Resource data sheet
Prev.
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pAxcwit2

Dual cassettevector to generate recombinant adenovirus

Catalog number RDB05212
Resource name pAxcwit2
Clone info. A dual cassette for constructing recombinant adenovirus. Improved version of pAxcwit. Csp45I and PacI can be used to generate recombinant adenovirus by transfection. Conversion to recombinant adenovirus was confirmed with HEK293 cell (Nov, 2005).
Comment The pAxcwit2 cosmid is a derivative of the pAxcwit cosmid (RDB 3120) with additional PacI restriction sites and can also be used to generate rAd by the COS-TPC method [Miyake et al., Proc. Natl. Acad. Sci. USA 93, 1320-24, 1996]. The pAxcwit2 cosmid, digested with PacI or Csp45I, can generate rAd upon transfection of HEK293 cells.
Vector backbone charomid 9-11 (Cosmid, use packaging extracts for transformation)
Size of vector backbone 11 kb
Selectable markers Amp^r
Gene/insert name AdV_5 - Genomic DNA
Depositor|Developer Saito, Izumu |
Other clones in our bank
External Database
AdV_5 -
            Reference sequence
              
             
            Remarks, protocol and/or map (pdf) RDB05212.pdf

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Terashima, M., Exp. Med., 21, 931-936(2003)).
            Ordering
            Order form [Credit Card Payment]  [Bank Transfer Payment]
            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
            Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
            Remarks Remember that you will be working with samples containing infectious virus.
            提供案内 (日本国内) [open/close]

            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Terashima, M., Exp. Med., 21, 931-936(2003))。
            提供依頼
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
            手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
            備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB05212 pAxcwit2 DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            How to cite this biological resource

            Materials & Methods section:

            The pAxcwit2 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB05212).

            Reference section:

            Fukuda, H., Terashima, M., Koshikawa, M., Kanegae, Y., Saito, I., Possible mechanism of adenovirus generation from a cloned viral genome tagged with nucleotides at its ends. Microbiol. Immunol., 50, 643-654 (2006). PMID 16924150. [link to RRC of NBRP]
            Terashima, M., Kondo, S., Kanegae, Y., Saito, I., Exp. Med., 21, 931-936 (2003).

            Further references such as user reports and related articles (go to bottom)

            References and tips

            Featured content

            QC test results

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB19995_B1Jfp1-1.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: phRLR1 (Pr0219)
            Region: Amp pro,ITR.1,Ad5 psi
            Sequence file: RDB19995_B1Jfa.seq check
            >05212_19995_B1Jf_3_phRLR1_G01.ab1
                1 NNNNNNNNNN NNNNNNNNNN NTTTGNNTNN NTTTAGAAAA NTAAACAAAT AGGGGTTCCG
               61 CGCACATTTC CCCGAAAAGT GCCACCTGAC GTCTAAGAAA CCATTATTAT CATGACATTA
              121 ACCTATAAAA ATAGGCGTAT CACGAGGCCC TTTCGTCTTC AAGAATTCCG GATCGATCCG
              181 CATGTTCGAT TTCGATTTAA TTAATTCGAA CATCATCAAT AATATACCTT ATTTTGGATT
              241 GAAGCCAATA TGATAATGAG GGGGTGGAGT TTGTGACGTG GCGCGGGGCG TGGGAACGGG
              301 GCGGGTGACG TAGTAGTGTG GCGGAAGTGT GATGTTGCAA GTGTGGCGGA ACACATGTAA
              361 GCGACGGATG TGGCAAAAGT GACGTTTTTG GTGTGCGCCG GTGTACACAG GAAGTGACAA
              421 TTTTCGCGCG GTTTTAGGCG GATGTTGTAG TAAATTTGGG CGTAACCGAG TAAGATTTGG
              481 CCATTTTCGC GGGAAAACTG AATAAGAGGA AGTGAAATCT GAATAATTTT GTGTTACTCA
              541 TAGCGCGTAA TATTTGTCTA GGGCCGCGGG GACTTTGACC GTTTACGTGG AGACTCGCCC
              601 AGGTGTTTTT CTCAGGTGTT TTCCGCGTTC CGGGTCAAAG TTGGCGTTTT ATTATTATAG
              661 TCAGCATCGA TTTAAATCGA TTGTCNNNNN NCGACGATGG ATCTGGAAGG TGNNNNNNTA
              721 CNATGANACC CGCACCANGT GNNNNNNNCN GCNANTGTGG CGGNAAACNT ATTANNNNNN
            781 NANCCTGTGA TGCTGGATGN NACNNANGNA NCTGANNNNN NNNNN
            //
            Primer: AxIT_R (PrPr347)
            Region: ITR.2
            Sequence file: RDB19995_B1Jfb.seq check
            >05212_19995_B1Jf_3_AxIT_R_D03_12_ABI24.ab1
                1 NNNNNNNNNN NNNNNNTTNN NNNTTCCCNC GTTACGTCAC TTCCCATTTT AAGAAAACTA
               61 CAATTCCCAA CACATACAAG TTACTCCGCC CTAAAACCTA CGTCACCCGC CCCGTTCCCA
              121 CGCCCCGCGC CACGTCACAA ACTCCACCCC CTCATTATCA TATTGGCTTC AATCCAAAAT
              181 AAGGTATATT ATTGATGATG TTCGAATTAA TTAAATCGAA ATCGAACATG CGGATCCTCT
              241 AGAGTCAACA GCAGAAACAT ACAAGCTGTC AGCTTTGCAC AAGGGCCCAA CACCCTGCTC
              301 ATCAAGAAGC ACTGTGGTTG CTGTGTTAGT AATGTGCAAA ACAGGAGGCA CATTTTCCCC
              361 ACCTGTGTAG GTTCCAAAAT ATCTAGTGTT TTCATTTTTA CTTGGATCAG GAACCCAGCA
              421 CTCCACTGGA TAAGCATTAT CCTTATCCAA AACAGCCTTG TGGTCAGTGT TCATCTGCTG
              481 ACTGTCGACC TGCAGGCATG CAAGCTTTAA TGCGGTAGTT TATCACAGTT AAATTGCTAA
              541 CGCAGTCAGG CANCGNGTAT TAAATCTAAC AATGCGCTCN TCGNNNTNCN TCGGCACCGT
              601 NNCCNTGGAN GCTGTAGGCA TANGCTNGGN TATGCNNGNA CTGNCNGGNN CTCTNGNNGG
            661 NNNTCNNNCA TTCNNNNNNN ANNNNNNGNN NNNATGNCGN GNNGCNNNNN NNNNN
            //

            Please visit Sequencing and PCR primers for primer information.

            References

            Original, user report and related articles

            original Fukuda, H., Possible mechanism of adenovirus generation from a cloned viral genome tagged with nucleotides at its ends. Microbiol. Immunol., 50, 643-654 (2006). PMID 16924150. [link to RRC of NBRP]
            original Terashima, M., Exp. Med., 21, 931-936 (2003).

            2023.06.15

            GNP_filter3_RDBDEP_html_230615.pl