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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pENTR4-H1 (#RDB04395)

Expression vector of RNAi driven by human H1 promoter.

Clone info. Expression vector of RNAi driven by human H1 promoter. Frequently requested along with Gateway(R) destination vectors for constructing short hairpin RNA (shRNA) expressing vectors.
Vector backbone pENTR4 (Plasmid)
Selectable markers Kan^r
Gene/insert name Human RPPH1 genomic DNA
Depositor|Developer Miyoshi, Hiroyuki |
 
Sequence RDB04395z.seq
Remarks, protocol and/or map (pdf) RDB04395.pdf

Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 本件研究材料は、非営利機関の非営利学術研究に限って提供する。本件研究材料を利用した研究結果等を発表する際は、本件研究材料が故三好浩之博士により開発されたことを明示する。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB04395 pENTR4-H1 DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pENTR4-H1 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB04395).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB08058_A5Bcp1-5.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: KanR-N_R (Pr0530)
Region: attL2,H1 pro,attL1,rrn T2 ter
Sequence file: RDB08058_A5Bcc.seq check
>04395_08058_A9FpF_KanR_H06_24_ABI24.ab1
    1 NNNNNNNNNN NNNNNATAAN TNNGCATCCN TGTTGGAATT TAATCGCGGC CTCGACGTTT
   61 CCCGTTGAAT ATGGCTCATA ACACCCCTTG TATTACTGTT TATGTAAGCA GACAGTTTTA
  121 TTGTTCATGA TGATATATTT TTATCTTGTG CAATGTAACA TCAGAGATTT TGAGACACGG
  181 GCCAGAGCTG CAGCTGGATG GCAAATAATG ATTTTATTTT GACTGATAGT GACCTGTTCG
  241 TTGCAACAAA TTGATAAGCA ATGCTTTCTT ATAATGCCAA CTTTGTACAA GAAAGCTGGG
  301 TCTAGATATC TCGACTCTAG AGGATCCCCG GCAGATCTGT GGTCTCATAC AGAACTTATA
  361 AGATTCCCAA ATCCAAAGAC ATTTCACGTT TATGGTGATT TCCCAGAACA CATAGCGACA
  421 TGCAAATATT GCAGGGCGCC ACTCCCCTGT CCCTCACAGC CATCTTCCTG CCAGGGCGCA
  481 CGCGCGCTGG GTGTTCCCGC CTAGTGACAC TGGGCCCGCG ATTCCTTGGA GCGGGTTGAT
  541 GACGTCAGCG TTCGAATTCG GTACCGGATC CAGTCGACTG AATTGGTTCC CATGGTGGAG
  601 CCTGCTTTTT TGTACAAAGT TGGCATTATA AAAAAGCATT GCTTATCAAT TTGTTGCAAC
  661 GAACAGGTCA CTATCAGTCA AAATAAAATC ATTATTTGGG GCCCGAGCTT AAGTAACTAA
  721 CTAACAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA
  781 GTTTGATGCC TGGCAGTTTA TGGCGNGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC
  841 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA NGANANCGTT CACNGACAAA
  901 CAACANATAA AACGAAAGGC CNNNNCNNNC NNNNGANCNN TTCNNTTTAT TNGATGCNNG
961 GNANNNCCNN NNNNNNGCNN ANNANNNNNN NNNN
//
Primer: pDONR_R (Pr0071)
Region: KanR,attL2,H1 pro,attL1,rrn T2 ter
Sequence file: RDB08058_A5Bcd.seq check
>04395_08058_A5Bc_pDONR.R_H10_22.ab1
    1 NNNNNNNNNN NNNNNNNNNG NNNNNNNGNN TTNNTTTTGN NTNNNANNGN NCNGTTCGTT
   61 GCAACAAATT GATAAGCAAT GCTTTCTTAT AATGCCAACT TTGTACAAGA AAGCTGGGTC
  121 TAGATATCTC GACTCTAGAG GATCCCCGGC AGATCTGTGG TCTCATACAG AACTTATAAG
  181 ATTCCCAAAT CCAAAGACAT TTCACGTTTA TGGTGATTTC CCAGAACACA TAGCGACATG
  241 CAAATATTGC AGGGCGCCAC TCCCCTGTCC CTCACAGCCA TCTTCCTGCC AGGGCGCACG
  301 CGCGCTGGGT GTTCCCGCCT AGTGACACTG GGCCCGCGAT TCCTTGGAGC GGGTTGATGA
  361 CGTCAGCGTT CGAATTCGGT ACCGGATCCA GTCGACTGAA TTGGTTCCCA TGGTGGAGCC
  421 TGCTTTTTTG TACAAAGTTG GCATTATAAA AAAGCATTGC TTATCAATTT GTTGCAACGA
  481 ACAGGTCACT ATCAGTCAAA ATAAAATCAT TATTTGGGGC CCGAGCTTAA GTAACTAACT
  541 AACAGGAAGA GTTTGTAGAA ACGCAAAAAG GCCATCCGTC AGGANGGCCT TCTGCTTAGT
  601 TTGANGCCTG GCAGTTTATG GCGGGCGTCC TGCCCGCCAC CCTCCGGGCC GTTGCTTCNC
  661 AACGTTCAAA TCNNCTCCCG GCGNNNTTGT CCTACTCNNG ANANCGNTCA CCGANAAACA
  721 ACAGANAAAA CGAAANGNCC AGNCNNNCNA CNGANCNTTT NNNNTTANTT GANGNNNNNN
  781 NNTTCCCTAC TCNCNCTNAN NAGTTANNNG NNCCCNNNNN NNNNGCTANN NGNNNNNNNG
841 NNNNNNNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

user_report Yamagishi, M., Mechanisms of action and resistance in histone methylation-targeted therapy. Nature 627 (8002): 221-228 (2024). PMID 38383791. [link to RRC of NBRP]
user_report Yang, S., Neutrophil Extracellular Traps Delay Diabetic Wound Healing by Inducing Endothelial-to-Mesenchymal Transition via the Hippo pathway. Int J Biol Sci. 2023 Jan 1;19(1):347-361 (2023). PMID 36594092. [link to RRC of NBRP]
user_report Araki, H., LSD1 defines the fiber type-selective responsiveness to environmental stress in skeletal muscle. Elife 12: e84618 (2023). PMID 36695573. [link to RRC of NBRP]
user_report Watanabe, H., Astrocytic APOE4 genotype-mediated negative impacts on synaptic architecture in human pluripotent stem cell model. Stem Cell Reports 18 (9): 1854-1869 (2023). PMID 37657448. [link to RRC of NBRP]
user_report Truong, T.T.K., Arl4c is involved in tooth germ development through osteoblastic/ameloblastic differentiation. Biochem. Biophys. Res. Commun. 679: 167-174 (2023). PMID 37703759. [link to RRC of NBRP]
user_report Shimizu, K., Diurnal variation in declarative memory and the involvement of SCOP in cognitive functions in nonhuman primates. 02 November 2022, PREPRINT (Version 1) available at Research Square https://doi.org/10.21203/rs.3.rs-2013762/v1
user_report Miyakuni, K., Genome-wide analysis of DNA methylation identifies the apoptosis-related gene UQCRH as a tumor suppressor in renal cancer. Mol. Oncol. 16 (3): 732-749 (2022). PMID 34133843. [link to RRC of NBRP]
user_report Funaki, T., CADM1 promotes malignant features of small-cell lung cancer by recruiting 4.1R to the plasma membrane. Biochem. Biophys. Res. Commun. 534: 172-178 (2021). PMID 33298314. [link to RRC of NBRP]
user_report Totani, H., Autocrine HGF/c-Met signaling pathway confers aggressiveness in lymph node adult T-cell leukemia/lymphoma. Oncogene 39 (35): 5782-5794 (2020). PMID 32747750. [link to RRC of NBRP]
user_report Yuki, R., Desuppression of TGF-beta signaling via nuclear c-Abl-mediated phosphorylation of TIF1 gamma/TRIM33 at Tyr-524, -610, and -1048. Oncogene 38 (5): 637-655 (2019). PMID 30177833. [link to RRC of NBRP]
user_report Ito, T., Mathematical analysis of gefitinib resistance of lung adenocarcinoma caused by MET amplification. Biochem. Biophys. Res. Commun. 511 (3): 544-550 (2019). PMID 30824185. [link to RRC of NBRP]
user_report Nakajo, H., Role for tyrosine phosphorylation of SUV39H1 histone methyltransferase in enhanced trimethylation of histone H3K9 via neuregulin-1/ErbB4 nuclear signaling. Biochem. Biophys. Res. Commun. 511 (4): 765-771 (2019). PMID 30833073. [link to RRC of NBRP]
user_report Kamil, M., High filamin-C expression predicts enhanced invasiveness and poor outcome in glioblastoma multiforme. Br. J. Cancer. 120 (8): 819-826 (2019). PMID 30867563. [link to RRC of NBRP]
user_report Makino, Y., Single cell RNA-sequencing identified Dec2 as a suppressive factor for spermatogonial differentiation by inhibiting Sohlh1 expression. Sci. Rep. 9 (1): 6063 (2019). PMID 30988352. [link to RRC of NBRP]
user_report Yamagishi, M., Targeting Excessive EZH1 and EZH2 Activities for Abnormal Histone Methylation and Transcription Network in Malignant Lymphomas. Cell Rep. 29 (8): 2321-2337.e7 (2019). PMID 31747604. [link to RRC of NBRP]
user_report Jinnou, H., Radial Glial Fibers Promote Neuronal Migration and Functional Recovery after Neonatal Brain Injury. Cell Stem Cell 22 (1): 128-137.e9. (2018). PMID 29276142. [link to RRC of NBRP]
user_report Kawasaki, N., TUFT1 interacts with RABGAP1 and regulates mTORC1 signaling Cell Discovery 4 (1): (2018). PMID 29423269. [link to RRC of NBRP]
user_report Funato, K., SIRT2-mediated inactivation of p73 is required for glioblastoma tumorigenicity. EMBO Rep. 19 (11). pii: e45587 (2018). PMID 30213795. [link to RRC of NBRP]
user_report Neri, S., Fibroblast-led cancer cell invasion is activated by epithelial-mesenchymal transition through platelet-derived growth factor BB secretion of lung adenocarcinoma. Cancer Lett. 395: 20-30 (2017). PMID 28286261. [link to RRC of NBRP]
user_report Ishibashi, M., CD200-positive cancer associated fibroblasts augment the sensitivity of Epidermal Growth Factor Receptor mutation-positive lung adenocarcinomas to EGFR Tyrosine kinase inhibitors. Sci. Rep. 7: 46662 (2017). PMID 28429795. [link to RRC of NBRP]
user_report Katsura, A., MicroRNA-31 is a positive modulator of endothelial-mesenchymal transition and associated secretory phenotype induced by TGF-beta. Genes Cells 21 (1): 99-116 PMID 26663584. [link to RRC of NBRP]
user_report Takahashi, Y., Perilipin2 plays a positive role in adipocytes during lipolysis by escaping proteasomal degradation. Sci Rep. 6: 20975 (2016). PMID 26876687. [link to RRC of NBRP]
user_report Hikasa, H., Merlin/NF2-Lin28B-let-7 Is a Tumor-Suppressive Pathway that Is Cell-Density Dependent and Hippo Independent. Cell Rep. 14 (12): 2950-2961 (2016). PMID 26997273. [link to RRC of NBRP]
user_report Johmura, Y., Defective DNA repair increases susceptibility to senescence through extension of Chk1-mediated G2 checkpoint activation. Sci. Rep. 6: 31194 (2016). PMID 27507734. [link to RRC of NBRP]
user_report Inoue, J., Identification of BCL11B as a regulator of adipogenesis. Sci. Rep. 6: 32750 (2016). PMID 27586877. [link to RRC of NBRP]
user_report Matsuda, Y., Epigenetic Heterogeneity in HIV-1 Latency Establishment. Sci. Rep. 5: 7701 (2015). PMID 25572573. [link to RRC of NBRP]
user_report Neri, S., Podoplanin-expressing cancer-associated fibroblasts lead and enhance the local invasion of cancer cells in lung adenocarcinoma. Int. J. Cancer. 137 (4): 784-796 (2015). PMID 25648219. [link to RRC of NBRP]
user_report Yamagishi M, Polycomb-Mediated Loss of miR-31 Activates NIK-Dependent NF-κB Pathway in Adult T Cell Leukemia and Other Cancers. Cancer Cell, 21 (1): 121-135 (2012). PMID 22264793. [link to RRC of NBRP]
user_report Sasaki, M., Equine major histocompatibility complex class I molecules act as entry receptors that bind to equine herpesvirus-1 glycoprotein D. Genes Cells, 16 (4), 343-357 (2011). PMID 21306483. [link to RRC of NBRP]
user_report Aoyama, K., Nuclear c-Abl-mediated tyrosine phosphorylation induces chromatin structural changes through histone modifications that include H4K16 hypoacetylation. Exp. Cell Res., 317 (20): 2874-903 (2011). PMID 22001646. [link to RRC of NBRP]
user_report Tanaka, Y., Dual Function of Histone H3 Lysine 36 Methyltransferase ASH1 in Regulation of Hox Gene Expression. PLoS One., 6 (11): e28171 (2011). PMID 22140534. [link to RRC of NBRP]
user_report Ohoka, N., The orphan nuclear receptor RORalpha restrains adipocyte differentiation through a reduction of C/EBPbeta activity and perilipin gene expression. Mol. Endocrinol., 23, 759-771 (2009). PMID 19324970. [link to RRC of NBRP]
user_report Nakayama Y., Bleomycin-induced over-replication involves sustained inhibition of mitotic entry through the ATM/ATR pathway. Exp. Cell Res., 315, 2515-2528 (2009). PMID 19527713. [link to RRC of NBRP]
user_report Tanabe, R., PRRX1 induced by BMP signaling decreases tumorigenesis by epigenetically regulating glioma-initiating cell properties via DNA methyltransferase 3A. Mol. Oncol. 16 (1): 269-288 (2022). PMID 34214250. [link to RRC of NBRP]