Resource data sheet
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pENTR4-H1

Expression vector of RNAi driven by human H1 promoter.

Catalog number RDB04395
Resource name pENTR4-H1
Clone info. Expression vector of RNAi driven by human H1 promoter. Frequently requested along with Gateway(R) destination vectors for constructing short hairpin RNA (shRNA) expressing vectors.
Vector backbone Plasmid
Selectable markers Kan^r
Gene/insert name Vector
Originator Miyoshi, Hiroyuki |

Distribution information

CLICK here for plasmid list and map. [link]
Forms for plasmid request [in Japanese] [in English]
Terms and conditions for distribution The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose.
(Japanese text [open/close])

Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB08058_A5Bcp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pDONR_R RDB08058_A5Bca.seq
>04395_08058_A5Bc_pDONR.R_H10_22.ab1
    1 GGCAGACGTC AGCTGGATGG CAATAATGAT TTTATTTTGA CTGATAGTGA CCTGTTCGTT
   61 GCAACAAATT GATAAGCAAT GCTTTCTTAT AATGCCAACT TTGTACAAGA AAGCTGGGTC
  121 TAGATATCTC GACTCTAGAG GATCCCCGGC AGATCTGTGG TCTCATACAG AACTTATAAG
  181 ATTCCCAAAT CCAAAGACAT TTCACGTTTA TGGTGATTTC CCAGAACACA TAGCGACATG
  241 CAAATATTGC AGGGCGCCAC TCCCCTGTCC CTCACAGCCA TCTTCCTGCC AGGGCGCACG
  301 CGCGCTGGGT GTTCCCGCCT AGTGACACTG GGCCCGCGAT TCCTTGGAGC GGGTTGATGA
  361 CGTCAGCGTT CGAATTCGGT ACCGGATCCA GTCGACTGAA TTGGTTCCCA TGGTGGAGCC
  421 TGCTTTTTTG TACAAAGTTG GCATTATAAA AAAGCATTGC TTATCAATTT GTTGCAACGA
  481 ACAGGTCACT ATCAGTCAAA ATAAAATCAT TATTTGGGGC CCGAGCTTAA GTAACTAACT
  541 AACAGGAAGA GTTTGTAGAA ACGCAAAAAG GCCATCCGTC AGGATGGCCT TCTGCTTAGT
  601 TTGATGCCTG GCAGTTTATG GCGGGCGTCC TGCCCGCCAC CCTCCGGGCC GTTGCTTCAC
  661 AACGTTCAAA TCCGCTCCCG GCGGATTTGT CCTACTCAGG AGAGCGTTCA CCGACAAACA
  721 ACAGATAAAA CGAAAGGCCC AGTCTTCCGA CTGAGCCTTT CGTTTTATTT GATGCCTGGC
  781 AGTTCCCTAC TCTCGCTTAG TAGTTAGACG TCCCCGAGAT CCATGCTAGC GGTAATACGG
  841 GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAAACAT GTGAGCAAAA GGCCAGCAAA
  901 AGGCCAGGAA CCGTAAAATG GCCGCGTTTG CTGGCGTTTT TCATAGGCTC CCGCCCCCCT
  961 GACGAGCAAT CACAAAAATC GGACGCCTCA AGGTCAGAAG GTGGCGAAGC CGACAGAACC
1021 TATAAAGATC AGCGTTCCCT GAGCTTCC
//

Please visit Sequencing and PCR primers for primer information.

References and tips

Electronic file

References

user report Jinnou, H., Radial Glial Fibers Promote Neuronal Migration and Functional Recovery after Neonatal Brain Injury. Cell Stem Cell 22 (1): 128-137.e9. (2018). PMID 29276142.
user report Kawasaki, N., TUFT1 interacts with RABGAP1 and regulates mTORC1 signaling Cell Discovery 4 (1): (2018). PMID 29423269.
user report Neri, S., Fibroblast-led cancer cell invasion is activated by epithelial-mesenchymal transition through platelet-derived growth factor BB secretion of lung adenocarcinoma. Cancer Lett. 395: 20-30 (2017). PMID 28286261.
user report Ishibashi, M., CD200-positive cancer associated fibroblasts augment the sensitivity of Epidermal Growth Factor Receptor mutation-positive lung adenocarcinomas to EGFR Tyrosine kinase inhibitors. Sci. Rep. 7: 46662 (2017). PMID 28429795.
user report Katsura, A., MicroRNA-31 is a positive modulator of endothelial-mesenchymal transition and associated secretory phenotype induced by TGF-beta. Genes Cells 21 (1): 99-116 PMID 26663584.
user report Hikasa, H., Merlin/NF2-Lin28B-let-7 Is a Tumor-Suppressive Pathway that Is Cell-Density Dependent and Hippo Independent. Cell Rep. 14 (12): 2950-2961 (2016). PMID 26997273.
user report Neri, S., Podoplanin-expressing cancer-associated fibroblasts lead and enhance the local invasion of cancer cells in lung adenocarcinoma. Int. J. Cancer. 137 (4): 784-796 (2015). PMID 25648219.
user report Yamagishi M, Polycomb-Mediated Loss of miR-31 Activates NIK-Dependent NF-κB Pathway in Adult T Cell Leukemia and Other Cancers. Cancer Cell, 21 (1): 121-135 (2012). PMID 22264793.
user report Sasaki, M., Equine major histocompatibility complex class I molecules act as entry receptors that bind to equine herpesvirus-1 glycoprotein D. Genes Cells, 16 (4), 343-357 (2011). PMID 21306483.
user report Aoyama, K., Nuclear c-Abl-mediated tyrosine phosphorylation induces chromatin structural changes through histone modifications that include H4K16 hypoacetylation. Exp. Cell Res., 317 (20): 2874-903 (2011). PMID 22001646.
user report Tanaka, Y., Dual Function of Histone H3 Lysine 36 Methyltransferase ASH1 in Regulation of Hox Gene Expression. PLoS One., 6 (11): e28171 (2011). PMID 22140534.
user report Ohoka, N., The orphan nuclear receptor RORalpha restrains adipocyte differentiation through a reduction of C/EBPbeta activity and perilipin gene expression. Mol. Endocrinol., 23, 759-771 (2009). PMID 19324970.
user report Nakayama Y., Bleomycin-induced over-replication involves sustained inhibition of mitotic entry through the ATM/ATR pathway. Exp. Cell Res., 315, 2515-2528 (2009). PMID 19527713.

2018.03.15

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