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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pAxcwit2 (#RDB05212)

Dual cassettevector to generate recombinant adenovirus

Clone info. A dual cassette for constructing recombinant adenovirus. Improved version of pAxcwit. Csp45I and PacI can be used to generate recombinant adenovirus by transfection. Conversion to recombinant adenovirus was confirmed with HEK293 cell (Nov, 2005).
Comment The pAxcwit2 cosmid is a derivative of the pAxcwit cosmid (RDB 3120) with additional PacI restriction sites and can also be used to generate rAd by the COS-TPC method [Miyake et al., Proc. Natl. Acad. Sci. USA 93, 1320-24, 1996]. The pAxcwit2 cosmid, digested with PacI or Csp45I, can generate rAd upon transfection of HEK293 cells.
Vector backbone charomid 9-11 (Cosmid, use packaging extracts for transforming E. coli host)
Size of vector backbone 11 kb
Selectable markers Amp^r
Gene/insert name AdV_5 - Genomic DNA
Depositor|Developer Saito, Izumu |
 
Remarks, protocol and/or map (pdf) RDB05212.pdf

Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Terashima, M., Exp. Med., 21, 931-936(2003)).
Remarks Remember that you will be working with samples containing infectious virus.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Terashima, M., Exp. Med., 21, 931-936(2003))。
備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB05212 pAxcwit2 DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pAxcwit2 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB05212).

Reference section:

Fukuda, H., Terashima, M., Koshikawa, M., Kanegae, Y., Saito, I., Possible mechanism of adenovirus generation from a cloned viral genome tagged with nucleotides at its ends. Microbiol. Immunol., 50, 643-654 (2006). PMID 16924150. [link to RRC of NBRP]
Terashima, M., Kondo, S., Kanegae, Y., Saito, I., Exp. Med., 21, 931-936 (2003).

Further references such as user reports and related articles (go to bottom)


QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB19995_B1Jfp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: phRLR1 (Pr0219)
Region: Amp pro,ITR.1,Ad5 psi
Sequence file: RDB19995_B1Jfa.seq check
>05212_19995_B1Jf_3_phRLR1_G01.ab1
    1 NNNNNNNNNN NNNNNNNNNN NTTTGNNTNN NTTTAGAAAA NTAAACAAAT AGGGGTTCCG
   61 CGCACATTTC CCCGAAAAGT GCCACCTGAC GTCTAAGAAA CCATTATTAT CATGACATTA
  121 ACCTATAAAA ATAGGCGTAT CACGAGGCCC TTTCGTCTTC AAGAATTCCG GATCGATCCG
  181 CATGTTCGAT TTCGATTTAA TTAATTCGAA CATCATCAAT AATATACCTT ATTTTGGATT
  241 GAAGCCAATA TGATAATGAG GGGGTGGAGT TTGTGACGTG GCGCGGGGCG TGGGAACGGG
  301 GCGGGTGACG TAGTAGTGTG GCGGAAGTGT GATGTTGCAA GTGTGGCGGA ACACATGTAA
  361 GCGACGGATG TGGCAAAAGT GACGTTTTTG GTGTGCGCCG GTGTACACAG GAAGTGACAA
  421 TTTTCGCGCG GTTTTAGGCG GATGTTGTAG TAAATTTGGG CGTAACCGAG TAAGATTTGG
  481 CCATTTTCGC GGGAAAACTG AATAAGAGGA AGTGAAATCT GAATAATTTT GTGTTACTCA
  541 TAGCGCGTAA TATTTGTCTA GGGCCGCGGG GACTTTGACC GTTTACGTGG AGACTCGCCC
  601 AGGTGTTTTT CTCAGGTGTT TTCCGCGTTC CGGGTCAAAG TTGGCGTTTT ATTATTATAG
  661 TCAGCATCGA TTTAAATCGA TTGTCNNNNN NCGACGATGG ATCTGGAAGG TGNNNNNNTA
  721 CNATGANACC CGCACCANGT GNNNNNNNCN GCNANTGTGG CGGNAAACNT ATTANNNNNN
781 NANCCTGTGA TGCTGGATGN NACNNANGNA NCTGANNNNN NNNNN
//
Primer: AxIT_R (PrPr347)
Region: ITR.2
Sequence file: RDB19995_B1Jfb.seq check
>05212_19995_B1Jf_3_AxIT_R_D03_12_ABI24.ab1
    1 NNNNNNNNNN NNNNNNTTNN NNNTTCCCNC GTTACGTCAC TTCCCATTTT AAGAAAACTA
   61 CAATTCCCAA CACATACAAG TTACTCCGCC CTAAAACCTA CGTCACCCGC CCCGTTCCCA
  121 CGCCCCGCGC CACGTCACAA ACTCCACCCC CTCATTATCA TATTGGCTTC AATCCAAAAT
  181 AAGGTATATT ATTGATGATG TTCGAATTAA TTAAATCGAA ATCGAACATG CGGATCCTCT
  241 AGAGTCAACA GCAGAAACAT ACAAGCTGTC AGCTTTGCAC AAGGGCCCAA CACCCTGCTC
  301 ATCAAGAAGC ACTGTGGTTG CTGTGTTAGT AATGTGCAAA ACAGGAGGCA CATTTTCCCC
  361 ACCTGTGTAG GTTCCAAAAT ATCTAGTGTT TTCATTTTTA CTTGGATCAG GAACCCAGCA
  421 CTCCACTGGA TAAGCATTAT CCTTATCCAA AACAGCCTTG TGGTCAGTGT TCATCTGCTG
  481 ACTGTCGACC TGCAGGCATG CAAGCTTTAA TGCGGTAGTT TATCACAGTT AAATTGCTAA
  541 CGCAGTCAGG CANCGNGTAT TAAATCTAAC AATGCGCTCN TCGNNNTNCN TCGGCACCGT
  601 NNCCNTGGAN GCTGTAGGCA TANGCTNGGN TATGCNNGNA CTGNCNGGNN CTCTNGNNGG
661 NNNTCNNNCA TTCNNNNNNN ANNNNNNGNN NNNATGNCGN GNNGCNNNNN NNNNN
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Fukuda, H., Possible mechanism of adenovirus generation from a cloned viral genome tagged with nucleotides at its ends. Microbiol. Immunol., 50, 643-654 (2006). PMID 16924150. [link to RRC of NBRP]
original Terashima, M., Exp. Med., 21, 931-936 (2003).