Resource data sheet
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CS-CDF-CG-PRE

Shuttle vector to generate recombinant Lentiviral vector expressing GFP

Catalog number RDB04379
Resource name CS-CDF-CG-PRE
Clone info. Shuttle vector to generate recombinant Lentiviral vector expressing GFP.
Commonly requested with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394).
Vector backbone Plasmid
Selectable markers Amp^r
Originator Miyoshi, Hiroyuki |

Distribution information

CLICK here for plasmid list and map. [link]
Forms for plasmid request [in Japanese] [in English]
Terms and conditions for distribution The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose.
(Japanese text [open/close])

Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB13006_A4K5p1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CSII-CMV5' RDB13006_A4K5a.seq
>04379_13006_A4K5_CSII-CMV5'_B09_06.ab1
    1 CACGGCACCG CTCGGTCGCC ACCATGGTGA GCAAGGGCGA GGAGCTGTTC ACCGGGGTGG
   61 TGCCCATCCT GGTCGAGCTG GACGGCGACG TAAACGGCCA CAAGTTCAGC GTGTCCGGCG
  121 AGGGCGAGGG CGATGCCACC TACGGCAAGC TGACCCTGAA GTTCATCTGC ACCACCGGCA
  181 AGCTGCCCGT GCCCTGGCCC ACCCTCGTGA CCACCCTGAC CTACGGCGTG CAGTGCTTCA
  241 GCCGCTACCC CGACCACATG AAGCAGCACG ACTTCTTCAA GTCCGCCATG CCCGAAGGCT
  301 ACGTCCAGGA GCGCACCATC TTCTTCAAGG ACGACGGCAA CTACAAGACC CGCGCCGAGG
  361 TGAAGTTCGA GGGCGACACC CTGGTGAACC GCATCGAGCT GAAGGGCATC GACTTCAAGG
  421 AGGACGGCAA CATCCTGGGG CACAAGCTGG AGTACAACTA CAACAGCCAC AACGTCTATA
  481 TCATGGCCGA CAAGCAGAAG AACGGCATCA AGGTGAACTT CAAGATCCGC CACAACATCG
  541 AGGACGGCAG CGTGCAGCTC GCCGACCACT ACCAGCAGAA CACCCCCATC GGCGACGGCC
  601 CCGTGCTGCT GCCCGACAAC CACTACCTGA GCACCCAGTC CGCCCTGAGC AAAGACCCCA
  661 ACGAGAAGCG CGATCACATG GTCCTGCTGG AGTTCGTGAC CGCCGCCGGG ATCACTCTCG
  721 GCATGGACGA GCTGTACAAG TCCGGACTCA GATCCTACTA GTAGGATCTC GAGGGATCAA
  781 GCTTATCGAT AATCAACCTC TGGATTACAA AATTTGTGAA AGATTGACTG GTATTCTTAA
  841 CTATGTTGCT CCTTTTACGC TATGTGGATA CGCTGCTTTA ATGCCTTTGT ATCATGCTAT
  901 TGCTTCCCGT ATGGCTTTCA TTTTCTCCTC CTTGTATAAA TCCTGGTTGC TGTCTCTTTA
  961 TGAGGAGTTG TGGCCCGTTG TCAGGCAACG TGGCGTGGTG TGCACTGTGT TTGCTGACGC
 1021 AACCCCCACT GGTTTGGGGG CATTGCCACC CACCTGTCAG CTCCTTTCCG GACTTTCGCT
 1081 TTCCCCCTTC CTATGGCACG CCGACTCATC GACGCCTGCC GTTGGCCCCG CCTGCCTGGG
//
Primer: BGH_rev RDB13006_A4K5b.seq
>04379_13006_A4K5_BGH_rev_C09_09.ab1
    1 GTTTTCGGGG GTCTGCTAGA GATTTTCCAC ACTGACTAAA AGGGTCTGAG GGATCTCTAG
   61 TTACCAGAGT CACACAACAG ACGGGCACAC ACTACTTGAA GCACTCAAGG CAAGCTTTAT
  121 TGAGGCTTAA GCAGTGGGTT CCCTAGTTAG CCAGAGAGCT CCCAGGCTCA GATCTGGTCT
  181 AACCAGAGAG ACCCAGTACA GTCCGGATGC AGCTCTCGGG CCATGTGATG AAATGCTAGG
  241 CGGCTGTCAA ACCTCCACTC TAATACTTCT CTCTCCGGGT CATCCATCCC ATGCAGGCTC
  301 ACAGGGTGTA ACAAGCGGGT GTTCTCTCCT TCATTGGCTT CTTCTACCTT CTCTTGCTCA
  361 ACTGGTACTA GCTTGTAGCA CCATCCAAAG GTCAGTGGAT ATCTGATCCC TGGCCCTGGT
  421 GTGTAGTTCT GCCAATCAGG GAAGTAGCCT TGTGTGTGGT AGATCCACAG ATCAAGGATA
  481 TCTTGTCTTC GTTGGGAGTG AATTAGCCCT TCCAGTCCCC CCTTTTCTTT TAAAAAGTGG
  541 CTAAGATCTA CAGCTGCCTT GTAAGTCATT GGTCTTAAAG GTACCTGAGG TGTGACTGGA
  601 AAACCCACCT CCTCCTCCTC TTGTGCTTCT AGCCAGGCAC AATCAGCATT GGTAGCTGCT
  661 GTATTGCTAC TTGTGATTGC TCCATGTTTT TCTAGGTCTC GACGGTATCG ATGCGGGGAG
  721 GCGGCCCAAA GGGAGATCCG ACTCGTCTGA GGGCGAAGGC GAAGACGCGG AAGAGGCCGC
  781 AGAGCCGGCA GCAGGCCGCG GGAAGGAAGG TCCGCTGGAT TGAGGGCCGA AGGGACGTAG
  841 CAGAAGGACG TCCCGCGCAG AATCCAGGTG GCAACACAGG CGAGCAGCCA TGGAAAGGAC
  901 GTCAGCTTCC CCGACAACAC CACGGAATTG TCAGTGCCCA ACAGCCGAGC CCCTGTCCAG
  961 CAGCGGGCAA GGCAGGCGGC GATGAGTTCC GCCGTGGCAA TAGGGAGGGG GAAAGCGAAA
 1021 GTCCCGGGAA AGGGAGCTGA CAGGTGGTGG CAATGCCCCA ACCAGTGGGG GGTTGCGTCA
 1081 GCAACACAGT GCACACCACG CCACGTTTGC CCTGACACCG GACACACTTC TTCATAAAGA
1141 AGACAGCCAG CCAGTATTTT AATACCAACG GAAGGGCA
//
Primer: HIVgp_R1 RDB13006_A4K5c.seq
>04379_13006_A4K5_HIVgp_R1_D09_12.ab1
    1 CAGCTTCTAT TCCACTGCTC TTTTTTCTCT CTGCACCACT CTTCTCTTTG CCTTGGTGGG
   61 TGCTACTCCT AATGGTTCAA TTTTTACTAC TTTATATTTA TATAATTCAC TTCTCCAATT
  121 GTCCCTCATA TCTCCTCCTC CAGGTCTGAA GATCAGCGGC CGCTTGCTGT GCGGTGGTCT
  181 TACTTTTGTT TTGCTCTTCC TCTATCTTGT CTAAAGCTTC CTTGGTGTCT TTTATCTCTA
  241 TCCTTTGATG CACACAATAG AGGGTTGCTA CTGTATTATA TAATGATCTA AGTTCTTCTG
  301 ATCCTGTCTG AAGGGATGGT TGTAGCTGTC CCAGTATTTG TCTACAGCCT TCTGATGTTT
  361 CTAACAGGCC AGGATTAACT GCGAATCGTT CTAGCTCCCT GCTTGCCCAT ACTATATGTT
  421 TTAATTTATA TTTTTTCTTT CCCCCTGGCC TTAACCGAAT TTTTTCCCAT CGCGATCTAA
  481 TTCTCCCCCG CTTAATACTG ACGCTCTCGC ACCCATCTCT CTCCTTCTAG CCTCCGCTAG
  541 TCAAAATTTT TGGCGTACTC ACCAGTCGCC GCCCCTCGCC TCTTGCCGTG CGCGCTTCAG
  601 CAAGCCGAGT CCTGCGTCGA GAGAGCTCCT CTGGTTTCCC TTTCGCTTTC AAGTCCCTGT
  661 TCGGGCGCCA CTGCTAGAGA TTTTCCACAC TGACTAAAAG GGTCTGAGGG ATCTCTAGTT
  721 ACCAGAGTCA CACAACAGAC GGGCACACAC TACTTGAAGC ACTCAAGGCA AGCTTTATTG
  781 AGGCTTAAGC AGTGGGTTCC CTAGTTAGCC AGAGAGCTCC CAGGCTCAGA TCTGGTCTAA
  841 CCAGAGAGAC CCAGTACAGG CAAAACGCGC TGCTTATATA GACCTCCCAC CGTACACGCC
  901 TACCGCCCAT TTGCGTCAAT GGGGCGGAGT TGTTACGACA TTTTGGAAAG TCCCGTTGAT
  961 TTTGGTGCCA AAACAAACTC CCATTGACGT CAATGGGGTG GAGACTTGGA AATCCCCGTG
 1021 AGTCAAACCC GCTATCCACG CCCATTGATG TACTGCCAAA ACCGCATCAC CATGGGTAAT
 1081 AGGCGGATGA CTAATACGTA GATGTACTTA CTAGGTTAGA AGGTCCCATA GGTCATGTAC
1141 TGGGGCATAA ATTGACCAGG GGCGGGGACC AT
//
Primer: SV40pro_F RDB13006_A4K5d.seq
>04379_13006_A4K5_SV40pro_F_E09_15.ab1
    1 CCTTGTCTTT GACATTCAGA AGTAGTGAGG AGGCTTTTTT GGAGGCCTAG GCTTTTGCAA
   61 AAAGCTCCCG GGAGCTTGTA TATCCATTTT CGGATCTGAT CAGCACGTGT TGACAATTAA
  121 TCATCGGCAT AGTATATCGG CATAGTATAA TACGACAAGG TGAGGAACTA AACCATGGCC
  181 AAGTTGACCA GTGCCGTTCC GGTGCTCACC GCGCGCGACG TCGCCGGAGC GGTCGAGTTC
  241 TGGACCGACC GGCTCGGGTT CTCCCGGGAC TTCGTGGAGG ACGACTTCGC CGGTGTGGTC
  301 CGGGACGACG TGACCCTGTT CATCAGCGCG GTCCAGGACC AGGTGGTGCC GGACAACACC
  361 CTGGCCTGGG TGTGGGTGCG CGGCCTGGAC GAGCTGTACG CCGAGTGGTC GGAGGTCGTG
  421 TCCACGAACT TCCGGGACGC CTCCGGGCCG GCCATGACCG AGATCGGCGA GCAGCCGTGG
  481 GGGCGGGAGT TCGCCCTGCG CGACCCGGCC GGCAACTGCG TGCACTTCGT GGCCGAGGAG
  541 CAGGACTGAC ACGTGCTACG AGATTTCGAT TCCACCGCCG CCTTCTATGA AAGGTTGGGC
  601 TTCGGAATCG TTTTCCGGGA CGCCGGCTGG ATGATCCTCC AGCGCGGGGA TCTCATGCTG
  661 GAGTTCTTCG CCCACCCCAA CTTGTTTATT GCAGCTTATA ATGGTTACAA ATAAAGCAAT
  721 AGCATCACAA ATTTCACAAA TAAAGCATTT TTTTCACTGC ATTCTAGTTG TGGTTTGTCC
  781 AAACTCATCA ATGTATCTTA TCATGTCTGT ATACCGTCGA CCTCTAGCTA GAGCTTGGCG
  841 TAATCATGGT CATAGCTGTT TCCTGTGTGA AATTGTTATC CGCTCACAAT TCCACACAAC
  901 ATACGAGCCG GAAGCATAAA GTGTAAAGCC TGGGGTGCCT AATGAGTGAG CTAACTCACA
  961 TTAATTGCGT TGCGCTCACT GCCCGCTTTC CAGTCGGGAA ACCTGTCGTG CCAGCTGCAT
 1021 TAATGAATCG GCCAACGCGC GGGGCAGAGC GGGTTTGCGT ATTGGCCGCT CTTCCGCTTC
 1081 TCGCTCACTG ACTCGCTGCG CTTCGGTCGC TCCGGCCTGC GGCGAGCCGT AATCCAGCTC
1141 CACCTCAGTC GTATACGCTA TTCCCAACAA
//

Please visit plasmidSequencing and PCR primers for primer information.

References and tips

Electronic file

References

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user report Su, S., Genetically encoded calcium indicator illuminates calcium dynamics in primary cilia. Nat. Methods, 10 (11): 1105-1107 (2013). PMID 24056873.
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2017.10.23

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