RDB04379 - CS-CDF-CG-PRE

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CS-CDF-CG-PRE

Lentivirus vector plasmid of GFP driven by CMV promoter.

Catalog number	RDB04379
Resource name	CS-CDF-CG-PRE
Clone info.	Lentivirus vector plasmid of GFP driven by CMV promoter.
Comment	Commonly requested with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Vector backbone	Plasmid
Selectable markers	Please note that Zeo resistance marker is not included in the lentivirus produced from this vector. E. coli: Amp^r.
Depositor\|Developer	Miyoshi, Hiroyuki \|

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External Database

Reference sequence

Lentiviral plasmid list. [link]

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer.

Ordering Please visit Ordering instruction.[link]
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Material Transfer Agreement (MTA, for use for not-for-profit academic purpose)[Word]
This RESOURCE contains a fluorescent protein owned or licensed by Global Life Sciences Solutions Operations UK Ltd. (Cytiva). Please refer to Schedule B and use Schedule A (download the Schedule A and Schedule B [link]).

Remarks ((Remarks)) Remember that you will be working with samples containing infectious virus.

提供案内 (日本国内) [open/close]

Terms and conditions set forth by the DEPOSITOR	The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer.
Ordering	Please visit Ordering instruction.[link] Order form [Credit Card Payment] [Bank Transfer Payment] Material Transfer Agreement (MTA, for use for not-for-profit academic purpose)[Word] This RESOURCE contains a fluorescent protein owned or licensed by Global Life Sciences Solutions Operations UK Ltd. (Cytiva). Please refer to Schedule B and use Schedule A (download the Schedule A and Schedule B [link]).
Remarks	((Remarks)) Remember that you will be working with samples containing infectious virus.

提供条件	本件研究材料は、非営利機関の非営利学術研究に限って提供する。本件研究材料を利用した研究結果等を発表する際は、本件研究材料が故三好浩之博士により開発されたことを明示する。
提供依頼	手続きの詳細は、「レンチウイルスベクターの提供申し込み」をご覧ください。提供依頼書 [Word] 提供同意書 (非営利機関による非営利学術研究用)[Word] GFPリソースを含みます。Schedule Bをご一読の上Schedule A (英語版のみ) の提出をお願いします（Schedule AとSchedule Bを入手[link])。遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。
備考	【使用上の注意】このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB04379	CS-CDF-CG-PRE	DNA solution

Ordering instruction of plasmids [in Japanese] [in English]
Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

References and tips

Electronic file

Sequence	RDB04379z.seq
Remarks, protocol and/or map (pdf)	RDB04379.pdf

Original reference

original	Shibuya, K., CD226 (DNAM-1) is involved in lymphocyte function-associated antigen 1 costimulatory signal for naive T cell differentiation and proliferation. J. Exp. Med. 198 (12): 1829-1839 (2003). PMID 14676297.
original	Tahara-Hanaoka, S., Lentiviral vector-mediated transduction of murine CD34(-) hematopoietic stem cells. Exp. Hematol. 30 (1): 11-17 (2002). PMID 11823032.
original	Sumimoto, H., Rapid and efficient generation of lentivirally gene-modified dendritic cells from DC progenitors with bone marrow stromal cells. J. Immunol. Methods 271 (1-2): 153-165 (2002). PMID 12445738.
original	Miyoshi, H., Development of a self-inactivating lentivirus vector. J. Virol., 72 (10): 8150-8157 (1998). PMID 9733856.

Further references such as user reports and related articles (go to bottom)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB17487_A9I6p1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: EGFP-M_R Region: EGFP,CMV pro Sequence file: RDB17487_A9I6e.seq
>04379_17487_A9I6_3_EGFP-M_R_A01_01_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNGGNNCTT GNNNTTNNCC TTGNTGCCGT TCTTCTGCTT 61 GTCGGCCATG ATATANNNNT TGTGGCTGTT GTAGTTGTAC TCCAGCTTGT GCCCCAGGAT 121 GTTGCCGTCC TCCTTGAAGT CGATGCCCTT CAGCTCGATG CGGTTCACCA GGGTGTCGCC 181 CTCGAACTTC ACCTCGGCGC GGGTCTTGTA GTTGCCGTCG TCCTTGAAGA AGATGGTGCG 241 CTCCTGGACG TAGCCTTCGG GCATGGCGGA CTTGAAGAAG TCGTGCTGCT TCATGTGGTC 301 GGGGTAGCGG CTGAAGCACT GCACGCCGTA GGTCAGGGTG GTCACGAGGG TGGGCCAGGG 361 CACGGGCAGC TTGCCGGTGG TGCAGATGAA CTTCAGGGTC AGCTTGCCGT AGGTGGCATC 421 GCCCTCGCCC TCGCCGGACA CGCTGAACTT GTGGCCGTTT ACGTCGCCGT CCAGCTCGAC 481 CAGGATGGGC ACCACCCCGG TGAACAGCTC CTCGCCCTTG CTCACCATGG TGGCGACCGG 541 TAGCGCTAGC GGATCTGACG GTTCACTAAA CCAGCTCTGC TTATATAGAC CTCCCACCGT 601 ACACGCCTAC CGCCCATTTG CGTCAATGGG GCGGAGTTGT TACGACATTT TGGAAAGTCC 661 CGTTGATTTT GGTGCCAAAA CAAACTCCCA TTGACGTCAA TGGGGTGGAG ACTTGGAAAT 721 CCCCGTGAGT CAAACCGCTA TCCACGCCCA TTGATGTACT GCCAAAACCG CATCANCATG 781 GTAATAGCGA TGACTAATAC GTANATGTAC TGCCAAGTAN GAAAGTCCCA TAANGTCATG 841 TACTGGGCAT AATGCNAGGC GGGCCATTTA NCGTCNTTGA CGTCNATAGG GGGCGTACTT 901 GGNCATATGA TACNCTTGAT GTACTGCC //
Primer: PRE_rev Region: PRE,EGFP Sequence file: RDB17487_A9I6f.seq
>04379_17487_A9I6_3_PRE_rev_B10_04_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNANCNNN ATAGTTAAGA ATACCAGTCA ATCTTTCNCA 61 AATTTTGTAA TCCAGAGGTT GATTATCGAT AAGCTTGATC CCTCGAGATC CTACTAGTAG 121 GATCTGAGTC CGGACTTGTA CAGCTCGTCC ATGCCGAGAG TGATCCCGGC GGCGGTCACG 181 AACTCCAGCA GGACCATGTG ATCGCGCTTC TCGTTGGGGT CTTTGCTCAG GGCGGACTGG 241 GTGCTCAGGT AGTGGTTGTC GGGCAGCAGC ACGGGGCCGT CGCCGATGGG GGTGTTCTGC 301 TGGTAGTGGT CGGCGAGCTG CACGCTGCCG TCCTCGATGT TGTGGCGGAT CTTGAAGTTC 361 ACCTTGATGC CGTTCTTCTG CTTGTCGGCC ATGATATAGA CGTTGTGGCT GTTGTAGTTG 421 TACTCCAGCT TGTGCCCCAG GATGTTGCCG TCCTCCTTGA AGTCGATGCC CTTCAGCTCG 481 ATGCGGTTCA CCAGGGTGTC GCCCTCGAAC TTCACCTCGG CGCGGGTCTT GTAGTTGCCG 541 TCGTCCTTGA AGAAGATGGT GCGCTCCTGG ACGTAGCCTT CGGGCATGGC GGACTTGAAG 601 AAGTCGTGCT GCTTCATGTG GTCGGGGTAG CGGCTGAAGC ACTGCACGCC GTAGGTCANG 661 GTGGTCACGA GGGTGGGCCA GGGCACGGGC AGCTTGCCGG TGGTGCAGAT GAACTTCANG 721 GTCAGCTTGC CGTANGTGGC ATCGCCCTCG CCCTCGCCGG ACACGCTGAA CTTGTGGCCG 781 TTTACGTCGC CGTCCAGCTC GACCAGGATG GGCACCACCC CGGTGAACAG CTCCTCGCCC 841 TTGCTCACCA TGGTGGCGAC CGGTAGCGCT AGCGGATCTG ACGGTTCACT NANN //
Primer: HIV-B_R Region: HIV-1psi,5'LTR,CMVpro Sequence file: RDB17487_A9I6g.seq
>04379_17487_A9I6_3_HIV-B_R_C10_07_ABI24.ab1 1 NNNNNNNNNN NNNNCNNNNN NNNNTTTTGG NGTACTCNCC NGTCGCCGCC CCTCGCCTCT 61 TGCCGTGCGC GCTTCAGCAA GCCGAGTCCT GCGTCGAGAG AGCTCCTCTG GTTTCCCTTT 121 CGCTTTCAAG TCCCTGTTCG GGCGCCACTG CTAGAGATTT TCCACACTGA CTAAAAGGGT 181 CTGAGGGATC TCTAGTTACC AGAGTCACAC AACAGACGGG CACACACTAC TTGAAGCACT 241 CAAGGCAAGC TTTATTGAGG CTTAAGCAGT GGGTTCCCTA GTTAGCCAGA GAGCTCCCAG 301 GCTCAGATCT GGTCTAACCA GAGAGACCCA GTACAGGCAA AACGCGCTGC TTATATAGAC 361 CTCCCACCGT ACACGCCTAC CGCCCATTTG CGTCAATGGG GCGGAGTTGT TACGACATTT 421 TGGAAAGTCC CGTTGATTTT GGTGCCAAAA CAAACTCCCA TTGACGTCAA TGGGGTGGAG 481 ACTTGGAAAT CCCCGTGAGT CAAACCGCTA TCCACGCCCA TTGATGTACT GCCAAAACCG 541 CATCACCATG GTAATAGCGA TGACTAATAC GTAGATGTAC TGCCAAGTAG GAAAGTCCCA 601 TAAGGTCATG TACTGGGCAT AATGCCAGGC GGGCCATTTA CCGTCATTGA CGTCAATAGG 661 GGGCGTACTT GGCATATGAT ACACTTGATG TACTGCCAAG TGGGCAGTTT ACCGTAAATA 721 CTCCACCCAT TGACGTCAAT GGAAAGTCCC TATTGGCGTT ACTATGGGAA CATACGTCAT 781 TATTGACGTC AATGGGCGGG GGTCGTTGGG CGGTCAGCCA GGCGGGCCAT TTACCGTAAG 841 TTATGTAACG CGGAACTCCN TATATGGGCT ATGAACTAAT GN //
Primer: cPPT/CTS-E_R Region: cPPT_CTS,RRE Sequence file: RDB17487_A9I6h.seq
>04379_17487_A9I6_3_cPPT_CTS-E_R_D10_10_ABI24.ab1 1 NNNNNNNNNT NGNTTTTGNA TTCTTTAGTT TGTATGTCTG TTGCTATTAT GTCTACTATT 61 CTTTCCCCTG CACTGTACCC CCCAATCCCC CCTTTTCTTT TAAAATTGTG GATGAATACT 121 GCCATTTGTA GATCCGTTCA CTAATCGAAT GGATCTGTCT CTGTCTCTCT CTCCACCTTC 181 TTCTTCTATT CCTTCGGGCC TGTCGGGTCC CCTCGGGGTT GGGAGGTGGG TCTGAAACGA 241 TAATGGTGAA TATCCCTGCC TAACTCTATT CACTATAGAA AGTACAGCAA AAACTATTCT 301 TAAACCTACC AAGCCTCCTA CTATCATTAT GAATAATTTT ATATACCACA GCCAATTTGT 361 TATGTTAAAC CAATTCCACA AACTTGCCCA TTTATCTAAT TCCAATAATT CTTGTTCATT 421 CTTTTCTTGC TGGTTTTGCG ATTCTTCAAT TAAGGAGTGT ATTAAGCTTG TGTAATTGTT 481 AATTTCTCTG TCCCACTCCA TCCAGGTCGT GTGATTCCAA ATCTGTTCCA GAGATTTATT 541 ACTCCAACTA GCATTCCAAG GCACAGCAGT GGTGCAAATG AGTTTTCCAG AGCAACCCCA 601 AATCCCCAGG AGCTGTTGAT CCTTTAGGTA TCTTTCCACA GCCAGGATTC TTGCCTGGAG 661 CTGCTTGATG CCCCAGACTG TGAGTTGCAA CAGATGCTGT TGCGCCTCAA TAGCCCTCAG 721 CAAATTGTTC TGCTGCTGCA CTATACCAGA CAATAATTGT CTGGCCTGTA CCGTCAGCGT 781 CATTGACGCT GCGCCCATAG TGCTTCCTGC TGCTCCCNAG AACCCAAGGA ACAAAGCTCC 841 TATTCCCACT GCTCTTTTTT CTCTCTGCAC CACTCTTCTC TTTGCCNTGG NGGGNGCTAC 901 TCCNAATGGT TCAATTTTTN ACTACTTNAT ATTTATATAA N //
Primer: BGH_rev2 Region: bGHp(A),3'LTR Sequence file: RDB17487_A9I6i.seq
>04379_17487_A9I6_3_BGH_rev2_E10_13_ABI24.ab1 1 NNNNNNNNNN NNNNNNGGGG GNNGGGNNNA CNACAGATGG CTGGCAACTA GAAGGCACAG 61 TCGAGGCTGA TCAGCGGGTT TAAACGGGCC CTGCTAGAGA TTTTCCACAC TGACTAAAAG 121 GGTCTGAGGG ATCTCTAGTT ACCAGAGTCA CACAACAGAC GGGCACACAC TACTTGAAGC 181 ACTCAAGGCA AGCTTTATTG AGGCTTAAGC AGTGGGTTCC CTAGTTAGCC AGAGAGCTCC 241 CAGGCTCAGA TCTGGTCTAA CCAGAGAGAC CCAGTACAGT CCGGATGCAG CTCTCGGGCC 301 ATGTGATGAA ATGCTAGGCG GCTGTCAAAC CTCCACTCTA ATACTTCTCT CTCCGGGTCA 361 TCCATCCCAT GCAGGCTCAC AGGGTGTAAC AAGCGGGTGT TCTCTCCTTC ATTGGCTTCT 421 TCTACCTTCT CTTGCTCAAC TGGTACTAGC TTGTAGCACC ATCCAAAGGT CAGTGGATAT 481 CTGATCCCTG GCCCTGGTGT GTAGTTCTGC CAATCAGGGA AGTAGCCTTG TGTGTGGTAG 541 ATCCACAGAT CAAGGATATC TTGTCTTCGT TGGGAGTGAA TTAGCCCTTC CAGTCCCCCC 601 TTTTCTTTTA AAAAGTGGCT AAGATCTACA GCTGCCTTGT AAGTCATTGG TCTTAAAGGT 661 ACCTGAGGTG TGACTGGAAA ACCCACCTCC TCCTCCTCTT GTGCTTCTAG CCAGGCACAA 721 TCAGCATTGG TAGCTGCTGT ATTGCTACTT GTGATTGCTC CATGTTTTTC TAGGTCTCGA 781 CNGTATCGAT GCGGGGANGC GGNCCN //
Primer: SV40polyA_R2 Region: Zeo,SV40pro_ori Sequence file: RDB17487_A9I6j.seq
>04379_17487_A9I6_3_SV40polyA_R2_F10_16_ABI24.ab1 1 NNNNNNNNNC NNNNNNNNNN NGTTGGGNNG GGCGAAGAAC TCCNGCATGA GATCCCCGCG 61 CTGGAGGANC ATCCAGCCGG CGTCCCGGAA AACGATTCCG AAGCCCAACC TTTCATAGAA 121 GGCGGCGGTG GAATCGAAAT CTCGTAGCAC GTGTCAGTCC TGCTCCTCGG CCACGAAGTG 181 CACGCAGTTG CCGGCCGGGT CGCGCAGGGC GAACTCCCGC CCCCACGGCT GCTCGCCGAT 241 CTCGGTCATG GCCGGCCCGG AGGCGTCCCG GAAGTTCGTG GACACGACCT CCGACCACTC 301 GGCGTACAGC TCGTCCAGGC CGCGCACCCA CACCCAGGCC AGGGTGTTGT CCGGCACCAC 361 CTGGTCCTGG ACCGCGCTGA TGAACAGGGT CACGTCGTCC CGGACCACAC CGGCGAAGTC 421 GTCCTCCACG AAGTCCCGGG AGAACCCGAG CCGGTCGGTC CAGAACTCGA CCGCTCCGGC 481 GACGTCGCGC GCGGTGAGCA CCGGAACGGC ACTGGTCAAC TTGGCCATGG TTTAGTTCCT 541 CACCTTGTCG TATTATACTA TGCCGATATA CTATGCCGAT GATTAATTGT CAACACGTGC 601 TGATCAGATC CGAAAATGGA TATACAAGCT CCCGGGAGCT TTTTGCAAAA GCCTAGGCCT 661 CCAAAAAAGC CTCCTCACTA CTTCTGGAAT AGCTCAGAGG CAGAGGCGGC CTCGGCCTCT 721 GCATAAATAA AAAAAATTAG TCAGCCATGG GGCGGANAAT //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original	Shibuya, K., CD226 (DNAM-1) is involved in lymphocyte function-associated antigen 1 costimulatory signal for naive T cell differentiation and proliferation. J. Exp. Med. 198 (12): 1829-1839 (2003). PMID 14676297.
original	Tahara-Hanaoka, S., Lentiviral vector-mediated transduction of murine CD34(-) hematopoietic stem cells. Exp. Hematol. 30 (1): 11-17 (2002). PMID 11823032.
original	Sumimoto, H., Rapid and efficient generation of lentivirally gene-modified dendritic cells from DC progenitors with bone marrow stromal cells. J. Immunol. Methods 271 (1-2): 153-165 (2002). PMID 12445738.
original	Miyoshi, H., Development of a self-inactivating lentivirus vector. J. Virol., 72 (10): 8150-8157 (1998). PMID 9733856.
reference	Yamaguchi, T., Comparison of gene-trapping efficiency between retroviral and lentiviral vectors in mouse embryonic stem cells. Biochem. Biophys. Res. Commun. 425 (2): 297-303 (2012). PMID 22842569.
user_report	Miyazaki, S., Establishment of a long-term stable beta-cell line and its application to analyze the effect of Gcg expression on insulin secretion. Sci. Rep. 11 (1): 477 (2021). PMID 33436850.
user_report	Murayama, A.Y., The polymicrogyria-associated GPR56 promoter preferentially drives gene expression in developing GABAergic neurons in common marmosets. Sci. Rep. 10 (1): 21516 (2020). PMID 33299078.
user_report	Suzuki, E., Secretion of high amounts of hepatocyte growth factor is a characteristic feature of cancer-associated fibroblasts with EGFR-TKI resistance-promoting phenotype: A study of 18 cases of cancer-associated fibroblasts. Pathol. Int. 69 (8): 472-480 (2019). PMID 31339210.
user_report	Morikawa, M., The ALK-1/SMAD/ATOH8 axis attenuates hypoxic responses and protects against the development of pulmonary arterial hypertension. Sci. Signal. 12(607). pii: eaay4430 (2019). PMID 31719172.
user_report	Ogawa, Y., Elavl3 is essential for the maintenance of Purkinje neuron axons. Sci. Rep. 8 (1): 2722 (2018). PMID 29426875.
user_report	Shamoto, N., CFAP70 Is a Novel Axoneme-Binding Protein That Localizes at the Base of the Outer Dynein Arm and Regulates Ciliary Motility. Cells 7 (9). pii: E124 (2018). PMID 30158508.
user_report	Katsura, A., ZEB1-regulated inflammatory phenotype in breast cancer cells. Mol. Oncol. 11 (9): 1241-1262 (2017). PMID 28618162.
user_report	Kubota, S.I., Whole-Body Profiling of Cancer Metastasis with Single-Cell Resolution. Cell Rep. 20 (1): 236-250 (2017). PMID 28683317.
user_report	Kobayashi, M., Functional Analysis of Novel Candidate Regulators of Insulin Secretion in the MIN6 Mouse Pancreatic beta Cell Line PLoS One 11 (3): e0151927 (2016). PMID 26986842.
user_report	Suzuki, S., Characterization of actions of octanoate on porcine preadipocytes and adipocytes differentiated in vitro. Biochem. Biophys. Res. Commun., 432 (1): 92-98 (2013). PMID 23376076.
user_report	Yaguchi, M., Characterization of the properties of seven promoters in the motor cortex of rats and monkeys after lentiviral vector-mediated gene transfer. Hum. Gene Ther. Methods, 24 (6): 333-344 (2013). PMID 23964981.
user_report	Tamura, K., A glass-coated tungsten microelectrode enclosing optical fibers for optogenetic exploration in primate deep brain structures. J. Neurosci. Methods, 211 (1): 49-57 (2012). PMID 22971353.
user_report	Saitoh, Y., Overexpression of NF-kappaB inducing kinase underlies constitutive NF-kappaB activation in lung cancer cells. Lung. Cancer., 70, 263-270 (2010). PMID 20338663.
user_report	Ohashi Y., A bicistronic lentiviral vector-based method for differential transsynaptic tracing of neural circuits. Mol. Cell. Neurosci. 46 (1): 136-147. PMID 20816792.
user_report	Hanyu, A., Functional in vivo optical imaging of tumor angiogenesis, growth, and metastasis prevented by administration of anti-human VEGF antibody in xenograft model of human fibrosarcoma HT1080 cells. Cancer Sci. 100 (11): 2085-2092 (2009). PMID 19719773.
user_report	Yoshida, S., Knockdown of synapse-associated protein Dlg1 reduces syncytium formation induced by human T-cell leukemia virus type 1. Virus Genes, 37 (1): 9-15 (2008). PMID 18461433.
user_report	Inoue-Narita, T., Pten deficiency in melanocytes results in resistance to hair graying and susceptibility to carcinogen-induced melanomagenesis. Cancer Res. 2008 Jul 15;68(14):5760-8 (2008). PMID 18632629.
user_report	Ehata, S., Ki26894, a novel transforming growth factor-beta type I receptor kinase inhibitor, inhibits in vitro invasion and in vivo bone metastasis of a human breast cancer cell line. Cancer Sci. 98 (1): 127-133 (2007). PMID 17129361.
user_report	Lu F.Z., Long-term gene expression using the lentiviral vector in rat chondrocytes. Clin. Orthop. Relat. Res., 439, 243-252 (2005). PMID 16205166.
user_report	Lu F.Z., Characterization and gene transfer in mesenchymal stem cells derived from human umbilical-cord blood. J. Lab. Clin. Med., 146, 271-278 (2005). PMID 16242526.

2022.07.13

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