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CSII-EF-MCS

Backbone vector plasmid of lentivirus construct with EF-1 alpha promoter.

Catalog number RDB04378
Resource name CSII-EF-MCS
Alternative name pCSII-EF-MCS
Clone info. Backbone vector plasmid of lentivirus construct with EF-1 alpha promoter.
Comment Commonly requested with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394).
Vector backbone Plasmid
Selectable markers Ampicillin (E. coli). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Depositor|Developer Miyoshi, Hiroyuki |
 
Sequence (full) RDB04378hts01.seqcheck assembled from experimentally sequenced data
Remarks, protocol and/or map (pdf) RDB04378.pdf
Test sheet RDB17471_A9Hlp1-3.pdf 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions for distribution The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer.
Ordering
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MTA, for use for not-for-profit academic purpose)[Word]
Please visit Ordering instruction.[link] 
Remarks ((Remarks)) Remember that you will be working with samples containing infectious virus.
提供案内 (日本国内) [open/close]

提供条件 本件研究材料は、非営利機関の非営利学術研究に限って提供する。本件研究材料を利用した研究結果等を発表する際は、本件研究材料が故三好浩之博士により開発されたことを明示する。
提供依頼
提供依頼書 [Word]
提供同意書 (MTA, 非営利機関による非営利学術研究用)[Word]
遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。
手続きの概要は、「レンチウイルスベクターの提供申し込み」をご覧ください。
備考 【使用上の注意】このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB04378 CSII-EF-MCS DNA solution

Ordering instruction of plasmids [in Japanese] [in English]

How to cite this biological resource

Materials & Methods section:

The CSII-EF-MCS was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB04378).

Reference section:

Yamaguchi, T., Morikawa, A., Miyoshi, H., Comparison of gene-trapping efficiency between retroviral and lentiviral vectors in mouse embryonic stem cells. Biochem. Biophys. Res. Commun. 425 (2): 297-303 (2012). PMID 22842569. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)


References

Original, user report and related articles

original Yamaguchi, T., Comparison of gene-trapping efficiency between retroviral and lentiviral vectors in mouse embryonic stem cells. Biochem. Biophys. Res. Commun. 425 (2): 297-303 (2012). PMID 22842569. [link to RRC of NBRP]
user_report Miyamoto, M., Theilovirus 3C Protease Cleaves the C-Terminal Domain of the Innate Immune RNA Sensor, Melanoma Differentiation-Associated Gene 5, and Impairs Double-Stranded RNA-Mediated IFN Response. J. Immunol. 210 (3): 335-347 (2023). PMID 36525065. [link to RRC of NBRP]
user_report Watanabe, K., Myeloid-associated differentiation marker is an essential host factor for human parechovirus PeV-A3 entry. Nat. Commun. 14 (1): 1817 (2023). PMID 37002207. [link to RRC of NBRP]
user_report Sakai, E., miR-27b targets MAIP1 to mediate lipid accumulation in cultured human and mouse hepatic cells. Commun. Biol. 6 (1): 669 (2023). PMID 37355744. [link to RRC of NBRP]
user_report Iriki, T., Senescent cells form nuclear foci that contain the 26S proteasome. Cell Rep. 42 (8): 112880 (2023). PMID 37541257. [link to RRC of NBRP]
user_report Hayashi, Y., Nutritional and metabolic control of germ cell fate through O-GlcNAc regulation. EMBO Rep. 24 (11):
e56845 (2023). PMID 37842859. [link to RRC of NBRP]
user_report Hirano, M., A highly photostable and bright green fluorescent protein. Nat. Biotechnol. 40 (7): 1132-1142 (2022). PMID 35468954. [link to RRC of NBRP]
user_report Kanatsu-Shinohara, M., Adeno-associated-virus-mediated gene delivery to ovaries restores fertility in congenital infertile mice. Cell Rep. Med. 3 (5): 100606 (2022) PMID 35584625. [link to RRC of NBRP]
user_report Takizawa, M., Development of a red fluorescent protein-based cGMP indicator applicable for live-cell imaging. Commun. Biol. 5 (1): 833 (2022). PMID 36064581. [link to RRC of NBRP]
user_report Hino, Y., Mitochondrial stress induces AREG expression and epigenomic remodeling through c-JUN and YAP-mediated enhancer activation. Nucleic Acids Res. 50 (17): 9765-9779 (2022). PMID 36095121. [link to RRC of NBRP]
user_report Meshitsuka, S., CRISPR/Cas9 and AAV mediated insertion of β2 microglobulin-HLA-G fusion gene protects mesenchymal stromal cells from allogeneic rejection and potentiates the use for off-the-shelf cell therapy. Regen. Ther. 21: 442-452 (2022). PMID 36313397. [link to RRC of NBRP]
user_report Morimoto, A., SLPI is a critical mediator that controls PTH-induced bone formation. Nat. Commun. 12 (1): 2136 (2021). PMID 33837198. [link to RRC of NBRP]
user_report Kondo, N., Kindlin-3 disrupts an intersubunit association in the integrin LFA1 to trigger positive feedback activation by Rap1 and talin1. Sci. Signal. 14 (686): eabf2184 (2021). PMID 34103420. [link to RRC of NBRP]
user_report Sima, F., Mimicking Intravasation–Extravasation with a 3D Glass Nanofluidic Model for the Chemotaxis‐Free Migration of Cancer Cells in Confined Spaces. Adv. mater. Technol. 5: 2000484 (2020). [link to RRC of NBRP]
user_report Nakamura, T., Tissue-nonspecific alkaline phosphatase promotes the osteogenic differentiation of osteoprogenitor cells. Biochem. Biophys. Res. Commun. 524 (3): 702-709 (2020). PMID 32035618. [link to RRC of NBRP]
user_report Imajo, M., Analysis of Retinoic Acid Receptor Signaling in Colorectal Cancer. Methods Mol. Biol. 2019: 85-93 (2019). PMID 31359390. [link to RRC of NBRP]
user_report Nakashima, M., Trogocytosis of ligand-receptor complex and its intracellular transport in CD30 signalling. Biol. Cell 110 (5): 109-124 (2018). PMID 29431186. [link to RRC of NBRP]
user_report Chiyonobu, N., Fatty Acid Binding Protein 4 (FABP4) Overexpression in Intratumoral Hepatic Stellate Cells within Hepatocellular Carcinoma with Metabolic Risk Factors. Am. J. Pathol. 188 (5): 1213-1224 (2018). PMID 29454748. [link to RRC of NBRP]
user_report Kasahara, K., EGF receptor kinase suppresses ciliogenesis through activation of USP8 deubiquitinase. Nat. Commun. 9 (1): 758 (2018). PMID 29472535. [link to RRC of NBRP]
user_report Ueda, N., Generation of TCR-Expressing Innate Lymphoid-like Helper Cells that Induce Cytotoxic T Cell-Mediated Anti-leukemic Cell Response. Stem Cell Reports 10 (6): 1935-1946 (2018). PMID 29805109. [link to RRC of NBRP]
user_report Miura, H., Cell-to-Cell Heterogeneity in p38-Mediated Cross-Inhibition of JNK Causes Stochastic Cell Death. Cell Rep. 24 (10): 2658-2668 (2018). PMID 30184500. [link to RRC of NBRP]
user_report Mochizuki, K., Repression of Somatic Genes by Selective Recruitment of HDAC3 by BLIMP1 Is Essential for Mouse Primordial Germ Cell Fate Determination. Cell Rep. 24 (10): 2682-2693.e6 (2018). PMID 30184502. [link to RRC of NBRP]
user_report Yanagihashi, Y., Mouse macrophages show different requirements for phosphatidylserine receptor Tim4 in efferocytosis. Proc. Natl. Acad. Sci. U.S.A. 114 (33): 8800-8805 (2017). PMID 28768810. [link to RRC of NBRP]
user_report Nakamori, D., Direct conversion of human fibroblasts into hepatocyte-like cells by ATF5, PROX1, FOXA2, FOXA3, and HNF4A transduction. Sci. Rep. 7 (1): 16675 (2017). PMID 29192290. [link to RRC of NBRP]
user_report Otani, K., Cell-cycle-controlled radiation therapy was effective for treating a murine malignant melanoma cell line in vitro and in vivo. Sci. Rep. 6: 30689 (2016). PMID 27480052. [link to RRC of NBRP]
user_report Shcherbakova, D.M., Bright monomeric near-infrared fluorescent proteins as tags and biosensors for multiscale imaging. Nat. Commun. 7: 12405 (2016). PMID 27539380. [link to RRC of NBRP]
user_report Matsu-Ura, T., Intercellular Coupling of the Cell Cycle and Circadian Clock in Adult Stem Cell Culture. Mol. Cell 64 (5): 900-912 (2016). PMID 27867006. [link to RRC of NBRP]
user_report Naito, A., RFPL4A increases the G1 population and decreases sensitivity to chemotherapy in human colorectal cancer cells. J Biol Chem. 2015 Jan 20. pii: jbc.M114.614859 PMID 25605732. [link to RRC of NBRP]
user_report Takahashi, H., Significance of Polypyrimidine Tract-Binding Protein 1 Expression in Colorectal Cancer. Mol. Cancer. Ther. 14 (7): 1705-1716 (2015). PMID 25904505. [link to RRC of NBRP]
user_report Sato, Y., Disease modeling and lentiviral gene transfer in patient-specific induced pluripotent stem cells from late-onset Pompe disease patient. Mol. Ther. Methods Clin. Dev. 2: 15023 (2015). PMID 26199952. [link to RRC of NBRP]
user_report Kim, Y.C., Androgen Receptor-Mediated Growth Suppression of HPr-1AR and PC3-Lenti-AR Prostate Epithelial Cells. PLoS One 10 (9): e0138286 (2015). PMID 26372468. [link to RRC of NBRP]
user_report Morishima T., Genetic correction of HAX1 in induced pluripotent stem cells from a patient with severe congenital neutropenia improves defective granulopoiesis. Haematologica 99 (1): 19-27 (2014). PMID 23975175. [link to RRC of NBRP]
user_report Matsumoto, S., A combination of Wnt and growth factor signaling induces Arl4c expression to form epithelial tubular structures. EMBO J., 33 (7): 702-718 (2014). PMID 24562386. [link to RRC of NBRP]
user_report Maeda, S., Interferon-alpha Acts on the S/G2/M Phases to Induce Apoptosis in the G1 Phase of an IFNAR2-Expressing Hepatocellular Carcinoma Cell Line. J. Biol. Chem. 289 (34): 23786-23795 (2014). PMID 25012666. [link to RRC of NBRP]
user_report Kojima, K., ESCRT-0 Protein Hepatocyte Growth Factor-regulated Tyrosine Kinase Substrate (Hrs) Is Targeted to Endosomes Independently of Signal-transducing Adaptor Molecule (STAM) and the Complex Formation with STAM Promotes Its Endosomal Dissociation. J. Biol. Chem. 289 (48): 33296-33310 (2014). PMID 25296754. [link to RRC of NBRP]
user_report Kondo, S., An HLA-modified ovarian cancer cell line induced CTL responses specific to an epitope derived from claudin-1 presented by HLA-A*24:02 molecules. Hum. Immunol., 74 (9): 1103-1110 (2013). PMID 23806269. [link to RRC of NBRP]
user_report Inaguma, S., GLI1 Interferes with the DNA Mismatch Repair System in Pancreatic Cancer through BHLHE41-Mediated Suppression of MLH1. Cancer Res., 73 (24): 7313-7323 (2013). PMID 24165159. [link to RRC of NBRP]
user_report Kagawa, Y., Cell Cycle-Dependent Rho GTPase Activity Dynamically Regulates Cancer Cell Motility and Invasion In Vivo. PLoS One, 8 (12): e83629 (2013). PMID 24386239. [link to RRC of NBRP]
user_report Toyomitsu, E., CCL2 promotes P2X4 receptor trafficking to the cell surface of microglia. Purinerg. Signal, 8 (2): 301-310 (2012). PMID 22222817. [link to RRC of NBRP]
user_report Hirota, Y., Roles of planar cell polarity signaling in maturation of neuronal precursor cells in the postnatal mouse olfactory bulb. Stem Cells, 30 (8): 1726-1733 (2012).無 PMID 22628050. [link to RRC of NBRP]
user_report Masuda, T., IRF8 Is a Critical Transcription Factor for Transforming Microglia into a Reactive Phenotype. Cell Rep., 1 (4): 334-340 (2012). PMID 22832225. [link to RRC of NBRP]
user_report Sakaue-Sawano, A., Drug-induced cell cycle modulation leading to cell-cycle arrest, nuclear mis-segregation, or endoreplication. BMC Cell Biol. 12: 2 (2011). PMID 21226962. [link to RRC of NBRP]
user_report Amako, Y., Protein kinase D negatively regulates hepatitis C virus secretion through phosphorylation of oxysterol-binding protein and ceramide transfer protein. J. Biol. Chem., 286 (13): 11265-11274 (2011). PMID 21285358. [link to RRC of NBRP]
user_report Ibi, M., Trichoplein controls microtubule anchoring at the centrosome by binding to Odf2 and ninein. J. Cell Sci., 124 (Pt 6): 857-864 (2011). PMID 21325031. [link to RRC of NBRP]
user_report Hirota Y., Planar polarity of multiciliated ependymal cells involves the anterior migration of basal bodies regulated by non-muscle myosin II. Development, 137, 3037-3046 (2010). PMID 20685736. [link to RRC of NBRP]
user_report Sasai, M., Bifurcation of Toll-like receptor 9 signaling by adaptor protein 3. Science 329 (5998): 1530-1534 (2010). PMID 20847273. [link to RRC of NBRP]
user_report Kiriyama, M., Interaction of FLASH with arsenite resistance protein 2 is involved in cell cycle progression at S phase. Mol. Cell. Biol., 29, 4729-4741 (2009). PMID 19546234. [link to RRC of NBRP]
user_report Shinoda Y., Efficient transduction of cytotoxic and anti-HIV-1 genes by a gene-regulatable lentiviral vector. Virus Genes., 39, 165-175 (2009). PMID 19554442. [link to RRC of NBRP]
user_report Isogawa, M., Rearranged NF-kappa B2 gene in an adult T-cell leukemia cell line. Cancer Sci., 99 (4): 792-798 (2008). PMID 18377428. [link to RRC of NBRP]
user_report Higuchi, M., Cooperation of NF-kappaB2/p100 activation and the PDZ domain binding motif signal in human T-cell leukemia virus type 1 (HTLV-1) Tax1 but not HTLV-2 Tax2 is crucial for interleukin-2-independent growth transformation of a T-cell line. J. Virol. 81 (21): 11900-11907 (2007). PMID 17715223. [link to RRC of NBRP]