Resource data sheet
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pCMV-VSV-G

Expression vector of VSV G protein

Catalog number RDB04392
Resource name pCMV-VSV-G
Clone info. Expression vector of VSV G protein, envelope protein for producing lentiviral particles. Use in conjunction with packaging vectors.
Vector backbone Plasmid
Selectable markers Amp^r
Originator Miyoshi, Hiroyuki |

Distribution information

CLICK here for plasmid list and map. [link]
Forms for plasmid request [in Japanese] [in English]
Terms and conditions for distribution The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose.
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Remarks ((Remarks)) Remember that you will be working with samples containing infectious virus.
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Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB13313_A5Dlp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: T7_20bp RDB13313_A5Dla.seq
>04392_13313_A5Dl_T7_20bp_A03_03.ab1
    1 CGTAACATCG CTTACTTAAG CTTACATGTG GTACCGAGCT CGGATCCTGA GAACTTCAGG
   61 GTGAGTCTAT GGGACCCTTG ATGTTTTCTT TCCCCTTCTT TTCTATGGTT AAGTTCATGT
  121 CATAGGAAGG GGAGAAGTAA CAGGGTACAC ATATTGACCA AATCAGGGTA ATTTTGCATT
  181 TGTAATTTTA AAAAATGCTT TCTTCTTTTA ATATACTTTT TTGTTTATCT TATTTCTAAT
  241 ACTTTCCCTA ATCTCTTTCT TTCAGGGCAA TAATGATACA ATGTATCATG CCTCTTTGCA
  301 CCATTCTAAA GAATAACAGT GATAATTTCT GGGTTAAGGC AATAGCAATA TTTCTGCATA
  361 TAAATATTTC TGCATATAAA TTGTAACTGA TGTAAGAGGT TTCATATTGC TAATAGCAGC
  421 TACAATCCAG CTACCATTCT GCTTTTATTT TATGGTTGGG ATAAGGCTGG ATTATTCTGA
  481 GTCCAAGCTA GGCCCTTTTG CTAATCATGT TCATACCTCT TATCTTCCTC CCACAGCTCC
  541 TGGGCAACGT GCTGGTCTGT GTGCTGGCCC ATCACTTTGG CAAAGAATTC TGACACTATG
  601 AAGTGCCTTT TGTACTTAGC CTTTTTATTC ATTGGGGTGA ATTGCAAGTT CACCATAGTT
  661 TTTCCACACA ACCAAAAAGG AAACTGGAAA AATGTTCCTT CTAATTACCA TTATTGCCCG
  721 TCAAGCTCAG ATTTAAATTG GCATAATGAC TTAATAGGCA CAGCCTTACA AGTCAAAATG
  781 CCCAAGAGTC ACAAGGCTAT TCAAGCAGAC GGTTGGATGT GTCATGCTTC CAAATGGGTC
  841 ACTACTTGTG ATTTCCGCTG GTATGGACCG AAGTATATAA CACATTCCAT CCGATCCTTC
  901 ACTCCATCTG TAGAACAATG CAAGGAAAGC ATTGAACAAA CGAAACAAGG AACTTGGCTG
  961 AATCCAGGCT TCCCTCCTCA AAGTTGTGGA TATGCAACTG TGACGGGATG CCGAAGCAGT
 1021 GATTGTCCAG GTGACTCCCT CACCATGTGC TGCTTGATGA ATACACAGGA GAATGGGTTG
 1081 ATTCACAGTT TCATCAACGG GAAAATGCAG CATTACATAT GCCCACTGAT CCATAACTTC
1141 TAACAACCCT GGCCAATT
//
Primer: BGH_rev RDB13313_A5Dlb.seq
>04392_13313_A5Dl_BGH_rev_B03_06.ab1
    1 GTTCGGGGAC TCTAGACTCG AGCGGCCGCC ACTGTGCTGG ATATCTGCAG AATTCCGTTT
   61 TTTTTTTTTT TTTTCATAAA AATTAAAAAC TCAAATATAA TTGAGGCCTC TTTGAGCATG
  121 GTATCACAAG TTGATTTGGT CCAAACATGA AGAATCTGTT GTGCAGGATT TGAGTTACTT
  181 TCCAAGTCGG TTCATCTCTA TGTCTGTATA AATCTGTCTT TTCTTGGTGT GCTTTAATTT
  241 AATGCAAAGA TGGATACCAA CTCGGAGAAC CAAGAATAGT CCAATGATTA ACCCTATGAT
  301 AAAGAAAAAA GAGGCAATAG AGCTTTTCCA ACTACTGAAC CAACCTTCTA CAAGCTCGAT
  361 TGGATTTTTG GATAGCCCAG TATCACCAAA AAATAAACTC TCATCATCAG GAAGTTGCGA
  421 AGCAGCGTCT TGAATGTGAG GATGTTCGAA CACCTGAGCC TTTGAGCTAA GATGAAGATC
  481 GGAGTCCAAC ATACCATGTC CAATCATGTA TAAAGGAAAC TTATATCCTG AACTGGTCCT
  541 CAGAACTCCA TTGGGTCCAA TTTCCACGTC TTCATATGGT GCCCAGTCAT CCCACAGTTC
  601 CCTTTCTGTG GTAGTTCCAC TGATCATTCC GACCATTCTT GAGAGGATTG GAGCAGCAAT
  661 ATCGACTCTG ATGTATCTGG TCTCAAAGTA TTTTAGGGTA CCATTGATTA TGGTGAAAGC
  721 AGGACCGGTT CCTGGGTTTT TAGGAGCAAG ATAGCTGAGA TCCACTGGAG AGATTGGAAG
  781 ACCCGCTCTG ATTTTGCTCC AGGTTTCTTG GCAGAGGGAA TAATCCAAGA TCCTCTCAAC
  841 GTCCTGAATT AGACTTACAT CCACTGAGGT CTGAGATGGA GCAGAGATAC TTGACCCTTC
  901 TGGGCATTCA GGGAATCTGG CTGCAGCAAA GAGATCCTTA TCAGCCATCT CGAACCAGAC
  961 ACCTGATGGG AGTCTGACTC CCCAATGCTT GCAGTATTGC ATTTTGCAGG CCTTGCCCTC
 1021 CCAGTTTCAT AAGCAAAGTA GTTACTTCTG AACCCTTGTG CCTCCTTTCC CAGGGATGAT
1081 AGCTCTCGGT CGTCCTGAGA AGGAGTTGAT GATCCATGGG AAAATGGAAG GCCTA
//

Please visit plasmidSequencing and PCR primers for primer information.

References and tips

Electronic file

References

user report Ueno, N., PU.1 acts as tumor suppressor for myeloma cells through direct transcriptional repression of IRF4. Oncogene 36 (31): 4481-4497 (2017). PMID 28368411.
user report Tomioka, I., Transgenic Monkey Model of the Polyglutamine Diseases Recapitulating Progressive Neurological Symptoms. eNeuro 4 (2). pii: ENEURO.0250-16 (2017). PMID 28374014.
user report Ujino, S., Hepatitis C virus utilizes VLDLR as a novel entry pathway. Proc. Natl. Acad. Sci. U.S.A. 113 (1): 188-193 (2016). PMID 26699506.
user report Shimojo, D., Rapid, efficient, and simple motor neuron differentiation from human pluripotent stem cells. Mol. Brain. 8 (1): 79 (2015). PMID 26626025.
user report Mizutani, M., Baculovirus-Vectored Multistage Plasmodium vivax Vaccine Induces Both Protective and Transmission-Blocking Immunities against Transgenic Rodent Malaria Parasites. Infect. Immun. 82 (10): 4348-4357 (2014). PMID 25092912.
user report Kurotaki, D., IRF8 inhibits C/EBPalpha activity to restrain mononuclear phagocyte progenitors from differentiating into neutrophils. Nat. Commun. 5: 4978 (2014). PMID 25236377.
user report Sekiya, S., Direct conversion of mouse fibroblasts to hepatocyte-like cells by defined factors. Nature 475 (7356): 390-393 (2011). PMID 21716291.
user report Tanaka, Y., Dual Function of Histone H3 Lysine 36 Methyltransferase ASH1 in Regulation of Hox Gene Expression. PLoS One., 6 (11): e28171 (2011). PMID 22140534.
user report Fan, J., Halogenated thymidine analogues restore the expression of silenced genes without demethylation. Cancer Res., 65 (15): 6927-6933 (2005). PMID 16061677.
user report Nagata, S., METHOD OF SCREENING ATP11C OR CDC50A INHIBITOR US Patent 20,170,023,548, 2017

2017.10.23

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