Resource data sheet
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CS-CDF-EG-PRE

Lentivirus vector construct of GFP driven by human EF-1 alpha promoter.

Catalog number RDB04380
Resource name CS-CDF-EG-PRE
Clone info. Lentivirus vector construct of GFP driven by human EF-1 alpha promoter
Commonly requested with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Vector backbone Plasmid
Selectable markers Amp^r (E. coli); Zeo^r (plasmid transfection of mammalian cells).
Originator Miyoshi, Hiroyuki |

Distribution information

CLICK here for plasmid list and map. [link]
Forms for plasmid request [in Japanese] [in English]
Terms and conditions for distribution The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose.
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Remarks ((Remarks)) Remember that you will be working with samples containing infectious virus.
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Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB15736_A8Ahp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: EF1a-C_F RDB15736_A8Aha.seq
>04380_15736_A8Ah_EF1a-C_F_D01_04_ABI08.ab1
    1 GCCTTTTTGG ATTTGCCCTT TTTGAGTTTG GATCTTGGTT CATTCTCAAG CCTCAGACAG
   61 TGGTTCAAAG TTTTTTTCTT CCATTTCAGG TGTCGTGAAC ACGCTACCGG TCGCCACCAT
  121 GGTGAGCAAG GGCGAGGAGC TGTTCACCGG GGTGGTGCCC ATCCTGGTCG AGCTGGACGG
  181 CGACGTAAAC GGCCACAAGT TCAGCGTGTC CGGCGAGGGC GAGGGCGATG CCACCTACGG
  241 CAAGCTGACC CTGAAGTTCA TCTGCACCAC CGGCAAGCTG CCCGTGCCCT GGCCCACCCT
  301 CGTGACCACC CTGACCTACG GCGTGCAGTG CTTCAGCCGC TACCCCGACC ACATGAAGCA
  361 GCACGACTTC TTCAAGTCCG CCATGCCCGA AGGCTACGTC CAGGAGCGCA CCATCTTCTT
  421 CAAGGACGAC GGCAACTACA AGACCCGCGC CGAGGTGAAG TTCGAGGGCG ACACCCTGGT
  481 GAACCGCATC GAGCTGAAGG GCATCGACTT CAAGGAGGAC GGCAACATCC TGGGGCACAA
  541 GCTGGAGTAC AACTACAACA GCCACAACGT CTATATCATG GCCGACAAGC AGAAGAACGG
  601 CATCAAGGTG AACTTCAAGA TCCGCCACAA CATCGAGGAC GGCAGCGTGC AGCTCGCCGA
  661 CCACTACCAG CAGAACACCC CCATCGGCGA CGGCCCCGTG CTGCTGCCCG ACAACCACTA
  721 CCTGAGCACC CAGTCCGCCC TGAGCAAAGA CCCCAACGAG AAGCGCGATC ACATGGTCCT
  781 GCTGGAGTTC GTGACCGCCG CCGGGATCAC TCTCGGCATG GACGAGCTGT ACAAGTCCGG
  841 ACTCAGATCC TACTAGTAGG ATCTCGAGGG ATCAAGCTTA TCGATAATCA ACCTCTGGAT
  901 TACAAAATTT GTGAAAGATT GACTGGTATT CTTAACTATG TTGCTCCTTT TTACGCTATG
  961 TGGATACGCT GCTTTAATGC CTTTTGTATC ATGCTATTGC TTCCCGTATG GCTTTCATTT
 1021 TTCTCCTTCC TTTGTATAAA TTCCTATTTG CTGTCTCTTT ATGAGGAGTT GTGGCCCGTT
 1081 GTCAGGCAAC GTGCGTGGTG TGCCACTGTG TTGCTGGACG CAACCCTCTC ACTTGGCTGG
1141 GGCATTGGCC AACACTGCTC AGCTTCTTTA CCGGAAACCA T
//
Primer: CMV-Forward RDB15736_A8Ahb.seq
>04380_15736_A8Ah_CMV-Forward_C01_03_ABI08.ab1
    1 CTGTTAAAAC TTCCGCCCCA TTGACGCAAA TGGGCGGTAG GCGTGTACGG TGGGAGGTCT
   61 ATATAAGCAG CGCGTTTTGC CTGTACTGGG TCTCTCTGGT TAGACCAGAT CTGAGCCTGG
  121 GAGCTCTCTG GCTAACTAGG GAACCCACTG CTTAAGCCTC AATAAAGCTT GCCTTGAGTG
  181 CTTCAAGTAG TGTGTGCCCG TCTGTTGTGT GACTCTGGTA ACTAGAGATC CCTCAGACCC
  241 TTTTAGTCAG TGTGGAAAAT CTCTAGCAGT GGCGCCCGAA CAGGGACTTG AAAGCGAAAG
  301 GGAAACCAGA GGAGCTCTCT CGACGCAGGA CTCGGCTTGC TGAAGCGCGC ACGGCAAGAG
  361 GCGAGGGGCG GCGACTGGTG AGTACGCCAA AAATTTTGAC TAGCGGAGGC TAGAAGGAGA
  421 GAGATGGGTG CGAGAGCGTC AGTATTAAGC GGGGGAGAAT TAGATCGCGA TGGGAAAAAA
  481 TTCGGTTAAG GCCAGGGGGA AAGAAAAAAT ATAAATTAAA ACATATAGTA TGGGCAAGCA
  541 GGGAGCTAGA ACGATTCGCA GTTAATCCTG GCCTGTTAGA AACATCAGAA GGCTGTAGAC
  601 AAATACTGGG ACAGCTACAA CCATCCCTTC AGACAGGATC AGAAGAACTT AGATCATTAT
  661 ATAATACAGT AGCAACCCTC TATTGTGTGC ATCAAAGGAT AGAGATAAAA GACACCAAGG
  721 AAGCTTTAGA CAAGATAGAG GAAGAGCAAA ACAAAAGTAA GACCACCGCA CAGCAAGCGG
  781 CCGCTGATCT TCAGACCTGG AGGAGGAGAT ATGAGGGACA ATTGGAGAAG TGAATTATAT
  841 AAATATAAAG TAGTAAAAAT TGAACCATTA GGAGTAGCAC CCACCAAGGC AAAGAGAAGA
  901 GTGGTGCAGA GAGAAAAAAA GAGCAGTGGG AATAGGAGCT TTGTTCCTTG GGTTTCTTGG
  961 GAGCAGCAGG AAGCACTATG GGCGCAGCGT CAATGACGCT GACGGTACAG GGCCAGACAA
 1021 ATTATTGTCT GGGTATAGTG CAGCAGCAGA AACAAATTTG CTGAAGGGCT ATTTGAGGCG
 1081 CAACAGGCAA TCCTGATGGC CAAACTCACA GTCTGGGGGC ATCAGGCCAA GCCTCCAGGC
1141 AGAGAAATTC CTTGGCTGTG GTTGGCA
//
Primer: BGH_rev2 RDB15736_A8Ahc.seq
>04380_15736_A8Ah_BGH_rev2_E01_05_ABI08.ab1
    1 CCAGTGCGGG GTCAAGGGAG GGCACGGGGG GCAGGGGCAA ACAACAGATG GCTGGCAACT
   61 AGAAGGCACA GTCGAGGCTG ATCAGCGGGT TTAAACGGGC CCTGCTAGAG ATTTTCCACA
  121 CTGACTAAAA GGGTCTGAGG GATCTCTAGT TACCAGAGTC ACACAACAGA CGGGCACACA
  181 CTACTTGAAG CACTCAAGGC AAGCTTTATT GAGGCTTAAG CAGTGGGTTC CCTAGTTAGC
  241 CAGAGAGCTC CCAGGCTCAG ATCTGGTCTA ACCAGAGAGA CCCAGTACAG TCCGGATGCA
  301 GCTCTCGGGC CATGTGATGA AATGCTAGGC GGCTGTCAAA CCTCCACTCT AATACTTCTC
  361 TCTCCGGGTC ATCCATCCCA TGCAGGCTCA CAGGGTGTAA CAAGCGGGTG TTCTCTCCTT
  421 CATTGGCTTC TTCTACCTTC TCTTGCTCAA CTGGTACTAG CTTGTAGCAC CATCCAAAGG
  481 TCAGTGGATA TCTGATCCCT GGCCCTGGTG TGTAGTTCTG CCAATCAGGG AAGTAGCCTT
  541 GTGTGTGGTA GATCCACAGA TCAAGGATAT CTTGTCTTCG TTGGGAGTGA ATTAGCCCTT
  601 CCAGTCCCCC CTTTTCTTTT AAAAAGTGGC TAAGATCTAC AGCTGCCTTG TAAGTCATTG
  661 GTCTTAAAGG TACCTGAGGT GTGACTGGAA AACCCACCTC CTCCTCCTCT TGTGCTTCTA
  721 GCCAGGCACA AATCAGCATT GGTAGCTGCT GTATTGCTAC TTGTGATTGC TCCATGTTTT
  781 TCTAGGTCTC GACGGTATCG ATGCGGGGAG GCGGCCCAAA GGGAGATCCG ACTCGTCTGA
  841 GGGCGAAGGC GAAGACGCGG AAGAGGCCGC AGAGCCGGCA GCAGGCCGCG GGAAGGAAGG
  901 GTCCGCTGGG ATTGAGGGCC GAAGGGACGT AGCAGAAGGA CGTCCCGCGC AGAATCCAGG
  961 TGGCAACACA GGCGAGCAGC CATGGAAAGG ACGTCAGCTT CCCCGACAAC ACCACGGAAA
 1021 TTGTCAGTGC CCAACAGCCG AGCCCCTGTC CAGCAGCGGG CAAGGCAGGC GGCGATGAGG
 1081 TTCCGGCCGT GGGCAATAGG AGGGGAAAGC GAAGTTCCCC GGGAAAAGAA GCTGACCGTG
1141 TTGGCATGGC CCAACAAGTG GGGGGTTTGC CGTCCAGGCC AAAACCACCA AGTGTG
//
Primer: SV40pro_F RDB15736_A8Ahd.seq
>04380_15736_A8Ah_SV40pro_F_E01_05_ABI08.ab1
    1 CCCTGGTGGG GGTGGCTTTC AGAAGTAGTG GAGGAGGCTT TTTTGGAGGC CTAGGCTTTT
   61 GCAAAAAGCT CCCGGGAGCT TGTATATCCA TTTTCGGATC TGATCAGCAC GTGTTGACAA
  121 TTAATCATCG GCATAGTATA TCGGCATAGT ATAATACGAC AAGGTGAGGA ACTAAACCAT
  181 GGCCAAGTTG ACCAGTGCCG TTCCGGTGCT CACCGCGCGC GACGTCGCCG GAGCGGTCGA
  241 GTTCTGGACC GACCGGCTCG GGTTCTCCCG GGACTTCGTG GAGGACGACT TCGCCGGTGT
  301 GGTCCGGGAC GACGTGACCC TGTTCATCAG CGCGGTCCAG GACCAGGTGG TGCCGGACAA
  361 CACCCTGGCC TGGGTGTGGG TGCGCGGCCT GGACGAGCTG TACGCCGAGT GGTCGGAGGT
  421 CGTGTCCACG AACTTCCGGG ACGCCTCCGG GCCGGCCATG ACCGAGATCG GCGAGCAGCC
  481 GTGGGGGCGG GAGTTCGCCC TGCGCGACCC GGCCGGCAAC TGCGTGCACT TCGTGGCCGA
  541 GGAGCAGGAC TGACACGTGC TACGAGATTT CGATTCCACC GCCGCCTTCT ATGAAAGGTT
  601 GGGCTTCGGA ATCGTTTTCC GGGACGCCGG CTGGATGATC CTCCAGCGCG GGGATCTCAT
  661 GCTGGAGTTC TTCGCCCACC CCAACTTGTT TATTGCAGCT TATAATGGTT ACAAATAAAG
  721 CAATAGCATC ACAAATTTCA CAAATAAAGC ATTTTTTTCA CTGCATTCTA GTTGTGGTTT
  781 GTCCAAACTC ATCAATGTAT CTTATCATGT CTGTATACCG TCGACCTCTA GCTAGAGCTT
  841 GGCGTAATCA TGGTCATAGC TGTTTCCTGT GTGAAATTGT TATCCGCTCA CAATTCCACA
  901 CAACATACGA GCCGGAAGCA TAAAGTGTAA AGCCTGGGGG TGCCTAATGA GTGAGCTAAC
  961 TCACATTAAT TGCGTTGCGC TCACTGCCCG CTTTCCAGTC GGGAAACCCT GTCGTGCCAG
 1021 CTGCATTAAA TGGAATCGGC CAACGCGCGG GGAGAGGCGG TTTGCGTAAT GGCGGCTCTT
 1081 CGGCTTCTCG CTTCACTGAC TTCGCTGCGC TCGATCGTTC CGCCTGGCGG CGAGCGGTAT
1141 CAGCTTCACC TTCAAAAAGG GGCGCGGGTA GAT
//

Please visit Sequencing and PCR primers for primer information.

References and tips

Electronic file

References

original Kim, S., Efficiency of the elongation factor-1alpha promoter in mammalian embryonic stem cells using lentiviral gene delivery systems. Stem Cells Dev. 16 (4): 537-545 (2007). PMID 17784828.
user report Inagaki, S., Genetically encoded bioluminescent voltage indicator for multi-purpose use in wide range of bioimaging. Sci. Rep. 7: 42398 (2017). PMID 28205521.
user report Yaguchi, M., Characterization of the properties of seven promoters in the motor cortex of rats and monkeys after lentiviral vector-mediated gene transfer. Hum. Gene Ther. Methods, 24 (6): 333-344 (2013). PMID 23964981.

2018.03.15

GNP_filter3_RDBDEP_html_171214.pl