Resource data sheet
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CSII-CMV-RfA

Backbone vector of lentivirus construct by Gateway(R) cloning with CMV promoter.

Catalog number RDB04386
Resource name CSII-CMV-RfA
Clone info. Backbone vector of lentivirus construct by Gateway(R) cloning with CMV promoter.
Use DB3.1 (Invitrogen) or equivalent to amplify this clone. Commonly requested with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Vector backbone CSII-CMV-RfA (Plasmid)
Selectable markers Amp^r, Cm^r, ccdB (E. coli); Zeo^r (plasmid transfection of mammalian cells).
Originator Miyoshi, Hiroyuki |
CLICK here for plasmid list. [link]

Distribution information

Terms and conditions for distribution The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe Dr. Hiroyuki Miyoshi (The Developer, the current address: Keio University) as the Developer. Dr. Miyoshi may request the RECIPIENT to report on progress and/or results obtained through the use of the BIOLOGICAL RESOURCE.
Ordering Please visit Ordering instruction. 
Order form 
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Material Transfer Agreement (MTA) [Word]
Remarks Remember that you will be working with samples containing infectious virus.
Use Survival2 (Invitrogen) or equivalent to amplify this clone.
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB04386 CSII-CMV-RfA DNA solution

Ordering instruction of plasmids [in Japanese] [in English]

References and tips

Electronic file

Electronic file Sequence RDB04386z.seq from Depositor
Electronic file PDF RDB04386.pdf from Depositor

Featured content

References

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB12104_A5D2p1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: HIVgp_R1 RDB12104_A5D2a.seq
>04386_12104_A5D2_HIVgp_R1_A01_01.ab1
    1 CCAGCTCTAT TCCACTGCTC TTTTTTCTCT CTGCACCACT CTTCTCTTTG CCTTGGTGGG
   61 TGCTACTCCT AATGGTTCAA TTTTTACTAC TTTATATTTA TATAATTCAC TTCTCCAATT
  121 GTCCCTCATA TCTCCTCCTC CAGGTCTGAA GATCAGCGGC CGGCCGCTTG CTGTGCGGTG
  181 GTCTTACTTT TGTTTTGCTC TTCCTCTATC TTGTCTAAAG CTTCCTTGGT GTCTTTTATC
  241 TCTATCCTTT GATGCACACA ATAGAGGGTT GCTACTGTAT TATATAATGA TCTAAGTTCT
  301 TCTGATCCTG TCTGAAGGGA TGGTTGTAGC TGTCCCAGTA TTTGTCTACA GCCTTCTGAT
  361 GTTTCTAACA GGCCAGGATT AACTGCGAAT CGTTCTAGCT CCCTGCTTGC CCATACTATA
  421 TGTTTTAATT TATATTTTTT CTTTCCCCCT GGCCTTAACC GAATTTTTTC CCATCGCGAT
  481 CTAATTCTCC CCCGCTTAAT ACTGACGCTC TCGCACCCAT CTCTCTCCTT CTAGCCTCCG
  541 CTAGTCAAAA TTTTTGGCGT ACTCACCAGT CGCCGCCCCT CGCCTCTTGC CGTGCGCGCT
  601 TCAGCAAGCC GAGTCCTGCG TCGAGAGAGC TCCTCTGGTT TCCCTTTCGC TTTCAAGTCC
  661 CTGTTCGGGC GCCACTGCTA GAGATTTTCC ACACTGACTA AAAGGGTCTG AGGGATCTCT
  721 AGTTACCAGA GTCACACAAC AGACGGGCAC ACACTACTTG AAGCACTCAA GGCAAGCTTT
  781 ATTGAGGCTT AAGCAGTGGG TTCCCTAGTT AGCCAGAGAG CTCCCAGGCT CAGATCTGGT
  841 CTAACCAGAG AGACCCAGTA CAGGCAAAAC GCGCTGCTTA TATAGACCTC CCACCGTACA
  901 CGCCTACCGC CCATTTGCGT CAATGGGGCG GAGTTGTTAC GACATTTTGG AAAGTCCCGT
  961 TGATTTTGGT GCCAAAACAA ACTCCCATTG ACGTCAATGG GGGTGGAGAC TTGGAAAATC
 1021 CCCGTGAGTC AAACCGCTAT CCACGCCCAT TGATGTACTG CCAAAACCCG CATCCACCAT
 1081 GGGTAATAGC GATGACTAAT ACCGTAGATG TACCTGGCAG TAGAAGTCCA TAAGTCATGT
1141 ACTTGGGGCA ATTAATTGCC CAGAGGCCG
//
Primer: CSII-CMV5' RDB12104_A5D2b.seq
>04386_12104_A5D2_CSII-CMV5'_B01_04.ab1
    1 ACGCCAAGCG CTACGGGTCT CGAGAATTAT CACAAGTTTG TACAAAAAAG CTGAACGAGA
   61 AACGTAAAAT GATATAAATA TCAATATATT AAATTAGATT TTGCATAAAA AACAGACTAC
  121 ATAATACTGT AAAACACAAC ATATCCAGTC ACTATGGCGG CCGCATTAGG CACCCCAGGC
  181 TTTACACTTT ATGCTTCCGG CTCGTATAAT GTGTGGATTT TGAGTTAGGA TCCGTCGAGA
  241 TTTTCAGGAG CTAAGGAAGC TAAAATGGAG AAAAAAATCA CTGGATATAC CACCGTTGAT
  301 ATATCCCAAT GGCATCGTAA AGAACATTTT GAGGCATTTC AGTCAGTTGC TCAATGTACC
  361 TATAACCAGA CCGTTCAGCT GGATATTACG GCCTTTTTAA AGACCGTAAA GAAAAATAAG
  421 CACAAGTTTT ATCCGGCCTT TATTCACATT CTTGCCCGCC TGATGAATGC TCATCCGGAA
  481 TTCCGTATGG CAATGAAAGA CGGTGAGCTG GTGATATGGG ATAGTGTTCA CCCTTGTTAC
  541 ACCGTTTTCC ATGAGCAAAC TGAAACGTTT TCATCGCTCT GGAGTGAATA CCACGACGAT
  601 TTCCGGCAGT TTCTACACAT ATATTCGCAA GATGTGGCGT GTTACGGTGA AAACCTGGCC
  661 TATTTCCCTA AAGGGTTTAT TGAGAATATG TTTTTCGTCT CAGCCAATCC CTGGGTGAGT
  721 TTCACCAGTT TTGATTTAAA CGTGGCCAAT ATGGACAACT TCTTCGCCCC CGTTTTCACC
  781 ATGGGCAAAT ATTATACGCA AGGCGACAAG GTGCTGATGC CCGCTGGCGA TTCAGGTTCA
  841 TCATGCCGTT TGTGATGGCT TCCATGTCGG CAGAATGCTT AAATGAATTA CAAACAGTAC
  901 TGCGATGAGT GGCAGGGCGG GGCGTAAACG CGTGGATCCG GGCTTACTAA AAGCCAGAAT
  961 AACAGTATGC GTATTTGCGC GCTGGATTTT TTGCGGGTAT AAGAATATAT TACTGGATGT
 1021 GTATACCCCG AAGTTATGTC AAAAAAGGAG GTATGCTATT GAAGCCAGCG TTATTTACAG
 1081 TTGACAGTTG GACAGCGGAC CAGGCTATCA GTTTGGCCTC CAAGGGCATA TATATTGGAA
 1141 TGTCCAATAT TCTTCCGGGT TCTGGGTAAG CACCACCATG TGCCAGAAAT TGGCAAACCC
1201 TCCGGATTCC GTACTCTCGG GTGCGC
//
Primer: ccdB-C_F RDB12104_A5D2c.seq
>04386_12104_A5D2_ccdB-C_F_C01_07.ab1
    1 CATTCGGCGG TGCATATCGG GGATGAAGCT GGCGCATGAT GACCACCGAT ATGGCCAGTG
   61 TGCCGGTCTC CGTTATCGGG GAAGAAGTGG CTGATCTCAG CCACCGCGAA AATGACATCA
  121 AAAACGCCAT TAACCTGATG TTCTGGGGAA TATAAATGTC AGGCTCCCTT ATACACAGCC
  181 AGTCTGCAGG TCGACCATAG TGACTGGATA TGTTGTGTTT TACAGTATTA TGTAGTCTGT
  241 TTTTTATGCA AAATCTAATT TAATATATTG ATATTTATAT CATTTTACGT TTCTCGTTCA
  301 GCTTTCTTGT ACAAAGTGGT GATAATTCTG CAGCGGCCGC GGATCCTCTA GAGTTAACAT
  361 CGAGGGATCA AGCTTATCGA TAATCAACCT CTGGATTACA AAATTTGTGA AAGATTGACT
  421 GGTATTCTTA ACTATGTTGC TCCTTTTACG CTATGTGGAT ACGCTGCTTT AATGCCTTTG
  481 TATCATGCTA TTGCTTCCCG TATGGCTTTC ATTTTCTCCT CCTTGTATAA ATCCTGGTTG
  541 CTGTCTCTTT ATGAGGAGTT GTGGCCCGTT GTCAGGCAAC GTGGCGTGGT GTGCACTGTG
  601 TTTGCTGACG CAACCCCCAC TGGTTGGGGC ATTGCCACCA CCTGTCAGCT CCTTTCCGGG
  661 ACTTTCGCTT TCCCCCTCCC TATTGCCACG GCGGAACTCA TCGCCGCCTG CCTTGCCCGC
  721 TGCTGGACAG GGGCTCGGCT GTTGGGCACT GACAATTCCG TGGTGTTGTC GGGGAAGCTG
  781 ACGTCCTTTC CATGGCTGCT CGCCTGTGTT GCCACCTGGA TTCTGCGCGG GACGTCCTTC
  841 TGCTACGTCC CTTCGGCCCT CAATCCAGCG GACCTTCCTT CCCGCGGCCT GCTGCCGGCT
  901 CTGCGGCCTC TTCCGCGTCT TCGCCTTCGC CCTCAGACGA GTCGGATCTC CCTTTGGGCC
  961 GCCTCCCCGC ATCGATACCG TCGAGACCTA GAAAACATGG AGCAAATCAC AAGTAGCAAT
 1021 ACAGCAGCTA CCAAATGCTG ATTGTGCCTG GCTAGAAGCA CAGAGGAGGG AGGAGGTGGG
 1081 TTTTCAGTCA CCACCTCAGT ACCTTAGGAT AATGGACCTA CAGCAGGCTG TAGAATTCTT
1141 AGCCACTTTT TTAAAAGGA
//
Primer: BGH_rev RDB12104_A5D2d.seq
>04386_12104_A5D2_BGHrerv_D01_10.ab1
    1 CTTTCGGGGA CCTGCTAGAG ATTTTCCACA CTGACTAAAA GGGTCTGAGG GATCTCTAGT
   61 TACCAGAGTC ACACAACAGA CGGGCACACA CTACTTGAAG CACTCAAGGC AAGCTTTATT
  121 GAGGCTTAAG CAGTGGGTTC CCTAGTTAGC CAGAGAGCTC CCAGGCTCAG ATCTGGTCTA
  181 ACCAGAGAGA CCCAGTACAG TCCGGATGCA GCTCTCGGGC CATGTGATGA AATGCTAGGC
  241 GGCTGTCAAA CCTCCACTCT AATACTTCTC TCTCCGGGTC ATCCATCCCA TGCAGGCTCA
  301 CAGGGTGTAA CAAGCGGGTG TTCTCTCCTT CATTGGCTTC TTCTACCTTC TCTTGCTCAA
  361 CTGGTACTAG CTTGTAGCAC CATCCAAAGG TCAGTGGATA TCTGATCCCT GGCCCTGGTG
  421 TGTAGTTCTG CCAATCAGGG AAGTAGCCTT GTGTGTGGTA GATCCACAGA TCAAGGATAT
  481 CTTGTCTTCG TTGGGAGTGA ATTAGCCCTT CCAGTCCCCC CTTTTCTTTT AAAAAGTGGC
  541 TAAGATCTAC AGCTGCCTTG TAAGTCATTG GTCTTAAAGG TACCTGAGGT GTGACTGGAA
  601 AACCCACCTC CTCCTCCTCT TGTGCTTCTA GCCAGGCACA ATCAGCATTG GTAGCTGCTG
  661 TATTGCTACT TGTGATTGCT CCATGTTTTT CTAGGTCTCG ACGGTATCGA TGCGGGGAGG
  721 CGGCCCAAAG GGAGATCCGA CTCGTCTGAG GGCGAAGGCG AAGACGCGGA AGAGGCCGCA
  781 GAGCCGGCAG CAGGCCGCGG GAAGGAAGGT CCGCTGGATT GAGGGCCGAA GGGACGTAGC
  841 AGAAGGACGT CCCGCGCAGA ATCCAGGTGG CAACACAGGC GAGCAGCCAT GGAAAGGACG
  901 TCAGCTTCCC CGACAACACC ACGGAATTGT CAGTGCCCAA CAGCCGAGCC CCTGTCCAGC
  961 AGCGGGCAAG GCAGGCGGCG ATGAGTTCCG CCGTGGCAAT AGGGAGGGGG AAAGCGAAGT
 1021 CCCGGGAAGG AGCTGACAGT GGTGGCAATG CCCCAACCCA GTGGGGGGTT GCGTCAGCAA
 1081 ACACAGTGCA CACCACCGCC ACGTTTGCCT TGACACCGGA CCACACTCTT CATAAGAGAC
1141 AGCAACCAAG GTATTTATAT ACAAAGGAAA GGA
//

Please visit Sequencing and PCR primers for primer information.


References

References

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user report Noguchi, K., Molecular analysis of keratocystic odontogenic tumor cell lines derived from sporadic and basal cell nevus syndrome patients. Int. J. Oncol. 51 (6): 1731-1738 (2017). PMID 29039452.
user report Fuchigami, T., Fibroblasts promote the collective invasion of ameloblastoma tumor cells in a 3D coculture model. FEBS Open Bio. 7 (12): 2000-2007 (2017). PMID 29226086.
user report Yachida, S., Genomic Sequencing Identifies ELF3 as a Driver of Ampullary Carcinoma. Cancer Cell pii: S1535-6108 (15) 00476-6 (2016). PMID 26806338.
user report Higuchi, M., USP10 Is an Essential Deubiquitinase for Hematopoiesis and Inhibits Apoptosis of Long-Term Hematopoietic Stem Cells. Stem Cell Reports 7 (6): 1116-1129 (2016). PMID 27974222.
user report Donai, K., Bovine and porcine fibroblasts can be immortalized with intact karyotype by the expression of mutant cyclin dependent kinase 4, cyclin D, and telomerase. J. Biotechnol., 176: 50-57 (2014). PMID 24589663.
user report Inagawa, Y., A human cancer xenograft model utilizing normal pancreatic duct epithelial cells conditionally transformed with defined oncogenes. Carcinogenesis 35 (8): 1840-1846 (2014). PMID 24858378.
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user report Moriyama, H., Tightly regulated and homogeneous transgene expression in human adipose-derived mesenchymal stem cells by lentivirus with tet-off system. PLoS One, 8 (6): e66274 (2013). PMID 23776652.
user report Sasaki, R., Oncogenic transformation of human ovarian surface epithelial cells with defined cellular oncogenes. Carcinogenesis, 30 (3): 423-431 (2012). PMID 19126650.
user report Egawa, N., The E1 protein of human papillomavirus type 16 is dispensable for maintenance replication of the viral genome. J. Virol., 86 (6): 3276-3283 (2012). PMID 22238312.
user report Harao M., HLA-A2-restricted CTL epitopes of a novel lung cancer-associated cancer testis antigen, cell division cycle associated 1, can induce tumor-reactive CTL. Int. J. Cancer., 123, 2616-2625 (2008). PMID 18770861.
user report Imai K., Identification of a novel tumor-associated antigen, cadherin 3/P-cadherin, as a possible target for immunotherapy of pancreatic, gastric, and colorectal cancers. Clin. Cancer. Res., 14, 6487-6495 (2008). PMID 18927288.
user report Irie A., Protein kinase D2 contributes to either IL-2 promoter regulation or induction of cell death upon TCR stimulation depending on its activity in Jurkat cells. Int. Immunol., 18, 1737-1747 (2006). PMID 17077180.

2018.10.22

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