Resource data sheet
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CSII-EF-MCS

Backbone vector of lentivirus construct with EF-1 alpha promoter.

Catalog number RDB04378
Resource name CSII-EF-MCS
Alternative name pCSII-EF-MCS
Clone info. Backbone vector of lentivirus construct with EF-1 alpha promoter.
Commonly requested with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Vector backbone Plasmid
Selectable markers Amp^r (E. coli); Zeo^r (plasmid transfection of mammalian cells).
Originator Miyoshi, Hiroyuki |
CLICK here for plasmid list. [link]

Distribution information

Terms and conditions for distribution The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe Dr. Hiroyuki Miyoshi (The Developer, the current address: Keio University) as the Developer. Dr. Miyoshi may request the RECIPIENT to report on progress and/or results obtained through the use of the BIOLOGICAL RESOURCE.
Ordering Please visit Ordering instruction. 
Order form [Credit Card Payment]  [Bank Transfer or Check Payment]
Material Transfer Agreement (MTA) [Word]
Remarks ((Remarks)) Remember that you will be working with samples containing infectious virus.
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB04378 CSII-EF-MCS DNA solution

Ordering instruction of plasmids [in Japanese] [in English]

References and tips

Electronic file

Electronic file Sequence RDB04378z.seq from Depositor
Electronic file PDF RDB04378.pdf from Depositor

Featured content

References

original Ueda, N., Generation of TCR-Expressing Innate Lymphoid-like Helper Cells that Induce Cytotoxic T Cell-Mediated Anti-leukemic Cell Response. Stem Cell Reports 10 (6): 1935-1946 (2018). PMID 29805109.

User reports and related articles (go to bottom)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB12259_A5Kpp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxEF_F2 RDB12259_A5Kpa.seq
>04378_12259_A5Kp_pAxEF_F2_E10_13.ab1
    1 TTTTTCATTT CAGTGTCGTG ACACGCTACC GGTCTCGAGA ATTCTGCAGC GGCCGCGGAT
   61 CCTCTAGAGT TAACATCGAG GGATCAAGCT TATCGATAAT CAACCTCTGG ATTACAAAAT
  121 TTGTGAAAGA TTGACTGGTA TTCTTAACTA TGTTGCTCCT TTTACGCTAT GTGGATACGC
  181 TGCTTTAATG CCTTTGTATC ATGCTATTGC TTCCCGTATG GCTTTCATTT TCTCCTCCTT
  241 GTATAAATCC TGGTTGCTGT CTCTTTATGA GGAGTTGTGG CCCGTTGTCA GGCAACGTGG
  301 CGTGGTGTGC ACTGTGTTTG CTGACGCAAC CCCCACTGGT TGGGGCATTG CCACCACCTG
  361 TCAGCTCCTT TCCGGGACTT TCGCTTTCCC CCTCCCTATT GCCACGGCGG AACTCATCGC
  421 CGCCTGCCTT GCCCGCTGCT GGACAGGGGC TCGGCTGTTG GGCACTGACA ATTCCGTGGT
  481 GTTGTCGGGG AAGCTGACGT CCTTTCCATG GCTGCTCGCC TGTGTTGCCA CCTGGATTCT
  541 GCGCGGGACG TCCTTCTGCT ACGTCCCTTC GGCCCTCAAT CCAGCGGACC TTCCTTCCCG
  601 CGGCCTGCTG CCGGCTCTGC GGCCTCTTCC GCGTCTTCGC CTTCGCCCTC AGACGAGTCG
  661 GATCTCCCTT TGGGCCGCCT CCCCGCATCG ATACCGTCGA GACCTAGAAA AACATGGAGC
  721 AATCACAAGT AGCAATACAG CAGCTACCAA TGCTGATTGT GCCTGGCTAG AAGCACAAGA
  781 GGAGGAGGAG GTGGGTTTTC CAGTCACACC TCAGGTACCT TTAAGACCAA TGACTTACAA
  841 GGCAGCTGTA GATCTTAGCC ACTTTTTAAA AGAAAAGGGG GGACTGGAAG GGCTAATTCA
  901 CTCCCAACGA AGACAAGATA TCCTTGATCT GTGGATCTAC CACACACAAA GGCTACTTCC
  961 CTGATTGGCA GAACTACACA CCAGGGCCCA GGGATCAGAT ATCCACTGAC CCTTTGGGAT
 1021 GGTGCTACAA GCTAGTACCA GTTTGAGCAA GAGACGTAGA GAGCCAATGA AGGAGAGAAC
 1081 ACCCGCTGTA CACCCTGTGA GCCCTGCCAT GGGAATGAAT GAACCGGAGA AGAGAAGTAT
1141 TAGAAGGTTG GAAGGTTTGA ACAGAG
//
Primer: CMV-Forward RDB12259_A5Kpb.seq
>04378_12259_A5Kp_CMV-Forward_F10_16.ab1
    1 CGTTCACCTC GGCCCATTGA CGCAATGGGC GGTAGGCGTG TACGGTGGGA GGTCTATATA
   61 AGCAGCGCGT TTTGCCTGTA CTGGGTCTCT CTGGTTAGAC CAGATCTGAG CCTGGGAGCT
  121 CTCTGGCTAA CTAGGGAACC CACTGCTTAA GCCTCAATAA AGCTTGCCTT GAGTGCTTCA
  181 AGTAGTGTGT GCCCGTCTGT TGTGTGACTC TGGTAACTAG AGATCCCTCA GACCCTTTTA
  241 GTCAGTGTGG AAAATCTCTA GCAGTGGCGC CCGAACAGGG ACTTGAAAGC GAAAGGGAAA
  301 CCAGAGGAGC TCTCTCGACG CAGGACTCGG CTTGCTGAAG CGCGCACGGC AAGAGGCGAG
  361 GGGCGGCGAC TGGTGAGTAC GCCAAAAATT TTGACTAGCG GAGGCTAGAA GGAGAGAGAT
  421 GGGTGCGAGA GCGTCAGTAT TAAGCGGGGG AGAATTAGAT CGCGATGGGA AAAAATTCGG
  481 TTAAGGCCAG GGGGAAAGAA AAAATATAAA TTAAAACATA TAGTATGGGC AAGCAGGGAG
  541 CTAGAACGAT TCGCAGTTAA TCCTGGCCTG TTAGAAACAT CAGAAGGCTG TAGACAAATA
  601 CTGGGACAGC TACAACCATC CCTTCAGACA GGATCAGAAG AACTTAGATC ATTATATAAT
  661 ACAGTAGCAA CCCTCTATTG TGTGCATCAA AGGATAGAGA TAAAAGACAC CAAGGAAGCT
  721 TTAGACAAGA TAGAGGAAGA GCAAAACAAA AGTAAGACCA CCGCACAGCA AGGGCCGCTG
  781 ATCTTCAGAC CTGGAGGAGG AGATATGAGG GACAATTGGA GAAGTGAATT ATATAAATAT
  841 AAAGTAGTAA AAATTGAACC ATTAGGAGTA GCACCCACCA AGGCAAAGAG AAGAGTGGTG
  901 CAGAGAGAAA AAAGAGCAGT GGGAATAGGA GCTTTGTTCC TTGGGTTCTT GGGAGCAGCA
  961 GGAAGCACTA TGGGCGCAGC GTCAATGACG CTGACGGTAC AGGCCAGACA ATTTATTTGT
 1021 CTGGTATAGT GCAGCAGCAG AACAATTTGC TGAGGGCTAT TGGAGGCGCA CAGCATCTGC
 1081 TGCAACTTCA CAGGTCTGGG GCATCAGGCC AGGCTCAGGC AGAAATCCCT GACCTGGTGT
1141 GG
//
Primer: BGH_rev RDB12259_A5Kpc.seq
>04378_12259_A5Kp_BGHrev_G10_19.ab1
    1 GTTCGGGGGA CTGCTAGAGA TTTTCCACAC TGACTAAAAG GGTCTGAGGG ATCTCTAGTT
   61 ACCAGAGTCA CACAACAGAC GGGCACACAC TACTTGAAGC ACTCAAGGCA AGCTTTATTG
  121 AGGCTTAAGC AGTGGGTTCC CTAGTTAGCC AGAGAGCTCC CAGGCTCAGA TCTGGTCTAA
  181 CCAGAGAGAC CCAGTACAGT CCGGATGCAG CTCTCGGGCC ATGTGATGAA ATGCTAGGCG
  241 GCTGTCAAAC CTCCACTCTA ATACTTCTCT CTCCGGGTCA TCCATCCCAT GCAGGCTCAC
  301 AGGGTGTAAC AAGCGGGTGT TCTCTCCTTC ATTGGCTTCT TCTACCTTCT CTTGCTCAAC
  361 TGGTACTAGC TTGTAGCACC ATCCAAAGGT CAGTGGATAT CTGATCCCTG GCCCTGGTGT
  421 GTAGTTCTGC CAATCAGGGA AGTAGCCTTG TGTGTGGTAG ATCCACAGAT CAAGGATATC
  481 TTGTCTTCGT TGGGAGTGAA TTAGCCCTTC CAGTCCCCCC TTTTCTTTTA AAAAGTGGCT
  541 AAGATCTACA GCTGCCTTGT AAGTCATTGG TCTTAAAGGT ACCTGAGGTG TGACTGGAAA
  601 ACCCACCTCC TCCTCCTCTT GTGCTTCTAG CCAGGCACAA TCAGCATTGG TAGCTGCTGT
  661 ATTGCTACTT GTGATTGCTC CATGTTTTTC TAGGTCTCGA CGGTATCGAT GCGGGGAGGC
  721 GGCCCAAAGG GAGATCCGAC TCGTCTGAGG GCGAAGGCGA AGACGCGGAA GAGGCCGCAG
  781 AGCCGGCAGC AGGCCGCGGG AAGGAAGGTC CGCTGGATTG AGGGCCGAAG GGACGTAGCA
  841 GAAGGACGTC CCGCGCAGAA TCCAGGTGGC AACACAGGCG AGCAGCCATG GAAAGGACGT
  901 CAGCTTCCCC GACAACACCA CGGAATTGTC AGTGCCCAAC AGCCGAGCCC CTGTCCAGCA
  961 GCGGGCAAGG CAGGCGGCGA TGAGTTCCCG CCGTGGCAAT AGGGAGGGGG AAAGCGAAAG
 1021 TCCCGGGAAG GAGCTGACAG TGGTGGCATG CCCCAACAGT GGGGTGCGTC AGCAACACAG
 1081 TGCACACCAC GCCACGTGCT GACACGGGCC ACGACTCCTC ATAAGAGACA GCAACAGCAT
1141 TTATACACGG AGGAAGAAAA ATGGAAAGGC CTACG
//
Primer: SV40pro_F RDB12259_A5Kpd.seq
>04378_12259_A5Kp_SV40prp_F_H10_22.ab1
    1 CCTTGTCCTG GCATTCAGAG TAGTGAGGAG GCTTTTTTGG AGGCCTAGGC TTTTGCAAAA
   61 AGCTCCCGGG AGCTTGTATA TCCATTTTCG GATCTGATCA GCACGTGTTG ACAATTAATC
  121 ATCGGCATAG TATATCGGCA TAGTATAATA CGACAAGGTG AGGAACTAAA CCATGGCCAA
  181 GTTGACCAGT GCCGTTCCGG TGCTCACCGC GCGCGACGTC GCCGGAGCGG TCGAGTTCTG
  241 GACCGACCGG CTCGGGTTCT CCCGGGACTT CGTGGAGGAC GACTTCGCCG GTGTGGTCCG
  301 GGACGACGTG ACCCTGTTCA TCAGCGCGGT CCAGGACCAG GTGGTGCCGG ACAACACCCT
  361 GGCCTGGGTG TGGGTGCGCG GCCTGGACGA GCTGTACGCC GAGTGGTCGG AGGTCGTGTC
  421 CACGAACTTC CGGGACGCCT CCGGGCCGGC CATGACCGAG ATCGGCGAGC AGCCGTGGGG
  481 GCGGGAGTTC GCCCTGCGCG ACCCGGCCGG CAACTGCGTG CACTTCGTGG CCGAGGAGCA
  541 GGACTGACAC GTGCTACGAG ATTTCGATTC CACCGCCGCC TTCTATGAAA GGTTGGGCTT
  601 CGGAATCGTT TTCCGGGACG CCGGCTGGAT GATCCTCCAG CGCGGGGATC TCATGCTGGA
  661 GTTCTTCGCC CACCCCAACT TGTTTATTGC AGCTTATAAT GGTTACAAAT AAAGCAATAG
  721 CATCACAAAT TTCACAAATA AAGCATTTTT TTCACTGCAT TCTAGTTGTG GTTTGTCCAA
  781 ACTCATCAAT GTATCTTATC ATGTCTGTAT ACCGTCGACC TCTAGCTAGA GCTTGGCGTA
  841 ATCATGGTCA TAGCTGTTTC CTGTGTGAAA TTGTTATCCG CTCACAATTC CACACAACAT
  901 ACGAGCCGGA AGCATAAAGT GTAAAGCCTG GGGTGCCTAA TGAGTGAGCT AACTCACATT
  961 AATTGCGTTG CGCTCACTGC CCGCTTTCCA GTCGGGAAAC CTGTCGTGCC AGCTGCATTA
 1021 ATGAATCGGG CCAACGCGCG GGGAGAGCGG TTGCGTATTG GCGCTCTTCG CCTTCTCGCT
 1081 CACTGACTCG CTGCGCTCGG TCGTCGGCTG CGCCGAGCGG TATCAGCTCA CCTCAAAGGC
1141 CGGTTAAATA ACC
//

Please visit Sequencing and PCR primers for primer information.

References

References

original Ueda, N., Generation of TCR-Expressing Innate Lymphoid-like Helper Cells that Induce Cytotoxic T Cell-Mediated Anti-leukemic Cell Response. Stem Cell Reports 10 (6): 1935-1946 (2018). PMID 29805109.
user report Nakashima, M., Trogocytosis of ligand-receptor complex and its intracellular transport in CD30 signalling. Biol Cell. 2018 Feb 12. doi: 10.1111/boc.201800002. [Epub ahead of print] PMID 29431186.
user report Chiyonobu, N., Fatty Acid Binding Protein 4 (FABP4) Overexpression in Intratumoral Hepatic Stellate Cells within Hepatocellular Carcinoma with Metabolic Risk Factors. Am. J. Pathol. 188 (5): 1213-1224 (2018). PMID 29454748.
user report Kasahara, K., EGF receptor kinase suppresses ciliogenesis through activation of USP8 deubiquitinase. Nat. Commun. 9 (1): 758 (2018). PMID 29472535.
user report Mochizuki, K., Repression of Somatic Genes by Selective Recruitment of HDAC3 by BLIMP1 Is Essential for Mouse Primordial Germ Cell Fate Determination. Cell Rep. 24 (10): 2682-2693.e6 (2018). PMID 30184502.
user report Yanagihashi, Y., Mouse macrophages show different requirements for phosphatidylserine receptor Tim4 in efferocytosis. Proc. Natl. Acad. Sci. U.S.A. 114 (33): 8800-8805 (2017). PMID 28768810.
user report Nakamori, D., Direct conversion of human fibroblasts into hepatocyte-like cells by ATF5, PROX1, FOXA2, FOXA3, and HNF4A transduction. Sci. Rep. 7 (1): 16675 (2017). PMID 29192290.
user report Shcherbakova, D.M., Bright monomeric near-infrared fluorescent proteins as tags and biosensors for multiscale imaging. Nat. Commun. 7: 12405 (2016). PMID 27539380.
user report Matsu-Ura, T., Intercellular Coupling of the Cell Cycle and Circadian Clock in Adult Stem Cell Culture. Mol. Cell 64 (5): 900-912 (2016). PMID 27867006.
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user report Sato, Y., Disease modeling and lentiviral gene transfer in patient-specific induced pluripotent stem cells from late-onset Pompe disease patient. Mol. Ther. Methods Clin. Dev. 2: 15023 (2015). PMID 26199952.
user report Kim, Y.C., Androgen Receptor-Mediated Growth Suppression of HPr-1AR and PC3-Lenti-AR Prostate Epithelial Cells. PLoS One 10 (9): e0138286 (2015). PMID 26372468.
user report Morishima T., Genetic correction of HAX1 in induced pluripotent stem cells from a patient with severe congenital neutropenia improves defective granulopoiesis. Haematologica 99 (1): 19-27 (2014). PMID 23975175.
user report Matsumoto, S., A combination of Wnt and growth factor signaling induces Arl4c expression to form epithelial tubular structures. EMBO J., 33 (7): 702-718 (2014). PMID 24562386.
user report Maeda, S., Interferon-alpha Acts on the S/G2/M Phases to Induce Apoptosis in the G1 Phase of an IFNAR2-Expressing Hepatocellular Carcinoma Cell Line. J. Biol. Chem. 289 (34): 23786-23795 (2014). PMID 25012666.
user report Kojima, K., ESCRT-0 Protein Hepatocyte Growth Factor-regulated Tyrosine Kinase Substrate (Hrs) Is Targeted to Endosomes Independently of Signal-transducing Adaptor Molecule (STAM) and the Complex Formation with STAM Promotes Its Endosomal Dissociation. J. Biol. Chem. 289 (48): 33296-33310 (2014). PMID 25296754.
user report Kondo, S., An HLA-modified ovarian cancer cell line induced CTL responses specific to an epitope derived from claudin-1 presented by HLA-A*24:02 molecules. Hum. Immunol., 74 (9): 1103-1110 (2013). PMID 23806269.
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2019.03.08

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