Please review the QC test results indicated by icon below as well as clone information before placing your order.

CSII-EF-RfA-IRES2-Venus

Backbone vector plasmid of lentivirus construct driven by EF-1 alpha promoter by Gateway(R) cloning with Venus fluorescent protein marker.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer. Additional terms and conditions: RECIPIENT is requested to obtain written consent of Dr. Atsushi Miyawaki of the RIKEN CBS regarding Venus fluorescent protein.
Ordering	Please visit Ordering instruction.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA, for use for not-for-profit academic purpose)[Word] Approval form [Word] by Dr. Atsushi Miyawaki
Remarks	Remember that you will be working with samples containing infectious virus. Use Survival2 (Invitrogen) or equivalent to amplify this clone. Please obtain written approval of Dr. Atsushi Miyawaki.

提供案内 (日本国内) [open/close]

提供条件	本件研究材料は、非営利機関の非営利学術研究に限って提供する。本件研究材料を利用した研究結果等を発表する際は、本件研究材料が故三好浩之博士により開発されたことを明示する。 付加的提供条件： 利用者は、Venus蛍光タンパク質について理化学研究所CBS 宮脇敦史博士から、書面による提供承諾を得ること。
提供依頼	手続きの詳細は、「レンチウイルスベクターの提供申し込み」をご覧ください。 提供依頼書 [Word] 提供同意書 (非営利機関による非営利学術研究用)[Word] 遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。 宮脇敦史博士による提供承諾書 [Word]
備考	このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。 このクローンの増殖にはSurvival2 (Invitrogen)あるいは同等の宿主を使用してください。 宮脇敦史博士の書面による提供承諾が必要です。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

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Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB08955_A2Kfp1-2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxEF_F1 Sequence file: RDB08955_A2Kfa.seq
>04389_08955_A2Kf_pAxEFF1_C05_08.ab1     1 ATTTTTTTCT TCATTTCAGG TGTCGTGACA CGCTACCGGT CTCGAGAATT CTGCAGCGGC    61 CATCACAAGT TTGTACAAAA AAGCTGAACG AGAAACGTAA AATGATATAA ATATCAATAT   121 ATTAAATTAG ATTTTGCATA AAAAACAGAC TACATAATAC TGTAAAACAC AACATATCCA   181 GTCACTATGG CGGCCGCATT AGGCACCCCA GGCTTTACAC TTTATGCTTC CGGCTCGTAT   241 AATGTGTGGA TTTTGAGTTA GGATCCGTCG AGATTTTCAG GAGCTAAGGA AGCTAAAATG   301 GAGAAAAAAA TCACTGGATA TACCACCGTT GATATATCCC AATGGCATCG TAAAGAACAT   361 TTTGAGGCAT TTCAGTCAGT TGCTCAATGT ACCTATAACC AGACCGTTCA GCTGGATATT   421 ACGGCCTTTT TAAAGACCGT AAAGAAAAAT AAGCACAAGT TTTATCCGGC CTTTATTCAC   481 ATTCTTGCCC GCCTGATGAA TGCTCATCCG GAATTCCGTA TGGCAATGAA AGACGGTGAG   541 CTGGTGATAT GGGATAGTGT TCACCCTTGT TACACCGTTT TCCATGAGCA AACTGAAACG   601 TTTTCATCGC TCTGGAGTGA ATACCACGAC GATTTCCGGC AGTTTCTACA CATATATTCG   661 CAAGATGTGG CGTGTTACGG TGAAAACCTG GCCTATTTCC CTAAAGGGTT TATTGAGAAT   721 ATGTTTTTCG TCTCAGCCAA TCCCTGGGTG AGTTTCACCA GTTTTGATTT AAACGTGGCC   781 AATATGGACA ACTTCTTCGC CCCCGTTTTC ACCATGGGCA AATATTATAC GCAAGGCGAC   841 AAGGTGCTGA TGCCGCTGGC GATTCAGGTT CATCATGCCG TTTGTGATGG CTTCCATGTC   901 GGCAGAATGC TTAATGAATT ACAACAGTAC TGCGATGAGT GGCAGGCGGG GCGTAAACGC   961 GTGGATCCGG CTTACTAAAA GCCAGATAAC AGTATGCGTA TTTGCGCGCT GATTTTTGCG  1021 GGTATAAGAA TATATACTGA TATGTAATAC CCGAAGTATG TCAAAAAAGA GGTATGCTAT  1081 GAAAGCAGCG TATTACAGTG ACAGTTGAAC AGCGAACAGG CTATCAGTGG CTCAGCCATA 1141 ATATGATGGT CAATATCTCC GGGTCTTGGG TAAGCCACAA CCCAATGGCC CA //
Primer: IRES-R Sequence file: RDB08955_A2Kfb.seq
>04389_08955_A2Kf_IRES-R_D04_10.ab1     1 GCAAATGCCG GGCTTCGGGC AGTAACGTTA GGGGGGGGGG AGGGAGAGGG GCGGATCCGC    61 GGCCATCACC ACTTTGTACA AGAAAGCTGA ACGAGAAACG TAAAATGATA TAAATATCAA   121 TATATTAAAT TAGATTTTGC ATAAAAAACA GACTACATAA TACTGTAAAA CACAACATAT   181 CCAGTCACTA TGGTCGACCT GCAGACTGGC TGTGTATAAG GGAGCCTGAC ATTTATATTC   241 CCCAGAACAT CAGGTTAATG GCGTTTTTGA TGTCATTTTC GCGGTGGCTG AGATCAGCCA   301 CTTCTTCCCC GATAACGGAG ACCGGCACAC TGGCCATATC GGTGGTCATC ATGCGCCAGC   361 TTTCATCCCC GATATGCACC ACCGGGTAAA GTTCACGGGA GACTTTATCT GACAGCAGAC   421 GTGCACTGGC CAGGGGGATC ACCATCCGTC GCCCGGGCGT GTCAATAATA TCACTCTGTA   481 CATCCACAAA CAGACGATAA CGGCTCTCTC TTTTATAGGT GTAAACCTTA AACTGCATTT   541 CACCAGCCCC TGTTCTCGTC AGCAAAAGAG CCGTTCATTT CAATAAACCG GGCGACCTCA   601 GCCATCCCTT CCTGATTTTC CGCTTTCCAG CGTTCGGCAC GCAGACGACG GGCTTCATTC   661 TGCATGGTTG TGCTTACCAG ACCGGAGATA TTGACATCAT ATATGCCTTG AGCAACTGAT   721 AGCTGTCGCT GTCAACTGTC ACTGTAATAC GCTGCTTCAT AGCATACCTC TTTTTGACAT   781 ACTTCGGGTA TACATATCAG TATATATTCT TATACCGCAA AAATCAGCGC GCAAATACGC   841 ATACTGTTAT CTGGCTTTTA GTAAGCCGGA TCCACGCGTT TACGCCCCGC CCTGCCACTC   901 ATCGCAGTAC TGTTGTAATT CATTAAGCAT TCTGCCGACA TGGAAGCCAT CACAAACGGC   961 ATGATGAACC TGAATCGCCA GCGGCATCAG CACCTTGTCG CCCTTGCGTA TAATATTTGC  1021 CCATGGGTGA AAACGGGGGG GCGAAAGAAG TTGTTCCATA TTGGCCACGT TTTAAATCAA  1081 AACTGTGAAC TCACCCAGGA TTGGCTGGAG ACGAAACCAT ATTCTCAATA AACCGTTTAG 1141 GGAAAATAGG CCCAGGTTTT T //
Primer: IRES-F Sequence file: RDB08955_A2Kfc.seq
>04389_08955_A2Kf_IRES-F_E04_13.ab1     1 CGAGCGTTTA ATGTGTTTAG TCGAGGTTAA AAAAACGTCT AGGCCCCCCG AACCACGGGG    61 ACGTGGTTTT CCTTTGAAAA ACACGATGAT AATATGGCCA CAACCATGGT GAGCAAGGGC   121 GAGGAGCTGT TCACCGGGGT GGTGCCCATC CTGGTCGAGC TGGACGGCGA CGTAAACGGC   181 CACAAGTTCA GCGTGTCCGG CGAGGGCGAG GGCGATGCCA CCTACGGCAA GCTGACCCTG   241 AAGCTGATCT GCACCACCGG CAAGCTGCCC GTGCCCTGGC CCACCCTCGT GACCACCCTG   301 GGCTACGGCC TGCAGTGCTT CGCCCGCTAC CCCGACCACA TGAAGCAGCA CGACTTCTTC   361 AAGTCCGCCA TGCCCGAAGG CTACGTCCAG GAGCGCACCA TCTTCTTCAA GGACGACGGC   421 AACTACAAGA CCCGCGCCGA GGTGAAGTTC GAGGGCGACA CCCTGGTGAA CCGCATCGAG   481 CTGAAGGGCA TCGACTTCAA GGAGGACGGC AACATCCTGG GGCACAAGCT GGAGTACAAC   541 TACAACAGCC ACAACGTCTA TATCACCGCC GACAAGCAGA AGAACGGCAT CAAGGCCAAC   601 TTCAAGATCC GCCACAACAT CGAGGACGGC GGCGTGCAGC TCGCCGACCA CTACCAGCAG   661 AACACCCCCA TCGGCGACGG CCCCGTGCTG CTGCCCGACA ACCACTACCT GAGCTACCAG   721 TCCGCCCTGA GCAAAGACCC CAACGAGAAG CGCGATCACA TGGTCCTGCT GGAGTTCGTG   781 ACCGCCGCCG GGATCACTCT CGGCATGGAC GAGCTGTACA AGTAAGAATT CAAGGCCTCT   841 CGAGCCTCTA GAGTTAACAT CGAGGGATCA AGCTTATCGA TAATCAACCT CTGGATTACA   901 AAATTTGTGA AAGATTGACT GGTATTCTTA ACTATGTTGC TCCTTTTACG CTATGTGGAT   961 ACGCTGCTTT AATGCCTTTG TATCATGCTA TTGCTTCCCG TATGGCTTTC ATTTTCTCCT  1021 CCCTTGTATA AATCCTGGAT GCTGTCTCTT TATGAGAGTT GTGGCCCGAT GTCAGCAACG  1081 TGACGTGATG TGCACTGTGT TTGCCTGGAC GGCACCCCAA CTGATGGGGG TCAGTTGCAC 1141 GACTATCAGC TTCGATTTCC CGGGA //
Primer: CMV-Forward Sequence file: RDB08955_A2Kfd.seq
>04389_08955_A2Kf_CMV-Forward_F04_16.ab1     1 ATTTTCCACC TTCGGCCCAA TTGACGGCAA ATGGGCGGTA GGCGTGTACG GTGGGGAGGT    61 CTATATAAGC AGCGCGTTTT GCCTGTACTG GGTCTCTCTG GTTAGACCAG ATCTGAGCCT   121 GGGAGCTCTC TGGCTAACTA GGGAACCCAC TGCTTAAGCC TCAATAAAGC TTGCCTTGAG   181 TGCTTCAAGT AGTGTGTGCC CGTCTGTTGT GTGACTCTGG TAACTAGAGA TCCCTCAGAC   241 CCTTTTAGTC AGTGTGGAAA ATCTCTAGCA GTGGCGCCCG AACAGGGACT TGAAAGCGAA   301 AGGGAAACCA GAGGAGCTCT CTCGACGCAG GACTCGGCTT GCTGAAGCGC GCACGGCAAG   361 AGGCGAGGGG CGGCGACTGG TGAGTACGCC AAAAATTTTG ACTAGCGGAG GCTAGAAGGA   421 GAGAGATGGG TGCGAGAGCG TCAGTATTAA GCGGGGGAGA ATTAGATCGC GATGGGAAAA   481 AATTCGGTTA AGGCCAGGGG GAAAGAAAAA ATATAAATTA AAACATATAG TATGGGCAAG   541 CAGGGAGCTA GAACGATTCG CAGTTAATCC TGGCCTGTTA GAAACATCAG AAGGCTGTAG   601 ACAAATACTG GGACAGCTAC AACCATCCCT TCAGACAGGA TCAGAAGAAC TTAGATCATT   661 ATATAATACA GTAGCAACCC TCTATTGTGT GCATCAAAGG ATAGAGATAA AAGACACCAA   721 GGAAGCTTTA GACAAGATAG AGGAAGAGCA AAACAAAAGT AAGACCACCG CACAGCAAGG   781 GCCGCTGATC TTCAGACCTG GAGGAGGAGA TATGAGGGAC AATTGGAGAA GTGAATTATA   841 TAAATATAAA AGTAGTAAAA ATTGAACCAT TAGGAGTAGC ACCCACCAAG GCAAAGAGAA   901 GAGTGGTGCA GAAGAGAAAA AAAGAGCAGT GGGGAATAGG AGCTTTGTTT CCTTTGGGGT   961 TCTTGGGAGC AGCAGGAAGC ACTATGGGGC GCAGCGTCAA TGACGCTGAC GGTACAGGCC  1021 AAGACAATTA ATTGTCTGGT TATAGTGCAG CAGCAGAACA ATTTTGCCTG AGGGCTATTT  1081 GAGGCGCAAC AGCAATTCTT GTTGCAACTC ACAGTCCTGG GCAATCAGGC AAGCTCCAAG 1141 ACAGATCCTG TCTGTTGGAA AGGAGAT //
Primer: BGH_rev Sequence file: RDB08955_A2Kfe.seq
>04389_08955_A2Kf_BGHrev_G04_19.ab1     1 CTTATTCCGG GGCCCTGCTA GAGAATTTTC CACACTGACT AAAGGGTCTG AGGGATCTCT    61 AGTTACCAGA GTCACACAAC AGACGGGCAC ACACTACTTG AAGCACTCAA GGCAAGCTTT   121 ATTGAGGCTT AAGCAGTGGG TTCCCTAGTT AGCCAGAGAG CTCCCAGGCT CAGATCTGGT   181 CTAACCAGAG AGACCCAGTA CAGTCCGGAT GCAGCTCTCG GGCCATGTGA TGAAATGCTA   241 GGCGGCTGTC AAACCTCCAC TCTAATACTT CTCTCTCCGG GTCATCCATC CCATGCAGGC   301 TCACAGGGTG TAACAAGCGG GTGTTCTCTC CTTCATTGGC TTCTTCTACC TTCTCTTGCT   361 CAACTGGTAC TAGCTTGTAG CACCATCCAA AGGTCAGTGG ATATCTGATC CCTGGCCCTG   421 GTGTGTAGTT CTGCCAATCA GGGAAGTAGC CTTGTGTGTG GTAGATCCAC AGATCAAGGA   481 TATCTTGTCT TCGTTGGGAG TGAATTAGCC CTTCCAGTCC CCCCTTTTCT TTTAAAAAGT   541 GGCTAAGATC TACAGCTGCC TTGTAAGTCA TTGGTCTTAA AGGTACCTGA GGTGTGACTG   601 GAAAACCCAC CTCCTCCTCC TCTTGTGCTT CTAGCCAGGC ACAATCAGCA TTGGTAGCTG   661 CTGTATTGCT ACTTGTGATT GCTCCATGTT TTTCTAGGTC TCGACGGTAT CGATGCGGGG   721 AGGCGGCCCA AAGGGAGATC CGACTCGTCT GAGGGCGAAG GCGAAGACGC GGAAGAGGCC   781 GCAGAGCCGG CAGCAGGCCG CGGGAAGGAA GGTCCGCTGG ATTGAGGGCC GAAGGGACGT   841 AGCAGAAGGA CGTCCCGCGC AGAATCCAGG TGGCAACACA GGCGAGCAGC CATGGAAAGG   901 ACGTCAGCTT CCCCGACAAC ACCACGGAAT TGTCAGTGCC CAACAGCCGA GCCCCTGTCC   961 AGCAGCGGGC AAAGGCAGGC GGCGATGAGT TCCGCCGTGG CAATAGGGAG GGGGAAAGCG  1021 AAAGTCCGGA AAGGAGCTGA CAGGTGGTGG CAATGCCCCA AACCAGTGGG GGGTTGCGTC  1081 AGCAACACAG TGCACACCAC GCCACGTTTG CCCTGACCAC CGGCCACACT CGTCATAAGA 1141 GACAGGCCAA CCCAGGAAAT TTTATTACCA AGGAAGGAGA A //
Primer: SV40pro_F_V2 Sequence file: RDB08955_A2Kff.seq
>04389_08955_A2Kf_SV40pro_F_V2_H04_22.ab1     1 GTCCACCGTT TTTTGGGAGG CCTAGGGCTT TTGCAAAAAG CTCCCGGGAG CTTGTATATC    61 CATTTTCGGA TCTGATCAGC ACGTGTTGAC AATTAATCAT CGGCATAGTA TATCGGCATA   121 GTATAATACG ACAAGGTGAG GAACTAAACC ATGGCCAAGT TGACCAGTGC CGTTCCGGTG   181 CTCACCGCGC GCGACGTCGC CGGAGCGGTC GAGTTCTGGA CCGACCGGCT CGGGTTCTCC   241 CGGGACTTCG TGGAGGACGA CTTCGCCGGT GTGGTCCGGG ACGACGTGAC CCTGTTCATC   301 AGCGCGGTCC AGGACCAGGT GGTGCCGGAC AACACCCTGG CCTGGGTGTG GGTGCGCGGC   361 CTGGACGAGC TGTACGCCGA GTGGTCGGAG GTCGTGTCCA CGAACTTCCG GGACGCCTCC   421 GGGCCGGCCA TGACCAAGAT CGGCGAGCAG CCGTGGGGGC GGGAGTTCGC CCTGCGCGAC   481 CCGGCCGGCA ACTGCGTGCA CTTCGTGGCC GAGGAGCAGG ACTGACACGT GCTACGAGAT   541 TTCGATTCCA CCGCCGCCTT CTATGAAAGG TTGGGCTTCG GAATCGTTTT CCGGGACGCC   601 GGCTGGATGA TCCTCCAGCG CGGGGATCTC ATGCTGGAGT TCTTCGCCCA CCCCAACTTG   661 TTTATTGCAG CTTATAATGG TTACAAATAA AGCAATAGCA TCACAAATTT CACAAATAAA   721 GCATTTTTTT CACTGCATTC TAGTTGTGGT TTGTCCAAAC TCATCAATGT ATCTTATCAT   781 GTCTGTATAC CGTCGACCTC TAGCTAGAGC TTGGCGTAAA TCATGGTCAT AAGCTGTTTC   841 CTGTGTGAAA ATTGTTATCC GCTCACAAAT TCCACACAAC ATACGAGCCG GGAAAGCATA   901 AAGTGTAAAG CCTGGGGGTG CCCTAATGAG TGAGCTAACT CACATTAATT TGCGTTGCGC   961 TCACTGCCCC GCTTTCCAGT CGGGGAAACC TGTCGTGCCA GCTGCATTTA ATGAATCGGG  1021 CCAAACGCGC GGGGAGAAGG CGGGTTTTGC GTATTGGGCG CTCTTCCCGC TTCCTTCGCT  1081 CACTGACTCG CTGCGCTTCG GTCGTTCGGC TGCGGCGAAG CGGGTATCAG CTCACCTCAA 1141 GGGCGGTAAT ACCGGGTTAT TCCAACAGAA AATTCTAGAG GGGGAATTTA TACCGGG //
Primer: EF1a-N_R Sequence file: RDB08955_A2Kfg.seq
>04389_08955_A9Cq_EF1a-N_R_D01_10_ABI24.ab1     1 ATCTCGGGGG GTCTGTGGGC GATGTGCGCT CTGCCCACTG ACGGGCACCG GAGCCTCACG    61 AAGCTTGAAT TAATTCAAAG TGAATTTCTG CTGTCCCTGT AATAAACCCG AAAATTTTGA   121 ATTTTTGTAA TTTGTTTTTG TAATTCTTTA GTTTGTATGT CTGTTGCTAT TATGTCTACT   181 ATTCTTTCCC CTGCACTGTA CCCCCCAATC CCCCCTTTTC TTTTAAAATT GTGGATGAAT   241 ACTGCCATTT GTAGATCCGT TCACTAATCG AATGGATCTG TCTCTGTCTC TCTCTCCACC   301 TTCTTCTTCT ATTCCTTCGG GCCTGTCGGG TCCCCTCGGG GTTGGGAGGT GGGTCTGAAA   361 CGATAATGGT GAATATCCCT GCCTAACTCT ATTCACTATA GAAAGTACAG CAAAAACTAT   421 TCTTAAACCT ACCAAGCCTC CTACTATCAT TATGAATAAT TTTATATACC ACAGCCAATT   481 TGTTATGTTA AACCAATTCC ACAAACTTGC CCATTTATCT AATTCCAATA ATTCTTGTTC   541 ATTCTTTTCT TGCTGGTTTT GCGATTCTTC AATTAAGGAG TGTATTAAGC TTGTGTAATT   601 GTTAATTTCT CTGTCCCACT CCATCCAGGT CGTGTGATTC CAAATCTGTT CCAGAGATTT   661 ATTACTCCAA CTAGCATTCC AAGGCACAGC AGTGGTGCAA ATGAGTTTTC CAGAGCAACC   721 CCAAATCCCC AGGAGCTGTT GATCCTTTAG GTATCTTTCC ACAGCCAGGA TTCTTGCCTG   781 GAGCTGCTTG ATGCCCCAGA CTGTGAGTTG CAACAGATGC TGTTGCGCCT CAATAGCCCT   841 CAGCAAATTG TTCTGCTGCT GCACTATACC AGACAATAAT TGTCTGGCCT GTACCGTCAG   901 CGTCATTGAC GCTGCGCCCA TAGTGCTTCC TGCTGCTCCC AAGAACCCAA AGGAACAAAG   961 CTCCTATTCC CACTGCTCTT TTTTCTCTCT GCACCACTCT TCTCTTTGCC CTTGGTGGGG  1021 TGCTACTTCC TATGATTCAT TTTTACTACT TTTATATTAT ATAACTCACT TCTCCAATTG 1081 TCCCTCATAT CTCCTTCTTC CAGGTCCTGA GATTCAGCCG GACCCCATGC //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.05.22

GNP_filter3_RDBDEP_html_230512.pl