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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pENTR4-H1

Expression vector of RNAi driven by human H1 promoter.

Catalog number RDB04395
Resource name pENTR4-H1
Clone info. Expression vector of RNAi driven by human H1 promoter. Frequently requested along with Gateway(R) destination vectors for constructing short hairpin RNA (shRNA) expressing vectors.
Vector backbone pENTR4 (Plasmid)
Selectable markers Kan^r
Gene/insert name Human RPPH1 genomic DNA
Depositor|Developer Miyoshi, Hiroyuki |
Other clones in our bank

External Database
Human RPPH1

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer.
          Ordering Please visit Information of Request for Distribution.[link] 
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          Remarks
          Gateway® Entry clone. Please refer Life Technologies Corporation signs license agreement with RIKEN BRC.
          提供案内 (日本国内) [open/close]

          提供条件 本件研究材料は、非営利機関の非営利学術研究に限って提供する。本件研究材料を利用した研究結果等を発表する際は、本件研究材料が故三好浩之博士により開発されたことを明示する。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
          備考
          Gateway®テクノロジーを用いたエントリークローンです。「Gateway®テクノロジーを用いたクローンの提供と寄託のご案内」をご覧ください。

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB04395 pENTR4-H1 DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Sequence RDB04395z.seq
          Remarks, protocol and/or map (pdf) RDB04395.pdf

          Original reference

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content Empty Backbone (English text)
          Featured content Empty Backbone (Japanese text)
          Featured content Lentivirus Vector (English text)
          Featured content Lentivirus Vector (Japanese text)

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB08058_A5Bcp1-4.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: KanR-N_R
          Region: attL2,H1 pro,attL1,rrn T2 ter
          Sequence file: RDB08058_A5Bcc.seq check
          >04395_08058_A9FpF_KanR_H06_24_ABI24.ab1
              1 NNNNNNNNNN NNNNNATAAN TNNGCATCCN TGTTGGAATT TAATCGCGGC CTCGACGTTT
             61 CCCGTTGAAT ATGGCTCATA ACACCCCTTG TATTACTGTT TATGTAAGCA GACAGTTTTA
            121 TTGTTCATGA TGATATATTT TTATCTTGTG CAATGTAACA TCAGAGATTT TGAGACACGG
            181 GCCAGAGCTG CAGCTGGATG GCAAATAATG ATTTTATTTT GACTGATAGT GACCTGTTCG
            241 TTGCAACAAA TTGATAAGCA ATGCTTTCTT ATAATGCCAA CTTTGTACAA GAAAGCTGGG
            301 TCTAGATATC TCGACTCTAG AGGATCCCCG GCAGATCTGT GGTCTCATAC AGAACTTATA
            361 AGATTCCCAA ATCCAAAGAC ATTTCACGTT TATGGTGATT TCCCAGAACA CATAGCGACA
            421 TGCAAATATT GCAGGGCGCC ACTCCCCTGT CCCTCACAGC CATCTTCCTG CCAGGGCGCA
            481 CGCGCGCTGG GTGTTCCCGC CTAGTGACAC TGGGCCCGCG ATTCCTTGGA GCGGGTTGAT
            541 GACGTCAGCG TTCGAATTCG GTACCGGATC CAGTCGACTG AATTGGTTCC CATGGTGGAG
            601 CCTGCTTTTT TGTACAAAGT TGGCATTATA AAAAAGCATT GCTTATCAAT TTGTTGCAAC
            661 GAACAGGTCA CTATCAGTCA AAATAAAATC ATTATTTGGG GCCCGAGCTT AAGTAACTAA
            721 CTAACAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA
            781 GTTTGATGCC TGGCAGTTTA TGGCGNGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC
            841 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA NGANANCGTT CACNGACAAA
            901 CAACANATAA AACGAAAGGC CNNNNCNNNC NNNNGANCNN TTCNNTTTAT TNGATGCNNG
          961 GNANNNCCNN NNNNNNGCNN ANNANNNNNN NNNN
          //
          Primer: pDONR_R
          Region: KanR,attL2,H1 pro,attL1,rrn T2 ter
          Sequence file: RDB08058_A5Bcd.seq check
          >04395_08058_A5Bc_pDONR.R_H10_22.ab1
              1 NNNNNNNNNN NNNNNNNNNG NNNNNNNGNN TTNNTTTTGN NTNNNANNGN NCNGTTCGTT
             61 GCAACAAATT GATAAGCAAT GCTTTCTTAT AATGCCAACT TTGTACAAGA AAGCTGGGTC
            121 TAGATATCTC GACTCTAGAG GATCCCCGGC AGATCTGTGG TCTCATACAG AACTTATAAG
            181 ATTCCCAAAT CCAAAGACAT TTCACGTTTA TGGTGATTTC CCAGAACACA TAGCGACATG
            241 CAAATATTGC AGGGCGCCAC TCCCCTGTCC CTCACAGCCA TCTTCCTGCC AGGGCGCACG
            301 CGCGCTGGGT GTTCCCGCCT AGTGACACTG GGCCCGCGAT TCCTTGGAGC GGGTTGATGA
            361 CGTCAGCGTT CGAATTCGGT ACCGGATCCA GTCGACTGAA TTGGTTCCCA TGGTGGAGCC
            421 TGCTTTTTTG TACAAAGTTG GCATTATAAA AAAGCATTGC TTATCAATTT GTTGCAACGA
            481 ACAGGTCACT ATCAGTCAAA ATAAAATCAT TATTTGGGGC CCGAGCTTAA GTAACTAACT
            541 AACAGGAAGA GTTTGTAGAA ACGCAAAAAG GCCATCCGTC AGGANGGCCT TCTGCTTAGT
            601 TTGANGCCTG GCAGTTTATG GCGGGCGTCC TGCCCGCCAC CCTCCGGGCC GTTGCTTCNC
            661 AACGTTCAAA TCNNCTCCCG GCGNNNTTGT CCTACTCNNG ANANCGNTCA CCGANAAACA
            721 ACAGANAAAA CGAAANGNCC AGNCNNNCNA CNGANCNTTT NNNNTTANTT GANGNNNNNN
            781 NNTTCCCTAC TCNCNCTNAN NAGTTANNNG NNCCCNNNNN NNNNGCTANN NGNNNNNNNG
          841 NNNNNNNNNN
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          user_report Funaki, T., CADM1 promotes malignant features of small-cell lung cancer by recruiting 4.1R to the plasma membrane. Biochem. Biophys. Res. Commun. 534: 172-178 (2021). PMID 33298314.
          user_report Miyakuni, K., Genome-wide analysis of DNA methylation identifies the apoptosis-related gene UQCRH as a tumor suppressor in renal cancer. Mol Oncol. 2021 Jun 16. doi: 10.1002/1878-0261.13040. Epub ahead of print. PMID 34133843.
          user_report Yuki, R., Desuppression of TGF-beta signaling via nuclear c-Abl-mediated phosphorylation of TIF1 gamma/TRIM33 at Tyr-524, -610, and -1048. Oncogene 38 (5): 637-655 (2019). PMID 30177833.
          user_report Ito, T., Mathematical analysis of gefitinib resistance of lung adenocarcinoma caused by MET amplification. Biochem. Biophys. Res. Commun. 511 (3): 544-550 (2019). PMID 30824185.
          user_report Nakajo, H., Role for tyrosine phosphorylation of SUV39H1 histone methyltransferase in enhanced trimethylation of histone H3K9 via neuregulin-1/ErbB4 nuclear signaling. Biochem. Biophys. Res. Commun. 511 (4): 765-771 (2019). PMID 30833073.
          user_report Kamil, M., High filamin-C expression predicts enhanced invasiveness and poor outcome in glioblastoma multiforme. Br. J. Cancer. 120 (8): 819-826 (2019). PMID 30867563.
          user_report Makino, Y., Single cell RNA-sequencing identified Dec2 as a suppressive factor for spermatogonial differentiation by inhibiting Sohlh1 expression. Sci. Rep. 9 (1): 6063 (2019). PMID 30988352.
          user_report Yamagishi, M., Targeting Excessive EZH1 and EZH2 Activities for Abnormal Histone Methylation and Transcription Network in Malignant Lymphomas. Cell Rep. 29 (8): 2321-2337.e7 (2019). PMID 31747604.
          user_report Jinnou, H., Radial Glial Fibers Promote Neuronal Migration and Functional Recovery after Neonatal Brain Injury. Cell Stem Cell 22 (1): 128-137.e9. (2018). PMID 29276142.
          user_report Kawasaki, N., TUFT1 interacts with RABGAP1 and regulates mTORC1 signaling Cell Discovery 4 (1): (2018). PMID 29423269.
          user_report Funato, K., SIRT2-mediated inactivation of p73 is required for glioblastoma tumorigenicity. EMBO Rep. 19 (11). pii: e45587 (2018). PMID 30213795.
          user_report Neri, S., Fibroblast-led cancer cell invasion is activated by epithelial-mesenchymal transition through platelet-derived growth factor BB secretion of lung adenocarcinoma. Cancer Lett. 395: 20-30 (2017). PMID 28286261.
          user_report Ishibashi, M., CD200-positive cancer associated fibroblasts augment the sensitivity of Epidermal Growth Factor Receptor mutation-positive lung adenocarcinomas to EGFR Tyrosine kinase inhibitors. Sci. Rep. 7: 46662 (2017). PMID 28429795.
          user_report Katsura, A., MicroRNA-31 is a positive modulator of endothelial-mesenchymal transition and associated secretory phenotype induced by TGF-beta. Genes Cells 21 (1): 99-116 PMID 26663584.
          user_report Takahashi, Y., Perilipin2 plays a positive role in adipocytes during lipolysis by escaping proteasomal degradation. Sci Rep. 6: 20975 (2016). PMID 26876687.
          user_report Hikasa, H., Merlin/NF2-Lin28B-let-7 Is a Tumor-Suppressive Pathway that Is Cell-Density Dependent and Hippo Independent. Cell Rep. 14 (12): 2950-2961 (2016). PMID 26997273.
          user_report Johmura, Y., Defective DNA repair increases susceptibility to senescence through extension of Chk1-mediated G2 checkpoint activation. Sci. Rep. 6: 31194 (2016). PMID 27507734.
          user_report Inoue, J., Identification of BCL11B as a regulator of adipogenesis. Sci. Rep. 6: 32750 (2016). PMID 27586877.
          user_report Matsuda, Y., Epigenetic Heterogeneity in HIV-1 Latency Establishment. Sci. Rep. 5: 7701 (2015). PMID 25572573.
          user_report Neri, S., Podoplanin-expressing cancer-associated fibroblasts lead and enhance the local invasion of cancer cells in lung adenocarcinoma. Int. J. Cancer. 137 (4): 784-796 (2015). PMID 25648219.
          user_report Yamagishi M, Polycomb-Mediated Loss of miR-31 Activates NIK-Dependent NF-κB Pathway in Adult T Cell Leukemia and Other Cancers. Cancer Cell, 21 (1): 121-135 (2012). PMID 22264793.
          user_report Sasaki, M., Equine major histocompatibility complex class I molecules act as entry receptors that bind to equine herpesvirus-1 glycoprotein D. Genes Cells, 16 (4), 343-357 (2011). PMID 21306483.
          user_report Aoyama, K., Nuclear c-Abl-mediated tyrosine phosphorylation induces chromatin structural changes through histone modifications that include H4K16 hypoacetylation. Exp. Cell Res., 317 (20): 2874-903 (2011). PMID 22001646.
          user_report Tanaka, Y., Dual Function of Histone H3 Lysine 36 Methyltransferase ASH1 in Regulation of Hox Gene Expression. PLoS One., 6 (11): e28171 (2011). PMID 22140534.
          user_report Ohoka, N., The orphan nuclear receptor RORalpha restrains adipocyte differentiation through a reduction of C/EBPbeta activity and perilipin gene expression. Mol. Endocrinol., 23, 759-771 (2009). PMID 19324970.
          user_report Nakayama Y., Bleomycin-induced over-replication involves sustained inhibition of mitotic entry through the ATM/ATR pathway. Exp. Cell Res., 315, 2515-2528 (2009). PMID 19527713.

          2022.06.17

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