Resource data sheet
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CS-RfA-CG

Shuttle vector to generate recombinant Lentiviral vector expressing GFP by Gateway(R) system.

Catalog number RDB04390
Resource name CS-RfA-CG
Clone info. Shuttle vector to generate recombinant Lentiviral vector. Gateway(R) destination vector with GFP as a marker.
Use DB3.1 (Invitrogen) or equivalent to amplify this clone. Commonly requested with pENTR4-H1 (RDB04395) for generation of shRNA expression vector and with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394) for packaging.
Vector backbone Plasmid
Selectable markers Amp^r
Originator Miyoshi, Hiroyuki |

Distribution information

CLICK here for plasmid list and map. [link]
Forms for plasmid request [in Japanese] [in English]
Terms and conditions for distribution The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose.
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Remarks ((Remarks)) Remember that you will be working with samples containing infectious virus.
(Japanese text [open/close])

Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB12740_A5Hhp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: Cm_N_rev RDB12740_A5Hha.seq
>04390_12740_A5Hh_Cm_N_rev_G01_19.ab1
    1 CAGGTCTCAA TGTTCTTTAC GATGCCATTG GGATATATCA ACGGTGGTAT ATCCAGTGAT
   61 TTTTTTCTCC ATTTTAGCTT CCTTAGCTCC TGAAAATCTC GACGGATCCT AACTCAAAAT
  121 CCACACATTA TACGAGCCGG AAGCATAAAG TGTAAAGCCT GGGGTGCCTA ATGCGGCCGC
  181 CATAGTGACT GGATATGTTG TGTTTTACAG TATTATGTAG TCTGTTTTTT ATGCAAAATC
  241 TAATTTAATA TATTGATATT TATATCATTT TACGTTTCTC GTTCAGCTTT TTTGTACAAA
  301 CTTGTGATAA TTCAAAGTGA ATTTCTGCTG TCCCTGTAAT AAACCCGAAA ATTTTGAATT
  361 TTTGTAATTT GTTTTTGTAA TTCTTTAGTT TGTATGTCTG TTGCTATTAT GTCTACTATT
  421 CTTTCCCCTG CACTGTACCC CCCAATCCCC CCTTTTCTTT TAAAATTGTG GATGAATACT
  481 GCCATTTGTA GATCCGTTCA CTAATCGAAT GGATCTGTCT CTGTCTCTCT CTCCACCTTC
  541 TTCTTCTATT CCTTCGGGCC TGTCGGGTCC CCTCGGGGTT GGGAGGTGGG TCTGAAACGA
  601 TAATGGTGAA TATCCCTGCC TAACTCTATT CACTATAGAA AGTACAGCAA AAACTATTCT
  661 TAAACCTACC AAGCCTCCTA CTATCATTAT GAATAATTTT ATATACCACA GCCAATTTGT
  721 TATGTTAAAC CAATTCCACA AACTTGCCCA TTTATCTAAT TCCAATAATT CTTGTTCATT
  781 CTTTTCTTGC TGGTTTTGCG ATTCTTCAAT TAAGGAGTGT ATTAAGCTTG TGTAATTGTT
  841 AATTTCTCTG TCCCACTCCA TCCAGGTCGT GTGATTCCAA ATCTGTTCCA GAGATTTATT
  901 ACTCCAACTA GCATTCCAAG GCACAGCAGT GGTGCAAATG AGTTTTCCAG AGCAACCCCA
  961 AATCCCCAGG AGCTGTTGAT CCTTTAGGTA TCTTTCCACA GCCAGGGATT CTTGCCTGCA
 1021 GCTGCCTTGG ATGCCCCAGA CTGTGAGTTG CACCAGAATG CTGGTGCGCT CATAGTCCTC
1081 AGCATTGATC TTGCTGCCTG CACTAATACC AGAACGATTA ATG
//
Primer: ccdB-C_F RDB12740_A5Hhb.seq
>04390_12740_A5Hh_ccdB-C_F_B01_04.ab1
    1 CATCGGGGTA TGCATATCGG GGATGAAGCT GGCGCATGAT GACCACCGAT ATGGCCAGTG
   61 TGCCGGTCTC CGTTATCGGG GAAGAAGTGG CTGATCTCAG CCACCGCGAA AATGACATCA
  121 AAAACGCCAT TAACCTGATG TTCTGGGGAA TATAAATGTC AGGCTCCCTT ATACACAGCC
  181 AGTCTGCAGG TCGACCATAG TGACTGGATA TGTTGTGTTT TACAGTATTA TGTAGTCTGT
  241 TTTTTATGCA AAATCTAATT TAATATATTG ATATTTATAT CATTTTACGT TTCTCGTTCA
  301 GCTTTCTTGT ACAAAGTGGT GATAATTCGA TTAATAGTAA TCAATTACGG GGTCATTAGT
  361 TCATAGCCCA TATATGGAGT TCCGCGTTAC ATAACTTACG GTAAATGGCC CGCCTGGCTG
  421 ACCGCCCAAC GACCCCCGCC CATTGACGTC AATAATGACG TATGTTCCCA TAGTAACGCC
  481 AATAGGGACT TTCCATTGAC GTCAATGGGT GGAGTATTTA CGGTAAACTG CCCACTTGGC
  541 AGTACATCAA GTGTATCATA TGCCAAGTAC GCCCCCTATT GACGTCAATG ACGGTAAATG
  601 GCCCGCCTGG CATTATGCCC AGTACATGAC CTTATGGGAC TTTCCTACTT GGCAGTACAT
  661 CTACGTATTA GTCATCGCTA TTACCATGGT GATGCGGTTT TGGCAGTACA TCAATGGGCG
  721 TGGATAGCGG TTTGACTCAC GGGGATTTCC AAGTCTCCAC CCCATTGACG TCAATGGGAG
  781 TTTGTTTTGG CACCAAAATC AACGGGACTT TCCAAAATGT CGTAACAACT CCGCCCCCAT
  841 TGACGCAAAT GGGCGGTAGG CGTGTACGGT GGGAGGTCTA TATAAGCAGA GCTGGTTTAG
  901 TGAACCGTCA GATCCGCTAG CGCTACCGGT CGCCACCATG GTGAGCAAGG GCGAGGAGCT
  961 GTTCACCGGG GTGGTGCCCA TCCTGGTCGA GCTGGGACGG CGACGTAACG GGCCACAAGT
 1021 TTCAGCGGTG TTCCGGCGGA GGCGAGGGCG AATGGCTACT AACGGCAAGC CTGACCTGAG
1081 TCATCCTGCA CTAATCTGGC CAAGGCTGGA CTCTCGGTTG C
//
Primer: CSII-CMV5' RDB12740_A5Hhc.seq
>04390_12740_A5Hh_CSII-CMV5'_C01_07.ab1
    1 AATGCAACGC TCGGTCGCCA CCATGGTGAG CAAGGGCGAG GAGCTGTTCA CCGGGGTGGT
   61 GCCCATCCTG GTCGAGCTGG ACGGCGACGT AAACGGCCAC AAGTTCAGCG TGTCCGGCGA
  121 GGGCGAGGGC GATGCCACCT ACGGCAAGCT GACCCTGAAG TTCATCTGCA CCACCGGCAA
  181 GCTGCCCGTG CCCTGGCCCA CCCTCGTGAC CACCCTGACC TACGGCGTGC AGTGCTTCAG
  241 CCGCTACCCC GACCACATGA AGCAGCACGA CTTCTTCAAG TCCGCCATGC CCGAAGGCTA
  301 CGTCCAGGAG CGCACCATCT TCTTCAAGGA CGACGGCAAC TACAAGACCC GCGCCGAGGT
  361 GAAGTTCGAG GGCGACACCC TGGTGAACCG CATCGAGCTG AAGGGCATCG ACTTCAAGGA
  421 GGACGGCAAC ATCCTGGGGC ACAAGCTGGA GTACAACTAC AACAGCCACA ACGTCTATAT
  481 CATGGCCGAC AAGCAGAAGA ACGGCATCAA GGTGAACTTC AAGATCCGCC ACAACATCGA
  541 GGACGGCAGC GTGCAGCTCG CCGACCACTA CCAGCAGAAC ACCCCCATCG GCGACGGCCC
  601 CGTGCTGCTG CCCGACAACC ACTACCTGAG CACCCAGTCC GCCCTGAGCA AAGACCCCAA
  661 CGAGAAGCGC GATCACATGG TCCTGCTGGA GTTCGTGACC GCCGCCGGGA TCACTCTCGG
  721 CATGGACGAG CTGTACAAGT CCGGACTCAG ATCCTACTAG TAGGATCTCG AGGGATCAAG
  781 CTTATCGATA ATCAACCTCT GGATTACAAA ATTTGTGAAA GATTGACTGG TATTCCTTAA
  841 CTATGTTGCT CCTTTTACGC TATGTGGATA CGCTGCTTTA ATGCCCTTTG TATCATGCTA
  901 TTGCTCCCGT ATGGCTTCCA TTTTCCTCCC TCCTGTTAAA TCCTGATGCT GGTCTCTTTA
  961 TGAAGGAGTG TGCCGTGTCA GCACGTGGCG TGGTGTGCCA CTGGATGCTG AACGCAACCC
1021 CCACTGGATG GCAATGCCAC ATTCAGTCCT ATCGGAACCT CGCTTCCCTC TATCCA
//
Primer: HIVgp_R1 RDB12740_A5Hhd.seq
>04390_12740_A5Hh_HIVgp-R1_D01_10.ab1
    1 CAGCTCTATT CCACTGCTCT TTTTTCTCTC TGCACCACTC TTCTCTTTGC CTTGGTGGGT
   61 GCTACTCCTA ATGGTTCAAT TTTTACTACT TTATATTTAT ATAATTCACT TCTCCAATTG
  121 TCCCTCATAT CTCCTCCTCC AGGTCTGAAG ATCAGCGGCC GCTTGCTGTG CGGTGGTCTT
  181 ACTTTTGTTT TGCTCTTCCT CTATCTTGTC TAAAGCTTCC TTGGTGTCTT TTATCTCTAT
  241 CCTTTGATGC ACACAATAGA GGGTTGCTAC TGTATTATAT AATGATCTAA GTTCTTCTGA
  301 TCCTGTCTGA AGGGATGGTT GTAGCTGTCC CAGTATTTGT CTACAGCCTT CTGATGTTTC
  361 TAACAGGCCA GGATTAACTG CGAATCGTTC TAGCTCCCTG CTTGCCCATA CTATATGTTT
  421 TAATTTATAT TTTTTCTTTC CCCCTGGCCT TAACCGAATT TTTTCCCATC GCGATCTAAT
  481 TCTCCCCCGC TTAATACTGA CGCTCTCGCA CCCATCTCTC TCCTTCTAGC CTCCGCTAGT
  541 CAAAATTTTT GGCGTACTCA CCAGTCGCCG CCCCTCGCCT CTTGCCGTGC GCGCTTCAGC
  601 AAGCCGAGTC CTGCGTCGAG AGAGCTCCTC TGGTTTCCCT TTCGCTTTCA AGTCCCTGTT
  661 CGGGCGCCAC TGCTAGAGAT TTTCCACACT GACTAAAAGG GTCTGAGGGA TCTCTAGTTA
  721 CCAGAGTCAC ACAACAGACG GGCACACACT ACTTGAAGCA CTCAAAGGCA AGCTTTATTG
  781 AGGCTTAAGC AGTGGGTTCC CTAGTTAGCC AGAGAGCTCC CAGGCTCAGA TCTGGTCTAA
  841 CCAGAGAGAC CCAGTACAGG CAAAACGCGC TGCTTATATA GACCTCCCCA CCGTACACGC
  901 CTACCGCCCA TTTTGCGTCA ATGGGGCGAG TTGTTACGAC ATTTTGGAAA GGTCCCGTTG
  961 ATTTTGTGCC AAAAACAAAC TCCCATTGAC GTCAATGGGG TTGCAGACTG GAATCCCCGT
 1021 GGAGTTCATC GGCTAATCCA CGCCCCCATG ATGTACTGCC AATCGCATCA GCATGATAAT
1081 AGC
//
Primer: BGH_rev RDB12740_A5Hhe.seq
>04390_12740_A5Hh_BGHrev_E01_13.ab1
    1 GCGTCGGGGG TCTGCTAGAG ATTTTCCACA CTGACTAAAA GGGTCTGAGG GATCTCTAGT
   61 TACCAGAGTC ACACAACAGA CGGGCACACA CTACTTGAAG CACTCAAGGC AAGCTTTATT
  121 GAGGCTTAAG CAGTGGGTTC CCTAGTTAGC CAGAGAGCTC CCAGGCTCAG ATCTGGTCTA
  181 ACCAGAGAGA CCCAGTACAG TCCGGATGCA GCTCTCGGGC CATGTGATGA AATGCTAGGC
  241 GGCTGTCAAA CCTCCACTCT AATACTTCTC TCTCCGGGTC ATCCATCCCA TGCAGGCTCA
  301 CAGGGTGTAA CAAGCGGGTG TTCTCTCCTT CATTGGCTTC TTCTACCTTC TCTTGCTCAA
  361 CTGGTACTAG CTTGTAGCAC CATCCAAAGG TCAGTGGATA TCTGATCCCT GGCCCTGGTG
  421 TGTAGTTCTG CCAATCAGGG AAGTAGCCTT GTGTGTGGTA GATCCACAGA TCAAGGATAT
  481 CTTGTCTTCG TTGGGAGTGA ATTAGCCCTT CCAGTCCCCC CTTTTCTTTT AAAAAGTGGC
  541 TAAGATCTAC AGCTGCCTTG TAAGTCATTG GTCTTAAAGG TACCTGAGGT GTGACTGGAA
  601 AACCCACCTC CTCCTCCTCT TGTGCTTCTA GCCAGGCACA ATCAGCATTG GTAGCTGCTG
  661 TATTGCTACT TGTGATTGCT CCATGTTTTT CTAGGTCTCG ACGGTATCGA TGCGGGGAGG
  721 CGGCCCAAAG GGAGATCCGA CTCGTCTGAG GGCGAAGGCG AAGACGCGGA AGAGGCCGCA
  781 GAGCCGGCAG CAGGCCGCGG GAAGGAAGGT CCGCTGGATT GAGGGCCGAA AGGGACGTAG
  841 CAGAAGGACG TCCCGCGCAG AATCCAGGTG GCAACACAGG CGAGCAGCCA TGGGAAAGGA
  901 CGTCAGCTTC CCCGACAACA CCACGGGAAA TTGTCAGTGC TCAACAGCCG AGCTCCTGTC
  961 CAGCAGCGGG CAAGCAGCGG CGATGAGTTC CGCCGTGCAT AGGAGGGGGA AGCGAAGTCC
 1021 CCGGAAGGAG CTGACAGTTG TGCATGCCAA CAATTGGGGA TGCGTCAGCA CCCATGCAAC
1081 AGTCAGTGCT GACACGGGTC C
//
Primer: SV40pro_F RDB12740_A5Hhf.seq
>04390_12740_A5Hh_SV40pro_F_F01_16.ab1
    1 ACTGGTCGGG GGACATCAGA GTAGTGAGGA GGCTTTTTTG GAGGCCTAGG CTTTTGCAAA
   61 AAGCTCCCGG GAGCTTGTAT ATCCATTTTC GGATCTGATC AGCACGTGTT GACAATTAAT
  121 CATCGGCATA GTATATCGGC ATAGTATAAT ACGACAAGGT GAGGAACTAA ACCATGGCCA
  181 AGTTGACCAG TGCCGTTCCG GTGCTCACCG CGCGCGACGT CGCCGGAGCG GTCGAGTTCT
  241 GGACCGACCG GCTCGGGTTC TCCCGGGACT TCGTGGAGGA CGACTTCGCC GGTGTGGTCC
  301 GGGACGACGT GACCCTGTTC ATCAGCGCGG TCCAGGACCA GGTGGTGCCG GACAACACCC
  361 TGGCCTGGGT GTGGGTGCGC GGCCTGGACG AGCTGTACGC CGAGTGGTCG GAGGTCGTGT
  421 CCACGAACTT CCGGGACGCC TCCGGGCCGG CCATGACCGA GATCGGCGAG CAGCCGTGGG
  481 GGCGGGAGTT CGCCCTGCGC GACCCGGCCG GCAACTGCGT GCACTTCGTG GCCGAGGAGC
  541 AGGACTGACA CGTGCTACGA GATTTCGATT CCACCGCCGC CTTCTATGAA AGGTTGGGCT
  601 TCGGAATCGT TTTCCGGGAC GCCGGCTGGA TGATCCTCCA GCGCGGGGAT CTCATGCTGG
  661 AGTTCTTCGC CCACCCCAAC TTGTTTATTG CAGCTTATAA TGGTTACAAA TAAAGCAATA
  721 GCATCACAAA TTTCACAAAT AAAGCATTTT TTTCACTGCA TTCTAGTTGT GGTTTGTCCA
  781 AACTCATCAA TGTATCTTAT CATGTCTGTA TACCGTCGAC CTCTAGCTAG AGCTTGGCGT
  841 AATCATGGTC ATAGCTGTTT CCTGTGTGAA ATTGTTATCC GCTCACAATT CCACACAACA
  901 TACGAGCCGG AAGCATAAAG TGTAAAGCCT GGGGTGCCTA ATGAGTGAGC TAACTCACAT
  961 TAATTGCGTT GCGCTCACTG CCCGGCTTTC CCAGTCGGGA AACCCTGTCG TGCCCAGCCT
 1021 GCATTAAATG AAATCGGCCA ACGCGCGGGG AGAGCGATTT GCGTAATTGG GCGCTCATCC
1081 GCATCCTTCG CTCAACTGAC TCCGCCTTGG GCGACAC
//

Please visit plasmidSequencing and PCR primers for primer information.

References and tips

Electronic file

References

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user report Koyama-Nasu, R, The cancer stem cell marker CD133 interacts with plakoglobin and controls desmoglein-2 protein levels. PLoS One, 8 (1): e53710 (2013). PMID 23326490.
user report Yanagida, S., ASBEL, an ANA/BTG3 antisense transcript required for tumorigenicity of ovarian carcinoma. Sci. Rep. 2013 Feb 19;3:1305 PMID 23419616.
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2017.10.23

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