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pAxcwit2

Dual cassettevector to generate recombinant adenovirus

Catalog number RDB05212
Resource name pAxcwit2
Clone info. A dual cassette for constructing recombinant adenovirus. Improved version of pAxcwit. Csp45I and PacI can be used to generate recombinant adenovirus by transfection. Conversion to recombinant adenovirus was confirmed with HEK293 cell (Nov, 2005).
The pAxcwit2 cosmid is a derivative of the pAxcwit cosmid (RDB 3120) with additional PacI restriction sites and can also be used to generate rAd by the COS-TPC method [Miyake et al., Proc. Natl. Acad. Sci. USA 93, 1320-24, 1996]. The pAxcwit2 cosmid, digested with PacI or Csp45I, can generate rAd upon transfection of HEK293 cells.
Vector backbone charomid 9-11 (Cosmid, use packaging extracts for transformation)
Size of vector backbone 11 kb
Selectable markers Amp^r
Gene/insert name AdV_5 - Genomic DNA
Depositor Saito, Izumu |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Terashima, M., Exp. Med., 21, 931-936(2003)).
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB05212 pAxcwit2 DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file PDF RDB05212.pdf from Depositor

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References

original Fukuda, H., Possible mechanism of adenovirus generation from a cloned viral genome tagged with nucleotides at its ends. Microbiol. Immunol., 50, 643-654 (2006). PMID 16924150.
original Terashima, M., Exp. Med., 21, 931-936 (2003).

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB05212_A3Ahp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxcwit2-Fw RDB05212_A3Aha.seq
>05212_05212_A3Ah_pAxcwit2-Fw_G08_20.ab1
    1 AATAGCGCGG GTGCAGGCCA GCACCAAGTG ATCGGGCCTC AGCTCCTCGG TCACATCCAG
   61 CATCACAGGC TGGTTCCTAA TATGTTTACC GCCACACTCG CAGGGTCTGC ACCTGGTGCG
  121 GGTCTCATCG TACCTCAGCA CCTTCCAGAT CCATCGTCGC GAGTCGACAA TCGATTTAAA
  181 TCGATGCTGA CTATAATAAT AAAACGCCAA CTTTGACCCG GAACGCGGAA AACACCTGAG
  241 AAAAACACCT GGGCGAGTCT CCACGTAAAC GGTCAAAGTC CCCGCGGCCC TAGACAAATA
  301 TTACGCGCTA TGAGTAACAC AAAATTATTC AGATTTCACT TCCTCTTATT CAGTTTTCCC
  361 GCGAAAATGG CCAAATCTTA CTCGGTTACG CCCAAATTTA CTACAACATC CGCCTAAAAC
  421 CGCGCGAAAA TTGTCACTTC CTGTGTACAC CGGCGCACAC CAAAAACGTC ACTTTTGCCA
  481 CATCCGTCGC TTACATGTGT TCCGCCACAC TTGCAACATC ACACTTCCGC CACACTACTA
  541 CGTCACCCGC CCCGTTCCCA CGCCCCGCGC CACGTCACAA ACTCCACCCC CTCATTATCA
  601 TATTGGCTTC AATCCAAAAT AAGGTATATT ATTGATGATG TTCGAATTAA TTAAATCGAA
  661 ATCGAACATG CGGATCGATC CGGAATTCTT GAAGACGAAA GGGCCTCGTG ATACGCCTAT
  721 TTTTATAGGT TAATGTCATG ATAATAATGG TTTCTTAGAC GTCAGGTGGC ACTTTTCGGG
  781 GAAATGTGCG CGGAACCCCT ATTTGTTTAT TTTTCTAAAT ACATTCAAAT ATGTATCCGC
  841 TCATGAGACA ATAACCCTGA TAAATGCTTC AATAATATTG AAAAAGGAAG AGTATGAGTA
  901 TTCAACATTT CCGTGTCGCC CTTATTCCCT TTTTTGCGGC ATTTTGCCTT CCTGTTTTTG
  961 CTCACCCAGA AACGCTGGTG AAAGTAAAAG ATGCTGAGAT CAGTTGGGGT GCACGAGTGG
 1021 GTTACATCGG AACTGGATCT CAACAGCGGT AGATCCTTTG AGAGTTTTTC GCCCCCGAGA
 1081 AACGTTTTCC ATGGATGGAG CCACTTTAAA GGTCTGCTAT GTGGGCGCGG GTTATTTATT
1141 CCCGCTGTTT GAACGACCCG CGCCAAGGAG AGGCCAAAAT
//
Primer: pAxcwit2-Rv RDB05212_A3Ahb.seq
>05212_05212_A3Ah_pAxcwit2-Rv_H08_23.ab1
    1 CATAAGATGA GTGAATCTGA ATAATTTTGT GTTACTCATA GCGCGTAATA TTTGTCTAGG
   61 GCCGCGGGGA CTTTGACCGT TTACGTGGAG ACTCGCCCAG GTGTTTTTCT CAGGTGTTTT
  121 CCGCGTTCCG GGTCAAAGTT GGCGTTTTAT TATTATAGTC AGCATCGATT TAAATCGATT
  181 GTCGACTCGC GACGATGGAT CTGGAAGGTG CTGAGGTACG ATGAGACCCG CACCAGGTGC
  241 AGACCCTGCG AGTGTGGCGG TAAACATATT AGGAACCAGC CTGTGATGCT GGATGTGACC
  301 GAGGAGCTGA GGCCCGATCA CTTGGTGCTG GCCTGCACCC GCGCTGAGTT TGGCTCTAGC
  361 GATGAAGATA CAGATTGAGG TACTGAAATG TGTGGGCGTG GCTTAAGGGT GGGAAAGAAT
  421 ATATAAGGTG GGGGTCTTAT GTAGTTTTGT ATCTGTTTTG CAGCAGCCGC CGCCGCCATG
  481 AGCACCAACT CGTTTGATGG AAGCATTGTG AGCTCATATT TGACAACGCG CATGCCCCCA
  541 TGGGCCGGGG TGCGTCAGAA TGTGATGGGC TCCAGCATTG ATGGTCGCCC CGTCCTGCCC
  601 GCAAACTCTA CTACCTTGAC CTACGAGACC GTGTCTGGAA CGCCGTTGGA GACTGCAGCC
  661 TCCGCCGCCG CTTCAGCCGC TGCAGCCACC GCCCGCGGGA TTGTGACTGA CTTTGCTTTC
  721 CTGAGCCCGC TTGCAAGCAG TGCAGCTTCC CGTTCATCCG CCCGCGATGA CAAGTTGACG
  781 GCTCTTTTGG CACAATTGGA TTCTTTGACC CGGGAACTTA ATGTCGTTTC TCAGCAGCTG
  841 TTGGATCTGC GCCAGCAGTT TCTGCCCTGA AAGCTTCCTC CCCTCCCAAT GCGGTTTAAA
  901 ACATAAATAA AAAACCAGAC TCTGTTTGGA TTTGGATCAA GCAAGTGTCT TGCTGTCTTT
  961 ATTTAGGGTT TTGCGCGCGC GGTAGCCCGG GACCAGCGGT CTCGGTCGTT GAGGGTCCTG
 1021 TGTATTTTTT TTCCAGGACG TGTAAAGGTG ACCTCTGGGA TGTTCAGAAT ACCATGGGCC
 1081 ATAAGCCCGT CCTCTTGGGG GGTGGAAGGT AGGCACCCAC TGCAGAGCTT CCATGGCTGC
1141 CGGGGTGGGG TGGTTTGGTT AGAAATGGAA TTTCCTCAG
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Fukuda, H., Possible mechanism of adenovirus generation from a cloned viral genome tagged with nucleotides at its ends. Microbiol. Immunol., 50, 643-654 (2006). PMID 16924150.
original Terashima, M., Exp. Med., 21, 931-936 (2003).

2018.12.10

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