RDB04392 - pCMV-VSV-G

Resource data sheet
Prev. | Lentiviral vector plasmid >
Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

pCMV-VSV-G

Expression vector of VSV G protein

Catalog number	RDB04392
Resource name	pCMV-VSV-G
Clone info.	Expression vector of VSV G protein, envelope protein for producing lentiviral particles. Use in conjunction with packaging vectors.
Vector backbone	Plasmid
Selectable markers	Amp^r
Gene/insert name	VSV G protein
Depositor\|Developer	Miyoshi, Hiroyuki \|

Other clones in our bank

VSV G protein and ortholog

External Database
VSV G protein

KEGG Orthology

Reference sequence

Lentiviral plasmid list. [link]

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR The exportation of this material to foreign countries is controlled and restricted by the law of Japan. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer.

Ordering Please visit Ordering instruction.[link]
Order form [Credit Card Payment] [Bank Transfer Payment]
Material Transfer Agreement (MTA, for use for not-for-profit academic purpose)[Word]

Remarks Currently unavailable outside Japan. Remember that you will be working with samples containing infectious virus.

提供案内 (日本国内) [open/close]

Terms and conditions set forth by the DEPOSITOR	The exportation of this material to foreign countries is controlled and restricted by the law of Japan. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer.
Ordering	Please visit Ordering instruction.[link] Order form [Credit Card Payment] [Bank Transfer Payment] Material Transfer Agreement (MTA, for use for not-for-profit academic purpose)[Word]
Remarks	Currently unavailable outside Japan. Remember that you will be working with samples containing infectious virus.

提供条件	本件研究材料は、非営利機関の非営利学術研究に限って提供する。本件研究材料を利用した研究結果等を発表する際は、本件研究材料が故三好浩之博士により開発されたことを明示する。本件リソースの輸出は法令等により規制されている。
提供依頼	手続きの詳細は、「レンチウイルスベクターの提供申し込み」をご覧ください。提供依頼書 [Word] 提供同意書 (非営利機関による非営利学術研究用)[Word] 遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。
備考	このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB04392	pCMV-VSV-G	DNA solution

Ordering instruction of plasmids [in Japanese] [in English]
Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

References and tips

Electronic file

Sequence	RDB04392z.seq
Remarks, protocol and/or map (pdf)	RDB04392.pdf

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content	Lentivirus Vector (English text)
Featured content	Lentivirus Vector (Japanese text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB13313_A8Hfp1-2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward Region: CMV pro,human beta-globin IVS Sequence file: RDB13313_A8Hfc.seq
>04392_13313_A8Hf_CMV_Forward_C01_03_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNNN NNGGGCGGTA NGCGTGTACG GTGGGNGGTC TATATAAGCA 61 GAGCTCTCTG GCTAACTAGA GAACCCACTG CTTACTGGCT TATCGAAATT AATACGACTC 121 ACTATAGGGA GACCCAAGCT GGCTAGCGTT TAAACTTAAG CTTACATGTG GTACCGAGCT 181 CGGATCCTGA GAACTTCAGG GTGAGTCTAT GGGACCCTTG ATGTTTTCTT TCCCCTTCTT 241 TTCTATGGTT AAGTTCATGT CATAGGAAGG GGAGAAGTAA CAGGGTACAC ATATTGACCA 301 AATCAGGGTA ATTTTGCATT TGTAATTTTA AAAAATGCTT TCTTCTTTTA ATATACTTTT 361 TTGTTTATCT TATTTCTAAT ACTTTCCCTA ATCTCTTTCT TTCAGGGCAA TAATGATACA 421 ATGTATCATG CCTCTTTGCA CCATTCTAAA GAATAACAGT GATAATTTCT GGGTTAAGGC 481 AATAGCAATA TTTCTGCATA TAAATATTTC TGCATATAAA TTGTAACTGA TGTAAGAGGT 541 TTCATATTGC TAATAGCAGC TACAATCNAG CTACCATTCT GCTTTTATTT TATGGTTGGG 601 ATAANGCTGG ANTATTCTGA NTCCAAGCTA GGCCCTTTTG CTAATCATGT TCNTACCTCT 661 TATCTTCNTC CNACAGCTCC TGGNCNACGT NCTGGNNNGN NNGCTNGNCC NNTCANNTNN 721 NNNANNNNNT NNNNNNNNNN //
Primer: T7_20bp Region: human beta-globin IVS,insert 5' Sequence file: RDB13313_A8Hfd.seq
>04392_13313_A8Hf_T7_20bp_A05_02_ABI24.ab1 1 CGTGCCAGCG TTTTACTTAA GCTTACATGT GGTACCGAGC TCGGATCCTG AGAACTTCAG 61 GGTGAGTCTA TGGGACCCTT GATGTTTTCT TTCCCCTTCT TTTCTATGGT TAAGTTCATG 121 TCATAGGAAG GGGAGAAGTA ACAGGGTACA CATATTGACC AAATCAGGGT AATTTTGCAT 181 TTGTAATTTT AAAAAATGCT TTCTTCTTTT AATATACTTT TTTGTTTATC TTATTTCTAA 241 TACTTTCCCT AATCTCTTTC TTTCAGGGCA ATAATGATAC AATGTATCAT GCCTCTTTGC 301 ACCATTCTAA AGAATAACAG TGATAATTTC TGGGTTAAGG CAATAGCAAT ATTTCTGCAT 361 ATAAATATTT CTGCATATAA ATTGTAACTG ATGTAAGAGG TTTCATATTG CTAATAGCAG 421 CTACAATCCA GCTACCATTC TGCTTTTATT TTATGGTTGG GATAAGGCTG GATTATTCTG 481 AGTCCAAGCT AGGCCCTTTT GCTAATCATG TTCATACCTC TTATCTTCCT CCCACAGCTC 541 CTGGGCAACG TGCTGGTCTG TGTGCTGGCC CATCACTTTG GCAAAGAATT CTGACACTAT 601 GAAGTGCCTT TTGTACTTAG CCTTTTTATT CATTGGGGTG AATTGCAAGT TCACCATAGT 661 TTTTCCACAC AACCAAAAAG GAAACTGGAA AAATGTTCCT TCTAATTACC ATTATTGCCC 721 GTCAAGCTCA GATTTAAATT GGCATAATGA CTTAATAGGC ACAGCCTTAC AAGTCAAAAT 781 GCCCAAGAGT CACAAGGCTA TTCAAGCAGA CGGTTGGATG TGTCATGCTT CCAAATGGGT 841 CACTACTTGT GATTTCCGCT GGTATGGACC GAAGTATATA ACACATTCCA TCCGATCCTT 901 CACTCCATCT GTAGAACAAT GCAAGGAAAG CATTGAACAA ACGAAACAAG GAAACTTGGC 961 TGAATCCAGG CTTCCCTCCT CAAAGTTGTG GATATGCAAC TGTGACGGAT GCCGAAGCAG 1021 TGATTGTCCA GGTGACTCCC TCACCAATGT GCTGGTTGAA TGAATACACA AGGAGAATGG 1081 GTTGATTCAC AGTCATCAAC GGGAAATGCA GCATAACATA TGCCCACTGT TCAATAAACT 1141 TCTTACCACC CTGGGGCAAT T //
Primer: BGH_rev Region: insert 3' Sequence file: RDB13313_A8Hfe.seq
>04392_13313_A8Hf_BGH_rev_B05_05_ABI24.ab1 1 CTTACGGGAC TCTAGACTCG AGCGGCCGCC ACTGTGCTGG ATATCTGCAG AATTCCGTTT 61 TTTTTTTTTT TTTTCATAAA AATTAAAAAC TCAAATATAA TTGAGGCCTC TTTGAGCATG 121 GTATCACAAG TTGATTTGGT CCAAACATGA AGAATCTGTT GTGCAGGATT TGAGTTACTT 181 TCCAAGTCGG TTCATCTCTA TGTCTGTATA AATCTGTCTT TTCTTGGTGT GCTTTAATTT 241 AATGCAAAGA TGGATACCAA CTCGGAGAAC CAAGAATAGT CCAATGATTA ACCCTATGAT 301 AAAGAAAAAA GAGGCAATAG AGCTTTTCCA ACTACTGAAC CAACCTTCTA CAAGCTCGAT 361 TGGATTTTTG GATAGCCCAG TATCACCAAA AAATAAACTC TCATCATCAG GAAGTTGCGA 421 AGCAGCGTCT TGAATGTGAG GATGTTCGAA CACCTGAGCC TTTGAGCTAA GATGAAGATC 481 GGAGTCCAAC ATACCATGTC CAATCATGTA TAAAGGAAAC TTATATCCTG AACTGGTCCT 541 CAGAACTCCA TTGGGTCCAA TTTCCACGTC TTCATATGGT GCCCAGTCAT CCCACAGTTC 601 CCTTTCTGTG GTAGTTCCAC TGATCATTCC GACCATTCTT GAGAGGATTG GAGCAGCAAT 661 ATCGACTCTG ATGTATCTGG TCTCAAAGTA TTTTAGGGTA CCATTGATTA TGGTGAAAGC 721 AGGACCGGTT CCTGGGTTTT TAGGAGCAAG ATAGCTGAGA TCCACTGGAG AGATTGGAAG 781 ACCCGCTCTG ATTTTGCTCC AGGTTTCTTG GCAGAGGGAA TAATCCAAGA TCCTCTCAAC 841 GTCCTGAATT AGACTTACAT CCACTGAGGT CTGAGATGGA GCAGAGATAC TTGACCCTTC 901 TGGGCATTCA GGGAATCTGG CTGCAGCAAA GAGATCCTTA TCAGCCATCT CGAACCAGAC 961 ACCTGATGGG AGTCTGACTC CCCAATGCTT GCAGTATTGC ATTTTGCAGC CTTGCCCTCC 1021 AGTTTCATAA GCAAAGTAGT TTACTTCTGA ACCCTGTGCC TCTTTCCCAG GGATGATAGC 1081 TCTCCGTTCT CTGAGAGAGT GATGTCCCAT GAATGAGGTA GATTCACATA TTTATGC //
Primer: bGH_rev3 Region: bGHpA,insert 3' Sequence file: RDB13313_A8Hff.seq
>04392_13313_A8Hf_bGH_rev3_D01_04_ABI08.ab1 1 NNNNNNNNNN NNNNGNNGNN GGGCANCAAC AGATGGCTGG CAACTAGAAG GCACAGTCGA 61 GGCTGATCAG CGGGTTTAAA CGGGCCCTCT AGACTCGAGC GGCCGCCACT GTGCTGGATA 121 TCTGCAGAAT TCCGTTTTTT TTTTTTTTTT TCATAAAAAT TAAAAACTCA AATATAATTG 181 AGGCCTCTTT GAGCATGGTA TCACAAGTTG ATTTGGTCCA AACATGAANA ATCTGTTGTG 241 CAGGATTTGA GTTACTTTCC AAGTCGGTTC ATCTCTATGT CTGTATAAAT CTGTCTTTTC 301 TTGGTGTGCT TTAATTTAAT GCAAAGATGG ATACCAACTC GNANAACCAA NAATAGTCCA 361 ATGATTAACC CTATGATAAA GAAAAAAGAG GCAATAGAGC TTTTCCAACT ACTGAACCAA 421 CCTTCTACAA GCTCNATTGG ATTTTTGGAT AGCCCAGTAT CACCAAAAAA TAAACTCTCA 481 TCATCAGGAA GTTGCGAAGC AGCGTCTTGA ATGTGAGGAT GTTCGAACAC CTGAGCCTTT 541 GAGCTAAGAT GAAGATCGGA GTCCAACATA CCATGTCCAA TCATGTATAA AGGAAACTTA 601 TATCCTGAAC TGGTCCTCAN AACTCNNTTG GGTCCNATTT CCACGTCTTC NTATGGNGCC 661 NAGTCATCCC ACAGTTNCCT TTCNGNGGNA NTNNCNCNNN NNNTTNCNNA NNNTNNNNNN 721 NNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

user report	Maruoka, M., Caspase cleavage releases a nuclear protein fragment that stimulates phospholipid scrambling at the plasma membrane. Mol. Cell 81 (7): 1397-1410.e9 (2021). PMID 33725486.
user report	Ryoden, Y., Functional Expression of the P2X7 ATP Receptor Requires Eros. J. Immunol. 204 (3): 559-568 (2020). PMID 31862710.
user report	Fukuda, T., Combinatorial expression of cell cycle regulators is more suitable for immortalization than oncogenic methods in dermal papilla cells. iScience 24 (1): 101929 (2020). PMID 33437932.
user report	Segawa, K., The CDC50A extracellular domain is required for forming a functional complex with and chaperoning phospholipid flippases to the plasma membrane. J. Biol. Chem. 293 (6): 2172-2182 (2018). PMID 29276178.
user report	Endo, R., TAR DNA-Binding Protein 43 and Disrupted in Schizophrenia 1 Coaggregation Disrupts Dendritic Local Translation and Mental Function in Frontotemporal Lobar Degeneration. Biol. Psychiatry. 84(7): 509-521 (2018). PMID 29752072.
user report	Ueno, N., PU.1 acts as tumor suppressor for myeloma cells through direct transcriptional repression of IRF4. Oncogene 36 (31): 4481-4497 (2017). PMID 28368411.
user report	Tomioka, I., Transgenic Monkey Model of the Polyglutamine Diseases Recapitulating Progressive Neurological Symptoms. eNeuro 4 (2). pii: ENEURO.0250-16 (2017). PMID 28374014.
user report	Ujino, S., Hepatitis C virus utilizes VLDLR as a novel entry pathway. Proc. Natl. Acad. Sci. U.S.A. 113 (1): 188-193 (2016). PMID 26699506.
user report	Shimojo, D., Rapid, efficient, and simple motor neuron differentiation from human pluripotent stem cells. Mol. Brain. 8 (1): 79 (2015). PMID 26626025.
user report	Mizutani, M., Baculovirus-Vectored Multistage Plasmodium vivax Vaccine Induces Both Protective and Transmission-Blocking Immunities against Transgenic Rodent Malaria Parasites. Infect. Immun. 82 (10): 4348-4357 (2014). PMID 25092912.
user report	Sekiya, S., Direct conversion of mouse fibroblasts to hepatocyte-like cells by defined factors. Nature 475 (7356): 390-393 (2011). PMID 21716291.
user report	Tanaka, Y., Dual Function of Histone H3 Lysine 36 Methyltransferase ASH1 in Regulation of Hox Gene Expression. PLoS One., 6 (11): e28171 (2011). PMID 22140534.
user report	Fan, J., Halogenated thymidine analogues restore the expression of silenced genes without demethylation. Cancer Res., 65 (15): 6927-6933 (2005). PMID 16061677.

2022.05.02

GNP_filter3_RDBDEP_html_220427.pl