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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

CSII-EF-RfA-IRES2-Venus

Backbone vector plasmid of lentivirus construct driven by EF-1 alpha promoter by Gateway(R) cloning with Venus fluorescent protein marker.

Catalog number RDB04389
Resource name CSII-EF-RfA-IRES2-Venus
Clone info. Backbone vector plasmid of lentivirus construct driven by EF-1 alpha promoter by Gateway(R) cloning with Venus fluorescent protein marker.
Comment Commonly requested with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Vector backbone CSII-EF-RfA-IRES2-Venus (Plasmid)
Selectable markers Please note that Zeo resistance marker is not included in the lentivirus produced from this vector. E. coli: Amp^r, Cm^r, ccdB.
Growth remarks Use DB3.1, ccdB Survival or equivalent to amplify this clone. 30 degC is fine.
Gene/insert name Aequorea victoria GFP cDNA
Depositor|Developer Miyoshi, Hiroyuki |
Other clones in our bank

External Database
Aequorea victoria GFP

            Reference sequence
              
             
            Sequence RDB04389z.seq
            Remarks, protocol and/or map (pdf) RDB04389.pdf
            Lentiviral plasmid list. [link]

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer.
            Additional terms and conditions for distribution RECIPIENT is requested to obtain written consent of Dr. Atsushi Miyawaki of the RIKEN CBS regarding Venus fluorescent protein.
            Ordering Please visit Ordering instruction.[link] 
            Order form 
            [Credit Card Payment]  [Bank Transfer Payment]
            Material Transfer Agreement (MTA, for use for not-for-profit academic purpose)[Word]
            Approval form [Word] by Dr. Atsushi Miyawaki
            Remarks Remember that you will be working with samples containing infectious virus.
            Use Survival2 (Invitrogen) or equivalent to amplify this clone.
            Please obtain written approval of Dr. Atsushi Miyawaki.
            提供案内 (日本国内) [open/close]

            提供条件 本件研究材料は、非営利機関の非営利学術研究に限って提供する。本件研究材料を利用した研究結果等を発表する際は、本件研究材料が故三好浩之博士により開発されたことを明示する。
            付加的提供条件 利用者は、Venus蛍光タンパク質について理化学研究所CBS 宮脇敦史博士から、書面による提供承諾を得ること。
            提供依頼 手続きの詳細は、「レンチウイルスベクターの提供申し込み」をご覧ください。
            提供依頼書 [Word]
            提供同意書 (非営利機関による非営利学術研究用)[Word]
            遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。
            宮脇敦史博士による提供承諾書 [Word]
            備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。
            このクローンの増殖にはSurvival2 (Invitrogen)あるいは同等の宿主を使用してください。
            宮脇敦史博士の書面による提供承諾が必要です。

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB04389 CSII-EF-RfA-IRES2-Venus DNA solution

            Ordering instruction of plasmids [in Japanese] [in English]
            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Original reference

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Lentivirus Vector (English text)
            Featured content Lentivirus Vector (Japanese text)

            QC test results

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB08955_A2Kfp1-3.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: pAxEF_F1
            Region: RfA(attR1,CmR)
            Sequence file: RDB08955_A2Kfh.seq check
            >04389_08955_A2Kf_pAxEFF1_C05_08.ab1
                1 NNNNNNNNNN NCNTTNNNGG NGTCGNNNNN NGCTACCGGT CTCGAGAATT CTGCAGCGGC
               61 CATCACAAGT TTGTACAAAA AAGCTGAACG AGAAACGTAA AATGATATAA ATATCAATAT
              121 ATTAAATTAG ATTTTGCATA AAAAACAGAC TACATAATAC TGTAAAACAC AACATATCCA
              181 GTCACTATGG CGGCCGCATT AGGCACCCCA GGCTTTACAC TTTATGCTTC CGGCTCGTAT
              241 AATGTGTGGA TTTTGAGTTA GGATCCGTCG AGATTTTCAG GAGCTAAGGA AGCTAAAATG
              301 GAGAAAAAAA TCACTGGATA TACCACCGTT GATATATCCC AATGGCATCG TAAAGAACAT
              361 TTTGAGGCAT TTCAGTCAGT TGCTCAATGT ACCTATAACC AGACCGTTCA GCTGGATATT
              421 ACGGCCTTTT TAAAGACCGT AAAGAAAAAT AAGCACAAGT TTTATCCGGC CTTTATTCAC
              481 ATTCTTGCCC GCCTGATGAA TGCTCATCCG GAATTCCGTA TGGCAATGAA AGACGGTGAG
              541 CTGGTGATAT GGGATAGTGT TCACCCTTGT TACACCGTTT TCCATGAGCA AACTGAAACG
              601 TTTTCATCGC TCTGGAGTGA ATACCACGAC GATTTCCGGC AGTTTCTACA CATATATTCG
              661 CAAGATGTGG CGTGTTACGG TGAAAACCTG GCCTATTTCC CTAAAGGGTT TATTGAGAAT
              721 ATGTTTTTCG TCTCAGCCAA TCCCTGGGTG AGTTTCACCA GTTTTGATTT AAACGTGGCC
              781 AATATGGACA ACTTCTTCGC CCCCGTTTTC ACCATGGGNA AATANTATAC GCAAGGCGAC
              841 AAGGTGCTGA NGCCGCTGGC GATTCANGTT CATCATGNCN TTNGTGATGG CTTNNNNGTC
              901 NGCAGAANGN TNNATGAATT ACAACAGNAC TGCGATGANT NGNNNNNNGG GCGNNANCNC
              961 NNNNNNNCNG NTTANNAAAA GCCANANAAC AGTANGNNTA NTNNCNCGCT GANTTTNNNN
            1021 GNNNNNNNNA TANANNNNGN NNNNNNNNN
            //
            Primer: IRES-R
            Region: RfA(attR2,ccdB)
            Sequence file: RDB08955_A2Kfi.seq check
            >04389_08955_A2Kf_IRES-R_G08_20.ab1
                1 NNNNNNNNNN NNNNNNGNNN NNNNGTTAGG GGGGGGGGNN GGNNANGGGC GGNNCCGCGG
               61 CCATCACCAC TTTGTACANN AAAGCTGAAC GAGAAACGTA AAATGATATA AATATCAATA
              121 TATTAAATTA GATTTTGCAT AAAAAACAGA CTACATAATA CTGTAAAACA CAACATATCC
              181 AGTCACTATG GTCGACCTGC AGACTGGCTG TGTATAAGGG AGCCTGACAT TTATATTCCC
              241 CAGAACATCA GGTTAATGGC GTTTTTGATG TCATTTTCGC GGTGGCTGAG ATCAGCCACT
              301 TCTTCCCCGA TAACGGAGAC CGGCACACTG GCCATATCGG TGGTCATCAT GCGCCAGCTT
              361 TCATCCCCGA TATGCACCAC CGGGTAAAGT TCACGGGAGA CTTTATCTGA CAGCAGACGT
              421 GCACTGGCCA GGGGGATCAC CATCCGTCGC CCGGGCGTGT CAATAATATC ACTCTGTACA
              481 TCCACAAACA GACGATAACG GCTCTCTCTT TTATAGGTGT AAACCTTAAA CTGCATTTCA
              541 CCAGCCCCTG TTCTCGTCAG CAAAAGAGCC GTTCATTTCA ATAAACCGGG CGACCTCAGC
              601 CATCCCTTCC TGATTTTCCG CTTTCCAGCG TTCGGCACGC AGACGACGGG CTTCATTCTG
              661 CATGGTTGTG CTTACCAGAC CGGANATATT GACATCATAT ATGCCTTGAG CAACTGATAG
              721 CTGTCGCTGT CAACTGTCAC TGTAATACGC TGCTTCATAG CATACCTCTT TTTGACATAC
              781 TTCGGGTATA CATATCAGTA TATATTCTTA TACCGCAAAA ATCAGCGCGC AAATACGCAT
              841 ACTGTTATCT GGCTTTTAGT AAGCCGGATN CACGCGTTTA CGCCCNNCCC NGNNNCTCAT
              901 CNNANTACTG TTGNNANTCA NTAANCATTC TGNNNANNTG GAAGCCNTCN CNNNNGGNNN
            961 GATNNNNNTG NANNGNNNGC NGNNTCANCN CCNNNNCNNN NNNNNNN
            //
            Primer: IRES-F
            Region: IRES2,Venus 5'
            Sequence file: RDB08955_A2Kfj.seq check
            >04389_08955_A2Kf_IRES-F_H08_23.ab1
                1 NNNNNNNNNN NNNNNNTNNG TCGANGTNNA AAAAACGTCT AGGCCCCCNN AACCNCGGGG
               61 NCGTGGTTTN NNNNNNNNNA ACACGATGAT AATATGGCCA CAACCATGGT GAGCAAGGGC
              121 GAGGAGCTGT TCACCGGGGT GGTGCCCATC CTGGTCGAGC TGGACGGCGA CGTAAACGGC
              181 CACAAGTTCA GCGTGTCCGG CGAGGGCGAG GGCGATGCCA CCTACGGCAA GCTGACCCTG
              241 AAGCTGATCT GCACCACCGG CAAGCTGCCC GTGCCCTGGC CCACCCTCGT GACCACCCTG
              301 GGCTACGGCC TGCAGTGCTT CGCCCGCTAC CCCGACCACA TGAAGCAGCA CGACTTCTTC
              361 AAGTCCGCCA TGCCCGAAGG CTACGTCCAG GAGCGCACCA TCTTCTTCAA GGACGACGGC
              421 AACTACAAGA CCCGCGCCGA GGTGAAGTTC GAGGGCGACA CCCTGGTGAA CCGCATCGAG
              481 CTGAAGGGCA TCGACTTCAA GGAGGACGGC AACATCCTGG GGCACAAGCT GGAGTACAAC
              541 TACAACAGCC ACAACGTCTA TATCACCGCC GACAAGCAGA AGAACGGCAT CAAGGCCAAC
              601 TTCAAGATCC GCCACAACAT CGAGGACGGC GGCGTGCAGC TCGCCGACCA CTACCAGCAG
              661 AACACCCCCA TCGGCGACGG CCCCGTGCTG CTGCCCGACA ACCACTACCT GAGCTACCAG
              721 TCCGCCCTGA GCAAAGACCC CAACGAGAAG CGCGATCACA TGGTCCTGCT GGAGTTCGTG
              781 ANCGCCGCCG GGATCACTCT CGGCATGGAC GANCTGTACA AGTAAGAATT CNAGGCCTCT
              841 CGANCCTCTA NAGTTNACAT CGAGGGATCA AGCTTATCGA TAATCNNNCT CTGGNNTANN
              901 AAATTTGTGA AANATTGACT GGTATTCTTN NCTATGTTGC TCCTTTNACN NTATGTGNAT
              961 ACNCTGNTTT NANGNNNTTG TATCATGCTN NNGNTNNCNN NATGGNTTNC NNTTTNNNNC
            1021 NNNCNNNNNN NNANNNNNNN NNN
            //
            Primer: PRE_rev
            Region: PRE,Venus 3'
            Sequence file: RDB08955_A2Kfk.seq check
            >04389_08955_B2HfF_PRE_rev_A07_01_ABI08.ab1
                1 NNNNNNNNNN NNNNNCNNNN NNGAGCANCA TAGTTAAGAA TACCAGTCAA TCTTTCACAA
               61 ATTTTGTAAT CCAGAGGTTG ATTATCGATA AGCTTGATCC CTCGATGTTA ACTCTAGAGG
              121 CTCGAGAGGC CTTGAATTCT TACTTGTACA GCTCGTCCAT GCCGAGAGTG ATCCCGGCGG
              181 CGGTCACGAA CTCCAGCAGG ACCATGTGAT CGCGCTTCTC GTTGGGGTCT TTGCTCAGGG
              241 CGGACTGGTA GCTCAGGTAG TGGTTGTCGG GCAGCAGCAC GGGGCCGTCG CCGATGGGGG
              301 TGTTCTGCTG GTAGTGGTCG GCGAGCTGCA CGCCGCCGTC CTCGATGTTG TGGCGGATCT
              361 TGAAGTTGGC CTTGATGCCG TTCTTCTGCT TGTCGGCGGT GATATAGACG TTGTGGCTGT
              421 TGTAGTTGTA CTCCAGCTTG TGCCCCAGGA TGTTGCCGTC CTCCTTGAAG TCGATGCCCT
              481 TCAGCTCGAT GCGGTTCACC AGGGTGTCGC CCTCGAACTT CACCTCGGCG CGGGTCTTGT
              541 AGTTGCCGTC GTCCTTGAAG AAGATGGTGC GCTCCTGGAC GTAGCCTTCG GGCATGGCGG
              601 ACTTGAAGAA GTCGTGCTGC TTCATGTGGT CGGGGTAGCG GGCGAAGCAC TGCAGGCCGT
              661 AGCCCAGGGT GGTCACGAGG GTGGGCCAGG GCACGGGCAG CTTGCCGGTG GTGCAGATCA
              721 GCTTCAGGGT CAGCTTGCCG TANGTGGCAT CGCCCTCGCC CTCGCCGGAC ACGCTGAACT
              781 TGTGGCCGTT TACGTCGCCG TCCAGCTCGA CCAGGATGGG CACCACCCCG GTGAACAGCT
              841 CCTCGCCCTT GCTCACCATG GTTGTGGNCA TATTATCATC GTGTTTTTCA AAGGAAAANC
              901 ACGTCCCCGT GGTNCNGGGG NNCTANACGT TTTTTTAACC TCNANTAANC NCATGTAAAG
              961 CATGTGTANC GAGGCCCCAN NTNANATNCC ATACNNTGGG GTANCNNCTG GGCNTNNNTC
            1021 ANCCCCNNNN NGNATANNCN NGNNNNNNNN ATTNGNNNCN TTNNNNNNNN NN
            //
            Primer: CMV_Forward
            Region: CMV pro,5'LTR,HIV-1 psi
            Sequence file: RDB08955_A2Kfl.seq check
            >04389_08955_A2Kf_CMV-Foward_E08_14.ab1
                1 NNNNNNNNNN NNNNNNCNTT GNNGCNNNTG GGCGGTAGGC GTGTACGGTG GGAGGTCTAT
               61 ATAAGCAGCG CGTTTTGCCT GTACTGGGTC TCTCTGGTTA GACCAGATCT GAGCCTGGGA
              121 GCTCTCTGGC TAACTAGGGA ACCCACTGCT TAAGCCTCAA TAAAGCTTGC CTTGAGTGCT
              181 TCAAGTAGTG TGTGCCCGTC TGTTGTGTGA CTCTGGTAAC TAGAGATCCC TCAGACCCTT
              241 TTAGTCAGTG TGGAAAATCT CTAGCAGTGG CGCCCGAACA GGGACTTGAA AGCGAAAGGG
              301 AAACCAGAGG AGCTCTCTCG ACGCAGGACT CGGCTTGCTG AAGCGCGCAC GGCAAGAGGC
              361 GAGGGGCGGC GACTGGTGAG TACGCCAAAA ATTTTGACTA GCGGAGGCTA GAAGGAGAGA
              421 GATGGGTGCG AGAGCGTCAG TATTAAGCGG GGGAGAATTA GATCGCGATG GGAAAAAATT
              481 CGGTTAAGGC CAGGGGGAAA GAAAAAATAT AAATTAAAAC ATATAGTATG GGCAAGCAGG
              541 GAGCTAGAAC GATTCGCAGT TAATCCTGGC CTGTTAGAAA CATCAGAAGG CTGTAGACAA
              601 ATACTGGGAC AGCTACAACC ATCCCTTCAG ACAGGATCAG AAGAACTTAG ATCATTATAT
              661 AATACAGTAG CAACCCTCTA TTGTGTGCAT CAAAGGATAG AGATAAAAGA CACCAAGGAA
              721 GCTTTAGACA AGATAGAGGA AGAGCAAAAC AAAAGTAAGA CCACCGCACA GCAAGGGCCG
              781 CTGATCTTCA GACCTGGAGG AGGANATATG AGGGANAATT GGAGAAGTGA ATTATATAAA
              841 TATAAAGTAG TAAAAATTGA ANCATTANGA GTANCACCCA CNNANNNNNN GAGAANANNN
              901 NTGCANANAG AAAAAAGANC AGNGGGAATA GGANCTTTGT TCCNTGGGTT CTTGGGANCN
            961 GCNNNAAGCN CTATGGGNNN NNCNNNNNNG ACGCNNNNNG NTNNNNNNNN NN
            //
            Primer: cPPT/CTS-E_R
            Region: cPPT_CTS,RRE
            Sequence file: RDB08955_A2Kfm.seq check
            >04389_08955_B2HfF_cPPT_CTS-E_R_B05_02_ABI08.ab1
                1 NNNNNNNNNN NNNGNTTTTN NNNTTCTTTA GTTTGTATGT CTGTTGCTAT TATGTCTACT
               61 ATTCTTTCCC CTGCACTGTA CCCCCCAATC CCCCCTTTTC TTTTAAAATT GTGGATGAAT
              121 ACTGCCATTT GTAGATCCGT TCACTAATCG AATGGATCTG TCTCTGTCTC TCTCTCCACC
              181 TTCTTCTTCT ATTCCTTCGG GCCTGTCGGG TCCCCTCGGG GTTGGGAGGT GGGTCTGAAA
              241 CGATAATGGT GAATATCCCT GCCTAACTCT ATTCACTATA GAAAGTACAG CAAAAACTAT
              301 TCTTAAACCT ACCAAGCCTC CTACTATCAT TATGAATAAT TTTATATACC ACAGCCAATT
              361 TGTTATGTTA AACCAATTCC ACAAACTTGC CCATTTATCT AATTCCAATA ATTCTTGTTC
              421 ATTCTTTTCT TGCTGGTTTT GCGATTCTTC AATTAAGGAG TGTATTAAGC TTGTGTAATT
              481 GTTAATTTCT CTGTCCCACT CCATCCAGGT CGTGTGATTC CAAATCTGTT CCAGAGATTT
              541 ATTACTCCAA CTAGCATTCC AAGGCACAGC AGTGGTGCAA ATGAGTTTTC CAGAGCAACC
              601 CCAAATCCCC AGGAGCTGTT GATCCTTTAG GTATCTTTCC ACAGCCAGGA TTCTTGCCTG
              661 GAGCTGCTTG ATGCCCCAGA CTGTGAGTTG CAACAGATGC TGTTGCGCCT CAATAGCCCT
              721 CAGCAAATTG TTCTGCTGCT GCACTATACC AGACAATAAT TGTCTGGCCT GTACCGTCAG
              781 CGTCATTGAC GCTGCGCCCA TAGTGCTTCC TGCTGCTCCC NAGAACCCAA NGAACAAAGC
              841 TCCTATTCCC ACTGCTCTTT TTTCTCTCTG CACCACTCTT CTCTTTGCCT TGGNGGGTGC
              901 TANTCCNNAT GNNNCAATTT TTACTACTTT ANATTTATAT AATTCACTTC TCCNATTGNC
              961 CCTNATATCT CCTNCNCNAG NTCTGAANAT NANCGGCCCN NGCTGTGNNN NGNNNNNNNT
            1021 TTNGTTTNNN NNNNNCNNNA TNNNNNNNNA NNNNNNNNNN
            //
            Primer: bGH_rev3
            Region: bGH pA,3'LTR
            Sequence file: RDB08955_A2Kfn.seq check
            >04389_08955_B2HfF_bGH_rev3_C05_03_ABI08.ab1
                1 NNNNNNNNNN NNNNNNNNGG NGGNNGGGCN AACNANAGAT GGCTGGCAAC TAGAAGGCAC
               61 AGTCGAGGCT GATCAGCGGG TTTAAACGGG CCCTGCTAGA GATTTTCCAC ACTGACTAAA
              121 AGGGTCTGAG GGATCTCTAG TTACCAGAGT CACACAACAG ACGGGCACAC ACTACTTGAA
              181 GCACTCAAGG CAAGCTTTAT TGAGGCTTAA GCAGTGGGTT CCCTAGTTAG CCAGAGAGCT
              241 CCCAGGCTCA GATCTGGTCT AACCAGAGAG ACCCAGTACA GTCCGGATGC AGCTCTCGGG
              301 CCATGTGATG AAATGCTAGG CGGCTGTCAA ACCTCCACTC TAATACTTCT CTCTCCGGGT
              361 CATCCATCCC ATGCAGGCTC ACAGGGTGTA ACAAGCGGGT GTTCTCTCCT TCATTGGCTT
              421 CTTCTACCTT CTCTTGCTCA ACTGGTACTA GCTTGTAGCA CCATCCAAAG GTCAGTGGAT
              481 ATCTGATCCC TGGCCCTGGT GTGTAGTTCT GCCAATCAGG GAAGTAGCCT TGTGTGTGGT
              541 AGATCCACAG ATCAAGGATA TCTTGTCTTC GTTGGGAGTG AATTAGCCCT TCCAGTCCCC
              601 CCTTTTCTTT TAAAAAGTGG CTAAGATCTA CAGCTGCCTT GTAAGTCATT GGTCTTAAAG
              661 GTACCTGAGG TGTGACTGGA AAACCCACCT CCTCCTCCTC TTGTGCTTCT AGCCAGGCAC
              721 AATCAGCATT GGTAGCTGCT GTATTGCTAC TTGTGATTGC TCCATGTTTT TCTAGGTCTC
              781 GACGGTATCG ATGCGGGGAN GCGGCCNNAA GGGAGATCCG ANTCGTCTGA NGGCGAANGC
              841 GAAGACNCGG AAGANGCCGC ANANCCNGCA GCANGNCGCG GGAANGANNG TCCGCTGGAT
              901 TGANGGNCGA NNGGANGTAN CANANNGNNG TNCCGCGCAN ANTCCAGNTG GNAACNCNNN
              961 NANCANNNNT GGAAAGGNNN TCNNCTTCCC CNNNNNCNNN ANNNANNTGN CANTGCCCNN
            1021 CNNNCNNANC CCCNNNNNNN CANCNGGNNN NNNNNNN
            //
            Primer: SV40pro_F
            Region: SV40 pro_ori,Zeo
            Sequence file: RDB08955_A2Kfo.seq check
            >04389_08955_A2Kf_SV40pro_F_F08_17.ab1
                1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNGT GNNGANNNTT TTTTGGAGGC CTAGGCTTTT
               61 GCAAAAAGCT CCCGGGAGCT TGNNTANCCN TTTTCGGATC TGATCAGCAC GTGTTGACAA
              121 TTAATCATCG GCATAGTATA TCGGCATAGT ATAATACGAC AAGGTGAGGA ACTAAACCAT
              181 GGCCAAGTTG ACCAGTGCCG TTCCGGTGCT CACCGCGCGC GACGTCGCCG GAGCGGTCGA
              241 GTTCTGGACC GACCGGCTCG GGTTCTCCCG GGACTTCGTG GAGGACGACT TCGCCGGTGT
              301 GGTCCGGGAC GACGTGACCC TGTTCATCAG CGCGGTCCAG GACCAGGTGG TGCCGGACAA
              361 CACCCTGGCC TGGGTGTGGG TGCGCGGCCT GGACGAGCTG TACGCCGAGT GGTCGGAGGT
              421 CGTGTCCACG AACTTCCGGG ACGCCTCCGG GCCGGCCATG ACCGAGATCG GCGAGCAGCC
              481 GTGGGGGCGG GAGTTCGCCC TGCGCGACCC GGCCGGCAAC TGCGTGCACT TCGTGGCCGA
              541 GGAGCAGGAC TGACACGTGC TACGAGATTT CGATTCCACC GCCGCCTTCT ATGAAAGGTT
              601 GGGCTTCGGA ATCGTTTTCC GGGACGCCGG CTGGATGATC CTCCAGCGCG GGGATCTCAT
              661 GCTGGAGTTC TTCGCCCACC CCAACTTGTT TATTGCAGCT TATAATGGTT ACAAATAAAG
              721 CAATAGCATC ACAAATTTCA CAAATAAAGC ATTTTTTTCA CTGCATTCTA NTTGTGGTTT
              781 GTCNNAACTC ATCAATGTAT CTTATCATGT CTGTATACCG TCGACCTCTA GCTAGAGCTT
              841 GNCGTAATCA TGGTCATAGC TGTTTCCTGT GTGAAATTGT TATCCGCTCA CAATTCCNCA
              901 CAACATACGA NCCGGAANCA NNAAGNGTAA AGCCTGGGGG NGCCNAATGA NNGANCTNAC
              961 TCNCATTAAT TGCGTTGCGC NCACTGNCCN CTTTNNNNNN NNNGNAANNN NNNNNNGNCN
            1021 NCNNNNNNNA NGNNNNNGNC NNANCNNNNN NNNNN
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            user_report Watanabe, H., Flexible and Accurate Substrate Processing with Distinct Presenilin/gamma-Secretases in Human Cortical Neurons eNeuro 8 (2): ENEURO.0500-20.2021 (2021). PMID 33608391.
            user_report Yoshitomi, H., Human Sox4 facilitates the development of CXCL13-producing helper T cells in inflammatory environments. Nat. Commun. 9 (1): 3762 (2018). PMID 30232328.
            user_report Naka, H., Requirement for COUP-TFI and II in the temporal specification of neural stem cells in CNS development. Nat. Neurosci. 11 (9): 1014-1023 (2008). PMID 19160499.

            2022.10.05

            GNP_filter3_RDBDEP_html_220812.pl