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pCMV-VSV-G

Expression vector of VSV G protein

Catalog number RDB04392
Resource name pCMV-VSV-G
Clone info. Expression vector of VSV G protein, envelope protein for producing lentiviral particles. Use in conjunction with packaging vectors.
Vector backbone Plasmid
Selectable markers Amp^r
Gene/insert name VSV G protein
Depositor|Developer Miyoshi, Hiroyuki |
Other clones in our bank

External Database
VSV G protein

          Reference sequence
            
          Lentiviral plasmid list. [link]

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR The exportation of this material to foreign countries is controlled and restricted by the law of Japan. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer.
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          Material Transfer Agreement (MTA, for use for not-for-profit academic purpose)[Word]
          Remarks Currently unavailable outside Japan. Remember that you will be working with samples containing infectious virus.
          提供案内 (日本国内) [open/close]

          提供条件 本件研究材料は、非営利機関の非営利学術研究に限って提供する。本件研究材料を利用した研究結果等を発表する際は、本件研究材料が故三好浩之博士により開発されたことを明示する。本件リソースの輸出は法令等により規制されている。
          提供依頼 手続きの詳細は、「レンチウイルスベクターの提供申し込み」をご覧ください。
          提供依頼書 [Word]
          提供同意書 (非営利機関による非営利学術研究用)[Word]
          遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。
          備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB04392 pCMV-VSV-G DNA solution

          Ordering instruction of plasmids [in Japanese] [in English]
          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Sequence RDB04392z.seq
          Remarks, protocol and/or map (pdf) RDB04392.pdf

          Original reference

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content Lentivirus Vector (English text)
          Featured content Lentivirus Vector (Japanese text)

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB13313_A8Hfp1-2.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: CMV_Forward
          Region: CMV pro,human beta-globin IVS
          Sequence file: RDB13313_A8Hfc.seq check
          >04392_13313_A8Hf_CMV_Forward_C01_03_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNGGGCGGTA NGCGTGTACG GTGGGNGGTC TATATAAGCA
             61 GAGCTCTCTG GCTAACTAGA GAACCCACTG CTTACTGGCT TATCGAAATT AATACGACTC
            121 ACTATAGGGA GACCCAAGCT GGCTAGCGTT TAAACTTAAG CTTACATGTG GTACCGAGCT
            181 CGGATCCTGA GAACTTCAGG GTGAGTCTAT GGGACCCTTG ATGTTTTCTT TCCCCTTCTT
            241 TTCTATGGTT AAGTTCATGT CATAGGAAGG GGAGAAGTAA CAGGGTACAC ATATTGACCA
            301 AATCAGGGTA ATTTTGCATT TGTAATTTTA AAAAATGCTT TCTTCTTTTA ATATACTTTT
            361 TTGTTTATCT TATTTCTAAT ACTTTCCCTA ATCTCTTTCT TTCAGGGCAA TAATGATACA
            421 ATGTATCATG CCTCTTTGCA CCATTCTAAA GAATAACAGT GATAATTTCT GGGTTAAGGC
            481 AATAGCAATA TTTCTGCATA TAAATATTTC TGCATATAAA TTGTAACTGA TGTAAGAGGT
            541 TTCATATTGC TAATAGCAGC TACAATCNAG CTACCATTCT GCTTTTATTT TATGGTTGGG
            601 ATAANGCTGG ANTATTCTGA NTCCAAGCTA GGCCCTTTTG CTAATCATGT TCNTACCTCT
            661 TATCTTCNTC CNACAGCTCC TGGNCNACGT NCTGGNNNGN NNGCTNGNCC NNTCANNTNN
          721 NNNANNNNNT NNNNNNNNNN
          //
          Primer: T7_20bp
          Region: human beta-globin IVS,insert 5'
          Sequence file: RDB13313_A8Hfd.seq check
          >04392_13313_A8Hf_T7_20bp_A05_02_ABI24.ab1
              1 CGTGCCAGCG TTTTACTTAA GCTTACATGT GGTACCGAGC TCGGATCCTG AGAACTTCAG
             61 GGTGAGTCTA TGGGACCCTT GATGTTTTCT TTCCCCTTCT TTTCTATGGT TAAGTTCATG
            121 TCATAGGAAG GGGAGAAGTA ACAGGGTACA CATATTGACC AAATCAGGGT AATTTTGCAT
            181 TTGTAATTTT AAAAAATGCT TTCTTCTTTT AATATACTTT TTTGTTTATC TTATTTCTAA
            241 TACTTTCCCT AATCTCTTTC TTTCAGGGCA ATAATGATAC AATGTATCAT GCCTCTTTGC
            301 ACCATTCTAA AGAATAACAG TGATAATTTC TGGGTTAAGG CAATAGCAAT ATTTCTGCAT
            361 ATAAATATTT CTGCATATAA ATTGTAACTG ATGTAAGAGG TTTCATATTG CTAATAGCAG
            421 CTACAATCCA GCTACCATTC TGCTTTTATT TTATGGTTGG GATAAGGCTG GATTATTCTG
            481 AGTCCAAGCT AGGCCCTTTT GCTAATCATG TTCATACCTC TTATCTTCCT CCCACAGCTC
            541 CTGGGCAACG TGCTGGTCTG TGTGCTGGCC CATCACTTTG GCAAAGAATT CTGACACTAT
            601 GAAGTGCCTT TTGTACTTAG CCTTTTTATT CATTGGGGTG AATTGCAAGT TCACCATAGT
            661 TTTTCCACAC AACCAAAAAG GAAACTGGAA AAATGTTCCT TCTAATTACC ATTATTGCCC
            721 GTCAAGCTCA GATTTAAATT GGCATAATGA CTTAATAGGC ACAGCCTTAC AAGTCAAAAT
            781 GCCCAAGAGT CACAAGGCTA TTCAAGCAGA CGGTTGGATG TGTCATGCTT CCAAATGGGT
            841 CACTACTTGT GATTTCCGCT GGTATGGACC GAAGTATATA ACACATTCCA TCCGATCCTT
            901 CACTCCATCT GTAGAACAAT GCAAGGAAAG CATTGAACAA ACGAAACAAG GAAACTTGGC
            961 TGAATCCAGG CTTCCCTCCT CAAAGTTGTG GATATGCAAC TGTGACGGAT GCCGAAGCAG
           1021 TGATTGTCCA GGTGACTCCC TCACCAATGT GCTGGTTGAA TGAATACACA AGGAGAATGG
           1081 GTTGATTCAC AGTCATCAAC GGGAAATGCA GCATAACATA TGCCCACTGT TCAATAAACT
          1141 TCTTACCACC CTGGGGCAAT T
          //
          Primer: BGH_rev
          Region: insert 3'
          Sequence file: RDB13313_A8Hfe.seq check
          >04392_13313_A8Hf_BGH_rev_B05_05_ABI24.ab1
              1 CTTACGGGAC TCTAGACTCG AGCGGCCGCC ACTGTGCTGG ATATCTGCAG AATTCCGTTT
             61 TTTTTTTTTT TTTTCATAAA AATTAAAAAC TCAAATATAA TTGAGGCCTC TTTGAGCATG
            121 GTATCACAAG TTGATTTGGT CCAAACATGA AGAATCTGTT GTGCAGGATT TGAGTTACTT
            181 TCCAAGTCGG TTCATCTCTA TGTCTGTATA AATCTGTCTT TTCTTGGTGT GCTTTAATTT
            241 AATGCAAAGA TGGATACCAA CTCGGAGAAC CAAGAATAGT CCAATGATTA ACCCTATGAT
            301 AAAGAAAAAA GAGGCAATAG AGCTTTTCCA ACTACTGAAC CAACCTTCTA CAAGCTCGAT
            361 TGGATTTTTG GATAGCCCAG TATCACCAAA AAATAAACTC TCATCATCAG GAAGTTGCGA
            421 AGCAGCGTCT TGAATGTGAG GATGTTCGAA CACCTGAGCC TTTGAGCTAA GATGAAGATC
            481 GGAGTCCAAC ATACCATGTC CAATCATGTA TAAAGGAAAC TTATATCCTG AACTGGTCCT
            541 CAGAACTCCA TTGGGTCCAA TTTCCACGTC TTCATATGGT GCCCAGTCAT CCCACAGTTC
            601 CCTTTCTGTG GTAGTTCCAC TGATCATTCC GACCATTCTT GAGAGGATTG GAGCAGCAAT
            661 ATCGACTCTG ATGTATCTGG TCTCAAAGTA TTTTAGGGTA CCATTGATTA TGGTGAAAGC
            721 AGGACCGGTT CCTGGGTTTT TAGGAGCAAG ATAGCTGAGA TCCACTGGAG AGATTGGAAG
            781 ACCCGCTCTG ATTTTGCTCC AGGTTTCTTG GCAGAGGGAA TAATCCAAGA TCCTCTCAAC
            841 GTCCTGAATT AGACTTACAT CCACTGAGGT CTGAGATGGA GCAGAGATAC TTGACCCTTC
            901 TGGGCATTCA GGGAATCTGG CTGCAGCAAA GAGATCCTTA TCAGCCATCT CGAACCAGAC
            961 ACCTGATGGG AGTCTGACTC CCCAATGCTT GCAGTATTGC ATTTTGCAGC CTTGCCCTCC
           1021 AGTTTCATAA GCAAAGTAGT TTACTTCTGA ACCCTGTGCC TCTTTCCCAG GGATGATAGC
          1081 TCTCCGTTCT CTGAGAGAGT GATGTCCCAT GAATGAGGTA GATTCACATA TTTATGC
          //
          Primer: bGH_rev3
          Region: bGHpA,insert 3'
          Sequence file: RDB13313_A8Hff.seq check
          >04392_13313_A8Hf_bGH_rev3_D01_04_ABI08.ab1
              1 NNNNNNNNNN NNNNGNNGNN GGGCANCAAC AGATGGCTGG CAACTAGAAG GCACAGTCGA
             61 GGCTGATCAG CGGGTTTAAA CGGGCCCTCT AGACTCGAGC GGCCGCCACT GTGCTGGATA
            121 TCTGCAGAAT TCCGTTTTTT TTTTTTTTTT TCATAAAAAT TAAAAACTCA AATATAATTG
            181 AGGCCTCTTT GAGCATGGTA TCACAAGTTG ATTTGGTCCA AACATGAANA ATCTGTTGTG
            241 CAGGATTTGA GTTACTTTCC AAGTCGGTTC ATCTCTATGT CTGTATAAAT CTGTCTTTTC
            301 TTGGTGTGCT TTAATTTAAT GCAAAGATGG ATACCAACTC GNANAACCAA NAATAGTCCA
            361 ATGATTAACC CTATGATAAA GAAAAAAGAG GCAATAGAGC TTTTCCAACT ACTGAACCAA
            421 CCTTCTACAA GCTCNATTGG ATTTTTGGAT AGCCCAGTAT CACCAAAAAA TAAACTCTCA
            481 TCATCAGGAA GTTGCGAAGC AGCGTCTTGA ATGTGAGGAT GTTCGAACAC CTGAGCCTTT
            541 GAGCTAAGAT GAAGATCGGA GTCCAACATA CCATGTCCAA TCATGTATAA AGGAAACTTA
            601 TATCCTGAAC TGGTCCTCAN AACTCNNTTG GGTCCNATTT CCACGTCTTC NTATGGNGCC
            661 NAGTCATCCC ACAGTTNCCT TTCNGNGGNA NTNNCNCNNN NNNTTNCNNA NNNTNNNNNN
          721 NNNN
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          user report Maruoka, M., Caspase cleavage releases a nuclear protein fragment that stimulates phospholipid scrambling at the plasma membrane. Mol. Cell 81 (7): 1397-1410.e9 (2021). PMID 33725486.
          user report Ryoden, Y., Functional Expression of the P2X7 ATP Receptor Requires Eros. J. Immunol. 204 (3): 559-568 (2020). PMID 31862710.
          user report Fukuda, T., Combinatorial expression of cell cycle regulators is more suitable for immortalization than oncogenic methods in dermal papilla cells. iScience 24 (1): 101929 (2020). PMID 33437932.
          user report Segawa, K., The CDC50A extracellular domain is required for forming a functional complex with and chaperoning phospholipid flippases to the plasma membrane. J. Biol. Chem. 293 (6): 2172-2182 (2018). PMID 29276178.
          user report Endo, R., TAR DNA-Binding Protein 43 and Disrupted in Schizophrenia 1 Coaggregation Disrupts Dendritic Local Translation and Mental Function in Frontotemporal Lobar Degeneration. Biol. Psychiatry. 84(7): 509-521 (2018). PMID 29752072.
          user report Ueno, N., PU.1 acts as tumor suppressor for myeloma cells through direct transcriptional repression of IRF4. Oncogene 36 (31): 4481-4497 (2017). PMID 28368411.
          user report Tomioka, I., Transgenic Monkey Model of the Polyglutamine Diseases Recapitulating Progressive Neurological Symptoms. eNeuro 4 (2). pii: ENEURO.0250-16 (2017). PMID 28374014.
          user report Ujino, S., Hepatitis C virus utilizes VLDLR as a novel entry pathway. Proc. Natl. Acad. Sci. U.S.A. 113 (1): 188-193 (2016). PMID 26699506.
          user report Shimojo, D., Rapid, efficient, and simple motor neuron differentiation from human pluripotent stem cells. Mol. Brain. 8 (1): 79 (2015). PMID 26626025.
          user report Mizutani, M., Baculovirus-Vectored Multistage Plasmodium vivax Vaccine Induces Both Protective and Transmission-Blocking Immunities against Transgenic Rodent Malaria Parasites. Infect. Immun. 82 (10): 4348-4357 (2014). PMID 25092912.
          user report Sekiya, S., Direct conversion of mouse fibroblasts to hepatocyte-like cells by defined factors. Nature 475 (7356): 390-393 (2011). PMID 21716291.
          user report Tanaka, Y., Dual Function of Histone H3 Lysine 36 Methyltransferase ASH1 in Regulation of Hox Gene Expression. PLoS One., 6 (11): e28171 (2011). PMID 22140534.
          user report Fan, J., Halogenated thymidine analogues restore the expression of silenced genes without demethylation. Cancer Res., 65 (15): 6927-6933 (2005). PMID 16061677.

          2022.05.02

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