Please review the QC test results indicated by icon below as well as clone information before placing your order.

CS-RfA-EG

Backbone vector plasmid of shRNA expressing lentivirus construct by Gateway(R) cloning with GFP marker driven by EF-1 alpha promoter.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer.
Ordering	Please visit Ordering instruction.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA, for use for not-for-profit academic purpose)[Word] This RESOURCE contains a fluorescent protein owned or licensed by Global Life Sciences Solutions Operations UK Ltd. (Cytiva). Please refer to Schedule B and use Schedule A (download the Schedule A and Schedule B [link]).
Remarks	Remember that you will be working with samples containing infectious virus. Use Survival2 (Invitrogen) or equivalent to amplify this clone.

提供案内 (日本国内) [open/close]

提供条件	本件研究材料は、非営利機関の非営利学術研究に限って提供する。本件研究材料を利用した研究結果等を発表する際は、本件研究材料が故三好浩之博士により開発されたことを明示する。
提供依頼	手続きの詳細は、「レンチウイルスベクターの提供申し込み」をご覧ください。 提供依頼書 [Word] 提供同意書 (非営利機関による非営利学術研究用)[Word] GFPリソースを含みます。Schedule Bをご一読の上Schedule A (英語版のみ) の提出をお願いします （Schedule AとSchedule Bを入手[link])。 遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。
備考	このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。 このクローンの増殖にはSurvival2 (Invitrogen)あるいは同等の宿主を使用してください。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB18667_B1Afp1-3.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: Cm_M_R Region: RfA(CmR,attR1),cTTP_CTS Sequence file: RDB18667_B1Afg.seq
>04391_18667_B1Af_1_Cm_M_R_B01_04_ABI24.ab1     1 NNNNNNNNNN NNTCNNNNAT NNCCNGCTCN CCGTCTTTCN TTGCCNTACG GAATTCCGGA    61 TGAGCATTCA TCAGGCGGGC AAGAATGTGA ATAAAGGCCG GATAAAACTT GTGCTTATTT   121 TTCTTTACGG TCTTTAAAAA GGCCGTAATA TCCAGCTGAA CGGTCTGGTT ATAGGTACAT   181 TGAGCAACTG ACTGAAATGC CTCAAAATGT TCTTTACGAT GCCATTGGGA TATATCAACG   241 GTGGTATATC CAGTGATTTT TTTCTCCATT TTAGCTTCCT TAGCTCCTGA AAATCTCGAC   301 GGATCCTAAC TCAAAATCCA CACATTATAC GAGCCGGAAG CATAAAGTGT AAAGCCTGGG   361 GTGCCTAATG CGGCCGCCAT AGTGACTGGA TATGTTGTGT TTTACAGTAT TATGTAGTCT   421 GTTTTTTATG CAAAATCTAA TTTAATATAT TGATATTTAT ATCATTTTAC GTTTCTCGTT   481 CAGCTTTTTT GTACAAACTT GTGATAATTC AAAGTGAATT TCTGCTGTCC CTGTAATAAA   541 CCCGAAAATT TTGAATTTTT GTAATTTGTT TTTGTAATTC TTTAGTTTGT ATGTCTGNTG   601 CTATTATGTC TACTATTCNT TCCCCTGCAC TGTNNCCCCN NATCCCCCCT TTTCNTTTAA   661 AATTGNGNAN GAANANNGCC NTTTGNANAT NCNNTCACNN ANCNAANNNN NCNNTCTCTG 721 NNNNNCTNNN NNNNNNN //
Primer: PRE_rev Region: PRE,EGFP 3' Sequence file: RDB18667_B1Afh.seq
>04391_18667_B1Af_1_PRE_rev_D01_10_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNGG ANCANCATAG TTAAGAATAC CAGTCAATCT TTCACAAATT    61 TTGTAATCCA GAGGTTGATT ATCGATAAGC TTGATCCCTC GAGATCCTAC TAGTAGGATC   121 TGAGTCCGGA CTTGTACAGC TCGTCCATGC CGAGAGTGAT CCCGGCGGCG GTCACGAACT   181 CCAGCAGGAC CATGTGATCG CGCTTCTCGT TGGGGTCTTT GCTCAGGGCG GACTGGGTGC   241 TCAGGTAGTG GTTGTCGGGC AGCAGCACGG GGCCGTCGCC GATGGGGGTG TTCTGCTGGT   301 AGTGGTCGGC GAGCTGCACG CTGCCGTCCT CGATGTTGTG GCGGATCTTG AAGTTCACCT   361 TGATGCCGTT CTTCTGCTTG TCGGCCATGA TATAGACGTT GTGGCTGTTG TAGTTGTACT   421 CCAGCTTGTG CCCCAGGATG TTGCCGTCCT CCTTGAAGTC GATGCCCTTC AGCTCGATGC   481 GGTTCACCAG GGTGTCGCCC TCGAACTTCA CCTCGGCGCG GGTCTTGTAG TTGCCGTCGT   541 CCTTGAAGAA GANGGTGCGC TCCTGGACGT AGCCTTCGGG CATGGCGGAC TTGAAGAAGT   601 CGTGCTGCTT CNTGTGGTCN GGGTAGCGGC TGAAGCACTG CNCGCCNTNN NCNNNGNTGG   661 TCNCNANGGT GGGCCANGGN ACGGGCAGNT TGNNNGNGNN GNNNANGANN NTNNNGNNNN 721 NNTTGNCNNN NNNNGNNNNN NNNCNNNNNC NNN //
Primer: EF1a-N_R Region: EF-1 alpha pro,RfA(attR2,ccdB) Sequence file: RDB18667_B1Afi.seq
>04391_18667_B1Af_1_EF1a-N_R_A01_01_ABI24.ab1     1 NNNNNNNNNN NNNNNNGGCG ANGTGCGCTC TGCCNNCTGA CGGGCACCGG ANCCTCACGA    61 AGCTTGAATT ATCACCACTT TGTACAAGAA AGCTGAACGA GAAACGTAAA ATGATATAAA   121 TATCAATATA TTAAATTAGA TTTTGCATAA AAAACAGACT ACATAATACT GTAAAACACA   181 ACATATCCAG TCACTATGGT CGACCTGCAG ACTGGCTGTG TATAAGGGAG CCTGACATTT   241 ATATTCCCCA GAACATCAGG TTAATGGCGT TTTTGATGTC ATTTTCGCGG TGGCTGAGAT   301 CAGCCACTTC TTCCCCGATA ACGGAGACCG GCACACTGGC CATATCGGTG GTCATCATGC   361 GCCAGCTTTC ATCCCCGATA TGCACCACCG GGTAAAGTTC ACGGGAGACT TTATCTGACA   421 GCAGACGTGC ACTGGCCAGG GGGATCACCA TCCGTCGCCC GGGCGTGTCA ATAATATCAC   481 TCTGTACATC CACAAACAGA CGATAACGGC TCTCTCTTTT ATAGGTGTAA ACCTTAAACT   541 GCATTTCACC AGCCCCTGTT CTCGTCAGCA AAAGAGCCGT TCATTTCAAT AAACCGGGCG   601 ACCTCAGCCA TCCCTTCCTG ANTTTCCGCT TTCCAGCGTT CGGCACGCAG ANGANGGGCT   661 TCATTCTGCN TGGTTGNGCT TANNNNANCN GNANANANTG ACNTCATATA TGCNNNGAGC 721 AACTGANANC NGTCNNNNNN //
Primer: EF1a-C_F Region: EF-1 alpha pro,EGFP 5' Sequence file: RDB18667_B1Afj.seq
>04391_18667_B1Af_1_EF1a-C_F_C01_07_ABI24.ab1     1 NNNNNNNNNN NNNTTTNANT TTGGNNCTTG GTTNNTTCTC NAGCCTCAGA CAGTGGTTCA    61 AAGTTTTTTT CTTCCATTTC AGGTGTCGTG AACACGCTAC CGGTCGCCAC CATGGTGAGC   121 AAGGGCGAGG AGCTGTTCAC CGGGGTGGTG CCCATCCTGG TCGAGCTGGA CGGCGACGTA   181 AACGGCCACA AGTTCAGCGT GTCCGGCGAG GGCGAGGGCG ATGCCACCTA CGGCAAGCTG   241 ACCCTGAAGT TCATCTGCAC CACCGGCAAG CTGCCCGTGC CCTGGCCCAC CCTCGTGACC   301 ACCCTGACCT ACGGCGTGCA GTGCTTCAGC CGCTACCCCG ACCACATGAA GCAGCACGAC   361 TTCTTCAAGT CCGCCATGCC CGAAGGCTAC GTCCAGGAGC GCACCATCTT CTTCAAGGAC   421 GACGGCAACT ACAAGACCCG CGCCGAGGTG AAGTTCGAGG GCGACACCCT GGTGAACCGC   481 ATCGAGCTGA AGGGCATCGA CTTCAAGGAG GACGGCAACA TCCTGGGGCA CAAGCTGGAG   541 TACAACTACA ACAGCCACAA CGTCTATATC ATGGCCGACA AGCAGAAGAA CGGCATCAAG   601 GTGAACTTCA AGATCCGCCA CAACATCGAG GANGGCAGCG TGCANCTCGC CNANCACTAN   661 NNGCAGAANN NCCCNNTCGG NNANNNCCCC NNGCNGCTNN CCNANNNNNN NNACCNGANN 721 NCCNNNNNNN NCNNNNNNNA NNNNCCNNNN NNNNNN //
Primer: CMV_Forward Region: 5' LTR,HIV-1 psi Sequence file: RDB18667_B1Afk.seq
>04391_18667_B1Af_1_CMV_Forward_C01_03_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNTG NNNCNNNGGG CGGTAGGCGT GTACNGNGGG AGGTCTATAT    61 AANCAGCGCG TNTTGCCTGT ACTGGGTCTC TCTGGTTAGA CCAGATCTGA GCCTGGGAGC   121 TCTCTGGCTA ACTAGGGAAC CCACTGCTTA AGCCTCAATA AAGCTTGCCT TGAGTGCTTC   181 AAGTAGTGTG TGCCCGTCTG TTGTGTGACT CTGGTAACTA GAGATCCCTC AGACCCTTTT   241 AGTCAGTGTG GAAAATCTCT AGCAGTGGCG CCCGAACAGG GACTTGAAAG CGAAAGGGAA   301 ACCAGAGGAG CTCTCTCGAC GCAGGACTCG GCTTGCTGAA GCGCGCACGG CAAGAGGCGA   361 GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT TTTGACTAGC GGAGGCTAGA AGGAGAGAGA   421 TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG GAGAATTAGA TCGCGATGGG AAAAAATTCG   481 GTTAAGGCCA GGGGGAAAGA AAAAATATAA ATTAAAACAT ATAGTATGGG CAAGCAGGGA   541 GCTAGAACGA TTCGCAGTTA ATCCTGGCCT GTTAGAAACA TCAGAAGGCT GTAGACAAAT   601 ACTGGGACAG CTACAACCAT CCCTTCAGAC AGGATCAGAA GAACTTAGAT CATTATATAA   661 TACAGTAGCA ACCCTCTATT GTGTGCATCA AAGGATAGAG ATAAAAGACA CCAAGGAAGC   721 TTTAGACAAG ATAGAGGAAG AGCAAAACAA AAGTAAGANC ACCGCACAGC AAGCGGCCGC   781 TGATCTTCAG ACCTGGAGGA NGAGATATGA NGGACAATTG GAGAAGTGAA TTATATAAAT   841 ATAAAGTAGT AAAAATTGAA CCATTANGAG TAGCACCCAC CAAGGCAAAG ANAAGAGTGG   901 TGCAGANAGA AAAAAGAGCA GNGGGAATAN GANCTTTGTT CCTNNGNNNC NNGGGNNCNN 961 CNNNNANNCA CTATGGNNNC ANCGNNNNNG NNNCNGNNNN NNCNNNNCNN NNNNNNNN //
Primer: HIVgp_R1 Region: HIV-1 psi Sequence file: RDB18667_B1Afl.seq
>04391_18667_B1Af_1_HIVgp_R1_E01_13_ABI24.ab1     1 NNNNNNNNNN NNNNNNCTCT TTTTTCTCTC TGCACCNCTC TTCTCTTTGC CTTGGTGGGT    61 GCTACTCCTA ATGGTTCAAT TTTTACTACT TTATATTTAT ATAATTCACT TCTCCAATTG   121 TCCCTCATAT CTCCTCCTCC AGGTCTGAAG ATCAGCGGCC GCTTGCTGTG CGGTGGTCTT   181 ACTTTTGTTT TGCTCTTCCT CTATCTTGTC TAAAGCTTCC TTGGTGTCTT TTATCTCTAT   241 CCTTTGATGC ACACAATAGA GGGTTGCTAC TGTATTATAT AATGATCTAA GTTCTTCTGA   301 TCCTGTCTGA AGGGATGGTT GTAGCTGTCC CAGTATTTGT CTACAGCCTT CTGATGTTTC   361 TAACAGGCCA GGATTAACTG CGAATCGTTC TAGCTCCCTG CTTGCCCATA CTATATGTTT   421 TAATTTATAT TTTTTCTTTC CCCCTGGCCT TAACCGAATT TTTTCCCATC GCGATCTAAT   481 TCTCCCCCGC TTAATACTGA CGCTCTCGCA CCCATCTCTC TCCTTCTAGC CTCCGCTAGT   541 CAAAATTTTT GGCGTACTCA CCAGTCGCCG CCCCTCGCCT CTTGCCGTGC GCGCTTCAGC   601 AAGCCGAGTC CTGCGTCGAG AGAGCTCCTC TGGTTTCCCT TTCNCTTTCN AGTCCCTGTT   661 CNGGCGNNNC NGCTANAGAN TTTNCNCACT GANTAAANNN NCTGNNGNNN CTCTANNNAN 721 NNNANTCNCN CNNNNNANNG NNNNNNNNNN //
Primer: bGH_rev3 Region: bGH pA,3' LTR Sequence file: RDB18667_B1Afm.seq
>04391_18667_B1Af_1_bGH_rev3_F01_16_ABI24.ab1     1 NNNNNNNNNN NNNGGNNGNN GGGCNNACNA CAGATGGCTG GCAACTAGAA GGCACAGTCG    61 AGGCTGATCA GCGGGTTTAA ACGGGCCCTG CTAGAGATTT TCCACACTGA CTAAAAGGGT   121 CTGAGGGATC TCTAGTTACC AGAGTCACAC AACAGACGGG CACACACTAC TTGAAGCACT   181 CAAGGCAAGC TTTATTGAGG CTTAAGCAGT GGGTTCCCTA GTTAGCCAGA GAGCTCCCAG   241 GCTCAGATCT GGTCTAACCA GAGAGACCCA GTACAGTCCG GATGCAGCTC TCGGGCCATG   301 TGATGAAATG CTAGGCGGCT GTCAAACCTC CACTCTAATA CTTCTCTCTC CGGGTCATCC   361 ATCCCATGCA GGCTCACAGG GTGTAACAAG CGGGTGTTCT CTCCTTCATT GGCTTCTTCT   421 ACCTTCTCTT GCTCAACTGG TACTAGCTTG TAGCACCATC CAAAGGTCAG TGGATATCTG   481 ATCCCTGGCC CTGGTGTGTA GTTCTGCCAA TCAGGGAAGT AGCCTTGTGT GTGGTAGATC   541 CACAGATCAA GGATATCTTG TCTTCGTTGG GAGTGAATTA GCCCTTCCNG TCCCCCCTTT   601 TCTTTTAAAA AGTGGCTAAN ATCTACAGCT GCCTTGTAAG TCNTTGGTCT TAAAGGNANC   661 TGANGTGTGA NTGNAAAANC NNNCTNCTCC TNCTCTNGNG CNTNTANNNN NGCNCNNNCN 721 NNANTNGNNA NCTGCNNNNN NNNNN //
Primer: SV40pro_ori_F Region: SV40 pro_ori,Zeo Sequence file: RDB18667_B1Afn.seq
>04391_18667_B1Af_1_SV40pro_ori_F_G01_19_ABI24.ab1     1 NNNNNNGNNN NNGCCGCCTC TGCCTCTGAN CTATTCCNGA AGTAGTGAGG AGGCTTTTTT    61 GGAGGCCTAG GCTTTTGCAA AAAGCTCCCG GGAGCTTGTA TATCCATTTT CGGATCTGAT   121 CAGCACGTGT TGACAATTAA TCATCGGCAT AGTATATCGG CATAGTATAA TACGACAAGG   181 TGAGGAACTA AACCATGGCC AAGTTGACCA GTGCCGTTCC GGTGCTCACC GCGCGCGACG   241 TCGCCGGAGC GGTCGAGTTC TGGACCGACC GGCTCGGGTT CTCCCGGGAC TTCGTGGAGG   301 ACGACTTCGC CGGTGTGGTC CGGGACGACG TGACCCTGTT CATCAGCGCG GTCCAGGACC   361 AGGTGGTGCC GGACAACACC CTGGCCTGGG TGTGGGTGCG CGGCCTGGAC GAGCTGTACG   421 CCGAGTGGTC GGAGGTCGTG TCCACGAACT TCCGGGACGC CTCCGGGCCG GCCATGACCG   481 AGATCGGCGA GCAGCCGTGG GGGCGGGAGT TCGCCCTGCG CGACCCGGCC GGCAACTGCG   541 TGCACTTCGT GGCCGAGGAG CAGGACTGAC ACGTGCTACG AGATTTCGAT TCCACCGCCG   601 CCTTCTATGA AAGGNTGGGC TTCGGANTCG TTTTCCGGGA NGCCGGCTGG ANGATCCTCC   661 NGCGCGGGGN NCTCNTGCTG GANTTCTTCG CCCACCCNNN NNNGNTTATN GNNNCTTNNN 721 NNGGNNNNNA NTAANNCNNN NNNNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2022.06.20

GNP_filter3_RDBDEP_html_220520.pl