Resource data sheet
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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

CSII-CMV-MCS

Backbone vector of lentivirus construct with CMV promoter.

Catalog number RDB04377
Resource name CSII-CMV-MCS
Alternative name pCSII-CMV-MCS
Clone info. Backbone vector of lentivirus construct with CMV promoter.
Commonly requested with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Vector backbone Plasmid
Selectable markers Please note that Zeo resistance marker is not included in the lentivirus produced from this vector. E. coli: Amp^r.
Originator Miyoshi, Hiroyuki |
Lentiviral plasmid list, maps and sequences information. [link]

Distribution information

Terms and conditions for distribution The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe Dr. Hiroyuki Miyoshi as the Developer.
Ordering Please visit Ordering instruction. 
Order form 
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Material Transfer Agreement (MTA) [Word]
Remarks Remember that you will be working with samples containing infectious virus.
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB04377 CSII-CMV-MCS DNA solution

Ordering instruction of plasmids [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Electronic file Sequence RDB04377z.seq
Electronic file Remarks, protocol and/or map (pdf) RDB04377.pdf

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content


Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB17907_B0Aep1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: cPPT+CTS_F
Region: CMV en2, CMV pro2, MCS, PRE
Sequence file: RDB17907_B0Aea.seq check
>04377_17907_B0Ae_1_cPPT+CTS_F_A07_01_ABI24.ab1
    1 NNNNNNNNNN NNNNTCGNNT AATAGTAANN NATTACGGGG TCATTAGTTC NTAGCCCATA
   61 TATGGAGTTC CGCGTTACAT AACTTACGGT AAATGGCCCG CCTGGCTGAC CGCCCAACGA
  121 CCCCCGCCCA TTGACGTCAA TAATGACGTA TGTTCCCATA GTAACGCCAA TAGGGACTTT
  181 CCATTGACGT CAATGGGTGG AGTATTTACG GTAAACTGCC CACTTGGCAG TACATCAAGT
  241 GTATCATATG CCAAGTACGC CCCCTATTGA CGTCAATGAC GGTAAATGGC CCGCCTGGCA
  301 TTATGCCCAG TACATGACCT TATGGGACTT TCCTACTTGG CAGTACATCT ACGTATTAGT
  361 CATCGCTATT ACCATGGTGA TGCGGTTTTG GCAGTACATC AATGGGCGTG GATAGCGGTT
  421 TGACTCACGG GGATTTCCAA GTCTCCACCC CATTGACGTC AATGGGAGTT TGTTTTGGCA
  481 CCAAAATCAA CGGGACTTTC CAAAATGTCG TAACAACTCC GCCCCATTGA CGCAAATGGG
  541 CGGTAGGCGT GTACGGTGGG AGGTCTATAT AAGCAGAGCT GGTTTAGTGA ACCGTCAGAT
  601 CCGCTAGCGC TACCGGTCTC GAGAATTCTG CAGCGGCCGC GGATCCTCTA GAGTTAACAT
  661 CGAGGGATCA AGCTTATCGA TAATCAACCT CTGGANTACA AAATTTGTGA AAGANTGNCT
  721 GGTATTCTTA ACTATGTTGC TCCTTTTACG CTATGTGGAT ACGCTGCTTT AATGCCTTTG
  781 TATCATGCTA TTGCTTCCCG TATGGCTTTC ATTTTCTCCT CCTTGTATAA ATCCTGGTTG
  841 CTGTCTCNTT ATGANGAGTT GTGGCCCNTT NNCANGNAAC GNNNCNNNNN NNGCNNNNNN
901 NTNGCNNANN CNACCCCCAC NNGNNNGGGG NNTTGCCNNN NNCNNNNNNN NNN
//
Primer: HIV-B_R
Region: HIV-1 psi, 5'LTR, CMV pro1, CMV en1
Sequence file: RDB17907_B0Aeb.seq check
>04377_17907_B0Ae_1_HIV-B_R_C07_07_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NTTTTGGCGT ANTCNCCNGT CGCCGCCCCT CGCCTCTTGC
   61 CGTGCGCGCT TCAGCAAGCC GAGTCCTGCG TCGAGAGAGC TCCTCTGGTT TCCCTTTCGC
  121 TTTCAAGTCC CTGTTCGGGC GCCACTGCTA GAGATTTTCC ACACTGACTA AAAGGGTCTG
  181 AGGGATCTCT AGTTACCAGA GTCACACAAC AGACGGGCAC ACACTACTTG AAGCACTCAA
  241 GGCAAGCTTT ATTGAGGCTT AAGCAGTGGG TTCCCTAGTT AGCCAGAGAG CTCCCAGGCT
  301 CAGATCTGGT CTAACCAGAG AGACCCAGTA CAGGCAAAAC GCGCTGCTTA TATAGACCTC
  361 CCACCGTACA CGCCTACCGC CCATTTGCGT CAATGGGGCG GAGTTGTTAC GACATTTTGG
  421 AAAGTCCCGT TGATTTTGGT GCCAAAACAA ACTCCCATTG ACGTCAATGG GGTGGAGACT
  481 TGGAAATCCC CGTGAGTCAA ACCGCTATCC ACGCCCATTG ATGTACTGCC AAAACCGCAT
  541 CACCATGGTA ATAGCGATGA CTAATACGTA GATGTACTGC CAAGTAGGAA AGTCCCATAA
  601 GGTCATGTAC TGGGCATAAT GCCAGGCGGG CCATTTACCG TCATTGACGT CAATAGGGGG
  661 CGTACTTGGC ATATGATACA CTTGATGTAC TGCCAAGTGG GCAGTTTACC GTAAATACTC
  721 CACCCATTGA CGTCAATGGA AAGTCCCTAT TGNCGTTACT ATGGGAACAT ACGTCATTAT
  781 TGACGTCNAT GNGCGGGGGT CGTTGGGCGG NCAGCCAGGC GGGCNATTTA CNGNAAGTTA
  841 TGTAACGCGG AACTCNNTAT ATGGGCTATG ANCNNANGAN CNCNNNAATT NNNNACTATT
901 AATAANNANT CNANAATCAA NGTCNNNNNN NATATCNNGN CNNNNNNNNN N
//
Primer: RRE_F1
Region: RRE, cPPT_CTS
Sequence file: RDB17907_B0Aec.seq check
>04377_17907_B0Ae_1_RRE_F1_D07_10_ABI24.ab1
    1 NNNNNNNNNN NNNCNNCNNN NNNNTGCTGN NGGCTATTGA GGCGCAACAG CATCTGTTGC
   61 AACTCACAGT CTGGGGCATC AAGCAGCTCC AGGCAAGAAT CCTGGCTGTG GAAAGATACC
  121 TAAAGGATCA ACAGCTCCTG GGGATTTGGG GTTGCTCTGG AAAACTCATT TGCACCACTG
  181 CTGTGCCTTG GAATGCTAGT TGGAGTAATA AATCTCTGGA ACAGATTTGG AATCACACGA
  241 CCTGGATGGA GTGGGACAGA GAAATTAACA ATTACACAAG CTTAATACAC TCCTTAATTG
  301 AAGAATCGCA AAACCAGCAA GAAAAGAATG AACAAGAATT ATTGGAATTA GATAAATGGG
  361 CAAGTTTGTG GAATTGGTTT AACATAACAA ATTGGCTGTG GTATATAAAA TTATTCATAA
  421 TGATAGTAGG AGGCTTGGTA GGTTTAAGAA TAGTTTTTGC TGTACTTTCT ATAGTGAATA
  481 GAGTTAGGCA GGGATATTCA CCATTATCGT TTCAGACCCA CCTCCCAACC CCGAGGGGAC
  541 CCGACAGGCC CGAAGGAATA GAAGAAGAAG GTGGAGAGAG AGACAGAGAC AGATCCATTC
  601 GATTAGTGAA CGGATCTACA AATGGCAGTA TTCATCCACA ATTTTAAAAG AAAAGGGGGG
  661 ANTGGGGGGT ACAGTGCAGG GGAAAGAATA GTAGACATAA TAGCAACAGA CATACAAACT
  721 AAAGAATTAC AAAAACNAAT TACAAAAATT CAAAATTTTC GGGTTTATTA CAGGNNCAGC
  781 AGAAATTCAC TTTGAATTAA TTCGATTAAT AGTAATCNAT TACNGGGNCA TTAGTTCATA
  841 GCCNNTATAT GGAGTTNCGC GNTACATAAC NTACNGNAAA TGGCCCGNNN NGNNNANNGC
  901 CCNACNACCC CCGCCCATNN NCNNNNNNNN NGNNNNANNN NCCNNTANNN ACNNNNNNNN
961 NN
//
Primer: BGH_rev2
Region: bGHp(A), 3'LTR,
Sequence file: RDB17907_B0Aed.seq check
>04377_17907_B0Ae_1_BGH_rev2_E07_13_ABI24.ab1
    1 NNNNNNNNNN NNNNNNGGGG NNGGNNNNNN ACAGATGGCT GGCAACTAGA AGGCACAGTC
   61 GAGGCTGATC AGCGGGTTTA AACGGGCCCT GCTAGAGATT TTCCACACTG ACTAAAAGGG
  121 TCTGAGGGAT CTCTAGTTAC CAGAGTCACA CAACAGACGG GCACACACTA CTTGAAGCAC
  181 TCAAGGCAAG CTTTATTGAG GCTTAAGCAG TGGGTTCCCT AGTTAGCCAG AGAGCTCCCA
  241 GGCTCAGATC TGGTCTAACC AGAGAGACCC AGTACAGTCC GGATGCAGCT CTCGGGCCAT
  301 GTGATGAAAT GCTAGGCGGC TGTCAAACCT CCACTCTAAT ACTTCTCTCT CCGGGTCATC
  361 CATCCCATGC AGGCTCACAG GGTGTAACAA GCGGGTGTTC TCTCCTTCAT TGGCTTCTTC
  421 TACCTTCTCT TGCTCAACTG GTACTAGCTT GTAGCACCAT CCAAAGGTCA GTGGATATCT
  481 GATCCCTGGC CCTGGTGTGT AGTTCTGCCA ATCAGGGAAG TAGCCTTGTG TGTGGTAGAT
  541 CCACAGATCA AGGATATCTT GTCTTCGTTG GGAGTGAATT AGCCCTTCCN GTCCCCNCTT
  601 TTCTTTTAAA AAGTGGCTAA GATCTACAGC TGCCTTGTAA GTCATTGGTC TTAAAGGTAC
  661 CTGANGTGTG ACTGGAAAAC CCACCTCCTC CTCCTCTTGT GCTTCTAGCC AGGCACAATC
  721 AGCATTGGTA GCTGCTGTAT TGCTACTTGT GATTGNTNCN TGTTTTTCTA NGTCTCGANN
  781 GTATCGATGC NGGGNNGCNG CCCNAANGGA NNTNCNANTC GTCTNNNGNC GNANNNNAAN
841 ANNNNNNNNN N
//
Primer: SV40pro_ori_F
Region: SV40 pro_ori, EM7 pro, Zeo
Sequence file: RDB17907_B0Aee.seq check
>04377_17907_B0Ae_1_SV40pro_ori_F_F07_16_ABI24.ab1
    1 NNNNNNNNNN NNNNNGNNGC CTCTGCCTCT GNNCTATTCN NGAAGTAGTG AGGAGGCTTT
   61 TTTGGAGGCC TAGGCTTTTG CAAAAAGCTC CCGGGAGCTT GTATATCCAT TTTCGGATCT
  121 GATCAGCACG TGTTGACAAT TAATCATCGG CATAGTATAT CGGCATAGTA TAATACGACA
  181 AGGTGAGGAA CTAAACCATG GCCAAGTTGA CCAGTGCCGT TCCGGTGCTC ACCGCGCGCG
  241 ACGTCGCCGG AGCGGTCGAG TTCTGGACCG ACCGGCTCGG GTTCTCCCGG GACTTCGTGG
  301 AGGACGACTT CGCCGGTGTG GTCCGGGACG ACGTGACCCT GTTCATCAGC GCGGTCCAGG
  361 ACCAGGTGGT GCCGGACAAC ACCCTGGCCT GGGTGTGGGT GCGCGGCCTG GACGAGCTGT
  421 ACGCCGAGTG GTCGGAGGTC GTGTCCACGA ACTTCCGGGA CGCCTCCGGG CCGGCCATGA
  481 CCGAGATCGG CGAGCAGCCG TGGGGGCGGG AGTTCGCCCT GCGCGACCCG GCCGGCAACT
  541 GCGTGCACTT CGTGGCCGAG GAGCAGGACT GACACGTGCT ACGAGATTTC GATTCCACCG
  601 CCGCCTTCTA TGAAAGGTTG GGCTTCGGAA TCGTTTTCCG GGACGCCGGC TGGATGATCC
  661 TCCAGCGCGG GGNTCTCATG CTGGAGTTCT TCGCCCACCC CAACTTGTTT ATTGCAGCTT
  721 ATAATGGTTA CAAATAAAGC AATAGCATCA CAAATTTCAC AAATAAAGCA TTTTTTTCAC
  781 TGCATTCTAG TTGTGGTTTG TCCAAACTCN TCAANGTATC TTATCATGTC TGTATACNGT
  841 CNNNCTCTAG CTAGANCTTG GCGNAATCNT GGNCATANCT GTTNNCTGNG NGAAANNNNT
901 ATCCGCNNAC NATNCCNCAC NACNTACNAN NNNNNNNNN
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

reference Niwano, K., Lentiviral vector-mediated SERCA2 gene transfer protects against heart failure and left ventricular remodeling after myocardial infarction in rats. PMID 18388909.
user report Girao, H., CLASP2 binding to curved microtubule tips promotes flux and stabilizes kinetochore attachments. J. Cell Biol. 219 (2). pii: e201905080 (2020). PMID 31757788.
user report Ishikawa, T., Physiological role of urothelial cancer-associated one long noncoding RNA in human skeletogenic cell differentiation. J. Cell. Physiol. 233 (6): 4825-4840 (2018). PMID 29150954.
user report Hamada, M., Integration Mapping of piggyBac-Mediated CD19 Chimeric Antigen Receptor T Cells Analyzed by Novel Tagmentation-Assisted PCR. EBioMedicine 34: 18-26 (2018). PMID 30082227.
user report Tanaka, M., Aggregation of scaffolding protein DISC1 dysregulates phosphodiesterase 4 in Huntington's disease. J. Clin. Invest. \127 (4): 1438-1450 (2017). PMID 28263187.
user report Ujino, S., Hepatitis C virus utilizes VLDLR as a novel entry pathway. Proc. Natl. Acad. Sci. U.S.A. 113 (1): 188-193 (2016). PMID 26699506.
user report Maki, T., CLASP2 Has Two Distinct TOG Domains That Contribute Differently to Microtubule Dynamics. J. Mol. Biol. 427 (14): 2379-2395 (2015). PMID 26003921.
user report Watanabe, K., FBXO21 mediates the ubiquitylation and proteasomal degradation of EID1. Genes Cells 20 (8): 667-674 (2015). PMID 26085330.
user report Uemura, S., Expression machinery of GM4: the excess amounts of GM3/GM4S synthase (ST3GAL5) are necessary for GM4 synthesis in mammalian cells. Glycoconj. J. 31 (2): 101-108 (2014). PMID 23999868.
user report Ohnishi, T., Identification and characterization of a neuron-specific isoform of protrudin. Genes Cells 19 (2): 97-111 (2014). PMID 24251978.
user report Araya, N., HTLV-1 induces a Th1-like state in CD4+CCR4+ T cells. J. Clin. Invest., 124 (8): 3431-42 (2014). PMID 24960164.
user report Grimaldi, A.D., CLASPs are required for proper microtubule localization of end-binding proteins. Dev. Cell 30 (3): 343-352 (2014). PMID 25117684.
user report Ishibashi, M., Clinical significance of the expression of long non-coding RNA HOTAIR in primary hepatocellular carcinoma. Oncol. Rep. 29 (3): 946-950 (2013). PMID 23292722.
user report Takahashi, Y., Paired related homoeobox 1, a new EMT inducer, is involved in metastasis and poor prognosis in colorectal cancer. Br. J. Cancer, 109 (2): 307-311 (2013). PMID 23807160.
user report Watanabe, T., FBL2 regulates amyloid precursor protein (APP) metabolism by promoting ubiquitination-dependent APP degradation and inhibition of APP endocytosis. J. Neurosci., 32 (10): 3352-3365 (2012). PMID 22399757.
user report Kanemura H., Impaired vascular development in the yolk sac and allantois in mice lacking RA-GEF-1. Biochem. Biophys. Res. Commun., 387, 754-759 (2009). PMID 19635461.
user report Sakurai, Y., DNA double strand break repair enzymes function at multiple steps in retroviral infection.
Retrovirology, 6:114 (2009). PMID 20003485.

2020.03.03

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