Resource data sheet
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pENTR4-H1

Expression vector of RNAi driven by human H1 promoter.

Catalog number RDB04395
Resource name pENTR4-H1
Clone info. Expression vector of RNAi driven by human H1 promoter. Frequently requested along with Gateway(R) destination vectors for constructing short hairpin RNA (shRNA) expressing vectors.
Vector backbone pENTR4 (Plasmid)
Selectable markers Kan^r
Gene/insert name Human RPPH1 genomic DNA
Originator Miyoshi, Hiroyuki |
Lentiviral plasmid list, maps and sequences information. [link]

Distribution information

Terms and conditions for distribution The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe Dr. Hiroyuki Miyoshi as the Developer.
Ordering Please visit Ordering instruction. 
Order form 
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Material Transfer Agreement (MTA) [Word]
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB04395 pENTR4-H1 DNA solution

Ordering instruction of plasmids [in Japanese] [in English]

References and tips

Electronic file

Electronic file Sequence RDB04395z.seq
Electronic file Remarks, protocol and/or map (pdf) RDB04395.pdf

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB08058_A5Bcp1-2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pDONR_R | Sequence: RDB08058_A5Bca.seq
>04395_08058_A5Bc_pDONR.R_H10_22.ab1
    1 GGCAGACGTC AGCTGGATGG CAATAATGAT TTTATTTTGA CTGATAGTGA CCTGTTCGTT
   61 GCAACAAATT GATAAGCAAT GCTTTCTTAT AATGCCAACT TTGTACAAGA AAGCTGGGTC
  121 TAGATATCTC GACTCTAGAG GATCCCCGGC AGATCTGTGG TCTCATACAG AACTTATAAG
  181 ATTCCCAAAT CCAAAGACAT TTCACGTTTA TGGTGATTTC CCAGAACACA TAGCGACATG
  241 CAAATATTGC AGGGCGCCAC TCCCCTGTCC CTCACAGCCA TCTTCCTGCC AGGGCGCACG
  301 CGCGCTGGGT GTTCCCGCCT AGTGACACTG GGCCCGCGAT TCCTTGGAGC GGGTTGATGA
  361 CGTCAGCGTT CGAATTCGGT ACCGGATCCA GTCGACTGAA TTGGTTCCCA TGGTGGAGCC
  421 TGCTTTTTTG TACAAAGTTG GCATTATAAA AAAGCATTGC TTATCAATTT GTTGCAACGA
  481 ACAGGTCACT ATCAGTCAAA ATAAAATCAT TATTTGGGGC CCGAGCTTAA GTAACTAACT
  541 AACAGGAAGA GTTTGTAGAA ACGCAAAAAG GCCATCCGTC AGGATGGCCT TCTGCTTAGT
  601 TTGATGCCTG GCAGTTTATG GCGGGCGTCC TGCCCGCCAC CCTCCGGGCC GTTGCTTCAC
  661 AACGTTCAAA TCCGCTCCCG GCGGATTTGT CCTACTCAGG AGAGCGTTCA CCGACAAACA
  721 ACAGATAAAA CGAAAGGCCC AGTCTTCCGA CTGAGCCTTT CGTTTTATTT GATGCCTGGC
  781 AGTTCCCTAC TCTCGCTTAG TAGTTAGACG TCCCCGAGAT CCATGCTAGC GGTAATACGG
  841 GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAAACAT GTGAGCAAAA GGCCAGCAAA
  901 AGGCCAGGAA CCGTAAAATG GCCGCGTTTG CTGGCGTTTT TCATAGGCTC CCGCCCCCCT
  961 GACGAGCAAT CACAAAAATC GGACGCCTCA AGGTCAGAAG GTGGCGAAGC CGACAGAACC
1021 TATAAAGATC AGCGTTCCCT GAGCTTCC
//
Primer: KanR-N_R | Sequence: RDB08058_A5Bcb.seq
>04395_08058_A9FpF_KanR_H06_24_ABI24.ab1
    1 GCCATTTATA CCCATATAAA TCAGCATCCA TGTTGGAATT TAATCGCGGC CTCGACGTTT
   61 CCCGTTGAAT ATGGCTCATA ACACCCCTTG TATTACTGTT TATGTAAGCA GACAGTTTTA
  121 TTGTTCATGA TGATATATTT TTATCTTGTG CAATGTAACA TCAGAGATTT TGAGACACGG
  181 GCCAGAGCTG CAGCTGGATG GCAAATAATG ATTTTATTTT GACTGATAGT GACCTGTTCG
  241 TTGCAACAAA TTGATAAGCA ATGCTTTCTT ATAATGCCAA CTTTGTACAA GAAAGCTGGG
  301 TCTAGATATC TCGACTCTAG AGGATCCCCG GCAGATCTGT GGTCTCATAC AGAACTTATA
  361 AGATTCCCAA ATCCAAAGAC ATTTCACGTT TATGGTGATT TCCCAGAACA CATAGCGACA
  421 TGCAAATATT GCAGGGCGCC ACTCCCCTGT CCCTCACAGC CATCTTCCTG CCAGGGCGCA
  481 CGCGCGCTGG GTGTTCCCGC CTAGTGACAC TGGGCCCGCG ATTCCTTGGA GCGGGTTGAT
  541 GACGTCAGCG TTCGAATTCG GTACCGGATC CAGTCGACTG AATTGGTTCC CATGGTGGAG
  601 CCTGCTTTTT TGTACAAAGT TGGCATTATA AAAAAGCATT GCTTATCAAT TTGTTGCAAC
  661 GAACAGGTCA CTATCAGTCA AAATAAAATC ATTATTTGGG GCCCGAGCTT AAGTAACTAA
  721 CTAACAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA
  781 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC
  841 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA
  901 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG
  961 GCAGTTCCCT ACTCTCGCTT AGTAGTTAGA CGTCCCCGAG ATCCATGCTA GCGGGTAAAT
 1021 ACGGTTATCC CACAGAATCA GGGGATAACG CAGGAAAGGA CATGTGAGCA AAAAGGCCAG
1081 CAAAAGCAGG CACCGTAAAA GCGCGATTGC TGCGATATAC GATAGACTTC CC
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

user report Yuki, R., Desuppression of TGF-beta signaling via nuclear c-Abl-mediated phosphorylation of TIF1 gamma/TRIM33 at Tyr-524, -610, and -1048. Oncogene 38 (5): 637-655 (2019). PMID 30177833.
user report Ito, T., Mathematical analysis of gefitinib resistance of lung adenocarcinoma caused by MET amplification. Biochem. Biophys. Res. Commun. 511 (3): 544-550 (2019). PMID 30824185.
user report Nakajo, H., Role for tyrosine phosphorylation of SUV39H1 histone methyltransferase in enhanced trimethylation of histone H3K9 via neuregulin-1/ErbB4 nuclear signaling. Biochem. Biophys. Res. Commun. 511 (4): 765-771 (2019). PMID 30833073.
user report Kamil, M., High filamin-C expression predicts enhanced invasiveness and poor outcome in glioblastoma multiforme. Br. J. Cancer. 120 (8): 819-826 (2019). PMID 30867563.
user report Makino, Y., Single cell RNA-sequencing identified Dec2 as a suppressive factor for spermatogonial differentiation by inhibiting Sohlh1 expression. Sci. Rep. 9 (1): 6063 (2019). PMID 30988352.
user report Jinnou, H., Radial Glial Fibers Promote Neuronal Migration and Functional Recovery after Neonatal Brain Injury. Cell Stem Cell 22 (1): 128-137.e9. (2018). PMID 29276142.
user report Kawasaki, N., TUFT1 interacts with RABGAP1 and regulates mTORC1 signaling Cell Discovery 4 (1): (2018). PMID 29423269.
user report Funato, K., SIRT2-mediated inactivation of p73 is required for glioblastoma tumorigenicity. EMBO Rep. 19 (11). pii: e45587 (2018). PMID 30213795.
user report Neri, S., Fibroblast-led cancer cell invasion is activated by epithelial-mesenchymal transition through platelet-derived growth factor BB secretion of lung adenocarcinoma. Cancer Lett. 395: 20-30 (2017). PMID 28286261.
user report Ishibashi, M., CD200-positive cancer associated fibroblasts augment the sensitivity of Epidermal Growth Factor Receptor mutation-positive lung adenocarcinomas to EGFR Tyrosine kinase inhibitors. Sci. Rep. 7: 46662 (2017). PMID 28429795.
user report Katsura, A., MicroRNA-31 is a positive modulator of endothelial-mesenchymal transition and associated secretory phenotype induced by TGF-beta. Genes Cells 21 (1): 99-116 PMID 26663584.
user report Hikasa, H., Merlin/NF2-Lin28B-let-7 Is a Tumor-Suppressive Pathway that Is Cell-Density Dependent and Hippo Independent. Cell Rep. 14 (12): 2950-2961 (2016). PMID 26997273.
user report Matsuda, Y., Epigenetic Heterogeneity in HIV-1 Latency Establishment. Sci. Rep. 5: 7701 (2015). PMID 25572573.
user report Neri, S., Podoplanin-expressing cancer-associated fibroblasts lead and enhance the local invasion of cancer cells in lung adenocarcinoma. Int. J. Cancer. 137 (4): 784-796 (2015). PMID 25648219.
user report Yamagishi M, Polycomb-Mediated Loss of miR-31 Activates NIK-Dependent NF-κB Pathway in Adult T Cell Leukemia and Other Cancers. Cancer Cell, 21 (1): 121-135 (2012). PMID 22264793.
user report Sasaki, M., Equine major histocompatibility complex class I molecules act as entry receptors that bind to equine herpesvirus-1 glycoprotein D. Genes Cells, 16 (4), 343-357 (2011). PMID 21306483.
user report Aoyama, K., Nuclear c-Abl-mediated tyrosine phosphorylation induces chromatin structural changes through histone modifications that include H4K16 hypoacetylation. Exp. Cell Res., 317 (20): 2874-903 (2011). PMID 22001646.
user report Tanaka, Y., Dual Function of Histone H3 Lysine 36 Methyltransferase ASH1 in Regulation of Hox Gene Expression. PLoS One., 6 (11): e28171 (2011). PMID 22140534.
user report Ohoka, N., The orphan nuclear receptor RORalpha restrains adipocyte differentiation through a reduction of C/EBPbeta activity and perilipin gene expression. Mol. Endocrinol., 23, 759-771 (2009). PMID 19324970.
user report Nakayama Y., Bleomycin-induced over-replication involves sustained inhibition of mitotic entry through the ATM/ATR pathway. Exp. Cell Res., 315, 2515-2528 (2009). PMID 19527713.

2019.11.20

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