Resource data sheet
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CS-RfA-EG

Backbone vector of shRNA expressing lentivirus construct by Gateway(R) cloning with GFP marker driven by EF-1 alpha promoter.

Catalog number RDB04391
Resource name CS-RfA-EG
Clone info. Backbone vector of shRNA expressing lentivirus construct by Gateway(R) cloning with GFP marker driven by EF-1 alpha promoter.
Commonly requested with pENTR4-H1 (RDB04395) for generation of shRNA expression vector and with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394) for packaging. Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Vector backbone Plasmid
Selectable markers Please note that Zeo resistance marker is not included in the lentivirus produced from this vector. E. coli: Amp^r, Cm^r, ccdB.
Growth remarks Use DB3.1, ccdB Survival or equivalent to amplify this clone. 30 degC is fine.
Originator Miyoshi, Hiroyuki |
Lentiviral plasmid list, maps and sequences information. [link]

Distribution information

Terms and conditions for distribution The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe Dr. Hiroyuki Miyoshi as the Developer.
Ordering Please visit Ordering instruction. 
Order form [Credit Card Payment]  [Bank Transfer or Check Payment]
Material Transfer Agreement (MTA) [Word]
Remarks Remember that you will be working with samples containing infectious virus.
Use Survival2 (Invitrogen) or equivalent to amplify this clone.
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB04391 CS-RfA-EG DNA solution

Ordering instruction of plasmids [in Japanese] [in English]

References and tips

Electronic file

Electronic file Sequence RDB04391z.seq from Depositor
Electronic file Remarks, protocol and/or map (pdf) RDB04391.pdf from Depositor

Original reference

original Miyoshi, H., Development of a self-inactivating lentivirus vector. J. Virol., 72 (10): 8150-8157 (1998). PMID 9733856.

Further references such as user reports and related articles (go to bottom)

Featured content

Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB12134_A5Dhp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxEF_F1 | Sequence: RDB12134_A5Dha.seq
>04391_12134_A5Dh_pAxEF_F1_A01_01.ab1
    1 ATTTTTTTCT TCATTTCAGG TGTCGTGAAC ACGCTACCGG TCGCCACCAT GGTGAGCAAG
   61 GGCGAGGAGC TGTTCACCGG GGTGGTGCCC ATCCTGGTCG AGCTGGACGG CGACGTAAAC
  121 GGCCACAAGT TCAGCGTGTC CGGCGAGGGC GAGGGCGATG CCACCTACGG CAAGCTGACC
  181 CTGAAGTTCA TCTGCACCAC CGGCAAGCTG CCCGTGCCCT GGCCCACCCT CGTGACCACC
  241 CTGACCTACG GCGTGCAGTG CTTCAGCCGC TACCCCGACC ACATGAAGCA GCACGACTTC
  301 TTCAAGTCCG CCATGCCCGA AGGCTACGTC CAGGAGCGCA CCATCTTCTT CAAGGACGAC
  361 GGCAACTACA AGACCCGCGC CGAGGTGAAG TTCGAGGGCG ACACCCTGGT GAACCGCATC
  421 GAGCTGAAGG GCATCGACTT CAAGGAGGAC GGCAACATCC TGGGGCACAA GCTGGAGTAC
  481 AACTACAACA GCCACAACGT CTATATCATG GCCGACAAGC AGAAGAACGG CATCAAGGTG
  541 AACTTCAAGA TCCGCCACAA CATCGAGGAC GGCAGCGTGC AGCTCGCCGA CCACTACCAG
  601 CAGAACACCC CCATCGGCGA CGGCCCCGTG CTGCTGCCCG ACAACCACTA CCTGAGCACC
  661 CAGTCCGCCC TGAGCAAAGA CCCCAACGAG AAGCGCGATC ACATGGTCCT GCTGGAGTTC
  721 GTGACCGCCG CCGGGATCAC TCTCGGCATG GACGAGCTGT ACAAGTCCGG ACTCAGATCC
  781 TACTAGTAGG ATCTCGAGGG ATCAAGCTTA TCGATAATCA ACCTCTGGAT TACAAAATTT
  841 GTGAAAGATT GACTGGTATT CTTAACTATG TTGCTCCTTT TACGCTATGT GGATACGCTG
  901 CTTTAATGCC TTTGTATCAT GCTATTGCTT CCCGTATGGC TTTCATTTTC TCCTCCTTGT
  961 ATAAATCCTG GTTTGCTGTC TCTTTATGAG GAGTTGTGGC CCGTTGTCAG CAACGTGGCG
 1021 TGTGTGCACT GTGTTTGCTG ACGCAACCCC CCCACTGGAT TGGGGGCATT GCACACTGTC
 1081 AGCTCGTTTC CAGGAACTTC GCTTCCCTCC CTCCCTATTG ACTACGGCGG GAACTTCAAT
1141 TCGGCCCGG
//
Primer: Cm_M_R | Sequence: RDB12134_A5Dhb.seq
>04391_12134_A5Dh_Cm_M_R_B01_04.ab1
    1 CATGAGGTTA ACTATCCCAT ATCACCAGCT CACCGTCTTT CATTGCCATA CGGAATTCCG
   61 GATGAGCATT CATCAGGCGG GCAAGAATGT GAATAAAGGC CGGATAAAAC TTGTGCTTAT
  121 TTTTCTTTAC GGTCTTTAAA AAGGCCGTAA TATCCAGCTG AACGGTCTGG TTATAGGTAC
  181 ATTGAGCAAC TGACTGAAAT GCCTCAAAAT GTTCTTTACG ATGCCATTGG GATATATCAA
  241 CGGTGGTATA TCCAGTGATT TTTTTCTCCA TTTTAGCTTC CTTAGCTCCT GAAAATCTCG
  301 ACGGATCCTA ACTCAAAATC CACACATTAT ACGAGCCGGA AGCATAAAGT GTAAAGCCTG
  361 GGGTGCCTAA TGCGGCCGCC ATAGTGACTG GATATGTTGT GTTTTACAGT ATTATGTAGT
  421 CTGTTTTTTA TGCAAAATCT AATTTAATAT ATTGATATTT ATATCATTTT ACGTTTCTCG
  481 TTCAGCTTTT TTGTACAAAC TTGTGATAAT TCAAAGTGAA TTTCTGCTGT CCCTGTAATA
  541 AACCCGAAAA TTTTGAATTT TTGTAATTTG TTTTTGTAAT TCTTTAGTTT GTATGTCTGT
  601 TGCTATTATG TCTACTATTC TTTCCCCTGC ACTGTACCCC CCAATCCCCC CTTTTCTTTT
  661 AAAATTGTGG ATGAATACTG CCATTTGTAG ATCCGTTCAC TAATCGAATG GATCTGTCTC
  721 TGTCTCTCTC TCCACCTTCT TCTTCTATTC CTTCGGGCCT GTCGGGTCCC CTCGGGGTTG
  781 GGAGGTGGGT CTGAAACGAT AATGGTGAAT ATCCCTGCCT AACTCTATTC ACTATAGAAA
  841 GTACAGCAAA AACTATTCTT AAACCTACCA AGCCTCCTAC TATCATTATG AATAATTTTA
  901 TATACCACAG CCAATTTGTT ATGTTAAACC AATTCCACAA ACTTGCCCAT TTATCTAATT
  961 CCAATAATTC TTGTTCATTC TTTTCTTGCT GGTTTTGCGA TTCTTCAATT AAAGGAGTGT
 1021 ATTAAGCTTT GTGTAAATTG TTTAATTTCT CTGTCCCACT CCATCCCAGG GTCGTGTTGA
 1081 TTTCTAATCT GTTCCAGAGA TTTTATAACT CGACCTAGCC AATCTAGCAC AGCAGTTGGT
1141 GTGCCCAAAA TGGAGTTTTT TCCGGAGAGA GAC
//
Primer: EF1a-N_R | Sequence: RDB12134_A5Dhc.seq
>04391_12134_A5Dh_EF1a-N_R_C01_07.ab1
    1 CCTCTGGGGG ATGTGGGCGA TGTGCGCTCT GCCCACTGAC GGGCACCGGA GCCTCACGAA
   61 GCTTGAATTA TCACCACTTT GTACAAGAAA GCTGAACGAG AAACGTAAAA TGATATAAAT
  121 ATCAATATAT TAAATTAGAT TTTGCATAAA AAACAGACTA CATAATACTG TAAAACACAA
  181 CATATCCAGT CACTATGGTC GACCTGCAGA CTGGCTGTGT ATAAGGGAGC CTGACATTTA
  241 TATTCCCCAG AACATCAGGT TAATGGCGTT TTTGATGTCA TTTTCGCGGT GGCTGAGATC
  301 AGCCACTTCT TCCCCGATAA CGGAGACCGG CACACTGGCC ATATCGGTGG TCATCATGCG
  361 CCAGCTTTCA TCCCCGATAT GCACCACCGG GTAAAGTTCA CGGGAGACTT TATCTGACAG
  421 CAGACGTGCA CTGGCCAGGG GGATCACCAT CCGTCGCCCG GGCGTGTCAA TAATATCACT
  481 CTGTACATCC ACAAACAGAC GATAACGGCT CTCTCTTTTA TAGGTGTAAA CCTTAAACTG
  541 CATTTCACCA GCCCCTGTTC TCGTCAGCAA AAGAGCCGTT CATTTCAATA AACCGGGCGA
  601 CCTCAGCCAT CCCTTCCTGA TTTTCCGCTT TCCAGCGTTC GGCACGCAGA CGACGGGCTT
  661 CATTCTGCAT GGTTGTGCTT ACCAGACCGG AGATATTGAC ATCATATATG CCTTGAGCAA
  721 CTGATAGCTG TCGCTGTCAA CTGTCACTGT AATACGCTGC TTCATAGCAT ACCTCTTTTT
  781 GACATACTTC GGGTATACAT ATCAGTATAT ATTCTTATAC CGCAAAAATC AGCGCGCAAA
  841 TACGCATACT GTTATCTGGC TTTTAGTAAG CCGGATCCAC GCGTTTACGC CCCGCCCTGC
  901 CACTCATCGC AGTACTGTTG TAATTCATTA AGCATTCTGC CGACATGGAA AGCCATCACA
  961 AACGGCATGA TGAACCCTGA ATCGCCAGCG GCATCAGCAC CTTGTCGCCT TTGCGTATAT
 1021 ATTTGCCCAT GTGAAAACGG GGGCGAGAGT GTCCATATTG CCACGTTTAA TCAAACTGGT
 1081 GAACTCACCC AGGATGGCTG AACGAAACAT AATTCTCAAT AAACCTTTAG GAAAATAGGC
1141 CAGG
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Miyoshi, H., Development of a self-inactivating lentivirus vector. J. Virol., 72 (10): 8150-8157 (1998). PMID 9733856.
user report Kawasaki, N., TUFT1 interacts with RABGAP1 and regulates mTORC1 signaling Cell Discovery 4 (1): (2018). PMID 29423269.
user report Yoshitomi, H., Human Sox4 facilitates the development of CXCL13-producing helper T cells in inflammatory environments. Nat. Commun. 9 (1): 3762 (2018). PMID 30232328.
user report Ishibashi, M., CD200-positive cancer associated fibroblasts augment the sensitivity of Epidermal Growth Factor Receptor mutation-positive lung adenocarcinomas to EGFR Tyrosine kinase inhibitors. Sci. Rep. 7: 46662 (2017). PMID 28429795.
user report Kobayashi, S., TGF-beta induces the differentiation of human CXCL13-producing CD4(+) T cells. Eur. J. Immunol. 46 (2): 360-371 (2016). PMID 26541894.
user report Katsura, A., MicroRNA-31 is a positive modulator of endothelial-mesenchymal transition and associated secretory phenotype induced by TGF-beta. Genes Cells 21 (1): 99-116 PMID 26663584.
user report Neri, S., Podoplanin-expressing cancer-associated fibroblasts lead and enhance the local invasion of cancer cells in lung adenocarcinoma. Int. J. Cancer. 137 (4): 784-796 (2015). PMID 25648219.
user report Nishimoto, Y., Cellular toxicity induced by the 26-kDa fragment and amyotrophic lateral sclerosis-associated mutant forms of TAR DNA-binding protein 43 in human embryonic stem cell-derived motor neurons. Neurol. Clinical Neurosci., 1 (1): 24-31 (2013).
user report Ishimine, H., N-Cadherin is a prospective cell surface marker of human mesenchymal stem cells that have high ability for cardiomyocyte differentiation. Biochem Biophys Res Commun., 438 (4): 753-759 (2013). PMID 23899519.
user report Tanaka, Y., Dual Function of Histone H3 Lysine 36 Methyltransferase ASH1 in Regulation of Hox Gene Expression. PLoS One., 6 (11): e28171 (2011). PMID 22140534.
user report Ohoka, N., The orphan nuclear receptor RORalpha restrains adipocyte differentiation through a reduction of C/EBPbeta activity and perilipin gene expression. Mol. Endocrinol., 23, 759-771 (2009). PMID 19324970.
user report Naka, H., Requirement for COUP-TFI and II in the temporal specification of neural stem cells in CNS development. Nat. Neurosci. 11 (9): 1014-1023 (2008). PMID 19160499.
user report Tsukamoto H., TCR ligand avidity determines the mode of B-Raf/Raf-1/ERK activation leading to the activation of human CD4+ T cell clone. Eur. J. Immunol., 36, 1926-1937 (2006). PMID 16791876.

2019.10.05

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