Sequencing and PCR primers for primer information.
Backbone vector of lentivirus construct by Gateway(R) cloning with EF-1 alpha promoter.
| Catalog number | RDB04387 |
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| Resource name | CSII-EF-RfA |
| Clone info. | Backbone vector of lentivirus construct by Gateway(R) cloning with EF-1 alpha promoter. Use DB3.1 (Invitrogen) or equivalent to amplify this clone. Commonly requested with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector. |
| Vector backbone | CSII-EF-RfA (Plasmid) |
| Selectable markers | Amp^r, Cm^r, ccdB (E. coli); Zeo^r (plasmid transfection of mammalian cells). |
| Originator | Miyoshi, Hiroyuki | |
| Terms and conditions for distribution | The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe Dr. Hiroyuki Miyoshi (The Developer, the current address: Keio University) as the Developer. Dr. Miyoshi may request the RECIPIENT to report on progress and/or results obtained through the use of the BIOLOGICAL RESOURCE. |
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| Ordering | Please visit Ordering instruction. Order form [Credit Card Payment] [Bank Transfer or Check Payment] Material Transfer Agreement (MTA) [Word] |
| Remarks | Remember that you will be working with samples containing infectious virus. Use Survival2 (Invitrogen) or equivalent to amplify this clone. |
[open/close]| Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
|---|---|---|---|
| RDB04387 | CSII-EF-RfA | DNA solution |
Electronic file
| Electronic file | Sequence RDB04387z.seq from Depositor |
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| Electronic file | PDF RDB04387.pdf from Depositor |
Featured content
References
User reports and related articles (go to bottom)
RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.
Data are summerized on test sheet below.
| Test sheet | RDB12131_A5Ccp1.pdf |
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Nucleotide sequence of a portion of this resource (if available).
| Primer: pAxEF_F1 | RDB12131_A5Cca.seq |
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>04387_12131_A5Cc_pAxEF_F1_D01_10.ab1 1 ATGTTTTCTT CATTTCAGGT GTCGTGACAC GCTACCGGTC TCGAGAATTA TCACAAGTTT 61 GTACAAAAAA GCTGAACGAG AAACGTAAAA TGATATAAAT ATCAATATAT TAAATTAGAT 121 TTTGCATAAA AAACAGACTA CATAATACTG TAAAACACAA CATATCCAGT CACTATGGCG 181 GCCGCATTAG GCACCCCAGG CTTTACACTT TATGCTTCCG GCTCGTATAA TGTGTGGATT 241 TTGAGTTAGG ATCCGTCGAG ATTTTCAGGA GCTAAGGAAG CTAAAATGGA GAAAAAAATC 301 ACTGGATATA CCACCGTTGA TATATCCCAA TGGCATCGTA AAGAACATTT TGAGGCATTT 361 CAGTCAGTTG CTCAATGTAC CTATAACCAG ACCGTTCAGC TGGATATTAC GGCCTTTTTA 421 AAGACCGTAA AGAAAAATAA GCACAAGTTT TATCCGGCCT TTATTCACAT TCTTGCCCGC 481 CTGATGAATG CTCATCCGGA ATTCCGTATG GCAATGAAAG ACGGTGAGCT GGTGATATGG 541 GATAGTGTTC ACCCTTGTTA CACCGTTTTC CATGAGCAAA CTGAAACGTT TTCATCGCTC 601 TGGAGTGAAT ACCACGACGA TTTCCGGCAG TTTCTACACA TATATTCGCA AGATGTGGCG 661 TGTTACGGTG AAAACCTGGC CTATTTCCCT AAAGGGTTTA TTGAGAATAT GTTTTTCGTC 721 TCAGCCAATC CCTGGGTGAG TTTCACCAGT TTTGATTTAA ACGTGGCCAA TATGGACAAC 781 TTCTTCGCCC CCGTTTTCAC CATGGGCAAA TATTATACGC AAGGCGACAA GGTGCTGATG 841 CCGCTGGCGA TTCAGGTTCA TCATGCCGTT TGTGATGGCT TCCATGTCGG CAGAATGCTT 901 AATGAATTAC AACAGTACTG CGATGAGTGG CAGGGCGGGG CGTAAACGCG TGGATCCGGC 961 TTACTAAAAG CCAGATAACA GTATGCGTAT TTGCGCGCTG ATTTTTGCGG TATAAGAATA 1021 TATACTGATA TGTATACCCG AAGTATGTCA AAAAGAGGTA TGCCTATGAA GCAGCGTATT 1081 AACAGTGACA GCTGACAGCG ACCAGGCCTA TCCAGGCTTG CTCAAGGTCA ATATTAAGTG 1141 CATGGTTCCA TTAATT // |
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| Primer: ccdB-C_F | RDB12131_A5Ccb.seq |
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>04387_12131_A5Cc_ccdB-C_F_E01_13.ab1 1 GTACGGGCGG GTGCATATCG GGGATGAAGC TGGCGCATGA TGACCACCGA TATGGCCAGT 61 GTGCCGGTCT CCGTTATCGG GGAAGAAGTG GCTGATCTCA GCCACCGCGA AAATGACATC 121 AAAAACGCCA TTAACCTGAT GTTCTGGGGA ATATAAATGT CAGGCTCCCT TATACACAGC 181 CAGTCTGCAG GTCGACCATA GTGACTGGAT ATGTTGTGTT TTACAGTATT ATGTAGTCTG 241 TTTTTTATGC AAAATCTAAT TTAATATATT GATATTTATA TCATTTTACG TTTCTCGTTC 301 AGCTTTCTTG TACAAAGTGG TGATAATTCT GCAGCGGCCG CGGATCCTCT AGAGTTAACA 361 TCGAGGGATC AAGCTTATCG ATAATCAACC TCTGGATTAC AAAATTTGTG AAAGATTGAC 421 TGGTATTCTT AACTATGTTG CTCCTTTTAC GCTATGTGGA TACGCTGCTT TAATGCCTTT 481 GTATCATGCT ATTGCTTCCC GTATGGCTTT CATTTTCTCC TCCTTGTATA AATCCTGGTT 541 GCTGTCTCTT TATGAGGAGT TGTGGCCCGT TGTCAGGCAA CGTGGCGTGG TGTGCACTGT 601 GTTTGCTGAC GCAACCCCCA CTGGTTGGGG CATTGCCACC ACCTGTCAGC TCCTTTCCGG 661 GACTTTCGCT TTCCCCCTCC CTATTGCCAC GGCGGAACTC ATCGCCGCCT GCCTTGCCCG 721 CTGCTGGACA GGGGCTCGGC TGTTGGGCAC TGACAATTCC GTGGTGTTGT CGGGGAAGCT 781 GACGTCCTTT CCATGGCTGC TCGCCTGTGT TGCCACCTGG ATTCTGCGCG GGACGTCCTT 841 CTGCTACGTC CCTTCGGCCC TCAATCCAGC GGACCTTCCT TCCCGCGGCC TGCTGCCGGC 901 TCTGCGGGCC TCTTCCGCGT CTTCGCCTTC GCCCTCAGAC GAGTCGGATC TCCCTTTGGG 961 CCGCCTTCCC CCGCATCGAT ACCGTCGAGA CCTAGAAAAA CATGGAGCAA TCACAGGTAG 1021 CATACAGCAG CTACCATGCT GATGTGCTTG CTAGAGCACA GAGAAGAGAG TTGTATTCAG 1081 TCACACCTCA AGTACCTAGA ACCATGACTT ACAGGCAGCT GTAGATCTTA GCCCAACTTT 1141 TTCAGAGGA // |
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| Primer: CMV-Forward | RDB12131_A5Ccc.seq |
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>04387_12131_A5Cc_CMV-Forwaed_F01_16.ab1 1 CTTCACCTCC GCCCATTGAC GCAATGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA 61 GCAGCGCGTT TTGCCTGTAC TGGGTCTCTC TGGTTAGACC AGATCTGAGC CTGGGAGCTC 121 TCTGGCTAAC TAGGGAACCC ACTGCTTAAG CCTCAATAAA GCTTGCCTTG AGTGCTTCAA 181 GTAGTGTGTG CCCGTCTGTT GTGTGACTCT GGTAACTAGA GATCCCTCAG ACCCTTTTAG 241 TCAGTGTGGA AAATCTCTAG CAGTGGCGCC CGAACAGGGA CTTGAAAGCG AAAGGGAAAC 301 CAGAGGAGCT CTCTCGACGC AGGACTCGGC TTGCTGAAGC GCGCACGGCA AGAGGCGAGG 361 GGCGGCGACT GGTGAGTACG CCAAAAATTT TGACTAGCGG AGGCTAGAAG GAGAGAGATG 421 GGTGCGAGAG CGTCAGTATT AAGCGGGGGA GAATTAGATC GCGATGGGAA AAAATTCGGT 481 TAAGGCCAGG GGGAAAGAAA AAATATAAAT TAAAACATAT AGTATGGGCA AGCAGGGAGC 541 TAGAACGATT CGCAGTTAAT CCTGGCCTGT TAGAAACATC AGAAGGCTGT AGACAAATAC 601 TGGGACAGCT ACAACCATCC CTTCAGACAG GATCAGAAGA ACTTAGATCA TTATATAATA 661 CAGTAGCAAC CCTCTATTGT GTGCATCAAA GGATAGAGAT AAAAGACACC AAGGAAGCTT 721 TAGACAAGAT AGAGGAAGAG CAAAACAAAA GTAAGACCAC CGCACAGCAA GGGCCGCTGA 781 TCTTCAGACC TGGAGGAGGA GATATGAGGG ACAATTGGAG AAGTGAATTA TTATAAATAA 841 TAAGTAGTAA AAATTGAAAC CATTAGGAGT AGCACCCACC AAGGCAAAGA GAAGAAGTGG 901 GTGCAGAGAA GAAAAAAAAG AGCAGTGGGA ATAGGAGCTT TGTTTCTTGG ATTCTTTGGG 961 AGCAGCAGGA GCACTATGGG GCGCACGGTC ATGACGCTGA CCGTACAGCC AGACAAATTA 1021 TTTGTCTGCA TAGTGCAGCG CGAACCATCG TGAGGCTATG AGGCCGCAGC ATCTGTGCAC 1081 TCAAGTCTGG GCACTCAAGC AGCTCG // |
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| Primer: BGH_rev | RDB12131_A5Ccd.seq |
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>04387_12131_A5Cc_BGHrev_G01_19.ab1 1 GTTCGGACTG CTAGAGATTT TCCACACTGA CTAAAAGGGT CTGAGGGATC TCTAGTTACC 61 AGAGTCACAC AACAGACGGG CACACACTAC TTGAAGCACT CAAGGCAAGC TTTATTGAGG 121 CTTAAGCAGT GGGTTCCCTA GTTAGCCAGA GAGCTCCCAG GCTCAGATCT GGTCTAACCA 181 GAGAGACCCA GTACAGTCCG GATGCAGCTC TCGGGCCATG TGATGAAATG CTAGGCGGCT 241 GTCAAACCTC CACTCTAATA CTTCTCTCTC CGGGTCATCC ATCCCATGCA GGCTCACAGG 301 GTGTAACAAG CGGGTGTTCT CTCCTTCATT GGCTTCTTCT ACCTTCTCTT GCTCAACTGG 361 TACTAGCTTG TAGCACCATC CAAAGGTCAG TGGATATCTG ATCCCTGGCC CTGGTGTGTA 421 GTTCTGCCAA TCAGGGAAGT AGCCTTGTGT GTGGTAGATC CACAGATCAA GGATATCTTG 481 TCTTCGTTGG GAGTGAATTA GCCCTTCCAG TCCCCCCTTT TCTTTTAAAA AGTGGCTAAG 541 ATCTACAGCT GCCTTGTAAG TCATTGGTCT TAAAGGTACC TGAGGTGTGA CTGGAAAACC 601 CACCTCCTCC TCCTCTTGTG CTTCTAGCCA GGCACAATCA GCATTGGTAG CTGCTGTATT 661 GCTACTTGTG ATTGCTCCAT GTTTTTCTAG GTCTCGACGG TATCGATGCG GGGAGGCGGC 721 CCAAAGGGAG ATCCGACTCG TCTGAGGGCG AAGGCGAAGA CGCGGAAGAG GCCGCAGAGC 781 CGGCAGCAGG CCGCGGGAAG GAAGGTCCGC TGGATTGAGG GCCGAAGGGA CGTAGCAGAA 841 GGACGTCCCG CGCAGAATCC AGGTGGCAAC ACAGGCGAGC AGCCATGGAA AGGACGTCAG 901 CTTCCCCGAC AACACCACGG AATTGTCAGT GCCCAACAGC CGAGCCCCTG TCCAGCAGCG 961 GGCAAAGGCA GGCGGCGATG AGTTCCGCCG TGGCAATAGG GAGGGGGAAG CGAAAGTCCC 1021 GGAAGGAGCT GACAGGTGGT GGCATGCCCC ACAGTGGGGG GTTGCGTCAG CCAAACACAG 1081 TGCACCACCA CGACACGTTG CCTGACGACG GAGCACACTC GTCATAAGAA GACAGCACCA 1141 GGAT // |
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Please visit Sequencing and PCR primers for primer information.
References
2018.10.22
GNP_filter3_RDBDEP_html_181012.pl