CS-CDF-CG-PRE (#RDB04379)
Lentivirus vector plasmid of GFP driven by CMV promoter.
 Drawn by SnapGene® software |
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| Clone info. | Lentivirus vector plasmid of GFP driven by CMV promoter. |
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| Comment | pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394) are popular choices for lentivirus packaging. |
| Vector backbone | Plasmid |
| Selectable markers | Ampicillin (E. coli). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector. |
| Growth conditions | 37℃, LB+Amp |
| Depositor|Developer | Miyoshi, Hiroyuki | |
Distribution information
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
| Ordering forms | Order form [Credit Card Payment  ] [Bank Transfer Payment] [Example of order form ]MTA, for use for not-for-profit academic purpose [Word ] [Example of MTA ]Please visit Ordering instruction.[link] |
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| Terms and conditions for distribution | The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer. |
| Remarks | ((Remarks)) Remember that you will be working with samples containing infectious virus. |
[open/close]| 必要書類 | 提供依頼書 [依頼書の記入例 ]提供同意書 (MTA, 非営利学術目的用)[Word ] [MTAの記入例 ]手続きの概要は、「レンチウイルスベクターの提供申し込み」をご覧ください。 |
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| MTAに書く使用条件 | 本件研究材料は、非営利機関の非営利学術研究に限って提供する。本件研究材料を利用した研究結果等を発表する際は、本件研究材料が故三好浩之博士により開発されたことを明示する。 |
| 備考 | 【使用上の注意】このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。 |
| Catalog # | Resource name | Shipping form | Fee |
|---|---|---|---|
| RDB04379 | CS-CDF-CG-PRE | DNA solution |
How to cite this biological resource
Materials & Methods section:
| The CS-CDF-CG-PRE was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB04379). |
Reference section:
| Sumimoto, H., Tsuji, T., Miyoshi, H., Hagihara, M., Takada-Yamazaki, R., Okamoto, S., Ikeda, Y., Takahashi, T., Kawakami, Y., Rapid and efficient generation of lentivirally gene-modified dendritic cells from DC progenitors with bone marrow stromal cells. J. Immunol. Methods 271 (1-2): 153-165 (2002). PMID 12445738. [PubMed] [Article] [RRC of NBRP] |
Further references such as user reports and related articles (go to bottom)
References
Original, user report and related articles
| original | Sumimoto, H., Rapid and efficient generation of lentivirally gene-modified dendritic cells from DC progenitors with bone marrow stromal cells. J. Immunol. Methods 271 (1-2): 153-165 (2002). PMID 12445738. [PubMed] [Article] [RRC of NBRP] |
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| reference | Yamaguchi, T., Comparison of gene-trapping efficiency between retroviral and lentiviral vectors in mouse embryonic stem cells. Biochem. Biophys. Res. Commun. 425 (2): 297-303 (2012). PMID 22842569. [PubMed] [Article] [RRC of NBRP] |
| reference | Shibuya, K., CD226 (DNAM-1) is involved in lymphocyte function-associated antigen 1 costimulatory signal for naive T cell differentiation and proliferation. J. Exp. Med. 198 (12): 1829-1839 (2003). PMID 14676297. [PubMed] [Article] [RRC of NBRP] |
| reference | Tahara-Hanaoka, S., Lentiviral vector-mediated transduction of murine CD34(-) hematopoietic stem cells. Exp. Hematol. 30 (1): 11-17 (2002). PMID 11823032. [PubMed] [Article] [RRC of NBRP] |
| reference | Miyoshi, H., Development of a self-inactivating lentivirus vector. J. Virol., 72 (10): 8150-8157 (1998). PMID 9733856. [PubMed] [Article] [RRC of NBRP] |
| user_report | Honda, A., Very-long-chain fatty acids are crucial to neuronal polarity by providing sphingolipids to lipid rafts. Cell Rep. 42 (10): 113195 (2023). PMID 37816355. [PubMed] [Article] [RRC of NBRP] |
| user_report | Hachiya, K., Obesity-induced PARIS (ZNF746) accumulation in adipose progenitor cells leads to attenuated mitochondrial biogenesis and impaired adipogenesis. Sci. Rep. 13 (1): 22990 (2023). PMID 38151567. [PubMed] [Article] [RRC of NBRP] |
| user_report | Miyazaki, S., Establishment of a long-term stable beta-cell line and its application to analyze the effect of Gcg expression on insulin secretion. Sci. Rep. 11 (1): 477 (2021). PMID 33436850. [PubMed] [Article] [RRC of NBRP] |
| user_report | Murayama, A.Y., The polymicrogyria-associated GPR56 promoter preferentially drives gene expression in developing GABAergic neurons in common marmosets. Sci. Rep. 10 (1): 21516 (2020). PMID 33299078. [PubMed] [Article] [RRC of NBRP] |
| user_report | Suzuki, E., Secretion of high amounts of hepatocyte growth factor is a characteristic feature of cancer-associated fibroblasts with EGFR-TKI resistance-promoting phenotype: A study of 18 cases of cancer-associated fibroblasts. Pathol. Int. 69 (8): 472-480 (2019). PMID 31339210. [PubMed] [Article] [RRC of NBRP] |
| user_report | Morikawa, M., The ALK-1/SMAD/ATOH8 axis attenuates hypoxic responses and protects against the development of pulmonary arterial hypertension. Sci. Signal. 12(607). pii: eaay4430 (2019). PMID 31719172. [PubMed] [Article] [RRC of NBRP] |
| user_report | Ogawa, Y., Elavl3 is essential for the maintenance of Purkinje neuron axons. Sci. Rep. 8 (1): 2722 (2018). PMID 29426875. [PubMed] [Article] [RRC of NBRP] |
| user_report | Shamoto, N., CFAP70 Is a Novel Axoneme-Binding Protein That Localizes at the Base of the Outer Dynein Arm and Regulates Ciliary Motility. Cells 7 (9). pii: E124 (2018). PMID 30158508. [PubMed] [Article] [RRC of NBRP] |
| user_report | Andharia, N., Involvement of intracellular transport in TREK-1c current run-up in 293T cells. Channels (Austin) 11 (3): 224-235 (2017). PMID 28085542. [PubMed] [Article] [RRC of NBRP] |
| user_report | Katsura, A., ZEB1-regulated inflammatory phenotype in breast cancer cells. Mol. Oncol. 11 (9): 1241-1262 (2017). PMID 28618162. [PubMed] [Article] [RRC of NBRP] |
| user_report | Kubota, S.I., Whole-Body Profiling of Cancer Metastasis with Single-Cell Resolution. Cell Rep. 20 (1): 236-250 (2017). PMID 28683317. [PubMed] [Article] [RRC of NBRP] |
| user_report | Kobayashi, M., Functional Analysis of Novel Candidate Regulators of Insulin Secretion in the MIN6 Mouse Pancreatic beta Cell Line PLoS One 11 (3): e0151927 (2016). PMID 26986842. [PubMed] [Article] [RRC of NBRP] |
| user_report | Suzuki, S., Characterization of actions of octanoate on porcine preadipocytes and adipocytes differentiated in vitro. Biochem. Biophys. Res. Commun., 432 (1): 92-98 (2013). PMID 23376076. [PubMed] [Article] [RRC of NBRP] |
| user_report | Yaguchi, M., Characterization of the properties of seven promoters in the motor cortex of rats and monkeys after lentiviral vector-mediated gene transfer. Hum. Gene Ther. Methods, 24 (6): 333-344 (2013). PMID 23964981. [PubMed] [Article] [RRC of NBRP] |
| user_report | Tamura, K., A glass-coated tungsten microelectrode enclosing optical fibers for optogenetic exploration in primate deep brain structures. J. Neurosci. Methods, 211 (1): 49-57 (2012). PMID 22971353. [PubMed] [Article] [RRC of NBRP] |
| user_report | Saitoh, Y., Overexpression of NF-kappaB inducing kinase underlies constitutive NF-kappaB activation in lung cancer cells. Lung. Cancer., 70, 263-270 (2010). PMID 20338663. [PubMed] [Article] [RRC of NBRP] |
| user_report | Ohashi Y., A bicistronic lentiviral vector-based method for differential transsynaptic tracing of neural circuits. Mol. Cell. Neurosci. 46 (1): 136-147. PMID 20816792. [PubMed] [Article] [RRC of NBRP] |
| user_report | Hanyu, A., Functional in vivo optical imaging of tumor angiogenesis, growth, and metastasis prevented by administration of anti-human VEGF antibody in xenograft model of human fibrosarcoma HT1080 cells. Cancer Sci. 100 (11): 2085-2092 (2009). PMID 19719773. [PubMed] [Article] [RRC of NBRP] |
| user_report | Yoshida, S., Knockdown of synapse-associated protein Dlg1 reduces syncytium formation induced by human T-cell leukemia virus type 1. Virus Genes, 37 (1): 9-15 (2008). PMID 18461433. [PubMed] [Article] [RRC of NBRP] |
| user_report | Inoue-Narita, T., Pten deficiency in melanocytes results in resistance to hair graying and susceptibility to carcinogen-induced melanomagenesis. Cancer Res. 2008 Jul 15;68(14):5760-8 (2008). PMID 18632629. [PubMed] [Article] [RRC of NBRP] |
| user_report | Ehata, S., Ki26894, a novel transforming growth factor-beta type I receptor kinase inhibitor, inhibits in vitro invasion and in vivo bone metastasis of a human breast cancer cell line. Cancer Sci. 98 (1): 127-133 (2007). PMID 17129361. [PubMed] [Article] [RRC of NBRP] |
| user_report | Lu F.Z., Long-term gene expression using the lentiviral vector in rat chondrocytes. Clin. Orthop. Relat. Res., 439, 243-252 (2005). PMID 16205166. [PubMed] [Article] [RRC of NBRP] |
| user_report | Lu F.Z., Characterization and gene transfer in mesenchymal stem cells derived from human umbilical-cord blood. J. Lab. Clin. Med., 146, 271-278 (2005). PMID 16242526. [PubMed] [Article] [RRC of NBRP] |
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