Resource data sheet
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CSII-EF-RfA-IRES2-Venus

Shuttle vector to generate recombinant Lentiviral vector expressing venus GFP

Catalog number RDB04389
Resource name CSII-EF-RfA-IRES2-Venus
Clone info. Shuttle vector to generate recombinant Lentiviral vector expressing venus GFP
Use DB3.1 (Invitrogen) or equivalent to amplify this clone. Commonly requested with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394).
Vector backbone Plasmid
Selectable markers Amp^r
Gene/insert name Aequorea victoria GFP cDNA
Originator Miyoshi, Hiroyuki |

Distribution information

CLICK here for plasmid list and map. [link]
Forms for plasmid request [in Japanese] [in English]
Terms and conditions for distribution The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose.
(Japanese text [open/close])
Remarks ((Remarks)) ((Remarks)) Use DB3.1(Invitrogen) or equivalent to amplify this clone.
(Japanese text [open/close])

Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB08955_A2Kfp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxEF_F1 RDB08955_A2Kfa.seq
>04389_08955_A2Kf_IRES-F_H08_23.ab1
    1 CCTCCCTTTA ATGTGTTTAG TCGAGGTTAA AAAAACGTCT AGGCCCCCCG AACCACGGGG
   61 ACGTGGTTTT CCTTTGAAAA ACACGATGAT AATATGGCCA CAACCATGGT GAGCAAGGGC
  121 GAGGAGCTGT TCACCGGGGT GGTGCCCATC CTGGTCGAGC TGGACGGCGA CGTAAACGGC
  181 CACAAGTTCA GCGTGTCCGG CGAGGGCGAG GGCGATGCCA CCTACGGCAA GCTGACCCTG
  241 AAGCTGATCT GCACCACCGG CAAGCTGCCC GTGCCCTGGC CCACCCTCGT GACCACCCTG
  301 GGCTACGGCC TGCAGTGCTT CGCCCGCTAC CCCGACCACA TGAAGCAGCA CGACTTCTTC
  361 AAGTCCGCCA TGCCCGAAGG CTACGTCCAG GAGCGCACCA TCTTCTTCAA GGACGACGGC
  421 AACTACAAGA CCCGCGCCGA GGTGAAGTTC GAGGGCGACA CCCTGGTGAA CCGCATCGAG
  481 CTGAAGGGCA TCGACTTCAA GGAGGACGGC AACATCCTGG GGCACAAGCT GGAGTACAAC
  541 TACAACAGCC ACAACGTCTA TATCACCGCC GACAAGCAGA AGAACGGCAT CAAGGCCAAC
  601 TTCAAGATCC GCCACAACAT CGAGGACGGC GGCGTGCAGC TCGCCGACCA CTACCAGCAG
  661 AACACCCCCA TCGGCGACGG CCCCGTGCTG CTGCCCGACA ACCACTACCT GAGCTACCAG
  721 TCCGCCCTGA GCAAAGACCC CAACGAGAAG CGCGATCACA TGGTCCTGCT GGAGTTCGTG
  781 ACCGCCGCCG GGATCACTCT CGGCATGGAC GAGCTGTACA AGTAAGAATT CAAGGCCTCT
  841 CGAGCCTCTA GAGTTAACAT CGAGGGATCA AGCTTATCGA TAATCAACCT CTGGATTACA
  901 AAATTTGTGA AAGATTGACT GGTATTCTTA ACTATGTTGC TCCTTTTACG CTATGTGGAT
  961 ACGCTGCTTT AATGCCTTTG TATCATGCTA TTGCTTCCCG TATGGCTTTC ATTTTTCTCC
 1021 CTCCCTTGTA TAAATCCTGG ATTGCCTGTC TCTTTATGAA GGAGTTTGTG GGCCCGTTGT
 1081 CAGGCAAACG TGGCGTGTGT GCACTGTGAT TGGCCTGAAC GCACCCCCCA CTGGTTGGGG
1141 GGCCAAATTT GATCAC
//
Primer: IRES-R RDB08955_A2Kfb.seq
>04389_08955_A2Kf_IRES-R_G08_20.ab1
    1 GAATAACCGG CCTTCGGCAG TAACGTTAGG GGGGGGGGAG GGAGAGGGGC GGATCCGCGG
   61 CCATCACCAC TTTGTACAAG AAAGCTGAAC GAGAAACGTA AAATGATATA AATATCAATA
  121 TATTAAATTA GATTTTGCAT AAAAAACAGA CTACATAATA CTGTAAAACA CAACATATCC
  181 AGTCACTATG GTCGACCTGC AGACTGGCTG TGTATAAGGG AGCCTGACAT TTATATTCCC
  241 CAGAACATCA GGTTAATGGC GTTTTTGATG TCATTTTCGC GGTGGCTGAG ATCAGCCACT
  301 TCTTCCCCGA TAACGGAGAC CGGCACACTG GCCATATCGG TGGTCATCAT GCGCCAGCTT
  361 TCATCCCCGA TATGCACCAC CGGGTAAAGT TCACGGGAGA CTTTATCTGA CAGCAGACGT
  421 GCACTGGCCA GGGGGATCAC CATCCGTCGC CCGGGCGTGT CAATAATATC ACTCTGTACA
  481 TCCACAAACA GACGATAACG GCTCTCTCTT TTATAGGTGT AAACCTTAAA CTGCATTTCA
  541 CCAGCCCCTG TTCTCGTCAG CAAAAGAGCC GTTCATTTCA ATAAACCGGG CGACCTCAGC
  601 CATCCCTTCC TGATTTTCCG CTTTCCAGCG TTCGGCACGC AGACGACGGG CTTCATTCTG
  661 CATGGTTGTG CTTACCAGAC CGGAGATATT GACATCATAT ATGCCTTGAG CAACTGATAG
  721 CTGTCGCTGT CAACTGTCAC TGTAATACGC TGCTTCATAG CATACCTCTT TTTGACATAC
  781 TTCGGGTATA CATATCAGTA TATATTCTTA TACCGCAAAA ATCAGCGCGC AAATACGCAT
  841 ACTGTTATCT GGCTTTTAGT AAGCCGGATC CACGCGTTTA CGCCCCGCCC TGCCACTCAT
  901 CGCAGTACTG TTGTAATTCA TTAAGCATTC TGCCGACATG GAAGCCATCA CAAACGGCAT
  961 GATGAACCTG AATCGCCAGC GGCATCAGCA CCTTGTCGCC TTGCGTATAA TATTTGCCCA
 1021 TGGTGAAAAC GGGGGGCGAG AGTTGTCCCA TATTGGCCAC GTTTAAATCA AAACTGGTGA
 1081 AACTCACCCA GGAATGGACC TGAGACGAAA ACAATATTCT CAATAAAACC CTTTTAAGGA
//
Primer: IRES-F RDB08955_A2Kfc.seq
>04389_08955_A2Kf_CMV-Foward_E08_14.ab1
    1 CGATCCCCTC CGGCCCCATT GACGCAAATG GGCGGTAGGC GTGTACGGTG GGAGGTCTAT
   61 ATAAGCAGCG CGTTTTGCCT GTACTGGGTC TCTCTGGTTA GACCAGATCT GAGCCTGGGA
  121 GCTCTCTGGC TAACTAGGGA ACCCACTGCT TAAGCCTCAA TAAAGCTTGC CTTGAGTGCT
  181 TCAAGTAGTG TGTGCCCGTC TGTTGTGTGA CTCTGGTAAC TAGAGATCCC TCAGACCCTT
  241 TTAGTCAGTG TGGAAAATCT CTAGCAGTGG CGCCCGAACA GGGACTTGAA AGCGAAAGGG
  301 AAACCAGAGG AGCTCTCTCG ACGCAGGACT CGGCTTGCTG AAGCGCGCAC GGCAAGAGGC
  361 GAGGGGCGGC GACTGGTGAG TACGCCAAAA ATTTTGACTA GCGGAGGCTA GAAGGAGAGA
  421 GATGGGTGCG AGAGCGTCAG TATTAAGCGG GGGAGAATTA GATCGCGATG GGAAAAAATT
  481 CGGTTAAGGC CAGGGGGAAA GAAAAAATAT AAATTAAAAC ATATAGTATG GGCAAGCAGG
  541 GAGCTAGAAC GATTCGCAGT TAATCCTGGC CTGTTAGAAA CATCAGAAGG CTGTAGACAA
  601 ATACTGGGAC AGCTACAACC ATCCCTTCAG ACAGGATCAG AAGAACTTAG ATCATTATAT
  661 AATACAGTAG CAACCCTCTA TTGTGTGCAT CAAAGGATAG AGATAAAAGA CACCAAGGAA
  721 GCTTTAGACA AGATAGAGGA AGAGCAAAAC AAAAGTAAGA CCACCGCACA GCAAGGGCCG
  781 CTGATCTTCA GACCTGGAGG AGGAGATATG AGGGACAATT GGAGAAGTGA ATTATATAAA
  841 TATAAAGTAG TAAAAATTGA ACCATTAGGA GTAGCACCCA CCAAGGCAAA GAGAAGAGTG
  901 GTGCAGAGAG AAAAAAGAGC AGTGGGAATA GGAGCTTTGT TCCTTGGGTT CTTGGGAGCA
  961 GCACGAAGCA CTATGGGCGC AGCGTCAATG ACGCTGACGG GTACAGGCCA GACAATTATT
 1021 GTCTGGTTAT AGTGCAGCAG CAGAACAAAT TTGCTGAGGG CTATTGAGGC GCAACAGCCA
 1081 TCCTATGCAA CTTCACAGGT CCTGGGGGCC ATCCAGGCCA GCTTCCAAGG CAAGAAATTC
1141 CG
//
Primer: CMV-Forward RDB08955_A2Kfd.seq
>04389_08955_A2Kf_CMV-Forward_F04_16.ab1
    1 ATTTTCCACC TTCGGCCCAA TTGACGGCAA ATGGGCGGTA GGCGTGTACG GTGGGGAGGT
   61 CTATATAAGC AGCGCGTTTT GCCTGTACTG GGTCTCTCTG GTTAGACCAG ATCTGAGCCT
  121 GGGAGCTCTC TGGCTAACTA GGGAACCCAC TGCTTAAGCC TCAATAAAGC TTGCCTTGAG
  181 TGCTTCAAGT AGTGTGTGCC CGTCTGTTGT GTGACTCTGG TAACTAGAGA TCCCTCAGAC
  241 CCTTTTAGTC AGTGTGGAAA ATCTCTAGCA GTGGCGCCCG AACAGGGACT TGAAAGCGAA
  301 AGGGAAACCA GAGGAGCTCT CTCGACGCAG GACTCGGCTT GCTGAAGCGC GCACGGCAAG
  361 AGGCGAGGGG CGGCGACTGG TGAGTACGCC AAAAATTTTG ACTAGCGGAG GCTAGAAGGA
  421 GAGAGATGGG TGCGAGAGCG TCAGTATTAA GCGGGGGAGA ATTAGATCGC GATGGGAAAA
  481 AATTCGGTTA AGGCCAGGGG GAAAGAAAAA ATATAAATTA AAACATATAG TATGGGCAAG
  541 CAGGGAGCTA GAACGATTCG CAGTTAATCC TGGCCTGTTA GAAACATCAG AAGGCTGTAG
  601 ACAAATACTG GGACAGCTAC AACCATCCCT TCAGACAGGA TCAGAAGAAC TTAGATCATT
  661 ATATAATACA GTAGCAACCC TCTATTGTGT GCATCAAAGG ATAGAGATAA AAGACACCAA
  721 GGAAGCTTTA GACAAGATAG AGGAAGAGCA AAACAAAAGT AAGACCACCG CACAGCAAGG
  781 GCCGCTGATC TTCAGACCTG GAGGAGGAGA TATGAGGGAC AATTGGAGAA GTGAATTATA
  841 TAAATATAAA AGTAGTAAAA ATTGAACCAT TAGGAGTAGC ACCCACCAAG GCAAAGAGAA
  901 GAGTGGTGCA GAAGAGAAAA AAAGAGCAGT GGGGAATAGG AGCTTTGTTT CCTTTGGGGT
  961 TCTTGGGAGC AGCAGGAAGC ACTATGGGGC GCAGCGTCAA TGACGCTGAC GGTACAGGCC
 1021 AAGACAATTA ATTGTCTGGT TATAGTGCAG CAGCAGAACA ATTTTGCCTG AGGGCTATTT
 1081 GAGGCGCAAC AGCAATTCTT GTTGCAACTC ACAGTCCTGG GCAATCAGGC AAGCTCCAAG
1141 ACAGATCCTG TCTGTTGGAA AGGAGAT
//
Primer: BGH_rev RDB08955_A2Kfe.seq
>04389_08955_A2Kf_BGHrev_G04_19.ab1
    1 CTTATTCCGG GGCCCTGCTA GAGAATTTTC CACACTGACT AAAGGGTCTG AGGGATCTCT
   61 AGTTACCAGA GTCACACAAC AGACGGGCAC ACACTACTTG AAGCACTCAA GGCAAGCTTT
  121 ATTGAGGCTT AAGCAGTGGG TTCCCTAGTT AGCCAGAGAG CTCCCAGGCT CAGATCTGGT
  181 CTAACCAGAG AGACCCAGTA CAGTCCGGAT GCAGCTCTCG GGCCATGTGA TGAAATGCTA
  241 GGCGGCTGTC AAACCTCCAC TCTAATACTT CTCTCTCCGG GTCATCCATC CCATGCAGGC
  301 TCACAGGGTG TAACAAGCGG GTGTTCTCTC CTTCATTGGC TTCTTCTACC TTCTCTTGCT
  361 CAACTGGTAC TAGCTTGTAG CACCATCCAA AGGTCAGTGG ATATCTGATC CCTGGCCCTG
  421 GTGTGTAGTT CTGCCAATCA GGGAAGTAGC CTTGTGTGTG GTAGATCCAC AGATCAAGGA
  481 TATCTTGTCT TCGTTGGGAG TGAATTAGCC CTTCCAGTCC CCCCTTTTCT TTTAAAAAGT
  541 GGCTAAGATC TACAGCTGCC TTGTAAGTCA TTGGTCTTAA AGGTACCTGA GGTGTGACTG
  601 GAAAACCCAC CTCCTCCTCC TCTTGTGCTT CTAGCCAGGC ACAATCAGCA TTGGTAGCTG
  661 CTGTATTGCT ACTTGTGATT GCTCCATGTT TTTCTAGGTC TCGACGGTAT CGATGCGGGG
  721 AGGCGGCCCA AAGGGAGATC CGACTCGTCT GAGGGCGAAG GCGAAGACGC GGAAGAGGCC
  781 GCAGAGCCGG CAGCAGGCCG CGGGAAGGAA GGTCCGCTGG ATTGAGGGCC GAAGGGACGT
  841 AGCAGAAGGA CGTCCCGCGC AGAATCCAGG TGGCAACACA GGCGAGCAGC CATGGAAAGG
  901 ACGTCAGCTT CCCCGACAAC ACCACGGAAT TGTCAGTGCC CAACAGCCGA GCCCCTGTCC
  961 AGCAGCGGGC AAAGGCAGGC GGCGATGAGT TCCGCCGTGG CAATAGGGAG GGGGAAAGCG
 1021 AAAGTCCGGA AAGGAGCTGA CAGGTGGTGG CAATGCCCCA AACCAGTGGG GGGTTGCGTC
 1081 AGCAACACAG TGCACACCAC GCCACGTTTG CCCTGACCAC CGGCCACACT CGTCATAAGA
1141 GACAGGCCAA CCCAGGAAAT TTTATTACCA AGGAAGGAGA A
//
Primer: SV40pro_F_V2 RDB08955_A2Kff.seq
>04389_08955_A2Kf_SV40pro_F_V2_H04_22.ab1
    1 GTCCACCGTT TTTTGGGAGG CCTAGGGCTT TTGCAAAAAG CTCCCGGGAG CTTGTATATC
   61 CATTTTCGGA TCTGATCAGC ACGTGTTGAC AATTAATCAT CGGCATAGTA TATCGGCATA
  121 GTATAATACG ACAAGGTGAG GAACTAAACC ATGGCCAAGT TGACCAGTGC CGTTCCGGTG
  181 CTCACCGCGC GCGACGTCGC CGGAGCGGTC GAGTTCTGGA CCGACCGGCT CGGGTTCTCC
  241 CGGGACTTCG TGGAGGACGA CTTCGCCGGT GTGGTCCGGG ACGACGTGAC CCTGTTCATC
  301 AGCGCGGTCC AGGACCAGGT GGTGCCGGAC AACACCCTGG CCTGGGTGTG GGTGCGCGGC
  361 CTGGACGAGC TGTACGCCGA GTGGTCGGAG GTCGTGTCCA CGAACTTCCG GGACGCCTCC
  421 GGGCCGGCCA TGACCAAGAT CGGCGAGCAG CCGTGGGGGC GGGAGTTCGC CCTGCGCGAC
  481 CCGGCCGGCA ACTGCGTGCA CTTCGTGGCC GAGGAGCAGG ACTGACACGT GCTACGAGAT
  541 TTCGATTCCA CCGCCGCCTT CTATGAAAGG TTGGGCTTCG GAATCGTTTT CCGGGACGCC
  601 GGCTGGATGA TCCTCCAGCG CGGGGATCTC ATGCTGGAGT TCTTCGCCCA CCCCAACTTG
  661 TTTATTGCAG CTTATAATGG TTACAAATAA AGCAATAGCA TCACAAATTT CACAAATAAA
  721 GCATTTTTTT CACTGCATTC TAGTTGTGGT TTGTCCAAAC TCATCAATGT ATCTTATCAT
  781 GTCTGTATAC CGTCGACCTC TAGCTAGAGC TTGGCGTAAA TCATGGTCAT AAGCTGTTTC
  841 CTGTGTGAAA ATTGTTATCC GCTCACAAAT TCCACACAAC ATACGAGCCG GGAAAGCATA
  901 AAGTGTAAAG CCTGGGGGTG CCCTAATGAG TGAGCTAACT CACATTAATT TGCGTTGCGC
  961 TCACTGCCCC GCTTTCCAGT CGGGGAAACC TGTCGTGCCA GCTGCATTTA ATGAATCGGG
 1021 CCAAACGCGC GGGGAGAAGG CGGGTTTTGC GTATTGGGCG CTCTTCCCGC TTCCTTCGCT
 1081 CACTGACTCG CTGCGCTTCG GTCGTTCGGC TGCGGCGAAG CGGGTATCAG CTCACCTCAA
1141 GGGCGGTAAT ACCGGGTTAT TCCAACAGAA AATTCTAGAG GGGGAATTTA TACCGGG
//

Please visit plasmidSequencing and PCR primers for primer information.

References and tips

Electronic file

References

user report Shin, J.W., Establishment of single-cell screening system for the rapid identification of transcriptional modulators involved in direct cell . Nucleic Acids Res., 40 (21): e165 (2012). PMID 22879381.
user report Naka, H., Requirement for COUP-TFI and II in the temporal specification of neural stem cells in CNS development. Nat. Neurosci. 11 (9): 1014-1023 (2008). PMID 19160499.

2017.10.23

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