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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pAxcwit2

Dual cassettevector to generate recombinant adenovirus

Catalog number RDB05212
Resource name pAxcwit2
Clone info. A dual cassette for constructing recombinant adenovirus. Improved version of pAxcwit. Csp45I and PacI can be used to generate recombinant adenovirus by transfection. Conversion to recombinant adenovirus was confirmed with HEK293 cell (Nov, 2005).
Comment The pAxcwit2 cosmid is a derivative of the pAxcwit cosmid (RDB 3120) with additional PacI restriction sites and can also be used to generate rAd by the COS-TPC method [Miyake et al., Proc. Natl. Acad. Sci. USA 93, 1320-24, 1996]. The pAxcwit2 cosmid, digested with PacI or Csp45I, can generate rAd upon transfection of HEK293 cells.
Vector backbone charomid 9-11 (Cosmid, use packaging extracts for transformation)
Size of vector backbone 11 kb
Selectable markers Amp^r
Gene/insert name AdV_5 - Genomic DNA
Depositor|Developer Saito, Izumu |
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External Database
AdV_5 -

            Reference sequence
              

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Terashima, M., Exp. Med., 21, 931-936(2003)).
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            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
            Remarks Remember that you will be working with samples containing infectious virus.
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            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Terashima, M., Exp. Med., 21, 931-936(2003))。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
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            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
            備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

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            RDB05212 pAxcwit2 DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Electronic file

            Remarks, protocol and/or map (pdf) RDB05212.pdf

            Original reference

            original Fukuda, H., Possible mechanism of adenovirus generation from a cloned viral genome tagged with nucleotides at its ends. Microbiol. Immunol., 50, 643-654 (2006). PMID 16924150.
            original Terashima, M., Exp. Med., 21, 931-936 (2003).

            Further references such as user reports and related articles (go to bottom)

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            Sequence information

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB05212_A3Ahp1.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: pAxcwit2-Fw
            Sequence file: RDB05212_A3Aha.seq check
            >05212_05212_A3Ah_pAxcwit2-Fw_G08_20.ab1
                1 AATAGCGCGG GTGCAGGCCA GCACCAAGTG ATCGGGCCTC AGCTCCTCGG TCACATCCAG
               61 CATCACAGGC TGGTTCCTAA TATGTTTACC GCCACACTCG CAGGGTCTGC ACCTGGTGCG
              121 GGTCTCATCG TACCTCAGCA CCTTCCAGAT CCATCGTCGC GAGTCGACAA TCGATTTAAA
              181 TCGATGCTGA CTATAATAAT AAAACGCCAA CTTTGACCCG GAACGCGGAA AACACCTGAG
              241 AAAAACACCT GGGCGAGTCT CCACGTAAAC GGTCAAAGTC CCCGCGGCCC TAGACAAATA
              301 TTACGCGCTA TGAGTAACAC AAAATTATTC AGATTTCACT TCCTCTTATT CAGTTTTCCC
              361 GCGAAAATGG CCAAATCTTA CTCGGTTACG CCCAAATTTA CTACAACATC CGCCTAAAAC
              421 CGCGCGAAAA TTGTCACTTC CTGTGTACAC CGGCGCACAC CAAAAACGTC ACTTTTGCCA
              481 CATCCGTCGC TTACATGTGT TCCGCCACAC TTGCAACATC ACACTTCCGC CACACTACTA
              541 CGTCACCCGC CCCGTTCCCA CGCCCCGCGC CACGTCACAA ACTCCACCCC CTCATTATCA
              601 TATTGGCTTC AATCCAAAAT AAGGTATATT ATTGATGATG TTCGAATTAA TTAAATCGAA
              661 ATCGAACATG CGGATCGATC CGGAATTCTT GAAGACGAAA GGGCCTCGTG ATACGCCTAT
              721 TTTTATAGGT TAATGTCATG ATAATAATGG TTTCTTAGAC GTCAGGTGGC ACTTTTCGGG
              781 GAAATGTGCG CGGAACCCCT ATTTGTTTAT TTTTCTAAAT ACATTCAAAT ATGTATCCGC
              841 TCATGAGACA ATAACCCTGA TAAATGCTTC AATAATATTG AAAAAGGAAG AGTATGAGTA
              901 TTCAACATTT CCGTGTCGCC CTTATTCCCT TTTTTGCGGC ATTTTGCCTT CCTGTTTTTG
              961 CTCACCCAGA AACGCTGGTG AAAGTAAAAG ATGCTGAGAT CAGTTGGGGT GCACGAGTGG
             1021 GTTACATCGG AACTGGATCT CAACAGCGGT AGATCCTTTG AGAGTTTTTC GCCCCCGAGA
             1081 AACGTTTTCC ATGGATGGAG CCACTTTAAA GGTCTGCTAT GTGGGCGCGG GTTATTTATT
            1141 CCCGCTGTTT GAACGACCCG CGCCAAGGAG AGGCCAAAAT
            //
            Primer: pAxcwit2-Rv
            Sequence file: RDB05212_A3Ahb.seq check
            >05212_05212_A3Ah_pAxcwit2-Rv_H08_23.ab1
                1 CATAAGATGA GTGAATCTGA ATAATTTTGT GTTACTCATA GCGCGTAATA TTTGTCTAGG
               61 GCCGCGGGGA CTTTGACCGT TTACGTGGAG ACTCGCCCAG GTGTTTTTCT CAGGTGTTTT
              121 CCGCGTTCCG GGTCAAAGTT GGCGTTTTAT TATTATAGTC AGCATCGATT TAAATCGATT
              181 GTCGACTCGC GACGATGGAT CTGGAAGGTG CTGAGGTACG ATGAGACCCG CACCAGGTGC
              241 AGACCCTGCG AGTGTGGCGG TAAACATATT AGGAACCAGC CTGTGATGCT GGATGTGACC
              301 GAGGAGCTGA GGCCCGATCA CTTGGTGCTG GCCTGCACCC GCGCTGAGTT TGGCTCTAGC
              361 GATGAAGATA CAGATTGAGG TACTGAAATG TGTGGGCGTG GCTTAAGGGT GGGAAAGAAT
              421 ATATAAGGTG GGGGTCTTAT GTAGTTTTGT ATCTGTTTTG CAGCAGCCGC CGCCGCCATG
              481 AGCACCAACT CGTTTGATGG AAGCATTGTG AGCTCATATT TGACAACGCG CATGCCCCCA
              541 TGGGCCGGGG TGCGTCAGAA TGTGATGGGC TCCAGCATTG ATGGTCGCCC CGTCCTGCCC
              601 GCAAACTCTA CTACCTTGAC CTACGAGACC GTGTCTGGAA CGCCGTTGGA GACTGCAGCC
              661 TCCGCCGCCG CTTCAGCCGC TGCAGCCACC GCCCGCGGGA TTGTGACTGA CTTTGCTTTC
              721 CTGAGCCCGC TTGCAAGCAG TGCAGCTTCC CGTTCATCCG CCCGCGATGA CAAGTTGACG
              781 GCTCTTTTGG CACAATTGGA TTCTTTGACC CGGGAACTTA ATGTCGTTTC TCAGCAGCTG
              841 TTGGATCTGC GCCAGCAGTT TCTGCCCTGA AAGCTTCCTC CCCTCCCAAT GCGGTTTAAA
              901 ACATAAATAA AAAACCAGAC TCTGTTTGGA TTTGGATCAA GCAAGTGTCT TGCTGTCTTT
              961 ATTTAGGGTT TTGCGCGCGC GGTAGCCCGG GACCAGCGGT CTCGGTCGTT GAGGGTCCTG
             1021 TGTATTTTTT TTCCAGGACG TGTAAAGGTG ACCTCTGGGA TGTTCAGAAT ACCATGGGCC
             1081 ATAAGCCCGT CCTCTTGGGG GGTGGAAGGT AGGCACCCAC TGCAGAGCTT CCATGGCTGC
            1141 CGGGGTGGGG TGGTTTGGTT AGAAATGGAA TTTCCTCAG
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Fukuda, H., Possible mechanism of adenovirus generation from a cloned viral genome tagged with nucleotides at its ends. Microbiol. Immunol., 50, 643-654 (2006). PMID 16924150.
            original Terashima, M., Exp. Med., 21, 931-936 (2003).

            2022.05.18

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