CSII-EF-RfA (#RDB04387)
Backbone vector plasmid of lentivirus construct by Gateway(R) cloning with EF-1 alpha promoter.
 Drawn by SnapGene® software |
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| Clone info. | Backbone vector plasmid of lentivirus construct by Gateway(R) cloning with EF-1 alpha promoter. |
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| Comment | pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394) are popular choices for lentivirus packaging. |
| Vector backbone | Plasmid |
| Selectable markers | Ampicillin, Chloramphenicol, ccdB (E. coli). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector. |
| Growth conditions | 37C, LB+Amp |
| Growth remarks | Use DB3.1, ccdB Survival or equivalent to amplify this clone. 30 degC is fine. |
| Depositor|Developer | Miyoshi, Hiroyuki | |
Distribution information
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
| Ordering forms | Order form [Credit Card Payment  ] [Bank Transfer Payment] [Example of order form ]MTA, for use for not-for-profit academic purpose [Word ] [Example of MTA ]Please visit Ordering instruction.[link] |
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| Terms and conditions for distribution | The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer. |
| Remarks | Remember that you will be working with samples containing infectious virus. Use Survival2 (Invitrogen) or equivalent to amplify this clone. |
[open/close]| 必要書類 | 提供依頼書 [依頼書の記入例 ]提供同意書 (MTA, 非営利学術目的用)[Word ] [MTAの記入例 ]手続きの概要は、「レンチウイルスベクターの提供申し込み」をご覧ください。 |
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| MTAに書く使用条件 | 本件研究材料は、非営利機関の非営利学術研究に限って提供する。本件研究材料を利用した研究結果等を発表する際は、本件研究材料が故三好浩之博士により開発されたことを明示する。 |
| 備考 | このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。 このクローンの増殖にはSurvival2 (Invitrogen)あるいは同等の宿主を使用してください。 |
| Catalog # | Resource name | Shipping form | Fee |
|---|---|---|---|
| RDB04387 | CSII-EF-RfA | DNA solution |
How to cite this biological resource
Materials & Methods section:
| The CSII-EF-RfA was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB04387). |
Reference section:
Further references such as user reports and related articles (go to bottom)
References
Original, user report and related articles
| user_report | Yamamoto, S., Bovine respiratory syncytial virus enhances the attachment of Trueperella pyogenes to cells. J. Vet. Med. Sci. 86 (10): 1068-1075 (2024). PMID 39111845. [PubMed] [Article] [RRC of NBRP] |
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| user_report | Kawamura, Y., Cellular senescence induction leads to progressive cell death via the INK4a-RB pathway in naked mole-rats. EMBO J. 2023 Aug 15;42(16):e111133 PMID 37431790. [PubMed] [Article] [RRC of NBRP] |
| user_report | Nishitani-Isa, M., Trapping of CDC42 C-terminal variants in the Golgi drives pyrin inflammasome hyperactivation. J. Exp. Med. 219 (6): e20211889 (2022). PMID 35482294. [PubMed] [Article] [RRC of NBRP] |
| user_report | Takahashi, K., TGF-β generates a population of cancer cells residing in G1 phase with high motility and metastatic potential via KRTAP2-3. Cell Rep. 40 (13): 111411 (2022). PMID 36170816. [PubMed] [Article] [RRC of NBRP] |
| user_report | Ozawa, T., Systemic administration of monovalent follistatin-like 3-Fc-fusion protein increases muscle mass in mice. iScience 24 (5): 102488 (2021). PMID 34113826. [PubMed] [Article] [RRC of NBRP] |
| user_report | Ozawa, T., Preparation of monovalent follistatin-like 3-Fc-fusion protein and evaluation of its effects on muscle mass in mice. STAR Protoc. 2 (4): 100839 (2021). PMID 34585166. [PubMed] [Article] [RRC of NBRP] |
| user_report | Nishida, J., Epigenetic remodelling shapes inflammatory renal cancer and neutrophil-dependent metastasis. Nat. Cell Biol. 22 (4): 465-475 (2020). PMID 32203421. [PubMed] [Article] [RRC of NBRP] |
| user_report | Seki, R., Induced pluripotent stem cell-derived monocytic cell lines from a NOMID patient serve as a screening platform for modulating NLRP3 inflammasome activity. PLoS One 15 (8): e0237030 (2020). PMID 32810141. [PubMed] [Article] [RRC of NBRP] |
| user_report | Morikawa, M., The ALK-1/SMAD/ATOH8 axis attenuates hypoxic responses and protects against the development of pulmonary arterial hypertension. Sci. Signal. 12(607). pii: eaay4430 (2019). PMID 31719172. [PubMed] [Article] [RRC of NBRP] |
| user_report | Kambara, H., Gasdermin D Exerts Anti-inflammatory Effects by Promoting Neutrophil Death. Cell Rep. 22 (11): 2924-2936 (2018). PMID 29539421. [PubMed] [Article] [RRC of NBRP] |
| user_report | Kubota, S.I., Whole-Body Profiling of Cancer Metastasis with Single-Cell Resolution. Cell Rep. 20 (1): 236-250 (2017). PMID 28683317. [PubMed] [Article] [RRC of NBRP] |
| user_report | Yamamoto, E., Establishment and characterization of cell lines derived from complete hydatidiform mole. Int. J. Mol. Med. 40 (3): 614-622 (2017). PMID 28713902. [PubMed] [Article] [RRC of NBRP] |
| user_report | Suzuki, T., RUNX1 regulates site specificity of DNA demethylation by recruitment of DNA demethylation machineries in hematopoietic cells. Blood Adv. 1 (20): 1699-1711 (2017). PMID 29296817. [PubMed] [Article] [RRC of NBRP] |
| user_report | Imamura, Y., Generation of Large Numbers of Antigen-Expressing Human Dendritic Cells Using CD14-ML Technology. PLoS One. 2016 Apr 6;11(4):e0152384 (2016). PMID 27050553. [PubMed] [Article] [RRC of NBRP] |
| user_report | Inagawa, Y., A human cancer xenograft model utilizing normal pancreatic duct epithelial cells conditionally transformed with defined oncogenes. Carcinogenesis 35 (8): 1840-1846 (2014). PMID 24858378. [PubMed] [Article] [RRC of NBRP] |
| user_report | Moriyama, H., Tightly regulated and homogeneous transgene expression in human adipose-derived mesenchymal stem cells by lentivirus with tet-off system. PLoS One, 8 (6): e66274 (2013). PMID 23776652. [PubMed] [Article] [RRC of NBRP] |
| user_report | Yugawa, T., Noncanonical NOTCH signaling limits self-renewal of human epithelial and induced pluripotent stem cells through ROCK activation. Mol. Cell. Biol., 233 (22): 4434-4447 (2013). PMID 24019071. [PubMed] [Article] [RRC of NBRP] |
| user_report | Yoshita, M., Activation of mTOR by human T-cell leukemia virus type 1 Tax is important for the transformation of mouse T cells to interleukin-2-independent growth. Cancer Sci., 103 (2): 369-374 (2012). PMID 22010857. [PubMed] [Article] [RRC of NBRP] |
| user_report | Suzuki, T.,, Reconstruction of monocyte transcriptional regulatory network accompanies monocytic functions in human fibroblasts. PLoS One, 7 (3): e33474 (2012). PMID 22428058. [PubMed] [Article] [RRC of NBRP] |
| user_report | Makino, K., Discoidin domain receptor 2-microRNA 196a-mediated negative feedback against excess type I collagen expression is impaired in scleroderma dermal fibroblasts. J. Invest. Dermatol., 133 (1): 110-119 (2012). PMID 22832484. [PubMed] [Article] [RRC of NBRP] |
| user_report | Moriyama, M., Human adipose tissue-derived multilineage progenitor cells exposed to oxidative stress induce neurite outgrowth in PC12 cells through p38 MAPK signaling. BMC Cell Biol. 2012 Aug 7;13:21 (2012). PMID 22870983. [PubMed] [Article] [RRC of NBRP] |
| user_report | Kataoka, A., Involvement of vasodilator-stimulated phosphoprotein in UDP-induced microglial actin aggregation via PKC- and Rho-dependent pathways. Purinerg. Signal, 7 (4): 403-411 (2011). PMID 21567128. [PubMed] [Article] [RRC of NBRP] |
| user_report | Yokomine K., The forkhead box M1 transcription factor as a candidate of target for anti-cancer immunotherapy. Int. J. Cancer., 126, 2153-2163 (2010). PMID 19688828. [PubMed] [Article] [RRC of NBRP] |
| user_report | Ito S., Expression of a chemokine BRAK/CXCL14 in oral floor carcinoma cells reduces the settlement rate of the cells and suppresses their proliferation in vivo. Biomed. Res., 31, 199-206 (2010). PMID 20622470. [PubMed] [Article] [RRC of NBRP] |
| user_report | Harao M., HLA-A2-restricted CTL epitopes of a novel lung cancer-associated cancer testis antigen, cell division cycle associated 1, can induce tumor-reactive CTL. Int. J. Cancer., 123, 2616-2625 (2008). PMID 18770861. [PubMed] [Article] [RRC of NBRP] |
| user_report | Imai K., Identification of a novel tumor-associated antigen, cadherin 3/P-cadherin, as a possible target for immunotherapy of pancreatic, gastric, and colorectal cancers. Clin. Cancer. Res., 14, 6487-6495 (2008). PMID 18927288. [PubMed] [Article] [RRC of NBRP] |
| user_report | Irie A., Protein kinase D2 contributes to either IL-2 promoter regulation or induction of cell death upon TCR stimulation depending on its activity in Jurkat cells. Int. Immunol., 18, 1737-1747 (2006). PMID 17077180. [PubMed] [Article] [RRC of NBRP] |
| user_report | Tanabe, R., PRRX1 induced by BMP signaling decreases tumorigenesis by epigenetically regulating glioma-initiating cell properties via DNA methyltransferase 3A. Mol. Oncol. 16 (1): 269-288 (2022). PMID 34214250. [PubMed] [Article] [RRC of NBRP] |
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