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Backbone vector plasmid of lentivirus construct driven by CMV promoter with Venus fluorescent protein marker.

Catalog number RDB04383
Resource name CSII-CMV-MCS-IRES2-Venus
Clone info. Backbone vector plasmid of lentivirus construct driven by CMV promoter with Venus fluorescent protein marker.
Comment Commonly requested with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394).
Vector backbone CSII-CMV-MCS (Plasmid)
Selectable markers Ampicillin (E. coli). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Gene/insert name Aequorea victoria GFP cDNA
Depositor|Developer Miyoshi, Hiroyuki |
Sequence (full) RDB04383hts01.seqcheck assembled from experimentally sequenced data
Remarks, protocol and/or map (pdf) RDB04383.pdf
Test sheet RDB17904_B0Aup1-2.pdf 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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MTA, for use for not-for-profit academic purpose [Word]
Please visit Ordering instruction.[link] 
Terms and conditions for distribution The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer.
Remarks ((Remarks)) Remember that you will be working with samples containing infectious virus.
Please contact us. We will provide you with appropriate documents.
提供案内 (日本国内) [open/close]

提供依頼書 [Word]
提供同意書 (MTA, 非営利学術目的用)[Word]
提供条件 本件研究材料は、非営利機関の非営利学術研究に限って提供する。本件研究材料を利用した研究結果等を発表する際は、本件研究材料が故三好浩之博士により開発されたことを明示する。
備考 【使用上の注意】このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB04383 CSII-CMV-MCS-IRES2-Venus DNA solution

Ordering instruction of plasmids [in Japanese] [in English]

How to cite this biological resource

Materials & Methods section:

The CSII-CMV-MCS-IRES2-Venus was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB04383).

Reference section:

Further references such as user reports and related articles (go to bottom)


Original, user report and related articles

reference Ikemoto, M., Autologous transplantation of SM/C-2.6(+) satellite cells transduced with micro-dystrophin CS1 cDNA by lentiviral vector into mdx mice. Mo.l Ther. 15 (12): 2178-2185 (2007). PMID 17726457.
user_report Kikuchi, J., Soluble SLAMF7 promotes the growth of myeloma cells via homophilic interaction with surface SLAMF7. Leukemia 34 (1): 180-195 (2020). PMID 31358854. [link to RRC of NBRP]
user_report Ishibashi, D., Type I interferon protects neurons from prions in in vivo models. Brain 142 (4): 1035-1050 (2019). PMID 30753318. [link to RRC of NBRP]
user_report Hui, K.K., GABARAPs dysfunction by autophagy deficiency in adolescent brain impairs GABAA receptor trafficking and social behavior. Sci. Adv. 5 (4): eaau8237 (2019). PMID 30989111. [link to RRC of NBRP]
user_report Watanabe, N., A pull-down and slot blot-based screening system for inhibitor compounds of the podoplanin-CLEC-2 interaction. PLoS One 14 (9): e0222331 (2019). PMID 31553741. [link to RRC of NBRP]
user_report Kikuchi, J., Myeloma Cells are Activated in Bone Marrow Microenvironment by the CD180/MD-1 Complex which Senses Lipopolysaccharide. Cancer Res. 78 (7): 1766-1778 (2018). PMID 29363546. [link to RRC of NBRP]
user_report Osada, N., Lysine-specific demethylase 1 inhibitors prevent teratoma development from human induced pluripotent stem cells. Oncotarget 9 (5): 6450-6462 (2018). PMID 29464084. [link to RRC of NBRP]
user_report Uenaka, T., In silico drug screening by using genome-wide association study data repurposed dabrafenib, an anti-melanoma drug, for Parkinson's disease. Hum. Mol. Genet. 27 (22): 3974-3985 (2018). PMID 30137437. [link to RRC of NBRP]
user_report Tanaka, M., Aggregation of scaffolding protein DISC1 dysregulates phosphodiesterase 4 in Huntington's disease. J. Clin. Invest. 127 (4): 1438-1450 (2017). PMID 28263187. [link to RRC of NBRP]
user_report Su, Z., Neurocan, an extracellular chondroitin sulfate proteoglycan, stimulates neuroblastoma cells to promote malignant phenotypes. Oncotarget 8 (63): 106296-106310 (2017). PMID 29290949. [link to RRC of NBRP]
user_report Sasamoto, Y., PAX6 Isoforms, along with Reprogramming Factors, Differentially Regulate the Induction of Cornea-specific Genes. Sci. Rep. 6: 20807, 2016. PMID 26899008. [link to RRC of NBRP]
user_report Lu, F., Downregulation of CREB Promotes Cell Proliferation by Mediating G1/S Phase Transition in Hodgkin Lymphoma. Oncol. Res. 24 (3): 171-179 (2016). PMID 27458098. [link to RRC of NBRP]
user_report Hikita, A., Analyses of bone modeling and remodeling using in vitro reconstitution system with two-photon microscopy. Bone 76: 5-17 (2015). PMID 25771421. [link to RRC of NBRP]
user_report Minamino, M., Esco1 Acetylates Cohesin via a Mechanism Different from That of Esco2. Curr. Biol. 25 (13): 1694-1706 (2015). PMID 26051894. [link to RRC of NBRP]
user_report Kikuchi, J., The novel orally active proteasome inhibitor K-7174 exerts anti-myeloma activity in vitro and in vivo by down-regulating the expression of class I histone deacetylases. J. Biol. Chem., 288 (35): 25593-25602 (2013). PMID 23878197. [link to RRC of NBRP]
user_report Matsushita, M., Meclozine Facilitates Proliferation and Differentiation of Chondrocytes by Attenuating Abnormally Activated FGFR3 Signaling in Achondroplasia. PLoS One, 8 (12): e81569 (2013). PMID 24324705. [link to RRC of NBRP]
user_report Kikuchi J., Histone deacetylases are critical targets of bortezomib-induced cytotoxicity in multiple myeloma. Blood., 116, 406-417 (2010). PMID 20351311. [link to RRC of NBRP]