Backbone vector plasmid of lentivirus construct by Gateway(R) cloning with EF-1 alpha promoter.
Catalog number | RDB04387 |
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Resource name | CSII-EF-RfA |
Clone info. | Backbone vector plasmid of lentivirus construct by Gateway(R) cloning with EF-1 alpha promoter. Commonly requested with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector. |
Vector backbone | CSII-EF-RfA (Plasmid) |
Selectable markers | Please note that Zeo resistance marker is not included in the lentivirus produced from this vector. E. coli: Amp^r, Cm^r, ccdB. |
Growth remarks | Use DB3.1, ccdB Survival or equivalent to amplify this clone. 30 degC is fine. |
Depositor|Developer | Miyoshi, Hiroyuki | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR | The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer. |
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Ordering | Please visit Ordering instruction.[link] Order form [Credit Card Payment ![]() ![]() Material Transfer Agreement (MTA, for use for not-for-profit academic purpose)[Word ![]() |
Remarks | Remember that you will be working with samples containing infectious virus. Use Survival2 (Invitrogen) or equivalent to amplify this clone. |
Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
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RDB04387 | CSII-EF-RfA | DNA solution |
Electronic file
Electronic file | Sequence RDB04387z.seq |
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Electronic file | Remarks, protocol and/or map (pdf) RDB04387.pdf |
Original reference
Further references such as user reports and related articles (go to bottom)
Featured content
RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.
Test sheet | RDB12131_A2H6p1-3.pdf ![]() |
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Nucleotide sequence of a portion of this resource (if available).
Primer: EF1a-C_F Region: EF-1alpha pro,RfA(attR1,lacUV5 pro,CmR) Sequence file: RDB12131_A2H6e.seq ![]() |
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>04387_04387_A2H6_EF1a-C_F_C05_08_ABI24.ab1 1 NNNNNNNNNN NNNNTTTTGA NTTTGGNNCT TGGTTNNTTC TCNAGCCTCA GACAGTGGTT 61 CAAAGTTTTT TTCTTCCATT TCAGGTGTCG TGAACACGCT ACCGGTCTCG AGAATTATCA 121 CAAGTTTGTA CAAAAAAGCT GAACGAGAAA CGTAAAATGA TATAAATATC AATATATTAA 181 ATTAGATTTT GCATAAAAAA CAGACTACAT AATACTGTAA AACACAACAT ATCCAGTCAC 241 TATGGCGGCC GCATTAGGCA CCCCAGGCTT TACACTTTAT GCTTCCGGCT CGTATAATGT 301 GTGGATTTTG AGTTAGGATC CGTCGAGATT TTCAGGAGCT AAGGAAGCTA AAATGGAGAA 361 AAAAATCACT GGATATACCA CCGTTGATAT ATCCCAATGG CATCGTAAAG AACATTTTGA 421 GGCATTTCAG TCAGTTGCTC AATGTACCTA TAACCAGACC GTTCAGCTGG ATATTACGGC 481 CTTTTTAAAG ACCGTAAAGA AAAATAAGCA CAAGTTTTAT CCGGCCTTTA TTCACATTCT 541 TGCCCGCCTG ATGAATGCTC ATCCGGAATT CCGTATGGCA ATGAAAGACG GTGAGCTGGT 601 GATATGGGAT AGTGTTCACC CTTGTTACAC CGTTTTCCAT GAGCAAACTG AAACGTTTTC 661 ATCGCTCTGG AGTGAATACC ACGACGATTT CCGGCAGTTT CTACACATAT ATTCGCAAGA 721 TGTGGCGTGT TACGGTGAAA ACCTGNCCTA TTTCCCTAAA GGGTTTATTG AGAATATGTT 781 TTTCGTCTCA GCCAATCCCT GGGTGAGTTT CACCAGTTTT GATTTAAACG TGGCCNATAT 841 GGACAACTTC TTCGCCCCCG TTTTCACCAN GGGCNAATAT TATACGCNAG GCGNNNANGT 901 GCTGANGNCG CTGGCNANTC NNNNCATCAT GCCNTTNGNG ATGGNNTCCA NGTCGGCNNA 961 ATGCTTNNTG NNNTACNANN NNANNGCNNT GANNNNNNNN NN // |
Primer: ccdB-C_F Region: RfA(ccdB,attR2),PRE Sequence file: RDB12131_A2H6f.seq ![]() |
>04387_04387_A2H6_ccdB-C_F_D05_11_ABI24.ab1 1 NNNNNNNNNN NNNNNTCGGG GNNGAANCTG GCGCATGATG ACCNCCGATA TGGCCAGTGT 61 GCCGGTCTCC GTTATCGGGG AAGAAGTGGC TGATCTCAGC CACCGCGAAA ATGACATCAA 121 AAACGCCATT AACCTGATGT TCTGGGGAAT ATAAATGTCA GGCTCCCTTA TACACAGCCA 181 GTCTGCAGGT CGACCATAGT GACTGGATAT GTTGTGTTTT ACAGTATTAT GTAGTCTGTT 241 TTTTATGCAA AATCTAATTT AATATATTGA TATTTATATC ATTTTACGTT TCTCGTTCAG 301 CTTTCTTGTA CAAAGTGGTG ATAATTCTGC AGCGGCCGCG GATCCTCTAG AGTTAACATC 361 GAGGGATCAA GCTTATCGAT AATCAACCTC TGGATTACAA AATTTGTGAA AGATTGACTG 421 GTATTCTTAA CTATGTTGCT CCTTTTACGC TATGTGGATA CGCTGCTTTA ATGCCTTTGT 481 ATCATGCTAT TGCTTCCCGT ATGGCTTTCA TTTTCTCCTC CTTGTATAAA TCCTGGTTGC 541 TGTCTCTTTA TGAGGAGTTG TGGCCCGTTG TCAGGCAACG TGGCGTGGTG TGCACTGTGT 601 TTGCTGACGC AACCCCCACT GGTTGGGGCA TTGCCACCAC CTGTCAGCTC CTTTCCGGGA 661 CTTTCGCTTT CCCCCTCCCT ATTGCCACGG CGGAACTCAT CGCCGCCTGC CTTGCCCGCT 721 GCTGGACAGG GGCTCGGCTG TTGGGCACTG ACAATTCCGT GGTGTTGTCG GGGAANCTGA 781 CGTCCTTTCC NTGGCTGCTC GCCTGTGTTG CCACCTGGAT TCTGCGCGGG ANGTCCTTCT 841 GCTACGTCCC TTCGGCCNTC NATCCAGCGG ANCTTNCTTN CCGCGGCCTG CTGNNGGNNN 901 TGCGGNNNCT TNNNNGNCTT CNCCTTCGCC CTCAGACGAG TCGGATCNNC NNTTGGGCCN 961 NCNCCCCGCN TCNATANNNN CNNNANNNAN AAAAANNTNN // |
Primer: CMV_Forward Region: CMVpro,5'LTR,HIV-1psi Sequence file: RDB12131_A2H6g.seq ![]() |
>04387_04387_A2H6_CMV_Forward_E05_14_ABI24.ab1 1 NNNNNNNNNN NNNNNTGNNG CNNNGGGCGG TAGGCGTGTA CGGTGGGAGG TCTATATAAG 61 CAGCGCGTTT TGCCTGTACT GGGTCTCTCT GGTTAGACCA GATCTGAGCC TGGGAGCTCT 121 CTGGCTAACT AGGGAACCCA CTGCTTAAGC CTCAATAAAG CTTGCCTTGA GTGCTTCAAG 181 TAGTGTGTGC CCGTCTGTTG TGTGACTCTG GTAACTAGAG ATCCCTCAGA CCCTTTTAGT 241 CAGTGTGGAA AATCTCTAGC AGTGGCGCCC GAACAGGGAC TTGAAAGCGA AAGGGAAACC 301 AGAGGAGCTC TCTCGACGCA GGACTCGGCT TGCTGAAGCG CGCACGGCAA GAGGCGAGGG 361 GCGGCGACTG GTGAGTACGC CAAAAATTTT GACTAGCGGA GGCTAGAAGG AGAGAGATGG 421 GTGCGAGAGC GTCAGTATTA AGCGGGGGAG AATTAGATCG CGATGGGAAA AAATTCGGTT 481 AAGGCCAGGG GGAAAGAAAA AATATAAATT AAAACATATA GTATGGGCAA GCAGGGAGCT 541 AGAACGATTC GCAGTTAATC CTGGCCTGTT AGAAACATCA GAAGGCTGTA GACAAATACT 601 GGGACAGCTA CAACCATCCC TTCAGACAGG ATCAGAAGAA CTTAGATCAT TATATAATAC 661 AGTAGCAACC CTCTATTGTG TGCATCAAAG GATAGAGATA AAAGACACCA AGGAAGCTTT 721 AGACAAGATA GAGGAAGAGC AAAACAAAAG TAAGANCACC GCACAGCAAG GGCCGCTGAT 781 CTTCAGACNT GGAGGANGNN NTATGANGGA NAATTGGAGA AGTGAATTAT ATAAATATAA 841 AGTAGTAAAA ATTGAACCNT TANGAGTAGC ACNNNCNNAN GNNNNGAGAA NANNGGTGCA 901 GANAGAAAAA AAGANCAGNG GGAATAGGAN NTTTGTNNCT NNGGGNNCNN GGNNNCAGCN 961 NNAANCNCTA NNGGNNNNNN NNNN // |
Primer: cPPT/CTS-E_R Region: cPPT_CTS,RRE Sequence file: RDB12131_A2H6h.seq ![]() |
>04387_04387_A2H6_cPPT_CTS-E_R_F05_17_ABI24.ab1 1 NNNNNNNNNT NGNTTTTNNN NNCTTTAGTT TGTATGTCTG TTGCTATTAT GTCTACTATT 61 CTTTCCCCTG CACTGTACCC CCCAATCCCC CCTTTTCTTT TAAAATTGTG GATGAATACT 121 GCCATTTGTA GATCCGTTCA CTAATCGAAT GGATCTGTCT CTGTCTCTCT CTCCACCTTC 181 TTCTTCTATT CCTTCGGGCC TGTCGGGTCC CCTCGGGGTT GGGAGGTGGG TCTGAAACGA 241 TAATGGTGAA TATCCCTGCC TAACTCTATT CACTATAGAA AGTACAGCAA AAACTATTCT 301 TAAACCTACC AAGCCTCCTA CTATCATTAT GAATAATTTT ATATACCACA GCCAATTTGT 361 TATGTTAAAC CAATTCCACA AACTTGCCCA TTTATCTAAT TCCAATAATT CTTGTTCATT 421 CTTTTCTTGC TGGTTTTGCG ATTCTTCAAT TAAGGAGTGT ATTAAGCTTG TGTAATTGTT 481 AATTTCTCTG TCCCACTCCA TCCAGGTCGT GTGATTCCAA ATCTGTTCCA GAGATTTATT 541 ACTCCAACTA GCATTCCAAG GCACAGCAGT GGTGCAAATG AGTTTTCCAG AGCAACCCCA 601 AATCCCCAGG AGCTGTTGAT CCTTTAGGTA TCTTTCCACA GCCAGGATTC TTGCCTGGAG 661 CTGCTTGATG CCCCAGACTG TGAGTTGCAA CAGATGCTGT TGCGCCTCAA TAGCCCTCAG 721 CAAATTGTTC TGCTGCTGCA CTATACCAGA CAATAATTGT CTGGCCTGTA NCGTCAGCGT 781 CATTGACGCT GCGCNCATAG TGCTTCCTGC TGCTCNNNAG AACCCAAGGA ACAAAGCTCC 841 TATTCCCACT GCTCTTTTTT TCTCTCTGCA CCACTCTTCT CTTTGCCTNG GNGGGTGCTA 901 CTCCNAANGG TTCAATTTTN NCTACTTTAT ATTTATATAN NTCACTTCTN NNATTGTCCN 961 NCATANCNNC NNNNNCANNN NNNNNNN // |
Primer: bGH-B_R Region: 3'LTR,PRE Sequence file: RDB12131_A2H6i.seq ![]() |
>04387_04387_A2H6_bGH-B_R_G05_20_ABI24.ab1 1 NNNNNNNNNN NNNNTNNNNN GCGGGTNNNN NNGGCCCTGC TAGAGANTTT CCNCACTGAC 61 TAAAAGGGTC TGAGGGATCT CTAGTTACCA GAGTCACACA ACAGACGGGC ACACACTACT 121 TGAAGCACTC AAGGCAAGCT TTATTGAGGC TTAAGCAGTG GGTTCCCTAG TTAGCCAGAG 181 AGCTCCCAGG CTCAGATCTG GTCTAACCAG AGAGACCCAG TACAGTCCGG ATGCAGCTCT 241 CGGGCCATGT GATGAAATGC TAGGCGGCTG TCAAACCTCC ACTCTAATAC TTCTCTCTCC 301 GGGTCATCCA TCCCATGCAG GCTCACAGGG TGTAACAAGC GGGTGTTCTC TCCTTCATTG 361 GCTTCTTCTA CCTTCTCTTG CTCAACTGGT ACTAGCTTGT AGCACCATCC AAAGGTCAGT 421 GGATATCTGA TCCCTGGCCC TGGTGTGTAG TTCTGCCAAT CAGGGAAGTA GCCTTGTGTG 481 TGGTAGATCC ACAGATCAAG GATATCTTGT CTTCGTTGGG AGTGAATTAG CCCTTCCAGT 541 CCCCCCTTTT CTTTTAAAAA GTGGCTAAGA TCTACAGCTG CCTTGTAAGT CATTGGTCTT 601 AAAGGTACCT GAGGTGTGAC TGGAAAACCC ACCTCCTCCT CCTCTTGTGC TTCTAGCCAG 661 GCACAATCAG CATTGGTAGC TGCTGTATTG CTACTTGTGA TTGCTCCATG TTTTTCTAGG 721 TCTCGACGGT ATCGATGCGG GGAGGCGGCC CAAAGGGAGA TCCGACTCGT CTGANGGCGA 781 ANGCGAAGAN GCGGAANANG CCGCAGANCC GGCAGCANGC CGCGNNAANG AANGTCCGCT 841 GGATTGAGGG CCGAANGGAC GTAGCAGAAN GANGTCCCGC GCAGAATCCA GGTGGCAACA 901 CANGCNANCA GCCNTGGAAA GGNNGTCAGC TTCCCCNNNN ACACCNCNNN NNTGNCAGNG 961 CCNNACAGNC NANCCNNNNN NNNNCANCGG NNNANNNNNN NNNN // |
Primer: SV40pro_ori_F Region: SV40pro_ori,Zeo,SV40p(A) Sequence file: RDB12131_A2H6j.seq ![]() |
>04387_04387_A2H6_SV40pro_ori_F_H05_23_ABI24.ab1 1 NNNNNNNNNN NNNNNNNANG NCGCCTCTGC CTCTGANCTA TTCNNGAAGT AGTGAGGAGG 61 CTTTTTTGGA GGCCTAGGCT TTTGCAAAAA GCTCCCGGGA GCTTGTATAT CCATTTTCGG 121 ATCTGATCAG CACGTGTTGA CAATTAATCA TCGGCATAGT ATATCGGCAT AGTATAATAC 181 GACAAGGTGA GGAACTAAAC CATGGCCAAG TTGACCAGTG CCGTTCCGGT GCTCACCGCG 241 CGCGACGTCG CCGGAGCGGT CGAGTTCTGG ACCGACCGGC TCGGGTTCTC CCGGGACTTC 301 GTGGAGGACG ACTTCGCCGG TGTGGTCCGG GACGACGTGA CCCTGTTCAT CAGCGCGGTC 361 CAGGACCAGG TGGTGCCGGA CAACACCCTG GCCTGGGTGT GGGTGCGCGG CCTGGACGAG 421 CTGTACGCCG AGTGGTCGGA GGTCGTGTCC ACGAACTTCC GGGACGCCTC CGGGCCGGCC 481 ATGACCGAGA TCGGCGAGCA GCCGTGGGGG CGGGAGTTCG CCCTGCGCGA CCCGGCCGGC 541 AACTGCGTGC ACTTCGTGGC CGAGGAGCAG GACTGACACG TGCTACGAGA TTTCGATTCC 601 ACCGCCGCCT TCTATGAAAG GTTGGGCTTC GGAATCGTTT TCCGGGACGC CGGCTGGATG 661 ATCCTCCAGC GCGGGGATCT CATGCTGGAG TTCTTCGCCC ACCCCAACTT GTTTATTGCA 721 GCTTATAATG GTTACAAATA AAGCAATAGC ATCACAAATT TCACAAATAA AGCATTTTTT 781 TCNCTGCATT CTAGTTGTGG TTTGTCCNAA CTCATCAATG TATCTNATCA TGTCTGTATA 841 CCGTCGACCT CTAGCTAGAG CTTGGCGTAA TCATGGTCAT AGCNGTTNCC TGTGTGAAAT 901 TGTTATCCGC TCNCNATTCC NCANNNCATA CNANNCGGNN NNATAAANTG NAAANCCTGG 961 GGGTGNCTAA NGAGNGANCN NNNCACATTA NNNGCNNNGN NNNCNNNNNC NNNNTNNNNN 1021 NNNGNNNNCN NNNNNNNNN // |
Please visit Sequencing and PCR primers for primer information.
Original, user report and related articles
2020.12.30
GNP_filter3_RDBDEP_html_201222.pl