Sequencing and PCR primers for primer information.
Backbone vector of shRNA expressing lentivirus construct by Gateway(R) cloning with GFP marker driven by EF-1 alpha promoter.
| Catalog number | RDB04391 |
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| Resource name | CS-RfA-EG |
| Clone info. | Backbone vector of shRNA expressing lentivirus construct by Gateway(R) cloning with GFP marker driven by EF-1 alpha promoter. Use DB3.1 (Invitrogen) or equivalent to amplify this clone. Commonly requested with pENTR4-H1 (RDB04395) for generation of shRNA expression vector and with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394) for packaging. Please note that Zeo resistance marker is not included in the lentivirus produced from this vector. |
| Vector backbone | Plasmid |
| Selectable markers | Amp^r, Cm^r, ccdB (E. coli); Zeo^r (plasmid transfection of mammalian cells). |
| Originator | Miyoshi, Hiroyuki | |
| Terms and conditions for distribution | The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe Dr. Hiroyuki Miyoshi (The Developer, the current address: Keio University) as the Developer. Dr. Miyoshi may request the RECIPIENT to report on progress and/or results obtained through the use of the BIOLOGICAL RESOURCE. |
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| Ordering | Please visit Ordering instruction. Order form [Credit Card Payment] [Bank Transfer or Check Payment] Material Transfer Agreement (MTA) [Word] |
| Remarks | Remember that you will be working with samples containing infectious virus. Use Survival2 (Invitrogen) or equivalent to amplify this clone. |
[open/close]| Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
|---|---|---|---|
| RDB04391 | CS-RfA-EG | DNA solution |
Electronic file
| Electronic file | Sequence RDB04391z.seq from Depositor |
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| Electronic file | PDF RDB04391.pdf from Depositor |
Featured content
References
| original | Miyoshi, H., Development of a self-inactivating lentivirus vector. J. Virol., 72 (10): 8150-8157 (1998). PMID 9733856. |
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User reports and related articles (go to bottom)
RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.
Data are summerized on test sheet below.
| Test sheet | RDB12134_A5Dhp1.pdf |
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Nucleotide sequence of a portion of this resource (if available).
| Primer: pAxEF_F1 | RDB12134_A5Dha.seq |
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>04391_12134_A5Dh_pAxEF_F1_A01_01.ab1 1 ATTTTTTTCT TCATTTCAGG TGTCGTGAAC ACGCTACCGG TCGCCACCAT GGTGAGCAAG 61 GGCGAGGAGC TGTTCACCGG GGTGGTGCCC ATCCTGGTCG AGCTGGACGG CGACGTAAAC 121 GGCCACAAGT TCAGCGTGTC CGGCGAGGGC GAGGGCGATG CCACCTACGG CAAGCTGACC 181 CTGAAGTTCA TCTGCACCAC CGGCAAGCTG CCCGTGCCCT GGCCCACCCT CGTGACCACC 241 CTGACCTACG GCGTGCAGTG CTTCAGCCGC TACCCCGACC ACATGAAGCA GCACGACTTC 301 TTCAAGTCCG CCATGCCCGA AGGCTACGTC CAGGAGCGCA CCATCTTCTT CAAGGACGAC 361 GGCAACTACA AGACCCGCGC CGAGGTGAAG TTCGAGGGCG ACACCCTGGT GAACCGCATC 421 GAGCTGAAGG GCATCGACTT CAAGGAGGAC GGCAACATCC TGGGGCACAA GCTGGAGTAC 481 AACTACAACA GCCACAACGT CTATATCATG GCCGACAAGC AGAAGAACGG CATCAAGGTG 541 AACTTCAAGA TCCGCCACAA CATCGAGGAC GGCAGCGTGC AGCTCGCCGA CCACTACCAG 601 CAGAACACCC CCATCGGCGA CGGCCCCGTG CTGCTGCCCG ACAACCACTA CCTGAGCACC 661 CAGTCCGCCC TGAGCAAAGA CCCCAACGAG AAGCGCGATC ACATGGTCCT GCTGGAGTTC 721 GTGACCGCCG CCGGGATCAC TCTCGGCATG GACGAGCTGT ACAAGTCCGG ACTCAGATCC 781 TACTAGTAGG ATCTCGAGGG ATCAAGCTTA TCGATAATCA ACCTCTGGAT TACAAAATTT 841 GTGAAAGATT GACTGGTATT CTTAACTATG TTGCTCCTTT TACGCTATGT GGATACGCTG 901 CTTTAATGCC TTTGTATCAT GCTATTGCTT CCCGTATGGC TTTCATTTTC TCCTCCTTGT 961 ATAAATCCTG GTTTGCTGTC TCTTTATGAG GAGTTGTGGC CCGTTGTCAG CAACGTGGCG 1021 TGTGTGCACT GTGTTTGCTG ACGCAACCCC CCCACTGGAT TGGGGGCATT GCACACTGTC 1081 AGCTCGTTTC CAGGAACTTC GCTTCCCTCC CTCCCTATTG ACTACGGCGG GAACTTCAAT 1141 TCGGCCCGG // |
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| Primer: Cm_N_rev | RDB12134_A5Dhb.seq |
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>04391_12134_A5Dh_Cm_M_R_B01_04.ab1 1 CATGAGGTTA ACTATCCCAT ATCACCAGCT CACCGTCTTT CATTGCCATA CGGAATTCCG 61 GATGAGCATT CATCAGGCGG GCAAGAATGT GAATAAAGGC CGGATAAAAC TTGTGCTTAT 121 TTTTCTTTAC GGTCTTTAAA AAGGCCGTAA TATCCAGCTG AACGGTCTGG TTATAGGTAC 181 ATTGAGCAAC TGACTGAAAT GCCTCAAAAT GTTCTTTACG ATGCCATTGG GATATATCAA 241 CGGTGGTATA TCCAGTGATT TTTTTCTCCA TTTTAGCTTC CTTAGCTCCT GAAAATCTCG 301 ACGGATCCTA ACTCAAAATC CACACATTAT ACGAGCCGGA AGCATAAAGT GTAAAGCCTG 361 GGGTGCCTAA TGCGGCCGCC ATAGTGACTG GATATGTTGT GTTTTACAGT ATTATGTAGT 421 CTGTTTTTTA TGCAAAATCT AATTTAATAT ATTGATATTT ATATCATTTT ACGTTTCTCG 481 TTCAGCTTTT TTGTACAAAC TTGTGATAAT TCAAAGTGAA TTTCTGCTGT CCCTGTAATA 541 AACCCGAAAA TTTTGAATTT TTGTAATTTG TTTTTGTAAT TCTTTAGTTT GTATGTCTGT 601 TGCTATTATG TCTACTATTC TTTCCCCTGC ACTGTACCCC CCAATCCCCC CTTTTCTTTT 661 AAAATTGTGG ATGAATACTG CCATTTGTAG ATCCGTTCAC TAATCGAATG GATCTGTCTC 721 TGTCTCTCTC TCCACCTTCT TCTTCTATTC CTTCGGGCCT GTCGGGTCCC CTCGGGGTTG 781 GGAGGTGGGT CTGAAACGAT AATGGTGAAT ATCCCTGCCT AACTCTATTC ACTATAGAAA 841 GTACAGCAAA AACTATTCTT AAACCTACCA AGCCTCCTAC TATCATTATG AATAATTTTA 901 TATACCACAG CCAATTTGTT ATGTTAAACC AATTCCACAA ACTTGCCCAT TTATCTAATT 961 CCAATAATTC TTGTTCATTC TTTTCTTGCT GGTTTTGCGA TTCTTCAATT AAAGGAGTGT 1021 ATTAAGCTTT GTGTAAATTG TTTAATTTCT CTGTCCCACT CCATCCCAGG GTCGTGTTGA 1081 TTTCTAATCT GTTCCAGAGA TTTTATAACT CGACCTAGCC AATCTAGCAC AGCAGTTGGT 1141 GTGCCCAAAA TGGAGTTTTT TCCGGAGAGA GAC // |
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| Primer: EF1a-N_R | RDB12134_A5Dhc.seq |
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>04391_12134_A5Dh_EF1a-N_R_C01_07.ab1 1 CCTCTGGGGG ATGTGGGCGA TGTGCGCTCT GCCCACTGAC GGGCACCGGA GCCTCACGAA 61 GCTTGAATTA TCACCACTTT GTACAAGAAA GCTGAACGAG AAACGTAAAA TGATATAAAT 121 ATCAATATAT TAAATTAGAT TTTGCATAAA AAACAGACTA CATAATACTG TAAAACACAA 181 CATATCCAGT CACTATGGTC GACCTGCAGA CTGGCTGTGT ATAAGGGAGC CTGACATTTA 241 TATTCCCCAG AACATCAGGT TAATGGCGTT TTTGATGTCA TTTTCGCGGT GGCTGAGATC 301 AGCCACTTCT TCCCCGATAA CGGAGACCGG CACACTGGCC ATATCGGTGG TCATCATGCG 361 CCAGCTTTCA TCCCCGATAT GCACCACCGG GTAAAGTTCA CGGGAGACTT TATCTGACAG 421 CAGACGTGCA CTGGCCAGGG GGATCACCAT CCGTCGCCCG GGCGTGTCAA TAATATCACT 481 CTGTACATCC ACAAACAGAC GATAACGGCT CTCTCTTTTA TAGGTGTAAA CCTTAAACTG 541 CATTTCACCA GCCCCTGTTC TCGTCAGCAA AAGAGCCGTT CATTTCAATA AACCGGGCGA 601 CCTCAGCCAT CCCTTCCTGA TTTTCCGCTT TCCAGCGTTC GGCACGCAGA CGACGGGCTT 661 CATTCTGCAT GGTTGTGCTT ACCAGACCGG AGATATTGAC ATCATATATG CCTTGAGCAA 721 CTGATAGCTG TCGCTGTCAA CTGTCACTGT AATACGCTGC TTCATAGCAT ACCTCTTTTT 781 GACATACTTC GGGTATACAT ATCAGTATAT ATTCTTATAC CGCAAAAATC AGCGCGCAAA 841 TACGCATACT GTTATCTGGC TTTTAGTAAG CCGGATCCAC GCGTTTACGC CCCGCCCTGC 901 CACTCATCGC AGTACTGTTG TAATTCATTA AGCATTCTGC CGACATGGAA AGCCATCACA 961 AACGGCATGA TGAACCCTGA ATCGCCAGCG GCATCAGCAC CTTGTCGCCT TTGCGTATAT 1021 ATTTGCCCAT GTGAAAACGG GGGCGAGAGT GTCCATATTG CCACGTTTAA TCAAACTGGT 1081 GAACTCACCC AGGATGGCTG AACGAAACAT AATTCTCAAT AAACCTTTAG GAAAATAGGC 1141 CAGG // |
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Please visit Sequencing and PCR primers for primer information.
References
2018.10.22
GNP_filter3_RDBDEP_html_181012.pl