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CS-RfA-CG (#RDB04390)

Backbone vector plasmid of shRNA expressing lentivirus construct by Gateway(R) cloning with GFP marker driven by CMV promoter.


Drawn by SnapGene® software
Sequence information
(Assembled from experimentally sequenced data)		 GenBank Flat File Format open
SnapGene file download
Publication Unpublished bioresource
Test sheet Data Sheet open 
 
Clone info. Backbone vector plasmid of shRNA expressing lentivirus construct by Gateway(R) cloning with GFP marker driven by CMV promoter.
Comment Commonly requested with pENTR4-H1 (RDB04395) for generation of shRNA expression vector and with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394) for packaging.
Vector backbone Plasmid
Selectable markers Ampicillin, Chloramphenicol, ccdB (E. coli). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Growth conditions 37C, LB+Amp
Growth remarks Use DB3.1, ccdB Survival or equivalent to amplify this clone. 30 degC is fine.
Depositor|Developer Miyoshi, Hiroyuki |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Ordering instruction.[link] 
Terms and conditions for distribution The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer.
Remarks Remember that you will be working with samples containing infectious virus.
Use Survival2 (Invitrogen) or equivalent to amplify this clone.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「レンチウイルスベクターの提供申し込み」をご覧ください。
MTAに書く使用条件 本件研究材料は、非営利機関の非営利学術研究に限って提供する。本件研究材料を利用した研究結果等を発表する際は、本件研究材料が故三好浩之博士により開発されたことを明示する。
備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。
このクローンの増殖にはSurvival2 (Invitrogen)あるいは同等の宿主を使用してください。

Catalog # Resource name Shipping form Fee
RDB04390 CS-RfA-CG DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Ordering instruction of plasmids [in Japanese] [in English]

How to cite this biological resource

Materials & Methods section:

The CS-RfA-CG was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB04390).

Reference section:

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

user_report Watanabe, H., Astrocytic APOE4 genotype-mediated negative impacts on synaptic architecture in human pluripotent stem cell model. Stem Cell Reports 18 (9): 1854-1869 (2023). PMID 37657448. [PubMed] [Article] [RRC of NBRP]
user_report Shimizu, K., Diurnal variation in declarative memory and the involvement of SCOP in cognitive functions in nonhuman primates. 02 November 2022, PREPRINT (Version 1) available at Research Square https://doi.org/10.21203/rs.3.rs-2013762/v1 [Article]
user_report Funaki, T., CADM1 promotes malignant features of small-cell lung cancer by recruiting 4.1R to the plasma membrane. Biochem. Biophys. Res. Commun. 534: 172-178 (2021). PMID 33298314. [PubMed] [Article] [RRC of NBRP]
user_report Totani, H., Autocrine HGF/c-Met signaling pathway confers aggressiveness in lymph node adult T-cell leukemia/lymphoma. Oncogene 39 (35): 5782-5794 (2020). PMID 32747750. [PubMed] [Article] [RRC of NBRP]
user_report Echizen, K., NF-kappaB-induced NOX1 activation promotes gastric tumorigenesis through the expansion of SOX2-positive epithelial cells. Oncogene 38 (22): 4250-4263 (2019). PMID 30700829. [PubMed] [Article] [RRC of NBRP]
user_report Ito, T., Mathematical analysis of gefitinib resistance of lung adenocarcinoma caused by MET amplification. Biochem. Biophys. Res. Commun. 511 (3): 544-550 (2019). PMID 30824185. [PubMed] [Article] [RRC of NBRP]
user_report Kawasaki, Y., he novel lncRNA CALIC upregulates AXL to promote colon cancer metastasis. EMBO Rep. 20 (8): e47052 (2019). PMID 31353791. [PubMed] [Article] [RRC of NBRP]
user_report Funato, K., SIRT2-mediated inactivation of p73 is required for glioblastoma tumorigenicity. EMBO Rep. 19 (11). pii: e45587 (2018). PMID 30213795. [PubMed] [Article] [RRC of NBRP]
user_report Taniue, K., Long noncoding RNA UPAT promotes colon tumorigenesis by inhibiting degradation of UHRF1. Proc. Natl. Acad. Sci. U.S.A. 113 (5): 1273-1278 (2016). PMID 26768845. [PubMed] [Article] [RRC of NBRP]
user_report Taniue, K., ASBEL-TCF3 complex is required for the tumorigenicity of colorectal cancer cells. Proc. Natl. Acad. Sci. U.S.A. 113 (45): 12739–12744 (2016). PMID 27791078. [PubMed] [Article] [RRC of NBRP]
user_report Hiraoka, K., SOX9-mediated upregulation of LGR5 is important for glioblastoma tumorigenicity. Biochem. Biophys. Res. Commun. 460 (2): 216-221 (2015). PMID 25770425. [PubMed] [Article] [RRC of NBRP]
user_report Hashizume, O., Epigenetic regulation of the nuclear-coded GCAT and SHMT2 genes confers human age-associated mitochondrial respiration defects. Sci. Rep. 5: 10434 (2015). PMID 26000717. [PubMed] [Article] [RRC of NBRP]
user_report Koyama-Nasu, R., The pleiotrophin-ALK axis is required for tumorigenicity of glioblastoma stem cells. Oncogene, 33 (17): 2236-2244 (2014). PMID 23686309. [PubMed] [Article] [RRC of NBRP]
user_report Ito, R., TET3-OGT interaction increases the stability and the presence of OGT in chromatin. Genes Cells, 19 (1): 52-65 (2014). PMID 24304661. [PubMed] [Article] [RRC of NBRP]
user_report Koyama-Nasu, R., The critical role of cyclin D2 in cell cycle progression and tumorigenicity of glioblastoma stem cells. Oncogene, 32 (33): 3840-3845 (2013). PMID 22964630. [PubMed] [Article] [RRC of NBRP]
user_report Koyama-Nasu, R, The cancer stem cell marker CD133 interacts with plakoglobin and controls desmoglein-2 protein levels. PLoS One, 8 (1): e53710 (2013). PMID 23326490. [PubMed] [Article] [RRC of NBRP]
user_report Yanagida, S., ASBEL, an ANA/BTG3 antisense transcript required for tumorigenicity of ovarian carcinoma. Sci. Rep. 2013 Feb 19;3:1305 PMID 23419616. [PubMed] [Article] [RRC of NBRP]
user_report Fukushima K., FIP1L1-PDGFRalpha imposes eosinophil lineage commitment on hematopoietic stem/progenitor cells. J. Biol. Chem., 284, 7719-7732 (2009). PMID 19147501. [PubMed] [Article] [RRC of NBRP]