Resource data sheet
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pCMV-VSV-G-RSV-Rev

Expression vector of emvelope VSV G protein and Rev for lentivirus vector packaging.

Catalog number RDB04393
Resource name pCMV-VSV-G-RSV-Rev
Clone info. Expression vector of emvelope VSV G protein and Rev for producing lentiviral particles. Use in conjunction with a packaging vector such as pCAG-HIVgp (RDB04394).
Commonly requested with pCAG-HIVgp (RDB04394).
Vector backbone Plasmid
Selectable markers Amp^r
Originator Miyoshi, Hiroyuki |

Distribution information

CLICK here for plasmid list and map. [link]
Forms for plasmid request [in Japanese] [in English]
Terms and conditions for distribution The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose.
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Remarks ((Remarks)) Remember that you will be working with samples containing infectious virus.
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Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB04393_A5Aqp1_2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward RDB04393_A5Aqa.seq
>04393_04393_A5Aq_CMV-Forward_A04_01.ab1
    1 CTGTCACTCC GCCCCATTGA CGCAATGGGC GGTAGGCGTG TACGGTGGGA GGTCTATATA
   61 AGCAGAGCTC TCTGGCTAAC TAGAGAACCC ACTGCTTACT GGCTTATCGA AATTAATACG
  121 ACTCACTATA GGGAGACCCA AGCTGGCTAG CGTTTAAACT TAAGCTTACA TGTGGTACCG
  181 AGCTCGGATC CTGAGAACTT CAGGGTGAGT CTATGGGACC CTTGATGTTT TCTTTCCCCT
  241 TCTTTTCTAT GGTTAAGTTC ATGTCATAGG AAGGGGAGAA GTAACAGGGT ACACATATTG
  301 ACCAAATCAG GGTAATTTTG CATTTGTAAT TTTAAAAAAT GCTTTCTTCT TTTAATATAC
  361 TTTTTTGTTT ATCTTATTTC TAATACTTTC CCTAATCTCT TTCTTTCAGG GCAATAATGA
  421 TACAATGTAT CATGCCTCTT TGCACCATTC TAAAGAATAA CAGTGATAAT TTCTGGGTTA
  481 AGGCAATAGC AATATTTCTG CATATAAATA TTTCTGCATA TAAATTGTAA CTGATGTAAG
  541 AGGTTTCATA TTGCTAATAG CAGCTACAAT CCAGCTACCA TTCTGCTTTT ATTTTATGGT
  601 TGGGATAAGG CTGGATTATT CTGAGTCCAA GCTAGGCCCT TTTGCTAATC ATGTTCATAC
  661 CTCTTATCTT CCTCCCACAG CTCCTGGGCA ACGTGCTGGT CTGTGTGCTG GCCCATCACT
  721 TTGGCAAAGA ATTCTGACAC TATGAAGTGC CTTTTGTACT TAGCCTTTTT ATTCATTGGG
  781 GTGAATTGCA AGTTCACCAT AGTTTTTCCA CACAACCAAA AAGGAAACTG GAAAAATGTT
  841 CCTTCTAATT ACCATTATTG CCCGTCAAGC TCAGATTTAA ATTGGCATAA TGACTTAATA
  901 GGCACAGCCT TACAAGTCAA AATGCCCAAG AGTCACAAAG GCTATTCAAG CAGACGGTTG
  961 GATGTGTCAT GCTTCCAAAT GGGTCACTAC TTTGTGATTT CCCGCTGGTA TGGACCGAAG
 1021 TATATAACAC ATTCCCATCC CGATCCTTTC ACTCCATCTG TAGAACAATG CAAGGAAAGG
 1081 CATTGGACAA CGAAAACAGG GAACTTGGCT GAATCCAGGC CTCCCTCCTC AAGGTTGTGG
1141 CATATGCCAC CTGTGACGGA ATGCGAAGTC ATGCAATTGT TCCAGGGGTT GAAGCT
//
Primer: BGH_rev2 RDB04393_A5Aqb.seq
>04393_04393_A5Aq_BGHrev_B04_04.ab1
    1 CTTTCGGGTC TCTAGACTCG AGCGGCCGCC ACTGTGCTGG ATATCTGCAG AATTCCGTTT
   61 TTTTTTTTTT TTTTCATAAA AATTAAAAAC TCAAATATAA TTGAGGCCTC TTTGAGCATG
  121 GTATCACAAG TTGATTTGGT CCAAACATGA AGAATCTGTT GTGCAGGATT TGAGTTACTT
  181 TCCAAGTCGG TTCATCTCTA TGTCTGTATA AATCTGTCTT TTCTTGGTGT GCTTTAATTT
  241 AATGCAAAGA TGGATACCAA CTCGGAGAAC CAAGAATAGT CCAATGATTA ACCCTATGAT
  301 AAAGAAAAAA GAGGCAATAG AGCTTTTCCA ACTACTGAAC CAACCTTCTA CAAGCTCGAT
  361 TGGATTTTTG GATAGCCCAG TATCACCAAA AAATAAACTC TCATCATCAG GAAGTTGCGA
  421 AGCAGCGTCT TGAATGTGAG GATGTTCGAA CACCTGAGCC TTTGAGCTAA GATGAAGATC
  481 GGAGTCCAAC ATACCATGTC CAATCATGTA TAAAGGAAAC TTATATCCTG AACTGGTCCT
  541 CAGAACTCCA TTGGGTCCAA TTTCCACGTC TTCATATGGT GCCCAGTCAT CCCACAGTTC
  601 CCTTTCTGTG GTAGTTCCAC TGATCATTCC GACCATTCTT GAGAGGATTG GAGCAGCAAT
  661 ATCGACTCTG ATGTATCTGG TCTCAAAGTA TTTTAGGGTA CCATTGATTA TGGTGAAAGC
  721 AGGACCGGTT CCTGGGTTTT TAGGAGCAAG ATAGCTGAGA TCCACTGGAG AGATTGGAAG
  781 ACCCGCTCTG ATTTTGCTCC AGGTTTCTTG GCAGAGGGAA TAATCCAAGA TCCTCTCAAC
  841 GTCCTGAATT AGACTTACAT CCACTGAGGT CTGAGATGGA GCAGAGATAC TTGACCCTTC
  901 TGGGCATTCA GGGAATCTGG CTGCAGCAAA GAGATCCTTA TCAGCCATCT CGAACCAGAC
  961 ACCTGATGGG AGTCTGACTC CCCAATGCTT TGCAGTATTG CATTTTGCAG GCCTTTGCCT
 1021 CCAGGTTTCA TAAGGCAAAG TAGTTACTTC TGAACCTGTG CCCTCCGTTT CCAGGGATGA
 1081 TAGCTTCTTC GTCCTCTGAG AGAGTTGAAT GGTCATGGCA ATGGAGGTTA GAATTCCACA
1141 TTAGCCCTTT GTGC
//
Primer: Reverse2 RDB04393_A5Aqc.seq
>04393_04393_A5Aq_Reverse2_C04_07.ab1
    1 CAGTTAATGA TTCGGAATTC GATGTACGGG CCAGATATAC GCGTATCTGA GGGGACTAGG
   61 GTGTGTTTAG GCGAAAAGCG GGGCTTCGGT TGTACGCGGT TAGGAGTCCC CTCAGGATAT
  121 AGTAGTTTCG CTTTTGCATA GGGAGGGGGA AATGTAGTCT TATGCAATAC TCTTGTAGTC
  181 TTGCAACATG GTAACGATGA GTTAGCAACA TGCCTTACAA GGAGAGAAAA AGCACCGTGC
  241 ATGCCGATTG GTGGAAGTAA GGTGGTACGA TCGTGCCTTA TTAGGAAGGC AACAGACGGG
  301 TCTGACATGG ATTGGACGAA CCACTGAATT CCGCATTGCA GAGATATTGT ATTTAAGTGC
  361 CTAGCTCGAT ACAATAAACG CCATTTGACC ATTCACCACA TTGGTGTGCA CCTCCAAGCT
  421 CGAGCTCGTT TAGTGAACCG TCAGATCGCC TGGAGACGCC ATCCACGCTG TTTTGACCTC
  481 CATAGAAGAC ACCGGGACCG ATCCAGCCTC CCCTCGAAGC TAGTCGATTA GGCATCTCCT
  541 ATGGCAGGAA GAAGCGGAGA CAGCGACGAA GACCTCCTCA AGGCAGTCAG ACTCATCAAG
  601 TTTCTCTATC AAAGCAACCC ACCTCCCAAT CCCGAGGGGA CCCGACAGGC CCGAAGGAAT
  661 AGAAGAAGAA GGTGGAGAGA GAGACAGAGA CAGATCCATT CGATTAGTGA ACGGATCCTT
  721 AGCACTTATC TGGGACGATC TGCGGAGCCT GTGCCTCTTC AGCTACCACC GCTTGAGAGA
  781 CTTACTCTTG ATTGTAACGA GGATTGTGGA ACTTCTGGGA CGCAGGGGGT GGGAAGCCCT
  841 CAAATATTGG TGGAATCTCC TACAATATTG GAGTCAGGAG CTAAAGAATA GTGCTGTTAG
  901 CTTGCTCAAT GCCACAGCTA TAGCAGTAGC TGAGGGGACA GATAGGGGTT ATAGAAGTAG
  961 TACAAGAAGC TTGGGCACTG GCCGTCGTTT TACAACGTCG TGATCTGAGC CTGGGAGATC
 1021 TCTGGCTAAC TAGGGAAACC CACTGCTTAA GCCCTCAATA AAGCTTGCCC TTGAGTGCTT
 1081 CAAGTAGTGT GTGCCCGTCC TGTTGTGGTG ACTCTGGTTA CCTAGAAGAT CAAGGAAAAC
1141 CCCTTGGGCG GTTTTACCCA AACCATA
//
Primer: RSVpro_F RDB04393_A5Aqd.seq
>04393_04393_A5Aq_RSVpro_F_B01_02_ABI08.ab1
    1 AGCCCATTTT GGACCATTCA CCACATTGGT GTGCACCTCC AAGCTCGAGC TCGTTTAGTG
   61 AACCGTCAGA TCGCCTGGAG ACGCCATCCA CGCTGTTTTG ACCTCCATAG AAGACACCGG
  121 GACCGATCCA GCCTCCCCTC GAAGCTAGTC GATTAGGCAT CTCCTATGGC AGGAAGAAGC
  181 GGAGACAGCG ACGAAGACCT CCTCAAGGCA GTCAGACTCA TCAAGTTTCT CTATCAAAGC
  241 AACCCACCTC CCAATCCCGA GGGGACCCGA CAGGCCCGAA GGAATAGAAG AAGAAGGTGG
  301 AGAGAGAGAC AGAGACAGAT CCATTCGATT AGTGAACGGA TCCTTAGCAC TTATCTGGGA
  361 CGATCTGCGG AGCCTGTGCC TCTTCAGCTA CCACCGCTTG AGAGACTTAC TCTTGATTGT
  421 AACGAGGATT GTGGAACTTC TGGGACGCAG GGGGTGGGAA GCCCTCAAAT ATTGGTGGAA
  481 TCTCCTACAA TATTGGAGTC AGGAGCTAAA GAATAGTGCT GTTAGCTTGC TCAATGCCAC
  541 AGCTATAGCA GTAGCTGAGG GGACAGATAG GGTTATAGAA GTAGTACAAG AAGCTTGGCA
  601 CTGGCCGTCG TTTTACAACG TCGTGATCTG AGCCTGGGAG ATCTCTGGCT AACTAGGGAA
  661 CCCACTGCTT AAGCCTCAAT AAAGCTTGCC TTGAGTGCTT CAAGTAGTGT GTGCCCGTCT
  721 GTTGTGTGAC TCTGGTAACT AGAGATCAGG AAAACCCTGG CGTTACCCAA CTTAATCGCC
  781 TTGCAGCACA TCCCCCTTTC GCCAGCTGCA TTAATGAATC GGCCAACGCG CGGGGAGAGG
  841 CGGTTTGCGT ATTGGGCGCT CTTCCGCTTC CTCGCTCACT GACTCGCTGC GCTCGGTCGT
  901 TCGGCTGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC
  961 AGGGGATAAC GCAGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA
 1021 AAGCCCGCGT TGCTGGCGTT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAT
 1081 CGACGCTCAA GTCAGAGTGG CGAACCGACA GACTATAAGA ATACCAGCGT TTCCCCTGAG
 1141 CTTCCCTCGT TGCGCTCTCT GATCCGATCT TACCGCTACC GATACCTGTT CCGCCCTATC
//

Please visit plasmidSequencing and PCR primers for primer information.

References and tips

Electronic file

References

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