CSII-EF-MCS-IRES2-Venus (#RDB04384)

Backbone vector plasmid of lentivirus construct driven by EF-1 alpha promoter with Venus fluorescent protein marker.

Drawn by SnapGene® software

Sequence (full) RDB04384hts01.seq Remarks, protocol and/or map (pdf) RDB04384.pdf Publication Hayashi, S., J. Neurosci. Res. 93 (3): 399-409 (2015). [link to RRC of NBRP] Test sheet RDB18954_B1F2p1-2.pdf  Assembled from experimentally sequenced data.

 

Clone info.	Backbone vector plasmid of lentivirus construct driven by EF-1 alpha promoter with Venus fluorescent protein marker.
Comment	Commonly requested with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394).
Vector backbone	CSII-EF-MCS-IRES2-Venus (RDB04378) (Plasmid)
Selectable markers	Ampicillin (E. coli). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Gene/insert name	Aequorea victoria GFP cDNA
Depositor|Developer	Miyoshi, Hiroyuki |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Ordering instruction.[link]
Terms and conditions for distribution	The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer.
Remarks	((Remarks)) Remember that you will be working with samples containing infectious virus. Please contact us. We will provide you with appropriate documents.

提供案内 (日本国内) [open/close]

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB04384	CSII-EF-MCS-IRES2-Venus	DNA solution

Ordering instruction of plasmids [in Japanese] [in English]

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How to cite this biological resource

Materials & Methods section:

The CSII-EF-MCS-IRES2-Venus was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB04384).

Reference section:

Hayashi, S., Yano, M., Igarashi, M., Okano, H.J., Okano, H., Alternative role of HuD splicing variants in neuronal differentiation. J. Neurosci. Res. 93 (3): 399-409 (2015). PMID 25332105. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

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References

Original, user report and related articles

original	Hayashi, S., Alternative role of HuD splicing variants in neuronal differentiation. J. Neurosci. Res. 93 (3): 399-409 (2015). PMID 25332105. [link to RRC of NBRP]
reference	Seita, Y., Generation of transgenic cynomolgus monkeys that express green fluorescent protein throughout the whole body. Sci. Rep. 6: 24868 (2016). PMID 27109065.
user_report	Yamagishi, M., Mechanisms of action and resistance in histone methylation-targeted therapy. Nature 627 (8002): 221-228 (2024). PMID 38383791. [link to RRC of NBRP]
user_report	Zhang, H., The maturation of iPS cell-derived brain microvascular endothelial cells by inducible-SOX18 expression. Fluids Barriers CNS 20 (1): 10 (2023). PMID 36732767. [link to RRC of NBRP]
user_report	Fujiki, F., Establishment of a novel NFAT-GFP reporter platform useful for the functional avidity maturation of HLA class II-restricted TCRs. Cancer Immunol. Immunother. 72 (7): 2347-2356 (2023). PMID 36939853. [link to RRC of NBRP]
user_report	Sakai, E., miR-27b targets MAIP1 to mediate lipid accumulation in cultured human and mouse hepatic cells. Commun. Biol. 6 (1): 669 (2023). PMID 37355744. [link to RRC of NBRP]
user_report	Okada, M., Detection of mutant antigen-specific T cell receptors against multiple myeloma for T cell engineering. Mol. Ther. Methods. Clin. Dev. 29: 541-555 (2023). PMID 37359417. [link to RRC of NBRP]
user_report	Kano, M., Functional calcium-responsive parathyroid glands generated using single-step blastocyst complementation. Proc. Natl. Acad. Sci. U.S.A. 120 (28): e2216564120 (2023). PMID 37379351. [link to RRC of NBRP]
user_report	Oiwa, K., Monomerization of TDP-43 is a key determinant for inducing TDP-43 pathology in amyotrophic lateral sclerosis. Sci Adv. 2023 Aug 4;9(31):eadf6895 (2023). PMID 37540751. [link to RRC of NBRP]
user_report	Konaka, H., Secretion of mitochondrial DNA via exosomes promotes inflammation in Behçet's syndrome. EMBO J. 42 (20): e112573 (2023). PMID 37661814. [link to RRC of NBRP]
user_report	Yamaguchi, T., Development and Functional Evaluation of MDR1-expressing Microvascular Endothelial-like Cells Derived from Human iPS Cells as an In vitro Blood-brain Barrier Model. J. Pharm. Sci. 112 (12): 3216-3223 (2023). PMID 37690777. [link to RRC of NBRP]
user_report	Matsumoto, N., Replication of Human Sapovirus in Human-Induced Pluripotent Stem Cell-Derived Intestinal Epithelial Cells. Viruses 15 (9): 1929 (2023). PMID 37766335. [link to RRC of NBRP]
user_report	Kuboyama-Sasaki, A., Establishment of a cell culture platform for human liver organoids and its application for lipid metabolism research. Biotechnol. J. 2: e2300365 (2023). PMID 37920068. [link to RRC of NBRP]
user_report	Zhang, H., Transient ETV2 Expression Promotes the Generation of Mature Endothelial Cells from Human Pluripotent Stem Cells. Biol. Pharm. Bull. 45 (4): 483-490 (2022). PMID 35370273. [link to RRC of NBRP]
user_report	Kanatsu-Shinohara, M., Adeno-associated-virus-mediated gene delivery to ovaries restores fertility in congenital infertile mice. Cell Rep. Med. 3 (5): 100606 (2022) PMID 35584625. [link to RRC of NBRP]
user_report	Fujiki, F., T Cell-Intrinsic Vitamin A Metabolism and Its Signaling Are Targets for Memory T Cell-Based Cancer Immunotherapy. Front. Immunol. 13: 935465 (2022). PMID 35844620. [link to RRC of NBRP]
user_report	Song, W., E-cadherin maintains the undifferentiated state of mouse spermatogonial progenitor cells via β-catenin. Cell Biosci. 12 (1): 141 (2022). PMID 36050783. [link to RRC of NBRP]
user_report	Endo, Y., Claudin‑9 is a novel prognostic biomarker for endometrial cancer. Int. J. Oncol. 61 (5): 135 (2022). PMID 36129146. [link to RRC of NBRP]
user_report	Takahashi, H., MYPT1-PP1β phosphatase negatively regulates both chromatin landscape and co-activator recruitment for beige adipogenesis. Nat. Commun. 13 (1): 5715 (2022). PMID 36175407. [link to RRC of NBRP]
user_report	Jin, M., DOPAnization of tyrosine in α-synuclein by tyrosine hydroxylase leads to the formation of oligomers. Nat. Commun. 13 (1): 6880 (2022). PMID 36371400. [link to RRC of NBRP]
user_report	Yamagishi, M., Chronological genome and single-cell transcriptome integration characterizes the evolutionary process of adult T cell leukemia-lymphoma. Nat. Commun. 12 (1): 4821 (2021). PMID 34376672. [link to RRC of NBRP]
user_report	Mizuno, T., Development of a cell line-based assay to measure the antibody-dependent cellular cytotoxicity of a canine therapeutic antibody. Vet. Immunol. Immunopathol. 240: 110315 (2021). PMID 34450340. [link to RRC of NBRP]
user_report	Wang, L.Y., Sequestration of RBM10 in Nuclear Bodies: Targeting Sequences and Biological Significance. Int, J, Mol, Sci. 22 (19): 10526 (2021). PMID 34638866. [link to RRC of NBRP]
user_report	Ji, W.H., Cytochrome P450 26A1 Modulates the Polarization of Uterine Macrophages During the Peri-Implantation Period. Front. Immunol. 12: 763067 (2021). PMID 34712245. [link to RRC of NBRP]
user_report	Morita, K., Allosteric Activators of Protein Phosphatase 2A Display Broad Antitumor Activity Mediated by Dephosphorylation of MYBL2. Cell 181 (3): 702-715.e20 (2020). PMID 32315619. [link to RRC of NBRP]
user_report	Teramae, A., The Molecular Basis of Chemical Chaperone Therapy for Oculocutaneous Albinism Type 1A. J. Invest. Dermatol. 139 (5): 1143-1149 (2019). PMID 30447237. [link to RRC of NBRP]
user_report	Sugimoto, K., Cell adhesion signals regulate the nuclear receptor activity. Proc. Natl. Acad. Sci. U.S.A. 116 (49): 24600-24609 (2019). PMID 31740618. [link to RRC of NBRP]
user_report	Takahashi, Y., A Refined Culture System for Human Induced Pluripotent Stem Cell-Derived Intestinal Epithelial Organoids. Stem Cell Reports 10 (1): 314-328 (2018). PMID 29233552. [link to RRC of NBRP]
user_report	Yoshitomi, H., Human Sox4 facilitates the development of CXCL13-producing helper T cells in inflammatory environments. Nat. Commun. 9 (1): 3762 (2018). PMID 30232328. [link to RRC of NBRP]
user_report	Hanawa, M., Hepatocyte Nuclear Factor 4 Alpha Promotes Definitive Endoderm Differentiation from Human Induced Pluripotent Stem Cells. Stem Cell Rev. 13 (4): 542-551 (2017). PMID 28000155. [link to RRC of NBRP]
user_report	Ueno, N., PU.1 acts as tumor suppressor for myeloma cells through direct transcriptional repression of IRF4. Oncogene 36 (31): 4481-4497 (2017). PMID 28368411. [link to RRC of NBRP]
user_report	Shichita, T., MAFB prevents excess inflammation after ischemic stroke by accelerating clearance of damage signals through MSR1. Nat. Med. 23 (6): 723-732 (2017). PMID 28394332. [link to RRC of NBRP]
user_report	Morita, K., Genetic regulation of the RUNX transcription factor family has antitumor effects. J. Clin. Invest. 127 (7): 2815-2828 (2017). PMID 28530640. [link to RRC of NBRP]
user_report	Takahashi, Y., Reciprocal Inflammatory Signaling Between Intestinal Epithelial Cells and Adipocytes in the Absence of Immune Cells. EBioMedicine. 23: 34-45 (2017). PMID 28789943. [link to RRC of NBRP]
user_report	Morita, K., Autonomous feedback loop of RUNX1-p53-CBFB in acute myeloid leukemia cells. Sci. Rep. 7 (1): 16604 (2017). PMID 29192243. [link to RRC of NBRP]
user_report	Morita, K., Paradoxical enhancement of leukemogenesis in acute myeloid leukemia with moderately attenuated RUNX1 expressions. Blood Adv. 1: 1440-1451 (2017). PMID 29296785. [link to RRC of NBRP]
user_report	Kobayashi, S., TGF-beta induces the differentiation of human CXCL13-producing CD4(+) T cells. Eur. J. Immunol. 46 (2): 360-371 (2016). PMID 26541894. [link to RRC of NBRP]
user_report	Fujikawa, D., Polycomb-dependent epigenetic landscape in adult T-cell leukemia. Blood 127 (14): 1790-1802 (2016). PMID 26773042. [link to RRC of NBRP]
user_report	Takahashi, Y., Perilipin2 plays a positive role in adipocytes during lipolysis by escaping proteasomal degradation. Sci Rep. 6: 20975 (2016). PMID 26876687. [link to RRC of NBRP]
user_report	Kanatsu-Shinohara, M., Nonrandom Germline Transmission of Mouse Spermatogonial Stem Cells. Dev. Cell 38 (3): 248-261 (2016). PMID 27505415. [link to RRC of NBRP]
user_report	Omura, H., Structural and Functional Analysis of DDX41: a bispecific immune receptor for DNA and cyclic dinucleotide. Sci. Rep. 6: 34756 (2016). PMID 27721487. [link to RRC of NBRP]
user_report	Matsuda, Y., Epigenetic Heterogeneity in HIV-1 Latency Establishment. Sci. Rep. 5: 7701 (2015). PMID 25572573. [link to RRC of NBRP]
user_report	Nakatani, T., Stella preserves maternal chromosome integrity by inhibiting 5hmC-induced gammaH2AX accumulation. EMBO Rep. 16 (5): 582-9 (2015). PMID 25694116. [link to RRC of NBRP]
user_report	Katsuhara, A., Transduction of a novel HLA-DRB1*04:05-restricted, WT1-specific TCR gene into human CD4+ T cells confers killing activity against human leukemia cells. Anticancer Res. 35 (3): 1251-1261 (2015). PMID 25750272. [link to RRC of NBRP]
user_report	Ito, M., Bruton's tyrosine kinase is essential for NLRP3 inflammasome activation and contributes to ischaemic brain injury. Nat. Commun. 6: 7360 (2015). PMID 26059659. [link to RRC of NBRP]
user_report	Yu, F.X., Construction of a lentiviral T/A vector for direct analysis of PCR-amplified promoters. Mol. Biol. Rep. 41 (11): 7651-7658 (2014). PMID 25091945. [link to RRC of NBRP]
user_report	Takayama, G., Class I PI3K-mediated Akt and ERK signals play a critical role in Fc{varepsilon}RI-induced degranulation in mast cells. Int. Immunol., 25 (4): 215-220 (2013). PMID 23143475. [link to RRC of NBRP]
user_report	Hasegawa, K., Retinoic acid signaling in Sertoli cells regulates organization of the blood-testis barrier through cyclical changes in gene expression. Development, 139 (23): 4347-4355 (2012). PMID 23095883. [link to RRC of NBRP]
user_report	Nishikawa, E., miR-375 is activated by ASH1 and inhibits YAP1 in a lineage dependent manner in lung cancer. Cancer Res., 71 (19): 6165-6173 (2011). PMID 21856745. [link to RRC of NBRP]
user_report	Yi Y., Mucin 21/epiglycanin modulates cell adhesion. J. Biol. Chem., 285, 21233-21240 (2010). PMID 20388707. [link to RRC of NBRP]
user_report	Honda A., Generation of induced pluripotent stem cells in rabbits: potential experimental models for human regenerative medicine. J. Biol. Chem., 285, 31362-31369 (2010). PMID 20670936. [link to RRC of NBRP]
user_report	Ohoka, N., The orphan nuclear receptor RORalpha restrains adipocyte differentiation through a reduction of C/EBPbeta activity and perilipin gene expression. Mol. Endocrinol., 23, 759-771 (2009). PMID 19324970. [link to RRC of NBRP]
user_report	Lee, J., Genetic reconstruction of mouse spermatogonial stem cell self-renewal in vitro by Ras-cyclin D2 activation. Cell Stem. Cell, 5, 76-86 (2009). PMID 19570516. [link to RRC of NBRP]
user_report	Miura K., Variation in the safety of induced pluripotent stem cell lines. Nat. Biotechnol., 27, 743-745 (2009). PMID 19590502. [link to RRC of NBRP]
user_report	Naka, H., Requirement for COUP-TFI and II in the temporal specification of neural stem cells in CNS development. Nat. Neurosci. 11 (9): 1014-1023 (2008). PMID 19160499. [link to RRC of NBRP]
user_report	Fumoto, T., Orphan nuclear receptor Nur77 accelerates the initial phase of adipocyte differentiation in 3T3-L1 cells by promoting mitotic clonal expansion. J. Biochem. 141 (2): 181-192 (2007). PMID 17167038. [link to RRC of NBRP]

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Aequorea victoria GFP

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