RDB04387 - CSII-EF-RfA

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CSII-EF-RfA

Backbone vector plasmid of lentivirus construct by Gateway(R) cloning with EF-1 alpha promoter.

Catalog number	RDB04387
Resource name	CSII-EF-RfA
Clone info.	Backbone vector plasmid of lentivirus construct by Gateway(R) cloning with EF-1 alpha promoter. Commonly requested with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Vector backbone	CSII-EF-RfA (Plasmid)
Selectable markers	Please note that Zeo resistance marker is not included in the lentivirus produced from this vector. E. coli: Amp^r, Cm^r, ccdB.
Growth remarks	Use DB3.1, ccdB Survival or equivalent to amplify this clone. 30 degC is fine.
Depositor\|Developer	Miyoshi, Hiroyuki \|

Lentiviral plasmid list, maps and sequences information. [link]

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer.

Ordering Please visit Ordering instruction.[link]
Order form [Credit Card Payment] [Bank Transfer or Check Payment]
Material Transfer Agreement (MTA, for use for not-for-profit academic purpose)[Word]

Remarks Remember that you will be working with samples containing infectious virus.
Use Survival2 (Invitrogen) or equivalent to amplify this clone.

Distribution information (domestic users) [open/close]

Terms and conditions set forth by the DEPOSITOR	The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer.
Ordering	Please visit Ordering instruction.[link] Order form [Credit Card Payment] [Bank Transfer or Check Payment] Material Transfer Agreement (MTA, for use for not-for-profit academic purpose)[Word]
Remarks	Remember that you will be working with samples containing infectious virus. Use Survival2 (Invitrogen) or equivalent to amplify this clone.

提供条件	本件研究材料は、非営利機関の非営利学術研究に限って提供する。本件研究材料を利用した研究結果等を発表する際は、本件研究材料が故三好浩之博士により開発されたことを明示する。
提供依頼	手続きの詳細は、「レンチウイルスベクターの提供申し込み」をご覧ください。提供依頼書 [Word] 提供同意書 (非営利機関による非営利学術研究用)[Word] 遺伝子組換え生物の受入れ確認書 [Word]
備考	【追加提供依頼書】受入れ確認書(書式J) このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。このクローンの増殖にはSurvival2 (Invitrogen)あるいは同等の宿主を使用してください。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB04387	CSII-EF-RfA	DNA solution

Ordering instruction of plasmids [in Japanese] [in English]
Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

References and tips

Electronic file

Electronic file	Sequence RDB04387z.seq
Electronic file	Remarks, protocol and/or map (pdf) RDB04387.pdf

Original reference

Further references such as user reports and related articles (go to bottom)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB12131_A2H6p1-3.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: EF1a-C_F Region: EF-1alpha pro,RfA(attR1,lacUV5 pro,CmR) Sequence file: RDB12131_A2H6e.seq
>04387_04387_A2H6_EF1a-C_F_C05_08_ABI24.ab1 1 NNNNNNNNNN NNNNTTTTGA NTTTGGNNCT TGGTTNNTTC TCNAGCCTCA GACAGTGGTT 61 CAAAGTTTTT TTCTTCCATT TCAGGTGTCG TGAACACGCT ACCGGTCTCG AGAATTATCA 121 CAAGTTTGTA CAAAAAAGCT GAACGAGAAA CGTAAAATGA TATAAATATC AATATATTAA 181 ATTAGATTTT GCATAAAAAA CAGACTACAT AATACTGTAA AACACAACAT ATCCAGTCAC 241 TATGGCGGCC GCATTAGGCA CCCCAGGCTT TACACTTTAT GCTTCCGGCT CGTATAATGT 301 GTGGATTTTG AGTTAGGATC CGTCGAGATT TTCAGGAGCT AAGGAAGCTA AAATGGAGAA 361 AAAAATCACT GGATATACCA CCGTTGATAT ATCCCAATGG CATCGTAAAG AACATTTTGA 421 GGCATTTCAG TCAGTTGCTC AATGTACCTA TAACCAGACC GTTCAGCTGG ATATTACGGC 481 CTTTTTAAAG ACCGTAAAGA AAAATAAGCA CAAGTTTTAT CCGGCCTTTA TTCACATTCT 541 TGCCCGCCTG ATGAATGCTC ATCCGGAATT CCGTATGGCA ATGAAAGACG GTGAGCTGGT 601 GATATGGGAT AGTGTTCACC CTTGTTACAC CGTTTTCCAT GAGCAAACTG AAACGTTTTC 661 ATCGCTCTGG AGTGAATACC ACGACGATTT CCGGCAGTTT CTACACATAT ATTCGCAAGA 721 TGTGGCGTGT TACGGTGAAA ACCTGNCCTA TTTCCCTAAA GGGTTTATTG AGAATATGTT 781 TTTCGTCTCA GCCAATCCCT GGGTGAGTTT CACCAGTTTT GATTTAAACG TGGCCNATAT 841 GGACAACTTC TTCGCCCCCG TTTTCACCAN GGGCNAATAT TATACGCNAG GCGNNNANGT 901 GCTGANGNCG CTGGCNANTC NNNNCATCAT GCCNTTNGNG ATGGNNTCCA NGTCGGCNNA 961 ATGCTTNNTG NNNTACNANN NNANNGCNNT GANNNNNNNN NN //
Primer: ccdB-C_F Region: RfA(ccdB,attR2),PRE Sequence file: RDB12131_A2H6f.seq
>04387_04387_A2H6_ccdB-C_F_D05_11_ABI24.ab1 1 NNNNNNNNNN NNNNNTCGGG GNNGAANCTG GCGCATGATG ACCNCCGATA TGGCCAGTGT 61 GCCGGTCTCC GTTATCGGGG AAGAAGTGGC TGATCTCAGC CACCGCGAAA ATGACATCAA 121 AAACGCCATT AACCTGATGT TCTGGGGAAT ATAAATGTCA GGCTCCCTTA TACACAGCCA 181 GTCTGCAGGT CGACCATAGT GACTGGATAT GTTGTGTTTT ACAGTATTAT GTAGTCTGTT 241 TTTTATGCAA AATCTAATTT AATATATTGA TATTTATATC ATTTTACGTT TCTCGTTCAG 301 CTTTCTTGTA CAAAGTGGTG ATAATTCTGC AGCGGCCGCG GATCCTCTAG AGTTAACATC 361 GAGGGATCAA GCTTATCGAT AATCAACCTC TGGATTACAA AATTTGTGAA AGATTGACTG 421 GTATTCTTAA CTATGTTGCT CCTTTTACGC TATGTGGATA CGCTGCTTTA ATGCCTTTGT 481 ATCATGCTAT TGCTTCCCGT ATGGCTTTCA TTTTCTCCTC CTTGTATAAA TCCTGGTTGC 541 TGTCTCTTTA TGAGGAGTTG TGGCCCGTTG TCAGGCAACG TGGCGTGGTG TGCACTGTGT 601 TTGCTGACGC AACCCCCACT GGTTGGGGCA TTGCCACCAC CTGTCAGCTC CTTTCCGGGA 661 CTTTCGCTTT CCCCCTCCCT ATTGCCACGG CGGAACTCAT CGCCGCCTGC CTTGCCCGCT 721 GCTGGACAGG GGCTCGGCTG TTGGGCACTG ACAATTCCGT GGTGTTGTCG GGGAANCTGA 781 CGTCCTTTCC NTGGCTGCTC GCCTGTGTTG CCACCTGGAT TCTGCGCGGG ANGTCCTTCT 841 GCTACGTCCC TTCGGCCNTC NATCCAGCGG ANCTTNCTTN CCGCGGCCTG CTGNNGGNNN 901 TGCGGNNNCT TNNNNGNCTT CNCCTTCGCC CTCAGACGAG TCGGATCNNC NNTTGGGCCN 961 NCNCCCCGCN TCNATANNNN CNNNANNNAN AAAAANNTNN //
Primer: CMV_Forward Region: CMVpro,5'LTR,HIV-1psi Sequence file: RDB12131_A2H6g.seq
>04387_04387_A2H6_CMV_Forward_E05_14_ABI24.ab1 1 NNNNNNNNNN NNNNNTGNNG CNNNGGGCGG TAGGCGTGTA CGGTGGGAGG TCTATATAAG 61 CAGCGCGTTT TGCCTGTACT GGGTCTCTCT GGTTAGACCA GATCTGAGCC TGGGAGCTCT 121 CTGGCTAACT AGGGAACCCA CTGCTTAAGC CTCAATAAAG CTTGCCTTGA GTGCTTCAAG 181 TAGTGTGTGC CCGTCTGTTG TGTGACTCTG GTAACTAGAG ATCCCTCAGA CCCTTTTAGT 241 CAGTGTGGAA AATCTCTAGC AGTGGCGCCC GAACAGGGAC TTGAAAGCGA AAGGGAAACC 301 AGAGGAGCTC TCTCGACGCA GGACTCGGCT TGCTGAAGCG CGCACGGCAA GAGGCGAGGG 361 GCGGCGACTG GTGAGTACGC CAAAAATTTT GACTAGCGGA GGCTAGAAGG AGAGAGATGG 421 GTGCGAGAGC GTCAGTATTA AGCGGGGGAG AATTAGATCG CGATGGGAAA AAATTCGGTT 481 AAGGCCAGGG GGAAAGAAAA AATATAAATT AAAACATATA GTATGGGCAA GCAGGGAGCT 541 AGAACGATTC GCAGTTAATC CTGGCCTGTT AGAAACATCA GAAGGCTGTA GACAAATACT 601 GGGACAGCTA CAACCATCCC TTCAGACAGG ATCAGAAGAA CTTAGATCAT TATATAATAC 661 AGTAGCAACC CTCTATTGTG TGCATCAAAG GATAGAGATA AAAGACACCA AGGAAGCTTT 721 AGACAAGATA GAGGAAGAGC AAAACAAAAG TAAGANCACC GCACAGCAAG GGCCGCTGAT 781 CTTCAGACNT GGAGGANGNN NTATGANGGA NAATTGGAGA AGTGAATTAT ATAAATATAA 841 AGTAGTAAAA ATTGAACCNT TANGAGTAGC ACNNNCNNAN GNNNNGAGAA NANNGGTGCA 901 GANAGAAAAA AAGANCAGNG GGAATAGGAN NTTTGTNNCT NNGGGNNCNN GGNNNCAGCN 961 NNAANCNCTA NNGGNNNNNN NNNN //
Primer: cPPT/CTS-E_R Region: cPPT_CTS,RRE Sequence file: RDB12131_A2H6h.seq
>04387_04387_A2H6_cPPT_CTS-E_R_F05_17_ABI24.ab1 1 NNNNNNNNNT NGNTTTTNNN NNCTTTAGTT TGTATGTCTG TTGCTATTAT GTCTACTATT 61 CTTTCCCCTG CACTGTACCC CCCAATCCCC CCTTTTCTTT TAAAATTGTG GATGAATACT 121 GCCATTTGTA GATCCGTTCA CTAATCGAAT GGATCTGTCT CTGTCTCTCT CTCCACCTTC 181 TTCTTCTATT CCTTCGGGCC TGTCGGGTCC CCTCGGGGTT GGGAGGTGGG TCTGAAACGA 241 TAATGGTGAA TATCCCTGCC TAACTCTATT CACTATAGAA AGTACAGCAA AAACTATTCT 301 TAAACCTACC AAGCCTCCTA CTATCATTAT GAATAATTTT ATATACCACA GCCAATTTGT 361 TATGTTAAAC CAATTCCACA AACTTGCCCA TTTATCTAAT TCCAATAATT CTTGTTCATT 421 CTTTTCTTGC TGGTTTTGCG ATTCTTCAAT TAAGGAGTGT ATTAAGCTTG TGTAATTGTT 481 AATTTCTCTG TCCCACTCCA TCCAGGTCGT GTGATTCCAA ATCTGTTCCA GAGATTTATT 541 ACTCCAACTA GCATTCCAAG GCACAGCAGT GGTGCAAATG AGTTTTCCAG AGCAACCCCA 601 AATCCCCAGG AGCTGTTGAT CCTTTAGGTA TCTTTCCACA GCCAGGATTC TTGCCTGGAG 661 CTGCTTGATG CCCCAGACTG TGAGTTGCAA CAGATGCTGT TGCGCCTCAA TAGCCCTCAG 721 CAAATTGTTC TGCTGCTGCA CTATACCAGA CAATAATTGT CTGGCCTGTA NCGTCAGCGT 781 CATTGACGCT GCGCNCATAG TGCTTCCTGC TGCTCNNNAG AACCCAAGGA ACAAAGCTCC 841 TATTCCCACT GCTCTTTTTT TCTCTCTGCA CCACTCTTCT CTTTGCCTNG GNGGGTGCTA 901 CTCCNAANGG TTCAATTTTN NCTACTTTAT ATTTATATAN NTCACTTCTN NNATTGTCCN 961 NCATANCNNC NNNNNCANNN NNNNNNN //
Primer: bGH-B_R Region: 3'LTR,PRE Sequence file: RDB12131_A2H6i.seq
>04387_04387_A2H6_bGH-B_R_G05_20_ABI24.ab1 1 NNNNNNNNNN NNNNTNNNNN GCGGGTNNNN NNGGCCCTGC TAGAGANTTT CCNCACTGAC 61 TAAAAGGGTC TGAGGGATCT CTAGTTACCA GAGTCACACA ACAGACGGGC ACACACTACT 121 TGAAGCACTC AAGGCAAGCT TTATTGAGGC TTAAGCAGTG GGTTCCCTAG TTAGCCAGAG 181 AGCTCCCAGG CTCAGATCTG GTCTAACCAG AGAGACCCAG TACAGTCCGG ATGCAGCTCT 241 CGGGCCATGT GATGAAATGC TAGGCGGCTG TCAAACCTCC ACTCTAATAC TTCTCTCTCC 301 GGGTCATCCA TCCCATGCAG GCTCACAGGG TGTAACAAGC GGGTGTTCTC TCCTTCATTG 361 GCTTCTTCTA CCTTCTCTTG CTCAACTGGT ACTAGCTTGT AGCACCATCC AAAGGTCAGT 421 GGATATCTGA TCCCTGGCCC TGGTGTGTAG TTCTGCCAAT CAGGGAAGTA GCCTTGTGTG 481 TGGTAGATCC ACAGATCAAG GATATCTTGT CTTCGTTGGG AGTGAATTAG CCCTTCCAGT 541 CCCCCCTTTT CTTTTAAAAA GTGGCTAAGA TCTACAGCTG CCTTGTAAGT CATTGGTCTT 601 AAAGGTACCT GAGGTGTGAC TGGAAAACCC ACCTCCTCCT CCTCTTGTGC TTCTAGCCAG 661 GCACAATCAG CATTGGTAGC TGCTGTATTG CTACTTGTGA TTGCTCCATG TTTTTCTAGG 721 TCTCGACGGT ATCGATGCGG GGAGGCGGCC CAAAGGGAGA TCCGACTCGT CTGANGGCGA 781 ANGCGAAGAN GCGGAANANG CCGCAGANCC GGCAGCANGC CGCGNNAANG AANGTCCGCT 841 GGATTGAGGG CCGAANGGAC GTAGCAGAAN GANGTCCCGC GCAGAATCCA GGTGGCAACA 901 CANGCNANCA GCCNTGGAAA GGNNGTCAGC TTCCCCNNNN ACACCNCNNN NNTGNCAGNG 961 CCNNACAGNC NANCCNNNNN NNNNCANCGG NNNANNNNNN NNNN //
Primer: SV40pro_ori_F Region: SV40pro_ori,Zeo,SV40p(A) Sequence file: RDB12131_A2H6j.seq
>04387_04387_A2H6_SV40pro_ori_F_H05_23_ABI24.ab1 1 NNNNNNNNNN NNNNNNNANG NCGCCTCTGC CTCTGANCTA TTCNNGAAGT AGTGAGGAGG 61 CTTTTTTGGA GGCCTAGGCT TTTGCAAAAA GCTCCCGGGA GCTTGTATAT CCATTTTCGG 121 ATCTGATCAG CACGTGTTGA CAATTAATCA TCGGCATAGT ATATCGGCAT AGTATAATAC 181 GACAAGGTGA GGAACTAAAC CATGGCCAAG TTGACCAGTG CCGTTCCGGT GCTCACCGCG 241 CGCGACGTCG CCGGAGCGGT CGAGTTCTGG ACCGACCGGC TCGGGTTCTC CCGGGACTTC 301 GTGGAGGACG ACTTCGCCGG TGTGGTCCGG GACGACGTGA CCCTGTTCAT CAGCGCGGTC 361 CAGGACCAGG TGGTGCCGGA CAACACCCTG GCCTGGGTGT GGGTGCGCGG CCTGGACGAG 421 CTGTACGCCG AGTGGTCGGA GGTCGTGTCC ACGAACTTCC GGGACGCCTC CGGGCCGGCC 481 ATGACCGAGA TCGGCGAGCA GCCGTGGGGG CGGGAGTTCG CCCTGCGCGA CCCGGCCGGC 541 AACTGCGTGC ACTTCGTGGC CGAGGAGCAG GACTGACACG TGCTACGAGA TTTCGATTCC 601 ACCGCCGCCT TCTATGAAAG GTTGGGCTTC GGAATCGTTT TCCGGGACGC CGGCTGGATG 661 ATCCTCCAGC GCGGGGATCT CATGCTGGAG TTCTTCGCCC ACCCCAACTT GTTTATTGCA 721 GCTTATAATG GTTACAAATA AAGCAATAGC ATCACAAATT TCACAAATAA AGCATTTTTT 781 TCNCTGCATT CTAGTTGTGG TTTGTCCNAA CTCATCAATG TATCTNATCA TGTCTGTATA 841 CCGTCGACCT CTAGCTAGAG CTTGGCGTAA TCATGGTCAT AGCNGTTNCC TGTGTGAAAT 901 TGTTATCCGC TCNCNATTCC NCANNNCATA CNANNCGGNN NNATAAANTG NAAANCCTGG 961 GGGTGNCTAA NGAGNGANCN NNNCACATTA NNNGCNNNGN NNNCNNNNNC NNNNTNNNNN 1021 NNNGNNNNCN NNNNNNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

user report	Nishida, J., Epigenetic remodelling shapes inflammatory renal cancer and neutrophil-dependent metastasis. Nat. Cell Biol. 22 (4): 465-475 (2020). PMID 32203421.
user report	Seki, R., Induced pluripotent stem cell-derived monocytic cell lines from a NOMID patient serve as a screening platform for modulating NLRP3 inflammasome activity. PLoS One 15 (8): e0237030 (2020). PMID 32810141.
user report	Morikawa, M., The ALK-1/SMAD/ATOH8 axis attenuates hypoxic responses and protects against the development of pulmonary arterial hypertension. Sci. Signal. 12(607). pii: eaay4430 (2019). PMID 31719172.
user report	Kambara, H., Gasdermin D Exerts Anti-inflammatory Effects by Promoting Neutrophil Death. Cell Rep. 22 (11): 2924-2936 (2018). PMID 29539421.
user report	Yamamoto, E., Establishment and characterization of cell lines derived from complete hydatidiform mole. Int. J. Mol. Med. 40 (3): 614-622 (2017). PMID 28713902.
user report	Suzuki, T., RUNX1 regulates site specificity of DNA demethylation by recruitment of DNA demethylation machineries in hematopoietic cells. Blood Adv. 1 (20): 1699-1711 (2017). PMID 29296817.
user report	Imamura, Y., Generation of Large Numbers of Antigen-Expressing Human Dendritic Cells Using CD14-ML Technology. PLoS One. 2016 Apr 6;11(4):e0152384 (2016). PMID 27050553.
user report	Inagawa, Y., A human cancer xenograft model utilizing normal pancreatic duct epithelial cells conditionally transformed with defined oncogenes. Carcinogenesis 35 (8): 1840-1846 (2014). PMID 24858378.
user report	Moriyama, H., Tightly regulated and homogeneous transgene expression in human adipose-derived mesenchymal stem cells by lentivirus with tet-off system. PLoS One, 8 (6): e66274 (2013). PMID 23776652.
user report	Yugawa, T., Noncanonical NOTCH signaling limits self-renewal of human epithelial and induced pluripotent stem cells through ROCK activation. Mol. Cell. Biol., 233 (22): 4434-4447 (2013). PMID 24019071.
user report	Yoshita, M., Activation of mTOR by human T-cell leukemia virus type 1 Tax is important for the transformation of mouse T cells to interleukin-2-independent growth. Cancer Sci., 103 (2): 369-374 (2012). PMID 22010857.
user report	Suzuki, T.,, Reconstruction of monocyte transcriptional regulatory network accompanies monocytic functions in human fibroblasts. PLoS One, 7 (3): e33474 (2012). PMID 22428058.
user report	Makino, K., Discoidin domain receptor 2-microRNA 196a-mediated negative feedback against excess type I collagen expression is impaired in scleroderma dermal fibroblasts. J. Invest. Dermatol., 133 (1): 110-119 (2012). PMID 22832484.
user report	Moriyama, M., Human adipose tissue-derived multilineage progenitor cells exposed to oxidative stress induce neurite outgrowth in PC12 cells through p38 MAPK signaling. BMC Cell Biol. 2012 Aug 7;13:21 (2012). PMID 22870983.
user report	Kataoka, A., Involvement of vasodilator-stimulated phosphoprotein in UDP-induced microglial actin aggregation via PKC- and Rho-dependent pathways. Purinergic Signal, 7 (4): 403-411 (2011). PMID 21567128.
user report	Yokomine K., The forkhead box M1 transcription factor as a candidate of target for anti-cancer immunotherapy. Int. J. Cancer., 126, 2153-2163 (2010). PMID 19688828.
user report	Ito S., Expression of a chemokine BRAK/CXCL14 in oral floor carcinoma cells reduces the settlement rate of the cells and suppresses their proliferation in vivo. Biomed. Res., 31, 199-206 (2010). PMID 20622470.
user report	Harao M., HLA-A2-restricted CTL epitopes of a novel lung cancer-associated cancer testis antigen, cell division cycle associated 1, can induce tumor-reactive CTL. Int. J. Cancer., 123, 2616-2625 (2008). PMID 18770861.
user report	Imai K., Identification of a novel tumor-associated antigen, cadherin 3/P-cadherin, as a possible target for immunotherapy of pancreatic, gastric, and colorectal cancers. Clin. Cancer. Res., 14, 6487-6495 (2008). PMID 18927288.
user report	Irie A., Protein kinase D2 contributes to either IL-2 promoter regulation or induction of cell death upon TCR stimulation depending on its activity in Jurkat cells. Int. Immunol., 18, 1737-1747 (2006). PMID 17077180.

2020.12.30

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