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pENTR4-H1 (#RDB04395)

Expression vector of RNAi driven by human H1 promoter.


Drawn by SnapGene® software
Sequence information
(Assembled from experimentally sequenced data)		 GenBank Fla File Format
Publication Unpublished bioresource
Test sheet Data Sheet 
 
Clone info. Expression vector of RNAi driven by human H1 promoter. Frequently requested along with Gateway(R) destination vectors for constructing short hairpin RNA (shRNA) expressing vectors.
Vector backbone pENTR4 (Plasmid)
Selectable markers Kan^r
Growth conditions 37C, LB+Kan
Gene/insert name Human RPPH1 genomic DNA
Depositor|Developer Miyoshi, Hiroyuki |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 本件研究材料は、非営利機関の非営利学術研究に限って提供する。本件研究材料を利用した研究結果等を発表する際は、本件研究材料が故三好浩之博士により開発されたことを明示する。

Catalog # Resource name Shipping form Fee
RDB04395 pENTR4-H1 DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

How to cite this biological resource

Materials & Methods section:

The pENTR4-H1 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB04395).

Reference section:

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

user_report Yamagishi, M., Mechanisms of action and resistance in histone methylation-targeted therapy. Nature 627 (8002): 221-228 (2024). PMID 38383791. [link to RRC of NBRP]
user_report Matsumoto, R., AP-1B regulates interactions of epithelial cells and intraepithelial lymphocytes in the intestine. Cell. Mol. Life Sci. 81 (1): 425 (2024). PMID 39369131. [link to RRC of NBRP]
user_report Yang, S., Neutrophil Extracellular Traps Delay Diabetic Wound Healing by Inducing Endothelial-to-Mesenchymal Transition via the Hippo pathway. Int J Biol Sci. 2023 Jan 1;19(1):347-361 (2023). PMID 36594092. [link to RRC of NBRP]
user_report Araki, H., LSD1 defines the fiber type-selective responsiveness to environmental stress in skeletal muscle. Elife 12: e84618 (2023). PMID 36695573. [link to RRC of NBRP]
user_report Watanabe, H., Astrocytic APOE4 genotype-mediated negative impacts on synaptic architecture in human pluripotent stem cell model. Stem Cell Reports 18 (9): 1854-1869 (2023). PMID 37657448. [link to RRC of NBRP]
user_report Truong, T.T.K., Arl4c is involved in tooth germ development through osteoblastic/ameloblastic differentiation. Biochem. Biophys. Res. Commun. 679: 167-174 (2023). PMID 37703759. [link to RRC of NBRP]
user_report Shimizu, K., Diurnal variation in declarative memory and the involvement of SCOP in cognitive functions in nonhuman primates. 02 November 2022, PREPRINT (Version 1) available at Research Square https://doi.org/10.21203/rs.3.rs-2013762/v1 [link to Journal]
user_report Miyakuni, K., Genome-wide analysis of DNA methylation identifies the apoptosis-related gene UQCRH as a tumor suppressor in renal cancer. Mol. Oncol. 16 (3): 732-749 (2022). PMID 34133843. [link to RRC of NBRP]
user_report Yamashita, A., Culture substrate-associated YAP inactivation underlies chondrogenic differentiation of human induced pluripotent stem cells. Stem Cells Transl. Med. 10 (1): 115-127 (2021). PMID 32822104. [link to RRC of NBRP]
user_report Funaki, T., CADM1 promotes malignant features of small-cell lung cancer by recruiting 4.1R to the plasma membrane. Biochem. Biophys. Res. Commun. 534: 172-178 (2021). PMID 33298314. [link to RRC of NBRP]
user_report Totani, H., Autocrine HGF/c-Met signaling pathway confers aggressiveness in lymph node adult T-cell leukemia/lymphoma. Oncogene 39 (35): 5782-5794 (2020). PMID 32747750. [link to RRC of NBRP]
user_report Yuki, R., Desuppression of TGF-beta signaling via nuclear c-Abl-mediated phosphorylation of TIF1 gamma/TRIM33 at Tyr-524, -610, and -1048. Oncogene 38 (5): 637-655 (2019). PMID 30177833. [link to RRC of NBRP]
user_report Ito, T., Mathematical analysis of gefitinib resistance of lung adenocarcinoma caused by MET amplification. Biochem. Biophys. Res. Commun. 511 (3): 544-550 (2019). PMID 30824185. [link to RRC of NBRP]
user_report Nakajo, H., Role for tyrosine phosphorylation of SUV39H1 histone methyltransferase in enhanced trimethylation of histone H3K9 via neuregulin-1/ErbB4 nuclear signaling. Biochem. Biophys. Res. Commun. 511 (4): 765-771 (2019). PMID 30833073. [link to RRC of NBRP]
user_report Kamil, M., High filamin-C expression predicts enhanced invasiveness and poor outcome in glioblastoma multiforme. Br. J. Cancer. 120 (8): 819-826 (2019). PMID 30867563. [link to RRC of NBRP]
user_report Makino, Y., Single cell RNA-sequencing identified Dec2 as a suppressive factor for spermatogonial differentiation by inhibiting Sohlh1 expression. Sci. Rep. 9 (1): 6063 (2019). PMID 30988352. [link to RRC of NBRP]
user_report Yamagishi, M., Targeting Excessive EZH1 and EZH2 Activities for Abnormal Histone Methylation and Transcription Network in Malignant Lymphomas. Cell Rep. 29 (8): 2321-2337.e7 (2019). PMID 31747604. [link to RRC of NBRP]
user_report Jinnou, H., Radial Glial Fibers Promote Neuronal Migration and Functional Recovery after Neonatal Brain Injury. Cell Stem Cell 22 (1): 128-137.e9. (2018). PMID 29276142. [link to RRC of NBRP]
user_report Kawasaki, N., TUFT1 interacts with RABGAP1 and regulates mTORC1 signaling Cell Discovery 4 (1): (2018). PMID 29423269. [link to RRC of NBRP]
user_report Funato, K., SIRT2-mediated inactivation of p73 is required for glioblastoma tumorigenicity. EMBO Rep. 19 (11). pii: e45587 (2018). PMID 30213795. [link to RRC of NBRP]
user_report Neri, S., Fibroblast-led cancer cell invasion is activated by epithelial-mesenchymal transition through platelet-derived growth factor BB secretion of lung adenocarcinoma. Cancer Lett. 395: 20-30 (2017). PMID 28286261. [link to RRC of NBRP]
user_report Ishibashi, M., CD200-positive cancer associated fibroblasts augment the sensitivity of Epidermal Growth Factor Receptor mutation-positive lung adenocarcinomas to EGFR Tyrosine kinase inhibitors. Sci. Rep. 7: 46662 (2017). PMID 28429795. [link to RRC of NBRP]
user_report Katsura, A., MicroRNA-31 is a positive modulator of endothelial-mesenchymal transition and associated secretory phenotype induced by TGF-beta. Genes Cells 21 (1): 99-116 PMID 26663584. [link to RRC of NBRP]
user_report Takahashi, Y., Perilipin2 plays a positive role in adipocytes during lipolysis by escaping proteasomal degradation. Sci Rep. 6: 20975 (2016). PMID 26876687. [link to RRC of NBRP]
user_report Hikasa, H., Merlin/NF2-Lin28B-let-7 Is a Tumor-Suppressive Pathway that Is Cell-Density Dependent and Hippo Independent. Cell Rep. 14 (12): 2950-2961 (2016). PMID 26997273. [link to RRC of NBRP]
user_report Johmura, Y., Defective DNA repair increases susceptibility to senescence through extension of Chk1-mediated G2 checkpoint activation. Sci. Rep. 6: 31194 (2016). PMID 27507734. [link to RRC of NBRP]
user_report Inoue, J., Identification of BCL11B as a regulator of adipogenesis. Sci. Rep. 6: 32750 (2016). PMID 27586877. [link to RRC of NBRP]
user_report Matsuda, Y., Epigenetic Heterogeneity in HIV-1 Latency Establishment. Sci. Rep. 5: 7701 (2015). PMID 25572573. [link to RRC of NBRP]
user_report Neri, S., Podoplanin-expressing cancer-associated fibroblasts lead and enhance the local invasion of cancer cells in lung adenocarcinoma. Int. J. Cancer. 137 (4): 784-796 (2015). PMID 25648219. [link to RRC of NBRP]
user_report Yamagishi M, Polycomb-Mediated Loss of miR-31 Activates NIK-Dependent NF-κB Pathway in Adult T Cell Leukemia and Other Cancers. Cancer Cell, 21 (1): 121-135 (2012). PMID 22264793. [link to RRC of NBRP]
user_report Sasaki, M., Equine major histocompatibility complex class I molecules act as entry receptors that bind to equine herpesvirus-1 glycoprotein D. Genes Cells, 16 (4), 343-357 (2011). PMID 21306483. [link to RRC of NBRP]
user_report Aoyama, K., Nuclear c-Abl-mediated tyrosine phosphorylation induces chromatin structural changes through histone modifications that include H4K16 hypoacetylation. Exp. Cell Res., 317 (20): 2874-903 (2011). PMID 22001646. [link to RRC of NBRP]
user_report Tanaka, Y., Dual Function of Histone H3 Lysine 36 Methyltransferase ASH1 in Regulation of Hox Gene Expression. PLoS One., 6 (11): e28171 (2011). PMID 22140534. [link to RRC of NBRP]
user_report Ohoka, N., The orphan nuclear receptor RORalpha restrains adipocyte differentiation through a reduction of C/EBPbeta activity and perilipin gene expression. Mol. Endocrinol., 23, 759-771 (2009). PMID 19324970. [link to RRC of NBRP]
user_report Nakayama Y., Bleomycin-induced over-replication involves sustained inhibition of mitotic entry through the ATM/ATR pathway. Exp. Cell Res., 315, 2515-2528 (2009). PMID 19527713. [link to RRC of NBRP]
user_report Tanabe, R., PRRX1 induced by BMP signaling decreases tumorigenesis by epigenetically regulating glioma-initiating cell properties via DNA methyltransferase 3A. Mol. Oncol. 16 (1): 269-288 (2022). PMID 34214250. [link to RRC of NBRP]