RDB04388 - CSII-CMV-RfA-IRES2-Venus

Backbone vector plasmid of lentivirus construct driven by CMV promoter by Gateway(R) cloning with Venus fluorescent protein marker.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer.
Additional terms and conditions for distribution	RECIPIENT is requested to obtain written consent of Dr. Atsushi Miyawaki of the RIKEN CBS regarding Venus fluorescent protein.
Ordering	Please visit Ordering instruction.[link] Order form [Credit Card Payment] [Bank Transfer Payment] Material Transfer Agreement (MTA, for use for not-for-profit academic purpose)[Word] Approval form [Word] by Dr. Atsushi Miyawaki
Remarks	Remember that you will be working with samples containing infectious virus. Use Survival2 (Invitrogen) or equivalent to amplify this clone. Please obtain written approval of Dr. Atsushi Miyawaki.

提供案内 (日本国内)

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提供条件	本件研究材料は、非営利機関の非営利学術研究に限って提供する。本件研究材料を利用した研究結果等を発表する際は、本件研究材料が故三好浩之博士により開発されたことを明示する。
付加的提供条件	利用者は、Venus蛍光タンパク質について理化学研究所CBS 宮脇敦史博士から、書面による提供承諾を得ること。
提供依頼	手続きの詳細は、「レンチウイルスベクターの提供申し込み」をご覧ください。提供依頼書 [Word] 提供同意書 (非営利機関による非営利学術研究用)[Word] 遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。宮脇敦史博士による提供承諾書 [Word]
備考	このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。このクローンの増殖にはSurvival2 (Invitrogen)あるいは同等の宿主を使用してください。宮脇敦史博士の書面による提供承諾が必要です。

References and tips

Sequence information

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB04388	CSII-CMV-RfA-IRES2-Venus	DNA solution

Sequence	RDB04388z.seq
Remarks, protocol and/or map (pdf)	RDB04388.pdf

Featured content	Lentivirus Vector (English text)
Featured content	Lentivirus Vector (Japanese text)

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check

icon as well as clone information before placing your order.

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user_report	Kaneko, N., New neurons use Slit-Robo signaling to migrate through the glial meshwork and approach a lesion for functional regeneration. Sci. Adv. 4 (12): eaav0618 (2018). PMID 30547091.
user_report	Hashimoto, H., A novel method to generate single-cell-derived cancer-associated fibroblast clones. J. Cancer Res. Clin. Oncol. 143 (8): 1409-1419 (2017). PMID 28364361.
user_report	Ishibashi, M., CD200-positive cancer associated fibroblasts augment the sensitivity of Epidermal Growth Factor Receptor mutation-positive lung adenocarcinomas to EGFR Tyrosine kinase inhibitors. Sci. Rep. 7: 46662 (2017). PMID 28429795.
user_report	Neri, S., Cancer cell invasion driven by extracellular matrix remodeling is dependent on the properties of cancer-associated fibroblasts. J. Cancer Res. Clin. Oncol. 142 (2): 437-446 (2016). PMID 26374424.
user_report	Murakami-Tonami, Y., SGO1 is involved in the DNA damage response in MYCN-amplified neuroblastoma cells. Sci. Rep. 6: 31615 (2016). PMID 27539729.
user_report	Nakaguro, M., Nucleolar protein PES1 is a marker of neuroblastoma outcome and is associated with neuroblastoma differentiation. Cancer Sci. 106 (3): 237-243 (2015). PMID 25557119.
user_report	Neri, S., Podoplanin-expressing cancer-associated fibroblasts lead and enhance the local invasion of cancer cells in lung adenocarcinoma. Int. J. Cancer. 137 (4): 784-796 (2015). PMID 25648219.
user_report	Murakami-Tonami, Y., Inactivation of SMC2 shows a synergistic lethal response in MYCN-amplified neuroblastoma cells. Cell Cycle 13 (7): 1115-1131 (2014). PMID 24553121.
user_report	Koyama-Nasu, R., The critical role of cyclin D2 in cell cycle progression and tumorigenicity of glioblastoma stem cells. Oncogene, 32 (33): 3840-3845 (2013). PMID 22964630.
user_report	Suzuki, T.,, Reconstruction of monocyte transcriptional regulatory network accompanies monocytic functions in human fibroblasts. PLoS One, 7 (3): e33474 (2012). PMID 22428058.
user_report	Makinoshima, H., PTPRZ1 regulates calmodulin phosphorylation and tumor progression in small-cell lung carcinoma. BMC Cancer, 12: 537 (2012). PMID 23170925.
user_report	Huang, P., The neuronal differentiation factor NeuroD1 downregulates the neuronal repellent factor Slit2 expression and promotes cell motility and tumor formation of neuroblastoma. Cancer Res., 71 (8), 2938-2948 (2011). PMID 21349947.

Primer: CSII-CMV5' Region: attR1,CmR Sequence file: RDB08941_A5Kia.seq
>04388_08941_A5Ki_CSII-CMV5'_A05_02.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNGC TGAACGAGAA ANNGTAAAAT GATATAAATA 61 TCAATATATT AAATTAGATT TTGCATAAAA AACAGACTAC ATAATACTGT AAAACACAAC 121 ATATCCAGTC ACTATGGCGG CCGCATTAGG CACCCCAGGC TTTACACTTT ATGCTTCCGG 181 CTCGTATAAT GTGTGGATTT TGAGTTAGGA TCCGTCGAGA TTTTCAGGAG CTAAGGAAGC 241 TAAAATGGAG AAAAAAATCA CTGGATATAC CACCGTTGAT ATATCCCAAT GGCATCGTAA 301 AGAACATTTT GAGGCATTTC AGTCAGTTGC TCAATGTACC TATAACCAGA CCGTTCAGCT 361 GGATATTACG GCCTTTTTAA AGACCGTAAA GAAAAATAAG CACAAGTTTT ATCCGGCCTT 421 TATTCACATT CTTGCCCGCC TGATGAATGC TCATCCGGAA TTCCGTATGG CAATGAAAGA 481 CGGTGAGCTG GTGATATGGG ATAGTGTTCA CCCTTGTTAC ACCGTTTTCC ATGAGCAAAC 541 TGAAACGTTT TCATCGCTCT GGAGTGAATA CCACGACGAT TTCCGGCAGT TTCTACACAT 601 ATATTCGCAA GATGTGGCGT GTTACGGTGA AAACCTGGCC TATTTCCCTA AAGGGTTTAT 661 TGAGAATATG TTTTTCGTCT CAGCCAATCC CTGGGTGAGT TTCACCAGTT TTGATTTAAA 721 CGTGGCCAAT ATGGACAACT TCTTCGCCCC CGTTTTCACC ATGGGCAAAT ATTATACGCA 781 AGGCGACAAG GTGCTGATGC CGCTGGCGAT TCAGGTTCAT CATGCCGTTT GTGATGGCTT 841 CCATGTCNGC AGAATGCTTA ATGAATTACN ACAGTACTGC NATGANTGGC ANGGCGGGGC 901 GTAAACGCGN GNATNCNGCT NACTAAAAGC CAGATAACAG TATGCGNATT TGCNCNCTNA 961 NTTTTGCNGN ANNNNANNNT ATACNGNTAT GTATANCNNA NNNATGTNAA AAANNNNNNN 1021 NNN //
Primer: IRES-R Region: attR2,ccdB Sequence file: RDB08941_A5Kib.seq
>04388_08941_A5Ki_IRES-R_B05_05.ab1 1 NNNNNNNNNN NNNNNNNNNA NGTNAGGGGG GGGGGNNNNN NANGGGCGGN TCCGCGGCCG 61 CTGCAGAATT CTCGAGACCG GTAGCATCAC CACTTTGTAC AAGAAAGCTG AACGAGAAAC 121 GTAAAATGAT ATAAATATCA ATATATTAAA TTAGATTTTG CATAAAAAAC AGACTACATA 181 ATACTGTAAA ACACAACATA TCCAGTCACT ATGGTCGACC TGCAGACTGG CTGTGTATAA 241 GGGAGCCTGA CATTTATATT CCCCAGAACA TCAGGTTAAT GGCGTTTTTG ATGTCATTTT 301 CGCGGTGGCT GAGATCAGCC ACTTCTTCCC CGATAACGGA GACCGGCACA CTGGCCATAT 361 CGGTGGTCAT CATGCGCCAG CTTTCATCCC CGATATGCAC CACCGGGTAA AGTTCACGGG 421 AGACTTTATC TGACAGCAGA CGTGCACTGG CCAGGGGGAT CACCATCCGT CGCCCGGGCG 481 TGTCAATAAT ATCACTCTGT ACATCCACAA ACAGACGATA ACGGCTCTCT CTTTTATAGG 541 TGTAAACCTT AAACTGCATT TCACCAGCCC CTGTTCTCGT CAGCAAAAGA GCCGTTCATT 601 TCAATAAACC GGGCGACCTC AGCCATCCCT TCCTGATTTT CCGCTTTCCA GCGTTCGGCA 661 CGCAGACGAC GGGCTTCATT CTGCATGGTT GTGCTTACCN GACCGGANAN ATTGACATCA 721 TATATGCCTT GAGCNACTGA TAGCTGTCGC TGTCNACTGT CACTGTAATA CGCTGCTTCA 781 TANCATACCT CTTTTTGACA TACTTCGGNT ATACATATCA GTATATATTC NTATACNGCA 841 AAAATCAGCG CGCAAATACN CNTACTGNTA TCTGGNTTTT ANTAANCCNG ANNCNNNCNN 901 TTACGCCCCG NCCNGNNNNN CNTCNNANNA NTGNNGNNAN TNNNTNNNNN ATNCNGNNNN 961 NNTNNNNNNN ATCNNNAANN NNNNNNNGNN NNNNNNNN //
Primer: IRES-F Region: IRES2,Venus,PRE Sequence file: RDB08941_A5Kic.seq
>04388_08941_A5Ki_IRES-F_C05_08.ab1 1 NNNNNNNNNN NNNNNNNNNN TCNANGTTNA NNANNCGTCT AGGCCCCCCG AACCNCGGGG 61 ANGTGGTTTT CCTTTGAAAA ACACGATGAT AATATGGCCA CAACCATGGT GAGCAAGGGC 121 GAGGAGCTGT TCACCGGGGT GGTGCCCATC CTGGTCGAGC TGGACGGCGA CGTAAACGGC 181 CACAAGTTCA GCGTGTCCGG CGAGGGCGAG GGCGATGCCA CCTACGGCAA GCTGACCCTG 241 AAGCTGATCT GCACCACCGG CAAGCTGCCC GTGCCCTGGC CCACCCTCGT GACCACCCTG 301 GGCTACGGCC TGCAGTGCTT CGCCCGCTAC CCCGACCACA TGAAGCAGCA CGACTTCTTC 361 AAGTCCGCCA TGCCCGAAGG CTACGTCCAG GAGCGCACCA TCTTCTTCAA GGACGACGGC 421 AACTACAAGA CCCGCGCCGA GGTGAAGTTC GAGGGCGACA CCCTGGTGAA CCGCATCGAG 481 CTGAAGGGCA TCGACTTCAA GGAGGACGGC AACATCCTGG GGCACAAGCT GGAGTACAAC 541 TACAACAGCC ACAACGTCTA TATCACCGCC GACAAGCAGA AGAACGGCAT CAAGGCCAAC 601 TTCAAGATCC GCCACAACAT CGAGGACGGC GGCGTGCAGC TCGCCGACCA CTACCAGCAG 661 AACACCCCCA TCGGCGACGG CCCCGTGCTG CTGCCCGACA ACCACTACCT GAGCTACCAG 721 TCCGCCCTGA GCAAAGACCC CAACGAGAAG CGCGATCACA TGGTCCTGCT GGAGTTCGTG 781 ANCGCCGCCG GGATCACTCT CGGCATGGNC GANCTGTACN AGTAAGAATT CAAGGCCTCT 841 CGAGCCTCTA NAGTTAACAT CGAGGGNNCA AGCTTATCGA TAATCAACCT CTGGANTACA 901 AAATTTGTGA AAGANTGNNT GGTANNCTTA ACTATGTTGC TCCTTTTANN CTATGTGNAN 961 ACGCNGCTTT AANGNCTTTG TNTCANGNNA NTNGNTTCCC GNANGNNNTN CATTTTNNNN 1021 NNCNTNNNNN NNNNN //
Primer: HIVgp_R1 Sequence file: RDB08941_A3Dmd.seq
>04388_08941_A3Dm_HIVgp_R1_D11_11.ab1 1 CAGCTCTATT CCACTGCTCT TTTTTCTCTC TGCACCACTC TTCTCTTTGC CTTGGTGGGT 61 GCTACTCCTA ATGGTTCAAT TTTTACTACT TTATATTTAT ATAATTCACT TCTCCAATTG 121 TCCCTCATAT CTCCTCCTCC AGGTCTGAAG ATCAGCGGCC GGCCGCTTGC TGTGCGGTGG 181 TCTTACTTTT GTTTTGCTCT TCCTCTATCT TGTCTAAAGC TTCCTTGGTG TCTTTTATCT 241 CTATCCTTTG ATGCACACAA TAGAGGGTTG CTACTGTATT ATATAATGAT CTAAGTTCTT 301 CTGATCCTGT CTGAAGGGAT GGTTGTAGCT GTCCCAGTAT TTGTCTACAG CCTTCTGATG 361 TTTCTAACAG GCCAGGATTA ACTGCGAATC GTTCTAGCTC CCTGCTTGCC CATACTATAT 421 GTTTTAATTT ATATTTTTTC TTTCCCCCTG GCCTTAACCG AATTTTTTCC CATCGCGATC 481 TAATTCTCCC CCGCTTAATA CTGACGCTCT CGCACCCATC TCTCTCCTTC TAGCCTCCGC 541 TAGTCAAAAT TTTTGGCGTA CTCACCAGTC GCCGCCCCTC GCCTCTTGCC GTGCGCGCTT 601 CAGCAAGCCG AGTCCTGCGT CGAGAGAGCT CCTCTGGTTT CCCTTTCGCT TTCAAGTCCC 661 TGTTCGGGCG CCACTGCTAG AGATTTTCCA CACTGACTAA AAGGGTCTGA GGGATCTCTA 721 GTTACCAGAG TCACACAACA GACGGGCACA CACTACTTGA AGCACTCAAG GCAAGCTTTA 781 TTGAGGCTTA AGCAGTGGGT TCCCTAGTTA GCCAGAGAGC TCCCAGGCTC AGATCTGGTC 841 TAACCAGAGA GACCCAGTAC AGGCAAAACG CGCTGCTTAT ATAGACCTCC CACCGTACAC 901 GCCTACCGCC CATTTGCGTC AATGGGGGCG GAGTTGTTAC GACATTTTGG AAAGTCCCGT 961 TGATTTTGGT GCCAAAACAA ACTCCCATTG ACGTCAATGG GGGTGGAGAC TTGGAAATCC 1021 CCGTGAGTCA AACCGCTATC CACGCCCATT GATGTACTGC CCAAAACCGC ATCACCATGG 1081 TAATAGCGAT GACTAAATAC GTAGATGTAC TGGCTAGGTT AGAAAGGTCC CATATGTTCA 1141 TGTACTGGGG CATAATCCCA AGGGCCGGGG CCCAATA //
Primer: BGH_rev Sequence file: RDB08941_A3Dme.seq
>04388_08941_A3Dm_BGHrev_E11_14.ab1 1 GGTACGGGCT GCTAGAGATT TTCCACACTG ACTAAAAGGG TCTGAGGGAT CTCTAGTTAC 61 CAGAGTCACA CAACAGACGG GCACACACTA CTTGAAGCAC TCAAGGCAAG CTTTATTGAG 121 GCTTAAGCAG TGGGTTCCCT AGTTAGCCAG AGAGCTCCCA GGCTCAGATC TGGTCTAACC 181 AGAGAGACCC AGTACAGTCC GGATGCAGCT CTCGGGCCAT GTGATGAAAT GCTAGGCGGC 241 TGTCAAACCT CCACTCTAAT ACTTCTCTCT CCGGGTCATC CATCCCATGC AGGCTCACAG 301 GGTGTAACAA GCGGGTGTTC TCTCCTTCAT TGGCTTCTTC TACCTTCTCT TGCTCAACTG 361 GTACTAGCTT GTAGCACCAT CCAAAGGTCA GTGGATATCT GATCCCTGGC CCTGGTGTGT 421 AGTTCTGCCA ATCAGGGAAG TAGCCTTGTG TGTGGTAGAT CCACAGATCA AGGATATCTT 481 GTCTTCGTTG GGAGTGAATT AGCCCTTCCA GTCCCCCCTT TTCTTTTAAA AAGTGGCTAA 541 GATCTACAGC TGCCTTGTAA GTCATTGGTC TTAAAGGTAC CTGAGGTGTG ACTGGAAAAC 601 CCACCTCCTC CTCCTCTTGT GCTTCTAGCC AGGCACAATC AGCATTGGTA GCTGCTGTAT 661 TGCTACTTGT GATTGCTCCA TGTTTTTCTA GGTCTCGACG GTATCGATGC GGGGAGGCGG 721 CCCAAAGGGA GATCCGACTC GTCTGAGGGC GAAGGCGAAG ACGCGGAAGA GGCCGCAGAG 781 CCGGCAGCAG GCCGCGGGAA GGAAGGTCCG CTGGATTGAG GGCCGAAGGG ACGTAGCAGA 841 AGGACGTCCC GCGCAGAATC CAGGTGGCAA CACAGGCGAG CAGCCATGGA AAGGACGTCA 901 GCTTCCCCGA CAACACCACG GAATTGTCAG TGCCCAACAG CCGAGCCCCT GTCCAGCAGC 961 GGGCAAAGGC AGGCGGCGAT GAGTTCCGCC GTGGCAATAG GGAGGGGGAA AGCGAAAGTC 1021 CCGGGAAAGG AGCTGACAGG TGGTGGCAAT GCCCCAACCA GTGGGGGGTT TGCGTCAGCA 1081 AACACAGTGC ACACCACGCC ACGTTTGCCT GACACGACAC ACTCCTCATT AAAGAGACAG 1141 CACCAAGGAT TTTTATTACC AACGGAAGGG AGAAAAAATT GA //
Primer: SV40pro_F Sequence file: RDB08941_A3Dmf.seq
>04388_08941_A3Dm_SV40pro_F_F11_17.ab1 1 GCCTGTCCTG GACATTCAGA GTAGTGAGGA GGCTTTTTTG GAGGCCTAGG CTTTTGCAAA 61 AAGCTCCCGG GAGCTTGTAT ATCCATTTTC GGATCTGATC AGCACGTGTT GACAATTAAT 121 CATCGGCATA GTATATCGGC ATAGTATAAT ACGACAAGGT GAGGAACTAA ACCATGGCCA 181 AGTTGACCAG TGCCGTTCCG GTGCTCACCG CGCGCGACGT CGCCGGAGCG GTCGAGTTCT 241 GGACCGACCG GCTCGGGTTC TCCCGGGACT TCGTGGAGGA CGACTTCGCC GGTGTGGTCC 301 GGGACGACGT GACCCTGTTC ATCAGCGCGG TCCAGGACCA GGTGGTGCCG GACAACACCC 361 TGGCCTGGGT GTGGGTGCGC GGCCTGGACG AGCTGTACGC CGAGTGGTCG GAGGTCGTGT 421 CCACGAACTT CCGGGACGCC TCCGGGCCGG CCATGACCGA GATCGGCGAG CAGCCGTGGG 481 GGCGGGAGTT CGCCCTGCGC GACCCGGCCG GCAACTGCGT GCACTTCGTG GCCGAGGAGC 541 AGGACTGACA CGTGCTACGA GATTTCGATT CCACCGCCGC CTTCTATGAA AGGTTGGGCT 601 TCGGAATCGT TTTCCGGGAC GCCGGCTGGA TGATCCTCCA GCGCGGGGAT CTCATGCTGG 661 AGTTCTTCGC CCACCCCAAC TTGTTTATTG CAGCTTATAA TGGTTACAAA TAAAGCAATA 721 GCATCACAAA TTTCACAAAT AAAGCATTTT TTTCACTGCA TTCTAGTTGT GGTTTGTCCA 781 AACTCATCAA TGTATCTTAT CATGTCTGTA TACCGTCGAC CTCTAGCTAG AGCTTGGCGT 841 AATCATGGTC ATAGCTGTTT CCTGTGTGAA ATTGTTATCC GCTCACAATT CCACACAACA 901 TACGAGCCGG AAGCATAAAG TGTAAAGCCT GGGGTGCCTA ATGAGTGAGC TAACTCACAT 961 TAATTGCGTT GCGCTCACTG CCCGCTTTCC AGTCGGGAAA CCCTGTCGTG CCAGCTGCAT 1021 TAATGAATCG GGCCAACGCG CGGGGAGAGG CGGTTTGCGT ATTGGGGCGC TCTTCGCTTT 1081 CTCGCCTCAC TGACTCGCTG CCGCTCCGCT CGATCCGGCT GCCGCGAAGC GTAATCCAGC 1141 TCACTCAAGC GTATACGGTT AATTCCACCA G //
Primer: SV40pro_ori_F Region: SV40 pro_ori,Zeo Sequence file: RDB08941_A5Kig.seq
>04388_08941_B0G9S_SV40pro_ori_F_E10_13_ABI24.ab1 1 NNNNNNNNNN NNNNNGNCGC CTCTGCCTCT GNNCTATTCN NGAAGTAGTG AGGANGCTTT 61 TTTGGAGNCC TAGGCTTTTG CAAAAAGCTC CCGGGAGCTT GTATATCCAT TTTCGGATCT 121 GATCAGCACG TGTTGACAAT TAATCATCGG CATAGTATAT CGGCATAGTA TAATACGACA 181 AGGTGAGGAA CTAAACCATG GCCAAGTTGA CCAGTGCCGT TCCGGTGCTC ACCGCGCGCG 241 ACGTCGCCGG AGCGGTCGAG TTCTGGACCG ACCGGCTCGG GTTCTCCCGG GACTTCGTGG 301 AGGACGACTT CGCCGGTGTG GTCCGGGACG ACGTGACCCT GTTCATCAGC GCGGTCCAGG 361 ACCAGGTGGT GCCGGACAAC ACCCTGGCCT GGGTGTGGGT GCGCGGCCTG GACGAGCTGT 421 ACGCCGAGTG GTCGGAGGTC GTGTCCACGA ACTTCCGGGA CGCCTCCGGG CCGGCCATGA 481 CCGAGATCGG CGAGCAGCCG TGGGGGCGGG AGTTCGCCCT GCGCGACCCG GCCGGCAACT 541 GCGTGCACTT CGTGGCCGAN GANCAGGACT GACACGTGCT ACGAGATTTC GATTCCACCG 601 CCGCCTTCTA TGAAAGGTTG GGCTTCGGAA TCGTTTTCCG GGACGCCGGC TGGATGATCC 661 TCCAGCGCGG GGNTCTCATG CTGGAGTTCT TCGCCCACCC CAACTTGTTT ATTGCAGCTT 721 ATAATGGTTA CAAATAAAGC AATAGCATCA CAAATTTCAC AAATAAAGCA TTTTTTTTCA 781 CTGCATTCTA GTTGTGGNTN GNCNNAACTC ATCAATGTAT CTTATCATGT CTGTATACNN 841 NCNANNNCNA NCNANNNCTT GNCNNNATCN NNGNNCATAN CNGTTTCCNN NNNNAAANNN 901 NNNTNCGNNN ANNNNNNCNN NNNNNNNN //