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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

CSII-EF-MCS

Backbone vector plasmid of lentivirus construct with EF-1 alpha promoter.

Catalog number RDB04378
Resource name CSII-EF-MCS
Alternative name pCSII-EF-MCS
Clone info. Backbone vector plasmid of lentivirus construct with EF-1 alpha promoter.
Commonly requested with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Vector backbone Plasmid
Selectable markers Please note that Zeo resistance marker is not included in the lentivirus produced from this vector. E. coli: Amp^r.
Depositor|Developer Miyoshi, Hiroyuki |
Lentiviral plasmid list, maps and sequences information. [link]

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer.
Ordering Please visit Ordering instruction.[link] 
Order form [Credit Card Payment]  [Bank Transfer or Check Payment]
Material Transfer Agreement (MTA, for use for not-for-profit academic purpose)[Word]
Remarks ((Remarks)) Remember that you will be working with samples containing infectious virus.
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB04378 CSII-EF-MCS DNA solution

Ordering instruction of plasmids [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Electronic file Sequence RDB04378z.seq
Electronic file Remarks, protocol and/or map (pdf) RDB04378.pdf

Original reference

original Yamaguchi, T., Comparison of gene-trapping efficiency between retroviral and lentiviral vectors in mouse embryonic stem cells. Biochem. Biophys. Res. Commun. 425 (2): 297-303 (2012). PMID 22842569.

Further references such as user reports and related articles (go to bottom)

Featured content

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB17471_A9Hlp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: EF1a-C_F
Region: EF1 alpha pro,MCS,PRE
Sequence file: RDB17471_A9Hla.seq check
>04378_17471_A9Hl_EF1a-C_F_B03_06_ABI24.ab1
    1 NNNNNNNNNN NNNNTNTTGA NTTTGGNNCT TGGTTNNTTC TCNAGCCTCA GACAGTGGTT
   61 CAAAGTTTTT TTCTTCCATT TCAGGTGTCG TGAACACGCT ACCGGTCTCG AGAATTCTGC
  121 AGCGGCCGCG GATCCTCTAG AGTTAACATC GAGGGATCAA GCTTATCGAT AATCAACCTC
  181 TGGATTACAA AATTTGTGAA AGATTGACTG GTATTCTTAA CTATGTTGCT CCTTTTACGC
  241 TATGTGGATA CGCTGCTTTA ATGCCTTTGT ATCATGCTAT TGCTTCCCGT ATGGCTTTCA
  301 TTTTCTCCTC CTTGTATAAA TCCTGGTTGC TGTCTCTTTA TGAGGAGTTG TGGCCCGTTG
  361 TCAGGCAACG TGGCGTGGTG TGCACTGTGT TTGCTGACGC AACCCCCACT GGTTGGGGCA
  421 TTGCCACCAC CTGTCAGCTC CTTTCCGGGA CTTTCGCTTT CCCCCTCCCT ATTGCCACGG
  481 CGGAACTCAT CGCCGCCTGC CTTGCCCGCT GCTGGACAGG GGCTCGGCTG TTGGGCACTG
  541 ACAATTCCGT GGTGTTGTCG GGGAAGCTGA CGTCCTTTCC ATGGCTGCTC GCCTGTGTTG
  601 CCACCTGGAT TCTGCGCGGG ACGTCCTTCT GCTACGTCCC TTCGGCCCTC AATCCAGCGG
  661 ACCTTCCTTC CCGCGGCCTG CTGCCGGCTC TGCGGCCTCT TCCGCGTCTT CGCCTTCGCC
  721 CTCAGACGAG TCGGATCTCC CTTTGGGCCG CCTCCCCGCA TCGATACCGT CGANACCTAN
  781 AAAAACATGG AGCAATCACA AGTAGCAATA CAGCAGCTAC CAATGCTGAT TGTGCCTGGC
  841 TAGAANCACA AGANGANGAN GANGTGGGTT TTCCAGNCNC ACCTCANGNN NCTTTAAGAN
  901 NNATGNNTTA CNNGGNANNN GNANNTCTTA NCCNCTTTTT AAAANAAANN GGGGGNNNNN
961 NNNNN
//
Primer: BGH_rev2
Region: 3'LTR,PRE
Sequence file: RDB17471_A9Hlb.seq check
>04378_17471_A9Hl_BGH_rev2_A03_03_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNGNNGG GCNNANNACA GANGGCTGGC AACTAGAAGG
   61 CACAGTCGAG GCTGNTCAGC GGGTTTAAAC GGGCCCTGCT AGAGATTTTC CACACTGACT
  121 AAAAGGGTCT GAGGGATCTC TAGTTACCAG AGTCACACAA CAGACGGGCA CACACTACTT
  181 GAAGCACTCA AGGCAAGCTT TATTGAGGCT TAAGCAGTGG GTTCCCTAGT TAGCCAGAGA
  241 GCTCCCAGGC TCAGATCTGG TCTAACCAGA GAGACCCAGT ACAGTCCGGA TGCAGCTCTC
  301 GGGCCATGTG ATGAAATGCT AGGCGGCTGT CAAACCTCCA CTCTAATACT TCTCTCTCCG
  361 GGTCATCCAT CCCATGCAGG CTCACAGGGT GTAACAAGCG GGTGTTCTCT CCTTCATTGG
  421 CTTCTTCTAC CTTCTCTTGC TCAACTGGTA CTAGCTTGTA GCACCATCCA AAGGTCAGTG
  481 GATATCTGAT CCCTGGCCCT GGTGTGTAGT TCTGCCAATC AGGGAAGTAG CCTTGTGTGT
  541 GGTAGATCCA CAGATCAAGG ATATCTTGTC TTCGTTGGGA GTGAATTAGC CCTTCCAGTC
  601 CCCCCTTTTC TTTTAAAAAG TGGCTAAGAT CTACAGCTGC CTTGTAAGTC ATTGGTCTTA
  661 AAGGTACCTG AGGTGTGACT GGAAAACCCA CCTCCTCCTC CTCTTGTGCT TCTAGCCAGG
  721 CACAATCAGC ATTGGTAGCT GCTGTATTGC TACTTGTGAT TGCTCCATGT TTTTCTAGGT
  781 CTCGACGGTA TCGATGCGGG GAGGCGGCCC NAAGGGAGAT CCGANTCNTC TGANGGCGAA
  841 NGCGAANANG NNGAANANNN CGCANANCCN GCANCNNGCC GCGGGAANGA ANGTCCGCNN
  901 GATTGANGGN CGNANNGNNN NTANCANAAN GNNNTCCNGN GCANNNNNCN NGNNGCNNCA
961 CNGGCNANCN NNNNNNNNN
//
Primer: CMV_Forward
Region: CMV pro,5'LTR,HIV-1 psi
Sequence file: RDB17471_A9Hlc.seq check
>04387_17471_A9Hl_CMV_Forward_H01_22_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNTGNNGCN AATGGGCGGN NNGCGTGTAC GGTGGGAGGT
   61 CTATNNTAAG CAGCGCGTTT TGCCTGTACT GGGTCTCTCT GGTTAGACCA GATCTGAGCC
  121 TGGGAGCTCT CTGGCTAACT AGGGAACCCA CTGCTTAAGC CTCAATAAAG CTTGCCTTGA
  181 GTGCTTCAAG TAGTGTGTGC CCGTCTGTTG TGTGACTCTG GTAACTAGAG ATCCCTCAGA
  241 CCCTTTTAGT CAGTGTGGAA AATCTCTAGC AGTGGCGCCC GAACAGGGAC TTGAAAGCGA
  301 AAGGGAAACC AGAGGAGCTC TCTCGACGCA GGACTCGGCT TGCTGAAGCG CGCACGGCAA
  361 GAGGCGAGGG GCGGCGACTG GTGAGTACGC CAAAAATTTT GACTAGCGGA GGCTAGAAGG
  421 AGAGAGATGG GTGCGAGAGC GTCAGTATTA AGCGGGGGAG AATTAGATCG CGATGGGAAA
  481 AAATTCGGTT AAGGCCAGGG GGAAAGAAAA AATATAAATT AAAACATATA GTATGGGCAA
  541 GCAGGGAGCT AGAACGATTC GCAGTTAATC CTGGCCTGTT AGAAACATCA GAAGGCTGTA
  601 GACAAATACT GGGACAGCTA CAACCATCCC TTCAGACAGG ATCAGAAGAA CTTAGATCAT
  661 TATATAATAC AGTAGCAACC CTCTATTGTG TGCATCAAAG GATAGAGATA AAAGACACCA
  721 AGGAAGCTTT AGACAAGATA GAGGAAGAGC NAAACAAAAG TAAGANCACC GCACAGCAAG
  781 GGCCGCTGAT CTTCAGACCT GGANGANGAG ATATGANGGA NAATTGGANN AGTGAATTAT
  841 ATAANTATAA ANGTAGTAAA AATTGANCNN TTAGGAGTAN CNCCCNCCAA GGCNAAGAGA
  901 AGANTGNNGC NNAGAGAAAA AAGAGCANNG GGAATANGAN CTTTGTNNCN TGGGTTCTNG
961 GNANNANCNN GANNCNCTNT GGGCNCAGCG NCNNTNNNNN CTGANNNNNN NNNN
//
Primer: EF1a-N_R
Region: EF1 alpha pro,cPPT_CTS,RRE
Sequence file: RDB17471_A9Hld.seq check
>04387_17471_A9Hl_EF1a-N_R_D02_11_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN GCGNNGTGCG CTCTGCCNNC TGACGGGCAC CGGAGCCTCA
   61 CGAAGCTTGA ATTAATTCAA AGTGAATTTC TGCTGTCCCT GTAATAAACC CGAAAATTTT
  121 GAATTTTTGT AATTTGTTTT TGTAATTCTT TAGTTTGTAT GTCTGTTGCT ATTATGTCTA
  181 CTATTCTTTC CCCTGCACTG TACCCCCCAA TCCCCCCTTT TCTTTTAAAA TTGTGGATGA
  241 ATACTGCCAT TTGTAGATCC GTTCACTAAT CGAATGGATC TGTCTCTGTC TCTCTCTCCA
  301 CCTTCTTCTT CTATTCCTTC GGGCCTGTCG GGTCCCCTCG GGGTTGGGAG GTGGGTCTGA
  361 AACGATAATG GTGAATATCC CTGCCTAACT CTATTCACTA TAGAAAGTAC AGCAAAAACT
  421 ATTCTTAAAC CTACCAAGCC TCCTACTATC ATTATGAATA ATTTTATATA CCACAGCCAA
  481 TTTGTTATGT TAAACCAATT CCACAAACTT GCCCATTTAT CTAATTCCAA TAATTCTTGT
  541 TCATTCTTTT CTTGCTGGTT TTGCGATTCT TCAATTAAGG AGTGTATTAA GCTTGTGTAA
  601 TTGTTAATTT CTCTGTCCCA CTCCATCCAG GTCGTGTGAT TCCAAATCTG TTCCAGAGAT
  661 TTATTACTCC AACTAGCATT CCAAGGCACA GCAGTGGTGC AAATGAGTTT TCCAGAGCAA
  721 CCCCAAATCC CCAGGAGCTG TTGATCCTTT AGGTATCTTT CCACAGCCAG GANTCTTGCC
  781 TGGAGCTGCT TGATGCCCCA GACTGTGAGT TGCAACAGAT GCTGTTGCGC CTCAATAGCC
  841 CTCAGCAAAT TGNTCTGCTG CTGCACTATA CCAGANAATA ATTGTCTGGN CTGTANCGNC
  901 AGCGTCNTTG ACGCTGCGCC CATAGNGCTT NCTGCTGCTN CNNAGAACCN NNNNAANAAA
  961 GCTNCNATTC CCNCTGCTCT TTTTTCNCTC TGCANNNNTN NNCNCTTNGN CNTNNNNGGN
1021 NNNNNNNNN
//
Primer: Zeo-M_R
Region: Zeo,SV40 pro_ori
Sequence file: RDB17471_A9Hle.seq check
>04378_17471_A9Hl_Zeo-M_R_B03_02_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN CCCNCNCCCN GGCCAGGGTG TTGTCCGGCA
   61 CCACCTGGTC CTGGACCGCG CTGNTGAACA GGGTCACGTC GTCCCGGACC ACACCGGCGA
  121 AGTCGTCCTC CACGAAGTCC CGGGAGAACC CGAGCCGGTC GGTCCAGAAC TCGACCGCTC
  181 CGGCGACGTC GCGCGCGGTG AGCACCGGAA CGGCACTGGT CAACTTGGCC ATGGTTTAGT
  241 TCCTCACCTT GTCGTATTAT ACTATGCCGA TATACTATGC CGATGATTAA TTGTCAACAC
  301 GTGCTGATCA GATCCGAAAA TGGATATACA AGCTCCCGGG AGCTTTTTGC AAAAGCCTAG
  361 GCCTCCAAAA AAGCCTCCTC ACTACTTCTG GAATAGCTCA GAGGCAGAGG CGGCCTCGGC
  421 CTCTGCATAA ATAAAAAAAA TTAGTCAGCC ATGGGGCGGA GAATGGGCGG AACTGGGCGG
  481 AGTTAGGGGC GGGATGGGCG GAGTTAGGGG CGGGACTATG GTTGCTGACT AATTGAGATG
  541 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC TGGTTGCTGA
  601 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA
  661 CCCTAACTGA CACACATTCC ACAGAATTAA TTCGCGTTAA ATTTTTGTTA AATCAGCTCA
  721 TTTTTTAACC AATAGGCCGA AATCGGCAAA ATCCCTTATA AATCAAAAGA NTAGACCGAG
  781 ATANGGTTGA GTGTTGTTCC NGTTTGGAAC AAGAGTCCAC TATTAAAGAA CGTGGNACTN
  841 NNACGTCNAA GGGCGAAAAA CCNTCTATCN NGGCGATGGN CCCACTACGT GAACNATCAC
  901 CCTAATCAAG TTTTTTTGGG GNNCGANGNT GCCGTAAAGC ACTANNNCGN AACNNTNNNN
  961 GGGNNNNCCC CNGATTTANA NNNTNGANNG GNNAANNCNN NNNANNGTNG NNNANNAANG
1021 NANNNNNNAA AGCNNANNNA NCNGGGNNNN NNNGGNNNNN NNGNNNNNNN NNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Yamaguchi, T., Comparison of gene-trapping efficiency between retroviral and lentiviral vectors in mouse embryonic stem cells. Biochem. Biophys. Res. Commun. 425 (2): 297-303 (2012). PMID 22842569.
user report Sima, F., Mimicking Intravasation–Extravasation with a 3D Glass Nanofluidic Model for the Chemotaxis‐Free Migration of Cancer Cells in Confined Spaces. Adv. mater. Technol. 5: 2000484 (2020).
user report Nakamura, T., Tissue-nonspecific alkaline phosphatase promotes the osteogenic differentiation of osteoprogenitor cells. Biochem. Biophys. Res. Commun. 524 (3): 702-709 (2020). PMID 32035618.
user report Nakashima, M., Trogocytosis of ligand-receptor complex and its intracellular transport in CD30 signalling. Biol. Cell 110 (5): 109-124 (2018). PMID 29431186.
user report Chiyonobu, N., Fatty Acid Binding Protein 4 (FABP4) Overexpression in Intratumoral Hepatic Stellate Cells within Hepatocellular Carcinoma with Metabolic Risk Factors. Am. J. Pathol. 188 (5): 1213-1224 (2018). PMID 29454748.
user report Kasahara, K., EGF receptor kinase suppresses ciliogenesis through activation of USP8 deubiquitinase. Nat. Commun. 9 (1): 758 (2018). PMID 29472535.
user report Ueda, N., Generation of TCR-Expressing Innate Lymphoid-like Helper Cells that Induce Cytotoxic T Cell-Mediated Anti-leukemic Cell Response. Stem Cell Reports 10 (6): 1935-1946 (2018). PMID 29805109.
user report Miura, H., Cell-to-Cell Heterogeneity in p38-Mediated Cross-Inhibition of JNK Causes Stochastic Cell Death. Cell Rep. 24 (10): 2658-2668 (2018). PMID 30184500.
user report Mochizuki, K., Repression of Somatic Genes by Selective Recruitment of HDAC3 by BLIMP1 Is Essential for Mouse Primordial Germ Cell Fate Determination. Cell Rep. 24 (10): 2682-2693.e6 (2018). PMID 30184502.
user report Yanagihashi, Y., Mouse macrophages show different requirements for phosphatidylserine receptor Tim4 in efferocytosis. Proc. Natl. Acad. Sci. U.S.A. 114 (33): 8800-8805 (2017). PMID 28768810.
user report Nakamori, D., Direct conversion of human fibroblasts into hepatocyte-like cells by ATF5, PROX1, FOXA2, FOXA3, and HNF4A transduction. Sci. Rep. 7 (1): 16675 (2017). PMID 29192290.
user report Shcherbakova, D.M., Bright monomeric near-infrared fluorescent proteins as tags and biosensors for multiscale imaging. Nat. Commun. 7: 12405 (2016). PMID 27539380.
user report Matsu-Ura, T., Intercellular Coupling of the Cell Cycle and Circadian Clock in Adult Stem Cell Culture. Mol. Cell 64 (5): 900-912 (2016). PMID 27867006.
user report Naito, A., RFPL4A increases the G1 population and decreases sensitivity to chemotherapy in human colorectal cancer cells. J Biol Chem. 2015 Jan 20. pii: jbc.M114.614859 PMID 25605732.
user report Takahashi, H., Significance of Polypyrimidine Tract-Binding Protein 1 Expression in Colorectal Cancer. Mol. Cancer. Ther. 14 (7): 1705-1716 (2015). PMID 25904505.
user report Sato, Y., Disease modeling and lentiviral gene transfer in patient-specific induced pluripotent stem cells from late-onset Pompe disease patient. Mol. Ther. Methods Clin. Dev. 2: 15023 (2015). PMID 26199952.
user report Kim, Y.C., Androgen Receptor-Mediated Growth Suppression of HPr-1AR and PC3-Lenti-AR Prostate Epithelial Cells. PLoS One 10 (9): e0138286 (2015). PMID 26372468.
user report Morishima T., Genetic correction of HAX1 in induced pluripotent stem cells from a patient with severe congenital neutropenia improves defective granulopoiesis. Haematologica 99 (1): 19-27 (2014). PMID 23975175.
user report Matsumoto, S., A combination of Wnt and growth factor signaling induces Arl4c expression to form epithelial tubular structures. EMBO J., 33 (7): 702-718 (2014). PMID 24562386.
user report Maeda, S., Interferon-alpha Acts on the S/G2/M Phases to Induce Apoptosis in the G1 Phase of an IFNAR2-Expressing Hepatocellular Carcinoma Cell Line. J. Biol. Chem. 289 (34): 23786-23795 (2014). PMID 25012666.
user report Kojima, K., ESCRT-0 Protein Hepatocyte Growth Factor-regulated Tyrosine Kinase Substrate (Hrs) Is Targeted to Endosomes Independently of Signal-transducing Adaptor Molecule (STAM) and the Complex Formation with STAM Promotes Its Endosomal Dissociation. J. Biol. Chem. 289 (48): 33296-33310 (2014). PMID 25296754.
user report Kondo, S., An HLA-modified ovarian cancer cell line induced CTL responses specific to an epitope derived from claudin-1 presented by HLA-A*24:02 molecules. Hum. Immunol., 74 (9): 1103-1110 (2013). PMID 23806269.
user report Inaguma, S., GLI1 Interferes with the DNA Mismatch Repair System in Pancreatic Cancer through BHLHE41-Mediated Suppression of MLH1. Cancer Res., 73 (24): 7313-7323 (2013). PMID 24165159.
user report Kagawa, Y., Cell Cycle-Dependent Rho GTPase Activity Dynamically Regulates Cancer Cell Motility and Invasion In Vivo. PLoS One, 8 (12): e83629 (2013). PMID 24386239.
user report Toyomitsu, E., CCL2 promotes P2X4 receptor trafficking to the cell surface of microglia. Purinerg. Signal, 8 (2): 301-310 (2012). PMID 22222817.
user report Hirota, Y., Roles of planar cell polarity signaling in maturation of neuronal precursor cells in the postnatal mouse olfactory bulb. Stem Cells, 30 (8): 1726-1733 (2012). PMID 22628050.
user report Masuda, T., IRF8 Is a Critical Transcription Factor for Transforming Microglia into a Reactive Phenotype. Cell Rep., 1 (4): 334-340 (2012). PMID 22832225.
user report Sakaue-Sawano, A., Drug-induced cell cycle modulation leading to cell-cycle arrest, nuclear mis-segregation, or endoreplication. BMC Cell Biol. 12: 2 (2011). PMID 21226962.
user report Amako, Y., Protein kinase D negatively regulates hepatitis C virus secretion through phosphorylation of oxysterol-binding protein and ceramide transfer protein. J. Biol. Chem., 286 (13): 11265-11274 (2011). PMID 21285358.
user report Ibi, M., Trichoplein controls microtubule anchoring at the centrosome by binding to Odf2 and ninein. J. Cell Sci., 124 (Pt 6): 857-864 (2011). PMID 21325031.
user report Hirota Y., Planar polarity of multiciliated ependymal cells involves the anterior migration of basal bodies regulated by non-muscle myosin II. Development, 137, 3037-3046 (2010). PMID 20685736.
user report Sasai, M., Bifurcation of Toll-like receptor 9 signaling by adaptor protein 3. Science 329 (5998): 1530-1534 (2010). PMID 20847273.
user report Kiriyama, M., Interaction of FLASH with arsenite resistance protein 2 is involved in cell cycle progression at S phase. Mol. Cell. Biol., 29, 4729-4741 (2009). PMID 19546234.
user report Shinoda Y., Efficient transduction of cytotoxic and anti-HIV-1 genes by a gene-regulatable lentiviral vector. Virus Genes., 39, 165-175 (2009). PMID 19554442.
user report Isogawa, M., Rearranged NF-kappa B2 gene in an adult T-cell leukemia cell line. Cancer Sci., 99 (4): 792-798 (2008). PMID 18377428.
user report Higuchi, M., Cooperation of NF-kappaB2/p100 activation and the PDZ domain binding motif signal in human T-cell leukemia virus type 1 (HTLV-1) Tax1 but not HTLV-2 Tax2 is crucial for interleukin-2-independent growth transformation of a T-cell line. J. Virol. 81 (21): 11900-11907 (2007). PMID 17715223.

2020.12.30

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