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Backbone vector plasmid of lentivirus construct with CMV promoter.
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External Database Reference sequence |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
| Terms and conditions set forth by the DEPOSITOR | The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer. |
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| Ordering | Please visit Ordering instruction.[link] Order form [Credit Card Payment ] [Bank Transfer Payment]Material Transfer Agreement (MTA, for use for not-for-profit academic purpose)[Word ] |
| Remarks | Remember that you will be working with samples containing infectious virus. |
[open/close]| 提供条件 | 本件研究材料は、非営利機関の非営利学術研究に限って提供する。本件研究材料を利用した研究結果等を発表する際は、本件研究材料が故三好浩之博士により開発されたことを明示する。 |
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| 提供依頼 | 手続きの詳細は、「レンチウイルスベクターの提供申し込み」をご覧ください。 提供依頼書 [Word ]提供同意書 (非営利機関による非営利学術研究用)[Word ]遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。 |
| 備考 | このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。 |
| Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
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| RDB04377 | CSII-CMV-MCS | DNA solution |
icon below as well as clone information before placing your order.Electronic file
| Sequence | RDB04377z.seq |
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| Remarks, protocol and/or map (pdf) | RDB04377.pdf |
Original reference
Further references such as user reports and related articles (go to bottom)
Featured content
| Featured content | Lentivirus Vector (English text) |
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| Featured content | Lentivirus Vector (Japanese text) |
RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.
| Test sheet | RDB17907_B0Aep1-1.pdf |
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Nucleotide sequence of a portion of this resource (if available).
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Primer: cPPT+CTS_F Region: CMV en2, CMV pro2, MCS, PRE Sequence file: RDB17907_B0Aea.seq
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>04377_17907_B0Ae_1_cPPT+CTS_F_A07_01_ABI24.ab1 1 NNNNNNNNNN NNNNTCGNNT AATAGTAANN NATTACGGGG TCATTAGTTC NTAGCCCATA 61 TATGGAGTTC CGCGTTACAT AACTTACGGT AAATGGCCCG CCTGGCTGAC CGCCCAACGA 121 CCCCCGCCCA TTGACGTCAA TAATGACGTA TGTTCCCATA GTAACGCCAA TAGGGACTTT 181 CCATTGACGT CAATGGGTGG AGTATTTACG GTAAACTGCC CACTTGGCAG TACATCAAGT 241 GTATCATATG CCAAGTACGC CCCCTATTGA CGTCAATGAC GGTAAATGGC CCGCCTGGCA 301 TTATGCCCAG TACATGACCT TATGGGACTT TCCTACTTGG CAGTACATCT ACGTATTAGT 361 CATCGCTATT ACCATGGTGA TGCGGTTTTG GCAGTACATC AATGGGCGTG GATAGCGGTT 421 TGACTCACGG GGATTTCCAA GTCTCCACCC CATTGACGTC AATGGGAGTT TGTTTTGGCA 481 CCAAAATCAA CGGGACTTTC CAAAATGTCG TAACAACTCC GCCCCATTGA CGCAAATGGG 541 CGGTAGGCGT GTACGGTGGG AGGTCTATAT AAGCAGAGCT GGTTTAGTGA ACCGTCAGAT 601 CCGCTAGCGC TACCGGTCTC GAGAATTCTG CAGCGGCCGC GGATCCTCTA GAGTTAACAT 661 CGAGGGATCA AGCTTATCGA TAATCAACCT CTGGANTACA AAATTTGTGA AAGANTGNCT 721 GGTATTCTTA ACTATGTTGC TCCTTTTACG CTATGTGGAT ACGCTGCTTT AATGCCTTTG 781 TATCATGCTA TTGCTTCCCG TATGGCTTTC ATTTTCTCCT CCTTGTATAA ATCCTGGTTG 841 CTGTCTCNTT ATGANGAGTT GTGGCCCNTT NNCANGNAAC GNNNCNNNNN NNGCNNNNNN 901 NTNGCNNANN CNACCCCCAC NNGNNNGGGG NNTTGCCNNN NNCNNNNNNN NNN // |
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Primer: HIV-B_R Region: HIV-1 psi, 5'LTR, CMV pro1, CMV en1 Sequence file: RDB17907_B0Aeb.seq
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>04377_17907_B0Ae_1_HIV-B_R_C07_07_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NTTTTGGCGT ANTCNCCNGT CGCCGCCCCT CGCCTCTTGC 61 CGTGCGCGCT TCAGCAAGCC GAGTCCTGCG TCGAGAGAGC TCCTCTGGTT TCCCTTTCGC 121 TTTCAAGTCC CTGTTCGGGC GCCACTGCTA GAGATTTTCC ACACTGACTA AAAGGGTCTG 181 AGGGATCTCT AGTTACCAGA GTCACACAAC AGACGGGCAC ACACTACTTG AAGCACTCAA 241 GGCAAGCTTT ATTGAGGCTT AAGCAGTGGG TTCCCTAGTT AGCCAGAGAG CTCCCAGGCT 301 CAGATCTGGT CTAACCAGAG AGACCCAGTA CAGGCAAAAC GCGCTGCTTA TATAGACCTC 361 CCACCGTACA CGCCTACCGC CCATTTGCGT CAATGGGGCG GAGTTGTTAC GACATTTTGG 421 AAAGTCCCGT TGATTTTGGT GCCAAAACAA ACTCCCATTG ACGTCAATGG GGTGGAGACT 481 TGGAAATCCC CGTGAGTCAA ACCGCTATCC ACGCCCATTG ATGTACTGCC AAAACCGCAT 541 CACCATGGTA ATAGCGATGA CTAATACGTA GATGTACTGC CAAGTAGGAA AGTCCCATAA 601 GGTCATGTAC TGGGCATAAT GCCAGGCGGG CCATTTACCG TCATTGACGT CAATAGGGGG 661 CGTACTTGGC ATATGATACA CTTGATGTAC TGCCAAGTGG GCAGTTTACC GTAAATACTC 721 CACCCATTGA CGTCAATGGA AAGTCCCTAT TGNCGTTACT ATGGGAACAT ACGTCATTAT 781 TGACGTCNAT GNGCGGGGGT CGTTGGGCGG NCAGCCAGGC GGGCNATTTA CNGNAAGTTA 841 TGTAACGCGG AACTCNNTAT ATGGGCTATG ANCNNANGAN CNCNNNAATT NNNNACTATT 901 AATAANNANT CNANAATCAA NGTCNNNNNN NATATCNNGN CNNNNNNNNN N // |
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Primer: RRE_F1 Region: RRE, cPPT_CTS Sequence file: RDB17907_B0Aec.seq
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>04377_17907_B0Ae_1_RRE_F1_D07_10_ABI24.ab1 1 NNNNNNNNNN NNNCNNCNNN NNNNTGCTGN NGGCTATTGA GGCGCAACAG CATCTGTTGC 61 AACTCACAGT CTGGGGCATC AAGCAGCTCC AGGCAAGAAT CCTGGCTGTG GAAAGATACC 121 TAAAGGATCA ACAGCTCCTG GGGATTTGGG GTTGCTCTGG AAAACTCATT TGCACCACTG 181 CTGTGCCTTG GAATGCTAGT TGGAGTAATA AATCTCTGGA ACAGATTTGG AATCACACGA 241 CCTGGATGGA GTGGGACAGA GAAATTAACA ATTACACAAG CTTAATACAC TCCTTAATTG 301 AAGAATCGCA AAACCAGCAA GAAAAGAATG AACAAGAATT ATTGGAATTA GATAAATGGG 361 CAAGTTTGTG GAATTGGTTT AACATAACAA ATTGGCTGTG GTATATAAAA TTATTCATAA 421 TGATAGTAGG AGGCTTGGTA GGTTTAAGAA TAGTTTTTGC TGTACTTTCT ATAGTGAATA 481 GAGTTAGGCA GGGATATTCA CCATTATCGT TTCAGACCCA CCTCCCAACC CCGAGGGGAC 541 CCGACAGGCC CGAAGGAATA GAAGAAGAAG GTGGAGAGAG AGACAGAGAC AGATCCATTC 601 GATTAGTGAA CGGATCTACA AATGGCAGTA TTCATCCACA ATTTTAAAAG AAAAGGGGGG 661 ANTGGGGGGT ACAGTGCAGG GGAAAGAATA GTAGACATAA TAGCAACAGA CATACAAACT 721 AAAGAATTAC AAAAACNAAT TACAAAAATT CAAAATTTTC GGGTTTATTA CAGGNNCAGC 781 AGAAATTCAC TTTGAATTAA TTCGATTAAT AGTAATCNAT TACNGGGNCA TTAGTTCATA 841 GCCNNTATAT GGAGTTNCGC GNTACATAAC NTACNGNAAA TGGCCCGNNN NGNNNANNGC 901 CCNACNACCC CCGCCCATNN NCNNNNNNNN NGNNNNANNN NCCNNTANNN ACNNNNNNNN 961 NN // |
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Primer: BGH_rev2 Region: bGHp(A), 3'LTR, Sequence file: RDB17907_B0Aed.seq
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>04377_17907_B0Ae_1_BGH_rev2_E07_13_ABI24.ab1 1 NNNNNNNNNN NNNNNNGGGG NNGGNNNNNN ACAGATGGCT GGCAACTAGA AGGCACAGTC 61 GAGGCTGATC AGCGGGTTTA AACGGGCCCT GCTAGAGATT TTCCACACTG ACTAAAAGGG 121 TCTGAGGGAT CTCTAGTTAC CAGAGTCACA CAACAGACGG GCACACACTA CTTGAAGCAC 181 TCAAGGCAAG CTTTATTGAG GCTTAAGCAG TGGGTTCCCT AGTTAGCCAG AGAGCTCCCA 241 GGCTCAGATC TGGTCTAACC AGAGAGACCC AGTACAGTCC GGATGCAGCT CTCGGGCCAT 301 GTGATGAAAT GCTAGGCGGC TGTCAAACCT CCACTCTAAT ACTTCTCTCT CCGGGTCATC 361 CATCCCATGC AGGCTCACAG GGTGTAACAA GCGGGTGTTC TCTCCTTCAT TGGCTTCTTC 421 TACCTTCTCT TGCTCAACTG GTACTAGCTT GTAGCACCAT CCAAAGGTCA GTGGATATCT 481 GATCCCTGGC CCTGGTGTGT AGTTCTGCCA ATCAGGGAAG TAGCCTTGTG TGTGGTAGAT 541 CCACAGATCA AGGATATCTT GTCTTCGTTG GGAGTGAATT AGCCCTTCCN GTCCCCNCTT 601 TTCTTTTAAA AAGTGGCTAA GATCTACAGC TGCCTTGTAA GTCATTGGTC TTAAAGGTAC 661 CTGANGTGTG ACTGGAAAAC CCACCTCCTC CTCCTCTTGT GCTTCTAGCC AGGCACAATC 721 AGCATTGGTA GCTGCTGTAT TGCTACTTGT GATTGNTNCN TGTTTTTCTA NGTCTCGANN 781 GTATCGATGC NGGGNNGCNG CCCNAANGGA NNTNCNANTC GTCTNNNGNC GNANNNNAAN 841 ANNNNNNNNN N // |
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Primer: SV40pro_ori_F Region: SV40 pro_ori, EM7 pro, Zeo Sequence file: RDB17907_B0Aee.seq
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>04377_17907_B0Ae_1_SV40pro_ori_F_F07_16_ABI24.ab1 1 NNNNNNNNNN NNNNNGNNGC CTCTGCCTCT GNNCTATTCN NGAAGTAGTG AGGAGGCTTT 61 TTTGGAGGCC TAGGCTTTTG CAAAAAGCTC CCGGGAGCTT GTATATCCAT TTTCGGATCT 121 GATCAGCACG TGTTGACAAT TAATCATCGG CATAGTATAT CGGCATAGTA TAATACGACA 181 AGGTGAGGAA CTAAACCATG GCCAAGTTGA CCAGTGCCGT TCCGGTGCTC ACCGCGCGCG 241 ACGTCGCCGG AGCGGTCGAG TTCTGGACCG ACCGGCTCGG GTTCTCCCGG GACTTCGTGG 301 AGGACGACTT CGCCGGTGTG GTCCGGGACG ACGTGACCCT GTTCATCAGC GCGGTCCAGG 361 ACCAGGTGGT GCCGGACAAC ACCCTGGCCT GGGTGTGGGT GCGCGGCCTG GACGAGCTGT 421 ACGCCGAGTG GTCGGAGGTC GTGTCCACGA ACTTCCGGGA CGCCTCCGGG CCGGCCATGA 481 CCGAGATCGG CGAGCAGCCG TGGGGGCGGG AGTTCGCCCT GCGCGACCCG GCCGGCAACT 541 GCGTGCACTT CGTGGCCGAG GAGCAGGACT GACACGTGCT ACGAGATTTC GATTCCACCG 601 CCGCCTTCTA TGAAAGGTTG GGCTTCGGAA TCGTTTTCCG GGACGCCGGC TGGATGATCC 661 TCCAGCGCGG GGNTCTCATG CTGGAGTTCT TCGCCCACCC CAACTTGTTT ATTGCAGCTT 721 ATAATGGTTA CAAATAAAGC AATAGCATCA CAAATTTCAC AAATAAAGCA TTTTTTTCAC 781 TGCATTCTAG TTGTGGTTTG TCCAAACTCN TCAANGTATC TTATCATGTC TGTATACNGT 841 CNNNCTCTAG CTAGANCTTG GCGNAATCNT GGNCATANCT GTTNNCTGNG NGAAANNNNT 901 ATCCGCNNAC NATNCCNCAC NACNTACNAN NNNNNNNNN // |
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Sequencing and PCR primers for primer information.
Original, user report and related articles
2022.05.18
GNP_filter3_RDBDEP_html_220516.pl