Resource data sheet
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CSII-CMV-MCS-IRES2-Venus

Backbone vector of lentivirus construct driven by CMV promoter with Venus fluorescent protein marker.

Catalog number RDB04383
Resource name CSII-CMV-MCS-IRES2-Venus
Clone info. Backbone vector of lentivirus construct driven by CMV promoter with Venus fluorescent protein marker.
Commonly requested with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Vector backbone CSII-CMV-MCS (Plasmid)
Selectable markers Please note that Zeo resistance marker is not included in the lentivirus produced from this vector. E. coli: Amp^r.
Gene/insert name Aequorea victoria GFP cDNA
Originator Miyoshi, Hiroyuki |
Lentiviral plasmid list, maps and sequences information. [link]

Distribution information

Terms and conditions for distribution The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe Dr. Hiroyuki Miyoshi as the Developer.
Additional terms and conditions for distribution RECIPIENT is requested to obtain written consent of Dr. Atsushi Miyawaki of the RIKEN CBS regarding Venus fluorescent protein.
Ordering Please visit Ordering instruction. 
Order form [Credit Card Payment]  [Bank Transfer or Check Payment]
Material Transfer Agreement (MTA) [Word]
Approval form [Word] by Dr. Atsushi Miyawaki
Remarks ((Remarks)) Remember that you will be working with samples containing infectious virus.
Please contact us. We will provide you with appropriate documents.
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB04383 CSII-CMV-MCS-IRES2-Venus DNA solution

Ordering instruction of plasmids [in Japanese] [in English]

References and tips

Electronic file

Electronic file Sequence RDB04383z.seq
Electronic file Remarks, protocol and/or map (pdf) RDB04383.pdf

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB04383_A2Kmp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: IRES-F | Sequence: RDB04383_A2Kma.seq
>04383_04383_A2Km_IRES-R_E01_13.ab1
    1 GCTAGCGGCT TCGGCAGTAC GTTAGGGGGG GGGGAGGGAG AGGGGCGGAT CCGCGGCCGC
   61 TGCAGAATTC TCGAGACCGG TAGCGCTAGC GGATCTGACG GTTCACTAAA CCAGCTCTGC
  121 TTATATAGAC CTCCCACCGT ACACGCCTAC CGCCCATTTG CGTCAATGGG GCGGAGTTGT
  181 TACGACATTT TGGAAAGTCC CGTTGATTTT GGTGCCAAAA CAAACTCCCA TTGACGTCAA
  241 TGGGGTGGAG ACTTGGAAAT CCCCGTGAGT CAAACCGCTA TCCACGCCCA TTGATGTACT
  301 GCCAAAACCG CATCACCATG GTAATAGCGA TGACTAATAC GTAGATGTAC TGCCAAGTAG
  361 GAAAGTCCCA TAAGGTCATG TACTGGGCAT AATGCCAGGC GGGCCATTTA CCGTCATTGA
  421 CGTCAATAGG GGGCGTACTT GGCATATGAT ACACTTGATG TACTGCCAAG TGGGCAGTTT
  481 ACCGTAAATA CTCCACCCAT TGACGTCAAT GGAAAGTCCC TATTGGCGTT ACTATGGGAA
  541 CATACGTCAT TATTGACGTC AATGGGCGGG GGTCGTTGGG CGGTCAGCCA GGCGGGCCAT
  601 TTACCGTAAG TTATGTAACG CGGAACTCCA TATATGGGCT ATGAACTAAT GACCCCGTAA
  661 TTGATTACTA TTAATCGAAT TAATTCAAAG TGAATTTCTG CTGTCCCTGT AATAAACCCG
  721 AAAATTTTGA ATTTTTGTAA TTTGTTTTTG TAATTCTTTA GTTTGTATGT CTGTTGCTAT
  781 TATGTCTACT ATTCTTTCCC CTGCACTGTA CCCCCCAATC CCCCCTTTTC TTTTAAAATT
  841 GTGGATGAAT ACTGCCATTT GTAGATCCGT TCACTAATCG AATGGATCTG TCTCTGTCTC
  901 TCTCTCCACC TTCTTCTTCT ATTCCTTCGG GCCTGTCGGG TCCCCTCGGG GTTGGGAGGT
  961 GGGTCTGAAA CGATAATGGT GAATATCCCT GCCTAACTCT ATTCACTATA GAAAGTACAG
 1021 CAAAAAACTA TTCTTAAACC TACCCAAGCC TCCTACTATC ATTTATGAAT AATTTTTATA
 1081 TACCACAGCC CAATTTGTTA TGGTAAATCA ATCACAAACT TTGCCCAATT TATCTAAATT
1141 CCAATAATTC TTGGTTTCCA ATC
//
Primer: IRES-R | Sequence: RDB04383_A2Kmb.seq
>04383_04383_A2Km_IRES-F_D01_10.ab1
    1 GAGGCGTTAA TGTGTTTAGT CGAGGTTAAA AACGTCTAGG CCCCCCGAAC CACGGGGACG
   61 TGGTTTTCCT TTGAAAAACA CGATGATAAT ATGGCCACAA CCATGGTGAG CAAGGGCGAG
  121 GAGCTGTTCA CCGGGGTGGT GCCCATCCTG GTCGAGCTGG ACGGCGACGT AAACGGCCAC
  181 AAGTTCAGCG TGTCCGGCGA GGGCGAGGGC GATGCCACCT ACGGCAAGCT GACCCTGAAG
  241 CTGATCTGCA CCACCGGCAA GCTGCCCGTG CCCTGGCCCA CCCTCGTGAC CACCCTGGGC
  301 TACGGCCTGC AGTGCTTCGC CCGCTACCCC GACCACATGA AGCAGCACGA CTTCTTCAAG
  361 TCCGCCATGC CCGAAGGCTA CGTCCAGGAG CGCACCATCT TCTTCAAGGA CGACGGCAAC
  421 TACAAGACCC GCGCCGAGGT GAAGTTCGAG GGCGACACCC TGGTGAACCG CATCGAGCTG
  481 AAGGGCATCG ACTTCAAGGA GGACGGCAAC ATCCTGGGGC ACAAGCTGGA GTACAACTAC
  541 AACAGCCACA ACGTCTATAT CACCGCCGAC AAGCAGAAGA ACGGCATCAA GGCCAACTTC
  601 AAGATCCGCC ACAACATCGA GGACGGCGGC GTGCAGCTCG CCGACCACTA CCAGCAGAAC
  661 ACCCCCATCG GCGACGGCCC CGTGCTGCTG CCCGACAACC ACTACCTGAG CTACCAGTCC
  721 GCCCTGAGCA AAGACCCCAA CGAGAAGCGC GATCACATGG TCCTGCTGGA GTTCGTGACC
  781 GCCGCCGGGA TCACTCTCGG CATGGACGAG CTGTACAAGT AAGAATTCAA GGCCTCTCGA
  841 GCCTCTAGAG TTAACATCGA GGGATCAAGC TTATCGATAA TCAACCTCTG GATTACAAAA
  901 TTTGTGAAAG ATTGACTGGT ATTCTTAACT ATGTTGCTCC TTTTACGCTA TGTGGATACG
  961 CTGCTTTAAT GCCTTTGTAT CATGCTATTG CTTCCCGTAT GGCTTTCATT TTCTCCTCCC
 1021 TTGTATAAAT CCTGGTTTGC TGTCTCTTTT ATGAGGAGTT TGTGGGCCGT TTGTCAGCAA
 1081 CGTGGCGTGT GTGCAACTGT GATTTGCCTG AACGCACCCC AACTGCTTTG GGGGCATTGC
1141 CCACCCACCA GGGTTCCAGC CTTCCC
//
Primer: HIVgp_R1 | Sequence: RDB04383_A2Kmc.seq
>04383_04383_A2Km_HIVgp_R1_F01_16.ab1
    1 CCAGCTCTAT TCCACTGCTC TTTTTTCTCT CTGCACCACT CTTCTCTTTG CCTTGGTGGG
   61 TGCTACTCCT AATGGTTCAA TTTTTACTAC TTTATATTTA TATAATTCAC TTCTCCAATT
  121 GTCCCTCATA TCTCCTCCTC CAGGTCTGAA GATCAGCGGC CGGCCGCTTG CTGTGCGGTG
  181 GTCTTACTTT TGTTTTGCTC TTCCTCTATC TTGTCTAAAG CTTCCTTGGT GTCTTTTATC
  241 TCTATCCTTT GATGCACACA ATAGAGGGTT GCTACTGTAT TATATAATGA TCTAAGTTCT
  301 TCTGATCCTG TCTGAAGGGA TGGTTGTAGC TGTCCCAGTA TTTGTCTACA GCCTTCTGAT
  361 GTTTCTAACA GGCCAGGATT AACTGCGAAT CGTTCTAGCT CCCTGCTTGC CCATACTATA
  421 TGTTTTAATT TATATTTTTT CTTTCCCCCT GGCCTTAACC GAATTTTTTC CCATCGCGAT
  481 CTAATTCTCC CCCGCTTAAT ACTGACGCTC TCGCACCCAT CTCTCTCCTT CTAGCCTCCG
  541 CTAGTCAAAA TTTTTGGCGT ACTCACCAGT CGCCGCCCCT CGCCTCTTGC CGTGCGCGCT
  601 TCAGCAAGCC GAGTCCTGCG TCGAGAGAGC TCCTCTGGTT TCCCTTTCGC TTTCAAGTCC
  661 CTGTTCGGGC GCCACTGCTA GAGATTTTCC ACACTGACTA AAAGGGTCTG AGGGATCTCT
  721 AGTTACCAGA GTCACACAAC AGACGGGCAC ACACTACTTG AAGCACTCAA GGCAAGCTTT
  781 ATTGAGGCTT AAGCAGTGGG TTCCCTAGTT AGCCAGAGAG CTCCCAGGCT CAGATCTGGT
  841 CTAACCAGAG AGACCCAGTA CAGGCAAAAC GCGCTGCTTA TATAGACCTC CCACCGTACA
  901 CGCCTACCGC CCATTTGCGT CAATGGGGGC GGAGTTGTTA CGACATTTTG GAAAGTCCCG
  961 TTGATTTTGG TGCCAAAACA AACTCCCATT GACGTCAATG GGGTGGAGAC TTGGAAAATC
 1021 CCCGTGAGTC AAACCGCTAT CCACGCCCAT TGATGTACTG CCCAAACGCA TCACCATGGT
 1081 AATAGCGATG ACTAATACGT AGATGTACTG CCAAGTAGGA AAGTTCCATT CGTTCAATGT
1141 ACTGGGCATA TGCAGGCGGG TCCAATTTTA CCCGGTCCAT TGGAAGCG
//
Primer: BGH_rev | Sequence: RDB04383_A2Kmd.seq
>04383_04383_A2Km_BGHrev_G01_19.ab1
    1 GTTTCGGACT GCTAGAGATT TTCCACACTG ACTAAAAGGG TCTGAGGGAT CTCTAGTTAC
   61 CAGAGTCACA CAACAGACGG GCACACACTA CTTGAAGCAC TCAAGGCAAG CTTTATTGAG
  121 GCTTAAGCAG TGGGTTCCCT AGTTAGCCAG AGAGCTCCCA GGCTCAGATC TGGTCTAACC
  181 AGAGAGACCC AGTACAGTCC GGATGCAGCT CTCGGGCCAT GTGATGAAAT GCTAGGCGGC
  241 TGTCAAACCT CCACTCTAAT ACTTCTCTCT CCGGGTCATC CATCCCATGC AGGCTCACAG
  301 GGTGTAACAA GCGGGTGTTC TCTCCTTCAT TGGCTTCTTC TACCTTCTCT TGCTCAACTG
  361 GTACTAGCTT GTAGCACCAT CCAAAGGTCA GTGGATATCT GATCCCTGGC CCTGGTGTGT
  421 AGTTCTGCCA ATCAGGGAAG TAGCCTTGTG TGTGGTAGAT CCACAGATCA AGGATATCTT
  481 GTCTTCGTTG GGAGTGAATT AGCCCTTCCA GTCCCCCCTT TTCTTTTAAA AAGTGGCTAA
  541 GATCTACAGC TGCCTTGTAA GTCATTGGTC TTAAAGGTAC CTGAGGTGTG ACTGGAAAAC
  601 CCACCTCCTC CTCCTCTTGT GCTTCTAGCC AGGCACAATC AGCATTGGTA GCTGCTGTAT
  661 TGCTACTTGT GATTGCTCCA TGTTTTTCTA GGTCTCGACG GTATCGATGC GGGGAGGCGG
  721 CCCAAAGGGA GATCCGACTC GTCTGAGGGC GAAGGCGAAG ACGCGGAAGA GGCCGCAGAG
  781 CCGGCAGCAG GCCGCGGGAA GGAAGGTCCG CTGGATTGAG GGCCGAAGGG ACGTAGCAGA
  841 AGGACGTCCC GCGCAGAATC CAGGTGGCAA CACAGGCGAG CAGCCATGGA AAGGACGTCA
  901 GCTTCCCCGA CAACACCACG GAATTGTCAG TGCCCAACAG CCGAGCCCCT GTCCAGCAGC
  961 GGGCAAAGGC AGGCGGCGAT GAGTTCCGCC GTGGCAATAC GGGAGGGGGA AAGCGAAAGT
 1021 CCCGGGAAGG AGCTGACAGG TGGTGGCAAT GCCCCAACCC AGTGGGGGGT TGCGTCAGCA
 1081 AACACAGTGC ACACCACGCA CGTTGCCTGA CACGGGCCAC ACTTCTCATA AGAGACAGGC
1141 CATCAGATTT TATACAAGGA AGGGAAGAAA ATTGAAAAGC CCAATATACC CG
//
Primer: SV40pro_F | Sequence: RDB04383_A2Kme.seq
>04383_04383_A2Km_SV40pro_F_H01_22.ab1
    1 CCCTGTCCCT GGCATTCAGA GTAGTGAGGA GGCTTTTTTG GAGGCCTAGG CTTTTGCAAA
   61 AAGCTCCCGG GAGCTTGTAT ATCCATTTTC GGATCTGATC AGCACGTGTT GACAATTAAT
  121 CATCGGCATA GTATATCGGC ATAGTATAAT ACGACAAGGT GAGGAACTAA ACCATGGCCA
  181 AGTTGACCAG TGCCGTTCCG GTGCTCACCG CGCGCGACGT CGCCGGAGCG GTCGAGTTCT
  241 GGACCGACCG GCTCGGGTTC TCCCGGGACT TCGTGGAGGA CGACTTCGCC GGTGTGGTCC
  301 GGGACGACGT GACCCTGTTC ATCAGCGCGG TCCAGGACCA GGTGGTGCCG GACAACACCC
  361 TGGCCTGGGT GTGGGTGCGC GGCCTGGACG AGCTGTACGC CGAGTGGTCG GAGGTCGTGT
  421 CCACGAACTT CCGGGACGCC TCCGGGCCGG CCATGACCGA GATCGGCGAG CAGCCGTGGG
  481 GGCGGGAGTT CGCCCTGCGC GACCCGGCCG GCAACTGCGT GCACTTCGTG GCCGAGGAGC
  541 AGGACTGACA CGTGCTACGA GATTTCGATT CCACCGCCGC CTTCTATGAA AGGTTGGGCT
  601 TCGGAATCGT TTTCCGGGAC GCCGGCTGGA TGATCCTCCA GCGCGGGGAT CTCATGCTGG
  661 AGTTCTTCGC CCACCCCAAC TTGTTTATTG CAGCTTATAA TGGTTACAAA TAAAGCAATA
  721 GCATCACAAA TTTCACAAAT AAAGCATTTT TTTCACTGCA TTCTAGTTGT GGTTTGTCCA
  781 AACTCATCAA TGTATCTTAT CATGTCTGTA TACCGTCGAC CTCTAGCTAG AGCTTGGCGT
  841 AATCATGGTC ATAGCTGTTT CCTGTGTGAA ATTGTTATCC GCTCACAATT CCACACAACA
  901 TACGAGCCGG AAGCATAAAG TGTAAAGCCT GGGGTGCCTA AATGAGTGAG CTAACTCACA
  961 TTAATTGCGT TGCGCTCACT GCCCGCTTTC CAGTCGGGAA ACCTGTCGTG CCAGCTGCAT
 1021 TAATGAATCG GCCCAACGCG CGGGGAGAGG CGGTTTTGCG TATTGGCGCT CTTCCGCTTT
 1081 CTCGCTCACT GACTCGCTGC GCTCGCTCGC TCCGGCTGCC GGCGAGCCGG TAATCAGCTC
1141 ACTTCAAAGG CCGGTAAAAT TACCGGCTAA C
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

reference Ikemoto, M., Autologous transplantation of SM/C-2.6(+) satellite cells transduced with micro-dystrophin CS1 cDNA by lentiviral vector into mdx mice. Mo.l Ther. 15 (12): 2178-2185 (2007). PMID 17726457.
user report Ishibashi, D., Type I interferon protects neurons from prions in in vivo models. Brain 142 (4): 1035-1050 (2019). PMID 30753318.
user report Hui, K.K., GABARAPs dysfunction by autophagy deficiency in adolescent brain impairs GABAA receptor trafficking and social behavior. Sci. Adv. 5 (4): eaau8237 (2019). PMID 30989111.
user report Kikuchi, J., Soluble SLAMF7 promotes the growth of myeloma cells via homophilic interaction with surface SLAMF7. Leukemia. 2019 Jul 29. . [Epub ahead of print] PMID 31358854.
user report Watanabe, N., A pull-down and slot blot-based screening system for inhibitor compounds of the podoplanin-CLEC-2 interaction. PLoS One 14 (9): e0222331 (2019). PMID 31553741.
user report Kikuchi, J., Myeloma Cells are Activated in Bone Marrow Microenvironment by the CD180/MD-1 Complex which Senses Lipopolysaccharide. Cancer Res. 78 (7): 1766-1778 (2018). PMID 29363546.
user report Osada, N., Lysine-specific demethylase 1 inhibitors prevent teratoma development from human induced pluripotent stem cells. Oncotarget 9 (5): 6450-6462 (2018). PMID 29464084.
user report Uenaka, T., In silico drug screening by using genome-wide association study data repurposed dabrafenib, an anti-melanoma drug, for Parkinson's disease. Hum. Mol. Genet. 27 (22): 3974-3985 (2018). PMID 30137437.
user report Tanaka, M., Aggregation of scaffolding protein DISC1 dysregulates phosphodiesterase 4 in Huntington's disease. J. Clin. Invest. 127 (4): 1438-1450 (2017). PMID 28263187.
user report Su, Z., Neurocan, an extracellular chondroitin sulfate proteoglycan, stimulates neuroblastoma cells to promote malignant phenotypes. Oncotarget 8 (63): 106296-106310 (2017). PMID 29290949.
user report Sasamoto, Y., PAX6 Isoforms, along with Reprogramming Factors, Differentially Regulate the Induction of Cornea-specific Genes. Sci. Rep. 6: 20807, 2016. PMID 26899008.
user report Lu, F., Downregulation of CREB Promotes Cell Proliferation by Mediating G1/S Phase Transition in Hodgkin Lymphoma. Oncol. Res. 24 (3): 171-179 (2016). PMID 27458098.
user report Hikita, A., Analyses of bone modeling and remodeling using in vitro reconstitution system with two-photon microscopy. Bone 76: 5-17 (2015). PMID 25771421.
user report Minamino, M., Esco1 Acetylates Cohesin via a Mechanism Different from That of Esco2. Curr. Biol. 25 (13): 1694-1706 (2015). PMID 26051894.
user report Kikuchi, J., The novel orally active proteasome inhibitor K-7174 exerts anti-myeloma activity in vitro and in vivo by down-regulating the expression of class I histone deacetylases. J. Biol. Chem., 288 (35): 25593-25602 (2013). PMID 23878197.
user report Matsushita, M., Meclozine Facilitates Proliferation and Differentiation of Chondrocytes by Attenuating Abnormally Activated FGFR3 Signaling in Achondroplasia. PLoS One, 8 (12): e81569 (2013). PMID 24324705.
user report Kikuchi J., Histone deacetylases are critical targets of bortezomib-induced cytotoxicity in multiple myeloma. Blood., 116, 406-417 (2010). PMID 20351311.

2019.11.20

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