Resource data sheet
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CSII-EF-RfA

Shuttle vector to generate recombinant Lentiviral vector

Catalog number RDB04387
Resource name CSII-EF-RfA
Clone info. Shuttle vector to generate recombinant Lentiviral vector
Use DB3.1 (Invitrogen) or equivalent to amplify this clone. Commonly requested with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394).
Vector backbone Plasmid
Selectable markers Amp^r
Gene/insert name Vector
Originator Miyoshi, Hiroyuki |

Distribution information

CLICK here for plasmid list and map. [link]
Forms for plasmid request [in Japanese] [in English]
Terms and conditions for distribution The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose.
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Remarks ((Remarks)) Remember that you will be working with samples containing infectious virus.
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Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB12131_A5Ccp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxEF_F1 RDB12131_A5Cca.seq
>04387_12131_A5Cc_pAxEF_F1_D01_10.ab1
    1 ATGTTTTCTT CATTTCAGGT GTCGTGACAC GCTACCGGTC TCGAGAATTA TCACAAGTTT
   61 GTACAAAAAA GCTGAACGAG AAACGTAAAA TGATATAAAT ATCAATATAT TAAATTAGAT
  121 TTTGCATAAA AAACAGACTA CATAATACTG TAAAACACAA CATATCCAGT CACTATGGCG
  181 GCCGCATTAG GCACCCCAGG CTTTACACTT TATGCTTCCG GCTCGTATAA TGTGTGGATT
  241 TTGAGTTAGG ATCCGTCGAG ATTTTCAGGA GCTAAGGAAG CTAAAATGGA GAAAAAAATC
  301 ACTGGATATA CCACCGTTGA TATATCCCAA TGGCATCGTA AAGAACATTT TGAGGCATTT
  361 CAGTCAGTTG CTCAATGTAC CTATAACCAG ACCGTTCAGC TGGATATTAC GGCCTTTTTA
  421 AAGACCGTAA AGAAAAATAA GCACAAGTTT TATCCGGCCT TTATTCACAT TCTTGCCCGC
  481 CTGATGAATG CTCATCCGGA ATTCCGTATG GCAATGAAAG ACGGTGAGCT GGTGATATGG
  541 GATAGTGTTC ACCCTTGTTA CACCGTTTTC CATGAGCAAA CTGAAACGTT TTCATCGCTC
  601 TGGAGTGAAT ACCACGACGA TTTCCGGCAG TTTCTACACA TATATTCGCA AGATGTGGCG
  661 TGTTACGGTG AAAACCTGGC CTATTTCCCT AAAGGGTTTA TTGAGAATAT GTTTTTCGTC
  721 TCAGCCAATC CCTGGGTGAG TTTCACCAGT TTTGATTTAA ACGTGGCCAA TATGGACAAC
  781 TTCTTCGCCC CCGTTTTCAC CATGGGCAAA TATTATACGC AAGGCGACAA GGTGCTGATG
  841 CCGCTGGCGA TTCAGGTTCA TCATGCCGTT TGTGATGGCT TCCATGTCGG CAGAATGCTT
  901 AATGAATTAC AACAGTACTG CGATGAGTGG CAGGGCGGGG CGTAAACGCG TGGATCCGGC
  961 TTACTAAAAG CCAGATAACA GTATGCGTAT TTGCGCGCTG ATTTTTGCGG TATAAGAATA
 1021 TATACTGATA TGTATACCCG AAGTATGTCA AAAAGAGGTA TGCCTATGAA GCAGCGTATT
 1081 AACAGTGACA GCTGACAGCG ACCAGGCCTA TCCAGGCTTG CTCAAGGTCA ATATTAAGTG
1141 CATGGTTCCA TTAATT
//
Primer: ccdB-C_F RDB12131_A5Ccb.seq
>04387_12131_A5Cc_ccdB-C_F_E01_13.ab1
    1 GTACGGGCGG GTGCATATCG GGGATGAAGC TGGCGCATGA TGACCACCGA TATGGCCAGT
   61 GTGCCGGTCT CCGTTATCGG GGAAGAAGTG GCTGATCTCA GCCACCGCGA AAATGACATC
  121 AAAAACGCCA TTAACCTGAT GTTCTGGGGA ATATAAATGT CAGGCTCCCT TATACACAGC
  181 CAGTCTGCAG GTCGACCATA GTGACTGGAT ATGTTGTGTT TTACAGTATT ATGTAGTCTG
  241 TTTTTTATGC AAAATCTAAT TTAATATATT GATATTTATA TCATTTTACG TTTCTCGTTC
  301 AGCTTTCTTG TACAAAGTGG TGATAATTCT GCAGCGGCCG CGGATCCTCT AGAGTTAACA
  361 TCGAGGGATC AAGCTTATCG ATAATCAACC TCTGGATTAC AAAATTTGTG AAAGATTGAC
  421 TGGTATTCTT AACTATGTTG CTCCTTTTAC GCTATGTGGA TACGCTGCTT TAATGCCTTT
  481 GTATCATGCT ATTGCTTCCC GTATGGCTTT CATTTTCTCC TCCTTGTATA AATCCTGGTT
  541 GCTGTCTCTT TATGAGGAGT TGTGGCCCGT TGTCAGGCAA CGTGGCGTGG TGTGCACTGT
  601 GTTTGCTGAC GCAACCCCCA CTGGTTGGGG CATTGCCACC ACCTGTCAGC TCCTTTCCGG
  661 GACTTTCGCT TTCCCCCTCC CTATTGCCAC GGCGGAACTC ATCGCCGCCT GCCTTGCCCG
  721 CTGCTGGACA GGGGCTCGGC TGTTGGGCAC TGACAATTCC GTGGTGTTGT CGGGGAAGCT
  781 GACGTCCTTT CCATGGCTGC TCGCCTGTGT TGCCACCTGG ATTCTGCGCG GGACGTCCTT
  841 CTGCTACGTC CCTTCGGCCC TCAATCCAGC GGACCTTCCT TCCCGCGGCC TGCTGCCGGC
  901 TCTGCGGGCC TCTTCCGCGT CTTCGCCTTC GCCCTCAGAC GAGTCGGATC TCCCTTTGGG
  961 CCGCCTTCCC CCGCATCGAT ACCGTCGAGA CCTAGAAAAA CATGGAGCAA TCACAGGTAG
 1021 CATACAGCAG CTACCATGCT GATGTGCTTG CTAGAGCACA GAGAAGAGAG TTGTATTCAG
 1081 TCACACCTCA AGTACCTAGA ACCATGACTT ACAGGCAGCT GTAGATCTTA GCCCAACTTT
1141 TTCAGAGGA
//
Primer: CMV-Forward RDB12131_A5Ccc.seq
>04387_12131_A5Cc_CMV-Forwaed_F01_16.ab1
    1 CTTCACCTCC GCCCATTGAC GCAATGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA
   61 GCAGCGCGTT TTGCCTGTAC TGGGTCTCTC TGGTTAGACC AGATCTGAGC CTGGGAGCTC
  121 TCTGGCTAAC TAGGGAACCC ACTGCTTAAG CCTCAATAAA GCTTGCCTTG AGTGCTTCAA
  181 GTAGTGTGTG CCCGTCTGTT GTGTGACTCT GGTAACTAGA GATCCCTCAG ACCCTTTTAG
  241 TCAGTGTGGA AAATCTCTAG CAGTGGCGCC CGAACAGGGA CTTGAAAGCG AAAGGGAAAC
  301 CAGAGGAGCT CTCTCGACGC AGGACTCGGC TTGCTGAAGC GCGCACGGCA AGAGGCGAGG
  361 GGCGGCGACT GGTGAGTACG CCAAAAATTT TGACTAGCGG AGGCTAGAAG GAGAGAGATG
  421 GGTGCGAGAG CGTCAGTATT AAGCGGGGGA GAATTAGATC GCGATGGGAA AAAATTCGGT
  481 TAAGGCCAGG GGGAAAGAAA AAATATAAAT TAAAACATAT AGTATGGGCA AGCAGGGAGC
  541 TAGAACGATT CGCAGTTAAT CCTGGCCTGT TAGAAACATC AGAAGGCTGT AGACAAATAC
  601 TGGGACAGCT ACAACCATCC CTTCAGACAG GATCAGAAGA ACTTAGATCA TTATATAATA
  661 CAGTAGCAAC CCTCTATTGT GTGCATCAAA GGATAGAGAT AAAAGACACC AAGGAAGCTT
  721 TAGACAAGAT AGAGGAAGAG CAAAACAAAA GTAAGACCAC CGCACAGCAA GGGCCGCTGA
  781 TCTTCAGACC TGGAGGAGGA GATATGAGGG ACAATTGGAG AAGTGAATTA TTATAAATAA
  841 TAAGTAGTAA AAATTGAAAC CATTAGGAGT AGCACCCACC AAGGCAAAGA GAAGAAGTGG
  901 GTGCAGAGAA GAAAAAAAAG AGCAGTGGGA ATAGGAGCTT TGTTTCTTGG ATTCTTTGGG
  961 AGCAGCAGGA GCACTATGGG GCGCACGGTC ATGACGCTGA CCGTACAGCC AGACAAATTA
 1021 TTTGTCTGCA TAGTGCAGCG CGAACCATCG TGAGGCTATG AGGCCGCAGC ATCTGTGCAC
1081 TCAAGTCTGG GCACTCAAGC AGCTCG
//
Primer: BGH_rev RDB12131_A5Ccd.seq
>04387_12131_A5Cc_BGHrev_G01_19.ab1
    1 GTTCGGACTG CTAGAGATTT TCCACACTGA CTAAAAGGGT CTGAGGGATC TCTAGTTACC
   61 AGAGTCACAC AACAGACGGG CACACACTAC TTGAAGCACT CAAGGCAAGC TTTATTGAGG
  121 CTTAAGCAGT GGGTTCCCTA GTTAGCCAGA GAGCTCCCAG GCTCAGATCT GGTCTAACCA
  181 GAGAGACCCA GTACAGTCCG GATGCAGCTC TCGGGCCATG TGATGAAATG CTAGGCGGCT
  241 GTCAAACCTC CACTCTAATA CTTCTCTCTC CGGGTCATCC ATCCCATGCA GGCTCACAGG
  301 GTGTAACAAG CGGGTGTTCT CTCCTTCATT GGCTTCTTCT ACCTTCTCTT GCTCAACTGG
  361 TACTAGCTTG TAGCACCATC CAAAGGTCAG TGGATATCTG ATCCCTGGCC CTGGTGTGTA
  421 GTTCTGCCAA TCAGGGAAGT AGCCTTGTGT GTGGTAGATC CACAGATCAA GGATATCTTG
  481 TCTTCGTTGG GAGTGAATTA GCCCTTCCAG TCCCCCCTTT TCTTTTAAAA AGTGGCTAAG
  541 ATCTACAGCT GCCTTGTAAG TCATTGGTCT TAAAGGTACC TGAGGTGTGA CTGGAAAACC
  601 CACCTCCTCC TCCTCTTGTG CTTCTAGCCA GGCACAATCA GCATTGGTAG CTGCTGTATT
  661 GCTACTTGTG ATTGCTCCAT GTTTTTCTAG GTCTCGACGG TATCGATGCG GGGAGGCGGC
  721 CCAAAGGGAG ATCCGACTCG TCTGAGGGCG AAGGCGAAGA CGCGGAAGAG GCCGCAGAGC
  781 CGGCAGCAGG CCGCGGGAAG GAAGGTCCGC TGGATTGAGG GCCGAAGGGA CGTAGCAGAA
  841 GGACGTCCCG CGCAGAATCC AGGTGGCAAC ACAGGCGAGC AGCCATGGAA AGGACGTCAG
  901 CTTCCCCGAC AACACCACGG AATTGTCAGT GCCCAACAGC CGAGCCCCTG TCCAGCAGCG
  961 GGCAAAGGCA GGCGGCGATG AGTTCCGCCG TGGCAATAGG GAGGGGGAAG CGAAAGTCCC
 1021 GGAAGGAGCT GACAGGTGGT GGCATGCCCC ACAGTGGGGG GTTGCGTCAG CCAAACACAG
 1081 TGCACCACCA CGACACGTTG CCTGACGACG GAGCACACTC GTCATAAGAA GACAGCACCA
1141 GGAT
//

Please visit plasmidSequencing and PCR primers for primer information.

References and tips

Electronic file

References

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2017.10.23

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