CSII-CMV-MCS (#RDB04377)
Backbone vector plasmid of lentivirus construct with CMV promoter.
 Drawn by SnapGene® software |
|
| Alternative name | pCSII-CMV-MCS |
|---|---|
| Clone info. | Backbone vector plasmid of lentivirus construct with CMV promoter. |
| Comment | Commonly requested with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394). |
| Vector backbone | Plasmid |
| Selectable markers | Ampicillin (E. coli). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector. |
| Growth conditions | 37C, LB+Amp |
| Depositor|Developer | Miyoshi, Hiroyuki | |
Distribution information
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
| Ordering forms | Order form [Credit Card Payment  ] [Bank Transfer Payment] [Example of order form ]MTA, for use for not-for-profit academic purpose [Word ] [Example of MTA ]Please visit Ordering instruction.[link] |
|---|---|
| Terms and conditions for distribution | The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer. |
| Remarks | Remember that you will be working with samples containing infectious virus. |
[open/close]| 必要書類 | 提供依頼書 [依頼書の記入例 ]提供同意書 (MTA, 非営利学術目的用)[Word ] [MTAの記入例 ]手続きの概要は、「レンチウイルスベクターの提供申し込み」をご覧ください。 |
|---|---|
| MTAに書く使用条件 | 本件研究材料は、非営利機関の非営利学術研究に限って提供する。本件研究材料を利用した研究結果等を発表する際は、本件研究材料が故三好浩之博士により開発されたことを明示する。 |
| 備考 | このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。 |
| Catalog # | Resource name | Shipping form | Fee |
|---|---|---|---|
| RDB04377 | CSII-CMV-MCS | DNA solution |
JPY 9,460 (not-for-profit academic purpose) plus cost of shipping containers, dry ice (if required) and shipping charge |
How to cite this biological resource
Materials & Methods section:
| The CSII-CMV-MCS was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB04377). |
Reference section:
Further references such as user reports and related articles (go to bottom)
References
Original, user report and related articles
| reference | Kuchimaru, T., A bright synthetic near-infrared luciferin enhances the capabilities of deep-tissue bioluminescence imaging using firefly luciferases. Research Square (2025). [Article] |
|---|---|
| reference | Garg, B., MICAL2 Is a Super Enhancer Associated Gene that Promotes Pancreatic Cancer Growth and Metastasis. bioRxiv doi: 10.1101/2024.06.26.600548 (2024). PMID 38979336. [PubMed] [Article] |
| reference | Niwano, K., Lentiviral vector-mediated SERCA2 gene transfer protects against heart failure and left ventricular remodeling after myocardial infarction in rats. Mol. Ther. 16 (6): 1026-1032 (2008). PMID 18388909. [PubMed] [Article] |
| user_report | Kimura, S., Combined Therapy Targeting MET and Pro-HGF Activation Shows Significant Therapeutic Effect Against Liver Metastasis of CRPC. Int. J. Mol. Sci. 26 (5): 2308 (2025). PMID 40076928. [PubMed] [Article] [RRC of NBRP] |
| user_report | Luo, W., CLASP2 recognizes tubulins exposed at the microtubule plus-end in a nucleotide state-sensitive manner. Sci. Adv. 9 (1): eabq5404 (2023). PMID 36598991. [PubMed] [Article] [RRC of NBRP] |
| user_report | Nakajima, M., Cofilin promotes vasculogenic mimicry by regulating the actin cytoskeleton in human breast cancer cells. FEBS Lett. 597 (8): 1114-1124 (2023). PMID 36737242. [PubMed] [Article] [RRC of NBRP] |
| user_report | Sato, M., Angiogenic inhibitor pre-administration improves the therapeutic effects of immunotherapy. Cancer Med. 12 (8): 9760-9773 (2023). PMID 36808261. [PubMed] [Article] [RRC of NBRP] |
| user_report | Fujinuma, S., FOXK1 promotes nonalcoholic fatty liver disease by mediating mTORC1-dependent inhibition of hepatic fatty acid oxidation. Cell Rep. 42 (5): 112530 (2023). PMID 37209098. [PubMed] [Article] [RRC of NBRP] |
| user_report | Minegishi, M., Secretory GFP reconstitution labeling of neighboring cells interrogates cell-cell interactions in metastatic niches. Nat. Commun. 14 (1): 8031 (2023). PMID 38052804. [PubMed] [Article] [RRC of NBRP] |
| user_report | Ueda, Y., Protocol for screening cellular outputs activated by optogenetically controlled temporal PI3K signaling activation patterns. STAR Protoc. 4 (4): 102622 (2023). PMID 38059654. [PubMed] [Article] [RRC of NBRP] |
| user_report | Meshitsuka, S., CRISPR/Cas9 and AAV mediated insertion of β2 microglobulin-HLA-G fusion gene protects mesenchymal stromal cells from allogeneic rejection and potentiates the use for off-the-shelf cell therapy. Regen. Ther. 21: 442-452 (2022). PMID 36313397. [PubMed] [Article] [RRC of NBRP] |
| user_report | Ogawa, Y., Endogenously expressed Ranbp2 is not at the axon initial segment. J. Cell Sci. 134 (6): jcs256180 (2021). PMID 33536249. [PubMed] [Article] [RRC of NBRP] |
| user_report | Suzuk,i N., TP53/p53-FBXO22-TFEB controls basal autophagy to govern hormesis. Autophagy 17 (11): 3776-3793 (2021). PMID 33706682. [PubMed] [Article] [RRC of NBRP] |
| user_report | Nanba, D., EGFR-mediated epidermal stem cell motility drives skin regeneration through COL17A1 proteolysis. J. Cell Biol. 220 (11): e202012073 (2021). PMID 34550317. [PubMed] [Article] [RRC of NBRP] |
| user_report | Girao, H., CLASP2 binding to curved microtubule tips promotes flux and stabilizes kinetochore attachments. J. Cell Biol. 219 (2). pii: e201905080 (2020). PMID 31757788. [PubMed] [Article] [RRC of NBRP] |
| user_report | Lu, F., Downregulation of TMOD1 promotes cell motility and cell proliferation in cervical cancer cells. Oncol. Lett. 19: 3339-3348 (2020). PMID 32218869. [PubMed] [Article] [RRC of NBRP] |
| user_report | Kinoshita, N., Genetically Encoded Fluorescent Indicator GRAPHIC Delineates Intercellular Connections. iScience 15: 28-38 (2019). PMID 31026667. [PubMed] [Article] [RRC of NBRP] |
| user_report | Ishikawa, T., Physiological role of urothelial cancer-associated one long noncoding RNA in human skeletogenic cell differentiation. J. Cell. Physiol. 233 (6): 4825-4840 (2018). PMID 29150954. [PubMed] [Article] [RRC of NBRP] |
| user_report | Hamada, M., Integration Mapping of piggyBac-Mediated CD19 Chimeric Antigen Receptor T Cells Analyzed by Novel Tagmentation-Assisted PCR. EBioMedicine 34: 18-26 (2018). PMID 30082227. [PubMed] [Article] [RRC of NBRP] |
| user_report | Tanaka, M., Aggregation of scaffolding protein DISC1 dysregulates phosphodiesterase 4 in Huntington's disease. J. Clin. Invest. 127 (4): 1438-1450 (2017). PMID 28263187. [PubMed] [Article] [RRC of NBRP] |
| user_report | Ujino, S., Hepatitis C virus utilizes VLDLR as a novel entry pathway. Proc. Natl. Acad. Sci. U.S.A. 113 (1): 188-193 (2016). PMID 26699506. [PubMed] [Article] [RRC of NBRP] |
| user_report | Maishi, N., Tumour endothelial cells in high metastatic tumours promote metastasis via epigenetic dysregulation of biglycan. Sci. Rep. 6: 28039 (2016). PMID 27295191. [PubMed] [Article] [RRC of NBRP] |
| user_report | Maki, T., CLASP2 Has Two Distinct TOG Domains That Contribute Differently to Microtubule Dynamics. J. Mol. Biol. 427 (14): 2379-2395 (2015). PMID 26003921. [PubMed] [Article] [RRC of NBRP] |
| user_report | Watanabe, K., FBXO21 mediates the ubiquitylation and proteasomal degradation of EID1. Genes Cells 20 (8): 667-674 (2015). PMID 26085330. [PubMed] [Article] [RRC of NBRP] |
| user_report | Masuda, T., Fate of HIV-1 cDNA intermediates during reverse transcription is dictated by transcription initiation site of virus genomic RNA. Sci. Rep. 5: 17680 (2015). PMID 26631448. [PubMed] [Article] [RRC of NBRP] |
| user_report | Uemura, S., Expression machinery of GM4: the excess amounts of GM3/GM4S synthase (ST3GAL5) are necessary for GM4 synthesis in mammalian cells. Glycoconj. J. 31 (2): 101-108 (2014). PMID 23999868. [PubMed] [Article] [RRC of NBRP] |
| user_report | Ohnishi, T., Identification and characterization of a neuron-specific isoform of protrudin. Genes Cells 19 (2): 97-111 (2014). PMID 24251978. [PubMed] [Article] [RRC of NBRP] |
| user_report | Araya, N., HTLV-1 induces a Th1-like state in CD4+CCR4+ T cells. J. Clin. Invest., 124 (8): 3431-42 (2014). PMID 24960164. [PubMed] [Article] [RRC of NBRP] |
| user_report | Grimaldi, A.D., CLASPs are required for proper microtubule localization of end-binding proteins. Dev. Cell 30 (3): 343-352 (2014). PMID 25117684. [PubMed] [Article] [RRC of NBRP] |
| user_report | Ishibashi, M., Clinical significance of the expression of long non-coding RNA HOTAIR in primary hepatocellular carcinoma. Oncol. Rep. 29 (3): 946-950 (2013). PMID 23292722. [PubMed] [Article] [RRC of NBRP] |
| user_report | Takahashi, Y., Paired related homoeobox 1, a new EMT inducer, is involved in metastasis and poor prognosis in colorectal cancer. Br. J. Cancer, 109 (2): 307-311 (2013). PMID 23807160. [PubMed] [Article] [RRC of NBRP] |
| user_report | Watanabe, T., FBL2 regulates amyloid precursor protein (APP) metabolism by promoting ubiquitination-dependent APP degradation and inhibition of APP endocytosis. J. Neurosci., 32 (10): 3352-3365 (2012). PMID 22399757. [PubMed] [Article] [RRC of NBRP] |
| user_report | Kanemura H., Impaired vascular development in the yolk sac and allantois in mice lacking RA-GEF-1. Biochem. Biophys. Res. Commun., 387, 754-759 (2009). PMID 19635461. [PubMed] [Article] [RRC of NBRP] |
| user_report | Sakurai, Y., DNA double strand break repair enzymes function at multiple steps in retroviral infection. Retrovirology, 6:114 (2009). PMID 20003485. [PubMed] [Article] [RRC of NBRP] |
Resource search
Link
Other resources in our bankExternal Database
Reference sequence
Tips
Featured content
- Lentiviral plasmid list (English text)
- Lentivirus Vector (English text)
- Lentivirus Vector (Japanese text)
