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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

CSII-CMV-RfA

Backbone vector plasmid of lentivirus construct by Gateway(R) cloning with CMV promoter.

Catalog number RDB04386
Resource name CSII-CMV-RfA
Clone info. Backbone vector plasmid of lentivirus construct by Gateway(R) cloning with CMV promoter.
Comment Commonly requested with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Vector backbone CSII-CMV-RfA (Plasmid)
Selectable markers Please note that Zeo resistance marker is not included in the lentivirus produced from this vector. E. coli: Amp^r, Cm^r, ccdB.
Growth remarks Use DB3.1, ccdB Survival or equivalent to amplify this clone. 30 degC is fine.
Depositor|Developer Miyoshi, Hiroyuki |
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External Database

            Reference sequence
              
            Lentiviral plasmid list. [link]

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer.
            Ordering Please visit Ordering instruction.[link] 
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            Material Transfer Agreement (MTA, for use for not-for-profit academic purpose)[Word]
            Remarks Remember that you will be working with samples containing infectious virus.
            Use Survival2 (Invitrogen) or equivalent to amplify this clone.
            提供案内 (日本国内) [open/close]

            提供条件 本件研究材料は、非営利機関の非営利学術研究に限って提供する。本件研究材料を利用した研究結果等を発表する際は、本件研究材料が故三好浩之博士により開発されたことを明示する。
            提供依頼 手続きの詳細は、「レンチウイルスベクターの提供申し込み」をご覧ください。
            提供依頼書 [Word]
            提供同意書 (非営利機関による非営利学術研究用)[Word]
            遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。
            備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。
            このクローンの増殖にはSurvival2 (Invitrogen)あるいは同等の宿主を使用してください。

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB04386 CSII-CMV-RfA DNA solution

            Ordering instruction of plasmids [in Japanese] [in English]
            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Electronic file

            Sequence RDB04386z.seq
            Remarks, protocol and/or map (pdf) RDB04386.pdf

            Original reference

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Lentivirus Vector (English text)
            Featured content Lentivirus Vector (Japanese text)

            Sequence information

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB12104_A5D2p1-3.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: CSII-CMV5'
            Region: attR1,CmR
            Sequence file: RDB12104_A5D2f.seq check
            >04386_12104_A5D2_HIVgp_R1_A01_01.ab1
                1 CCAGCTCTAT TCCACTGCTC TTTTTTCTCT CTGCACCACT CTTCTCTTTG CCTTGGTGGG
               61 TGCTACTCCT AATGGTTCAA TTTTTACTAC TTTATATTTA TATAATTCAC TTCTCCAATT
              121 GTCCCTCATA TCTCCTCCTC CAGGTCTGAA GATCAGCGGC CGGCCGCTTG CTGTGCGGTG
              181 GTCTTACTTT TGTTTTGCTC TTCCTCTATC TTGTCTAAAG CTTCCTTGGT GTCTTTTATC
              241 TCTATCCTTT GATGCACACA ATAGAGGGTT GCTACTGTAT TATATAATGA TCTAAGTTCT
              301 TCTGATCCTG TCTGAAGGGA TGGTTGTAGC TGTCCCAGTA TTTGTCTACA GCCTTCTGAT
              361 GTTTCTAACA GGCCAGGATT AACTGCGAAT CGTTCTAGCT CCCTGCTTGC CCATACTATA
              421 TGTTTTAATT TATATTTTTT CTTTCCCCCT GGCCTTAACC GAATTTTTTC CCATCGCGAT
              481 CTAATTCTCC CCCGCTTAAT ACTGACGCTC TCGCACCCAT CTCTCTCCTT CTAGCCTCCG
              541 CTAGTCAAAA TTTTTGGCGT ACTCACCAGT CGCCGCCCCT CGCCTCTTGC CGTGCGCGCT
              601 TCAGCAAGCC GAGTCCTGCG TCGAGAGAGC TCCTCTGGTT TCCCTTTCGC TTTCAAGTCC
              661 CTGTTCGGGC GCCACTGCTA GAGATTTTCC ACACTGACTA AAAGGGTCTG AGGGATCTCT
              721 AGTTACCAGA GTCACACAAC AGACGGGCAC ACACTACTTG AAGCACTCAA GGCAAGCTTT
              781 ATTGAGGCTT AAGCAGTGGG TTCCCTAGTT AGCCAGAGAG CTCCCAGGCT CAGATCTGGT
              841 CTAACCAGAG AGACCCAGTA CAGGCAAAAC GCGCTGCTTA TATAGACCTC CCACCGTACA
              901 CGCCTACCGC CCATTTGCGT CAATGGGGCG GAGTTGTTAC GACATTTTGG AAAGTCCCGT
              961 TGATTTTGGT GCCAAAACAA ACTCCCATTG ACGTCAATGG GGGTGGAGAC TTGGAAAATC
             1021 CCCGTGAGTC AAACCGCTAT CCACGCCCAT TGATGTACTG CCAAAACCCG CATCCACCAT
             1081 GGGTAATAGC GATGACTAAT ACCGTAGATG TACCTGGCAG TAGAAGTCCA TAAGTCATGT
            1141 ACTTGGGGCA ATTAATTGCC CAGAGGCCG
            //
            Primer: ccdB-C_F
            Region: ccdB,attR2,PRE
            Sequence file: RDB12104_A5D2g.seq check
            >04386_12104_A5D2_CSII-CMV5'_B01_04.ab1
                1 ACGCCAAGCG CTACGGGTCT CGAGAATTAT CACAAGTTTG TACAAAAAAG CTGAACGAGA
               61 AACGTAAAAT GATATAAATA TCAATATATT AAATTAGATT TTGCATAAAA AACAGACTAC
              121 ATAATACTGT AAAACACAAC ATATCCAGTC ACTATGGCGG CCGCATTAGG CACCCCAGGC
              181 TTTACACTTT ATGCTTCCGG CTCGTATAAT GTGTGGATTT TGAGTTAGGA TCCGTCGAGA
              241 TTTTCAGGAG CTAAGGAAGC TAAAATGGAG AAAAAAATCA CTGGATATAC CACCGTTGAT
              301 ATATCCCAAT GGCATCGTAA AGAACATTTT GAGGCATTTC AGTCAGTTGC TCAATGTACC
              361 TATAACCAGA CCGTTCAGCT GGATATTACG GCCTTTTTAA AGACCGTAAA GAAAAATAAG
              421 CACAAGTTTT ATCCGGCCTT TATTCACATT CTTGCCCGCC TGATGAATGC TCATCCGGAA
              481 TTCCGTATGG CAATGAAAGA CGGTGAGCTG GTGATATGGG ATAGTGTTCA CCCTTGTTAC
              541 ACCGTTTTCC ATGAGCAAAC TGAAACGTTT TCATCGCTCT GGAGTGAATA CCACGACGAT
              601 TTCCGGCAGT TTCTACACAT ATATTCGCAA GATGTGGCGT GTTACGGTGA AAACCTGGCC
              661 TATTTCCCTA AAGGGTTTAT TGAGAATATG TTTTTCGTCT CAGCCAATCC CTGGGTGAGT
              721 TTCACCAGTT TTGATTTAAA CGTGGCCAAT ATGGACAACT TCTTCGCCCC CGTTTTCACC
              781 ATGGGCAAAT ATTATACGCA AGGCGACAAG GTGCTGATGC CCGCTGGCGA TTCAGGTTCA
              841 TCATGCCGTT TGTGATGGCT TCCATGTCGG CAGAATGCTT AAATGAATTA CAAACAGTAC
              901 TGCGATGAGT GGCAGGGCGG GGCGTAAACG CGTGGATCCG GGCTTACTAA AAGCCAGAAT
              961 AACAGTATGC GTATTTGCGC GCTGGATTTT TTGCGGGTAT AAGAATATAT TACTGGATGT
             1021 GTATACCCCG AAGTTATGTC AAAAAAGGAG GTATGCTATT GAAGCCAGCG TTATTTACAG
             1081 TTGACAGTTG GACAGCGGAC CAGGCTATCA GTTTGGCCTC CAAGGGCATA TATATTGGAA
             1141 TGTCCAATAT TCTTCCGGGT TCTGGGTAAG CACCACCATG TGCCAGAAAT TGGCAAACCC
            1201 TCCGGATTCC GTACTCTCGG GTGCGC
            //
            Primer: HIVgp_R1
            Region: HIV-1 psi,5'LTR,CMV pro
            Sequence file: RDB12104_A5D2h.seq check
            >04386_12104_A5D2_ccdB-C_F_C01_07.ab1
                1 CATTCGGCGG TGCATATCGG GGATGAAGCT GGCGCATGAT GACCACCGAT ATGGCCAGTG
               61 TGCCGGTCTC CGTTATCGGG GAAGAAGTGG CTGATCTCAG CCACCGCGAA AATGACATCA
              121 AAAACGCCAT TAACCTGATG TTCTGGGGAA TATAAATGTC AGGCTCCCTT ATACACAGCC
              181 AGTCTGCAGG TCGACCATAG TGACTGGATA TGTTGTGTTT TACAGTATTA TGTAGTCTGT
              241 TTTTTATGCA AAATCTAATT TAATATATTG ATATTTATAT CATTTTACGT TTCTCGTTCA
              301 GCTTTCTTGT ACAAAGTGGT GATAATTCTG CAGCGGCCGC GGATCCTCTA GAGTTAACAT
              361 CGAGGGATCA AGCTTATCGA TAATCAACCT CTGGATTACA AAATTTGTGA AAGATTGACT
              421 GGTATTCTTA ACTATGTTGC TCCTTTTACG CTATGTGGAT ACGCTGCTTT AATGCCTTTG
              481 TATCATGCTA TTGCTTCCCG TATGGCTTTC ATTTTCTCCT CCTTGTATAA ATCCTGGTTG
              541 CTGTCTCTTT ATGAGGAGTT GTGGCCCGTT GTCAGGCAAC GTGGCGTGGT GTGCACTGTG
              601 TTTGCTGACG CAACCCCCAC TGGTTGGGGC ATTGCCACCA CCTGTCAGCT CCTTTCCGGG
              661 ACTTTCGCTT TCCCCCTCCC TATTGCCACG GCGGAACTCA TCGCCGCCTG CCTTGCCCGC
              721 TGCTGGACAG GGGCTCGGCT GTTGGGCACT GACAATTCCG TGGTGTTGTC GGGGAAGCTG
              781 ACGTCCTTTC CATGGCTGCT CGCCTGTGTT GCCACCTGGA TTCTGCGCGG GACGTCCTTC
              841 TGCTACGTCC CTTCGGCCCT CAATCCAGCG GACCTTCCTT CCCGCGGCCT GCTGCCGGCT
              901 CTGCGGCCTC TTCCGCGTCT TCGCCTTCGC CCTCAGACGA GTCGGATCTC CCTTTGGGCC
              961 GCCTCCCCGC ATCGATACCG TCGAGACCTA GAAAACATGG AGCAAATCAC AAGTAGCAAT
             1021 ACAGCAGCTA CCAAATGCTG ATTGTGCCTG GCTAGAAGCA CAGAGGAGGG AGGAGGTGGG
             1081 TTTTCAGTCA CCACCTCAGT ACCTTAGGAT AATGGACCTA CAGCAGGCTG TAGAATTCTT
            1141 AGCCACTTTT TTAAAAGGA
            //
            Primer: BGH_rev
            Region: 3'LTR,PRE
            Sequence file: RDB12104_A5D2i.seq check
            >04386_12104_A5D2_BGHrerv_D01_10.ab1
                1 CTTTCGGGGA CCTGCTAGAG ATTTTCCACA CTGACTAAAA GGGTCTGAGG GATCTCTAGT
               61 TACCAGAGTC ACACAACAGA CGGGCACACA CTACTTGAAG CACTCAAGGC AAGCTTTATT
              121 GAGGCTTAAG CAGTGGGTTC CCTAGTTAGC CAGAGAGCTC CCAGGCTCAG ATCTGGTCTA
              181 ACCAGAGAGA CCCAGTACAG TCCGGATGCA GCTCTCGGGC CATGTGATGA AATGCTAGGC
              241 GGCTGTCAAA CCTCCACTCT AATACTTCTC TCTCCGGGTC ATCCATCCCA TGCAGGCTCA
              301 CAGGGTGTAA CAAGCGGGTG TTCTCTCCTT CATTGGCTTC TTCTACCTTC TCTTGCTCAA
              361 CTGGTACTAG CTTGTAGCAC CATCCAAAGG TCAGTGGATA TCTGATCCCT GGCCCTGGTG
              421 TGTAGTTCTG CCAATCAGGG AAGTAGCCTT GTGTGTGGTA GATCCACAGA TCAAGGATAT
              481 CTTGTCTTCG TTGGGAGTGA ATTAGCCCTT CCAGTCCCCC CTTTTCTTTT AAAAAGTGGC
              541 TAAGATCTAC AGCTGCCTTG TAAGTCATTG GTCTTAAAGG TACCTGAGGT GTGACTGGAA
              601 AACCCACCTC CTCCTCCTCT TGTGCTTCTA GCCAGGCACA ATCAGCATTG GTAGCTGCTG
              661 TATTGCTACT TGTGATTGCT CCATGTTTTT CTAGGTCTCG ACGGTATCGA TGCGGGGAGG
              721 CGGCCCAAAG GGAGATCCGA CTCGTCTGAG GGCGAAGGCG AAGACGCGGA AGAGGCCGCA
              781 GAGCCGGCAG CAGGCCGCGG GAAGGAAGGT CCGCTGGATT GAGGGCCGAA GGGACGTAGC
              841 AGAAGGACGT CCCGCGCAGA ATCCAGGTGG CAACACAGGC GAGCAGCCAT GGAAAGGACG
              901 TCAGCTTCCC CGACAACACC ACGGAATTGT CAGTGCCCAA CAGCCGAGCC CCTGTCCAGC
              961 AGCGGGCAAG GCAGGCGGCG ATGAGTTCCG CCGTGGCAAT AGGGAGGGGG AAAGCGAAGT
             1021 CCCGGGAAGG AGCTGACAGT GGTGGCAATG CCCCAACCCA GTGGGGGGTT GCGTCAGCAA
             1081 ACACAGTGCA CACCACCGCC ACGTTTGCCT TGACACCGGA CCACACTCTT CATAAGAGAC
            1141 AGCAACCAAG GTATTTATAT ACAAAGGAAA GGA
            //
            Primer: SV40pro_F
            Region: SV40pro_ori,Zeo,SV40pA
            Sequence file: RDB12104_A5D2j.seq check
            >04386_12104_A5D2_SV40pro_F_E01_13.ab1
                1 CCTTGTTCCC TTGGGCATTC AGAAGTAGTG AGGAGGCTTT TTTGGAGGCC TAGCGCTTTT
               61 GCAAAAAGCT CCCGGGAGCT TGTATATCCA TTTTCGGATC TGATCAGCAC GTGTTGACAA
              121 TTAATCATCG GCATAGTATA TCGGCATAGT ATAATACGAC AAGGTGAGGA ACTAAACCAT
              181 GGCCAAGTTG ACCAGTGCCG TTCCGGTGCT CACCGCGCGC GACGTCGCCG GAGCGGTCGA
              241 GTTCTGGACC GACCGGCTCG GGTTCTCCCG GGACTTCGTG GAGGACGACT TCGCCGGTGT
              301 GGTCCGGGAC GACGTGACCC TGTTCATCAG CGCGGTCCAG GACCAGGTGG TGCCGGACAA
              361 CACCCTGGCC TGGGTGTGGG TGCGCGGCCT GGACGAGCTG TACGCCGAGT GGTCGGAGGT
              421 CGTGTCCACG AACTTCCGGG ACGCCTCCGG GCCGGCCATG ACCGAGATCG GCGAGCAGCC
              481 GTGGGGGCGG GAGTTCGCCC TGCGCGACCC GGCCGGCAAC TGCGTGCACT TCGTGGCCGA
              541 GGAGCAGGAC TGACACGTGC TACGAGATTT CGATTCCACC GCCGCCTTCT ATGAAAGGTT
              601 GGGCTTCGGA ATCGTTTTCC GGGACGCCGG CTGGATGATC CTCCAGCGCG GGGATCTCAT
              661 GCTGGAGTTC TTCGCCCACC CCAACTTGTT TATTGCAGCT TATAATGGTT ACAAATAAAG
              721 CAATAGCATC ACAAATTTCA CAAATAAAGC ATTTTTTTCA CTGCATTCTA GTTGTGGTTT
              781 GTCCAAACTC ATCAATGTAT CTTATCATGT CTGTATACCG TCGACCTCTA GCTAGAGCTT
              841 GGCGTAATCA TGGTCATAGC TGTTTCCTGT GTGAAATTGT TATCCGCTCA CAATTCCACA
              901 CAACATACGA GCCGGAAGCA TAAAGTGTAA AGCCTGGGGT GCCTAATGAG TGAGCTAACT
              961 CACATTAATT GCGTTGCGCT CACTGCCCGC TTTCCAGTCG GGAAACCTGT CGTGCCAGCT
             1021 GCATTAATGA ATCGGGCCAA CGCGCGGGGA GAGGCGGGTT TGCGTATTGG GCGCTCTTCC
             1081 CGCTCTTCGC TCCACTGACT CGCTGCGCTT CGCTCGCTTC GGCTGCGGCG AGCCGTATCA
            1141 GCTCACTTCA AAGGGCGCGG TTAATTACCG G
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            user_report Yasukawa, Y., SAA1 is upregulated in gastric cancer-associated fibroblasts possibly by its enhancer activation. Carcinogenesis 42 (2): 180-189 (2021). PMID 33284950.
            user_report Miyakuni, K., Genome-wide analysis of DNA methylation identifies the apoptosis-related gene UQCRH as a tumor suppressor in renal cancer. Mol Oncol. 2021 Jun 16. doi: 10.1002/1878-0261.13040. Epub ahead of print. PMID 34133843.
            user_report Zhou, L., The beta-galactoside alpha2,6-sialyltranferase 1 (ST6GAL1) inhibits the colorectal cancer metastasis by stabilizing intercellular adhesion molecule-1 via sialylation. Cancer Manag. Res. 11: 6185-6199 (2019). PMID 31308754.
            user_report Inoue, S., Uterine adenomyosis is an oligoclonal disorder associated with KRAS mutations. Nat. Commun. 10 (1): 5785 (2019). PMID 31857578.
            user_report Shinohara, M., Tumor necrosis factor-alpha inhibits differentiation of myogenic cells in human urethral rhabdosphincter. Int. J. Urol. 24 (6): 461-467 (2017). PMID 28332237.
            user_report Kubota, S.I., Whole-Body Profiling of Cancer Metastasis with Single-Cell Resolution. Cell Rep. 20 (1): 236-250 (2017). PMID 28683317.
            user_report Noguchi, K., Molecular analysis of keratocystic odontogenic tumor cell lines derived from sporadic and basal cell nevus syndrome patients. Int. J. Oncol. 51 (6): 1731-1738 (2017). PMID 29039452.
            user_report Fuchigami, T., Fibroblasts promote the collective invasion of ameloblastoma tumor cells in a 3D coculture model. FEBS Open Bio. 7 (12): 2000-2007 (2017). PMID 29226086.
            user_report Yachida, S., Genomic Sequencing Identifies ELF3 as a Driver of Ampullary Carcinoma. Cancer Cell pii: S1535-6108 (15) 00476-6 (2016). PMID 26806338.
            user_report Higuchi, M., USP10 Is an Essential Deubiquitinase for Hematopoiesis and Inhibits Apoptosis of Long-Term Hematopoietic Stem Cells. Stem Cell Reports 7 (6): 1116-1129 (2016). PMID 27974222.
            user_report Donai, K., Bovine and porcine fibroblasts can be immortalized with intact karyotype by the expression of mutant cyclin dependent kinase 4, cyclin D, and telomerase. J. Biotechnol., 176: 50-57 (2014). PMID 24589663.
            user_report Inagawa, Y., A human cancer xenograft model utilizing normal pancreatic duct epithelial cells conditionally transformed with defined oncogenes. Carcinogenesis 35 (8): 1840-1846 (2014). PMID 24858378.
            user_report Muraoka, N., MiR-133 promotes cardiac reprogramming by directly repressing Snai1 and silencing fibroblast signatures. EMBO J., 33 (14): 1565-1581 (2014). PMID 24920580.
            user_report Kibe, T., A novel ameloblastoma cell line (AM-3) secretes MMP-9 in response to Wnt-3a and induces osteoclastogenesis. Oral Surg. Oral Med. Oral Pathol. Oral Radiol., 115 (6): 780-788 (2013). PMID 23706918.
            user_report Moriyama, H., Tightly regulated and homogeneous transgene expression in human adipose-derived mesenchymal stem cells by lentivirus with tet-off system. PLoS One, 8 (6): e66274 (2013). PMID 23776652.
            user_report Sasaki, R., Oncogenic transformation of human ovarian surface epithelial cells with defined cellular oncogenes. Carcinogenesis, 30 (3): 423-431 (2012). PMID 19126650.
            user_report Egawa, N., The E1 protein of human papillomavirus type 16 is dispensable for maintenance replication of the viral genome. J. Virol., 86 (6): 3276-3283 (2012). PMID 22238312.
            user_report Kibe, T., Immortalization and characterization of normal oral epithelial cells without using HPV and SV40 genes. Oral Science Internatl., 8 (1): 20-28, 2011.
            user_report Harao M., HLA-A2-restricted CTL epitopes of a novel lung cancer-associated cancer testis antigen, cell division cycle associated 1, can induce tumor-reactive CTL. Int. J. Cancer., 123, 2616-2625 (2008). PMID 18770861.
            user_report Imai K., Identification of a novel tumor-associated antigen, cadherin 3/P-cadherin, as a possible target for immunotherapy of pancreatic, gastric, and colorectal cancers. Clin. Cancer. Res., 14, 6487-6495 (2008). PMID 18927288.
            user_report Irie A., Protein kinase D2 contributes to either IL-2 promoter regulation or induction of cell death upon TCR stimulation depending on its activity in Jurkat cells. Int. Immunol., 18, 1737-1747 (2006). PMID 17077180.

            2022.05.18

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