Resource data sheet
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CSII-EF-MCS-IRES2-Venus

Backbone vector plasmid of lentivirus construct driven by EF-1 alpha promoter with Venus fluorescent protein marker.

Catalog number RDB04384
Resource name CSII-EF-MCS-IRES2-Venus
Clone info. Backbone vector plasmid of lentivirus construct driven by EF-1 alpha promoter with Venus fluorescent protein marker.
Commonly requested with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Vector backbone CSII-EF-MCS-IRES2-Venus (Plasmid)
Selectable markers Please note that Zeo resistance marker is not included in the lentivirus produced from this vector. E. coli: Amp^r.
Gene/insert name Aequorea victoria GFP cDNA
Depositor|Developer Miyoshi, Hiroyuki |
Lentiviral plasmid list, maps and sequences information. [link]

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer.
Additional terms and conditions for distribution RECIPIENT is requested to obtain written consent of Dr. Atsushi Miyawaki of the RIKEN CBS regarding Venus fluorescent protein.
Ordering Please visit Ordering instruction.[link] 
Order form 
[Credit Card Payment]  [Bank Transfer or Check Payment]
Material Transfer Agreement (MTA, for use for not-for-profit academic purpose)[Word]
Approval form [Word] by Dr. Atsushi Miyawaki
Remarks ((Remarks)) Remember that you will be working with samples containing infectious virus.
Please contact us. We will provide you with appropriate documents.
Please obtain written approval of Dr. Atsushi Miyawaki.
Distribution information (domestic users) [open/close]


Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB04384 CSII-EF-MCS-IRES2-Venus Under QC test. Please contact us. DNA solution

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.
Ordering instruction of plasmids [in Japanese] [in English]

References and tips

Electronic file

Electronic file Sequence RDB04384z.seq
Electronic file Remarks, protocol and/or map (pdf) RDB04384.pdf

Original reference

original Hayashi, S., Alternative role of HuD splicing variants in neuronal differentiation. J. Neurosci. Res. 93 (3): 399-409 (2015). PMID 25332105.

Further references such as user reports and related articles (go to bottom)

Featured content


Sequence information

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.


References

Original, user report and related articles

original Hayashi, S., Alternative role of HuD splicing variants in neuronal differentiation. J. Neurosci. Res. 93 (3): 399-409 (2015). PMID 25332105.
user report Morita, K., Allosteric Activators of Protein Phosphatase 2A Display Broad Antitumor Activity Mediated by Dephosphorylation of MYBL2. Cell 181 (3): 702-715.e20 (2020). PMID 32315619.
user report Sugimoto, K., Cell adhesion signals regulate the nuclear receptor activity. Proc. Natl. Acad. Sci. U.S.A. 116 (49): 24600-24609 (2019). PMID 31740618.
user report Takahashi, Y., A Refined Culture System for Human Induced Pluripotent Stem Cell-Derived Intestinal Epithelial Organoids. Stem Cell Reports 10 (1): 314-328 (2018). PMID 29233552.
user report Yoshitomi, H., Human Sox4 facilitates the development of CXCL13-producing helper T cells in inflammatory environments. Nat. Commun. 9 (1): 3762 (2018). PMID 30232328.
user report Hanawa, M., Hepatocyte Nuclear Factor 4 Alpha Promotes Definitive Endoderm Differentiation from Human Induced Pluripotent Stem Cells. Stem Cell Rev. 13 (4): 542-551 (2017). PMID 28000155.
user report Ueno, N., PU.1 acts as tumor suppressor for myeloma cells through direct transcriptional repression of IRF4. Oncogene 36 (31): 4481-4497 (2017). PMID 28368411.
user report Shichita, T., MAFB prevents excess inflammation after ischemic stroke by accelerating clearance of damage signals through MSR1. Nat. Med. 23 (6): 723-732 (2017). PMID 28394332.
user report Morita, K., Genetic regulation of the RUNX transcription factor family has antitumor effects. J. Clin. Invest. 127 (7): 2815-2828 (2017). PMID 28530640.
user report Takahashi, Y., Reciprocal Inflammatory Signaling Between Intestinal Epithelial Cells and Adipocytes in the Absence of Immune Cells. EBioMedicine. 23: 34-45 (2017). PMID 28789943.
user report Morita, K., Autonomous feedback loop of RUNX1-p53-CBFB in acute myeloid leukemia cells. Sci. Rep. 7 (1): 16604 (2017). PMID 29192243.
user report Morita, K., Paradoxical enhancement of leukemogenesis in acute myeloid leukemia with moderately attenuated RUNX1 expressions. Blood Advances 1: 1440-1451 (2017). PMID 29296785.
user report Kobayashi, S., TGF-beta induces the differentiation of human CXCL13-producing CD4(+) T cells. Eur. J. Immunol. 46 (2): 360-371 (2016). PMID 26541894.
user report Fujikawa, D., Polycomb-dependent epigenetic landscape in adult T-cell leukemia. Blood 127 (14): 1790-1802 (2016). PMID 26773042.
user report Kanatsu-Shinohara, M., Nonrandom Germline Transmission of Mouse Spermatogonial Stem Cells. Dev. Cell 38 (3): 248-261 (2016). PMID 27505415.
user report Omura, H., Structural and Functional Analysis of DDX41: a bispecific immune receptor for DNA and cyclic dinucleotide. Sci. Rep. 6: 34756 (2016). PMID 27721487.
user report Matsuda, Y., Epigenetic Heterogeneity in HIV-1 Latency Establishment. Sci. Rep. 5: 7701 (2015). PMID 25572573.
user report Nakatani, T., Stella preserves maternal chromosome integrity by inhibiting 5hmC-induced gammaH2AX accumulation. EMBO Rep. 16 (5): 582-9 (2015). PMID 25694116.
user report Katsuhara, A., Transduction of a novel HLA-DRB1*04:05-restricted, WT1-specific TCR gene into human CD4+ T cells confers killing activity against human leukemia cells. Anticancer Res. 35 (3): 1251-1261 (2015). PMID 25750272.
user report Ito, M., Bruton's tyrosine kinase is essential for NLRP3 inflammasome activation and contributes to ischaemic brain injury. Nat. Commun. 6: 7360 (2015). PMID 26059659.
user report Yu, F.X., Construction of a lentiviral T/A vector for direct analysis of PCR-amplified promoters. Mol. Biol. Rep. 41 (11): 7651-7658 (2014). PMID 25091945.
user report Takayama, G., Class I PI3K-mediated Akt and ERK signals play a critical role in Fc{varepsilon}RI-induced degranulation in mast cells. Int. Immunol., 25 (4): 215-220 (2013). PMID 23143475.
user report Hasegawa, K., Retinoic acid signaling in Sertoli cells regulates organization of the blood-testis barrier through cyclical changes in gene expression. Development, 139 (23): 4347-4355 (2012). PMID 23095883.
user report Nishikawa, E., miR-375 is activated by ASH1 and inhibits YAP1 in a lineage dependent manner in lung cancer. Cancer Res., 71 (19): 6165-6173 (2011). PMID 21856745.
user report Yi Y., Mucin 21/epiglycanin modulates cell adhesion. J. Biol. Chem., 285, 21233-21240 (2010). PMID 20388707.
user report Honda A., Generation of induced pluripotent stem cells in rabbits: potential experimental models for human regenerative medicine. J. Biol. Chem., 285, 31362-31369 (2010). PMID 20670936.
user report Ohoka, N., The orphan nuclear receptor RORalpha restrains adipocyte differentiation through a reduction of C/EBPbeta activity and perilipin gene expression. Mol. Endocrinol., 23, 759-771 (2009). PMID 19324970.
user report Lee, J., Genetic reconstruction of mouse spermatogonial stem cell self-renewal in vitro by Ras-cyclin D2 activation. Cell Stem. Cell, 5, 76-86 (2009). PMID 19570516.
user report Miura K., Variation in the safety of induced pluripotent stem cell lines. Nat. Biotechnol., 27, 743-745 (2009). PMID 19590502.
user report Naka, H., Requirement for COUP-TFI and II in the temporal specification of neural stem cells in CNS development. Nat. Neurosci. 11 (9): 1014-1023 (2008). PMID 19160499.

2020.12.24

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