RDB04389 - CSII-EF-RfA-IRES2-Venus

Backbone vector plasmid of lentivirus construct driven by EF-1 alpha promoter by Gateway(R) cloning with Venus fluorescent protein marker.

Distribution information

Sequence	RDB04389z.seq
Remarks, protocol and/or map (pdf)	RDB04389.pdf

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer. Additional terms and conditions: RECIPIENT is requested to obtain written consent of Dr. Atsushi Miyawaki of the RIKEN CBS regarding Venus fluorescent protein.
Ordering	Please visit Ordering instruction.[link] Order form [Credit Card Payment] [Bank Transfer Payment] Material Transfer Agreement (MTA, for use for not-for-profit academic purpose)[Word] Approval form [Word] by Dr. Atsushi Miyawaki
Remarks	Remember that you will be working with samples containing infectious virus. Use Survival2 (Invitrogen) or equivalent to amplify this clone. Please obtain written approval of Dr. Atsushi Miyawaki.

提供案内 (日本国内)

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提供条件	本件研究材料は、非営利機関の非営利学術研究に限って提供する。本件研究材料を利用した研究結果等を発表する際は、本件研究材料が故三好浩之博士により開発されたことを明示する。付加的提供条件：利用者は、Venus蛍光タンパク質について理化学研究所CBS 宮脇敦史博士から、書面による提供承諾を得ること。
提供依頼	手続きの詳細は、「レンチウイルスベクターの提供申し込み」をご覧ください。提供依頼書 [Word] 提供同意書 (非営利機関による非営利学術研究用)[Word] 遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。宮脇敦史博士による提供承諾書 [Word]
備考	このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。このクローンの増殖にはSurvival2 (Invitrogen)あるいは同等の宿主を使用してください。宮脇敦史博士の書面による提供承諾が必要です。

References and tips

QC test results

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB04389	CSII-EF-RfA-IRES2-Venus	DNA solution

Featured content	Lentivirus Vector (English text)
Featured content	Lentivirus Vector (Japanese text)

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check

icon as well as clone information before placing your order.

user_report	Watanabe, H., Flexible and Accurate Substrate Processing with Distinct Presenilin/gamma-Secretases in Human Cortical Neurons eNeuro 8 (2): ENEURO.0500-20.2021 (2021). PMID 33608391.
user_report	Yoshitomi, H., Human Sox4 facilitates the development of CXCL13-producing helper T cells in inflammatory environments. Nat. Commun. 9 (1): 3762 (2018). PMID 30232328.
user_report	Naka, H., Requirement for COUP-TFI and II in the temporal specification of neural stem cells in CNS development. Nat. Neurosci. 11 (9): 1014-1023 (2008). PMID 19160499.

Primer: pAxEF_F1 Sequence file: RDB08955_A2Kfa.seq
>04389_08955_A2Kf_pAxEFF1_C05_08.ab1 1 ATTTTTTTCT TCATTTCAGG TGTCGTGACA CGCTACCGGT CTCGAGAATT CTGCAGCGGC 61 CATCACAAGT TTGTACAAAA AAGCTGAACG AGAAACGTAA AATGATATAA ATATCAATAT 121 ATTAAATTAG ATTTTGCATA AAAAACAGAC TACATAATAC TGTAAAACAC AACATATCCA 181 GTCACTATGG CGGCCGCATT AGGCACCCCA GGCTTTACAC TTTATGCTTC CGGCTCGTAT 241 AATGTGTGGA TTTTGAGTTA GGATCCGTCG AGATTTTCAG GAGCTAAGGA AGCTAAAATG 301 GAGAAAAAAA TCACTGGATA TACCACCGTT GATATATCCC AATGGCATCG TAAAGAACAT 361 TTTGAGGCAT TTCAGTCAGT TGCTCAATGT ACCTATAACC AGACCGTTCA GCTGGATATT 421 ACGGCCTTTT TAAAGACCGT AAAGAAAAAT AAGCACAAGT TTTATCCGGC CTTTATTCAC 481 ATTCTTGCCC GCCTGATGAA TGCTCATCCG GAATTCCGTA TGGCAATGAA AGACGGTGAG 541 CTGGTGATAT GGGATAGTGT TCACCCTTGT TACACCGTTT TCCATGAGCA AACTGAAACG 601 TTTTCATCGC TCTGGAGTGA ATACCACGAC GATTTCCGGC AGTTTCTACA CATATATTCG 661 CAAGATGTGG CGTGTTACGG TGAAAACCTG GCCTATTTCC CTAAAGGGTT TATTGAGAAT 721 ATGTTTTTCG TCTCAGCCAA TCCCTGGGTG AGTTTCACCA GTTTTGATTT AAACGTGGCC 781 AATATGGACA ACTTCTTCGC CCCCGTTTTC ACCATGGGCA AATATTATAC GCAAGGCGAC 841 AAGGTGCTGA TGCCGCTGGC GATTCAGGTT CATCATGCCG TTTGTGATGG CTTCCATGTC 901 GGCAGAATGC TTAATGAATT ACAACAGTAC TGCGATGAGT GGCAGGCGGG GCGTAAACGC 961 GTGGATCCGG CTTACTAAAA GCCAGATAAC AGTATGCGTA TTTGCGCGCT GATTTTTGCG 1021 GGTATAAGAA TATATACTGA TATGTAATAC CCGAAGTATG TCAAAAAAGA GGTATGCTAT 1081 GAAAGCAGCG TATTACAGTG ACAGTTGAAC AGCGAACAGG CTATCAGTGG CTCAGCCATA 1141 ATATGATGGT CAATATCTCC GGGTCTTGGG TAAGCCACAA CCCAATGGCC CA //
Primer: IRES-R Sequence file: RDB08955_A2Kfb.seq
>04389_08955_A2Kf_IRES-R_D04_10.ab1 1 GCAAATGCCG GGCTTCGGGC AGTAACGTTA GGGGGGGGGG AGGGAGAGGG GCGGATCCGC 61 GGCCATCACC ACTTTGTACA AGAAAGCTGA ACGAGAAACG TAAAATGATA TAAATATCAA 121 TATATTAAAT TAGATTTTGC ATAAAAAACA GACTACATAA TACTGTAAAA CACAACATAT 181 CCAGTCACTA TGGTCGACCT GCAGACTGGC TGTGTATAAG GGAGCCTGAC ATTTATATTC 241 CCCAGAACAT CAGGTTAATG GCGTTTTTGA TGTCATTTTC GCGGTGGCTG AGATCAGCCA 301 CTTCTTCCCC GATAACGGAG ACCGGCACAC TGGCCATATC GGTGGTCATC ATGCGCCAGC 361 TTTCATCCCC GATATGCACC ACCGGGTAAA GTTCACGGGA GACTTTATCT GACAGCAGAC 421 GTGCACTGGC CAGGGGGATC ACCATCCGTC GCCCGGGCGT GTCAATAATA TCACTCTGTA 481 CATCCACAAA CAGACGATAA CGGCTCTCTC TTTTATAGGT GTAAACCTTA AACTGCATTT 541 CACCAGCCCC TGTTCTCGTC AGCAAAAGAG CCGTTCATTT CAATAAACCG GGCGACCTCA 601 GCCATCCCTT CCTGATTTTC CGCTTTCCAG CGTTCGGCAC GCAGACGACG GGCTTCATTC 661 TGCATGGTTG TGCTTACCAG ACCGGAGATA TTGACATCAT ATATGCCTTG AGCAACTGAT 721 AGCTGTCGCT GTCAACTGTC ACTGTAATAC GCTGCTTCAT AGCATACCTC TTTTTGACAT 781 ACTTCGGGTA TACATATCAG TATATATTCT TATACCGCAA AAATCAGCGC GCAAATACGC 841 ATACTGTTAT CTGGCTTTTA GTAAGCCGGA TCCACGCGTT TACGCCCCGC CCTGCCACTC 901 ATCGCAGTAC TGTTGTAATT CATTAAGCAT TCTGCCGACA TGGAAGCCAT CACAAACGGC 961 ATGATGAACC TGAATCGCCA GCGGCATCAG CACCTTGTCG CCCTTGCGTA TAATATTTGC 1021 CCATGGGTGA AAACGGGGGG GCGAAAGAAG TTGTTCCATA TTGGCCACGT TTTAAATCAA 1081 AACTGTGAAC TCACCCAGGA TTGGCTGGAG ACGAAACCAT ATTCTCAATA AACCGTTTAG 1141 GGAAAATAGG CCCAGGTTTT T //
Primer: IRES-F Sequence file: RDB08955_A2Kfc.seq
>04389_08955_A2Kf_IRES-F_E04_13.ab1 1 CGAGCGTTTA ATGTGTTTAG TCGAGGTTAA AAAAACGTCT AGGCCCCCCG AACCACGGGG 61 ACGTGGTTTT CCTTTGAAAA ACACGATGAT AATATGGCCA CAACCATGGT GAGCAAGGGC 121 GAGGAGCTGT TCACCGGGGT GGTGCCCATC CTGGTCGAGC TGGACGGCGA CGTAAACGGC 181 CACAAGTTCA GCGTGTCCGG CGAGGGCGAG GGCGATGCCA CCTACGGCAA GCTGACCCTG 241 AAGCTGATCT GCACCACCGG CAAGCTGCCC GTGCCCTGGC CCACCCTCGT GACCACCCTG 301 GGCTACGGCC TGCAGTGCTT CGCCCGCTAC CCCGACCACA TGAAGCAGCA CGACTTCTTC 361 AAGTCCGCCA TGCCCGAAGG CTACGTCCAG GAGCGCACCA TCTTCTTCAA GGACGACGGC 421 AACTACAAGA CCCGCGCCGA GGTGAAGTTC GAGGGCGACA CCCTGGTGAA CCGCATCGAG 481 CTGAAGGGCA TCGACTTCAA GGAGGACGGC AACATCCTGG GGCACAAGCT GGAGTACAAC 541 TACAACAGCC ACAACGTCTA TATCACCGCC GACAAGCAGA AGAACGGCAT CAAGGCCAAC 601 TTCAAGATCC GCCACAACAT CGAGGACGGC GGCGTGCAGC TCGCCGACCA CTACCAGCAG 661 AACACCCCCA TCGGCGACGG CCCCGTGCTG CTGCCCGACA ACCACTACCT GAGCTACCAG 721 TCCGCCCTGA GCAAAGACCC CAACGAGAAG CGCGATCACA TGGTCCTGCT GGAGTTCGTG 781 ACCGCCGCCG GGATCACTCT CGGCATGGAC GAGCTGTACA AGTAAGAATT CAAGGCCTCT 841 CGAGCCTCTA GAGTTAACAT CGAGGGATCA AGCTTATCGA TAATCAACCT CTGGATTACA 901 AAATTTGTGA AAGATTGACT GGTATTCTTA ACTATGTTGC TCCTTTTACG CTATGTGGAT 961 ACGCTGCTTT AATGCCTTTG TATCATGCTA TTGCTTCCCG TATGGCTTTC ATTTTCTCCT 1021 CCCTTGTATA AATCCTGGAT GCTGTCTCTT TATGAGAGTT GTGGCCCGAT GTCAGCAACG 1081 TGACGTGATG TGCACTGTGT TTGCCTGGAC GGCACCCCAA CTGATGGGGG TCAGTTGCAC 1141 GACTATCAGC TTCGATTTCC CGGGA //
Primer: CMV-Forward Sequence file: RDB08955_A2Kfd.seq
>04389_08955_A2Kf_CMV-Forward_F04_16.ab1 1 ATTTTCCACC TTCGGCCCAA TTGACGGCAA ATGGGCGGTA GGCGTGTACG GTGGGGAGGT 61 CTATATAAGC AGCGCGTTTT GCCTGTACTG GGTCTCTCTG GTTAGACCAG ATCTGAGCCT 121 GGGAGCTCTC TGGCTAACTA GGGAACCCAC TGCTTAAGCC TCAATAAAGC TTGCCTTGAG 181 TGCTTCAAGT AGTGTGTGCC CGTCTGTTGT GTGACTCTGG TAACTAGAGA TCCCTCAGAC 241 CCTTTTAGTC AGTGTGGAAA ATCTCTAGCA GTGGCGCCCG AACAGGGACT TGAAAGCGAA 301 AGGGAAACCA GAGGAGCTCT CTCGACGCAG GACTCGGCTT GCTGAAGCGC GCACGGCAAG 361 AGGCGAGGGG CGGCGACTGG TGAGTACGCC AAAAATTTTG ACTAGCGGAG GCTAGAAGGA 421 GAGAGATGGG TGCGAGAGCG TCAGTATTAA GCGGGGGAGA ATTAGATCGC GATGGGAAAA 481 AATTCGGTTA AGGCCAGGGG GAAAGAAAAA ATATAAATTA AAACATATAG TATGGGCAAG 541 CAGGGAGCTA GAACGATTCG CAGTTAATCC TGGCCTGTTA GAAACATCAG AAGGCTGTAG 601 ACAAATACTG GGACAGCTAC AACCATCCCT TCAGACAGGA TCAGAAGAAC TTAGATCATT 661 ATATAATACA GTAGCAACCC TCTATTGTGT GCATCAAAGG ATAGAGATAA AAGACACCAA 721 GGAAGCTTTA GACAAGATAG AGGAAGAGCA AAACAAAAGT AAGACCACCG CACAGCAAGG 781 GCCGCTGATC TTCAGACCTG GAGGAGGAGA TATGAGGGAC AATTGGAGAA GTGAATTATA 841 TAAATATAAA AGTAGTAAAA ATTGAACCAT TAGGAGTAGC ACCCACCAAG GCAAAGAGAA 901 GAGTGGTGCA GAAGAGAAAA AAAGAGCAGT GGGGAATAGG AGCTTTGTTT CCTTTGGGGT 961 TCTTGGGAGC AGCAGGAAGC ACTATGGGGC GCAGCGTCAA TGACGCTGAC GGTACAGGCC 1021 AAGACAATTA ATTGTCTGGT TATAGTGCAG CAGCAGAACA ATTTTGCCTG AGGGCTATTT 1081 GAGGCGCAAC AGCAATTCTT GTTGCAACTC ACAGTCCTGG GCAATCAGGC AAGCTCCAAG 1141 ACAGATCCTG TCTGTTGGAA AGGAGAT //
Primer: BGH_rev Sequence file: RDB08955_A2Kfe.seq
>04389_08955_A2Kf_BGHrev_G04_19.ab1 1 CTTATTCCGG GGCCCTGCTA GAGAATTTTC CACACTGACT AAAGGGTCTG AGGGATCTCT 61 AGTTACCAGA GTCACACAAC AGACGGGCAC ACACTACTTG AAGCACTCAA GGCAAGCTTT 121 ATTGAGGCTT AAGCAGTGGG TTCCCTAGTT AGCCAGAGAG CTCCCAGGCT CAGATCTGGT 181 CTAACCAGAG AGACCCAGTA CAGTCCGGAT GCAGCTCTCG GGCCATGTGA TGAAATGCTA 241 GGCGGCTGTC AAACCTCCAC TCTAATACTT CTCTCTCCGG GTCATCCATC CCATGCAGGC 301 TCACAGGGTG TAACAAGCGG GTGTTCTCTC CTTCATTGGC TTCTTCTACC TTCTCTTGCT 361 CAACTGGTAC TAGCTTGTAG CACCATCCAA AGGTCAGTGG ATATCTGATC CCTGGCCCTG 421 GTGTGTAGTT CTGCCAATCA GGGAAGTAGC CTTGTGTGTG GTAGATCCAC AGATCAAGGA 481 TATCTTGTCT TCGTTGGGAG TGAATTAGCC CTTCCAGTCC CCCCTTTTCT TTTAAAAAGT 541 GGCTAAGATC TACAGCTGCC TTGTAAGTCA TTGGTCTTAA AGGTACCTGA GGTGTGACTG 601 GAAAACCCAC CTCCTCCTCC TCTTGTGCTT CTAGCCAGGC ACAATCAGCA TTGGTAGCTG 661 CTGTATTGCT ACTTGTGATT GCTCCATGTT TTTCTAGGTC TCGACGGTAT CGATGCGGGG 721 AGGCGGCCCA AAGGGAGATC CGACTCGTCT GAGGGCGAAG GCGAAGACGC GGAAGAGGCC 781 GCAGAGCCGG CAGCAGGCCG CGGGAAGGAA GGTCCGCTGG ATTGAGGGCC GAAGGGACGT 841 AGCAGAAGGA CGTCCCGCGC AGAATCCAGG TGGCAACACA GGCGAGCAGC CATGGAAAGG 901 ACGTCAGCTT CCCCGACAAC ACCACGGAAT TGTCAGTGCC CAACAGCCGA GCCCCTGTCC 961 AGCAGCGGGC AAAGGCAGGC GGCGATGAGT TCCGCCGTGG CAATAGGGAG GGGGAAAGCG 1021 AAAGTCCGGA AAGGAGCTGA CAGGTGGTGG CAATGCCCCA AACCAGTGGG GGGTTGCGTC 1081 AGCAACACAG TGCACACCAC GCCACGTTTG CCCTGACCAC CGGCCACACT CGTCATAAGA 1141 GACAGGCCAA CCCAGGAAAT TTTATTACCA AGGAAGGAGA A //
Primer: SV40pro_F_V2 Sequence file: RDB08955_A2Kff.seq
>04389_08955_A2Kf_SV40pro_F_V2_H04_22.ab1 1 GTCCACCGTT TTTTGGGAGG CCTAGGGCTT TTGCAAAAAG CTCCCGGGAG CTTGTATATC 61 CATTTTCGGA TCTGATCAGC ACGTGTTGAC AATTAATCAT CGGCATAGTA TATCGGCATA 121 GTATAATACG ACAAGGTGAG GAACTAAACC ATGGCCAAGT TGACCAGTGC CGTTCCGGTG 181 CTCACCGCGC GCGACGTCGC CGGAGCGGTC GAGTTCTGGA CCGACCGGCT CGGGTTCTCC 241 CGGGACTTCG TGGAGGACGA CTTCGCCGGT GTGGTCCGGG ACGACGTGAC CCTGTTCATC 301 AGCGCGGTCC AGGACCAGGT GGTGCCGGAC AACACCCTGG CCTGGGTGTG GGTGCGCGGC 361 CTGGACGAGC TGTACGCCGA GTGGTCGGAG GTCGTGTCCA CGAACTTCCG GGACGCCTCC 421 GGGCCGGCCA TGACCAAGAT CGGCGAGCAG CCGTGGGGGC GGGAGTTCGC CCTGCGCGAC 481 CCGGCCGGCA ACTGCGTGCA CTTCGTGGCC GAGGAGCAGG ACTGACACGT GCTACGAGAT 541 TTCGATTCCA CCGCCGCCTT CTATGAAAGG TTGGGCTTCG GAATCGTTTT CCGGGACGCC 601 GGCTGGATGA TCCTCCAGCG CGGGGATCTC ATGCTGGAGT TCTTCGCCCA CCCCAACTTG 661 TTTATTGCAG CTTATAATGG TTACAAATAA AGCAATAGCA TCACAAATTT CACAAATAAA 721 GCATTTTTTT CACTGCATTC TAGTTGTGGT TTGTCCAAAC TCATCAATGT ATCTTATCAT 781 GTCTGTATAC CGTCGACCTC TAGCTAGAGC TTGGCGTAAA TCATGGTCAT AAGCTGTTTC 841 CTGTGTGAAA ATTGTTATCC GCTCACAAAT TCCACACAAC ATACGAGCCG GGAAAGCATA 901 AAGTGTAAAG CCTGGGGGTG CCCTAATGAG TGAGCTAACT CACATTAATT TGCGTTGCGC 961 TCACTGCCCC GCTTTCCAGT CGGGGAAACC TGTCGTGCCA GCTGCATTTA ATGAATCGGG 1021 CCAAACGCGC GGGGAGAAGG CGGGTTTTGC GTATTGGGCG CTCTTCCCGC TTCCTTCGCT 1081 CACTGACTCG CTGCGCTTCG GTCGTTCGGC TGCGGCGAAG CGGGTATCAG CTCACCTCAA 1141 GGGCGGTAAT ACCGGGTTAT TCCAACAGAA AATTCTAGAG GGGGAATTTA TACCGGG //
Primer: EF1a-N_R Sequence file: RDB08955_A2Kfg.seq
>04389_08955_A9Cq_EF1a-N_R_D01_10_ABI24.ab1 1 ATCTCGGGGG GTCTGTGGGC GATGTGCGCT CTGCCCACTG ACGGGCACCG GAGCCTCACG 61 AAGCTTGAAT TAATTCAAAG TGAATTTCTG CTGTCCCTGT AATAAACCCG AAAATTTTGA 121 ATTTTTGTAA TTTGTTTTTG TAATTCTTTA GTTTGTATGT CTGTTGCTAT TATGTCTACT 181 ATTCTTTCCC CTGCACTGTA CCCCCCAATC CCCCCTTTTC TTTTAAAATT GTGGATGAAT 241 ACTGCCATTT GTAGATCCGT TCACTAATCG AATGGATCTG TCTCTGTCTC TCTCTCCACC 301 TTCTTCTTCT ATTCCTTCGG GCCTGTCGGG TCCCCTCGGG GTTGGGAGGT GGGTCTGAAA 361 CGATAATGGT GAATATCCCT GCCTAACTCT ATTCACTATA GAAAGTACAG CAAAAACTAT 421 TCTTAAACCT ACCAAGCCTC CTACTATCAT TATGAATAAT TTTATATACC ACAGCCAATT 481 TGTTATGTTA AACCAATTCC ACAAACTTGC CCATTTATCT AATTCCAATA ATTCTTGTTC 541 ATTCTTTTCT TGCTGGTTTT GCGATTCTTC AATTAAGGAG TGTATTAAGC TTGTGTAATT 601 GTTAATTTCT CTGTCCCACT CCATCCAGGT CGTGTGATTC CAAATCTGTT CCAGAGATTT 661 ATTACTCCAA CTAGCATTCC AAGGCACAGC AGTGGTGCAA ATGAGTTTTC CAGAGCAACC 721 CCAAATCCCC AGGAGCTGTT GATCCTTTAG GTATCTTTCC ACAGCCAGGA TTCTTGCCTG 781 GAGCTGCTTG ATGCCCCAGA CTGTGAGTTG CAACAGATGC TGTTGCGCCT CAATAGCCCT 841 CAGCAAATTG TTCTGCTGCT GCACTATACC AGACAATAAT TGTCTGGCCT GTACCGTCAG 901 CGTCATTGAC GCTGCGCCCA TAGTGCTTCC TGCTGCTCCC AAGAACCCAA AGGAACAAAG 961 CTCCTATTCC CACTGCTCTT TTTTCTCTCT GCACCACTCT TCTCTTTGCC CTTGGTGGGG 1021 TGCTACTTCC TATGATTCAT TTTTACTACT TTTATATTAT ATAACTCACT TCTCCAATTG 1081 TCCCTCATAT CTCCTTCTTC CAGGTCCTGA GATTCAGCCG GACCCCATGC //