Resource data sheet
DNA Bank Top

CS-CDF-EG-PRE (#RDB04380)

Lentivirus vector plasmid of GFP driven by human EF-1 alpha promoter.


Drawn by SnapGene® software
Sequence (full) RDB04380hts01.seq
Remarks, protocol and/or map (pdf) RDB04380.pdf
Publication Kim, S., Stem Cells Dev. 16 (4): 537-545 (2007). [link to RRC of NBRP]
Test sheet RDB18955_B3Kap1-2.pdf 
Assembled from experimentally sequenced data.
 
Clone info. Lentivirus vector plasmid of GFP driven by human EF-1 alpha promoter.
Comment Commonly requested with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394).
Vector backbone Plasmid
Selectable markers Ampicillin (E. coli). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Growth conditions 37C, LB+Amp
Depositor|Developer Miyoshi, Hiroyuki |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose)[Word] [Example of MTA ]
Please visit Ordering instruction.[link] 
Terms and conditions for distribution The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer.
Remarks ((Remarks)) Remember that you will be working with samples containing infectious virus.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利機関による非営利学術研究用)[Word] [MTAの記入例 ]
遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。
手続きの概要は、「レンチウイルスベクターの提供申し込み」をご覧ください。
MTAに書く使用条件 本件研究材料は、非営利機関の非営利学術研究に限って提供する。本件研究材料を利用した研究結果等を発表する際は、本件研究材料が故三好浩之博士により開発されたことを明示する。
備考 【使用上の注意】このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB04380 CS-CDF-EG-PRE DNA solution

Ordering instruction of plasmids [in Japanese] [in English]

How to cite this biological resource

Materials & Methods section:

The CS-CDF-EG-PRE was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB04380).

Reference section:

Kim, S., Kim, G.J., Miyoshi, H., Moon, S.H., Ahn, S.E., Lee, J.H., Lee, H.J., Cha, K.Y., Chung, H.M., Efficiency of the elongation factor-1alpha promoter in mammalian embryonic stem cells using lentiviral gene delivery systems. Stem Cells Dev. 16 (4): 537-545 (2007). PMID 17784828. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Kim, S., Efficiency of the elongation factor-1alpha promoter in mammalian embryonic stem cells using lentiviral gene delivery systems. Stem Cells Dev. 16 (4): 537-545 (2007). PMID 17784828. [link to RRC of NBRP]
user_report Inagaki, S., Genetically encoded bioluminescent voltage indicator for multi-purpose use in wide range of bioimaging. Sci. Rep. 7: 42398 (2017). PMID 28205521. [link to RRC of NBRP]
user_report Yaguchi, M., Characterization of the properties of seven promoters in the motor cortex of rats and monkeys after lentiviral vector-mediated gene transfer. Hum. Gene Ther. Methods, 24 (6): 333-344 (2013). PMID 23964981. [link to RRC of NBRP]