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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

CSII-EF-RfA

Backbone vector plasmid of lentivirus construct by Gateway(R) cloning with EF-1 alpha promoter.

Catalog number RDB04387
Resource name CSII-EF-RfA
Clone info. Backbone vector plasmid of lentivirus construct by Gateway(R) cloning with EF-1 alpha promoter.
Commonly requested with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Vector backbone CSII-EF-RfA (Plasmid)
Selectable markers Please note that Zeo resistance marker is not included in the lentivirus produced from this vector. E. coli: Amp^r, Cm^r, ccdB.
Growth remarks Use DB3.1, ccdB Survival or equivalent to amplify this clone. 30 degC is fine.
Depositor|Developer Miyoshi, Hiroyuki |
Lentiviral plasmid list, maps and sequences information. [link]

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer.
Ordering Please visit Ordering instruction.[link] 
Order form 
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Material Transfer Agreement (MTA, for use for not-for-profit academic purpose)[Word]
Remarks Remember that you will be working with samples containing infectious virus.
Use Survival2 (Invitrogen) or equivalent to amplify this clone.
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB04387 CSII-EF-RfA DNA solution

Ordering instruction of plasmids [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Electronic file Sequence RDB04387z.seq
Electronic file Remarks, protocol and/or map (pdf) RDB04387.pdf

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content


Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB12131_A2H6p1-3.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: EF1a-C_F
Region: EF-1alpha pro,RfA(attR1,lacUV5 pro,CmR)
Sequence file: RDB12131_A2H6e.seq check
>04387_04387_A2H6_EF1a-C_F_C05_08_ABI24.ab1
    1 NNNNNNNNNN NNNNTTTTGA NTTTGGNNCT TGGTTNNTTC TCNAGCCTCA GACAGTGGTT
   61 CAAAGTTTTT TTCTTCCATT TCAGGTGTCG TGAACACGCT ACCGGTCTCG AGAATTATCA
  121 CAAGTTTGTA CAAAAAAGCT GAACGAGAAA CGTAAAATGA TATAAATATC AATATATTAA
  181 ATTAGATTTT GCATAAAAAA CAGACTACAT AATACTGTAA AACACAACAT ATCCAGTCAC
  241 TATGGCGGCC GCATTAGGCA CCCCAGGCTT TACACTTTAT GCTTCCGGCT CGTATAATGT
  301 GTGGATTTTG AGTTAGGATC CGTCGAGATT TTCAGGAGCT AAGGAAGCTA AAATGGAGAA
  361 AAAAATCACT GGATATACCA CCGTTGATAT ATCCCAATGG CATCGTAAAG AACATTTTGA
  421 GGCATTTCAG TCAGTTGCTC AATGTACCTA TAACCAGACC GTTCAGCTGG ATATTACGGC
  481 CTTTTTAAAG ACCGTAAAGA AAAATAAGCA CAAGTTTTAT CCGGCCTTTA TTCACATTCT
  541 TGCCCGCCTG ATGAATGCTC ATCCGGAATT CCGTATGGCA ATGAAAGACG GTGAGCTGGT
  601 GATATGGGAT AGTGTTCACC CTTGTTACAC CGTTTTCCAT GAGCAAACTG AAACGTTTTC
  661 ATCGCTCTGG AGTGAATACC ACGACGATTT CCGGCAGTTT CTACACATAT ATTCGCAAGA
  721 TGTGGCGTGT TACGGTGAAA ACCTGNCCTA TTTCCCTAAA GGGTTTATTG AGAATATGTT
  781 TTTCGTCTCA GCCAATCCCT GGGTGAGTTT CACCAGTTTT GATTTAAACG TGGCCNATAT
  841 GGACAACTTC TTCGCCCCCG TTTTCACCAN GGGCNAATAT TATACGCNAG GCGNNNANGT
  901 GCTGANGNCG CTGGCNANTC NNNNCATCAT GCCNTTNGNG ATGGNNTCCA NGTCGGCNNA
961 ATGCTTNNTG NNNTACNANN NNANNGCNNT GANNNNNNNN NN
//
Primer: ccdB-C_F
Region: RfA(ccdB,attR2),PRE
Sequence file: RDB12131_A2H6f.seq check
>04387_04387_A2H6_ccdB-C_F_D05_11_ABI24.ab1
    1 NNNNNNNNNN NNNNNTCGGG GNNGAANCTG GCGCATGATG ACCNCCGATA TGGCCAGTGT
   61 GCCGGTCTCC GTTATCGGGG AAGAAGTGGC TGATCTCAGC CACCGCGAAA ATGACATCAA
  121 AAACGCCATT AACCTGATGT TCTGGGGAAT ATAAATGTCA GGCTCCCTTA TACACAGCCA
  181 GTCTGCAGGT CGACCATAGT GACTGGATAT GTTGTGTTTT ACAGTATTAT GTAGTCTGTT
  241 TTTTATGCAA AATCTAATTT AATATATTGA TATTTATATC ATTTTACGTT TCTCGTTCAG
  301 CTTTCTTGTA CAAAGTGGTG ATAATTCTGC AGCGGCCGCG GATCCTCTAG AGTTAACATC
  361 GAGGGATCAA GCTTATCGAT AATCAACCTC TGGATTACAA AATTTGTGAA AGATTGACTG
  421 GTATTCTTAA CTATGTTGCT CCTTTTACGC TATGTGGATA CGCTGCTTTA ATGCCTTTGT
  481 ATCATGCTAT TGCTTCCCGT ATGGCTTTCA TTTTCTCCTC CTTGTATAAA TCCTGGTTGC
  541 TGTCTCTTTA TGAGGAGTTG TGGCCCGTTG TCAGGCAACG TGGCGTGGTG TGCACTGTGT
  601 TTGCTGACGC AACCCCCACT GGTTGGGGCA TTGCCACCAC CTGTCAGCTC CTTTCCGGGA
  661 CTTTCGCTTT CCCCCTCCCT ATTGCCACGG CGGAACTCAT CGCCGCCTGC CTTGCCCGCT
  721 GCTGGACAGG GGCTCGGCTG TTGGGCACTG ACAATTCCGT GGTGTTGTCG GGGAANCTGA
  781 CGTCCTTTCC NTGGCTGCTC GCCTGTGTTG CCACCTGGAT TCTGCGCGGG ANGTCCTTCT
  841 GCTACGTCCC TTCGGCCNTC NATCCAGCGG ANCTTNCTTN CCGCGGCCTG CTGNNGGNNN
  901 TGCGGNNNCT TNNNNGNCTT CNCCTTCGCC CTCAGACGAG TCGGATCNNC NNTTGGGCCN
961 NCNCCCCGCN TCNATANNNN CNNNANNNAN AAAAANNTNN
//
Primer: CMV_Forward
Region: CMVpro,5'LTR,HIV-1psi
Sequence file: RDB12131_A2H6g.seq check
>04387_04387_A2H6_CMV_Forward_E05_14_ABI24.ab1
    1 NNNNNNNNNN NNNNNTGNNG CNNNGGGCGG TAGGCGTGTA CGGTGGGAGG TCTATATAAG
   61 CAGCGCGTTT TGCCTGTACT GGGTCTCTCT GGTTAGACCA GATCTGAGCC TGGGAGCTCT
  121 CTGGCTAACT AGGGAACCCA CTGCTTAAGC CTCAATAAAG CTTGCCTTGA GTGCTTCAAG
  181 TAGTGTGTGC CCGTCTGTTG TGTGACTCTG GTAACTAGAG ATCCCTCAGA CCCTTTTAGT
  241 CAGTGTGGAA AATCTCTAGC AGTGGCGCCC GAACAGGGAC TTGAAAGCGA AAGGGAAACC
  301 AGAGGAGCTC TCTCGACGCA GGACTCGGCT TGCTGAAGCG CGCACGGCAA GAGGCGAGGG
  361 GCGGCGACTG GTGAGTACGC CAAAAATTTT GACTAGCGGA GGCTAGAAGG AGAGAGATGG
  421 GTGCGAGAGC GTCAGTATTA AGCGGGGGAG AATTAGATCG CGATGGGAAA AAATTCGGTT
  481 AAGGCCAGGG GGAAAGAAAA AATATAAATT AAAACATATA GTATGGGCAA GCAGGGAGCT
  541 AGAACGATTC GCAGTTAATC CTGGCCTGTT AGAAACATCA GAAGGCTGTA GACAAATACT
  601 GGGACAGCTA CAACCATCCC TTCAGACAGG ATCAGAAGAA CTTAGATCAT TATATAATAC
  661 AGTAGCAACC CTCTATTGTG TGCATCAAAG GATAGAGATA AAAGACACCA AGGAAGCTTT
  721 AGACAAGATA GAGGAAGAGC AAAACAAAAG TAAGANCACC GCACAGCAAG GGCCGCTGAT
  781 CTTCAGACNT GGAGGANGNN NTATGANGGA NAATTGGAGA AGTGAATTAT ATAAATATAA
  841 AGTAGTAAAA ATTGAACCNT TANGAGTAGC ACNNNCNNAN GNNNNGAGAA NANNGGTGCA
  901 GANAGAAAAA AAGANCAGNG GGAATAGGAN NTTTGTNNCT NNGGGNNCNN GGNNNCAGCN
961 NNAANCNCTA NNGGNNNNNN NNNN
//
Primer: cPPT/CTS-E_R
Region: cPPT_CTS,RRE
Sequence file: RDB12131_A2H6h.seq check
>04387_04387_A2H6_cPPT_CTS-E_R_F05_17_ABI24.ab1
    1 NNNNNNNNNT NGNTTTTNNN NNCTTTAGTT TGTATGTCTG TTGCTATTAT GTCTACTATT
   61 CTTTCCCCTG CACTGTACCC CCCAATCCCC CCTTTTCTTT TAAAATTGTG GATGAATACT
  121 GCCATTTGTA GATCCGTTCA CTAATCGAAT GGATCTGTCT CTGTCTCTCT CTCCACCTTC
  181 TTCTTCTATT CCTTCGGGCC TGTCGGGTCC CCTCGGGGTT GGGAGGTGGG TCTGAAACGA
  241 TAATGGTGAA TATCCCTGCC TAACTCTATT CACTATAGAA AGTACAGCAA AAACTATTCT
  301 TAAACCTACC AAGCCTCCTA CTATCATTAT GAATAATTTT ATATACCACA GCCAATTTGT
  361 TATGTTAAAC CAATTCCACA AACTTGCCCA TTTATCTAAT TCCAATAATT CTTGTTCATT
  421 CTTTTCTTGC TGGTTTTGCG ATTCTTCAAT TAAGGAGTGT ATTAAGCTTG TGTAATTGTT
  481 AATTTCTCTG TCCCACTCCA TCCAGGTCGT GTGATTCCAA ATCTGTTCCA GAGATTTATT
  541 ACTCCAACTA GCATTCCAAG GCACAGCAGT GGTGCAAATG AGTTTTCCAG AGCAACCCCA
  601 AATCCCCAGG AGCTGTTGAT CCTTTAGGTA TCTTTCCACA GCCAGGATTC TTGCCTGGAG
  661 CTGCTTGATG CCCCAGACTG TGAGTTGCAA CAGATGCTGT TGCGCCTCAA TAGCCCTCAG
  721 CAAATTGTTC TGCTGCTGCA CTATACCAGA CAATAATTGT CTGGCCTGTA NCGTCAGCGT
  781 CATTGACGCT GCGCNCATAG TGCTTCCTGC TGCTCNNNAG AACCCAAGGA ACAAAGCTCC
  841 TATTCCCACT GCTCTTTTTT TCTCTCTGCA CCACTCTTCT CTTTGCCTNG GNGGGTGCTA
  901 CTCCNAANGG TTCAATTTTN NCTACTTTAT ATTTATATAN NTCACTTCTN NNATTGTCCN
961 NCATANCNNC NNNNNCANNN NNNNNNN
//
Primer: bGH-B_R
Region: 3'LTR,PRE
Sequence file: RDB12131_A2H6i.seq check
>04387_04387_A2H6_bGH-B_R_G05_20_ABI24.ab1
    1 NNNNNNNNNN NNNNTNNNNN GCGGGTNNNN NNGGCCCTGC TAGAGANTTT CCNCACTGAC
   61 TAAAAGGGTC TGAGGGATCT CTAGTTACCA GAGTCACACA ACAGACGGGC ACACACTACT
  121 TGAAGCACTC AAGGCAAGCT TTATTGAGGC TTAAGCAGTG GGTTCCCTAG TTAGCCAGAG
  181 AGCTCCCAGG CTCAGATCTG GTCTAACCAG AGAGACCCAG TACAGTCCGG ATGCAGCTCT
  241 CGGGCCATGT GATGAAATGC TAGGCGGCTG TCAAACCTCC ACTCTAATAC TTCTCTCTCC
  301 GGGTCATCCA TCCCATGCAG GCTCACAGGG TGTAACAAGC GGGTGTTCTC TCCTTCATTG
  361 GCTTCTTCTA CCTTCTCTTG CTCAACTGGT ACTAGCTTGT AGCACCATCC AAAGGTCAGT
  421 GGATATCTGA TCCCTGGCCC TGGTGTGTAG TTCTGCCAAT CAGGGAAGTA GCCTTGTGTG
  481 TGGTAGATCC ACAGATCAAG GATATCTTGT CTTCGTTGGG AGTGAATTAG CCCTTCCAGT
  541 CCCCCCTTTT CTTTTAAAAA GTGGCTAAGA TCTACAGCTG CCTTGTAAGT CATTGGTCTT
  601 AAAGGTACCT GAGGTGTGAC TGGAAAACCC ACCTCCTCCT CCTCTTGTGC TTCTAGCCAG
  661 GCACAATCAG CATTGGTAGC TGCTGTATTG CTACTTGTGA TTGCTCCATG TTTTTCTAGG
  721 TCTCGACGGT ATCGATGCGG GGAGGCGGCC CAAAGGGAGA TCCGACTCGT CTGANGGCGA
  781 ANGCGAAGAN GCGGAANANG CCGCAGANCC GGCAGCANGC CGCGNNAANG AANGTCCGCT
  841 GGATTGAGGG CCGAANGGAC GTAGCAGAAN GANGTCCCGC GCAGAATCCA GGTGGCAACA
  901 CANGCNANCA GCCNTGGAAA GGNNGTCAGC TTCCCCNNNN ACACCNCNNN NNTGNCAGNG
961 CCNNACAGNC NANCCNNNNN NNNNCANCGG NNNANNNNNN NNNN
//
Primer: SV40pro_ori_F
Region: SV40pro_ori,Zeo,SV40p(A)
Sequence file: RDB12131_A2H6j.seq check
>04387_04387_A2H6_SV40pro_ori_F_H05_23_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNANG NCGCCTCTGC CTCTGANCTA TTCNNGAAGT AGTGAGGAGG
   61 CTTTTTTGGA GGCCTAGGCT TTTGCAAAAA GCTCCCGGGA GCTTGTATAT CCATTTTCGG
  121 ATCTGATCAG CACGTGTTGA CAATTAATCA TCGGCATAGT ATATCGGCAT AGTATAATAC
  181 GACAAGGTGA GGAACTAAAC CATGGCCAAG TTGACCAGTG CCGTTCCGGT GCTCACCGCG
  241 CGCGACGTCG CCGGAGCGGT CGAGTTCTGG ACCGACCGGC TCGGGTTCTC CCGGGACTTC
  301 GTGGAGGACG ACTTCGCCGG TGTGGTCCGG GACGACGTGA CCCTGTTCAT CAGCGCGGTC
  361 CAGGACCAGG TGGTGCCGGA CAACACCCTG GCCTGGGTGT GGGTGCGCGG CCTGGACGAG
  421 CTGTACGCCG AGTGGTCGGA GGTCGTGTCC ACGAACTTCC GGGACGCCTC CGGGCCGGCC
  481 ATGACCGAGA TCGGCGAGCA GCCGTGGGGG CGGGAGTTCG CCCTGCGCGA CCCGGCCGGC
  541 AACTGCGTGC ACTTCGTGGC CGAGGAGCAG GACTGACACG TGCTACGAGA TTTCGATTCC
  601 ACCGCCGCCT TCTATGAAAG GTTGGGCTTC GGAATCGTTT TCCGGGACGC CGGCTGGATG
  661 ATCCTCCAGC GCGGGGATCT CATGCTGGAG TTCTTCGCCC ACCCCAACTT GTTTATTGCA
  721 GCTTATAATG GTTACAAATA AAGCAATAGC ATCACAAATT TCACAAATAA AGCATTTTTT
  781 TCNCTGCATT CTAGTTGTGG TTTGTCCNAA CTCATCAATG TATCTNATCA TGTCTGTATA
  841 CCGTCGACCT CTAGCTAGAG CTTGGCGTAA TCATGGTCAT AGCNGTTNCC TGTGTGAAAT
  901 TGTTATCCGC TCNCNATTCC NCANNNCATA CNANNCGGNN NNATAAANTG NAAANCCTGG
  961 GGGTGNCTAA NGAGNGANCN NNNCACATTA NNNGCNNNGN NNNCNNNNNC NNNNTNNNNN
1021 NNNGNNNNCN NNNNNNNNN
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

user report Nishida, J., Epigenetic remodelling shapes inflammatory renal cancer and neutrophil-dependent metastasis. Nat. Cell Biol. 22 (4): 465-475 (2020). PMID 32203421.
user report Seki, R., Induced pluripotent stem cell-derived monocytic cell lines from a NOMID patient serve as a screening platform for modulating NLRP3 inflammasome activity. PLoS One 15 (8): e0237030 (2020). PMID 32810141.
user report Morikawa, M., The ALK-1/SMAD/ATOH8 axis attenuates hypoxic responses and protects against the development of pulmonary arterial hypertension. Sci. Signal. 12(607). pii: eaay4430 (2019). PMID 31719172.
user report Kambara, H., Gasdermin D Exerts Anti-inflammatory Effects by Promoting Neutrophil Death. Cell Rep. 22 (11): 2924-2936 (2018). PMID 29539421.
user report Yamamoto, E., Establishment and characterization of cell lines derived from complete hydatidiform mole. Int. J. Mol. Med. 40 (3): 614-622 (2017). PMID 28713902.
user report Suzuki, T., RUNX1 regulates site specificity of DNA demethylation by recruitment of DNA demethylation machineries in hematopoietic cells. Blood Adv. 1 (20): 1699-1711 (2017). PMID 29296817.
user report Imamura, Y., Generation of Large Numbers of Antigen-Expressing Human Dendritic Cells Using CD14-ML Technology. PLoS One. 2016 Apr 6;11(4):e0152384 (2016). PMID 27050553.
user report Inagawa, Y., A human cancer xenograft model utilizing normal pancreatic duct epithelial cells conditionally transformed with defined oncogenes. Carcinogenesis 35 (8): 1840-1846 (2014). PMID 24858378.
user report Moriyama, H., Tightly regulated and homogeneous transgene expression in human adipose-derived mesenchymal stem cells by lentivirus with tet-off system. PLoS One, 8 (6): e66274 (2013). PMID 23776652.
user report Yugawa, T., Noncanonical NOTCH signaling limits self-renewal of human epithelial and induced pluripotent stem cells through ROCK activation. Mol. Cell. Biol., 233 (22): 4434-4447 (2013). PMID 24019071.
user report Yoshita, M., Activation of mTOR by human T-cell leukemia virus type 1 Tax is important for the transformation of mouse T cells to interleukin-2-independent growth. Cancer Sci., 103 (2): 369-374 (2012). PMID 22010857.
user report Suzuki, T.,, Reconstruction of monocyte transcriptional regulatory network accompanies monocytic functions in human fibroblasts. PLoS One, 7 (3): e33474 (2012). PMID 22428058.
user report Makino, K., Discoidin domain receptor 2-microRNA 196a-mediated negative feedback against excess type I collagen expression is impaired in scleroderma dermal fibroblasts. J. Invest. Dermatol., 133 (1): 110-119 (2012). PMID 22832484.
user report Moriyama, M., Human adipose tissue-derived multilineage progenitor cells exposed to oxidative stress induce neurite outgrowth in PC12 cells through p38 MAPK signaling. BMC Cell Biol. 2012 Aug 7;13:21 (2012). PMID 22870983.
user report Kataoka, A., Involvement of vasodilator-stimulated phosphoprotein in UDP-induced microglial actin aggregation via PKC- and Rho-dependent pathways. Purinergic Signal, 7 (4): 403-411 (2011). PMID 21567128.
user report Yokomine K., The forkhead box M1 transcription factor as a candidate of target for anti-cancer immunotherapy. Int. J. Cancer., 126, 2153-2163 (2010). PMID 19688828.
user report Ito S., Expression of a chemokine BRAK/CXCL14 in oral floor carcinoma cells reduces the settlement rate of the cells and suppresses their proliferation in vivo. Biomed. Res., 31, 199-206 (2010). PMID 20622470.
user report Harao M., HLA-A2-restricted CTL epitopes of a novel lung cancer-associated cancer testis antigen, cell division cycle associated 1, can induce tumor-reactive CTL. Int. J. Cancer., 123, 2616-2625 (2008). PMID 18770861.
user report Imai K., Identification of a novel tumor-associated antigen, cadherin 3/P-cadherin, as a possible target for immunotherapy of pancreatic, gastric, and colorectal cancers. Clin. Cancer. Res., 14, 6487-6495 (2008). PMID 18927288.
user report Irie A., Protein kinase D2 contributes to either IL-2 promoter regulation or induction of cell death upon TCR stimulation depending on its activity in Jurkat cells. Int. Immunol., 18, 1737-1747 (2006). PMID 17077180.

2020.12.30

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