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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

CS-CDF-CG-PRE

Lentivirus vector plasmid of GFP driven by CMV promoter.

Catalog number RDB04379
Resource name CS-CDF-CG-PRE
Clone info. Lentivirus vector plasmid of GFP driven by CMV promoter.
Comment Commonly requested with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Vector backbone Plasmid
Selectable markers Please note that Zeo resistance marker is not included in the lentivirus produced from this vector. E. coli: Amp^r.
Depositor|Developer Miyoshi, Hiroyuki |
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External Database

            Reference sequence
              
            Lentiviral plasmid list. [link]

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer.
            Ordering Please visit Ordering instruction.[link] 
            Order form 
            [Credit Card Payment]  [Bank Transfer Payment]
            Material Transfer Agreement (MTA, for use for not-for-profit academic purpose)[Word]
            This RESOURCE contains a fluorescent protein owned or licensed by Global Life Sciences Solutions Operations UK Ltd. (Cytiva). Please refer to Schedule B and use Schedule A (download the Schedule A and Schedule B [link]).
            Remarks ((Remarks)) Remember that you will be working with samples containing infectious virus.
            提供案内 (日本国内) [open/close]

            提供条件 本件研究材料は、非営利機関の非営利学術研究に限って提供する。本件研究材料を利用した研究結果等を発表する際は、本件研究材料が故三好浩之博士により開発されたことを明示する。
            提供依頼 手続きの詳細は、「レンチウイルスベクターの提供申し込み」をご覧ください。
            提供依頼書 [Word]
            提供同意書 (非営利機関による非営利学術研究用)[Word]
            GFPリソースを含みます。Schedule Bをご一読の上Schedule A (英語版のみ) の提出をお願いします (Schedule AとSchedule Bを入手[link])。
            遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。
            備考 【使用上の注意】このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB04379 CS-CDF-CG-PRE DNA solution

            Ordering instruction of plasmids [in Japanese] [in English]
            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Electronic file

            Sequence RDB04379z.seq
            Remarks, protocol and/or map (pdf) RDB04379.pdf

            Original reference

            original Shibuya, K., CD226 (DNAM-1) is involved in lymphocyte function-associated antigen 1 costimulatory signal for naive T cell differentiation and proliferation. J. Exp. Med. 198 (12): 1829-1839 (2003). PMID 14676297.
            original Tahara-Hanaoka, S., Lentiviral vector-mediated transduction of murine CD34(-) hematopoietic stem cells. Exp. Hematol. 30 (1): 11-17 (2002). PMID 11823032.
            original Sumimoto, H., Rapid and efficient generation of lentivirally gene-modified dendritic cells from DC progenitors with bone marrow stromal cells. J. Immunol. Methods 271 (1-2): 153-165 (2002). PMID 12445738.
            original Miyoshi, H., Development of a self-inactivating lentivirus vector. J. Virol., 72 (10): 8150-8157 (1998). PMID 9733856.

            Further references such as user reports and related articles (go to bottom)

            Featured content


            Sequence information

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB17487_A9I6p1-1.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: EGFP-M_R
            Region: EGFP,CMV pro
            Sequence file: RDB17487_A9I6e.seq check
            >04379_17487_A9I6_3_EGFP-M_R_A01_01_ABI08.ab1
                1 NNNNNNNNNN NNNNNNNNNN NNNGGNNCTT GNNNTTNNCC TTGNTGCCGT TCTTCTGCTT
               61 GTCGGCCATG ATATANNNNT TGTGGCTGTT GTAGTTGTAC TCCAGCTTGT GCCCCAGGAT
              121 GTTGCCGTCC TCCTTGAAGT CGATGCCCTT CAGCTCGATG CGGTTCACCA GGGTGTCGCC
              181 CTCGAACTTC ACCTCGGCGC GGGTCTTGTA GTTGCCGTCG TCCTTGAAGA AGATGGTGCG
              241 CTCCTGGACG TAGCCTTCGG GCATGGCGGA CTTGAAGAAG TCGTGCTGCT TCATGTGGTC
              301 GGGGTAGCGG CTGAAGCACT GCACGCCGTA GGTCAGGGTG GTCACGAGGG TGGGCCAGGG
              361 CACGGGCAGC TTGCCGGTGG TGCAGATGAA CTTCAGGGTC AGCTTGCCGT AGGTGGCATC
              421 GCCCTCGCCC TCGCCGGACA CGCTGAACTT GTGGCCGTTT ACGTCGCCGT CCAGCTCGAC
              481 CAGGATGGGC ACCACCCCGG TGAACAGCTC CTCGCCCTTG CTCACCATGG TGGCGACCGG
              541 TAGCGCTAGC GGATCTGACG GTTCACTAAA CCAGCTCTGC TTATATAGAC CTCCCACCGT
              601 ACACGCCTAC CGCCCATTTG CGTCAATGGG GCGGAGTTGT TACGACATTT TGGAAAGTCC
              661 CGTTGATTTT GGTGCCAAAA CAAACTCCCA TTGACGTCAA TGGGGTGGAG ACTTGGAAAT
              721 CCCCGTGAGT CAAACCGCTA TCCACGCCCA TTGATGTACT GCCAAAACCG CATCANCATG
              781 GTAATAGCGA TGACTAATAC GTANATGTAC TGCCAAGTAN GAAAGTCCCA TAANGTCATG
              841 TACTGGGCAT AATGCNAGGC GGGCCATTTA NCGTCNTTGA CGTCNATAGG GGGCGTACTT
            901 GGNCATATGA TACNCTTGAT GTACTGCC
            //
            Primer: PRE_rev
            Region: PRE,EGFP
            Sequence file: RDB17487_A9I6f.seq check
            >04379_17487_A9I6_3_PRE_rev_B10_04_ABI24.ab1
                1 NNNNNNNNNN NNNNNNNNNN NNNNANCNNN ATAGTTAAGA ATACCAGTCA ATCTTTCNCA
               61 AATTTTGTAA TCCAGAGGTT GATTATCGAT AAGCTTGATC CCTCGAGATC CTACTAGTAG
              121 GATCTGAGTC CGGACTTGTA CAGCTCGTCC ATGCCGAGAG TGATCCCGGC GGCGGTCACG
              181 AACTCCAGCA GGACCATGTG ATCGCGCTTC TCGTTGGGGT CTTTGCTCAG GGCGGACTGG
              241 GTGCTCAGGT AGTGGTTGTC GGGCAGCAGC ACGGGGCCGT CGCCGATGGG GGTGTTCTGC
              301 TGGTAGTGGT CGGCGAGCTG CACGCTGCCG TCCTCGATGT TGTGGCGGAT CTTGAAGTTC
              361 ACCTTGATGC CGTTCTTCTG CTTGTCGGCC ATGATATAGA CGTTGTGGCT GTTGTAGTTG
              421 TACTCCAGCT TGTGCCCCAG GATGTTGCCG TCCTCCTTGA AGTCGATGCC CTTCAGCTCG
              481 ATGCGGTTCA CCAGGGTGTC GCCCTCGAAC TTCACCTCGG CGCGGGTCTT GTAGTTGCCG
              541 TCGTCCTTGA AGAAGATGGT GCGCTCCTGG ACGTAGCCTT CGGGCATGGC GGACTTGAAG
              601 AAGTCGTGCT GCTTCATGTG GTCGGGGTAG CGGCTGAAGC ACTGCACGCC GTAGGTCANG
              661 GTGGTCACGA GGGTGGGCCA GGGCACGGGC AGCTTGCCGG TGGTGCAGAT GAACTTCANG
              721 GTCAGCTTGC CGTANGTGGC ATCGCCCTCG CCCTCGCCGG ACACGCTGAA CTTGTGGCCG
              781 TTTACGTCGC CGTCCAGCTC GACCAGGATG GGCACCACCC CGGTGAACAG CTCCTCGCCC
            841 TTGCTCACCA TGGTGGCGAC CGGTAGCGCT AGCGGATCTG ACGGTTCACT NANN
            //
            Primer: HIV-B_R
            Region: HIV-1psi,5'LTR,CMVpro
            Sequence file: RDB17487_A9I6g.seq check
            >04379_17487_A9I6_3_HIV-B_R_C10_07_ABI24.ab1
                1 NNNNNNNNNN NNNNCNNNNN NNNNTTTTGG NGTACTCNCC NGTCGCCGCC CCTCGCCTCT
               61 TGCCGTGCGC GCTTCAGCAA GCCGAGTCCT GCGTCGAGAG AGCTCCTCTG GTTTCCCTTT
              121 CGCTTTCAAG TCCCTGTTCG GGCGCCACTG CTAGAGATTT TCCACACTGA CTAAAAGGGT
              181 CTGAGGGATC TCTAGTTACC AGAGTCACAC AACAGACGGG CACACACTAC TTGAAGCACT
              241 CAAGGCAAGC TTTATTGAGG CTTAAGCAGT GGGTTCCCTA GTTAGCCAGA GAGCTCCCAG
              301 GCTCAGATCT GGTCTAACCA GAGAGACCCA GTACAGGCAA AACGCGCTGC TTATATAGAC
              361 CTCCCACCGT ACACGCCTAC CGCCCATTTG CGTCAATGGG GCGGAGTTGT TACGACATTT
              421 TGGAAAGTCC CGTTGATTTT GGTGCCAAAA CAAACTCCCA TTGACGTCAA TGGGGTGGAG
              481 ACTTGGAAAT CCCCGTGAGT CAAACCGCTA TCCACGCCCA TTGATGTACT GCCAAAACCG
              541 CATCACCATG GTAATAGCGA TGACTAATAC GTAGATGTAC TGCCAAGTAG GAAAGTCCCA
              601 TAAGGTCATG TACTGGGCAT AATGCCAGGC GGGCCATTTA CCGTCATTGA CGTCAATAGG
              661 GGGCGTACTT GGCATATGAT ACACTTGATG TACTGCCAAG TGGGCAGTTT ACCGTAAATA
              721 CTCCACCCAT TGACGTCAAT GGAAAGTCCC TATTGGCGTT ACTATGGGAA CATACGTCAT
              781 TATTGACGTC AATGGGCGGG GGTCGTTGGG CGGTCAGCCA GGCGGGCCAT TTACCGTAAG
            841 TTATGTAACG CGGAACTCCN TATATGGGCT ATGAACTAAT GN
            //
            Primer: cPPT/CTS-E_R
            Region: cPPT_CTS,RRE
            Sequence file: RDB17487_A9I6h.seq check
            >04379_17487_A9I6_3_cPPT_CTS-E_R_D10_10_ABI24.ab1
                1 NNNNNNNNNT NGNTTTTGNA TTCTTTAGTT TGTATGTCTG TTGCTATTAT GTCTACTATT
               61 CTTTCCCCTG CACTGTACCC CCCAATCCCC CCTTTTCTTT TAAAATTGTG GATGAATACT
              121 GCCATTTGTA GATCCGTTCA CTAATCGAAT GGATCTGTCT CTGTCTCTCT CTCCACCTTC
              181 TTCTTCTATT CCTTCGGGCC TGTCGGGTCC CCTCGGGGTT GGGAGGTGGG TCTGAAACGA
              241 TAATGGTGAA TATCCCTGCC TAACTCTATT CACTATAGAA AGTACAGCAA AAACTATTCT
              301 TAAACCTACC AAGCCTCCTA CTATCATTAT GAATAATTTT ATATACCACA GCCAATTTGT
              361 TATGTTAAAC CAATTCCACA AACTTGCCCA TTTATCTAAT TCCAATAATT CTTGTTCATT
              421 CTTTTCTTGC TGGTTTTGCG ATTCTTCAAT TAAGGAGTGT ATTAAGCTTG TGTAATTGTT
              481 AATTTCTCTG TCCCACTCCA TCCAGGTCGT GTGATTCCAA ATCTGTTCCA GAGATTTATT
              541 ACTCCAACTA GCATTCCAAG GCACAGCAGT GGTGCAAATG AGTTTTCCAG AGCAACCCCA
              601 AATCCCCAGG AGCTGTTGAT CCTTTAGGTA TCTTTCCACA GCCAGGATTC TTGCCTGGAG
              661 CTGCTTGATG CCCCAGACTG TGAGTTGCAA CAGATGCTGT TGCGCCTCAA TAGCCCTCAG
              721 CAAATTGTTC TGCTGCTGCA CTATACCAGA CAATAATTGT CTGGCCTGTA CCGTCAGCGT
              781 CATTGACGCT GCGCCCATAG TGCTTCCTGC TGCTCCCNAG AACCCAAGGA ACAAAGCTCC
              841 TATTCCCACT GCTCTTTTTT CTCTCTGCAC CACTCTTCTC TTTGCCNTGG NGGGNGCTAC
            901 TCCNAATGGT TCAATTTTTN ACTACTTNAT ATTTATATAA N
            //
            Primer: BGH_rev2
            Region: bGHp(A),3'LTR
            Sequence file: RDB17487_A9I6i.seq check
            >04379_17487_A9I6_3_BGH_rev2_E10_13_ABI24.ab1
                1 NNNNNNNNNN NNNNNNGGGG GNNGGGNNNA CNACAGATGG CTGGCAACTA GAAGGCACAG
               61 TCGAGGCTGA TCAGCGGGTT TAAACGGGCC CTGCTAGAGA TTTTCCACAC TGACTAAAAG
              121 GGTCTGAGGG ATCTCTAGTT ACCAGAGTCA CACAACAGAC GGGCACACAC TACTTGAAGC
              181 ACTCAAGGCA AGCTTTATTG AGGCTTAAGC AGTGGGTTCC CTAGTTAGCC AGAGAGCTCC
              241 CAGGCTCAGA TCTGGTCTAA CCAGAGAGAC CCAGTACAGT CCGGATGCAG CTCTCGGGCC
              301 ATGTGATGAA ATGCTAGGCG GCTGTCAAAC CTCCACTCTA ATACTTCTCT CTCCGGGTCA
              361 TCCATCCCAT GCAGGCTCAC AGGGTGTAAC AAGCGGGTGT TCTCTCCTTC ATTGGCTTCT
              421 TCTACCTTCT CTTGCTCAAC TGGTACTAGC TTGTAGCACC ATCCAAAGGT CAGTGGATAT
              481 CTGATCCCTG GCCCTGGTGT GTAGTTCTGC CAATCAGGGA AGTAGCCTTG TGTGTGGTAG
              541 ATCCACAGAT CAAGGATATC TTGTCTTCGT TGGGAGTGAA TTAGCCCTTC CAGTCCCCCC
              601 TTTTCTTTTA AAAAGTGGCT AAGATCTACA GCTGCCTTGT AAGTCATTGG TCTTAAAGGT
              661 ACCTGAGGTG TGACTGGAAA ACCCACCTCC TCCTCCTCTT GTGCTTCTAG CCAGGCACAA
              721 TCAGCATTGG TAGCTGCTGT ATTGCTACTT GTGATTGCTC CATGTTTTTC TAGGTCTCGA
            781 CNGTATCGAT GCGGGGANGC GGNCCN
            //
            Primer: SV40polyA_R2
            Region: Zeo,SV40pro_ori
            Sequence file: RDB17487_A9I6j.seq check
            >04379_17487_A9I6_3_SV40polyA_R2_F10_16_ABI24.ab1
                1 NNNNNNNNNC NNNNNNNNNN NGTTGGGNNG GGCGAAGAAC TCCNGCATGA GATCCCCGCG
               61 CTGGAGGANC ATCCAGCCGG CGTCCCGGAA AACGATTCCG AAGCCCAACC TTTCATAGAA
              121 GGCGGCGGTG GAATCGAAAT CTCGTAGCAC GTGTCAGTCC TGCTCCTCGG CCACGAAGTG
              181 CACGCAGTTG CCGGCCGGGT CGCGCAGGGC GAACTCCCGC CCCCACGGCT GCTCGCCGAT
              241 CTCGGTCATG GCCGGCCCGG AGGCGTCCCG GAAGTTCGTG GACACGACCT CCGACCACTC
              301 GGCGTACAGC TCGTCCAGGC CGCGCACCCA CACCCAGGCC AGGGTGTTGT CCGGCACCAC
              361 CTGGTCCTGG ACCGCGCTGA TGAACAGGGT CACGTCGTCC CGGACCACAC CGGCGAAGTC
              421 GTCCTCCACG AAGTCCCGGG AGAACCCGAG CCGGTCGGTC CAGAACTCGA CCGCTCCGGC
              481 GACGTCGCGC GCGGTGAGCA CCGGAACGGC ACTGGTCAAC TTGGCCATGG TTTAGTTCCT
              541 CACCTTGTCG TATTATACTA TGCCGATATA CTATGCCGAT GATTAATTGT CAACACGTGC
              601 TGATCAGATC CGAAAATGGA TATACAAGCT CCCGGGAGCT TTTTGCAAAA GCCTAGGCCT
              661 CCAAAAAAGC CTCCTCACTA CTTCTGGAAT AGCTCAGAGG CAGAGGCGGC CTCGGCCTCT
            721 GCATAAATAA AAAAAATTAG TCAGCCATGG GGCGGANAAT
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Shibuya, K., CD226 (DNAM-1) is involved in lymphocyte function-associated antigen 1 costimulatory signal for naive T cell differentiation and proliferation. J. Exp. Med. 198 (12): 1829-1839 (2003). PMID 14676297.
            original Tahara-Hanaoka, S., Lentiviral vector-mediated transduction of murine CD34(-) hematopoietic stem cells. Exp. Hematol. 30 (1): 11-17 (2002). PMID 11823032.
            original Sumimoto, H., Rapid and efficient generation of lentivirally gene-modified dendritic cells from DC progenitors with bone marrow stromal cells. J. Immunol. Methods 271 (1-2): 153-165 (2002). PMID 12445738.
            original Miyoshi, H., Development of a self-inactivating lentivirus vector. J. Virol., 72 (10): 8150-8157 (1998). PMID 9733856.
            reference Yamaguchi, T., Comparison of gene-trapping efficiency between retroviral and lentiviral vectors in mouse embryonic stem cells. Biochem. Biophys. Res. Commun. 425 (2): 297-303 (2012). PMID 22842569.
            user_report Miyazaki, S., Establishment of a long-term stable beta-cell line and its application to analyze the effect of Gcg expression on insulin secretion. Sci. Rep. 11 (1): 477 (2021). PMID 33436850.
            user_report Murayama, A.Y., The polymicrogyria-associated GPR56 promoter preferentially drives gene expression in developing GABAergic neurons in common marmosets. Sci. Rep. 10 (1): 21516 (2020). PMID 33299078.
            user_report Suzuki, E., Secretion of high amounts of hepatocyte growth factor is a characteristic feature of cancer-associated fibroblasts with EGFR-TKI resistance-promoting phenotype: A study of 18 cases of cancer-associated fibroblasts. Pathol. Int. 69 (8): 472-480 (2019). PMID 31339210.
            user_report Morikawa, M., The ALK-1/SMAD/ATOH8 axis attenuates hypoxic responses and protects against the development of pulmonary arterial hypertension. Sci. Signal. 12(607). pii: eaay4430 (2019). PMID 31719172.
            user_report Ogawa, Y., Elavl3 is essential for the maintenance of Purkinje neuron axons. Sci. Rep. 8 (1): 2722 (2018). PMID 29426875.
            user_report Shamoto, N., CFAP70 Is a Novel Axoneme-Binding Protein That Localizes at the Base of the Outer Dynein Arm and Regulates Ciliary Motility. Cells 7 (9). pii: E124 (2018). PMID 30158508.
            user_report Katsura, A., ZEB1-regulated inflammatory phenotype in breast cancer cells. Mol. Oncol. 11 (9): 1241-1262 (2017). PMID 28618162.
            user_report Kubota, S.I., Whole-Body Profiling of Cancer Metastasis with Single-Cell Resolution. Cell Rep. 20 (1): 236-250 (2017). PMID 28683317.
            user_report Kobayashi, M., Functional Analysis of Novel Candidate Regulators of Insulin Secretion in the MIN6 Mouse Pancreatic beta Cell Line PLoS One 11 (3): e0151927 (2016). PMID 26986842.
            user_report Suzuki, S., Characterization of actions of octanoate on porcine preadipocytes and adipocytes differentiated in vitro. Biochem. Biophys. Res. Commun., 432 (1): 92-98 (2013). PMID 23376076.
            user_report Yaguchi, M., Characterization of the properties of seven promoters in the motor cortex of rats and monkeys after lentiviral vector-mediated gene transfer. Hum. Gene Ther. Methods, 24 (6): 333-344 (2013). PMID 23964981.
            user_report Tamura, K., A glass-coated tungsten microelectrode enclosing optical fibers for optogenetic exploration in primate deep brain structures. J. Neurosci. Methods, 211 (1): 49-57 (2012). PMID 22971353.
            user_report Saitoh, Y., Overexpression of NF-kappaB inducing kinase underlies constitutive NF-kappaB activation in lung cancer cells. Lung. Cancer., 70, 263-270 (2010). PMID 20338663.
            user_report Ohashi Y., A bicistronic lentiviral vector-based method for differential transsynaptic tracing of neural circuits. Mol. Cell. Neurosci. 46 (1): 136-147. PMID 20816792.
            user_report Hanyu, A., Functional in vivo optical imaging of tumor angiogenesis, growth, and metastasis prevented by administration of anti-human VEGF antibody in xenograft model of human fibrosarcoma HT1080 cells. Cancer Sci. 100 (11): 2085-2092 (2009). PMID 19719773.
            user_report Yoshida, S., Knockdown of synapse-associated protein Dlg1 reduces syncytium formation induced by human T-cell leukemia virus type 1. Virus Genes, 37 (1): 9-15 (2008). PMID 18461433.
            user_report Inoue-Narita, T., Pten deficiency in melanocytes results in resistance to hair graying and susceptibility to carcinogen-induced melanomagenesis. Cancer Res. 2008 Jul 15;68(14):5760-8 (2008). PMID 18632629.
            user_report Ehata, S., Ki26894, a novel transforming growth factor-beta type I receptor kinase inhibitor, inhibits in vitro invasion and in vivo bone metastasis of a human breast cancer cell line. Cancer Sci. 98 (1): 127-133 (2007). PMID 17129361.
            user_report Lu F.Z., Long-term gene expression using the lentiviral vector in rat chondrocytes. Clin. Orthop. Relat. Res., 439, 243-252 (2005). PMID 16205166.
            user_report Lu F.Z., Characterization and gene transfer in mesenchymal stem cells derived from human umbilical-cord blood. J. Lab. Clin. Med., 146, 271-278 (2005). PMID 16242526.

            2022.05.18

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