Resource data sheet
DNA Bank Top

pCMV-VSV-G (#RDB04392)

Expression vector of VSV G protein


Drawn by SnapGene® software
Sequence (full) RDB04392hts01.seq
Remarks, protocol and/or map (pdf) RDB04392.pdf
Publication Unpublished bioresource
Test sheet RDB13313_A8Hfp1-3.pdf 
Assembled from experimentally sequenced data.
 
Clone info. Expression vector of VSV G protein, envelope protein for producing lentiviral particles. Use in conjunction with packaging vectors.
Vector backbone Plasmid
Selectable markers Amp^r
Growth conditions 37C, LB+Amp
Gene/insert name VSV G protein
Depositor|Developer Miyoshi, Hiroyuki |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose)[Word] [Example of MTA ]
Please visit Ordering instruction.[link] 
Terms and conditions for distribution The exportation of this material to foreign countries is controlled and restricted by the law of Japan. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer.
Remarks Currently unavailable outside Japan. Remember that you will be working with samples containing infectious virus.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利機関による非営利学術研究用)[Word] [MTAの記入例 ]
遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。
手続きの概要は、「レンチウイルスベクターの提供申し込み」をご覧ください。
MTAに書く使用条件 本件研究材料は、非営利機関の非営利学術研究に限って提供する。本件研究材料を利用した研究結果等を発表する際は、本件研究材料が故三好浩之博士により開発されたことを明示する。本件リソースの輸出は法令等により規制されている。
備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB04392 pCMV-VSV-G DNA solution

Ordering instruction of plasmids [in Japanese] [in English]

How to cite this biological resource

Materials & Methods section:

The pCMV-VSV-G was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB04392).

Reference section:

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

user_report Zha, L., Morphological basis of the lung adenocarcinoma subtypes. iScience 27 (5): 109742 (2024). PMID 38706836. [link to RRC of NBRP]
user_report Jargalsaikhan, B.E., The Dual-Pseudotyped Lentiviral Vector with VSV-G and Sendai Virus HN Enhances Infection Efficiency through the Synergistic Effect of the Envelope Proteins. Viruses 16 (6): 827 (2024). PMID 38932120. [link to RRC of NBRP]
user_report Miyata, Y., Membrane structure-responsive lipid scrambling by TMEM63B to control plasma membrane lipid distribution. Nat Struct Mol Biol. 2024 Oct 18. doi: 10.1038/s41594-024-01411-6. Epub ahead of print. PMID 39424995. [link to RRC of NBRP]
user_report Sakai, M., Genome-scale CRISPR-Cas9 screen identifies novel host factors as potential therapeutic targets for SARS-CoV-2 infection. bioRxiv doi: 10.1101/2023.03.06.531431 (2023).
user_report Watanabe, H., Astrocytic APOE4 genotype-mediated negative impacts on synaptic architecture in human pluripotent stem cell model. Stem Cell Reports 18 (9): 1854-1869 (2023). PMID 37657448. [link to RRC of NBRP]
user_report Guhathakurta, S., COX17 acetylation via MOF-KANSL complex promotes mitochondrial integrity and function. Nat. Metab. 5 (11): 1931-1952 (2023). PMID 37813994. [link to RRC of NBRP]
user_report Ryoden, Y., Requirement of Xk and Vps13a for the P2X7-mediated phospholipid scrambling and cell lysis in mouse T cells. Proc. Natl. Acad. Sci. U.S.A. 119 (7): e2119286119 (2022). PMID 35140185. [link to RRC of NBRP]
user_report Nishitsuji, H., Ubiquitination of SARS-CoV-2 NSP6 and ORF7a Facilitates NF-κB Activation. mBio 13 (4): e0097122 (2022). PMID 35856559. [link to RRC of NBRP]
user_report Miyata, Y., Two types of type IV P-type ATPases independently re-establish the asymmetrical distribution of phosphatidylserine in plasma membranes. J. Biol. Chem. 298 (11): 102527 (2022). PMID 36162506. [link to RRC of NBRP]
user_report Maruoka, M., Caspase cleavage releases a nuclear protein fragment that stimulates phospholipid scrambling at the plasma membrane. Mol. Cell 81 (7): 1397-1410.e9 (2021). PMID 33725486. [link to RRC of NBRP]
user_report Borisova, E., Structurally-discovered KLF4 variants accelerate and stabilize reprogramming to pluripotency. iScience 25 (1): 103525 (2021). PMID 35106457. [link to RRC of NBRP]
user_report Ryoden, Y., Functional Expression of the P2X7 ATP Receptor Requires Eros. J. Immunol. 204 (3): 559-568 (2020). PMID 31862710. [link to RRC of NBRP]
user_report Fukuda, T., Combinatorial expression of cell cycle regulators is more suitable for immortalization than oncogenic methods in dermal papilla cells. iScience 24 (1): 101929 (2020). PMID 33437932. [link to RRC of NBRP]
user_report Segawa, K., The CDC50A extracellular domain is required for forming a functional complex with and chaperoning phospholipid flippases to the plasma membrane. J. Biol. Chem. 293 (6): 2172-2182 (2018). PMID 29276178. [link to RRC of NBRP]
user_report Endo, R., TAR DNA-Binding Protein 43 and Disrupted in Schizophrenia 1 Coaggregation Disrupts Dendritic Local Translation and Mental Function in Frontotemporal Lobar Degeneration. Biol. Psychiatry. 84(7): 509-521 (2018). PMID 29752072. [link to RRC of NBRP]
user_report Ueno, N., PU.1 acts as tumor suppressor for myeloma cells through direct transcriptional repression of IRF4. Oncogene 36 (31): 4481-4497 (2017). PMID 28368411. [link to RRC of NBRP]
user_report Tomioka, I., Transgenic Monkey Model of the Polyglutamine Diseases Recapitulating Progressive Neurological Symptoms. eNeuro 4 (2). pii: ENEURO.0250-16 (2017). PMID 28374014. [link to RRC of NBRP]
user_report Ujino, S., Hepatitis C virus utilizes VLDLR as a novel entry pathway. Proc. Natl. Acad. Sci. U.S.A. 113 (1): 188-193 (2016). PMID 26699506. [link to RRC of NBRP]
user_report Shimojo, D., Rapid, efficient, and simple motor neuron differentiation from human pluripotent stem cells. Mol. Brain. 8 (1): 79 (2015). PMID 26626025. [link to RRC of NBRP]
user_report Mizutani, M., Baculovirus-Vectored Multistage Plasmodium vivax Vaccine Induces Both Protective and Transmission-Blocking Immunities against Transgenic Rodent Malaria Parasites. Infect. Immun. 82 (10): 4348-4357 (2014). PMID 25092912. [link to RRC of NBRP]
user_report Sekiya, S., Direct conversion of mouse fibroblasts to hepatocyte-like cells by defined factors. Nature 475 (7356): 390-393 (2011). PMID 21716291. [link to RRC of NBRP]
user_report Tanaka, Y., Dual Function of Histone H3 Lysine 36 Methyltransferase ASH1 in Regulation of Hox Gene Expression. PLoS One., 6 (11): e28171 (2011). PMID 22140534. [link to RRC of NBRP]
user_report Fan, J., Halogenated thymidine analogues restore the expression of silenced genes without demethylation. Cancer Res., 65 (15): 6927-6933 (2005). PMID 16061677. [link to RRC of NBRP]