Resource data sheet
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CS-CDF-CG-PRE

Lentivirus vector construct of GFP driven by CMV promoter.

Catalog number RDB04379
Resource name CS-CDF-CG-PRE
Clone info. Lentivirus vector construct of GFP driven by CMV promoter.
Commonly requested with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Vector backbone Plasmid
Selectable markers Please note that Zeo resistance marker is not included in the lentivirus produced from this vector. E. coli: Amp^r.
Originator Miyoshi, Hiroyuki |
Lentiviral plasmid list, maps and sequences information. [link]

Distribution information

Terms and conditions for distribution The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe Dr. Hiroyuki Miyoshi as the Developer.
Ordering Please visit Ordering instruction. 
Order form 
[Credit Card Payment]  [Bank Transfer or Check Payment]
Material Transfer Agreement (MTA) [Word]
Remarks ((Remarks)) Remember that you will be working with samples containing infectious virus.
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB04379 CS-CDF-CG-PRE DNA solution

Ordering instruction of plasmids [in Japanese] [in English]

References and tips

Electronic file

Electronic file Sequence RDB04379z.seq from Depositor
Electronic file Remarks, protocol and/or map (pdf) RDB04379.pdf from Depositor

Original reference

original Shibuya, K., CD226 (DNAM-1) is involved in lymphocyte function-associated antigen 1 costimulatory signal for naive T cell differentiation and proliferation. J. Exp. Med. 198 (12): 1829-1839 (2003). PMID 14676297.
original Tahara-Hanaoka, S., Lentiviral vector-mediated transduction of murine CD34(-) hematopoietic stem cells. Exp. Hematol. 30 (1): 11-17 (2002). PMID 11823032.
original Sumimoto, H., Rapid and efficient generation of lentivirally gene-modified dendritic cells from DC progenitors with bone marrow stromal cells. J. Immunol. Methods 271 (1-2): 153-165 (2002). PMID 12445738.
original Miyoshi, H., Development of a self-inactivating lentivirus vector. J. Virol., 72 (10): 8150-8157 (1998). PMID 9733856.

Further references such as user reports and related articles (go to bottom)

Featured content


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB17487_A9I6p1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Region: EGFP,CMV pro | Primer: EGFP-M_R | Sequence: RDB17487_A9I6e.seq
>04379_17487_A9I6_3_EGFP-M_R_A01_01_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNGGNNCTT GNNNTTNNCC TTGNTGCCGT TCTTCTGCTT
   61 GTCGGCCATG ATATANNNNT TGTGGCTGTT GTAGTTGTAC TCCAGCTTGT GCCCCAGGAT
  121 GTTGCCGTCC TCCTTGAAGT CGATGCCCTT CAGCTCGATG CGGTTCACCA GGGTGTCGCC
  181 CTCGAACTTC ACCTCGGCGC GGGTCTTGTA GTTGCCGTCG TCCTTGAAGA AGATGGTGCG
  241 CTCCTGGACG TAGCCTTCGG GCATGGCGGA CTTGAAGAAG TCGTGCTGCT TCATGTGGTC
  301 GGGGTAGCGG CTGAAGCACT GCACGCCGTA GGTCAGGGTG GTCACGAGGG TGGGCCAGGG
  361 CACGGGCAGC TTGCCGGTGG TGCAGATGAA CTTCAGGGTC AGCTTGCCGT AGGTGGCATC
  421 GCCCTCGCCC TCGCCGGACA CGCTGAACTT GTGGCCGTTT ACGTCGCCGT CCAGCTCGAC
  481 CAGGATGGGC ACCACCCCGG TGAACAGCTC CTCGCCCTTG CTCACCATGG TGGCGACCGG
  541 TAGCGCTAGC GGATCTGACG GTTCACTAAA CCAGCTCTGC TTATATAGAC CTCCCACCGT
  601 ACACGCCTAC CGCCCATTTG CGTCAATGGG GCGGAGTTGT TACGACATTT TGGAAAGTCC
  661 CGTTGATTTT GGTGCCAAAA CAAACTCCCA TTGACGTCAA TGGGGTGGAG ACTTGGAAAT
  721 CCCCGTGAGT CAAACCGCTA TCCACGCCCA TTGATGTACT GCCAAAACCG CATCANCATG
  781 GTAATAGCGA TGACTAATAC GTANATGTAC TGCCAAGTAN GAAAGTCCCA TAANGTCATG
  841 TACTGGGCAT AATGCNAGGC GGGCCATTTA NCGTCNTTGA CGTCNATAGG GGGCGTACTT
901 GGNCATATGA TACNCTTGAT GTACTGCC
//
Region: PRE,EGFP | Primer: PRE_rev | Sequence: RDB17487_A9I6f.seq
>04379_17487_A9I6_3_PRE_rev_B10_04_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNANCNNN ATAGTTAAGA ATACCAGTCA ATCTTTCNCA
   61 AATTTTGTAA TCCAGAGGTT GATTATCGAT AAGCTTGATC CCTCGAGATC CTACTAGTAG
  121 GATCTGAGTC CGGACTTGTA CAGCTCGTCC ATGCCGAGAG TGATCCCGGC GGCGGTCACG
  181 AACTCCAGCA GGACCATGTG ATCGCGCTTC TCGTTGGGGT CTTTGCTCAG GGCGGACTGG
  241 GTGCTCAGGT AGTGGTTGTC GGGCAGCAGC ACGGGGCCGT CGCCGATGGG GGTGTTCTGC
  301 TGGTAGTGGT CGGCGAGCTG CACGCTGCCG TCCTCGATGT TGTGGCGGAT CTTGAAGTTC
  361 ACCTTGATGC CGTTCTTCTG CTTGTCGGCC ATGATATAGA CGTTGTGGCT GTTGTAGTTG
  421 TACTCCAGCT TGTGCCCCAG GATGTTGCCG TCCTCCTTGA AGTCGATGCC CTTCAGCTCG
  481 ATGCGGTTCA CCAGGGTGTC GCCCTCGAAC TTCACCTCGG CGCGGGTCTT GTAGTTGCCG
  541 TCGTCCTTGA AGAAGATGGT GCGCTCCTGG ACGTAGCCTT CGGGCATGGC GGACTTGAAG
  601 AAGTCGTGCT GCTTCATGTG GTCGGGGTAG CGGCTGAAGC ACTGCACGCC GTAGGTCANG
  661 GTGGTCACGA GGGTGGGCCA GGGCACGGGC AGCTTGCCGG TGGTGCAGAT GAACTTCANG
  721 GTCAGCTTGC CGTANGTGGC ATCGCCCTCG CCCTCGCCGG ACACGCTGAA CTTGTGGCCG
  781 TTTACGTCGC CGTCCAGCTC GACCAGGATG GGCACCACCC CGGTGAACAG CTCCTCGCCC
841 TTGCTCACCA TGGTGGCGAC CGGTAGCGCT AGCGGATCTG ACGGTTCACT NANN
//
Region: HIV-1psi,5'LTR,CMVpro | Primer: HIV-B_R | Sequence: RDB17487_A9I6g.seq
>04379_17487_A9I6_3_HIV-B_R_C10_07_ABI24.ab1
    1 NNNNNNNNNN NNNNCNNNNN NNNNTTTTGG NGTACTCNCC NGTCGCCGCC CCTCGCCTCT
   61 TGCCGTGCGC GCTTCAGCAA GCCGAGTCCT GCGTCGAGAG AGCTCCTCTG GTTTCCCTTT
  121 CGCTTTCAAG TCCCTGTTCG GGCGCCACTG CTAGAGATTT TCCACACTGA CTAAAAGGGT
  181 CTGAGGGATC TCTAGTTACC AGAGTCACAC AACAGACGGG CACACACTAC TTGAAGCACT
  241 CAAGGCAAGC TTTATTGAGG CTTAAGCAGT GGGTTCCCTA GTTAGCCAGA GAGCTCCCAG
  301 GCTCAGATCT GGTCTAACCA GAGAGACCCA GTACAGGCAA AACGCGCTGC TTATATAGAC
  361 CTCCCACCGT ACACGCCTAC CGCCCATTTG CGTCAATGGG GCGGAGTTGT TACGACATTT
  421 TGGAAAGTCC CGTTGATTTT GGTGCCAAAA CAAACTCCCA TTGACGTCAA TGGGGTGGAG
  481 ACTTGGAAAT CCCCGTGAGT CAAACCGCTA TCCACGCCCA TTGATGTACT GCCAAAACCG
  541 CATCACCATG GTAATAGCGA TGACTAATAC GTAGATGTAC TGCCAAGTAG GAAAGTCCCA
  601 TAAGGTCATG TACTGGGCAT AATGCCAGGC GGGCCATTTA CCGTCATTGA CGTCAATAGG
  661 GGGCGTACTT GGCATATGAT ACACTTGATG TACTGCCAAG TGGGCAGTTT ACCGTAAATA
  721 CTCCACCCAT TGACGTCAAT GGAAAGTCCC TATTGGCGTT ACTATGGGAA CATACGTCAT
  781 TATTGACGTC AATGGGCGGG GGTCGTTGGG CGGTCAGCCA GGCGGGCCAT TTACCGTAAG
841 TTATGTAACG CGGAACTCCN TATATGGGCT ATGAACTAAT GN
//
Region: cPPT_CTS,RRE | Primer: cPPT/CTS-E_R | Sequence: RDB17487_A9I6h.seq
>04379_17487_A9I6_3_cPPT_CTS-E_R_D10_10_ABI24.ab1
    1 NNNNNNNNNT NGNTTTTGNA TTCTTTAGTT TGTATGTCTG TTGCTATTAT GTCTACTATT
   61 CTTTCCCCTG CACTGTACCC CCCAATCCCC CCTTTTCTTT TAAAATTGTG GATGAATACT
  121 GCCATTTGTA GATCCGTTCA CTAATCGAAT GGATCTGTCT CTGTCTCTCT CTCCACCTTC
  181 TTCTTCTATT CCTTCGGGCC TGTCGGGTCC CCTCGGGGTT GGGAGGTGGG TCTGAAACGA
  241 TAATGGTGAA TATCCCTGCC TAACTCTATT CACTATAGAA AGTACAGCAA AAACTATTCT
  301 TAAACCTACC AAGCCTCCTA CTATCATTAT GAATAATTTT ATATACCACA GCCAATTTGT
  361 TATGTTAAAC CAATTCCACA AACTTGCCCA TTTATCTAAT TCCAATAATT CTTGTTCATT
  421 CTTTTCTTGC TGGTTTTGCG ATTCTTCAAT TAAGGAGTGT ATTAAGCTTG TGTAATTGTT
  481 AATTTCTCTG TCCCACTCCA TCCAGGTCGT GTGATTCCAA ATCTGTTCCA GAGATTTATT
  541 ACTCCAACTA GCATTCCAAG GCACAGCAGT GGTGCAAATG AGTTTTCCAG AGCAACCCCA
  601 AATCCCCAGG AGCTGTTGAT CCTTTAGGTA TCTTTCCACA GCCAGGATTC TTGCCTGGAG
  661 CTGCTTGATG CCCCAGACTG TGAGTTGCAA CAGATGCTGT TGCGCCTCAA TAGCCCTCAG
  721 CAAATTGTTC TGCTGCTGCA CTATACCAGA CAATAATTGT CTGGCCTGTA CCGTCAGCGT
  781 CATTGACGCT GCGCCCATAG TGCTTCCTGC TGCTCCCNAG AACCCAAGGA ACAAAGCTCC
  841 TATTCCCACT GCTCTTTTTT CTCTCTGCAC CACTCTTCTC TTTGCCNTGG NGGGNGCTAC
901 TCCNAATGGT TCAATTTTTN ACTACTTNAT ATTTATATAA N
//
Region: bGHp(A),3'LTR | Primer: BGH_rev2 | Sequence: RDB17487_A9I6i.seq
>04379_17487_A9I6_3_BGH_rev2_E10_13_ABI24.ab1
    1 NNNNNNNNNN NNNNNNGGGG GNNGGGNNNA CNACAGATGG CTGGCAACTA GAAGGCACAG
   61 TCGAGGCTGA TCAGCGGGTT TAAACGGGCC CTGCTAGAGA TTTTCCACAC TGACTAAAAG
  121 GGTCTGAGGG ATCTCTAGTT ACCAGAGTCA CACAACAGAC GGGCACACAC TACTTGAAGC
  181 ACTCAAGGCA AGCTTTATTG AGGCTTAAGC AGTGGGTTCC CTAGTTAGCC AGAGAGCTCC
  241 CAGGCTCAGA TCTGGTCTAA CCAGAGAGAC CCAGTACAGT CCGGATGCAG CTCTCGGGCC
  301 ATGTGATGAA ATGCTAGGCG GCTGTCAAAC CTCCACTCTA ATACTTCTCT CTCCGGGTCA
  361 TCCATCCCAT GCAGGCTCAC AGGGTGTAAC AAGCGGGTGT TCTCTCCTTC ATTGGCTTCT
  421 TCTACCTTCT CTTGCTCAAC TGGTACTAGC TTGTAGCACC ATCCAAAGGT CAGTGGATAT
  481 CTGATCCCTG GCCCTGGTGT GTAGTTCTGC CAATCAGGGA AGTAGCCTTG TGTGTGGTAG
  541 ATCCACAGAT CAAGGATATC TTGTCTTCGT TGGGAGTGAA TTAGCCCTTC CAGTCCCCCC
  601 TTTTCTTTTA AAAAGTGGCT AAGATCTACA GCTGCCTTGT AAGTCATTGG TCTTAAAGGT
  661 ACCTGAGGTG TGACTGGAAA ACCCACCTCC TCCTCCTCTT GTGCTTCTAG CCAGGCACAA
  721 TCAGCATTGG TAGCTGCTGT ATTGCTACTT GTGATTGCTC CATGTTTTTC TAGGTCTCGA
781 CNGTATCGAT GCGGGGANGC GGNCCN
//
Region: Zeo,SV40pro_ori | Primer: SV40polyA_R2 | Sequence: RDB17487_A9I6j.seq
>04379_17487_A9I6_3_SV40polyA_R2_F10_16_ABI24.ab1
    1 NNNNNNNNNC NNNNNNNNNN NGTTGGGNNG GGCGAAGAAC TCCNGCATGA GATCCCCGCG
   61 CTGGAGGANC ATCCAGCCGG CGTCCCGGAA AACGATTCCG AAGCCCAACC TTTCATAGAA
  121 GGCGGCGGTG GAATCGAAAT CTCGTAGCAC GTGTCAGTCC TGCTCCTCGG CCACGAAGTG
  181 CACGCAGTTG CCGGCCGGGT CGCGCAGGGC GAACTCCCGC CCCCACGGCT GCTCGCCGAT
  241 CTCGGTCATG GCCGGCCCGG AGGCGTCCCG GAAGTTCGTG GACACGACCT CCGACCACTC
  301 GGCGTACAGC TCGTCCAGGC CGCGCACCCA CACCCAGGCC AGGGTGTTGT CCGGCACCAC
  361 CTGGTCCTGG ACCGCGCTGA TGAACAGGGT CACGTCGTCC CGGACCACAC CGGCGAAGTC
  421 GTCCTCCACG AAGTCCCGGG AGAACCCGAG CCGGTCGGTC CAGAACTCGA CCGCTCCGGC
  481 GACGTCGCGC GCGGTGAGCA CCGGAACGGC ACTGGTCAAC TTGGCCATGG TTTAGTTCCT
  541 CACCTTGTCG TATTATACTA TGCCGATATA CTATGCCGAT GATTAATTGT CAACACGTGC
  601 TGATCAGATC CGAAAATGGA TATACAAGCT CCCGGGAGCT TTTTGCAAAA GCCTAGGCCT
  661 CCAAAAAAGC CTCCTCACTA CTTCTGGAAT AGCTCAGAGG CAGAGGCGGC CTCGGCCTCT
721 GCATAAATAA AAAAAATTAG TCAGCCATGG GGCGGANAAT
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Shibuya, K., CD226 (DNAM-1) is involved in lymphocyte function-associated antigen 1 costimulatory signal for naive T cell differentiation and proliferation. J. Exp. Med. 198 (12): 1829-1839 (2003). PMID 14676297.
original Tahara-Hanaoka, S., Lentiviral vector-mediated transduction of murine CD34(-) hematopoietic stem cells. Exp. Hematol. 30 (1): 11-17 (2002). PMID 11823032.
original Sumimoto, H., Rapid and efficient generation of lentivirally gene-modified dendritic cells from DC progenitors with bone marrow stromal cells. J. Immunol. Methods 271 (1-2): 153-165 (2002). PMID 12445738.
original Miyoshi, H., Development of a self-inactivating lentivirus vector. J. Virol., 72 (10): 8150-8157 (1998). PMID 9733856.
reference Yamaguchi, T., Comparison of gene-trapping efficiency between retroviral and lentiviral vectors in mouse embryonic stem cells. Biochem. Biophys. Res. Commun. 425 (2): 297-303 (2012). PMID 22842569.
reference , Effective transduction and stable transgene expression in human blood cells by a third-generation lentiviral vector. Gene Ther. 10 (17): 1446-1457 (2003). PMID 12900759.
user report Suzuki, E., Secretion of high amounts of hepatocyte growth factor is a characteristic feature of cancer-associated fibroblasts with EGFR-TKI resistance-promoting phenotype: A study of 18 cases of cancer-associated fibroblasts. Pathol. Int. 69 (8): 472-480 (2019). PMID 31339210.
user report Ogawa, Y., Elavl3 is essential for the maintenance of Purkinje neuron axons. Sci. Rep. 8 (1): 2722 (2018). PMID 29426875.
user report Shamoto, N., CFAP70 Is a Novel Axoneme-Binding Protein That Localizes at the Base of the Outer Dynein Arm and Regulates Ciliary Motility. Cells 7 (9). pii: E124 (2018). PMID 30158508.
user report Katsura, A., ZEB1-regulated inflammatory phenotype in breast cancer cells. Mol. Oncol. 11 (9): 1241-1262 (2017). PMID 28618162.
user report Suzuki, S., Characterization of actions of octanoate on porcine preadipocytes and adipocytes differentiated in vitro. Biochem. Biophys. Res. Commun., 432 (1): 92-98 (2013). PMID 23376076.
user report Yaguchi, M., Characterization of the properties of seven promoters in the motor cortex of rats and monkeys after lentiviral vector-mediated gene transfer. Hum. Gene Ther. Methods, 24 (6): 333-344 (2013). PMID 23964981.
user report Su, S., Genetically encoded calcium indicator illuminates calcium dynamics in primary cilia. Nat. Methods, 10 (11): 1105-1107 (2013). PMID 24056873.
user report Tamura, K., A glass-coated tungsten microelectrode enclosing optical fibers for optogenetic exploration in primate deep brain structures. J. Neurosci. Methods, 211 (1): 49-57 (2012). PMID 22971353.
user report Saitoh, Y., Overexpression of NF-kappaB inducing kinase underlies constitutive NF-kappaB activation in lung cancer cells. Lung. Cancer., 70, 263-270 (2010). PMID 20338663.
user report Ohashi Y., A bicistronic lentiviral vector-based method for differential transsynaptic tracing of neural circuits. Mol. Cell. Neurosci. 46 (1): 136-147. PMID 20816792.
user report Barnor, J.S., Inhibition of HIV-1 replication by long-term treatment with a chimeric RNA containing shRNA and TAR decoy RNA. Antiviral Res. 83 (2): 156-164 (2009). PMID 19397929.
user report Hanyu, A., Functional in vivo optical imaging of tumor angiogenesis, growth, and metastasis prevented by administration of anti-human VEGF antibody in xenograft model of human fibrosarcoma HT1080 cells. Cancer Sci. 100 (11): 2085-2092 (2009). PMID 19719773.
user report Yoshida, S., Knockdown of synapse-associated protein Dlg1 reduces syncytium formation induced by human T-cell leukemia virus type 1. Virus Genes, 37 (1): 9-15 (2008). PMID 18461433.
user report Inoue-Narita, T., Pten deficiency in melanocytes results in resistance to hair graying and susceptibility to carcinogen-induced melanomagenesis. Cancer Res. 2008 Jul 15;68(14):5760-8 (2008). PMID 18632629.
user report Ehata, S., Ki26894, a novel transforming growth factor-beta type I receptor kinase inhibitor, inhibits in vitro invasion and in vivo bone metastasis of a human breast cancer cell line. Cancer Sci. 98 (1): 127-133 (2007). PMID 17129361.
user report Tanaka, T., Steroidogenic factor 1/adrenal 4 binding protein transforms human bone marrow mesenchymal cells into steroidogenic cells. J. Mol. Endocrinol., 39 (5): 343-350 (2007). PMID 17975261.
user report Lu F.Z., Long-term gene expression using the lentiviral vector in rat chondrocytes. Clin. Orthop. Relat. Res., 439, 243-252 (2005). PMID 16205166.
user report Lu F.Z., Characterization and gene transfer in mesenchymal stem cells derived from human umbilical-cord blood. J. Lab. Clin. Med., 146, 271-278 (2005). PMID 16242526.

2019.10.05

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