RDB04383 - CSII-CMV-MCS-IRES2-Venus

Resource data sheet
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CSII-CMV-MCS-IRES2-Venus

Backbone vector plasmid of lentivirus construct driven by CMV promoter with Venus fluorescent protein marker.

Catalog number	RDB04383
Resource name	CSII-CMV-MCS-IRES2-Venus
Clone info.	Backbone vector plasmid of lentivirus construct driven by CMV promoter with Venus fluorescent protein marker. Commonly requested with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Vector backbone	CSII-CMV-MCS (Plasmid)
Selectable markers	Please note that Zeo resistance marker is not included in the lentivirus produced from this vector. E. coli: Amp^r.
Gene/insert name	Aequorea victoria GFP cDNA
Depositor\|Developer	Miyoshi, Hiroyuki \|

Lentiviral plasmid list, maps and sequences information. [link]

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer.

Additional terms and conditions for distribution RECIPIENT is requested to obtain written consent of Dr. Atsushi Miyawaki of the RIKEN CBS regarding Venus fluorescent protein.

Ordering Please visit Ordering instruction.[link]
Order form [Credit Card Payment] [Bank Transfer or Check Payment]
Material Transfer Agreement (MTA, for use for not-for-profit academic purpose)[Word]
Approval form [Word] by Dr. Atsushi Miyawaki

Remarks ((Remarks)) Remember that you will be working with samples containing infectious virus.
Please contact us. We will provide you with appropriate documents.
Please obtain written approval of Dr. Atsushi Miyawaki.

Distribution information (domestic users) [open/close]

Terms and conditions set forth by the DEPOSITOR	The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer.
Additional terms and conditions for distribution	RECIPIENT is requested to obtain written consent of Dr. Atsushi Miyawaki of the RIKEN CBS regarding Venus fluorescent protein.
Ordering	Please visit Ordering instruction.[link] Order form [Credit Card Payment] [Bank Transfer or Check Payment] Material Transfer Agreement (MTA, for use for not-for-profit academic purpose)[Word] Approval form [Word] by Dr. Atsushi Miyawaki
Remarks	((Remarks)) Remember that you will be working with samples containing infectious virus. Please contact us. We will provide you with appropriate documents. Please obtain written approval of Dr. Atsushi Miyawaki.

提供条件	本件研究材料は、非営利機関の非営利学術研究に限って提供する。本件研究材料を利用した研究結果等を発表する際は、本件研究材料が故三好浩之博士により開発されたことを明示する。
付加的提供条件	利用者は、Venus蛍光タンパク質について理化学研究所CBS 宮脇敦史博士から、書面による提供承諾を得ること。
提供依頼	手続きの詳細は、「レンチウイルスベクターの提供申し込み」をご覧ください。提供依頼書 [Word] 提供同意書 (非営利機関による非営利学術研究用)[Word] 遺伝子組換え生物の受入れ確認書 [Word] 宮脇敦史博士による提供承諾書 [Word]
備考	【使用上の注意】このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。宮脇敦史博士の書面による提供承諾が必要です。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB04383	CSII-CMV-MCS-IRES2-Venus	DNA solution

Ordering instruction of plasmids [in Japanese] [in English]
Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

References and tips

Electronic file

Electronic file	Sequence RDB04383z.seq
Electronic file	Remarks, protocol and/or map (pdf) RDB04383.pdf

Original reference

Further references such as user reports and related articles (go to bottom)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB17904_B0Aep1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: IRES-R Region: CMV pro2, CMV en2, cPPT_CTS Sequence file: RDB17904_B0Aea.seq
>04383_17904_B0Ae_1_IRES-R_A08_02_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN TTANGGGGGG GGGNNGGNNN NGGGCGGNNC CGCGGCCGCT 61 GCAGAATTCT CGAGACCGGT AGCGCTAGCG GATCTGACGG TTCACTAAAC CAGCTCTGCT 121 TATATAGACC TCCCACCGTA CACGCCTACC GCCCATTTGC GTCAATGGGG CGGAGTTGTT 181 ACGACATTTT GGAAAGTCCC GTTGATTTTG GTGCCAAAAC AAACTCCCAT TGACGTCAAT 241 GGGGTGGAGA CTTGGAAATC CCCGTGAGTC AAACCGCTAT CCACGCCCAT TGATGTACTG 301 CCAAAACCGC ATCACCATGG TAATAGCGAT GACTAATACG TAGATGTACT GCCAAGTAGG 361 AAAGTCCCAT AAGGTCATGT ACTGGGCATA ATGCCAGGCG GGCCATTTAC CGTCATTGAC 421 GTCAATAGGG GGCGTACTTG GCATATGATA CACTTGATGT ACTGCCAAGT GGGCAGTTTA 481 CCGTAAATAC TCCACCCATT GACGTCAATG GAAAGTCCCT ATTGGCGTTA CTATGGGAAC 541 ATACGTCATT ATTGACGTCA ATGGGCGGGG GTCGTTGGGC GGTCAGCCAG GCGGGCCATT 601 TACCGTAAGT TATGTAACGC GGAACTCCAT ATATGGGCTA TGAACTAATG ACCCCGTAAT 661 TGATTACTAT TAATCGAATT AATTCAAAGT GAATTTCTGC TGTCCCTGTA ATAAACCCGA 721 AAATTTTGAA TTTTTGTAAT TTGTTTTTGT AATTCTTTAG TTTGTATGTC TGTTGCTATT 781 ATGTCTACTA TTCTTTCCCC TGCACTGTAC CCCCCAATCC CCCCCTTTTC TTTTAAAATT 841 GTGGATGAAT ACTGCCATTT GTAGATCCNN NCACTAATCG AATGGATCTG TCTCTGTCTC 901 TNTCTNCACC TTCTTCTTCN ATTCCNTCNG NNNNNNCNGG NNCNNNNNNG NNNNTNNGNN 961 NNNNGNNNNN NNNNN //
Primer: IRES-F Region: IRES2, Venus Sequence file: RDB17904_B0Aeb.seq
>04383_17904_B0Ae_1_IRES-F_B12_06_ABI24.ab1 1 NNNNNNNNNN NNNNNNTNNG TCNAGGTTNN NNNNNGTCTA GGCCCCCCGA ACCNCGGGGA 61 CGTGGTTTTC CTTTGAAAAA CACGATGATA ATATGGCCAC AACCATGGTG AGCAAGGGCG 121 AGGAGCTGTT CACCGGGGTG GTGCCCATCC TGGTCGAGCT GGACGGCGAC GTAAACGGCC 181 ACAAGTTCAG CGTGTCCGGC GAGGGCGAGG GCGATGCCAC CTACGGCAAG CTGACCCTGA 241 AGCTGATCTG CACCACCGGC AAGCTGCCCG TGCCCTGGCC CACCCTCGTG ACCACCCTGG 301 GCTACGGCCT GCAGTGCTTC GCCCGCTACC CCGACCACAT GAAGCAGCAC GACTTCTTCA 361 AGTCCGCCAT GCCCGAAGGC TACGTCCAGG AGCGCACCAT CTTCTTCAAG GACGACGGCA 421 ACTACAAGAC CCGCGCCGAG GTGAAGTTCG AGGGCGACAC CCTGGTGAAC CGCATCGAGC 481 TGAAGGGCAT CGACTTCAAG GAGGACGGCA ACATCCTGGG GCACAAGCTG GAGTACAACT 541 ACAACAGCCA CAACGTCTAT ATCACCGCCG ACAAGCAGAA GAACGGCATC AAGGCCAACT 601 TCAAGATCCG CCACAACATC GAGGACGGCG GCGTGCAGCT CGCCGACCAC TACCAGCAGA 661 ACACCCCCAT CGGCGACGGC CCCGTGCTGC TGCCCGACAA CCACTACCTG AGCTACCAGT 721 CCGCCCTGAG CAAAGACCCC AACGAGAAGC GCGATCACAT GGTCCTGCTG GAGTTCGTGA 781 CCGCCGCCGG GNTCACTCTC GGCATGGACG ANCTGTACAA GTAAGANTTC AAGGCCTCTC 841 GAGCCTCTAG AGTTAACATC GAGGGATCAA GCTTATCGAT AATCAACCTC TGGATTACAA 901 AATTTGTGAA AGANTGANTG GNATTCTTAA CTATGTTGNT NCTTTNACNN TATGNGGATA 961 CNCNGCTTTN ATGCCTTTGT ATCATGCTAT NGNTTNCNNN ATGGCTTTCA TTTNNNNNNN 1021 CNNNNNNNNA NNNCNNNNNN NNNN //
Primer: PRE_rev Region: PRE, Venus Sequence file: RDB17904_B0Aec.seq
>04383_17904_B0Ae_1_PRE_rev_B08_05_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNG NNCNNNNTAG TTAAGAATAC CAGTCAATCT TTCNCNAATT 61 TTGTAATCCA GAGGTTGATT ATCGATAAGC TTGATCCCTC GATGTTAACT CTAGAGGCTC 121 GAGAGGCCTT GAATTCTTAC TTGTACAGCT CGTCCATGCC GAGAGTGATC CCGGCGGCGG 181 TCACGAACTC CAGCAGGACC ATGTGATCGC GCTTCTCGTT GGGGTCTTTG CTCAGGGCGG 241 ACTGGTAGCT CAGGTAGTGG TTGTCGGGCA GCAGCACGGG GCCGTCGCCG ATGGGGGTGT 301 TCTGCTGGTA GTGGTCGGCG AGCTGCACGC CGCCGTCCTC GATGTTGTGG CGGATCTTGA 361 AGTTGGCCTT GATGCCGTTC TTCTGCTTGT CGGCGGTGAT ATAGACGTTG TGGCTGTTGT 421 AGTTGTACTC CAGCTTGTGC CCCAGGATGT TGCCGTCCTC CTTGAAGTCG ATGCCCTTCA 481 GCTCGATGCG GTTCACCAGG GTGTCGCCCT CGAACTTCAC CTCGGCGCGG GTCTTGTAGT 541 TGCCGTCGTC CTTGAAGAAG ATGGTGCGCT CCTGGACGTA GCCTTCGGGC ATGGCGGACT 601 TGAAGAAGTC GTGCTGCTTC ATGTGGTCGG GGTAGCGGGC GAAGCACTGC AGGCCGTANC 661 CCAGGGTGGT CACGANGGTG GGCCAGGGCA CGGGCAGCTT GCCGGTGGTG CAGATCAGCT 721 TCAGGGTCAG CTTGCCGTAN GTGGCATCGC CCTCGCCCTC GCCGGACACG CTGAACTTGT 781 GGCCGTTTAC GTCGCCGTCC AGCTCGACCA GGANGGGCAC CACCNCGGNG AACAGCTCCT 841 CGCCCTNNCT CACCATGGTT GNGGNCATAT TATCATCGNG TTTTTTCAAA NGAAAANNNN 901 NNNCNNNNNN NNCGGGGGGN NNNANNNNNT TTTTTTNNNN CNNNNANNAA NNNNNNNNNA 961 NNNNNNNNNN //
Primer: HIV-B_R Region: HIV-1 psi, 5' LTR, CMV pro1, CMV en1 Sequence file: RDB17904_B0Aed.seq
>04383_17904_B0Ae_1_HIV-B_R_C08_08_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNTTTGGCG TANTCNCCAG TCGCCGCCCC TCGCCTCTTG 61 CCGTGCGCGC TTCAGCAAGC CGAGTCCTGC GTCGAGAGAG CTCCTCTGGT TTCCCTTTCG 121 CTTTCAAGTC CCTGTTCGGG CGCCACTGCT AGAGATTTTC CACACTGACT AAAAGGGTCT 181 GAGGGATCTC TAGTTACCAG AGTCACACAA CAGACGGGCA CACACTACTT GAAGCACTCA 241 AGGCAAGCTT TATTGAGGCT TAAGCAGTGG GTTCCCTAGT TAGCCAGAGA GCTCCCAGGC 301 TCAGATCTGG TCTAACCAGA GAGACCCAGT ACAGGCAAAA CGCGCTGCTT ATATAGACCT 361 CCCACCGTAC ACGCCTACCG CCCATTTGCG TCAATGGGGC GGAGTTGTTA CGACATTTTG 421 GAAAGTCCCG TTGATTTTGG TGCCAAAACA AACTCCCATT GACGTCAATG GGGTGGAGAC 481 TTGGAAATCC CCGTGAGTCA AACCGCTATC CACGCCCATT GATGTACTGC CAAAACCGCA 541 TCACCATGGT AATAGCGATG ACTAATACGT AGATGTACTG CCAAGTAGGA AAGTCCCATA 601 AGGTCATGTA CTGGGCATAA TGCCAGGCGG GCCATTTACC GTCATTGACG TCAATAGGGG 661 GCGTACTTGG CATATGATAC ACTTGATGTA CTGCCAAGTG GGCAGTTTAC CGTAAATACT 721 CCACCCATTG ACGTCAATGG AAAGTCCCTA TTGGCGTTAC TATGGGAACA TACGTCATTA 781 TTGACGTCAA TGGGCGGGGG TCGTTGGGCG GTCAGCCAGG CGGGCCATTT ANCNNAAGTT 841 ATGTAACGCG GAANTNNNTA TATGGGCTAT NAANTNNTGA NNNNNNAATT GANTACNANT 901 AATAACNNNN CNANAATCNN NNNCNNNNNN NANATCNGNN CNNNNNNTNN NNNANNNNNN 961 NNNN //
Primer: RRE-M_R Region: RRE, HIV-1 psi Sequence file: RDB17904_B0Aee.seq
>04383_17904_B0Ae_1_RRE_M_R_D08_11_ABI24.ab1 1 NNNNNNNNNN NNNNNNNGCT GTTGCGCCTC NNTAGCCCTC NGCAAATTGT TCTGCTGCTG 61 CACTATACCA GACAATAATT GTCTGGCCTG TACCGTCAGC GTCATTGACG CTGCGCCCAT 121 AGTGCTTCCT GCTGCTCCCA AGAACCCAAG GAACAAAGCT CCTATTCCCA CTGCTCTTTT 181 TTCTCTCTGC ACCACTCTTC TCTTTGCCTT GGTGGGTGCT ACTCCTAATG GTTCAATTTT 241 TACTACTTTA TATTTATATA ATTCACTTCT CCAATTGTCC CTCATATCTC CTCCTCCAGG 301 TCTGAAGATC AGCGGCCGGC CGCTTGCTGT GCGGTGGTCT TACTTTTGTT TTGCTCTTCC 361 TCTATCTTGT CTAAAGCTTC CTTGGTGTCT TTTATCTCTA TCCTTTGATG CACACAATAG 421 AGGGTTGCTA CTGTATTATA TAATGATCTA AGTTCTTCTG ATCCTGTCTG AAGGGATGGT 481 TGTAGCTGTC CCAGTATTTG TCTACAGCCT TCTGATGTTT CTAACAGGCC AGGATTAACT 541 GCGAATCGTT CTAGCTCCCT GCTTGCCCAT ACTATATGTT TTAATTTATA TTTTTTCTTT 601 CCCCCTGGCC TTAACCGAAT TTTTTCCCAT CGCGATCTAA TTCTCCCCCG CTTAATACTG 661 ACGCTCTCGC ACCCATCTCT CTCCTTCTAG CCTCCGCTAG TCAAAATTTT TGGCGTACTC 721 ACCAGTCGCC GCCCCTCGCC TCTTGCCGTG CGCGCTTCAG CAAGCCGANT CCTGCGTCGA 781 GAGAGCTCCT CTGGTTTCCC TTTCGCTTTC AAGTCCCTGT TCGGGCGCCA CTGCTAGANA 841 NTTTNCACAC TGACNNAAAN GGTCTGANGG NNCTCTANTT ANCANANNCA CACNACANAN 901 NGGCNCACAC NACTNNAANC ANTNNNNNNN AGCTTTANNG ANGNNNNANC NNNNGGNTNN 961 CNNNNNTNNN NNNNNNN //
Primer: cPPT_CTS-E_R Region: cPPT_CTS, RRE Sequence file: RDB17904_B0Aef.seq
>04383_17904_B0Ae_1_cPPT_CTS_E_R_E08_14_ABI24.ab1 1 NNNNNNNNNN NNNNNTNGNA TTCTTNAGTT TGTATGTCTG TTGCTATTAT GTCTACTATT 61 CTTTCCCCTG CACTGTACCC CCCAATCCCC CCTTTTCTTT TAAAATTGTG GATGAATACT 121 GCCATTTGTA GATCCGTTCA CTAATCGAAT GGATCTGTCT CTGTCTCTCT CTCCACCTTC 181 TTCTTCTATT CCTTCGGGCC TGTCGGGTCC CCTCGGGGTT GGGAGGTGGG TCTGAAACGA 241 TAATGGTGAA TATCCCTGCC TAACTCTATT CACTATAGAA AGTACAGCAA AAACTATTCT 301 TAAACCTACC AAGCCTCCTA CTATCATTAT GAATAATTTT ATATACCACA GCCAATTTGT 361 TATGTTAAAC CAATTCCACA AACTTGCCCA TTTATCTAAT TCCAATAATT CTTGTTCATT 421 CTTTTCTTGC TGGTTTTGCG ATTCTTCAAT TAAGGAGTGT ATTAAGCTTG TGTAATTGTT 481 AATTTCTCTG TCCCACTCCA TCCAGGTCGT GTGATTCCAA ATCTGTTCCA GAGATTTATT 541 ACTCCAACTA GCATTCCAAG GCACAGCAGT GGTGCAAATG AGTTTTCCAG AGCAACCCCA 601 AATCCCCAGG NGCTGTTGAT CCTTTAGGTA TCTTTCCACA GCCAGGATTC TTGCCTGGAG 661 CTGCTTGATG CCCCAGACTG TGAGTTGCAA CAGATGCTGT TGCGCCTCAA TAGCCCTCAG 721 CAAATTGTTC TGCTGCTGCA CTATACCAGA CAATAATTGT CTGGCCTGTA CCGTCAGCGT 781 CATTGACGCT GCGCCCATAG TGCTTCCTGC TGCTCCCAAG AACCCAAGGA ACAAAGCTCC 841 TATTCCCACN GCTCTTTTTT TCTCTCTGCA CCACTCTTCN CTTNGCCNNN GNGGGNGCNA 901 NNNCNNNNGG NTCAANTTTT TACTNNNTTN NATTTANNNN NTTCNCTNNN NCNANTNNNN 961 CNNNNNNNNN N //
Primer: BGH_rev2 Region: bGH p(A), 3' LTR, Sequence file: RDB17904_B0Aeg.seq
>04383_17904_B0Ae_1_BGH_rev2_F08_17_ABI24.ab1 1 NNNNNNNNNN NNNNNGGGGG NNGGGCNNNC NACAGATGGC TGGCAACTAG AAGGCACAGT 61 CGAGGCTGAT CAGCGGGTTT AAACGGGCCC TGCTAGAGAT TTTCNACACT GACTAAAAGG 121 GTCTGAGGGA TCTCTAGTTA CCAGAGTCAC ACAACAGACG GGCACACACT ACTTGAAGCA 181 CTCAAGGCAA GCTTTATTGA GGCTTAAGCA GTGGGTTCCC TAGTTAGCCA GAGAGCTCCC 241 AGGCTCAGAT CTGGTCTAAC CAGAGAGACC CAGTACAGTC CGGATGCAGC TCTCGGGCCA 301 TGTGATGAAA TGCTAGGCGG CTGTCAAACC TCCACTCTAA TACTTCTCTC TCCGGGTCAT 361 CCATCCCATG CAGGCTCACA GGGTGTAACA AGCGGGTGTT CTCTCCTTCA TTGGCTTCTT 421 CTACCTTCTC TTGCTCAACT GGTACTAGCT TGTAGCACCA TCCAAAGGTC AGTGGATATC 481 TGATCCCTGG CCCTGGTGTG TAGTTCTGCC AATCNNNNNN GTAGCCTTGT GTGTGGTAGA 541 TCCACAGATC AAGGATATCT TGTCTTCGTT GGGAGTGAAT TAGCCCTTCC AGTCCCCCCT 601 TTTCTTTTAA AAAGTGGCTA AGATCTACAG CTGCCTTGTA AGTCATTGGT CTTAAAGGTA 661 CCTGANGTGT GACTGGAAAA CCCACCTCCT CCTCCTCTTG TGCTTCTAGC CAGGCACAAT 721 CAGCATTGGT AGCTGCTGTA TTGCTACTTG TGATTGCTCC ATGTTTTTCT AGGTCTCGNC 781 GGTATCGATG CGGGGANGCG GCCCNNAGGG ANNTNNGACT CGTCTGANGG CGAANGCGAA 841 NNNNNNNAAN ANGNCGCANA NNNNGCAGCN NNNCGNNNGA AGGNANNNNN CGNNNGNTTN 901 NNGNNCGNAN GGNANNNANN NNNNNNANNN NNNNCGCANN NNNNNNNN //
Primer: SV40pro_ori_F Region: SV40pro_ori, Zeo Sequence file: RDB17904_B0Aeh.seq
>04383_17904_B0Ae_1_SV40pro_ori_F_G08_20_ABI24.ab1 1 NNNNNNNNNN NNNNNCCTCT GCCTCTGNNC TATTCNNGAA GTAGTGAGGA GGCTTTTTTG 61 GAGGCCTAGG CTTTTGCAAA AAGCTCCCGG GAGCTTGTAT ATCCATTTTC GGATCTGATC 121 AGCACGTGTT GACAATTAAT CATCGGCATA GTATATCGGC ATAGTATAAT ACGACAAGGT 181 GAGGAACTAA ACCATGGCCA AGTTGACCAG TGCCGTTCCG GTGCTCACCG CGCGCGACGT 241 CGCCGGAGCG GTCGAGTTCT GGACCGACCG GCTCGGGTTC TCCCGGGACT TCGTGGAGGA 301 CGACTTCGCC GGTGTGGTCC GGGACGACGT GACCCTGTTC ATCAGCGCGG TCCAGGACCA 361 GGTGGTGCCG GACAACACCC TGGCCTGGGT GTGGGTGCGC GGCCTGGACG AGCTGTACGC 421 CGAGTGGTCG GAGGTCGTGT CCACGAACTT CCGGGACGCC TCCGGGCCGG CCATGACCGA 481 GATCGGCGAG CAGCCGTGGG GGCGGGAGTT CGCCCTGCGC GACCCGGCCG GCAACTGCGT 541 GCACTTCGTG GCCGAGGAGC AGGACTGACA CGTGCTACGA GATTTCGATT CCACCGCCGC 601 CTTCTATGAA AGGTTGGGCT TCGGAATCGT TTTCCGGGAC GCCGGCTGGA TGATCCTCNN 661 GCGCGGGGNT CTCATGCTGG AGTTCTTCGC CCACCCCAAC TTGTTTATTG CAGCTTATAA 721 TGGTTACNAA TAAAGCAATA GCATCACAAA TTTCACAAAT AAAGCATTTT TTTCNCTGNA 781 TTCTAGTTGT GGTTTGNNCN AACTCATCNA TGTATCTTAT CATGTCTGTA TANCNNCNAN 841 CTCTANCTAN NNCTNGGCGN AATCNTGGNC NTANCTGTTT CCNNNNNNAA ANTNNNATNN 901 NNNNNCNATN NNNCNCNNNN TNCGANNNNN NNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

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2020.12.24

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