RDB04386 - CSII-CMV-RfA

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CSII-CMV-RfA

Backbone vector plasmid of lentivirus construct by Gateway(R) cloning with CMV promoter.

Catalog number	RDB04386
Resource name	CSII-CMV-RfA
Clone info.	Backbone vector plasmid of lentivirus construct by Gateway(R) cloning with CMV promoter.
Comment	Commonly requested with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Vector backbone	CSII-CMV-RfA (Plasmid)
Selectable markers	Please note that Zeo resistance marker is not included in the lentivirus produced from this vector. E. coli: Amp^r, Cm^r, ccdB.
Growth remarks	Use DB3.1, ccdB Survival or equivalent to amplify this clone. 30 degC is fine.
Depositor\|Developer	Miyoshi, Hiroyuki \|

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External Database

Reference sequence

Sequence	RDB04386z.seq
Remarks, protocol and/or map (pdf)	RDB04386.pdf

Lentiviral plasmid list. [link]

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer.

Ordering Please visit Ordering instruction.[link]
Order form [Credit Card Payment] [Bank Transfer Payment]
Material Transfer Agreement (MTA, for use for not-for-profit academic purpose)[Word]

Remarks Remember that you will be working with samples containing infectious virus.
Use Survival2 (Invitrogen) or equivalent to amplify this clone.

提供案内 (日本国内) [open/close]

Terms and conditions for distribution	The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer.
Ordering	Please visit Ordering instruction.[link] Order form [Credit Card Payment] [Bank Transfer Payment] Material Transfer Agreement (MTA, for use for not-for-profit academic purpose)[Word]
Remarks	Remember that you will be working with samples containing infectious virus. Use Survival2 (Invitrogen) or equivalent to amplify this clone.

提供条件	本件研究材料は、非営利機関の非営利学術研究に限って提供する。本件研究材料を利用した研究結果等を発表する際は、本件研究材料が故三好浩之博士により開発されたことを明示する。
提供依頼	手続きの詳細は、「レンチウイルスベクターの提供申し込み」をご覧ください。提供依頼書 [Word] 提供同意書 (非営利機関による非営利学術研究用)[Word] 遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。
備考	このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。このクローンの増殖にはSurvival2 (Invitrogen)あるいは同等の宿主を使用してください。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB04386	CSII-CMV-RfA	DNA solution

Ordering instruction of plasmids [in Japanese] [in English]
Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The CSII-CMV-RfA was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB04386).

Reference section:

Further references such as user reports and related articles (go to bottom)

References and tips

Featured content	Lentivirus Vector (English text)
Featured content	Lentivirus Vector (Japanese text)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB12104_A5D2p1-3.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CSII-CMV5' (Pr0021) Region: attR1,CmR Sequence file: RDB12104_A5D2f.seq
>04386_12104_A5D2_HIVgp_R1_A01_01.ab1 1 CCAGCTCTAT TCCACTGCTC TTTTTTCTCT CTGCACCACT CTTCTCTTTG CCTTGGTGGG 61 TGCTACTCCT AATGGTTCAA TTTTTACTAC TTTATATTTA TATAATTCAC TTCTCCAATT 121 GTCCCTCATA TCTCCTCCTC CAGGTCTGAA GATCAGCGGC CGGCCGCTTG CTGTGCGGTG 181 GTCTTACTTT TGTTTTGCTC TTCCTCTATC TTGTCTAAAG CTTCCTTGGT GTCTTTTATC 241 TCTATCCTTT GATGCACACA ATAGAGGGTT GCTACTGTAT TATATAATGA TCTAAGTTCT 301 TCTGATCCTG TCTGAAGGGA TGGTTGTAGC TGTCCCAGTA TTTGTCTACA GCCTTCTGAT 361 GTTTCTAACA GGCCAGGATT AACTGCGAAT CGTTCTAGCT CCCTGCTTGC CCATACTATA 421 TGTTTTAATT TATATTTTTT CTTTCCCCCT GGCCTTAACC GAATTTTTTC CCATCGCGAT 481 CTAATTCTCC CCCGCTTAAT ACTGACGCTC TCGCACCCAT CTCTCTCCTT CTAGCCTCCG 541 CTAGTCAAAA TTTTTGGCGT ACTCACCAGT CGCCGCCCCT CGCCTCTTGC CGTGCGCGCT 601 TCAGCAAGCC GAGTCCTGCG TCGAGAGAGC TCCTCTGGTT TCCCTTTCGC TTTCAAGTCC 661 CTGTTCGGGC GCCACTGCTA GAGATTTTCC ACACTGACTA AAAGGGTCTG AGGGATCTCT 721 AGTTACCAGA GTCACACAAC AGACGGGCAC ACACTACTTG AAGCACTCAA GGCAAGCTTT 781 ATTGAGGCTT AAGCAGTGGG TTCCCTAGTT AGCCAGAGAG CTCCCAGGCT CAGATCTGGT 841 CTAACCAGAG AGACCCAGTA CAGGCAAAAC GCGCTGCTTA TATAGACCTC CCACCGTACA 901 CGCCTACCGC CCATTTGCGT CAATGGGGCG GAGTTGTTAC GACATTTTGG AAAGTCCCGT 961 TGATTTTGGT GCCAAAACAA ACTCCCATTG ACGTCAATGG GGGTGGAGAC TTGGAAAATC 1021 CCCGTGAGTC AAACCGCTAT CCACGCCCAT TGATGTACTG CCAAAACCCG CATCCACCAT 1081 GGGTAATAGC GATGACTAAT ACCGTAGATG TACCTGGCAG TAGAAGTCCA TAAGTCATGT 1141 ACTTGGGGCA ATTAATTGCC CAGAGGCCG //
Primer: ccdB-C_F (Pr0136) Region: ccdB,attR2,PRE Sequence file: RDB12104_A5D2g.seq
>04386_12104_A5D2_CSII-CMV5'_B01_04.ab1 1 ACGCCAAGCG CTACGGGTCT CGAGAATTAT CACAAGTTTG TACAAAAAAG CTGAACGAGA 61 AACGTAAAAT GATATAAATA TCAATATATT AAATTAGATT TTGCATAAAA AACAGACTAC 121 ATAATACTGT AAAACACAAC ATATCCAGTC ACTATGGCGG CCGCATTAGG CACCCCAGGC 181 TTTACACTTT ATGCTTCCGG CTCGTATAAT GTGTGGATTT TGAGTTAGGA TCCGTCGAGA 241 TTTTCAGGAG CTAAGGAAGC TAAAATGGAG AAAAAAATCA CTGGATATAC CACCGTTGAT 301 ATATCCCAAT GGCATCGTAA AGAACATTTT GAGGCATTTC AGTCAGTTGC TCAATGTACC 361 TATAACCAGA CCGTTCAGCT GGATATTACG GCCTTTTTAA AGACCGTAAA GAAAAATAAG 421 CACAAGTTTT ATCCGGCCTT TATTCACATT CTTGCCCGCC TGATGAATGC TCATCCGGAA 481 TTCCGTATGG CAATGAAAGA CGGTGAGCTG GTGATATGGG ATAGTGTTCA CCCTTGTTAC 541 ACCGTTTTCC ATGAGCAAAC TGAAACGTTT TCATCGCTCT GGAGTGAATA CCACGACGAT 601 TTCCGGCAGT TTCTACACAT ATATTCGCAA GATGTGGCGT GTTACGGTGA AAACCTGGCC 661 TATTTCCCTA AAGGGTTTAT TGAGAATATG TTTTTCGTCT CAGCCAATCC CTGGGTGAGT 721 TTCACCAGTT TTGATTTAAA CGTGGCCAAT ATGGACAACT TCTTCGCCCC CGTTTTCACC 781 ATGGGCAAAT ATTATACGCA AGGCGACAAG GTGCTGATGC CCGCTGGCGA TTCAGGTTCA 841 TCATGCCGTT TGTGATGGCT TCCATGTCGG CAGAATGCTT AAATGAATTA CAAACAGTAC 901 TGCGATGAGT GGCAGGGCGG GGCGTAAACG CGTGGATCCG GGCTTACTAA AAGCCAGAAT 961 AACAGTATGC GTATTTGCGC GCTGGATTTT TTGCGGGTAT AAGAATATAT TACTGGATGT 1021 GTATACCCCG AAGTTATGTC AAAAAAGGAG GTATGCTATT GAAGCCAGCG TTATTTACAG 1081 TTGACAGTTG GACAGCGGAC CAGGCTATCA GTTTGGCCTC CAAGGGCATA TATATTGGAA 1141 TGTCCAATAT TCTTCCGGGT TCTGGGTAAG CACCACCATG TGCCAGAAAT TGGCAAACCC 1201 TCCGGATTCC GTACTCTCGG GTGCGC //
Primer: HIVgp_R1 (Pr0132) Region: HIV-1 psi,5'LTR,CMV pro Sequence file: RDB12104_A5D2h.seq
>04386_12104_A5D2_ccdB-C_F_C01_07.ab1 1 CATTCGGCGG TGCATATCGG GGATGAAGCT GGCGCATGAT GACCACCGAT ATGGCCAGTG 61 TGCCGGTCTC CGTTATCGGG GAAGAAGTGG CTGATCTCAG CCACCGCGAA AATGACATCA 121 AAAACGCCAT TAACCTGATG TTCTGGGGAA TATAAATGTC AGGCTCCCTT ATACACAGCC 181 AGTCTGCAGG TCGACCATAG TGACTGGATA TGTTGTGTTT TACAGTATTA TGTAGTCTGT 241 TTTTTATGCA AAATCTAATT TAATATATTG ATATTTATAT CATTTTACGT TTCTCGTTCA 301 GCTTTCTTGT ACAAAGTGGT GATAATTCTG CAGCGGCCGC GGATCCTCTA GAGTTAACAT 361 CGAGGGATCA AGCTTATCGA TAATCAACCT CTGGATTACA AAATTTGTGA AAGATTGACT 421 GGTATTCTTA ACTATGTTGC TCCTTTTACG CTATGTGGAT ACGCTGCTTT AATGCCTTTG 481 TATCATGCTA TTGCTTCCCG TATGGCTTTC ATTTTCTCCT CCTTGTATAA ATCCTGGTTG 541 CTGTCTCTTT ATGAGGAGTT GTGGCCCGTT GTCAGGCAAC GTGGCGTGGT GTGCACTGTG 601 TTTGCTGACG CAACCCCCAC TGGTTGGGGC ATTGCCACCA CCTGTCAGCT CCTTTCCGGG 661 ACTTTCGCTT TCCCCCTCCC TATTGCCACG GCGGAACTCA TCGCCGCCTG CCTTGCCCGC 721 TGCTGGACAG GGGCTCGGCT GTTGGGCACT GACAATTCCG TGGTGTTGTC GGGGAAGCTG 781 ACGTCCTTTC CATGGCTGCT CGCCTGTGTT GCCACCTGGA TTCTGCGCGG GACGTCCTTC 841 TGCTACGTCC CTTCGGCCCT CAATCCAGCG GACCTTCCTT CCCGCGGCCT GCTGCCGGCT 901 CTGCGGCCTC TTCCGCGTCT TCGCCTTCGC CCTCAGACGA GTCGGATCTC CCTTTGGGCC 961 GCCTCCCCGC ATCGATACCG TCGAGACCTA GAAAACATGG AGCAAATCAC AAGTAGCAAT 1021 ACAGCAGCTA CCAAATGCTG ATTGTGCCTG GCTAGAAGCA CAGAGGAGGG AGGAGGTGGG 1081 TTTTCAGTCA CCACCTCAGT ACCTTAGGAT AATGGACCTA CAGCAGGCTG TAGAATTCTT 1141 AGCCACTTTT TTAAAAGGA //
Primer: BGH_rev (Pr0009) Region: 3'LTR,PRE Sequence file: RDB12104_A5D2i.seq
>04386_12104_A5D2_BGHrerv_D01_10.ab1 1 CTTTCGGGGA CCTGCTAGAG ATTTTCCACA CTGACTAAAA GGGTCTGAGG GATCTCTAGT 61 TACCAGAGTC ACACAACAGA CGGGCACACA CTACTTGAAG CACTCAAGGC AAGCTTTATT 121 GAGGCTTAAG CAGTGGGTTC CCTAGTTAGC CAGAGAGCTC CCAGGCTCAG ATCTGGTCTA 181 ACCAGAGAGA CCCAGTACAG TCCGGATGCA GCTCTCGGGC CATGTGATGA AATGCTAGGC 241 GGCTGTCAAA CCTCCACTCT AATACTTCTC TCTCCGGGTC ATCCATCCCA TGCAGGCTCA 301 CAGGGTGTAA CAAGCGGGTG TTCTCTCCTT CATTGGCTTC TTCTACCTTC TCTTGCTCAA 361 CTGGTACTAG CTTGTAGCAC CATCCAAAGG TCAGTGGATA TCTGATCCCT GGCCCTGGTG 421 TGTAGTTCTG CCAATCAGGG AAGTAGCCTT GTGTGTGGTA GATCCACAGA TCAAGGATAT 481 CTTGTCTTCG TTGGGAGTGA ATTAGCCCTT CCAGTCCCCC CTTTTCTTTT AAAAAGTGGC 541 TAAGATCTAC AGCTGCCTTG TAAGTCATTG GTCTTAAAGG TACCTGAGGT GTGACTGGAA 601 AACCCACCTC CTCCTCCTCT TGTGCTTCTA GCCAGGCACA ATCAGCATTG GTAGCTGCTG 661 TATTGCTACT TGTGATTGCT CCATGTTTTT CTAGGTCTCG ACGGTATCGA TGCGGGGAGG 721 CGGCCCAAAG GGAGATCCGA CTCGTCTGAG GGCGAAGGCG AAGACGCGGA AGAGGCCGCA 781 GAGCCGGCAG CAGGCCGCGG GAAGGAAGGT CCGCTGGATT GAGGGCCGAA GGGACGTAGC 841 AGAAGGACGT CCCGCGCAGA ATCCAGGTGG CAACACAGGC GAGCAGCCAT GGAAAGGACG 901 TCAGCTTCCC CGACAACACC ACGGAATTGT CAGTGCCCAA CAGCCGAGCC CCTGTCCAGC 961 AGCGGGCAAG GCAGGCGGCG ATGAGTTCCG CCGTGGCAAT AGGGAGGGGG AAAGCGAAGT 1021 CCCGGGAAGG AGCTGACAGT GGTGGCAATG CCCCAACCCA GTGGGGGGTT GCGTCAGCAA 1081 ACACAGTGCA CACCACCGCC ACGTTTGCCT TGACACCGGA CCACACTCTT CATAAGAGAC 1141 AGCAACCAAG GTATTTATAT ACAAAGGAAA GGA //
Primer: SV40pro_F (Pr0110) Region: SV40pro_ori,Zeo,SV40pA Sequence file: RDB12104_A5D2j.seq
>04386_12104_A5D2_SV40pro_F_E01_13.ab1 1 CCTTGTTCCC TTGGGCATTC AGAAGTAGTG AGGAGGCTTT TTTGGAGGCC TAGCGCTTTT 61 GCAAAAAGCT CCCGGGAGCT TGTATATCCA TTTTCGGATC TGATCAGCAC GTGTTGACAA 121 TTAATCATCG GCATAGTATA TCGGCATAGT ATAATACGAC AAGGTGAGGA ACTAAACCAT 181 GGCCAAGTTG ACCAGTGCCG TTCCGGTGCT CACCGCGCGC GACGTCGCCG GAGCGGTCGA 241 GTTCTGGACC GACCGGCTCG GGTTCTCCCG GGACTTCGTG GAGGACGACT TCGCCGGTGT 301 GGTCCGGGAC GACGTGACCC TGTTCATCAG CGCGGTCCAG GACCAGGTGG TGCCGGACAA 361 CACCCTGGCC TGGGTGTGGG TGCGCGGCCT GGACGAGCTG TACGCCGAGT GGTCGGAGGT 421 CGTGTCCACG AACTTCCGGG ACGCCTCCGG GCCGGCCATG ACCGAGATCG GCGAGCAGCC 481 GTGGGGGCGG GAGTTCGCCC TGCGCGACCC GGCCGGCAAC TGCGTGCACT TCGTGGCCGA 541 GGAGCAGGAC TGACACGTGC TACGAGATTT CGATTCCACC GCCGCCTTCT ATGAAAGGTT 601 GGGCTTCGGA ATCGTTTTCC GGGACGCCGG CTGGATGATC CTCCAGCGCG GGGATCTCAT 661 GCTGGAGTTC TTCGCCCACC CCAACTTGTT TATTGCAGCT TATAATGGTT ACAAATAAAG 721 CAATAGCATC ACAAATTTCA CAAATAAAGC ATTTTTTTCA CTGCATTCTA GTTGTGGTTT 781 GTCCAAACTC ATCAATGTAT CTTATCATGT CTGTATACCG TCGACCTCTA GCTAGAGCTT 841 GGCGTAATCA TGGTCATAGC TGTTTCCTGT GTGAAATTGT TATCCGCTCA CAATTCCACA 901 CAACATACGA GCCGGAAGCA TAAAGTGTAA AGCCTGGGGT GCCTAATGAG TGAGCTAACT 961 CACATTAATT GCGTTGCGCT CACTGCCCGC TTTCCAGTCG GGAAACCTGT CGTGCCAGCT 1021 GCATTAATGA ATCGGGCCAA CGCGCGGGGA GAGGCGGGTT TGCGTATTGG GCGCTCTTCC 1081 CGCTCTTCGC TCCACTGACT CGCTGCGCTT CGCTCGCTTC GGCTGCGGCG AGCCGTATCA 1141 GCTCACTTCA AAGGGCGCGG TTAATTACCG G //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

user_report	Miyakuni, K., Genome-wide analysis of DNA methylation identifies the apoptosis-related gene UQCRH as a tumor suppressor in renal cancer. Mol. Oncol. 16 (3): 732-749 (2022). PMID 34133843.
user_report	Yasukawa, Y., SAA1 is upregulated in gastric cancer-associated fibroblasts possibly by its enhancer activation. Carcinogenesis 42 (2): 180-189 (2021). PMID 33284950.
user_report	Zhou, L., The beta-galactoside alpha2,6-sialyltranferase 1 (ST6GAL1) inhibits the colorectal cancer metastasis by stabilizing intercellular adhesion molecule-1 via sialylation. Cancer Manag. Res. 11: 6185-6199 (2019). PMID 31308754.
user_report	Inoue, S., Uterine adenomyosis is an oligoclonal disorder associated with KRAS mutations. Nat. Commun. 10 (1): 5785 (2019). PMID 31857578.
user_report	Shinohara, M., Tumor necrosis factor-alpha inhibits differentiation of myogenic cells in human urethral rhabdosphincter. Int. J. Urol. 24 (6): 461-467 (2017). PMID 28332237.
user_report	Kubota, S.I., Whole-Body Profiling of Cancer Metastasis with Single-Cell Resolution. Cell Rep. 20 (1): 236-250 (2017). PMID 28683317.
user_report	Noguchi, K., Molecular analysis of keratocystic odontogenic tumor cell lines derived from sporadic and basal cell nevus syndrome patients. Int. J. Oncol. 51 (6): 1731-1738 (2017). PMID 29039452.
user_report	Fuchigami, T., Fibroblasts promote the collective invasion of ameloblastoma tumor cells in a 3D coculture model. FEBS Open Bio. 7 (12): 2000-2007 (2017). PMID 29226086.
user_report	Yachida, S., Genomic Sequencing Identifies ELF3 as a Driver of Ampullary Carcinoma. Cancer Cell pii: S1535-6108 (15) 00476-6 (2016). PMID 26806338.
user_report	Donai, K., Bovine and porcine fibroblasts can be immortalized with intact karyotype by the expression of mutant cyclin dependent kinase 4, cyclin D, and telomerase. J. Biotechnol., 176: 50-57 (2014). PMID 24589663.
user_report	Inagawa, Y., A human cancer xenograft model utilizing normal pancreatic duct epithelial cells conditionally transformed with defined oncogenes. Carcinogenesis 35 (8): 1840-1846 (2014). PMID 24858378.
user_report	Muraoka, N., MiR-133 promotes cardiac reprogramming by directly repressing Snai1 and silencing fibroblast signatures. EMBO J., 33 (14): 1565-1581 (2014). PMID 24920580.
user_report	Kibe, T., A novel ameloblastoma cell line (AM-3) secretes MMP-9 in response to Wnt-3a and induces osteoclastogenesis. Oral Surg. Oral Med. Oral Pathol. Oral Radiol., 115 (6): 780-788 (2013). PMID 23706918.
user_report	Moriyama, H., Tightly regulated and homogeneous transgene expression in human adipose-derived mesenchymal stem cells by lentivirus with tet-off system. PLoS One, 8 (6): e66274 (2013). PMID 23776652.
user_report	Sasaki, R., Oncogenic transformation of human ovarian surface epithelial cells with defined cellular oncogenes. Carcinogenesis, 30 (3): 423-431 (2012). PMID 19126650.
user_report	Egawa, N., The E1 protein of human papillomavirus type 16 is dispensable for maintenance replication of the viral genome. J. Virol., 86 (6): 3276-3283 (2012). PMID 22238312.
user_report	Kibe, T., Immortalization and characterization of normal oral epithelial cells without using HPV and SV40 genes. Oral Science Internatl., 8 (1): 20-28, 2011.
user_report	Harao M., HLA-A2-restricted CTL epitopes of a novel lung cancer-associated cancer testis antigen, cell division cycle associated 1, can induce tumor-reactive CTL. Int. J. Cancer., 123, 2616-2625 (2008). PMID 18770861.
user_report	Imai K., Identification of a novel tumor-associated antigen, cadherin 3/P-cadherin, as a possible target for immunotherapy of pancreatic, gastric, and colorectal cancers. Clin. Cancer. Res., 14, 6487-6495 (2008). PMID 18927288.
user_report	Irie A., Protein kinase D2 contributes to either IL-2 promoter regulation or induction of cell death upon TCR stimulation depending on its activity in Jurkat cells. Int. Immunol., 18, 1737-1747 (2006). PMID 17077180.

2023.05.22

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