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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

CS-RfA-EG

Backbone vector plasmid of shRNA expressing lentivirus construct by Gateway(R) cloning with GFP marker driven by EF-1 alpha promoter.

Catalog number RDB04391
Resource name CS-RfA-EG
Clone info. Backbone vector plasmid of shRNA expressing lentivirus construct by Gateway(R) cloning with GFP marker driven by EF-1 alpha promoter.
Commonly requested with pENTR4-H1 (RDB04395) for generation of shRNA expression vector and with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394) for packaging. Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Vector backbone Plasmid
Selectable markers Please note that Zeo resistance marker is not included in the lentivirus produced from this vector. E. coli: Amp^r, Cm^r, ccdB.
Growth remarks Use DB3.1, ccdB Survival or equivalent to amplify this clone. 30 degC is fine.
Depositor|Developer Miyoshi, Hiroyuki |
Lentiviral plasmid list, maps and sequences information. [link]

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer.
Ordering Please visit Ordering instruction.[link] 
Order form 
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Material Transfer Agreement (MTA, for use for not-for-profit academic purpose)[Word]
Schedule A (GFP-A) [Link]
Remarks Remember that you will be working with samples containing infectious virus.
Use Survival2 (Invitrogen) or equivalent to amplify this clone.
Please use Schedule A (GFP-A) additionally.
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB04391 CS-RfA-EG DNA solution

Ordering instruction of plasmids [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Electronic file Sequence RDB04391z.seq
Electronic file Remarks, protocol and/or map (pdf) RDB04391.pdf

Original reference

original Miyoshi, H., Development of a self-inactivating lentivirus vector. J. Virol., 72 (10): 8150-8157 (1998). PMID 9733856.

Further references such as user reports and related articles (go to bottom)

Featured content


Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB12134_A5Dhp1-2.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxEF_F1
Sequence file: RDB12134_A5Dha.seq check
>04391_12134_A5Dh_pAxEF_F1_A01_01.ab1
    1 ATTTTTTTCT TCATTTCAGG TGTCGTGAAC ACGCTACCGG TCGCCACCAT GGTGAGCAAG
   61 GGCGAGGAGC TGTTCACCGG GGTGGTGCCC ATCCTGGTCG AGCTGGACGG CGACGTAAAC
  121 GGCCACAAGT TCAGCGTGTC CGGCGAGGGC GAGGGCGATG CCACCTACGG CAAGCTGACC
  181 CTGAAGTTCA TCTGCACCAC CGGCAAGCTG CCCGTGCCCT GGCCCACCCT CGTGACCACC
  241 CTGACCTACG GCGTGCAGTG CTTCAGCCGC TACCCCGACC ACATGAAGCA GCACGACTTC
  301 TTCAAGTCCG CCATGCCCGA AGGCTACGTC CAGGAGCGCA CCATCTTCTT CAAGGACGAC
  361 GGCAACTACA AGACCCGCGC CGAGGTGAAG TTCGAGGGCG ACACCCTGGT GAACCGCATC
  421 GAGCTGAAGG GCATCGACTT CAAGGAGGAC GGCAACATCC TGGGGCACAA GCTGGAGTAC
  481 AACTACAACA GCCACAACGT CTATATCATG GCCGACAAGC AGAAGAACGG CATCAAGGTG
  541 AACTTCAAGA TCCGCCACAA CATCGAGGAC GGCAGCGTGC AGCTCGCCGA CCACTACCAG
  601 CAGAACACCC CCATCGGCGA CGGCCCCGTG CTGCTGCCCG ACAACCACTA CCTGAGCACC
  661 CAGTCCGCCC TGAGCAAAGA CCCCAACGAG AAGCGCGATC ACATGGTCCT GCTGGAGTTC
  721 GTGACCGCCG CCGGGATCAC TCTCGGCATG GACGAGCTGT ACAAGTCCGG ACTCAGATCC
  781 TACTAGTAGG ATCTCGAGGG ATCAAGCTTA TCGATAATCA ACCTCTGGAT TACAAAATTT
  841 GTGAAAGATT GACTGGTATT CTTAACTATG TTGCTCCTTT TACGCTATGT GGATACGCTG
  901 CTTTAATGCC TTTGTATCAT GCTATTGCTT CCCGTATGGC TTTCATTTTC TCCTCCTTGT
  961 ATAAATCCTG GTTTGCTGTC TCTTTATGAG GAGTTGTGGC CCGTTGTCAG CAACGTGGCG
 1021 TGTGTGCACT GTGTTTGCTG ACGCAACCCC CCCACTGGAT TGGGGGCATT GCACACTGTC
 1081 AGCTCGTTTC CAGGAACTTC GCTTCCCTCC CTCCCTATTG ACTACGGCGG GAACTTCAAT
1141 TCGGCCCGG
//
Primer: Cm_M_R
Sequence file: RDB12134_A5Dhb.seq check
>04391_12134_A5Dh_Cm_M_R_B01_04.ab1
    1 CATGAGGTTA ACTATCCCAT ATCACCAGCT CACCGTCTTT CATTGCCATA CGGAATTCCG
   61 GATGAGCATT CATCAGGCGG GCAAGAATGT GAATAAAGGC CGGATAAAAC TTGTGCTTAT
  121 TTTTCTTTAC GGTCTTTAAA AAGGCCGTAA TATCCAGCTG AACGGTCTGG TTATAGGTAC
  181 ATTGAGCAAC TGACTGAAAT GCCTCAAAAT GTTCTTTACG ATGCCATTGG GATATATCAA
  241 CGGTGGTATA TCCAGTGATT TTTTTCTCCA TTTTAGCTTC CTTAGCTCCT GAAAATCTCG
  301 ACGGATCCTA ACTCAAAATC CACACATTAT ACGAGCCGGA AGCATAAAGT GTAAAGCCTG
  361 GGGTGCCTAA TGCGGCCGCC ATAGTGACTG GATATGTTGT GTTTTACAGT ATTATGTAGT
  421 CTGTTTTTTA TGCAAAATCT AATTTAATAT ATTGATATTT ATATCATTTT ACGTTTCTCG
  481 TTCAGCTTTT TTGTACAAAC TTGTGATAAT TCAAAGTGAA TTTCTGCTGT CCCTGTAATA
  541 AACCCGAAAA TTTTGAATTT TTGTAATTTG TTTTTGTAAT TCTTTAGTTT GTATGTCTGT
  601 TGCTATTATG TCTACTATTC TTTCCCCTGC ACTGTACCCC CCAATCCCCC CTTTTCTTTT
  661 AAAATTGTGG ATGAATACTG CCATTTGTAG ATCCGTTCAC TAATCGAATG GATCTGTCTC
  721 TGTCTCTCTC TCCACCTTCT TCTTCTATTC CTTCGGGCCT GTCGGGTCCC CTCGGGGTTG
  781 GGAGGTGGGT CTGAAACGAT AATGGTGAAT ATCCCTGCCT AACTCTATTC ACTATAGAAA
  841 GTACAGCAAA AACTATTCTT AAACCTACCA AGCCTCCTAC TATCATTATG AATAATTTTA
  901 TATACCACAG CCAATTTGTT ATGTTAAACC AATTCCACAA ACTTGCCCAT TTATCTAATT
  961 CCAATAATTC TTGTTCATTC TTTTCTTGCT GGTTTTGCGA TTCTTCAATT AAAGGAGTGT
 1021 ATTAAGCTTT GTGTAAATTG TTTAATTTCT CTGTCCCACT CCATCCCAGG GTCGTGTTGA
 1081 TTTCTAATCT GTTCCAGAGA TTTTATAACT CGACCTAGCC AATCTAGCAC AGCAGTTGGT
1141 GTGCCCAAAA TGGAGTTTTT TCCGGAGAGA GAC
//
Primer: EF1a-N_R
Sequence file: RDB12134_A5Dhc.seq check
>04391_12134_A5Dh_EF1a-N_R_C01_07.ab1
    1 CCTCTGGGGG ATGTGGGCGA TGTGCGCTCT GCCCACTGAC GGGCACCGGA GCCTCACGAA
   61 GCTTGAATTA TCACCACTTT GTACAAGAAA GCTGAACGAG AAACGTAAAA TGATATAAAT
  121 ATCAATATAT TAAATTAGAT TTTGCATAAA AAACAGACTA CATAATACTG TAAAACACAA
  181 CATATCCAGT CACTATGGTC GACCTGCAGA CTGGCTGTGT ATAAGGGAGC CTGACATTTA
  241 TATTCCCCAG AACATCAGGT TAATGGCGTT TTTGATGTCA TTTTCGCGGT GGCTGAGATC
  301 AGCCACTTCT TCCCCGATAA CGGAGACCGG CACACTGGCC ATATCGGTGG TCATCATGCG
  361 CCAGCTTTCA TCCCCGATAT GCACCACCGG GTAAAGTTCA CGGGAGACTT TATCTGACAG
  421 CAGACGTGCA CTGGCCAGGG GGATCACCAT CCGTCGCCCG GGCGTGTCAA TAATATCACT
  481 CTGTACATCC ACAAACAGAC GATAACGGCT CTCTCTTTTA TAGGTGTAAA CCTTAAACTG
  541 CATTTCACCA GCCCCTGTTC TCGTCAGCAA AAGAGCCGTT CATTTCAATA AACCGGGCGA
  601 CCTCAGCCAT CCCTTCCTGA TTTTCCGCTT TCCAGCGTTC GGCACGCAGA CGACGGGCTT
  661 CATTCTGCAT GGTTGTGCTT ACCAGACCGG AGATATTGAC ATCATATATG CCTTGAGCAA
  721 CTGATAGCTG TCGCTGTCAA CTGTCACTGT AATACGCTGC TTCATAGCAT ACCTCTTTTT
  781 GACATACTTC GGGTATACAT ATCAGTATAT ATTCTTATAC CGCAAAAATC AGCGCGCAAA
  841 TACGCATACT GTTATCTGGC TTTTAGTAAG CCGGATCCAC GCGTTTACGC CCCGCCCTGC
  901 CACTCATCGC AGTACTGTTG TAATTCATTA AGCATTCTGC CGACATGGAA AGCCATCACA
  961 AACGGCATGA TGAACCCTGA ATCGCCAGCG GCATCAGCAC CTTGTCGCCT TTGCGTATAT
 1021 ATTTGCCCAT GTGAAAACGG GGGCGAGAGT GTCCATATTG CCACGTTTAA TCAAACTGGT
 1081 GAACTCACCC AGGATGGCTG AACGAAACAT AATTCTCAAT AAACCTTTAG GAAAATAGGC
1141 CAGG
//
Primer: HIVgp_R1
Sequence file: RDB12134_A5Dhd.seq check
>04391_12134_A9FlF_HIVgp_R1_F02_06_ABI08.ab1
    1 CAAACCCTTC TATTTTCCAC TGCTCTTTTT TCTCTCTGCA CCACTCTTCT CTTTGCCTTG
   61 GTGGGTGCTA CTCCTAATGG TTCAATTTTT ACTACTTTAT ATTTATATAA TTCACTTCTC
  121 CAATTGTCCC TCATATCTCC TCCTCCAGGT CTGAAGATCA GCGGCCGCTT GCTGTGCGGT
  181 GGTCTTACTT TTGTTTTGCT CTTCCTCTAT CTTGTCTAAA GCTTCCTTGG TGTCTTTTAT
  241 CTCTATCCTT TGATGCACAC AATAGAGGGT TGCTACTGTA TTATATAATG ATCTAAGTTC
  301 TTCTGATCCT GTCTGAAGGG ATGGTTGTAG CTGTCCCAGT ATTTGTCTAC AGCCTTCTGA
  361 TGTTTCTAAC AGGCCAGGAT TAACTGCGAA TCGTTCTAGC TCCCTGCTTG CCCATACTAT
  421 ATGTTTTAAT TTATATTTTT TCTTTCCCCC TGGCCTTAAC CGAATTTTTT CCCATCGCGA
  481 TCTAATTCTC CCCCGCTTAA TACTGACGCT CTCGCACCCA TCTCTCTCCT TCTAGCCTCC
  541 GCTAGTCAAA ATTTTTGGCG TACTCACCAG TCGCCGCCCC TCGCCTCTTG CCGTGCGCGC
  601 TTCAGCAAGC CGAGTCCTGC GTCGAGAGAG CTCCTCTGGT TTCCCTTTCG CTTTCAAGTC
  661 CCTGTTCGGG CGCCACTGCT AGAGATTTTC CACACTGACT AAAAGGGTCT GAGGGATCTC
  721 TAGTTACCAG AGTCACACAA CAGACGGGCA CACACTACTT GAAGCACTCA AGGCAAGCTT
  781 TATTGAGGCT TAAGCAGTGG GTTCCCTAGT TAGCCAGAGA GCTCCCAGGC TCAGATCTGG
  841 TCTAACCAGA GAGACCCAGT ACAGGCAAAA CGCGCTGCTT ATATAGACCT CCCACCGTAC
  901 ACGCCTACCG CCCATTTGCG TCAATGGGGG CGGAGTTGTT ACGACATTTT GGAAAAGTCC
  961 CGTTGATTTT GGTGCCCAAA ACAAACTCCC ATTTGACGTC AATGGGGGTG GGAGACTTGG
 1021 AATCCCCGTG AGTCAAACCG GCTATCCACG CCCATTGATG TACTGCCAAA ACCGGCATCA
 1081 ACCATGGTAA TAGCGGATGA ACTAATTACG TAGATGTACT GGCCAAGTAG GAAAGGTTCC
1141 ATAGGGTCAT GTACTGGGGG CATATTGTCA GGGGCGGGGT CT
//
Primer: BGH_rev2
Sequence file: RDB12134_A5Dhe.seq check
>04391_12134_A9FlF_BGH_rev2_E02_05_ABI08.ab1
    1 GATTCAGGGT TAGGGAAGCA CGGGGGAGGG GCAACAACAG ATGGCTGGCA ACTAGAAGGC
   61 ACAGTCGAGG CTGATCAGCG GGTTTAAACG GGCCCTGCTA GAGATTTTCC ACACTGACTA
  121 AAAGGGTCTG AGGGATCTCT AGTTACCAGA GTCACACAAC AGACGGGCAC ACACTACTTG
  181 AAGCACTCAA GGCAAGCTTT ATTGAGGCTT AAGCAGTGGG TTCCCTAGTT AGCCAGAGAG
  241 CTCCCAGGCT CAGATCTGGT CTAACCAGAG AGACCCAGTA CAGTCCGGAT GCAGCTCTCG
  301 GGCCATGTGA TGAAATGCTA GGCGGCTGTC AAACCTCCAC TCTAATACTT CTCTCTCCGG
  361 GTCATCCATC CCATGCAGGC TCACAGGGTG TAACAAGCGG GTGTTCTCTC CTTCATTGGC
  421 TTCTTCTACC TTCTCTTGCT CAACTGGTAC TAGCTTGTAG CACCATCCAA AGGTCAGTGG
  481 ATATCTGATC CCTGGCCCTG GTGTGTAGTT CTGCCAATCA GGGAAGTAGC CTTGTGTGTG
  541 GTAGATCCAC AGATCAAGGA TATCTTGTCT TCGTTGGGAG TGAATTAGCC CTTCCAGTCC
  601 CCCCTTTTCT TTTAAAAAGT GGCTAAGATC TACAGCTGCC TTGTAAGTCA TTGGTCTTAA
  661 AGGTACCTGA GGTGTGACTG GAAAACCCAC CTCCTCCTCC TCTTGTGCTT CTAGCCAGGC
  721 ACAATCAGCA TTGGTAGCTG CTGTATTGCT ACTTGTGATT GCTCCATGTT TTTCTAGGTC
  781 TCGACGGTAT CGATGCGGGG AGGCGGCCCA AAGGGAGATC CGACTCGTCT GAGGGCGAAG
  841 GCGAAGACGC GGAAGAGGCC GCAGAGCCGG CAGCAGCCGC GGGAAGGAAG GTCCGCTGGA
  901 TTGAGGGCCG AAGGGACGTA GCAGAAGGAC GTCCCGCGCA GAATCCAGGT GGCAACACAG
  961 GCGAGCAGCC CATGGAAAGG ACGTCAGCTT CCCCGACACA CCACGGGAAT TTGTCAGTGC
 1021 CCAACAGCCG AGCCCTGTCC AGCAGCCGGG CAGCAGGCGC GATGAGTTCG CGTTGCAATA
 1081 GGAGGGGAAG CGAAGTTCCG AAAGAGCCTG AACGGTGATG CCAATGCCTC AATCATGGAT
1141 GCGTCAGTCA GATGTCG
//
Primer: SV40pro_F_V2
Sequence file: RDB12134_A5Dhf.seq check
>04391_12134_A9FlF_SV40pro_F_V2_G02_07_ABI08.ab1
    1 GGTGAACCTT TTTTTGGAGG CCTAGGGCTT TTGCAAAAGC TCCCGGGGAG CTTGTATATC
   61 CATTTTCGGA TCTGATCAGC ACGTGTTGAC AATTAATCAT CGGCATAGTA TATCGGCATA
  121 GTATAATACG ACAAGGTGAG GAACTAAACC ATGGCCAAGT TGACCAGTGC CGTTCCGGTG
  181 CTCACCGCGC GCGACGTCGC CGGAGCGGTC GAGTTCTGGA CCGACCGGCT CGGGTTCTCC
  241 CGGGACTTCG TGGAGGACGA CTTCGCCGGT GTGGTCCGGG ACGACGTGAC CCTGTTCATC
  301 AGCGCGGTCC AGGACCAGGT GGTGCCGGAC AACACCCTGG CCTGGGTGTG GGTGCGCGGC
  361 CTGGACGAGC TGTACGCCGA GTGGTCGGAG GTCGTGTCCA CGAACTTCCG GGACGCCTCC
  421 GGGCCGGCCA TGACCGAGAT CGGCGAGCAG CCGTGGGGGC GGGAGTTCGC CCTGCGCGAC
  481 CCGGCCGGCA ACTGCGTGCA CTTCGTGGCC GAGGAGCAGG ACTGACACGT GCTACGAGAT
  541 TTCGATTCCA CCGCCGCCTT CTATGAAAGG TTGGGCTTCG GAATCGTTTT CCGGGACGCC
  601 GGCTGGATGA TCCTCCAGCG CGGGGATCTC ATGCTGGAGT TCTTCGCCCA CCCCAACTTG
  661 TTTATTGCAG CTTATAATGG TTACAAATAA AGCAATAGCA TCACAAATTT CACAAATAAA
  721 GCATTTTTTT CACTGCATTC TAGTTGTGGT TTGTCCAAAC TCATCAATGT ATCTTATCAT
  781 GTCTGTATAC CGTCGACCTC TAGCTAGAGC TTGGCGTAAT CATGGTCATA GCTGTTTCCT
  841 GTGTGAAATT GTTATCCGCT CACAATTCCA CACAACATAA CGAGCCGGAA GCATAAAGTG
  901 TAAAAGCCTG GGGGTGCCTA ATGAGTGAAG CTAACTCACA TTAAATTGCG TTGCGCTCAC
  961 TGCCCGCTTT CCAGTCGGGA AAACCCTGTC GTGCCAGCTG CATTTAATGA ATCGGCCCAA
 1021 CGCGCGGGGA AGAAGGCGGG TTGCCGTAAT TGGGGCGGCT CTTCCGCTTT CCTCGGCTTC
 1081 ACTTGAACTC GCTTGCGCCT CGGTTCGTTT CGGCTTGCCG ACGAGCGTAA TCCAGCTCAC
1141 CTCAAGGCGG TAAATACCGG GTTAATCCCC ACAGAAAAAT TCCAGG
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Miyoshi, H., Development of a self-inactivating lentivirus vector. J. Virol., 72 (10): 8150-8157 (1998). PMID 9733856.
user report Kawasaki, N., TUFT1 interacts with RABGAP1 and regulates mTORC1 signaling Cell Discovery 4 (1): (2018). PMID 29423269.
user report Yoshitomi, H., Human Sox4 facilitates the development of CXCL13-producing helper T cells in inflammatory environments. Nat. Commun. 9 (1): 3762 (2018). PMID 30232328.
user report Ishibashi, M., CD200-positive cancer associated fibroblasts augment the sensitivity of Epidermal Growth Factor Receptor mutation-positive lung adenocarcinomas to EGFR Tyrosine kinase inhibitors. Sci. Rep. 7: 46662 (2017). PMID 28429795.
user report Kobayashi, S., TGF-beta induces the differentiation of human CXCL13-producing CD4(+) T cells. Eur. J. Immunol. 46 (2): 360-371 (2016). PMID 26541894.
user report Katsura, A., MicroRNA-31 is a positive modulator of endothelial-mesenchymal transition and associated secretory phenotype induced by TGF-beta. Genes Cells 21 (1): 99-116 PMID 26663584.
user report Neri, S., Podoplanin-expressing cancer-associated fibroblasts lead and enhance the local invasion of cancer cells in lung adenocarcinoma. Int. J. Cancer. 137 (4): 784-796 (2015). PMID 25648219.
user report Tanaka, Y., Dual Function of Histone H3 Lysine 36 Methyltransferase ASH1 in Regulation of Hox Gene Expression. PLoS One., 6 (11): e28171 (2011). PMID 22140534.
user report Ohoka, N., The orphan nuclear receptor RORalpha restrains adipocyte differentiation through a reduction of C/EBPbeta activity and perilipin gene expression. Mol. Endocrinol., 23, 759-771 (2009). PMID 19324970.
user report Naka, H., Requirement for COUP-TFI and II in the temporal specification of neural stem cells in CNS development. Nat. Neurosci. 11 (9): 1014-1023 (2008). PMID 19160499.
user report Tsukamoto H., TCR ligand avidity determines the mode of B-Raf/Raf-1/ERK activation leading to the activation of human CD4+ T cell clone. Eur. J. Immunol., 36, 1926-1937 (2006). PMID 16791876.

2020.12.24

GNP_filter3_RDBDEP_html_201222.pl