Please review the QC test results indicated by icon below as well as clone information before placing your order.

CS-RfA-CG

Backbone vector plasmid of shRNA expressing lentivirus construct by Gateway(R) cloning with GFP marker driven by CMV promoter.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer.
Ordering	Please visit Ordering instruction.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA, for use for not-for-profit academic purpose)[Word] This RESOURCE contains a fluorescent protein owned or licensed by Global Life Sciences Solutions Operations UK Ltd. (Cytiva). Please refer to Schedule B and use Schedule A (download the Schedule A and Schedule B [link]).
Remarks	Remember that you will be working with samples containing infectious virus. Use Survival2 (Invitrogen) or equivalent to amplify this clone.

提供案内 (日本国内) [open/close]

提供条件	本件研究材料は、非営利機関の非営利学術研究に限って提供する。本件研究材料を利用した研究結果等を発表する際は、本件研究材料が故三好浩之博士により開発されたことを明示する。
提供依頼	手続きの詳細は、「レンチウイルスベクターの提供申し込み」をご覧ください。 提供依頼書 [Word] 提供同意書 (非営利機関による非営利学術研究用)[Word] GFPリソースを含みます。Schedule Bをご一読の上Schedule A (英語版のみ) の提出をお願いします （Schedule AとSchedule Bを入手[link])。 遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。
備考	このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。 このクローンの増殖にはSurvival2 (Invitrogen)あるいは同等の宿主を使用してください。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB12740_A5Hhp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: Cm_N_rev Region: CmR, attR1, cPPT_CTS, RRE Sequence file: RDB12740_A5Hha.seq
>04390_12740_A5Hh_Cm_N_rev_G01_19.ab1     1 CAGGTCTCAA TGTTCTTTAC GATGCCATTG GGATATATCA ACGGTGGTAT ATCCAGTGAT    61 TTTTTTCTCC ATTTTAGCTT CCTTAGCTCC TGAAAATCTC GACGGATCCT AACTCAAAAT   121 CCACACATTA TACGAGCCGG AAGCATAAAG TGTAAAGCCT GGGGTGCCTA ATGCGGCCGC   181 CATAGTGACT GGATATGTTG TGTTTTACAG TATTATGTAG TCTGTTTTTT ATGCAAAATC   241 TAATTTAATA TATTGATATT TATATCATTT TACGTTTCTC GTTCAGCTTT TTTGTACAAA   301 CTTGTGATAA TTCAAAGTGA ATTTCTGCTG TCCCTGTAAT AAACCCGAAA ATTTTGAATT   361 TTTGTAATTT GTTTTTGTAA TTCTTTAGTT TGTATGTCTG TTGCTATTAT GTCTACTATT   421 CTTTCCCCTG CACTGTACCC CCCAATCCCC CCTTTTCTTT TAAAATTGTG GATGAATACT   481 GCCATTTGTA GATCCGTTCA CTAATCGAAT GGATCTGTCT CTGTCTCTCT CTCCACCTTC   541 TTCTTCTATT CCTTCGGGCC TGTCGGGTCC CCTCGGGGTT GGGAGGTGGG TCTGAAACGA   601 TAATGGTGAA TATCCCTGCC TAACTCTATT CACTATAGAA AGTACAGCAA AAACTATTCT   661 TAAACCTACC AAGCCTCCTA CTATCATTAT GAATAATTTT ATATACCACA GCCAATTTGT   721 TATGTTAAAC CAATTCCACA AACTTGCCCA TTTATCTAAT TCCAATAATT CTTGTTCATT   781 CTTTTCTTGC TGGTTTTGCG ATTCTTCAAT TAAGGAGTGT ATTAAGCTTG TGTAATTGTT   841 AATTTCTCTG TCCCACTCCA TCCAGGTCGT GTGATTCCAA ATCTGTTCCA GAGATTTATT   901 ACTCCAACTA GCATTCCAAG GCACAGCAGT GGTGCAAATG AGTTTTCCAG AGCAACCCCA   961 AATCCCCAGG AGCTGTTGAT CCTTTAGGTA TCTTTCCACA GCCAGGGATT CTTGCCTGCA  1021 GCTGCCTTGG ATGCCCCAGA CTGTGAGTTG CACCAGAATG CTGGTGCGCT CATAGTCCTC 1081 AGCATTGATC TTGCTGCCTG CACTAATACC AGAACGATTA ATG //
Primer: ccdB-C_F Region: ccdB, attR2, CMV en 2, CMV pro 2 Sequence file: RDB12740_A5Hhb.seq
>04390_12740_A5Hh_ccdB-C_F_B01_04.ab1     1 CATCGGGGTA TGCATATCGG GGATGAAGCT GGCGCATGAT GACCACCGAT ATGGCCAGTG    61 TGCCGGTCTC CGTTATCGGG GAAGAAGTGG CTGATCTCAG CCACCGCGAA AATGACATCA   121 AAAACGCCAT TAACCTGATG TTCTGGGGAA TATAAATGTC AGGCTCCCTT ATACACAGCC   181 AGTCTGCAGG TCGACCATAG TGACTGGATA TGTTGTGTTT TACAGTATTA TGTAGTCTGT   241 TTTTTATGCA AAATCTAATT TAATATATTG ATATTTATAT CATTTTACGT TTCTCGTTCA   301 GCTTTCTTGT ACAAAGTGGT GATAATTCGA TTAATAGTAA TCAATTACGG GGTCATTAGT   361 TCATAGCCCA TATATGGAGT TCCGCGTTAC ATAACTTACG GTAAATGGCC CGCCTGGCTG   421 ACCGCCCAAC GACCCCCGCC CATTGACGTC AATAATGACG TATGTTCCCA TAGTAACGCC   481 AATAGGGACT TTCCATTGAC GTCAATGGGT GGAGTATTTA CGGTAAACTG CCCACTTGGC   541 AGTACATCAA GTGTATCATA TGCCAAGTAC GCCCCCTATT GACGTCAATG ACGGTAAATG   601 GCCCGCCTGG CATTATGCCC AGTACATGAC CTTATGGGAC TTTCCTACTT GGCAGTACAT   661 CTACGTATTA GTCATCGCTA TTACCATGGT GATGCGGTTT TGGCAGTACA TCAATGGGCG   721 TGGATAGCGG TTTGACTCAC GGGGATTTCC AAGTCTCCAC CCCATTGACG TCAATGGGAG   781 TTTGTTTTGG CACCAAAATC AACGGGACTT TCCAAAATGT CGTAACAACT CCGCCCCCAT   841 TGACGCAAAT GGGCGGTAGG CGTGTACGGT GGGAGGTCTA TATAAGCAGA GCTGGTTTAG   901 TGAACCGTCA GATCCGCTAG CGCTACCGGT CGCCACCATG GTGAGCAAGG GCGAGGAGCT   961 GTTCACCGGG GTGGTGCCCA TCCTGGTCGA GCTGGGACGG CGACGTAACG GGCCACAAGT  1021 TTCAGCGGTG TTCCGGCGGA GGCGAGGGCG AATGGCTACT AACGGCAAGC CTGACCTGAG 1081 TCATCCTGCA CTAATCTGGC CAAGGCTGGA CTCTCGGTTG C //
Primer: CSII-CMV5' Region: EGFP, WPRE Sequence file: RDB12740_A5Hhc.seq
>04390_12740_A5Hh_CSII-CMV5'_C01_07.ab1     1 AATGCAACGC TCGGTCGCCA CCATGGTGAG CAAGGGCGAG GAGCTGTTCA CCGGGGTGGT    61 GCCCATCCTG GTCGAGCTGG ACGGCGACGT AAACGGCCAC AAGTTCAGCG TGTCCGGCGA   121 GGGCGAGGGC GATGCCACCT ACGGCAAGCT GACCCTGAAG TTCATCTGCA CCACCGGCAA   181 GCTGCCCGTG CCCTGGCCCA CCCTCGTGAC CACCCTGACC TACGGCGTGC AGTGCTTCAG   241 CCGCTACCCC GACCACATGA AGCAGCACGA CTTCTTCAAG TCCGCCATGC CCGAAGGCTA   301 CGTCCAGGAG CGCACCATCT TCTTCAAGGA CGACGGCAAC TACAAGACCC GCGCCGAGGT   361 GAAGTTCGAG GGCGACACCC TGGTGAACCG CATCGAGCTG AAGGGCATCG ACTTCAAGGA   421 GGACGGCAAC ATCCTGGGGC ACAAGCTGGA GTACAACTAC AACAGCCACA ACGTCTATAT   481 CATGGCCGAC AAGCAGAAGA ACGGCATCAA GGTGAACTTC AAGATCCGCC ACAACATCGA   541 GGACGGCAGC GTGCAGCTCG CCGACCACTA CCAGCAGAAC ACCCCCATCG GCGACGGCCC   601 CGTGCTGCTG CCCGACAACC ACTACCTGAG CACCCAGTCC GCCCTGAGCA AAGACCCCAA   661 CGAGAAGCGC GATCACATGG TCCTGCTGGA GTTCGTGACC GCCGCCGGGA TCACTCTCGG   721 CATGGACGAG CTGTACAAGT CCGGACTCAG ATCCTACTAG TAGGATCTCG AGGGATCAAG   781 CTTATCGATA ATCAACCTCT GGATTACAAA ATTTGTGAAA GATTGACTGG TATTCCTTAA   841 CTATGTTGCT CCTTTTACGC TATGTGGATA CGCTGCTTTA ATGCCCTTTG TATCATGCTA   901 TTGCTCCCGT ATGGCTTCCA TTTTCCTCCC TCCTGTTAAA TCCTGATGCT GGTCTCTTTA   961 TGAAGGAGTG TGCCGTGTCA GCACGTGGCG TGGTGTGCCA CTGGATGCTG AACGCAACCC 1021 CCACTGGATG GCAATGCCAC ATTCAGTCCT ATCGGAACCT CGCTTCCCTC TATCCA //
Primer: HIVgp_R1 Region: HIV-1 psi, 5' LTR, CMV pro 1 Sequence file: RDB12740_A5Hhd.seq
>04390_12740_A5Hh_HIVgp-R1_D01_10.ab1     1 CAGCTCTATT CCACTGCTCT TTTTTCTCTC TGCACCACTC TTCTCTTTGC CTTGGTGGGT    61 GCTACTCCTA ATGGTTCAAT TTTTACTACT TTATATTTAT ATAATTCACT TCTCCAATTG   121 TCCCTCATAT CTCCTCCTCC AGGTCTGAAG ATCAGCGGCC GCTTGCTGTG CGGTGGTCTT   181 ACTTTTGTTT TGCTCTTCCT CTATCTTGTC TAAAGCTTCC TTGGTGTCTT TTATCTCTAT   241 CCTTTGATGC ACACAATAGA GGGTTGCTAC TGTATTATAT AATGATCTAA GTTCTTCTGA   301 TCCTGTCTGA AGGGATGGTT GTAGCTGTCC CAGTATTTGT CTACAGCCTT CTGATGTTTC   361 TAACAGGCCA GGATTAACTG CGAATCGTTC TAGCTCCCTG CTTGCCCATA CTATATGTTT   421 TAATTTATAT TTTTTCTTTC CCCCTGGCCT TAACCGAATT TTTTCCCATC GCGATCTAAT   481 TCTCCCCCGC TTAATACTGA CGCTCTCGCA CCCATCTCTC TCCTTCTAGC CTCCGCTAGT   541 CAAAATTTTT GGCGTACTCA CCAGTCGCCG CCCCTCGCCT CTTGCCGTGC GCGCTTCAGC   601 AAGCCGAGTC CTGCGTCGAG AGAGCTCCTC TGGTTTCCCT TTCGCTTTCA AGTCCCTGTT   661 CGGGCGCCAC TGCTAGAGAT TTTCCACACT GACTAAAAGG GTCTGAGGGA TCTCTAGTTA   721 CCAGAGTCAC ACAACAGACG GGCACACACT ACTTGAAGCA CTCAAAGGCA AGCTTTATTG   781 AGGCTTAAGC AGTGGGTTCC CTAGTTAGCC AGAGAGCTCC CAGGCTCAGA TCTGGTCTAA   841 CCAGAGAGAC CCAGTACAGG CAAAACGCGC TGCTTATATA GACCTCCCCA CCGTACACGC   901 CTACCGCCCA TTTTGCGTCA ATGGGGCGAG TTGTTACGAC ATTTTGGAAA GGTCCCGTTG   961 ATTTTGTGCC AAAAACAAAC TCCCATTGAC GTCAATGGGG TTGCAGACTG GAATCCCCGT  1021 GGAGTTCATC GGCTAATCCA CGCCCCCATG ATGTACTGCC AATCGCATCA GCATGATAAT 1081 AGC //
Primer: BGH_rev Region: 3' LTR, WPRE Sequence file: RDB12740_A5Hhe.seq
>04390_12740_A5Hh_BGHrev_E01_13.ab1     1 GCGTCGGGGG TCTGCTAGAG ATTTTCCACA CTGACTAAAA GGGTCTGAGG GATCTCTAGT    61 TACCAGAGTC ACACAACAGA CGGGCACACA CTACTTGAAG CACTCAAGGC AAGCTTTATT   121 GAGGCTTAAG CAGTGGGTTC CCTAGTTAGC CAGAGAGCTC CCAGGCTCAG ATCTGGTCTA   181 ACCAGAGAGA CCCAGTACAG TCCGGATGCA GCTCTCGGGC CATGTGATGA AATGCTAGGC   241 GGCTGTCAAA CCTCCACTCT AATACTTCTC TCTCCGGGTC ATCCATCCCA TGCAGGCTCA   301 CAGGGTGTAA CAAGCGGGTG TTCTCTCCTT CATTGGCTTC TTCTACCTTC TCTTGCTCAA   361 CTGGTACTAG CTTGTAGCAC CATCCAAAGG TCAGTGGATA TCTGATCCCT GGCCCTGGTG   421 TGTAGTTCTG CCAATCAGGG AAGTAGCCTT GTGTGTGGTA GATCCACAGA TCAAGGATAT   481 CTTGTCTTCG TTGGGAGTGA ATTAGCCCTT CCAGTCCCCC CTTTTCTTTT AAAAAGTGGC   541 TAAGATCTAC AGCTGCCTTG TAAGTCATTG GTCTTAAAGG TACCTGAGGT GTGACTGGAA   601 AACCCACCTC CTCCTCCTCT TGTGCTTCTA GCCAGGCACA ATCAGCATTG GTAGCTGCTG   661 TATTGCTACT TGTGATTGCT CCATGTTTTT CTAGGTCTCG ACGGTATCGA TGCGGGGAGG   721 CGGCCCAAAG GGAGATCCGA CTCGTCTGAG GGCGAAGGCG AAGACGCGGA AGAGGCCGCA   781 GAGCCGGCAG CAGGCCGCGG GAAGGAAGGT CCGCTGGATT GAGGGCCGAA AGGGACGTAG   841 CAGAAGGACG TCCCGCGCAG AATCCAGGTG GCAACACAGG CGAGCAGCCA TGGGAAAGGA   901 CGTCAGCTTC CCCGACAACA CCACGGGAAA TTGTCAGTGC TCAACAGCCG AGCTCCTGTC   961 CAGCAGCGGG CAAGCAGCGG CGATGAGTTC CGCCGTGCAT AGGAGGGGGA AGCGAAGTCC  1021 CCGGAAGGAG CTGACAGTTG TGCATGCCAA CAATTGGGGA TGCGTCAGCA CCCATGCAAC 1081 AGTCAGTGCT GACACGGGTC C //
Primer: SV40pro_F Region: SV40 pro_ori, Zeo, SV40 pA Sequence file: RDB12740_A5Hhf.seq
>04390_12740_A5Hh_SV40pro_F_F01_16.ab1     1 ACTGGTCGGG GGACATCAGA GTAGTGAGGA GGCTTTTTTG GAGGCCTAGG CTTTTGCAAA    61 AAGCTCCCGG GAGCTTGTAT ATCCATTTTC GGATCTGATC AGCACGTGTT GACAATTAAT   121 CATCGGCATA GTATATCGGC ATAGTATAAT ACGACAAGGT GAGGAACTAA ACCATGGCCA   181 AGTTGACCAG TGCCGTTCCG GTGCTCACCG CGCGCGACGT CGCCGGAGCG GTCGAGTTCT   241 GGACCGACCG GCTCGGGTTC TCCCGGGACT TCGTGGAGGA CGACTTCGCC GGTGTGGTCC   301 GGGACGACGT GACCCTGTTC ATCAGCGCGG TCCAGGACCA GGTGGTGCCG GACAACACCC   361 TGGCCTGGGT GTGGGTGCGC GGCCTGGACG AGCTGTACGC CGAGTGGTCG GAGGTCGTGT   421 CCACGAACTT CCGGGACGCC TCCGGGCCGG CCATGACCGA GATCGGCGAG CAGCCGTGGG   481 GGCGGGAGTT CGCCCTGCGC GACCCGGCCG GCAACTGCGT GCACTTCGTG GCCGAGGAGC   541 AGGACTGACA CGTGCTACGA GATTTCGATT CCACCGCCGC CTTCTATGAA AGGTTGGGCT   601 TCGGAATCGT TTTCCGGGAC GCCGGCTGGA TGATCCTCCA GCGCGGGGAT CTCATGCTGG   661 AGTTCTTCGC CCACCCCAAC TTGTTTATTG CAGCTTATAA TGGTTACAAA TAAAGCAATA   721 GCATCACAAA TTTCACAAAT AAAGCATTTT TTTCACTGCA TTCTAGTTGT GGTTTGTCCA   781 AACTCATCAA TGTATCTTAT CATGTCTGTA TACCGTCGAC CTCTAGCTAG AGCTTGGCGT   841 AATCATGGTC ATAGCTGTTT CCTGTGTGAA ATTGTTATCC GCTCACAATT CCACACAACA   901 TACGAGCCGG AAGCATAAAG TGTAAAGCCT GGGGTGCCTA ATGAGTGAGC TAACTCACAT   961 TAATTGCGTT GCGCTCACTG CCCGGCTTTC CCAGTCGGGA AACCCTGTCG TGCCCAGCCT  1021 GCATTAAATG AAATCGGCCA ACGCGCGGGG AGAGCGATTT GCGTAATTGG GCGCTCATCC 1081 GCATCCTTCG CTCAACTGAC TCCGCCTTGG GCGACAC //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2022.07.13

GNP_filter3_RDBDEP_html_220713.pl