Resource data sheet
DNA Bank Top

CSII-CMV-RfA (#RDB04386)

Backbone vector plasmid of lentivirus construct by Gateway(R) cloning with CMV promoter.


Drawn by SnapGene® software
Sequence (full) RDB04386hts01.seq
Remarks, protocol and/or map (pdf) RDB04386.pdf
Publication Unpublished bioresource
Test sheet RDB12104_A9Cbp1-2.pdf 
Assembled from experimentally sequenced data.
 
Clone info. Backbone vector plasmid of lentivirus construct by Gateway(R) cloning with CMV promoter.
Comment Commonly requested with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394).
Vector backbone CSII-CMV-RfA (Plasmid)
Selectable markers Ampicillin, Chloramphenicol, ccdB (E. coli). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Growth conditions 37C, LB+Amp
Growth remarks Use DB3.1, ccdB Survival or equivalent to amplify this clone. 30 degC is fine.
Depositor|Developer Miyoshi, Hiroyuki |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose)[Word] [Example of MTA ]
Please visit Ordering instruction.[link] 
Terms and conditions for distribution The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer.
Remarks Remember that you will be working with samples containing infectious virus.
Use Survival2 (Invitrogen) or equivalent to amplify this clone.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利機関による非営利学術研究用)[Word] [MTAの記入例 ]
遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。
手続きの概要は、「レンチウイルスベクターの提供申し込み」をご覧ください。
MTAに書く使用条件 本件研究材料は、非営利機関の非営利学術研究に限って提供する。本件研究材料を利用した研究結果等を発表する際は、本件研究材料が故三好浩之博士により開発されたことを明示する。
備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。
このクローンの増殖にはSurvival2 (Invitrogen)あるいは同等の宿主を使用してください。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB04386 CSII-CMV-RfA DNA solution

Ordering instruction of plasmids [in Japanese] [in English]

How to cite this biological resource

Materials & Methods section:

The CSII-CMV-RfA was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB04386).

Reference section:

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

user_report Sakai, M., Genome-scale CRISPR-Cas9 screen identifies host factors as potential therapeutic targets for SARS-CoV-2 infection. iScience 27 (8): 110475 (2024). PMID 39100693. [link to RRC of NBRP]
user_report Sakai, M., Genome-scale CRISPR-Cas9 screen identifies novel host factors as potential therapeutic targets for SARS-CoV-2 infection. bioRxiv doi: 10.1101/2023.03.06.531431 (2023).
user_report Miyakuni, K., Genome-wide analysis of DNA methylation identifies the apoptosis-related gene UQCRH as a tumor suppressor in renal cancer. Mol. Oncol. 16 (3): 732-749 (2022). PMID 34133843. [link to RRC of NBRP]
user_report Yasukawa, Y., SAA1 is upregulated in gastric cancer-associated fibroblasts possibly by its enhancer activation. Carcinogenesis 42 (2): 180-189 (2021). PMID 33284950. [link to RRC of NBRP]
user_report Zhou, L., The beta-galactoside alpha2,6-sialyltranferase 1 (ST6GAL1) inhibits the colorectal cancer metastasis by stabilizing intercellular adhesion molecule-1 via sialylation. Cancer Manag. Res. 11: 6185-6199 (2019). PMID 31308754. [link to RRC of NBRP]
user_report Inoue, S., Uterine adenomyosis is an oligoclonal disorder associated with KRAS mutations. Nat. Commun. 10 (1): 5785 (2019). PMID 31857578. [link to RRC of NBRP]
user_report Shinohara, M., Tumor necrosis factor-alpha inhibits differentiation of myogenic cells in human urethral rhabdosphincter. Int. J. Urol. 24 (6): 461-467 (2017). PMID 28332237. [link to RRC of NBRP]
user_report Kubota, S.I., Whole-Body Profiling of Cancer Metastasis with Single-Cell Resolution. Cell Rep. 20 (1): 236-250 (2017). PMID 28683317. [link to RRC of NBRP]
user_report Noguchi, K., Molecular analysis of keratocystic odontogenic tumor cell lines derived from sporadic and basal cell nevus syndrome patients. Int. J. Oncol. 51 (6): 1731-1738 (2017). PMID 29039452. [link to RRC of NBRP]
user_report Fuchigami, T., Fibroblasts promote the collective invasion of ameloblastoma tumor cells in a 3D coculture model. FEBS Open Bio. 7 (12): 2000-2007 (2017). PMID 29226086. [link to RRC of NBRP]
user_report Yachida, S., Genomic Sequencing Identifies ELF3 as a Driver of Ampullary Carcinoma. Cancer Cell pii: S1535-6108 (15) 00476-6 (2016). PMID 26806338. [link to RRC of NBRP]
user_report Donai, K., Bovine and porcine fibroblasts can be immortalized with intact karyotype by the expression of mutant cyclin dependent kinase 4, cyclin D, and telomerase. J. Biotechnol., 176: 50-57 (2014). PMID 24589663. [link to RRC of NBRP]
user_report Inagawa, Y., A human cancer xenograft model utilizing normal pancreatic duct epithelial cells conditionally transformed with defined oncogenes. Carcinogenesis 35 (8): 1840-1846 (2014). PMID 24858378. [link to RRC of NBRP]
user_report Muraoka, N., MiR-133 promotes cardiac reprogramming by directly repressing Snai1 and silencing fibroblast signatures. EMBO J., 33 (14): 1565-1581 (2014). PMID 24920580. [link to RRC of NBRP]
user_report Kibe, T., A novel ameloblastoma cell line (AM-3) secretes MMP-9 in response to Wnt-3a and induces osteoclastogenesis. Oral Surg. Oral Med. Oral Pathol. Oral Radiol., 115 (6): 780-788 (2013). PMID 23706918. [link to RRC of NBRP]
user_report Moriyama, H., Tightly regulated and homogeneous transgene expression in human adipose-derived mesenchymal stem cells by lentivirus with tet-off system. PLoS One, 8 (6): e66274 (2013). PMID 23776652. [link to RRC of NBRP]
user_report Sasaki, R., Oncogenic transformation of human ovarian surface epithelial cells with defined cellular oncogenes. Carcinogenesis, 30 (3): 423-431 (2012). PMID 19126650. [link to RRC of NBRP]
user_report Egawa, N., The E1 protein of human papillomavirus type 16 is dispensable for maintenance replication of the viral genome. J. Virol., 86 (6): 3276-3283 (2012). PMID 22238312. [link to RRC of NBRP]
user_report Kibe, T., Immortalization and characterization of normal oral epithelial cells without using HPV and SV40 genes. Oral Science Internatl., 8 (1): 20-28, 2011. [link to RRC of NBRP]
user_report Harao M., HLA-A2-restricted CTL epitopes of a novel lung cancer-associated cancer testis antigen, cell division cycle associated 1, can induce tumor-reactive CTL. Int. J. Cancer., 123, 2616-2625 (2008). PMID 18770861. [link to RRC of NBRP]
user_report Imai K., Identification of a novel tumor-associated antigen, cadherin 3/P-cadherin, as a possible target for immunotherapy of pancreatic, gastric, and colorectal cancers. Clin. Cancer. Res., 14, 6487-6495 (2008). PMID 18927288. [link to RRC of NBRP]
user_report Irie A., Protein kinase D2 contributes to either IL-2 promoter regulation or induction of cell death upon TCR stimulation depending on its activity in Jurkat cells. Int. Immunol., 18, 1737-1747 (2006). PMID 17077180. [link to RRC of NBRP]