RDB04391 - CS-RfA-EG

Resource data sheet
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CS-RfA-EG

Backbone vector plasmid of shRNA expressing lentivirus construct by Gateway(R) cloning with GFP marker driven by EF-1 alpha promoter.

Catalog number	RDB04391
Resource name	CS-RfA-EG
Clone info.	Backbone vector plasmid of shRNA expressing lentivirus construct by Gateway(R) cloning with GFP marker driven by EF-1 alpha promoter. Commonly requested with pENTR4-H1 (RDB04395) for generation of shRNA expression vector and with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394) for packaging. Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Vector backbone	Plasmid
Selectable markers	Please note that Zeo resistance marker is not included in the lentivirus produced from this vector. E. coli: Amp^r, Cm^r, ccdB.
Growth remarks	Use DB3.1, ccdB Survival or equivalent to amplify this clone. 30 degC is fine.
Depositor\|Developer	Miyoshi, Hiroyuki \|

Lentiviral plasmid list, maps and sequences information. [link]

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer.

Ordering Please visit Ordering instruction.[link]
Order form [Credit Card Payment] [Bank Transfer or Check Payment]
Material Transfer Agreement (MTA, for use for not-for-profit academic purpose)[Word]
Schedule A (GFP-A) [Link]

Remarks Remember that you will be working with samples containing infectious virus.
Use Survival2 (Invitrogen) or equivalent to amplify this clone.
Please use Schedule A (GFP-A) additionally.

Distribution information (domestic users) [open/close]

Terms and conditions set forth by the DEPOSITOR	The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer.
Ordering	Please visit Ordering instruction.[link] Order form [Credit Card Payment] [Bank Transfer or Check Payment] Material Transfer Agreement (MTA, for use for not-for-profit academic purpose)[Word] Schedule A (GFP-A) [Link]
Remarks	Remember that you will be working with samples containing infectious virus. Use Survival2 (Invitrogen) or equivalent to amplify this clone. Please use Schedule A (GFP-A) additionally.

提供条件	本件研究材料は、非営利機関の非営利学術研究に限って提供する。本件研究材料を利用した研究結果等を発表する際は、本件研究材料が故三好浩之博士により開発されたことを明示する。
提供依頼	手続きの詳細は、「レンチウイルスベクターの提供申し込み」をご覧ください。提供依頼書 [Word] 提供同意書 (非営利機関による非営利学術研究用)[Word] Schedule A（GFP-A, 英語版のみ） [Link] 遺伝子組換え生物の受入れ確認書 [Word]
備考	このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。このクローンの増殖にはSurvival2 (Invitrogen)あるいは同等の宿主を使用してください。 Schedule A（GFP-A, 英語版のみ）が追加で必要です。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB04391	CS-RfA-EG	DNA solution

Ordering instruction of plasmids [in Japanese] [in English]
Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

References and tips

Electronic file

Electronic file	Sequence RDB04391z.seq
Electronic file	Remarks, protocol and/or map (pdf) RDB04391.pdf

Original reference

original	Miyoshi, H., Development of a self-inactivating lentivirus vector. J. Virol., 72 (10): 8150-8157 (1998). PMID 9733856.

Further references such as user reports and related articles (go to bottom)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB12134_A5Dhp1-2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxEF_F1 Sequence file: RDB12134_A5Dha.seq
>04391_12134_A5Dh_pAxEF_F1_A01_01.ab1 1 ATTTTTTTCT TCATTTCAGG TGTCGTGAAC ACGCTACCGG TCGCCACCAT GGTGAGCAAG 61 GGCGAGGAGC TGTTCACCGG GGTGGTGCCC ATCCTGGTCG AGCTGGACGG CGACGTAAAC 121 GGCCACAAGT TCAGCGTGTC CGGCGAGGGC GAGGGCGATG CCACCTACGG CAAGCTGACC 181 CTGAAGTTCA TCTGCACCAC CGGCAAGCTG CCCGTGCCCT GGCCCACCCT CGTGACCACC 241 CTGACCTACG GCGTGCAGTG CTTCAGCCGC TACCCCGACC ACATGAAGCA GCACGACTTC 301 TTCAAGTCCG CCATGCCCGA AGGCTACGTC CAGGAGCGCA CCATCTTCTT CAAGGACGAC 361 GGCAACTACA AGACCCGCGC CGAGGTGAAG TTCGAGGGCG ACACCCTGGT GAACCGCATC 421 GAGCTGAAGG GCATCGACTT CAAGGAGGAC GGCAACATCC TGGGGCACAA GCTGGAGTAC 481 AACTACAACA GCCACAACGT CTATATCATG GCCGACAAGC AGAAGAACGG CATCAAGGTG 541 AACTTCAAGA TCCGCCACAA CATCGAGGAC GGCAGCGTGC AGCTCGCCGA CCACTACCAG 601 CAGAACACCC CCATCGGCGA CGGCCCCGTG CTGCTGCCCG ACAACCACTA CCTGAGCACC 661 CAGTCCGCCC TGAGCAAAGA CCCCAACGAG AAGCGCGATC ACATGGTCCT GCTGGAGTTC 721 GTGACCGCCG CCGGGATCAC TCTCGGCATG GACGAGCTGT ACAAGTCCGG ACTCAGATCC 781 TACTAGTAGG ATCTCGAGGG ATCAAGCTTA TCGATAATCA ACCTCTGGAT TACAAAATTT 841 GTGAAAGATT GACTGGTATT CTTAACTATG TTGCTCCTTT TACGCTATGT GGATACGCTG 901 CTTTAATGCC TTTGTATCAT GCTATTGCTT CCCGTATGGC TTTCATTTTC TCCTCCTTGT 961 ATAAATCCTG GTTTGCTGTC TCTTTATGAG GAGTTGTGGC CCGTTGTCAG CAACGTGGCG 1021 TGTGTGCACT GTGTTTGCTG ACGCAACCCC CCCACTGGAT TGGGGGCATT GCACACTGTC 1081 AGCTCGTTTC CAGGAACTTC GCTTCCCTCC CTCCCTATTG ACTACGGCGG GAACTTCAAT 1141 TCGGCCCGG //
Primer: Cm_M_R Sequence file: RDB12134_A5Dhb.seq
>04391_12134_A5Dh_Cm_M_R_B01_04.ab1 1 CATGAGGTTA ACTATCCCAT ATCACCAGCT CACCGTCTTT CATTGCCATA CGGAATTCCG 61 GATGAGCATT CATCAGGCGG GCAAGAATGT GAATAAAGGC CGGATAAAAC TTGTGCTTAT 121 TTTTCTTTAC GGTCTTTAAA AAGGCCGTAA TATCCAGCTG AACGGTCTGG TTATAGGTAC 181 ATTGAGCAAC TGACTGAAAT GCCTCAAAAT GTTCTTTACG ATGCCATTGG GATATATCAA 241 CGGTGGTATA TCCAGTGATT TTTTTCTCCA TTTTAGCTTC CTTAGCTCCT GAAAATCTCG 301 ACGGATCCTA ACTCAAAATC CACACATTAT ACGAGCCGGA AGCATAAAGT GTAAAGCCTG 361 GGGTGCCTAA TGCGGCCGCC ATAGTGACTG GATATGTTGT GTTTTACAGT ATTATGTAGT 421 CTGTTTTTTA TGCAAAATCT AATTTAATAT ATTGATATTT ATATCATTTT ACGTTTCTCG 481 TTCAGCTTTT TTGTACAAAC TTGTGATAAT TCAAAGTGAA TTTCTGCTGT CCCTGTAATA 541 AACCCGAAAA TTTTGAATTT TTGTAATTTG TTTTTGTAAT TCTTTAGTTT GTATGTCTGT 601 TGCTATTATG TCTACTATTC TTTCCCCTGC ACTGTACCCC CCAATCCCCC CTTTTCTTTT 661 AAAATTGTGG ATGAATACTG CCATTTGTAG ATCCGTTCAC TAATCGAATG GATCTGTCTC 721 TGTCTCTCTC TCCACCTTCT TCTTCTATTC CTTCGGGCCT GTCGGGTCCC CTCGGGGTTG 781 GGAGGTGGGT CTGAAACGAT AATGGTGAAT ATCCCTGCCT AACTCTATTC ACTATAGAAA 841 GTACAGCAAA AACTATTCTT AAACCTACCA AGCCTCCTAC TATCATTATG AATAATTTTA 901 TATACCACAG CCAATTTGTT ATGTTAAACC AATTCCACAA ACTTGCCCAT TTATCTAATT 961 CCAATAATTC TTGTTCATTC TTTTCTTGCT GGTTTTGCGA TTCTTCAATT AAAGGAGTGT 1021 ATTAAGCTTT GTGTAAATTG TTTAATTTCT CTGTCCCACT CCATCCCAGG GTCGTGTTGA 1081 TTTCTAATCT GTTCCAGAGA TTTTATAACT CGACCTAGCC AATCTAGCAC AGCAGTTGGT 1141 GTGCCCAAAA TGGAGTTTTT TCCGGAGAGA GAC //
Primer: EF1a-N_R Sequence file: RDB12134_A5Dhc.seq
>04391_12134_A5Dh_EF1a-N_R_C01_07.ab1 1 CCTCTGGGGG ATGTGGGCGA TGTGCGCTCT GCCCACTGAC GGGCACCGGA GCCTCACGAA 61 GCTTGAATTA TCACCACTTT GTACAAGAAA GCTGAACGAG AAACGTAAAA TGATATAAAT 121 ATCAATATAT TAAATTAGAT TTTGCATAAA AAACAGACTA CATAATACTG TAAAACACAA 181 CATATCCAGT CACTATGGTC GACCTGCAGA CTGGCTGTGT ATAAGGGAGC CTGACATTTA 241 TATTCCCCAG AACATCAGGT TAATGGCGTT TTTGATGTCA TTTTCGCGGT GGCTGAGATC 301 AGCCACTTCT TCCCCGATAA CGGAGACCGG CACACTGGCC ATATCGGTGG TCATCATGCG 361 CCAGCTTTCA TCCCCGATAT GCACCACCGG GTAAAGTTCA CGGGAGACTT TATCTGACAG 421 CAGACGTGCA CTGGCCAGGG GGATCACCAT CCGTCGCCCG GGCGTGTCAA TAATATCACT 481 CTGTACATCC ACAAACAGAC GATAACGGCT CTCTCTTTTA TAGGTGTAAA CCTTAAACTG 541 CATTTCACCA GCCCCTGTTC TCGTCAGCAA AAGAGCCGTT CATTTCAATA AACCGGGCGA 601 CCTCAGCCAT CCCTTCCTGA TTTTCCGCTT TCCAGCGTTC GGCACGCAGA CGACGGGCTT 661 CATTCTGCAT GGTTGTGCTT ACCAGACCGG AGATATTGAC ATCATATATG CCTTGAGCAA 721 CTGATAGCTG TCGCTGTCAA CTGTCACTGT AATACGCTGC TTCATAGCAT ACCTCTTTTT 781 GACATACTTC GGGTATACAT ATCAGTATAT ATTCTTATAC CGCAAAAATC AGCGCGCAAA 841 TACGCATACT GTTATCTGGC TTTTAGTAAG CCGGATCCAC GCGTTTACGC CCCGCCCTGC 901 CACTCATCGC AGTACTGTTG TAATTCATTA AGCATTCTGC CGACATGGAA AGCCATCACA 961 AACGGCATGA TGAACCCTGA ATCGCCAGCG GCATCAGCAC CTTGTCGCCT TTGCGTATAT 1021 ATTTGCCCAT GTGAAAACGG GGGCGAGAGT GTCCATATTG CCACGTTTAA TCAAACTGGT 1081 GAACTCACCC AGGATGGCTG AACGAAACAT AATTCTCAAT AAACCTTTAG GAAAATAGGC 1141 CAGG //
Primer: HIVgp_R1 Sequence file: RDB12134_A5Dhd.seq
>04391_12134_A9FlF_HIVgp_R1_F02_06_ABI08.ab1 1 CAAACCCTTC TATTTTCCAC TGCTCTTTTT TCTCTCTGCA CCACTCTTCT CTTTGCCTTG 61 GTGGGTGCTA CTCCTAATGG TTCAATTTTT ACTACTTTAT ATTTATATAA TTCACTTCTC 121 CAATTGTCCC TCATATCTCC TCCTCCAGGT CTGAAGATCA GCGGCCGCTT GCTGTGCGGT 181 GGTCTTACTT TTGTTTTGCT CTTCCTCTAT CTTGTCTAAA GCTTCCTTGG TGTCTTTTAT 241 CTCTATCCTT TGATGCACAC AATAGAGGGT TGCTACTGTA TTATATAATG ATCTAAGTTC 301 TTCTGATCCT GTCTGAAGGG ATGGTTGTAG CTGTCCCAGT ATTTGTCTAC AGCCTTCTGA 361 TGTTTCTAAC AGGCCAGGAT TAACTGCGAA TCGTTCTAGC TCCCTGCTTG CCCATACTAT 421 ATGTTTTAAT TTATATTTTT TCTTTCCCCC TGGCCTTAAC CGAATTTTTT CCCATCGCGA 481 TCTAATTCTC CCCCGCTTAA TACTGACGCT CTCGCACCCA TCTCTCTCCT TCTAGCCTCC 541 GCTAGTCAAA ATTTTTGGCG TACTCACCAG TCGCCGCCCC TCGCCTCTTG CCGTGCGCGC 601 TTCAGCAAGC CGAGTCCTGC GTCGAGAGAG CTCCTCTGGT TTCCCTTTCG CTTTCAAGTC 661 CCTGTTCGGG CGCCACTGCT AGAGATTTTC CACACTGACT AAAAGGGTCT GAGGGATCTC 721 TAGTTACCAG AGTCACACAA CAGACGGGCA CACACTACTT GAAGCACTCA AGGCAAGCTT 781 TATTGAGGCT TAAGCAGTGG GTTCCCTAGT TAGCCAGAGA GCTCCCAGGC TCAGATCTGG 841 TCTAACCAGA GAGACCCAGT ACAGGCAAAA CGCGCTGCTT ATATAGACCT CCCACCGTAC 901 ACGCCTACCG CCCATTTGCG TCAATGGGGG CGGAGTTGTT ACGACATTTT GGAAAAGTCC 961 CGTTGATTTT GGTGCCCAAA ACAAACTCCC ATTTGACGTC AATGGGGGTG GGAGACTTGG 1021 AATCCCCGTG AGTCAAACCG GCTATCCACG CCCATTGATG TACTGCCAAA ACCGGCATCA 1081 ACCATGGTAA TAGCGGATGA ACTAATTACG TAGATGTACT GGCCAAGTAG GAAAGGTTCC 1141 ATAGGGTCAT GTACTGGGGG CATATTGTCA GGGGCGGGGT CT //
Primer: BGH_rev2 Sequence file: RDB12134_A5Dhe.seq
>04391_12134_A9FlF_BGH_rev2_E02_05_ABI08.ab1 1 GATTCAGGGT TAGGGAAGCA CGGGGGAGGG GCAACAACAG ATGGCTGGCA ACTAGAAGGC 61 ACAGTCGAGG CTGATCAGCG GGTTTAAACG GGCCCTGCTA GAGATTTTCC ACACTGACTA 121 AAAGGGTCTG AGGGATCTCT AGTTACCAGA GTCACACAAC AGACGGGCAC ACACTACTTG 181 AAGCACTCAA GGCAAGCTTT ATTGAGGCTT AAGCAGTGGG TTCCCTAGTT AGCCAGAGAG 241 CTCCCAGGCT CAGATCTGGT CTAACCAGAG AGACCCAGTA CAGTCCGGAT GCAGCTCTCG 301 GGCCATGTGA TGAAATGCTA GGCGGCTGTC AAACCTCCAC TCTAATACTT CTCTCTCCGG 361 GTCATCCATC CCATGCAGGC TCACAGGGTG TAACAAGCGG GTGTTCTCTC CTTCATTGGC 421 TTCTTCTACC TTCTCTTGCT CAACTGGTAC TAGCTTGTAG CACCATCCAA AGGTCAGTGG 481 ATATCTGATC CCTGGCCCTG GTGTGTAGTT CTGCCAATCA GGGAAGTAGC CTTGTGTGTG 541 GTAGATCCAC AGATCAAGGA TATCTTGTCT TCGTTGGGAG TGAATTAGCC CTTCCAGTCC 601 CCCCTTTTCT TTTAAAAAGT GGCTAAGATC TACAGCTGCC TTGTAAGTCA TTGGTCTTAA 661 AGGTACCTGA GGTGTGACTG GAAAACCCAC CTCCTCCTCC TCTTGTGCTT CTAGCCAGGC 721 ACAATCAGCA TTGGTAGCTG CTGTATTGCT ACTTGTGATT GCTCCATGTT TTTCTAGGTC 781 TCGACGGTAT CGATGCGGGG AGGCGGCCCA AAGGGAGATC CGACTCGTCT GAGGGCGAAG 841 GCGAAGACGC GGAAGAGGCC GCAGAGCCGG CAGCAGCCGC GGGAAGGAAG GTCCGCTGGA 901 TTGAGGGCCG AAGGGACGTA GCAGAAGGAC GTCCCGCGCA GAATCCAGGT GGCAACACAG 961 GCGAGCAGCC CATGGAAAGG ACGTCAGCTT CCCCGACACA CCACGGGAAT TTGTCAGTGC 1021 CCAACAGCCG AGCCCTGTCC AGCAGCCGGG CAGCAGGCGC GATGAGTTCG CGTTGCAATA 1081 GGAGGGGAAG CGAAGTTCCG AAAGAGCCTG AACGGTGATG CCAATGCCTC AATCATGGAT 1141 GCGTCAGTCA GATGTCG //
Primer: SV40pro_F_V2 Sequence file: RDB12134_A5Dhf.seq
>04391_12134_A9FlF_SV40pro_F_V2_G02_07_ABI08.ab1 1 GGTGAACCTT TTTTTGGAGG CCTAGGGCTT TTGCAAAAGC TCCCGGGGAG CTTGTATATC 61 CATTTTCGGA TCTGATCAGC ACGTGTTGAC AATTAATCAT CGGCATAGTA TATCGGCATA 121 GTATAATACG ACAAGGTGAG GAACTAAACC ATGGCCAAGT TGACCAGTGC CGTTCCGGTG 181 CTCACCGCGC GCGACGTCGC CGGAGCGGTC GAGTTCTGGA CCGACCGGCT CGGGTTCTCC 241 CGGGACTTCG TGGAGGACGA CTTCGCCGGT GTGGTCCGGG ACGACGTGAC CCTGTTCATC 301 AGCGCGGTCC AGGACCAGGT GGTGCCGGAC AACACCCTGG CCTGGGTGTG GGTGCGCGGC 361 CTGGACGAGC TGTACGCCGA GTGGTCGGAG GTCGTGTCCA CGAACTTCCG GGACGCCTCC 421 GGGCCGGCCA TGACCGAGAT CGGCGAGCAG CCGTGGGGGC GGGAGTTCGC CCTGCGCGAC 481 CCGGCCGGCA ACTGCGTGCA CTTCGTGGCC GAGGAGCAGG ACTGACACGT GCTACGAGAT 541 TTCGATTCCA CCGCCGCCTT CTATGAAAGG TTGGGCTTCG GAATCGTTTT CCGGGACGCC 601 GGCTGGATGA TCCTCCAGCG CGGGGATCTC ATGCTGGAGT TCTTCGCCCA CCCCAACTTG 661 TTTATTGCAG CTTATAATGG TTACAAATAA AGCAATAGCA TCACAAATTT CACAAATAAA 721 GCATTTTTTT CACTGCATTC TAGTTGTGGT TTGTCCAAAC TCATCAATGT ATCTTATCAT 781 GTCTGTATAC CGTCGACCTC TAGCTAGAGC TTGGCGTAAT CATGGTCATA GCTGTTTCCT 841 GTGTGAAATT GTTATCCGCT CACAATTCCA CACAACATAA CGAGCCGGAA GCATAAAGTG 901 TAAAAGCCTG GGGGTGCCTA ATGAGTGAAG CTAACTCACA TTAAATTGCG TTGCGCTCAC 961 TGCCCGCTTT CCAGTCGGGA AAACCCTGTC GTGCCAGCTG CATTTAATGA ATCGGCCCAA 1021 CGCGCGGGGA AGAAGGCGGG TTGCCGTAAT TGGGGCGGCT CTTCCGCTTT CCTCGGCTTC 1081 ACTTGAACTC GCTTGCGCCT CGGTTCGTTT CGGCTTGCCG ACGAGCGTAA TCCAGCTCAC 1141 CTCAAGGCGG TAAATACCGG GTTAATCCCC ACAGAAAAAT TCCAGG //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original	Miyoshi, H., Development of a self-inactivating lentivirus vector. J. Virol., 72 (10): 8150-8157 (1998). PMID 9733856.
user report	Kawasaki, N., TUFT1 interacts with RABGAP1 and regulates mTORC1 signaling Cell Discovery 4 (1): (2018). PMID 29423269.
user report	Yoshitomi, H., Human Sox4 facilitates the development of CXCL13-producing helper T cells in inflammatory environments. Nat. Commun. 9 (1): 3762 (2018). PMID 30232328.
user report	Ishibashi, M., CD200-positive cancer associated fibroblasts augment the sensitivity of Epidermal Growth Factor Receptor mutation-positive lung adenocarcinomas to EGFR Tyrosine kinase inhibitors. Sci. Rep. 7: 46662 (2017). PMID 28429795.
user report	Kobayashi, S., TGF-beta induces the differentiation of human CXCL13-producing CD4(+) T cells. Eur. J. Immunol. 46 (2): 360-371 (2016). PMID 26541894.
user report	Katsura, A., MicroRNA-31 is a positive modulator of endothelial-mesenchymal transition and associated secretory phenotype induced by TGF-beta. Genes Cells 21 (1): 99-116 PMID 26663584.
user report	Neri, S., Podoplanin-expressing cancer-associated fibroblasts lead and enhance the local invasion of cancer cells in lung adenocarcinoma. Int. J. Cancer. 137 (4): 784-796 (2015). PMID 25648219.
user report	Tanaka, Y., Dual Function of Histone H3 Lysine 36 Methyltransferase ASH1 in Regulation of Hox Gene Expression. PLoS One., 6 (11): e28171 (2011). PMID 22140534.
user report	Ohoka, N., The orphan nuclear receptor RORalpha restrains adipocyte differentiation through a reduction of C/EBPbeta activity and perilipin gene expression. Mol. Endocrinol., 23, 759-771 (2009). PMID 19324970.
user report	Naka, H., Requirement for COUP-TFI and II in the temporal specification of neural stem cells in CNS development. Nat. Neurosci. 11 (9): 1014-1023 (2008). PMID 19160499.
user report	Tsukamoto H., TCR ligand avidity determines the mode of B-Raf/Raf-1/ERK activation leading to the activation of human CD4+ T cell clone. Eur. J. Immunol., 36, 1926-1937 (2006). PMID 16791876.

2020.12.24

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