pENTR4-H1 (#RDB04395)

Expression vector of RNAi driven by human H1 promoter.

Drawn by SnapGene® software

Sequence information (Assembled from experimentally sequenced data) GenBank Flat File Format open SnapGene file download Publication Unpublished bioresource Test sheet Data Sheet open

 

Clone info.	Expression vector of RNAi driven by human H1 promoter. Frequently requested along with Gateway(R) destination vectors for constructing short hairpin RNA (shRNA) expressing vectors.
Vector backbone	pENTR4 (Plasmid)
Selectable markers	Kan^r
Growth conditions	37C, LB+Kan
Gene/insert name	Human RPPH1 genomic DNA
Depositor|Developer	Miyoshi, Hiroyuki |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link]
Terms and conditions for distribution	The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer.

提供案内 (日本国内) [open/close]

Catalog #	Resource name	Shipping form	Fee
RDB04395	pENTR4-H1	DNA solution

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How to cite this biological resource

Materials & Methods section:

The pENTR4-H1 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB04395).

Reference section:

Further references such as user reports and related articles (go to bottom)

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References

Original, user report and related articles

user_report	Yamagishi, M., Mechanisms of action and resistance in histone methylation-targeted therapy. Nature 627 (8002): 221-228 (2024). PMID 38383791. [PubMed] [Article] [RRC of NBRP]
user_report	Matsumoto, R., AP-1B regulates interactions of epithelial cells and intraepithelial lymphocytes in the intestine. Cell. Mol. Life Sci. 81 (1): 425 (2024). PMID 39369131. [PubMed] [Article] [RRC of NBRP]
user_report	Yang, S., Neutrophil Extracellular Traps Delay Diabetic Wound Healing by Inducing Endothelial-to-Mesenchymal Transition via the Hippo pathway. Int J Biol Sci. 2023 Jan 1;19(1):347-361 (2023). PMID 36594092. [PubMed] [Article] [RRC of NBRP]
user_report	Araki, H., LSD1 defines the fiber type-selective responsiveness to environmental stress in skeletal muscle. Elife 12: e84618 (2023). PMID 36695573. [PubMed] [Article] [RRC of NBRP]
user_report	Watanabe, H., Astrocytic APOE4 genotype-mediated negative impacts on synaptic architecture in human pluripotent stem cell model. Stem Cell Reports 18 (9): 1854-1869 (2023). PMID 37657448. [PubMed] [Article] [RRC of NBRP]
user_report	Truong, T.T.K., Arl4c is involved in tooth germ development through osteoblastic/ameloblastic differentiation. Biochem. Biophys. Res. Commun. 679: 167-174 (2023). PMID 37703759. [PubMed] [Article] [RRC of NBRP]
user_report	Shimizu, K., Diurnal variation in declarative memory and the involvement of SCOP in cognitive functions in nonhuman primates. 02 November 2022, PREPRINT (Version 1) available at Research Square https://doi.org/10.21203/rs.3.rs-2013762/v1 [Article]
user_report	Miyakuni, K., Genome-wide analysis of DNA methylation identifies the apoptosis-related gene UQCRH as a tumor suppressor in renal cancer. Mol. Oncol. 16 (3): 732-749 (2022). PMID 34133843. [PubMed] [Article] [RRC of NBRP]
user_report	Yamashita, A., Culture substrate-associated YAP inactivation underlies chondrogenic differentiation of human induced pluripotent stem cells. Stem Cells Transl. Med. 10 (1): 115-127 (2021). PMID 32822104. [PubMed] [Article] [RRC of NBRP]
user_report	Funaki, T., CADM1 promotes malignant features of small-cell lung cancer by recruiting 4.1R to the plasma membrane. Biochem. Biophys. Res. Commun. 534: 172-178 (2021). PMID 33298314. [PubMed] [Article] [RRC of NBRP]
user_report	Totani, H., Autocrine HGF/c-Met signaling pathway confers aggressiveness in lymph node adult T-cell leukemia/lymphoma. Oncogene 39 (35): 5782-5794 (2020). PMID 32747750. [PubMed] [Article] [RRC of NBRP]
user_report	Yuki, R., Desuppression of TGF-beta signaling via nuclear c-Abl-mediated phosphorylation of TIF1 gamma/TRIM33 at Tyr-524, -610, and -1048. Oncogene 38 (5): 637-655 (2019). PMID 30177833. [PubMed] [Article] [RRC of NBRP]
user_report	Ito, T., Mathematical analysis of gefitinib resistance of lung adenocarcinoma caused by MET amplification. Biochem. Biophys. Res. Commun. 511 (3): 544-550 (2019). PMID 30824185. [PubMed] [Article] [RRC of NBRP]
user_report	Nakajo, H., Role for tyrosine phosphorylation of SUV39H1 histone methyltransferase in enhanced trimethylation of histone H3K9 via neuregulin-1/ErbB4 nuclear signaling. Biochem. Biophys. Res. Commun. 511 (4): 765-771 (2019). PMID 30833073. [PubMed] [Article] [RRC of NBRP]
user_report	Kamil, M., High filamin-C expression predicts enhanced invasiveness and poor outcome in glioblastoma multiforme. Br. J. Cancer. 120 (8): 819-826 (2019). PMID 30867563. [PubMed] [Article] [RRC of NBRP]
user_report	Makino, Y., Single cell RNA-sequencing identified Dec2 as a suppressive factor for spermatogonial differentiation by inhibiting Sohlh1 expression. Sci. Rep. 9 (1): 6063 (2019). PMID 30988352. [PubMed] [Article] [RRC of NBRP]
user_report	Yamagishi, M., Targeting Excessive EZH1 and EZH2 Activities for Abnormal Histone Methylation and Transcription Network in Malignant Lymphomas. Cell Rep. 29 (8): 2321-2337.e7 (2019). PMID 31747604. [PubMed] [Article] [RRC of NBRP]
user_report	Jinnou, H., Radial Glial Fibers Promote Neuronal Migration and Functional Recovery after Neonatal Brain Injury. Cell Stem Cell 22 (1): 128-137.e9. (2018). PMID 29276142. [PubMed] [Article] [RRC of NBRP]
user_report	Kawasaki, N., TUFT1 interacts with RABGAP1 and regulates mTORC1 signaling Cell Discovery 4 (1): (2018). PMID 29423269. [PubMed] [Article] [RRC of NBRP]
user_report	Funato, K., SIRT2-mediated inactivation of p73 is required for glioblastoma tumorigenicity. EMBO Rep. 19 (11). pii: e45587 (2018). PMID 30213795. [PubMed] [Article] [RRC of NBRP]
user_report	Neri, S., Fibroblast-led cancer cell invasion is activated by epithelial-mesenchymal transition through platelet-derived growth factor BB secretion of lung adenocarcinoma. Cancer Lett. 395: 20-30 (2017). PMID 28286261. [PubMed] [Article] [RRC of NBRP]
user_report	Ishibashi, M., CD200-positive cancer associated fibroblasts augment the sensitivity of Epidermal Growth Factor Receptor mutation-positive lung adenocarcinomas to EGFR Tyrosine kinase inhibitors. Sci. Rep. 7: 46662 (2017). PMID 28429795. [PubMed] [Article] [RRC of NBRP]
user_report	Katsura, A., MicroRNA-31 is a positive modulator of endothelial-mesenchymal transition and associated secretory phenotype induced by TGF-beta. Genes Cells 21 (1): 99-116 PMID 26663584. [PubMed] [Article] [RRC of NBRP]
user_report	Takahashi, Y., Perilipin2 plays a positive role in adipocytes during lipolysis by escaping proteasomal degradation. Sci Rep. 6: 20975 (2016). PMID 26876687. [PubMed] [Article] [RRC of NBRP]
user_report	Hikasa, H., Merlin/NF2-Lin28B-let-7 Is a Tumor-Suppressive Pathway that Is Cell-Density Dependent and Hippo Independent. Cell Rep. 14 (12): 2950-2961 (2016). PMID 26997273. [PubMed] [Article] [RRC of NBRP]
user_report	Johmura, Y., Defective DNA repair increases susceptibility to senescence through extension of Chk1-mediated G2 checkpoint activation. Sci. Rep. 6: 31194 (2016). PMID 27507734. [PubMed] [Article] [RRC of NBRP]
user_report	Inoue, J., Identification of BCL11B as a regulator of adipogenesis. Sci. Rep. 6: 32750 (2016). PMID 27586877. [PubMed] [Article] [RRC of NBRP]
user_report	Matsuda, Y., Epigenetic Heterogeneity in HIV-1 Latency Establishment. Sci. Rep. 5: 7701 (2015). PMID 25572573. [PubMed] [Article] [RRC of NBRP]
user_report	Neri, S., Podoplanin-expressing cancer-associated fibroblasts lead and enhance the local invasion of cancer cells in lung adenocarcinoma. Int. J. Cancer. 137 (4): 784-796 (2015). PMID 25648219. [PubMed] [Article] [RRC of NBRP]
user_report	Yamagishi M, Polycomb-Mediated Loss of miR-31 Activates NIK-Dependent NF-κB Pathway in Adult T Cell Leukemia and Other Cancers. Cancer Cell, 21 (1): 121-135 (2012). PMID 22264793. [PubMed] [Article] [RRC of NBRP]
user_report	Sasaki, M., Equine major histocompatibility complex class I molecules act as entry receptors that bind to equine herpesvirus-1 glycoprotein D. Genes Cells, 16 (4), 343-357 (2011). PMID 21306483. [PubMed] [Article] [RRC of NBRP]
user_report	Aoyama, K., Nuclear c-Abl-mediated tyrosine phosphorylation induces chromatin structural changes through histone modifications that include H4K16 hypoacetylation. Exp. Cell Res., 317 (20): 2874-903 (2011). PMID 22001646. [PubMed] [Article] [RRC of NBRP]
user_report	Tanaka, Y., Dual Function of Histone H3 Lysine 36 Methyltransferase ASH1 in Regulation of Hox Gene Expression. PLoS One., 6 (11): e28171 (2011). PMID 22140534. [PubMed] [Article] [RRC of NBRP]
user_report	Ohoka, N., The orphan nuclear receptor RORalpha restrains adipocyte differentiation through a reduction of C/EBPbeta activity and perilipin gene expression. Mol. Endocrinol., 23, 759-771 (2009). PMID 19324970. [PubMed] [Article] [RRC of NBRP]
user_report	Nakayama Y., Bleomycin-induced over-replication involves sustained inhibition of mitotic entry through the ATM/ATR pathway. Exp. Cell Res., 315, 2515-2528 (2009). PMID 19527713. [PubMed] [Article] [RRC of NBRP]
user_report	Tanabe, R., PRRX1 induced by BMP signaling decreases tumorigenesis by epigenetically regulating glioma-initiating cell properties via DNA methyltransferase 3A. Mol. Oncol. 16 (1): 269-288 (2022). PMID 34214250. [PubMed] [Article] [RRC of NBRP]

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External Database
Human RPPH1

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Reference sequence
  

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