaging extracts for transforming E. coli host) |
Size of vector backbone | 11 kb |
Selectable markers | Amp^r |
Gene/insert name | AdV_5 - Genomic DNA |
Depositor|Developer | Saito, Izumu | |
Remarks, protocol and/or map (pdf) | RDB05212.pdf |
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Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms | Order form [Credit Card Payment ![]() ![]() ![]() MTA, for use for not-for-profit academic purpose [Word ![]() ![]() Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. |
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Terms and conditions for distribution | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Terashima, M., Exp. Med., 21, 931-936(2003)). |
Remarks | Remember that you will be working with samples containing infectious virus. |
必要書類 | 提供依頼書 ![]() ![]() 提供同意書 (MTA, 非営利学術目的用)[Word ![]() ![]() 手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 |
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MTAに書く使用条件 | 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Terashima, M., Exp. Med., 21, 931-936(2003))。 |
備考 | このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。 |
Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
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RDB05212 | pAxcwit2 | DNA solution |
Materials & Methods section:
The pAxcwit2 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB05212). |
Reference section:
Fukuda, H., Terashima, M., Koshikawa, M., Kanegae, Y., Saito, I., Possible mechanism of adenovirus generation from a cloned viral genome tagged with nucleotides at its ends. Microbiol. Immunol., 50, 643-654 (2006). PMID 16924150. [link to RRC of NBRP] |
Terashima, M., Kondo, S., Kanegae, Y., Saito, I., Exp. Med., 21, 931-936 (2003). |
Further references such as user reports and related articles (go to bottom)
RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.
Test sheet | RDB19995_B1Jfp1-1.pdf ![]() |
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Nucleotide sequence of a portion of this resource (if available).
Primer: phRLR1 (Pr0219) Region: Amp pro,ITR.1,Ad5 psi Sequence file: RDB19995_B1Jfa.seq ![]() |
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>05212_19995_B1Jf_3_phRLR1_G01.ab1 1 NNNNNNNNNN NNNNNNNNNN NTTTGNNTNN NTTTAGAAAA NTAAACAAAT AGGGGTTCCG 61 CGCACATTTC CCCGAAAAGT GCCACCTGAC GTCTAAGAAA CCATTATTAT CATGACATTA 121 ACCTATAAAA ATAGGCGTAT CACGAGGCCC TTTCGTCTTC AAGAATTCCG GATCGATCCG 181 CATGTTCGAT TTCGATTTAA TTAATTCGAA CATCATCAAT AATATACCTT ATTTTGGATT 241 GAAGCCAATA TGATAATGAG GGGGTGGAGT TTGTGACGTG GCGCGGGGCG TGGGAACGGG 301 GCGGGTGACG TAGTAGTGTG GCGGAAGTGT GATGTTGCAA GTGTGGCGGA ACACATGTAA 361 GCGACGGATG TGGCAAAAGT GACGTTTTTG GTGTGCGCCG GTGTACACAG GAAGTGACAA 421 TTTTCGCGCG GTTTTAGGCG GATGTTGTAG TAAATTTGGG CGTAACCGAG TAAGATTTGG 481 CCATTTTCGC GGGAAAACTG AATAAGAGGA AGTGAAATCT GAATAATTTT GTGTTACTCA 541 TAGCGCGTAA TATTTGTCTA GGGCCGCGGG GACTTTGACC GTTTACGTGG AGACTCGCCC 601 AGGTGTTTTT CTCAGGTGTT TTCCGCGTTC CGGGTCAAAG TTGGCGTTTT ATTATTATAG 661 TCAGCATCGA TTTAAATCGA TTGTCNNNNN NCGACGATGG ATCTGGAAGG TGNNNNNNTA 721 CNATGANACC CGCACCANGT GNNNNNNNCN GCNANTGTGG CGGNAAACNT ATTANNNNNN 781 NANCCTGTGA TGCTGGATGN NACNNANGNA NCTGANNNNN NNNNN // |
Primer: AxIT_R (PrPr347) Region: ITR.2 Sequence file: RDB19995_B1Jfb.seq ![]() |
>05212_19995_B1Jf_3_AxIT_R_D03_12_ABI24.ab1 1 NNNNNNNNNN NNNNNNTTNN NNNTTCCCNC GTTACGTCAC TTCCCATTTT AAGAAAACTA 61 CAATTCCCAA CACATACAAG TTACTCCGCC CTAAAACCTA CGTCACCCGC CCCGTTCCCA 121 CGCCCCGCGC CACGTCACAA ACTCCACCCC CTCATTATCA TATTGGCTTC AATCCAAAAT 181 AAGGTATATT ATTGATGATG TTCGAATTAA TTAAATCGAA ATCGAACATG CGGATCCTCT 241 AGAGTCAACA GCAGAAACAT ACAAGCTGTC AGCTTTGCAC AAGGGCCCAA CACCCTGCTC 301 ATCAAGAAGC ACTGTGGTTG CTGTGTTAGT AATGTGCAAA ACAGGAGGCA CATTTTCCCC 361 ACCTGTGTAG GTTCCAAAAT ATCTAGTGTT TTCATTTTTA CTTGGATCAG GAACCCAGCA 421 CTCCACTGGA TAAGCATTAT CCTTATCCAA AACAGCCTTG TGGTCAGTGT TCATCTGCTG 481 ACTGTCGACC TGCAGGCATG CAAGCTTTAA TGCGGTAGTT TATCACAGTT AAATTGCTAA 541 CGCAGTCAGG CANCGNGTAT TAAATCTAAC AATGCGCTCN TCGNNNTNCN TCGGCACCGT 601 NNCCNTGGAN GCTGTAGGCA TANGCTNGGN TATGCNNGNA CTGNCNGGNN CTCTNGNNGG 661 NNNTCNNNCA TTCNNNNNNN ANNNNNNGNN NNNATGNCGN GNNGCNNNNN NNNNN // |
Please visit Sequencing and PCR primers for primer information.
Original, user report and related articles
original | Fukuda, H., Possible mechanism of adenovirus generation from a cloned viral genome tagged with nucleotides at its ends. Microbiol. Immunol., 50, 643-654 (2006). PMID 16924150. [link to RRC of NBRP] |
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original | Terashima, M., Exp. Med., 21, 931-936 (2003). |
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