Please review the QC test results indicated by icon below as well as clone information before placing your order.

CS-CDF-EG-PRE

Lentivirus vector plasmid of GFP driven by human EF-1 alpha promoter.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer.
Ordering	Please visit Ordering instruction.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA, for use for not-for-profit academic purpose)[Word] This RESOURCE contains a fluorescent protein owned or licensed by Global Life Sciences Solutions Operations UK Ltd. (Cytiva). Please refer to Schedule B and use Schedule A (download the Schedule A and Schedule B [link]).
Remarks	((Remarks)) Remember that you will be working with samples containing infectious virus.

提供案内 (日本国内) [open/close]

提供条件	本件研究材料は、非営利機関の非営利学術研究に限って提供する。本件研究材料を利用した研究結果等を発表する際は、本件研究材料が故三好浩之博士により開発されたことを明示する。
提供依頼	手続きの詳細は、「レンチウイルスベクターの提供申し込み」をご覧ください。 提供依頼書 [Word] 提供同意書 (非営利機関による非営利学術研究用)[Word] GFPリソースを含みます。Schedule Bをご一読の上Schedule A (英語版のみ) の提出をお願いします （Schedule AとSchedule Bを入手[link])。 遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。
備考	【使用上の注意】このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB15736_A8Ahp1-3.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: EF1a-C_F Region: EF-1a pro,EGFP,WPRE Sequence file: RDB15736_A8Aha.seq
>04380_15736_A8Ah_EF1a-C_F_D01_04_ABI08.ab1     1 GCCTTTTTGG ATTTGCCCTT TTTGAGTTTG GATCTTGGTT CATTCTCAAG CCTCAGACAG    61 TGGTTCAAAG TTTTTTTCTT CCATTTCAGG TGTCGTGAAC ACGCTACCGG TCGCCACCAT   121 GGTGAGCAAG GGCGAGGAGC TGTTCACCGG GGTGGTGCCC ATCCTGGTCG AGCTGGACGG   181 CGACGTAAAC GGCCACAAGT TCAGCGTGTC CGGCGAGGGC GAGGGCGATG CCACCTACGG   241 CAAGCTGACC CTGAAGTTCA TCTGCACCAC CGGCAAGCTG CCCGTGCCCT GGCCCACCCT   301 CGTGACCACC CTGACCTACG GCGTGCAGTG CTTCAGCCGC TACCCCGACC ACATGAAGCA   361 GCACGACTTC TTCAAGTCCG CCATGCCCGA AGGCTACGTC CAGGAGCGCA CCATCTTCTT   421 CAAGGACGAC GGCAACTACA AGACCCGCGC CGAGGTGAAG TTCGAGGGCG ACACCCTGGT   481 GAACCGCATC GAGCTGAAGG GCATCGACTT CAAGGAGGAC GGCAACATCC TGGGGCACAA   541 GCTGGAGTAC AACTACAACA GCCACAACGT CTATATCATG GCCGACAAGC AGAAGAACGG   601 CATCAAGGTG AACTTCAAGA TCCGCCACAA CATCGAGGAC GGCAGCGTGC AGCTCGCCGA   661 CCACTACCAG CAGAACACCC CCATCGGCGA CGGCCCCGTG CTGCTGCCCG ACAACCACTA   721 CCTGAGCACC CAGTCCGCCC TGAGCAAAGA CCCCAACGAG AAGCGCGATC ACATGGTCCT   781 GCTGGAGTTC GTGACCGCCG CCGGGATCAC TCTCGGCATG GACGAGCTGT ACAAGTCCGG   841 ACTCAGATCC TACTAGTAGG ATCTCGAGGG ATCAAGCTTA TCGATAATCA ACCTCTGGAT   901 TACAAAATTT GTGAAAGATT GACTGGTATT CTTAACTATG TTGCTCCTTT TTACGCTATG   961 TGGATACGCT GCTTTAATGC CTTTTGTATC ATGCTATTGC TTCCCGTATG GCTTTCATTT  1021 TTCTCCTTCC TTTGTATAAA TTCCTATTTG CTGTCTCTTT ATGAGGAGTT GTGGCCCGTT  1081 GTCAGGCAAC GTGCGTGGTG TGCCACTGTG TTGCTGGACG CAACCCTCTC ACTTGGCTGG 1141 GGCATTGGCC AACACTGCTC AGCTTCTTTA CCGGAAACCA T //
Primer: CMV_Forward Region: CMVpro,5'LTR,HIV-1psi,RRE Sequence file: RDB15736_A8Ahb.seq
>04380_15736_A8Ah_CMV-Forward_C01_03_ABI08.ab1     1 CTGTTAAAAC TTCCGCCCCA TTGACGCAAA TGGGCGGTAG GCGTGTACGG TGGGAGGTCT    61 ATATAAGCAG CGCGTTTTGC CTGTACTGGG TCTCTCTGGT TAGACCAGAT CTGAGCCTGG   121 GAGCTCTCTG GCTAACTAGG GAACCCACTG CTTAAGCCTC AATAAAGCTT GCCTTGAGTG   181 CTTCAAGTAG TGTGTGCCCG TCTGTTGTGT GACTCTGGTA ACTAGAGATC CCTCAGACCC   241 TTTTAGTCAG TGTGGAAAAT CTCTAGCAGT GGCGCCCGAA CAGGGACTTG AAAGCGAAAG   301 GGAAACCAGA GGAGCTCTCT CGACGCAGGA CTCGGCTTGC TGAAGCGCGC ACGGCAAGAG   361 GCGAGGGGCG GCGACTGGTG AGTACGCCAA AAATTTTGAC TAGCGGAGGC TAGAAGGAGA   421 GAGATGGGTG CGAGAGCGTC AGTATTAAGC GGGGGAGAAT TAGATCGCGA TGGGAAAAAA   481 TTCGGTTAAG GCCAGGGGGA AAGAAAAAAT ATAAATTAAA ACATATAGTA TGGGCAAGCA   541 GGGAGCTAGA ACGATTCGCA GTTAATCCTG GCCTGTTAGA AACATCAGAA GGCTGTAGAC   601 AAATACTGGG ACAGCTACAA CCATCCCTTC AGACAGGATC AGAAGAACTT AGATCATTAT   661 ATAATACAGT AGCAACCCTC TATTGTGTGC ATCAAAGGAT AGAGATAAAA GACACCAAGG   721 AAGCTTTAGA CAAGATAGAG GAAGAGCAAA ACAAAAGTAA GACCACCGCA CAGCAAGCGG   781 CCGCTGATCT TCAGACCTGG AGGAGGAGAT ATGAGGGACA ATTGGAGAAG TGAATTATAT   841 AAATATAAAG TAGTAAAAAT TGAACCATTA GGAGTAGCAC CCACCAAGGC AAAGAGAAGA   901 GTGGTGCAGA GAGAAAAAAA GAGCAGTGGG AATAGGAGCT TTGTTCCTTG GGTTTCTTGG   961 GAGCAGCAGG AAGCACTATG GGCGCAGCGT CAATGACGCT GACGGTACAG GGCCAGACAA  1021 ATTATTGTCT GGGTATAGTG CAGCAGCAGA AACAAATTTG CTGAAGGGCT ATTTGAGGCG  1081 CAACAGGCAA TCCTGATGGC CAAACTCACA GTCTGGGGGC ATCAGGCCAA GCCTCCAGGC 1141 AGAGAAATTC CTTGGCTGTG GTTGGCA //
Primer: EF1a-N_R Region: EF-1a pro,cPPT_CTS,RRE Sequence file: RDB15736_A8Ahe.seq
>04380_15736_A9D4_EF1a-N_R_H09_08_ABI08.ab1     1 CTTTCTCTCG GGGGGGATGT GGGCGATGTG CGCTCTGCCC ACTGACGGGC ACCGGAGCCT    61 CACGAAGCTT GAATTCAAAG TGAATTTCTG CTGTCCCTGT AATAAACCCG AAAATTTTGA   121 ATTTTTGTAA TTTGTTTTTG TAATTCTTTA GTTTGTATGT CTGTTGCTAT TATGTCTACT   181 ATTCTTTCCC CTGCACTGTA CCCCCCAATC CCCCCTTTTC TTTTAAAATT GTGGATGAAT   241 ACTGCCATTT GTAGATCCGT TCACTAATCG AATGGATCTG TCTCTGTCTC TCTCTCCACC   301 TTCTTCTTCT ATTCCTTCGG GCCTGTCGGG TCCCCTCGGG GTTGGGAGGT GGGTCTGAAA   361 CGATAATGGT GAATATCCCT GCCTAACTCT ATTCACTATA GAAAGTACAG CAAAAACTAT   421 TCTTAAACCT ACCAAGCCTC CTACTATCAT TATGAATAAT TTTATATACC ACAGCCAATT   481 TGTTATGTTA AACCAATTCC ACAAACTTGC CCATTTATCT AATTCCAATA ATTCTTGTTC   541 ATTCTTTTCT TGCTGGTTTT GCGATTCTTC AATTAAGGAG TGTATTAAGC TTGTGTAATT   601 GTTAATTTCT CTGTCCCACT CCATCCAGGT CGTGTGATTC CAAATCTGTT CCAGAGATTT   661 ATTACTCCAA CTAGCATTCC AAGGCACAGC AGTGGTGCAA ATGAGTTTTC CAGAGCAAAC   721 CCCAAATCCC CAGGAGCTGT TGATCCTTTA GGTATCTTTC CACAGCCAGG ATTCTTGCCT   781 GGAGCTGCTT GATGCCCCAG ACTGTGAGTT GCAACAGATG CTGTTGCGCC TCAAATAGCC   841 CTCAGCAAAA TTGTTCTGCT GCTGCACTAT ACCAGACAAT AATTTGTCTG GCCTGGTACC   901 CGTCAGCGTC ATTTGACGCT GCGCCCCATA GTGCTTTCCT GGCTGCCTCC CCAGAAACCC   961 AAAGGAAACA AAGCTCCCTA ATTCCCACTG CTCTTTTTTT CTTCTCTGCA CCACTCTTCT  1021 CCTTGCCTTT GGTGGGGTGG CTACTCCTTA AATGGGTTCA AATCTTTTTA ACTACTTTTA  1081 TAATTTAATA TAATTCGACT TCCTCCAATT GTCCCTTCAT ATCCTCCCCG CTCCAAAGGT //
Primer: BGH_rev2 Region: bGHp(A),3'LTR,WPRE Sequence file: RDB15736_A8Ahc.seq
>04380_15736_A8Ah_BGH_rev2_E01_05_ABI08.ab1     1 CCAGTGCGGG GTCAAGGGAG GGCACGGGGG GCAGGGGCAA ACAACAGATG GCTGGCAACT    61 AGAAGGCACA GTCGAGGCTG ATCAGCGGGT TTAAACGGGC CCTGCTAGAG ATTTTCCACA   121 CTGACTAAAA GGGTCTGAGG GATCTCTAGT TACCAGAGTC ACACAACAGA CGGGCACACA   181 CTACTTGAAG CACTCAAGGC AAGCTTTATT GAGGCTTAAG CAGTGGGTTC CCTAGTTAGC   241 CAGAGAGCTC CCAGGCTCAG ATCTGGTCTA ACCAGAGAGA CCCAGTACAG TCCGGATGCA   301 GCTCTCGGGC CATGTGATGA AATGCTAGGC GGCTGTCAAA CCTCCACTCT AATACTTCTC   361 TCTCCGGGTC ATCCATCCCA TGCAGGCTCA CAGGGTGTAA CAAGCGGGTG TTCTCTCCTT   421 CATTGGCTTC TTCTACCTTC TCTTGCTCAA CTGGTACTAG CTTGTAGCAC CATCCAAAGG   481 TCAGTGGATA TCTGATCCCT GGCCCTGGTG TGTAGTTCTG CCAATCAGGG AAGTAGCCTT   541 GTGTGTGGTA GATCCACAGA TCAAGGATAT CTTGTCTTCG TTGGGAGTGA ATTAGCCCTT   601 CCAGTCCCCC CTTTTCTTTT AAAAAGTGGC TAAGATCTAC AGCTGCCTTG TAAGTCATTG   661 GTCTTAAAGG TACCTGAGGT GTGACTGGAA AACCCACCTC CTCCTCCTCT TGTGCTTCTA   721 GCCAGGCACA AATCAGCATT GGTAGCTGCT GTATTGCTAC TTGTGATTGC TCCATGTTTT   781 TCTAGGTCTC GACGGTATCG ATGCGGGGAG GCGGCCCAAA GGGAGATCCG ACTCGTCTGA   841 GGGCGAAGGC GAAGACGCGG AAGAGGCCGC AGAGCCGGCA GCAGGCCGCG GGAAGGAAGG   901 GTCCGCTGGG ATTGAGGGCC GAAGGGACGT AGCAGAAGGA CGTCCCGCGC AGAATCCAGG   961 TGGCAACACA GGCGAGCAGC CATGGAAAGG ACGTCAGCTT CCCCGACAAC ACCACGGAAA  1021 TTGTCAGTGC CCAACAGCCG AGCCCCTGTC CAGCAGCGGG CAAGGCAGGC GGCGATGAGG  1081 TTCCGGCCGT GGGCAATAGG AGGGGAAAGC GAAGTTCCCC GGGAAAAGAA GCTGACCGTG 1141 TTGGCATGGC CCAACAAGTG GGGGGTTTGC CGTCCAGGCC AAAACCACCA AGTGTG //
Primer: SV40pro_F Region: SV40pro_ori,Zeo,SV40p(A) Sequence file: RDB15736_A8Ahd.seq
>04380_15736_A8Ah_SV40pro_F_E01_05_ABI08.ab1     1 CCCTGGTGGG GGTGGCTTTC AGAAGTAGTG GAGGAGGCTT TTTTGGAGGC CTAGGCTTTT    61 GCAAAAAGCT CCCGGGAGCT TGTATATCCA TTTTCGGATC TGATCAGCAC GTGTTGACAA   121 TTAATCATCG GCATAGTATA TCGGCATAGT ATAATACGAC AAGGTGAGGA ACTAAACCAT   181 GGCCAAGTTG ACCAGTGCCG TTCCGGTGCT CACCGCGCGC GACGTCGCCG GAGCGGTCGA   241 GTTCTGGACC GACCGGCTCG GGTTCTCCCG GGACTTCGTG GAGGACGACT TCGCCGGTGT   301 GGTCCGGGAC GACGTGACCC TGTTCATCAG CGCGGTCCAG GACCAGGTGG TGCCGGACAA   361 CACCCTGGCC TGGGTGTGGG TGCGCGGCCT GGACGAGCTG TACGCCGAGT GGTCGGAGGT   421 CGTGTCCACG AACTTCCGGG ACGCCTCCGG GCCGGCCATG ACCGAGATCG GCGAGCAGCC   481 GTGGGGGCGG GAGTTCGCCC TGCGCGACCC GGCCGGCAAC TGCGTGCACT TCGTGGCCGA   541 GGAGCAGGAC TGACACGTGC TACGAGATTT CGATTCCACC GCCGCCTTCT ATGAAAGGTT   601 GGGCTTCGGA ATCGTTTTCC GGGACGCCGG CTGGATGATC CTCCAGCGCG GGGATCTCAT   661 GCTGGAGTTC TTCGCCCACC CCAACTTGTT TATTGCAGCT TATAATGGTT ACAAATAAAG   721 CAATAGCATC ACAAATTTCA CAAATAAAGC ATTTTTTTCA CTGCATTCTA GTTGTGGTTT   781 GTCCAAACTC ATCAATGTAT CTTATCATGT CTGTATACCG TCGACCTCTA GCTAGAGCTT   841 GGCGTAATCA TGGTCATAGC TGTTTCCTGT GTGAAATTGT TATCCGCTCA CAATTCCACA   901 CAACATACGA GCCGGAAGCA TAAAGTGTAA AGCCTGGGGG TGCCTAATGA GTGAGCTAAC   961 TCACATTAAT TGCGTTGCGC TCACTGCCCG CTTTCCAGTC GGGAAACCCT GTCGTGCCAG  1021 CTGCATTAAA TGGAATCGGC CAACGCGCGG GGAGAGGCGG TTTGCGTAAT GGCGGCTCTT  1081 CGGCTTCTCG CTTCACTGAC TTCGCTGCGC TCGATCGTTC CGCCTGGCGG CGAGCGGTAT 1141 CAGCTTCACC TTCAAAAAGG GGCGCGGGTA GAT //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2022.07.13

GNP_filter3_RDBDEP_html_220713.pl