Resource data sheet
DNA Bank Top

CSII-EF-RfA (#RDB04387)

Backbone vector plasmid of lentivirus construct by Gateway(R) cloning with EF-1 alpha promoter.


Drawn by SnapGene® software
Sequence information
(Assembled from experimentally sequenced data)		 GenBank Flat File Format open
SnapGene file download
Publication Unpublished bioresource
Test sheet Data Sheet open 
 
Clone info. Backbone vector plasmid of lentivirus construct by Gateway(R) cloning with EF-1 alpha promoter.
Comment Commonly requested with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394).
Vector backbone CSII-EF-RfA (Plasmid)
Selectable markers Ampicillin, Chloramphenicol, ccdB (E. coli). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Growth conditions 37C, LB+Amp
Growth remarks Use DB3.1, ccdB Survival or equivalent to amplify this clone. 30 degC is fine.
Depositor|Developer Miyoshi, Hiroyuki |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Ordering instruction.[link] 
Terms and conditions for distribution The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer.
Remarks Remember that you will be working with samples containing infectious virus.
Use Survival2 (Invitrogen) or equivalent to amplify this clone.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「レンチウイルスベクターの提供申し込み」をご覧ください。
MTAに書く使用条件 本件研究材料は、非営利機関の非営利学術研究に限って提供する。本件研究材料を利用した研究結果等を発表する際は、本件研究材料が故三好浩之博士により開発されたことを明示する。
備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。
このクローンの増殖にはSurvival2 (Invitrogen)あるいは同等の宿主を使用してください。

Catalog # Resource name Shipping form Fee
RDB04387 CSII-EF-RfA DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Ordering instruction of plasmids [in Japanese] [in English]

How to cite this biological resource

Materials & Methods section:

The CSII-EF-RfA was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB04387).

Reference section:

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

user_report Yamamoto, S., Bovine respiratory syncytial virus enhances the attachment of Trueperella pyogenes to cells. J. Vet. Med. Sci. 86 (10): 1068-1075 (2024). PMID 39111845. [PubMed] [Article] [RRC of NBRP]
user_report Kawamura, Y., Cellular senescence induction leads to progressive cell death via the INK4a-RB pathway in naked mole-rats. EMBO J. 2023 Aug 15;42(16):e111133 PMID 37431790. [PubMed] [Article] [RRC of NBRP]
user_report Nishitani-Isa, M., Trapping of CDC42 C-terminal variants in the Golgi drives pyrin inflammasome hyperactivation. J. Exp. Med. 219 (6): e20211889 (2022). PMID 35482294. [PubMed] [Article] [RRC of NBRP]
user_report Takahashi, K., TGF-β generates a population of cancer cells residing in G1 phase with high motility and metastatic potential via KRTAP2-3. Cell Rep. 40 (13): 111411 (2022). PMID 36170816. [PubMed] [Article] [RRC of NBRP]
user_report Ozawa, T., Systemic administration of monovalent follistatin-like 3-Fc-fusion protein increases muscle mass in mice. iScience 24 (5): 102488 (2021). PMID 34113826. [PubMed] [Article] [RRC of NBRP]
user_report Ozawa, T., Preparation of monovalent follistatin-like 3-Fc-fusion protein and evaluation of its effects on muscle mass in mice. STAR Protoc. 2 (4): 100839 (2021). PMID 34585166. [PubMed] [Article] [RRC of NBRP]
user_report Nishida, J., Epigenetic remodelling shapes inflammatory renal cancer and neutrophil-dependent metastasis. Nat. Cell Biol. 22 (4): 465-475 (2020). PMID 32203421. [PubMed] [Article] [RRC of NBRP]
user_report Seki, R., Induced pluripotent stem cell-derived monocytic cell lines from a NOMID patient serve as a screening platform for modulating NLRP3 inflammasome activity. PLoS One 15 (8): e0237030 (2020). PMID 32810141. [PubMed] [Article] [RRC of NBRP]
user_report Morikawa, M., The ALK-1/SMAD/ATOH8 axis attenuates hypoxic responses and protects against the development of pulmonary arterial hypertension. Sci. Signal. 12(607). pii: eaay4430 (2019). PMID 31719172. [PubMed] [Article] [RRC of NBRP]
user_report Kambara, H., Gasdermin D Exerts Anti-inflammatory Effects by Promoting Neutrophil Death. Cell Rep. 22 (11): 2924-2936 (2018). PMID 29539421. [PubMed] [Article] [RRC of NBRP]
user_report Kubota, S.I., Whole-Body Profiling of Cancer Metastasis with Single-Cell Resolution. Cell Rep. 20 (1): 236-250 (2017). PMID 28683317. [PubMed] [Article] [RRC of NBRP]
user_report Yamamoto, E., Establishment and characterization of cell lines derived from complete hydatidiform mole. Int. J. Mol. Med. 40 (3): 614-622 (2017). PMID 28713902. [PubMed] [Article] [RRC of NBRP]
user_report Suzuki, T., RUNX1 regulates site specificity of DNA demethylation by recruitment of DNA demethylation machineries in hematopoietic cells. Blood Adv. 1 (20): 1699-1711 (2017). PMID 29296817. [PubMed] [Article] [RRC of NBRP]
user_report Imamura, Y., Generation of Large Numbers of Antigen-Expressing Human Dendritic Cells Using CD14-ML Technology. PLoS One. 2016 Apr 6;11(4):e0152384 (2016). PMID 27050553. [PubMed] [Article] [RRC of NBRP]
user_report Inagawa, Y., A human cancer xenograft model utilizing normal pancreatic duct epithelial cells conditionally transformed with defined oncogenes. Carcinogenesis 35 (8): 1840-1846 (2014). PMID 24858378. [PubMed] [Article] [RRC of NBRP]
user_report Moriyama, H., Tightly regulated and homogeneous transgene expression in human adipose-derived mesenchymal stem cells by lentivirus with tet-off system. PLoS One, 8 (6): e66274 (2013). PMID 23776652. [PubMed] [Article] [RRC of NBRP]
user_report Yugawa, T., Noncanonical NOTCH signaling limits self-renewal of human epithelial and induced pluripotent stem cells through ROCK activation. Mol. Cell. Biol., 233 (22): 4434-4447 (2013). PMID 24019071. [PubMed] [Article] [RRC of NBRP]
user_report Yoshita, M., Activation of mTOR by human T-cell leukemia virus type 1 Tax is important for the transformation of mouse T cells to interleukin-2-independent growth. Cancer Sci., 103 (2): 369-374 (2012). PMID 22010857. [PubMed] [Article] [RRC of NBRP]
user_report Suzuki, T.,, Reconstruction of monocyte transcriptional regulatory network accompanies monocytic functions in human fibroblasts. PLoS One, 7 (3): e33474 (2012). PMID 22428058. [PubMed] [Article] [RRC of NBRP]
user_report Makino, K., Discoidin domain receptor 2-microRNA 196a-mediated negative feedback against excess type I collagen expression is impaired in scleroderma dermal fibroblasts. J. Invest. Dermatol., 133 (1): 110-119 (2012). PMID 22832484. [PubMed] [Article] [RRC of NBRP]
user_report Moriyama, M., Human adipose tissue-derived multilineage progenitor cells exposed to oxidative stress induce neurite outgrowth in PC12 cells through p38 MAPK signaling. BMC Cell Biol. 2012 Aug 7;13:21 (2012). PMID 22870983. [PubMed] [Article] [RRC of NBRP]
user_report Kataoka, A., Involvement of vasodilator-stimulated phosphoprotein in UDP-induced microglial actin aggregation via PKC- and Rho-dependent pathways. Purinerg. Signal, 7 (4): 403-411 (2011). PMID 21567128. [PubMed] [Article] [RRC of NBRP]
user_report Yokomine K., The forkhead box M1 transcription factor as a candidate of target for anti-cancer immunotherapy. Int. J. Cancer., 126, 2153-2163 (2010). PMID 19688828. [PubMed] [Article] [RRC of NBRP]
user_report Ito S., Expression of a chemokine BRAK/CXCL14 in oral floor carcinoma cells reduces the settlement rate of the cells and suppresses their proliferation in vivo. Biomed. Res., 31, 199-206 (2010). PMID 20622470. [PubMed] [Article] [RRC of NBRP]
user_report Harao M., HLA-A2-restricted CTL epitopes of a novel lung cancer-associated cancer testis antigen, cell division cycle associated 1, can induce tumor-reactive CTL. Int. J. Cancer., 123, 2616-2625 (2008). PMID 18770861. [PubMed] [Article] [RRC of NBRP]
user_report Imai K., Identification of a novel tumor-associated antigen, cadherin 3/P-cadherin, as a possible target for immunotherapy of pancreatic, gastric, and colorectal cancers. Clin. Cancer. Res., 14, 6487-6495 (2008). PMID 18927288. [PubMed] [Article] [RRC of NBRP]
user_report Irie A., Protein kinase D2 contributes to either IL-2 promoter regulation or induction of cell death upon TCR stimulation depending on its activity in Jurkat cells. Int. Immunol., 18, 1737-1747 (2006). PMID 17077180. [PubMed] [Article] [RRC of NBRP]
user_report Tanabe, R., PRRX1 induced by BMP signaling decreases tumorigenesis by epigenetically regulating glioma-initiating cell properties via DNA methyltransferase 3A. Mol. Oncol. 16 (1): 269-288 (2022). PMID 34214250. [PubMed] [Article] [RRC of NBRP]