Resource data sheet
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CSII-EF-MCS-IRES2-Venus

Backbone vector of lentivirus construct driven by EF-1 alpha promoter with Venus fluorescent protein marker.

Catalog number RDB04384
Resource name CSII-EF-MCS-IRES2-Venus
Clone info. Backbone vector of lentivirus construct driven by EF-1 alpha promoter with Venus fluorescent protein marker.
Commonly requested with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Vector backbone CSII-EF-MCS (Plasmid)
Selectable markers Amp^r (E. coli); Zeo^r (plasmid transfection of mammalian cells).
Gene/insert name Aequorea victoria GFP cDNA
Originator Miyoshi, Hiroyuki |
CLICK here for plasmid list. [link]

Distribution information

Terms and conditions for distribution The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe Dr. Hiroyuki Miyoshi (The Developer, the current address: Keio University) as the Developer. Dr. Miyoshi may request the RECIPIENT to report on progress and/or results obtained through the use of the BIOLOGICAL RESOURCE.
Ordering Please visit Ordering instruction. 
Order form 
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Exclusive MTA [Word]
Approval form [Word] by Dr. Atsushi Miyawaki
Remarks ((Remarks)) Remember that you will be working with samples containing infectious virus.
Please contact us. We will provide you with appropriate documents.
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB04384 CSII-EF-MCS-IRES2-Venus DNA solution

Ordering instruction of plasmids [in Japanese] [in English]

References and tips

Electronic file

Electronic file Sequence RDB04384z.seq from Depositor
Electronic file PDF RDB04384.pdf from Depositor

Featured content

References

original Hayashi, S., Alternative role of HuD splicing variants in neuronal differentiation. J. Neurosci. Res. 93 (3): 399-409 (2015). PMID 25332105.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB13022_A4L1p1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: IRES-R RDB13022_A4L1a.seq
>04384_13022_A4L1_IRES-R_A01_01.ab1
    1 CCATTAACGG CCTTCGGCAG TAACGTTAGG GGGGGGGGAG GGAGAGGGGC GGATCCGCGG
   61 CCGCTGCAGA ATTCTCGAGA CCGGTAGCGT GTTCACGACA CCTGAAATGG AAGAAAAAAA
  121 CTTTGAACCA CTGTCTGAGG CTTGAGAATG AACCAAGATC CAAACTCAAA AAGGGCAAAT
  181 TCCAAGGAGA ATTACATCAA GTGCCAAGCT GGCCTAACTT CAGTCTCCAC CCACTCAGTG
  241 TGGGGAAACT CCATCGCATA AAACCCCTCC CCCCAACCTA AAGACGACGT ACTCCAAAAG
  301 CTCCAGAACT AATCGAGGTG CCTGGACGGC GCCCGGTACT CCGTGGAGTC ACATGAAGCG
  361 ACGGCTGAGG ACGGAAAGGC CCCTTTCCTT TGTGTGGGTG ACTCACCCGC CCGCTCTCCC
  421 GAGCGCCGCG TCCTCCATTT TGAGCCCCCT GGAGCAGGGC CGGGAAGCGG CCATCTTTCC
  481 GCTCACGCAA CTGGTGCCGA CCGGGCCAGC CTTGCCGCCC AGGGCGGGGC GATACACGGC
  541 GGCGCGAGGC CAGGCACCAG AGCAGGCCGG CCAGCTTGAG ACTACCCCCG TCCGATTCTC
  601 GGTGGCCGCG CTCGCAGGCC CCGCCTCGCC GAACATGTGC GCTGGGACGC ACGGGCCCCG
  661 TCGCCGCCCG CGGCCCCAAA AACCGAAATA CCAGTGTGCA GATCCTGGCC CGCATTTACA
  721 AGACTATCTT GCCAGAAAAA AAGCGTCGCA GCAGGTCATC AAAAATTTTA AATGGCTAGA
  781 GACTTATCGA AAGCAGCGAG ACAGGCGCGA AGGTGCCACC AGATTCGCAC GCGGCGGCCC
  841 CAGCGCCCAG GCCAGGCCTC AACTCAAGCA CGAGGCGAAG GGGCTCCTAA AGCGCAAGGC
  901 CCGCCCCTGG CTCCAGCTCG GGATCAAGAA TCACGTACTG GAGCCAGGTG GAAGTAATTC
  961 AAGGCACGCA AGGGCCATAA CCCGTAAAAG AGGCCAGGCC CGCGGGAACC ACACACGGCA
 1021 CTTACCTGTG TTCTGGCGGC AAACCCGTTG CGAAAAGACG TTTCACGGCG ACTACTGCAC
 1081 TTATATACCG TCTCCCCCCA ACCCTCGGAA AAGCGAGCCA GTACACGACA TCACTTTCCC
1141 AGTTAACCCC GCGCCACTTC CTCTAAGGGG CA
//
Primer: IRES-F RDB13022_A4L1b.seq
>04384_13022_A4L1_IRES-F_B01_04.ab1
    1 TCTGGCCTTT TAATGTGTTT TAGGTCGAGG TTAAAAAAAC GTCTAGGCCC CCCGAACCAC
   61 GGGGACGTGG TTTTCCTTTG AAAAACACGA TGATAATATG GCCACAACCA TGGTGAGCAA
  121 GGGCGAGGAG CTGTTCACCG GGGTGGTGCC CATCCTGGTC GAGCTGGACG GCGACGTAAA
  181 CGGCCACAAG TTCAGCGTGT CCGGCGAGGG CGAGGGCGAT GCCACCTACG GCAAGCTGAC
  241 CCTGAAGCTG ATCTGCACCA CCGGCAAGCT GCCCGTGCCC TGGCCCACCC TCGTGACCAC
  301 CCTGGGCTAC GGCCTGCAGT GCTTCGCCCG CTACCCCGAC CACATGAAGC AGCACGACTT
  361 CTTCAAGTCC GCCATGCCCG AAGGCTACGT CCAGGAGCGC ACCATCTTCT TCAAGGACGA
  421 CGGCAACTAC AAGACCCGCG CCGAGGTGAA GTTCGAGGGC GACACCCTGG TGAACCGCAT
  481 CGAGCTGAAG GGCATCGACT TCAAGGAGGA CGGCAACATC CTGGGGCACA AGCTGGAGTA
  541 CAACTACAAC AGCCACAACG TCTATATCAC CGCCGACAAG CAGAAGAACG GCATCAAGGC
  601 CAACTTCAAG ATCCGCCACA ACATCGAGGA CGGCGGCGTG CAGCTCGCCG ACCACTACCA
  661 GCAGAACACC CCCATCGGCG ACGGCCCCGT GCTGCTGCCC GACAACCACT ACCTGAGCTA
  721 CCAGTCCGCC CTGAGCAAAG ACCCCAACGA GAAGCGCGAT CACATGGTCC TGCTGGAGTT
  781 CGTGACCGCC GCCGGGATCA CTCTCGGCAT GGACGAGCTG TACAAGTAAG AATTCAAGGC
  841 CTCTCGAGCC TCTAGAGTTA ACATCGAGGG ATCAAGCTTA TCGATAATCA ACCTCTGGAT
  901 TACAAAATTT GTGAAAAGAT TGACTGGTAT TCTTAACTAT GTTTGCTCCT TTTTACGCTA
  961 TGTGGGATAC GCTGCTTTAA TGCCTTTGTA TCATGCTATT GCTTTCCCGT ATGGCTTTCA
 1021 TTTTCTCCTC CTTGTAATAA ATCCTGGTTG CTGTCTCTTT ATGAGGAGTT GTGTCCGTTG
 1081 TTCAGGCAAC GTGGCGTGGT GGTGCACTGT GTTGGCCTGA CGCAACCCCC CAACTTGTTG
1141 GGGCCATGGC CCACCTAACC TGTGTCAGCC TCCCCTAT
//
Primer: BGH_rev RDB13022_A4L1c.seq
>04384_13022_A4L1_BGHrev_C01_07.ab1
    1 CGCTACGGGG TCTGCTAGAG ATTTTCCACA CTGACTAAAA GGGTCTGAGG GATCTCTAGT
   61 TACCAGAGTC ACACAACAGA CGGGCACACA CTACTTGAAG CACTCAAGGC AAGCTTTATT
  121 GAGGCTTAAG CAGTGGGTTC CCTAGTTAGC CAGAGAGCTC CCAGGCTCAG ATCTGGTCTA
  181 ACCAGAGAGA CCCAGTACAG TCCGGATGCA GCTCTCGGGC CATGTGATGA AATGCTAGGC
  241 GGCTGTCAAA CCTCCACTCT AATACTTCTC TCTCCGGGTC ATCCATCCCA TGCAGGCTCA
  301 CAGGGTGTAA CAAGCGGGTG TTCTCTCCTT CATTGGCTTC TTCTACCTTC TCTTGCTCAA
  361 CTGGTACTAG CTTGTAGCAC CATCCAAAGG TCAGTGGATA TCTGATCCCT GGCCCTGGTG
  421 TGTAGTTCTG CCAATCAGGG AAGTAGCCTT GTGTGTGGTA GATCCACAGA TCAAGGATAT
  481 CTTGTCTTCG TTGGGAGTGA ATTAGCCCTT CCAGTCCCCC CTTTTCTTTT AAAAAGTGGC
  541 TAAGATCTAC AGCTGCCTTG TAAGTCATTG GTCTTAAAGG TACCTGAGGT GTGACTGGAA
  601 AACCCACCTC CTCCTCCTCT TGTGCTTCTA GCCAGGCACA ATCAGCATTG GTAGCTGCTG
  661 TATTGCTACT TGTGATTGCT CCATGTTTTT CTAGGTCTCG ACGGTATCGA TGCGGGGAGG
  721 CGGCCCAAAG GGAGATCCGA CTCGTCTGAG GGCGAAGGCG AAGACGCGGA AGAGGCCGCA
  781 GAGCCGGCAG CAGGCCGCGG GAAGGAAGGT CCGCTGGATT GAGGGCCGAA GGGACGTAGC
  841 AGAAGGACGT CCCGCGCAGA ATCCAGGTGG CAACACAGGC GAGCAGCCAT GGAAAGGACG
  901 TCAGCTTCCC CGACAACACC ACGGAATTGT CAGTGCCCAA CAGCCGAGCC CCTGTCCAGC
  961 AGCGGGCAAA GGCAGGCGGC GATGAGTTCC GCCGTGGCAA TAGGGAGGGG GAAAGCGAAA
 1021 GTCCCGGGAA AGGAGCTGAC AGTGGTGGCA ATGCCCCAAC CCAGTGGGGG TTGCGTCAGC
 1081 AAACACAGTG CACACACGCA CGTTGCTGAC AACGGACCAC ACTCTCATAA GAGACAGCAC
1141 AGAATTTATA CACGAGGAAG AAAAATGAAA GGCCCAATAG
//
Primer: CMV-Forward RDB13022_A4L1d.seq
>04384_13022_A4L1_CMV-Forward_D01_10.ab1
    1 CTGTACAACC TCCGGCCCCC CTTTGACCGC AAATGGGCGG TAGGCGTTGT ACGGTGGGAG
   61 GTCTATATAA GCAGCGCGTT TTGCCTGTAC TGGGTCTCTC TGGTTAGACC AGATCTGAGC
  121 CTGGGAGCTC TCTGGCTAAC TAGGGAACCC ACTGCTTAAG CCTCAATAAA GCTTGCCTTG
  181 AGTGCTTCAA GTAGTGTGTG CCCGTCTGTT GTGTGACTCT GGTAACTAGA GATCCCTCAG
  241 ACCCTTTTAG TCAGTGTGGA AAATCTCTAG CAGTGGCGCC CGAACAGGGA CTTGAAAGCG
  301 AAAGGGAAAC CAGAGGAGCT CTCTCGACGC AGGACTCGGC TTGCTGAAGC GCGCACGGCA
  361 AGAGGCGAGG GGCGGCGACT GGTGAGTACG CCAAAAATTT TGACTAGCGG AGGCTAGAAG
  421 GAGAGAGATG GGTGCGAGAG CGTCAGTATT AAGCGGGGGA GAATTAGATC GCGATGGGAA
  481 AAAATTCGGT TAAGGCCAGG GGGAAAGAAA AAATATAAAT TAAAACATAT AGTATGGGCA
  541 AGCAGGGAGC TAGAACGATT CGCAGTTAAT CCTGGCCTGT TAGAAACATC AGAAGGCTGT
  601 AGACAAATAC TGGGACAGCT ACAACCATCC CTTCAGACAG GATCAGAAGA ACTTAGATCA
  661 TTATATAATA CAGTAGCAAC CCTCTATTGT GTGCATCAAA GGATAGAGAT AAAAGACACC
  721 AAGGAAGCTT TAGACAAGAT AGAGGAAGAG CAAAACAAAA GTAAGACCAC CGCACAGCAA
  781 GGGCCGCTGA TCTTCAGACC TGGAGGAGGA GATATGAGGG ACAATTGGAG AAGTGAATTA
  841 TATAAATATA AAGTAGTAAA AAATTGAACC ATTAGGAGTT AGCACCCACC AAGGCAAAAG
  901 AGAAGAGTGG TGCAGAGAGA AAAAAAGAGC AGTGGGAATA GGAGCTTTGT TCCTTGGGGT
  961 TCTTGGGAGC AGCAGGAAGC ACTATGGGCG CAGCCGTCAA TGACGCTGAC GGTACAGGCC
 1021 AGACAATTAT TGGTCTGGTA TAGTGGCAGC AGCCAGAACA ATTTGCTGGA GGGCCTAATT
1081 GGAGGGCGCA AACAGCAATC TGGTTGGCAA CC
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Hayashi, S., Alternative role of HuD splicing variants in neuronal differentiation. J. Neurosci. Res. 93 (3): 399-409 (2015). PMID 25332105.
user report Takahashi, Y., A Refined Culture System for Human Induced Pluripotent Stem Cell-Derived Intestinal Epithelial Organoids. Stem Cell Reports 10 (1): 314-328 (2018). PMID 29233552.
user report Hanawa, M., Hepatocyte Nuclear Factor 4 Alpha Promotes Definitive Endoderm Differentiation from Human Induced Pluripotent Stem Cells. Stem Cell Rev. 13 (4): 542-551 (2017). PMID 28000155.
user report Ueno, N., PU.1 acts as tumor suppressor for myeloma cells through direct transcriptional repression of IRF4. Oncogene 36 (31): 4481-4497 (2017). PMID 28368411.
user report Shichita, T., MAFB prevents excess inflammation after ischemic stroke by accelerating clearance of damage signals through MSR1. Nat. Med. 23 (6): 723-732 (2017). PMID 28394332.
user report Morita, K., Genetic regulation of the RUNX transcription factor family has antitumor effects. J. Clin. Invest. 127 (7): 2815-2828 (2017). PMID 28530640.
user report Takahashi, Y., Reciprocal Inflammatory Signaling Between Intestinal Epithelial Cells and Adipocytes in the Absence of Immune Cells. EBioMedicine. 23: 34-45 (2017). PMID 28789943.
user report Morita, K., Autonomous feedback loop of RUNX1-p53-CBFB in acute myeloid leukemia cells. Sci. Rep. 7 (1): 16604 (2017). PMID 29192243.
user report Morita, K., Paradoxical enhancement of leukemogenesis in acute myeloid leukemia with moderately attenuated RUNX1 expressions. Blood Advances 1: 1440-1451 (2017). PMID 29296785.
user report Fujikawa, D., Polycomb-dependent epigenetic landscape in adult T-cell leukemia. Blood 127 (14): 1790-1802 (2016). PMID 26773042.
user report Kanatsu-Shinohara, M., Nonrandom Germline Transmission of Mouse Spermatogonial Stem Cells. Dev. Cell 38 (3): 248-261 (2016). PMID 27505415.
user report Omura, H., Structural and Functional Analysis of DDX41: a bispecific immune receptor for DNA and cyclic dinucleotide. Sci. Rep. 6: 34756 (2016). PMID 27721487.
user report Matsuda, Y., Epigenetic Heterogeneity in HIV-1 Latency Establishment. Sci. Rep. 5: 7701 (2015). PMID 25572573.
user report Nakatani, T., Stella preserves maternal chromosome integrity by inhibiting 5hmC-induced gammaH2AX accumulation. EMBO Rep. 16 (5): 582-9 (2015). PMID 25694116.
user report Katsuhara, A., Transduction of a novel HLA-DRB1*04:05-restricted, WT1-specific TCR gene into human CD4+ T cells confers killing activity against human leukemia cells. Anticancer Res. 35 (3): 1251-1261 (2015). PMID 25750272.
user report Ito, M., Bruton's tyrosine kinase is essential for NLRP3 inflammasome activation and contributes to ischaemic brain injury. Nat. Commun. 6: 7360 (2015). PMID 26059659.
user report Yu, F.X., Construction of a lentiviral T/A vector for direct analysis of PCR-amplified promoters. Mol. Biol. Rep. 41 (11): 7651-7658 (2014). PMID 25091945.
user report Takayama, G., Class I PI3K-mediated Akt and ERK signals play a critical role in Fc{varepsilon}RI-induced degranulation in mast cells. Int. Immunol., 25 (4): 215-220 (2013). PMID 23143475.
user report Hasegawa, K., Retinoic acid signaling in Sertoli cells regulates organization of the blood-testis barrier through cyclical changes in gene expression. Development, 139 (23): 4347-4355 (2012). PMID 23095883.
user report Nishikawa, E., miR-375 is activated by ASH1 and inhibits YAP1 in a lineage dependent manner in lung cancer. Cancer Res., 71 (19): 6165-6173 (2011). PMID 21856745.
user report Yi Y., Mucin 21/epiglycanin modulates cell adhesion. J. Biol. Chem., 285, 21233-21240 (2010). PMID 20388707.
user report Honda A., Generation of induced pluripotent stem cells in rabbits: potential experimental models for human regenerative medicine. J. Biol. Chem., 285, 31362-31369 (2010). PMID 20670936.
user report Ohoka, N., The orphan nuclear receptor RORalpha restrains adipocyte differentiation through a reduction of C/EBPbeta activity and perilipin gene expression. Mol. Endocrinol., 23, 759-771 (2009). PMID 19324970.
user report Lee, J., Genetic reconstruction of mouse spermatogonial stem cell self-renewal in vitro by Ras-cyclin D2 activation. Cell Stem. Cell, 5, 76-86 (2009). PMID 19570516.
user report Miura K., Variation in the safety of induced pluripotent stem cell lines. Nat. Biotechnol., 27, 743-745 (2009). PMID 19590502.
user report Naka, H., Requirement for COUP-TFI and II in the temporal specification of neural stem cells in CNS development. Nat. Neurosci. 11 (9): 1014-1023 (2008). PMID 19160499.

2018.10.22

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