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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

SEREX clone BRC-Co-27 #1 (#RDB04487)

SEREX clone BRC-Co-27 #1

Clone info. SEREX clone BRC-Co-27, clone 1 from Colon Cancer AGH-CO-2. Reactivity: +. Corresponding gene: Chromosome X Open Reading Frame 15 (Cxorf15). Nucleoide position 43nt to 4413nt of NM_018360.2 (cds 57-1643nt) is cloned. Known difference (at least): G to A substitution at 1382nt (synonym).
Comment Similar clone: 5': NGO-Br-30 5' (ID 840) 99% similarity, Hom-TsMe2-14-R (ID 1123) 98% similarity; 3': NGO-Br-30 3' (ID 841) 99% similarity, Hom-TsMe2-14-U (ID 1122) 98% similarity. UniGene cluster: 5': Hs. 201624, Limited tissues; 3':Hs. 406078, Ubiquitous.
Vector backbone pBK-CMV (Plasmid)
Size of vector backbone 4.5 kb
Selectable markers Kan^r
Gene/insert name Human Chromosome X Open Reading Frame 15 (Cxorf15) (NM_018360, 2045 bp) cDNA
Depositor|Developer Obata, Yuichi |
 

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RDB04487 SEREX clone BRC-Co-27 #1 DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The SEREX clone BRC-Co-27 #1 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB04487).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15326_A7Hfp1_2.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: RDB04487#2
Sequence file: RDB15326_A7Hfa_2.seq check
>04487_15326_A7Hf_RDB04487#2_A02_01_ABI08.ab1
    1 ACCCAAGGCC TAATGTTTTC CTGTCGGAGT TTGGCGTTGT GGATGTCATG CTGCTCCAGC
   61 TGGGCTTGAA TTTCATTTAA GGTAATCTGG AAATGTGCAG TTGCTTCTTT ACGTCGTTCT
  121 TCTTCCTCTC GTGCCTGCTG CATATTTTCC TCCTTTAACG TCTTATTGTG ACGCTGAAGT
  181 TCTCTGCAAA GAGATTCTAG CTTGCTTCTT GCCAAGATAG CCTTGCTATG TTCACTCTGC
  241 AAGTGAACTT TCTCTTTCAC AATCTGGGCT TGCTTCTTCT GCAGGATCTT CATTTGCTTC
  301 TGAACACTCC TGCTCTCCTC CAGAAGATCA GCATATTTCT TACAGAGAGC TGCCAGCTTC
  361 TCCTCTGGGG TTGAAAGGGT GTTTAGGGCT TGCATCAGTA ATAAAACTTC TTTTCCTAAA
  421 GTTTTTTCTT TGTTCCTGTT GCACTCTGAA TCTTGCTGAC CATCAGGGGG ATCTGTTCGA
  481 GCTTCTCCCC CAGGGATTTC CTCTCTTGAT TCTTGCGTGC AGTATGCTGG GCTCACCAAA
  541 TTCCTGTTCT CTGTTATAAA GTCACTGCCT TCATCCTCTT CCAATGAATG CTTGTTACTT
  601 GTGCCACCAC AATTTGAGCC TTGATGTTGA AGAATATCAT TTGATTGAGA GTTACACAAC
  661 ATATCTGCTT TCACCCCTAG CCCACAAATT CCAGCTTCTT CCATTGTGCC AATTTCAAAC
  721 TTCTGCCGCG GGCTGCGTCG CCGTCCGCCC CGTCCGGCCT CAGTCGCCTC TTCGGCGCCG
  781 CCGCCTCTTC CCCGCGCTGC CTCCTCTACC CGCGTCGCCA TGAGGTGACG GCAGCCTCGT
  841 GCCGAATTCT TTGGATCCAC TAGTGTCGAC CTGCAGGCGC GCGAGCTCCA GCTTTTGTTC
  901 CCTTTAGTGA GGGTTAATTT CGAGCTTGGC GTAATCAAAG GTCATAGCTG TTTCCTGTGT
  961 GAAATTGTTA TCCGCTCACA ATTCCACACA ATATACGAGC CGGAAGTATA AAGTGTAAAG
 1021 CCCTGGGGGT GCCTAATGAG TGAGCTAACT CACAGTAAAT TGCGGGCTAG CGGATCTGAC
 1081 GATTCACTAA ACCAGCTCTG GCTATATAGA CTTCCCACCG TACACGTCCT ACCGGCCCAA
1141 TTTTGGCGGT TCC
//
Primer: RDB04487#1
Sequence file: RDB15326_A7Hfb_2.seq check
>04487_15326_A7Hf_RDB04487#1_E01_05_ABI08.ab1
    1 CGGAAAATGG GTAGGAAAAA GTAAGGAAAA TTTTCACCAC AAAACCAATA GTTAACTACT
   61 AACAGAGAAA GTTATACACA AAATATATCT CTCAATACAG TGATCACACC TCATCTTAGT
  121 CAACCGACTC GATGGCCGGA GCAGAGCCTG TGGACGGGGG CTTTTGCACA GCGCTTCTCT
  181 GACTCTTGGG CTCAGCCTCC AGGTGCGCTC CCAGGGCTCT TTTCGAGGAA GTGTTCAGCT
  241 CCTTGTGAGA ATCCAGGGCA GTACAGGGCT GCATCACAGG TGTTGCTAAA TCCCTGTTCG
  301 CCGCTTTGAT GGCCGCTTTG ATGGATACCT GCTCTTTCAG GACTTCCACC TTCTCATTGA
  361 GCTCATTCCT TTCTGTTTGA AGAGCCCTGC ACAGCTTCTC TAACCGTTCC AGTTTTATTT
  421 GAAGGGCCTT GTACTCTTTA TCACGGACTG TTTTCTCTTC AGCCATTTGC AGAAGTGCTT
  481 TATTATTGTT TTCCCATTTG GTACGCCAAA TTATTGTTTC TTTTTCCAGT TTTTTAATTT
  541 TCTTTGTCAT CTTTTCCATT TCCTGTCTGA AGGTTGTAAA CAGTTCATTG CTTTTTGCCA
  601 TGGTAGTCTG GAATTCTTCA AACTTATCCA TATAAAGAGA AAGCTGCTGT TTTAGTTGTA
  661 CTTCTTGCTG TTTCATTTGT TCGTATTTGT GCCTCGATTC TGTCGCTTCT TTTAATAAAA
  721 ACTCTCTCTC TCTCTGATGT TTTTCATCAG CTTCTTTTAT CAGTTGTGTC GTTTGCTGCA
  781 GTTTGGCATC CACGAGCTGC TGTTGCAGTT CCTTATGTTT GAACACCTTA TCAATGTGCT
  841 CTTCCCTCAG TGCGTACTGT TCGATGAGCT TCTTTAGCTT CTCCCCCAGC TCAATGTTTT
  901 CCTGTCGGAG TTTGGCGTTG TGGATGTCAT GCTGCTCCAG CTGGGGCTTG AATTTCATTT
  961 AAGGTAATCT GGAAATGTGC AGTTGCTTCT TTACGTCGTT CTTCTTTCCT CTCGTGCCCT
 1021 GCTGCATATT TTCCTCCCTT TAACGTCTTA TTGTGACGCT GAAGTTCTCT GCAAAGAGAT
 1081 TCTAGCTTGC TTTCTTGCCA AGATAGCCCT TTGCTTATGT TCACTCTGCA GTGAACTTCT
1141 CTTTCACAAT CTTGGGGCCC TTTGCTTTTC TTTCTCGTGC A
//
Primer: SV40polyA-R
Sequence file: RDB15326_A7Hfc_2.seq check
>04487_15326_A7Hf_2_SV40polyA_R_B07_04_ABI24.ab1
    1 AAGAAATTGG AGTTTTAAGC AGTAAACCTC TACAAATGTG GTATGGCTGA TTATGATCAT
   61 GAACAGACTG TGAGGACTGA GGGGCCTGAA ATGAGCCTTG GGACTGTGAA TCTAAAATAC
  121 ACAAACAATT AGAATCAGTA GTTTAACACA TTATACACTT AAAAATTGGA TCTCCATTCG
  181 CCATTCAGGC TGCGCAACTG TTGGGAAGGG CGATCGGTGC GGGCCTCTTC GCTATTACGC
  241 CAGCTGGCGA AAGGGGGATG TGCTGCAAGG CGATTAAGTT GGGTAACGCC AGGGTTTTCC
  301 CAGTCACGAC GTTGTAAAAC GACGGCCAGT GAATTGTAAT ACGACTCACT ATAGGGCGAA
  361 TTGGGTACAC TTACCTGGTA CCCCACCCGG GTGGAAAATC GATGGGCCCG CGGCCGCTCT
  421 AGAAGTACTC TCGAGTTTTT TTTTTTTTTT TTTTAAGTTT ATCATACATA TTTACTTAAT
  481 CTATACAGAA TCAAGTAGAT AAAATCAGAT TCACATTAGT GAAAAATCTC TACAAAATGT
  541 CTTTGAAAAG CAAAACGAGA CTGCCTCTTA ACAATTCGTA TCCTCATGAA AGACGTGGGC
  601 TGTAAAAATA AAGCAGTGTG TTTAGCACAA ACTAGAAGAT ATCTGCATCA TTTTTTTCAC
  661 AGTTATTTCC ATCTACAGTC TGAAAGAGGT AGATTTTTCT GCAACACCTG AGAATTGAAT
  721 TGTGATAACC AGGTGTAATA AAGGCAGTTG CATACATAAC TGAAAAATAC TGGTCTCAAG
  781 ACCAAGAAAT GTACTCCTAA CAAGTCTGAG GATGAAATGG TCCATATTAC CCAAGACCCA
  841 AGTTTTCTCT ACACCTTGGA GTCCACCGCC AATATAAATA ATACACATCA CGATTCCATG
  901 ATGGTGCAAC AATTGCAATT TAAGTGAATT CAACATTTAA ACCTTTTCTG GGCCCAGCCT
  961 AGTAGCATTA CAGTGTTCTA AGAAATATGG TAGATATGGG GGTGCGCTAC AAAGCCACAC
 1021 ACTGGGCTGT GAGTGATCAC TGTAGGAAGA TGTCTCTCAG GGGTCCATTG TTTTTATTCA
 1081 CTGTATTGGA GAGGATCTGG AAGACACAAT GCATTCAGAC AGAATTCAAT ATCGTTCCCA
1141 ACCACTATAA AATATAAG
//
Primer: SV40pro_F_V2
Sequence file: RDB15326_A7Hfd_2.seq check
>04487_15326_A7Hf_2_SV40pro_F_V2_C07_07_ABI24.ab1
    1 TGTCCGAGCG TTTTTGGAGC TAGGCTTTTG CAAGATCGAT CAAGAGACAG GATGAGGATC
   61 GTTTCGCATG ATTGAACAAG ATGGATTGCA CGCAGGTTCT CCGGCCGCTT GGGTGGAGAG
  121 GCTATTCGGC TATGACTGGG CACAACAGAC AATCGGCTGC TCTGATGCCG CCGTGTTCCG
  181 GCTGTCAGCG CAGGGGCGCC CGGTTCTTTT TGTCAAGACC GACCTGTCCG GTGCCCTGAA
  241 TGAACTGCAA GACGAGGCAG CGCGGCTATC GTGGCTGGCC ACGACGGGCG TTCCTTGCGC
  301 AGCTGTGCTC GACGTTGTCA CTGAAGCGGG AAGGGACTGG CTGCTATTGG GCGAAGTGCC
  361 GGGGCAGGAT CTCCTGTCAT CTCACCTTGC TCCTGCCGAG AAAGTATCCA TCATGGCTGA
  421 TGCAATGCGG CGGCTGCATA CGCTTGATCC GGCTACCTGC CCATTCGACC ACCAAGCGAA
  481 ACATCGCATC GAGCGAGCAC GTACTCGGAT GGAAGCCGGT CTTGTCGATC AGGATGATCT
  541 GGACGAAGAG CATCAGGGGC TCGCGCCAGC CGAACTGTTC GCCAGGCTCA AGGCGAGCAT
  601 GCCCGACGGC GAGGATCTCG TCGTGACCCA TGGCGATGCC TGCTTGCCGA ATATCATGGT
  661 GGAAAATGGC CGCTTTTCTG GATTCATCGA CTGTGGCCGG CTGGGTGTGG CGGACCGCTA
  721 TCAGGACATA GCGTTGGCTA CCCGTGATAT TGCTGAAGAG CTTGGCGGCG AATGGGCTGA
  781 CCGCTTCCTC GTGCTTTACG GTATCGCCGC TCCCGATTCG CAGCGCATCG CCTTCTATCG
  841 CCTTCTTGAC GAGTTTCTTC TGAGCGGGAC TCTGGGGTTC GAAATGACCG ACCAAGCGAC
  901 GCCCAACCTG CCATCACGAG ATTTCGATTC CACCGCCGCC CTTCTATGAA AGTTGGCTTC
  961 GGAATCGTTT TTCCGGGACG CCCGGCTGGG ATGATCCTTC CAGCGCGGGG GATTCTTCAT
 1021 GCTTGGAGTT CTTTCGCCCA ACCCTTAGGG GGGGAGGCTT AACTGAACAA CGAGAGACCA
 1081 ATTACCCGGG AAGGAAACCG CGCTATGACG CCAATTAAAA GACGGATTAA ACGCACGTGT
1141 TTGGGTCGTT TGGTTCATAT AACGCCGGGG TTCGTTCTCC TCAAGG
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles