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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

CSII-EF-RfA

Backbone vector plasmid of lentivirus construct by Gateway(R) cloning with EF-1 alpha promoter.

Catalog number RDB04387
Resource name CSII-EF-RfA
Clone info. Backbone vector plasmid of lentivirus construct by Gateway(R) cloning with EF-1 alpha promoter.
Comment Commonly requested with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Vector backbone CSII-EF-RfA (Plasmid)
Selectable markers Please note that Zeo resistance marker is not included in the lentivirus produced from this vector. E. coli: Amp^r, Cm^r, ccdB.
Growth remarks Use DB3.1, ccdB Survival or equivalent to amplify this clone. 30 degC is fine.
Depositor|Developer Miyoshi, Hiroyuki |
Other clones in our bank
External Database
            Reference sequence
              
            Lentiviral plasmid list. [link]

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer.
            Ordering Please visit Ordering instruction.[link] 
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            Material Transfer Agreement (MTA, for use for not-for-profit academic purpose)[Word]
            Remarks Remember that you will be working with samples containing infectious virus.
            Use Survival2 (Invitrogen) or equivalent to amplify this clone.
            提供案内 (日本国内) [open/close]

            提供条件 本件研究材料は、非営利機関の非営利学術研究に限って提供する。本件研究材料を利用した研究結果等を発表する際は、本件研究材料が故三好浩之博士により開発されたことを明示する。
            提供依頼 手続きの詳細は、「レンチウイルスベクターの提供申し込み」をご覧ください。
            提供依頼書 [Word]
            提供同意書 (非営利機関による非営利学術研究用)[Word]
            遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。
            備考 【追加提供依頼書】受入れ確認書(書式J)
            このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。
            このクローンの増殖にはSurvival2 (Invitrogen)あるいは同等の宿主を使用してください。

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB04387 CSII-EF-RfA DNA solution

            Ordering instruction of plasmids [in Japanese] [in English]
            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Electronic file

            Sequence (full) RDB04387zzk02.seq provided by the depositor
            Remarks, protocol and/or map (pdf) RDB04387.pdf

            Original reference

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Lentivirus Vector (English text)
            Featured content Lentivirus Vector (Japanese text)

            Sequence information

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB12131_A2H6p1-3.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: EF1a-C_F
            Region: EF-1alpha pro,RfA(attR1,lacUV5 pro,CmR)
            Sequence file: RDB12131_A2H6e.seq check
            >04387_04387_A2H6_EF1a-C_F_C05_08_ABI24.ab1
                1 NNNNNNNNNN NNNNTTTTGA NTTTGGNNCT TGGTTNNTTC TCNAGCCTCA GACAGTGGTT
               61 CAAAGTTTTT TTCTTCCATT TCAGGTGTCG TGAACACGCT ACCGGTCTCG AGAATTATCA
              121 CAAGTTTGTA CAAAAAAGCT GAACGAGAAA CGTAAAATGA TATAAATATC AATATATTAA
              181 ATTAGATTTT GCATAAAAAA CAGACTACAT AATACTGTAA AACACAACAT ATCCAGTCAC
              241 TATGGCGGCC GCATTAGGCA CCCCAGGCTT TACACTTTAT GCTTCCGGCT CGTATAATGT
              301 GTGGATTTTG AGTTAGGATC CGTCGAGATT TTCAGGAGCT AAGGAAGCTA AAATGGAGAA
              361 AAAAATCACT GGATATACCA CCGTTGATAT ATCCCAATGG CATCGTAAAG AACATTTTGA
              421 GGCATTTCAG TCAGTTGCTC AATGTACCTA TAACCAGACC GTTCAGCTGG ATATTACGGC
              481 CTTTTTAAAG ACCGTAAAGA AAAATAAGCA CAAGTTTTAT CCGGCCTTTA TTCACATTCT
              541 TGCCCGCCTG ATGAATGCTC ATCCGGAATT CCGTATGGCA ATGAAAGACG GTGAGCTGGT
              601 GATATGGGAT AGTGTTCACC CTTGTTACAC CGTTTTCCAT GAGCAAACTG AAACGTTTTC
              661 ATCGCTCTGG AGTGAATACC ACGACGATTT CCGGCAGTTT CTACACATAT ATTCGCAAGA
              721 TGTGGCGTGT TACGGTGAAA ACCTGNCCTA TTTCCCTAAA GGGTTTATTG AGAATATGTT
              781 TTTCGTCTCA GCCAATCCCT GGGTGAGTTT CACCAGTTTT GATTTAAACG TGGCCNATAT
              841 GGACAACTTC TTCGCCCCCG TTTTCACCAN GGGCNAATAT TATACGCNAG GCGNNNANGT
              901 GCTGANGNCG CTGGCNANTC NNNNCATCAT GCCNTTNGNG ATGGNNTCCA NGTCGGCNNA
            961 ATGCTTNNTG NNNTACNANN NNANNGCNNT GANNNNNNNN NN
            //
            Primer: ccdB-C_F
            Region: RfA(ccdB,attR2),PRE
            Sequence file: RDB12131_A2H6f.seq check
            >04387_04387_A2H6_ccdB-C_F_D05_11_ABI24.ab1
                1 NNNNNNNNNN NNNNNTCGGG GNNGAANCTG GCGCATGATG ACCNCCGATA TGGCCAGTGT
               61 GCCGGTCTCC GTTATCGGGG AAGAAGTGGC TGATCTCAGC CACCGCGAAA ATGACATCAA
              121 AAACGCCATT AACCTGATGT TCTGGGGAAT ATAAATGTCA GGCTCCCTTA TACACAGCCA
              181 GTCTGCAGGT CGACCATAGT GACTGGATAT GTTGTGTTTT ACAGTATTAT GTAGTCTGTT
              241 TTTTATGCAA AATCTAATTT AATATATTGA TATTTATATC ATTTTACGTT TCTCGTTCAG
              301 CTTTCTTGTA CAAAGTGGTG ATAATTCTGC AGCGGCCGCG GATCCTCTAG AGTTAACATC
              361 GAGGGATCAA GCTTATCGAT AATCAACCTC TGGATTACAA AATTTGTGAA AGATTGACTG
              421 GTATTCTTAA CTATGTTGCT CCTTTTACGC TATGTGGATA CGCTGCTTTA ATGCCTTTGT
              481 ATCATGCTAT TGCTTCCCGT ATGGCTTTCA TTTTCTCCTC CTTGTATAAA TCCTGGTTGC
              541 TGTCTCTTTA TGAGGAGTTG TGGCCCGTTG TCAGGCAACG TGGCGTGGTG TGCACTGTGT
              601 TTGCTGACGC AACCCCCACT GGTTGGGGCA TTGCCACCAC CTGTCAGCTC CTTTCCGGGA
              661 CTTTCGCTTT CCCCCTCCCT ATTGCCACGG CGGAACTCAT CGCCGCCTGC CTTGCCCGCT
              721 GCTGGACAGG GGCTCGGCTG TTGGGCACTG ACAATTCCGT GGTGTTGTCG GGGAANCTGA
              781 CGTCCTTTCC NTGGCTGCTC GCCTGTGTTG CCACCTGGAT TCTGCGCGGG ANGTCCTTCT
              841 GCTACGTCCC TTCGGCCNTC NATCCAGCGG ANCTTNCTTN CCGCGGCCTG CTGNNGGNNN
              901 TGCGGNNNCT TNNNNGNCTT CNCCTTCGCC CTCAGACGAG TCGGATCNNC NNTTGGGCCN
            961 NCNCCCCGCN TCNATANNNN CNNNANNNAN AAAAANNTNN
            //
            Primer: CMV_Forward
            Region: CMVpro,5'LTR,HIV-1psi
            Sequence file: RDB12131_A2H6g.seq check
            >04387_04387_A2H6_CMV_Forward_E05_14_ABI24.ab1
                1 NNNNNNNNNN NNNNNTGNNG CNNNGGGCGG TAGGCGTGTA CGGTGGGAGG TCTATATAAG
               61 CAGCGCGTTT TGCCTGTACT GGGTCTCTCT GGTTAGACCA GATCTGAGCC TGGGAGCTCT
              121 CTGGCTAACT AGGGAACCCA CTGCTTAAGC CTCAATAAAG CTTGCCTTGA GTGCTTCAAG
              181 TAGTGTGTGC CCGTCTGTTG TGTGACTCTG GTAACTAGAG ATCCCTCAGA CCCTTTTAGT
              241 CAGTGTGGAA AATCTCTAGC AGTGGCGCCC GAACAGGGAC TTGAAAGCGA AAGGGAAACC
              301 AGAGGAGCTC TCTCGACGCA GGACTCGGCT TGCTGAAGCG CGCACGGCAA GAGGCGAGGG
              361 GCGGCGACTG GTGAGTACGC CAAAAATTTT GACTAGCGGA GGCTAGAAGG AGAGAGATGG
              421 GTGCGAGAGC GTCAGTATTA AGCGGGGGAG AATTAGATCG CGATGGGAAA AAATTCGGTT
              481 AAGGCCAGGG GGAAAGAAAA AATATAAATT AAAACATATA GTATGGGCAA GCAGGGAGCT
              541 AGAACGATTC GCAGTTAATC CTGGCCTGTT AGAAACATCA GAAGGCTGTA GACAAATACT
              601 GGGACAGCTA CAACCATCCC TTCAGACAGG ATCAGAAGAA CTTAGATCAT TATATAATAC
              661 AGTAGCAACC CTCTATTGTG TGCATCAAAG GATAGAGATA AAAGACACCA AGGAAGCTTT
              721 AGACAAGATA GAGGAAGAGC AAAACAAAAG TAAGANCACC GCACAGCAAG GGCCGCTGAT
              781 CTTCAGACNT GGAGGANGNN NTATGANGGA NAATTGGAGA AGTGAATTAT ATAAATATAA
              841 AGTAGTAAAA ATTGAACCNT TANGAGTAGC ACNNNCNNAN GNNNNGAGAA NANNGGTGCA
              901 GANAGAAAAA AAGANCAGNG GGAATAGGAN NTTTGTNNCT NNGGGNNCNN GGNNNCAGCN
            961 NNAANCNCTA NNGGNNNNNN NNNN
            //
            Primer: cPPT/CTS-E_R
            Region: cPPT_CTS,RRE
            Sequence file: RDB12131_A2H6h.seq check
            >04387_04387_A2H6_cPPT_CTS-E_R_F05_17_ABI24.ab1
                1 NNNNNNNNNT NGNTTTTNNN NNCTTTAGTT TGTATGTCTG TTGCTATTAT GTCTACTATT
               61 CTTTCCCCTG CACTGTACCC CCCAATCCCC CCTTTTCTTT TAAAATTGTG GATGAATACT
              121 GCCATTTGTA GATCCGTTCA CTAATCGAAT GGATCTGTCT CTGTCTCTCT CTCCACCTTC
              181 TTCTTCTATT CCTTCGGGCC TGTCGGGTCC CCTCGGGGTT GGGAGGTGGG TCTGAAACGA
              241 TAATGGTGAA TATCCCTGCC TAACTCTATT CACTATAGAA AGTACAGCAA AAACTATTCT
              301 TAAACCTACC AAGCCTCCTA CTATCATTAT GAATAATTTT ATATACCACA GCCAATTTGT
              361 TATGTTAAAC CAATTCCACA AACTTGCCCA TTTATCTAAT TCCAATAATT CTTGTTCATT
              421 CTTTTCTTGC TGGTTTTGCG ATTCTTCAAT TAAGGAGTGT ATTAAGCTTG TGTAATTGTT
              481 AATTTCTCTG TCCCACTCCA TCCAGGTCGT GTGATTCCAA ATCTGTTCCA GAGATTTATT
              541 ACTCCAACTA GCATTCCAAG GCACAGCAGT GGTGCAAATG AGTTTTCCAG AGCAACCCCA
              601 AATCCCCAGG AGCTGTTGAT CCTTTAGGTA TCTTTCCACA GCCAGGATTC TTGCCTGGAG
              661 CTGCTTGATG CCCCAGACTG TGAGTTGCAA CAGATGCTGT TGCGCCTCAA TAGCCCTCAG
              721 CAAATTGTTC TGCTGCTGCA CTATACCAGA CAATAATTGT CTGGCCTGTA NCGTCAGCGT
              781 CATTGACGCT GCGCNCATAG TGCTTCCTGC TGCTCNNNAG AACCCAAGGA ACAAAGCTCC
              841 TATTCCCACT GCTCTTTTTT TCTCTCTGCA CCACTCTTCT CTTTGCCTNG GNGGGTGCTA
              901 CTCCNAANGG TTCAATTTTN NCTACTTTAT ATTTATATAN NTCACTTCTN NNATTGTCCN
            961 NCATANCNNC NNNNNCANNN NNNNNNN
            //
            Primer: bGH-B_R
            Region: 3'LTR,PRE
            Sequence file: RDB12131_A2H6i.seq check
            >04387_04387_A2H6_bGH-B_R_G05_20_ABI24.ab1
                1 NNNNNNNNNN NNNNTNNNNN GCGGGTNNNN NNGGCCCTGC TAGAGANTTT CCNCACTGAC
               61 TAAAAGGGTC TGAGGGATCT CTAGTTACCA GAGTCACACA ACAGACGGGC ACACACTACT
              121 TGAAGCACTC AAGGCAAGCT TTATTGAGGC TTAAGCAGTG GGTTCCCTAG TTAGCCAGAG
              181 AGCTCCCAGG CTCAGATCTG GTCTAACCAG AGAGACCCAG TACAGTCCGG ATGCAGCTCT
              241 CGGGCCATGT GATGAAATGC TAGGCGGCTG TCAAACCTCC ACTCTAATAC TTCTCTCTCC
              301 GGGTCATCCA TCCCATGCAG GCTCACAGGG TGTAACAAGC GGGTGTTCTC TCCTTCATTG
              361 GCTTCTTCTA CCTTCTCTTG CTCAACTGGT ACTAGCTTGT AGCACCATCC AAAGGTCAGT
              421 GGATATCTGA TCCCTGGCCC TGGTGTGTAG TTCTGCCAAT CAGGGAAGTA GCCTTGTGTG
              481 TGGTAGATCC ACAGATCAAG GATATCTTGT CTTCGTTGGG AGTGAATTAG CCCTTCCAGT
              541 CCCCCCTTTT CTTTTAAAAA GTGGCTAAGA TCTACAGCTG CCTTGTAAGT CATTGGTCTT
              601 AAAGGTACCT GAGGTGTGAC TGGAAAACCC ACCTCCTCCT CCTCTTGTGC TTCTAGCCAG
              661 GCACAATCAG CATTGGTAGC TGCTGTATTG CTACTTGTGA TTGCTCCATG TTTTTCTAGG
              721 TCTCGACGGT ATCGATGCGG GGAGGCGGCC CAAAGGGAGA TCCGACTCGT CTGANGGCGA
              781 ANGCGAAGAN GCGGAANANG CCGCAGANCC GGCAGCANGC CGCGNNAANG AANGTCCGCT
              841 GGATTGAGGG CCGAANGGAC GTAGCAGAAN GANGTCCCGC GCAGAATCCA GGTGGCAACA
              901 CANGCNANCA GCCNTGGAAA GGNNGTCAGC TTCCCCNNNN ACACCNCNNN NNTGNCAGNG
            961 CCNNACAGNC NANCCNNNNN NNNNCANCGG NNNANNNNNN NNNN
            //
            Primer: SV40pro_ori_F
            Region: SV40pro_ori,Zeo,SV40p(A)
            Sequence file: RDB12131_A2H6j.seq check
            >04387_04387_A2H6_SV40pro_ori_F_H05_23_ABI24.ab1
                1 NNNNNNNNNN NNNNNNNANG NCGCCTCTGC CTCTGANCTA TTCNNGAAGT AGTGAGGAGG
               61 CTTTTTTGGA GGCCTAGGCT TTTGCAAAAA GCTCCCGGGA GCTTGTATAT CCATTTTCGG
              121 ATCTGATCAG CACGTGTTGA CAATTAATCA TCGGCATAGT ATATCGGCAT AGTATAATAC
              181 GACAAGGTGA GGAACTAAAC CATGGCCAAG TTGACCAGTG CCGTTCCGGT GCTCACCGCG
              241 CGCGACGTCG CCGGAGCGGT CGAGTTCTGG ACCGACCGGC TCGGGTTCTC CCGGGACTTC
              301 GTGGAGGACG ACTTCGCCGG TGTGGTCCGG GACGACGTGA CCCTGTTCAT CAGCGCGGTC
              361 CAGGACCAGG TGGTGCCGGA CAACACCCTG GCCTGGGTGT GGGTGCGCGG CCTGGACGAG
              421 CTGTACGCCG AGTGGTCGGA GGTCGTGTCC ACGAACTTCC GGGACGCCTC CGGGCCGGCC
              481 ATGACCGAGA TCGGCGAGCA GCCGTGGGGG CGGGAGTTCG CCCTGCGCGA CCCGGCCGGC
              541 AACTGCGTGC ACTTCGTGGC CGAGGAGCAG GACTGACACG TGCTACGAGA TTTCGATTCC
              601 ACCGCCGCCT TCTATGAAAG GTTGGGCTTC GGAATCGTTT TCCGGGACGC CGGCTGGATG
              661 ATCCTCCAGC GCGGGGATCT CATGCTGGAG TTCTTCGCCC ACCCCAACTT GTTTATTGCA
              721 GCTTATAATG GTTACAAATA AAGCAATAGC ATCACAAATT TCACAAATAA AGCATTTTTT
              781 TCNCTGCATT CTAGTTGTGG TTTGTCCNAA CTCATCAATG TATCTNATCA TGTCTGTATA
              841 CCGTCGACCT CTAGCTAGAG CTTGGCGTAA TCATGGTCAT AGCNGTTNCC TGTGTGAAAT
              901 TGTTATCCGC TCNCNATTCC NCANNNCATA CNANNCGGNN NNATAAANTG NAAANCCTGG
              961 GGGTGNCTAA NGAGNGANCN NNNCACATTA NNNGCNNNGN NNNCNNNNNC NNNNTNNNNN
            1021 NNNGNNNNCN NNNNNNNNN
            //

            Please visit Sequencing and PCR primers for primer information.

            References

            Original, user report and related articles

            user_report Ozawa, T., Systemic administration of monovalent follistatin-like 3-Fc-fusion protein increases muscle mass in mice. iScience 24 (5): 102488 (2021). PMID 34113826.
            user_report Ozawa, T., Preparation of monovalent follistatin-like 3-Fc-fusion protein and evaluation of its effects on muscle mass in mice. STAR Protoc. 2 (4): 100839 (2021). PMID 34585166.
            user_report Nishida, J., Epigenetic remodelling shapes inflammatory renal cancer and neutrophil-dependent metastasis. Nat. Cell Biol. 22 (4): 465-475 (2020). PMID 32203421.
            user_report Seki, R., Induced pluripotent stem cell-derived monocytic cell lines from a NOMID patient serve as a screening platform for modulating NLRP3 inflammasome activity. PLoS One 15 (8): e0237030 (2020). PMID 32810141.
            user_report Morikawa, M., The ALK-1/SMAD/ATOH8 axis attenuates hypoxic responses and protects against the development of pulmonary arterial hypertension. Sci. Signal. 12(607). pii: eaay4430 (2019). PMID 31719172.
            user_report Kambara, H., Gasdermin D Exerts Anti-inflammatory Effects by Promoting Neutrophil Death. Cell Rep. 22 (11): 2924-2936 (2018). PMID 29539421.
            user_report Kubota, S.I., Whole-Body Profiling of Cancer Metastasis with Single-Cell Resolution. Cell Rep. 20 (1): 236-250 (2017). PMID 28683317.
            user_report Yamamoto, E., Establishment and characterization of cell lines derived from complete hydatidiform mole. Int. J. Mol. Med. 40 (3): 614-622 (2017). PMID 28713902.
            user_report Suzuki, T., RUNX1 regulates site specificity of DNA demethylation by recruitment of DNA demethylation machineries in hematopoietic cells. Blood Adv. 1 (20): 1699-1711 (2017). PMID 29296817.
            user_report Imamura, Y., Generation of Large Numbers of Antigen-Expressing Human Dendritic Cells Using CD14-ML Technology. PLoS One. 2016 Apr 6;11(4):e0152384 (2016). PMID 27050553.
            user_report Inagawa, Y., A human cancer xenograft model utilizing normal pancreatic duct epithelial cells conditionally transformed with defined oncogenes. Carcinogenesis 35 (8): 1840-1846 (2014). PMID 24858378.
            user_report Moriyama, H., Tightly regulated and homogeneous transgene expression in human adipose-derived mesenchymal stem cells by lentivirus with tet-off system. PLoS One, 8 (6): e66274 (2013). PMID 23776652.
            user_report Yugawa, T., Noncanonical NOTCH signaling limits self-renewal of human epithelial and induced pluripotent stem cells through ROCK activation. Mol. Cell. Biol., 233 (22): 4434-4447 (2013). PMID 24019071.
            user_report Yoshita, M., Activation of mTOR by human T-cell leukemia virus type 1 Tax is important for the transformation of mouse T cells to interleukin-2-independent growth. Cancer Sci., 103 (2): 369-374 (2012). PMID 22010857.
            user_report Suzuki, T.,, Reconstruction of monocyte transcriptional regulatory network accompanies monocytic functions in human fibroblasts. PLoS One, 7 (3): e33474 (2012). PMID 22428058.
            user_report Makino, K., Discoidin domain receptor 2-microRNA 196a-mediated negative feedback against excess type I collagen expression is impaired in scleroderma dermal fibroblasts. J. Invest. Dermatol., 133 (1): 110-119 (2012). PMID 22832484.
            user_report Moriyama, M., Human adipose tissue-derived multilineage progenitor cells exposed to oxidative stress induce neurite outgrowth in PC12 cells through p38 MAPK signaling. BMC Cell Biol. 2012 Aug 7;13:21 (2012). PMID 22870983.
            user_report Kataoka, A., Involvement of vasodilator-stimulated phosphoprotein in UDP-induced microglial actin aggregation via PKC- and Rho-dependent pathways. Purinergic Signal, 7 (4): 403-411 (2011). PMID 21567128.
            user_report Yokomine K., The forkhead box M1 transcription factor as a candidate of target for anti-cancer immunotherapy. Int. J. Cancer., 126, 2153-2163 (2010). PMID 19688828.
            user_report Ito S., Expression of a chemokine BRAK/CXCL14 in oral floor carcinoma cells reduces the settlement rate of the cells and suppresses their proliferation in vivo. Biomed. Res., 31, 199-206 (2010). PMID 20622470.
            user_report Harao M., HLA-A2-restricted CTL epitopes of a novel lung cancer-associated cancer testis antigen, cell division cycle associated 1, can induce tumor-reactive CTL. Int. J. Cancer., 123, 2616-2625 (2008). PMID 18770861.
            user_report Imai K., Identification of a novel tumor-associated antigen, cadherin 3/P-cadherin, as a possible target for immunotherapy of pancreatic, gastric, and colorectal cancers. Clin. Cancer. Res., 14, 6487-6495 (2008). PMID 18927288.
            user_report Irie A., Protein kinase D2 contributes to either IL-2 promoter regulation or induction of cell death upon TCR stimulation depending on its activity in Jurkat cells. Int. Immunol., 18, 1737-1747 (2006). PMID 17077180.

            2022.05.18

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