Resource data sheet
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pCMV-SP1

Expression vector of human SP1

Catalog number RDB04267
Resource name pCMV-SP1
Clone info. Expression clone of human SP1 cDNA.
CDS segment 98..363 of NM_138473.2 is not included. This clone contains mouse Trp53 3'UTR (NM_011640.3 1331..1613) and a portion of pGL2 basic (X65323.2 1891..2743) between the SP1 CDS and BGH poly(A) site.
Vector backbone pcDNA3 (Plasmid)
Selectable markers Amp^r
Gene/insert name Human Sp1 cDNA
Depositor Yokoyama, Kazunari | Song, Jun |
 
Other clones in our bank human SP1 (NCBI Gene 6667) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR Manuscripts for the publication shall be made through the discussion between Depositor and Recipient Investigator. Recipient investigator shall include Depositor/Developer as co-author in manuscript for the publication.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Song, J. et al., J. Biol. Chem. 276 (23): 19897-19904, 2001).
Recipient Investigator shall negotiate with Developer/Depositor when patent application and the commercialization for the products based upon Material, derivatives thereof their use is considered.
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RDB04267 pCMV-SP1 DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Featured content

References

original Song, J., Independent repression of a GC-rich housekeeping gene by Sp1 and MAZ involves the same cis-elements. J. Biol. Chem., 276, 19897-19904 (2001). PMID 11259406.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB12832_A4Gp.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Foward RDB12832_A4Gpa.seq
>04267_12832_A4Gp_CMV-Foward_D01_10.ab1
    1 CCCGATCGTC ACCTCCGCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC
   61 TATATAAGCA GAGCTCGTTT AGTGAACCGT CAGATCGCCT GGAGACGCCA TCCACGCTGT
  121 TTTGACCTCC ATAGAAGACA CCGGGACCGA TCCAGCCTCC CCGGCTAGCC ATGGCTTCAA
  181 TGACAGGGGG ACAACAAATG GGGCGAGGGC ATCATCATCA TCATCATGCG CGCCGAGCAT
  241 CAGTAGGGGT TACAGGTGAG CTTGACCTCA CAGCCACACA ACTTTCACAG GGTGCCAATG
  301 GCTGGCAGAT CATCTCTTCC TCCTCTGGGG CTACCCCTAC CTCAAAGGAA CAGAGTGGCA
  361 GCAGTACCAA TGGCAGCAAT GGCAGTGAGT CTTCCAAGAA TCGCACAGTC TCTGGTGGGC
  421 AGTATGTTGT GGCTGCCGCT CCCAACTTAC AGAACCAGCA AGTTCTGACA GGACTACCTG
  481 GAGTGATGCC TAATATTCAG TATCAAGTAA TCCCACAGTT CCAGACCGTT GATGGGCAAC
  541 AGCTGCAGTT TGCTGCCACT GGGGCCCAAG TGCAGCAGGA TGGTTCTGGT CAAATACAGA
  601 TCATACCAGG TGCAAACCAA CAGATTATCA CAAATCGAGG AAGTGGAGGC AACATCATTG
  661 CTGCTATGCC AAACCTACTC CAGCAGGCTG TCCCCCTCCA AGGCCTGGCT AATAATGTAC
  721 TCTCAGGACA GACTCAGTAT GTGACCAATG TACCAGTGGC CCTGAATGGG AACATCACCT
  781 TGCTACCTGT CAACAGCGTT TCTGCAGCTA CCTTGACTCC CAGCTCTCAG GCAGTCACGA
  841 TCAGCAGCTC TGGGTCCCAG GAGAGTGGCT CACAGCCTGT CACCTCAGGG ACTACCATCA
  901 GTTCTGCCAG CTTGGTATCA TCACAAGCCA GTTCCAGCTC CTTTTTCACC AATGCCAATA
  961 GCTACTCAAC TACTACTACC ACCAGCAACA TGGGAATTAT GAACTTTACT ACCAGTGGGA
 1021 TCATCAGGAC CAACTCTCAA GGCCAGACAC CCCAGAGGGT CAGTGGGGCT ACAGGGGTCT
 1081 GATGCTCTGA CATCCAGCAA AACCAGACAT CTGAAGCTCA TGCAGCAAGG CCCAGCCAAA
1141 AGAGAGAAGA GAGCCAAGAT A
//
Primer: BGH_rev RDB12832_A4Gpb.seq
>04267_12832_A4Gp_BGH_rev_E01_13.ab1
    1 CGTAAAAGTT AATCACGCGG TGGCGGCCGC TCTAGAACTA GTGGATCGAT CCAGACATGA
   61 TAAGATACAT TGATGAGTTT GGACAAACCA CAACTAGAAT GCAGTGAAAA AAATGCTTTA
  121 TTTGTGAAAT TTGTGATGCT ATTGCTTTAT TTGTAACCAT TATAAGCTGC AATAAACAAA
  181 TTAACAACAA CAATTGCATT CATTTTATGT TTCAGGTTCA GGGGGAGGTG TGGGAGGTTT
  241 TTTAAAGCAA ATAAAACCTC TACAAATGTG GTATGGCTGA TTATGATCTC TAGTCAAGGC
  301 ACTATACATC AAATATTCCT TATTAACCCC TTTACAAATT AAAAAGCTAA AGGTACACAA
  361 TTTTTGAGCA TAGTTATTAA TAGCAGACAC TCTATGCCTG TGTGGAGTAA GAAAAAACAG
  421 TATGTTATGA TTATAACTGT TATGCCTACT TATAAAGGTT ACAGAATATT TTTCCATAAT
  481 TTTCTTGTAT AGCAGTGCAG CTTTTTCCTT TGTGGTGTAA ATAGCAAAGC AAGCAAGAGT
  541 TCTATTACTA AACACAGCAT GACTCAAAAA ACTTAGCAAT TCTGAAGGAA AGTCCTTGGG
  601 GTCTTCTACC TTTCTCTTCT TTTTTGGAGG AGTAGAATGT TGAGAGTCAG CAGTAGCCTC
  661 ATCATCACTA GATGGCATTT CTTCTGAGCA AAACAGGTTT TCCTCATTAA AGGCATTCCA
  721 CCACTGCTCC CATTCATCAG TTCCATAGGT TGGAAATCTA AAATACACAA ACAATTAGAA
  781 TCAGTAGTTT AACACATTAT ACACTTAAAA ATTTTATATT TACCTTAGAG CTTTAAATCT
  841 CTGTAGGTAG TTTGTCCAAT TATGTCACAC CACAGAAGTA AGGTTCCTTT CACAAAGATC
  901 CGAGCTTGGC CTGCAGGTCC AAGATAGAAT CTTACAGGGT GTGGGGTAGG GTGAAGATTT
  961 CATTGTAGGT GCCAGGGTCC AACAACCTGG GCTGGATAGA ATTTCGCTGG GTCAGCAGAA
 1021 GACCCTGACC AACTAATCAA CCCTATTCCC CAACTTGGGG CCAGGAAACC ACTTACTTCA
 1081 GAAAGGGGCC TGAGCCTTAG CCTACAAGTA AAAAAAGGCA GCAGAAAGGA ACTGGAAGAT
1141 GTGTTCTCAG GCCTTGAGTC ATAAGAACAG CCCAAGAAAA AGTGGGGAAA GGCT
//
Primer: pMAMneo_Reverse RDB12832_A4Gpc.seq
>04267_12832_A4Gp_pMAMneo_Reverse_B04_04.ab1
    1 TTATCAAGTA TGTCACACCA CAGAAGTAAG GTTCCTTCAC AAAGATCCGA GCTTGGCTGC
   61 AGGTCCAAGA TAGAATCTTA CAGGGTGTGG GGTAGGGTGA GATTTCATTG TAGGTGCCAG
  121 GGTCCAACAA CTGGCTGGAT AGAATTTCGC TGGGCCAGCA GAGACCTGAC AACTATCAAC
  181 CTATTCCCCA ACTTGGGCCA GGAACCACTA CTCAGAGAGG GGGCTGAGCC CTAGCTACAA
  241 GGTAAAAAAG GCAGCAGAAG GGACCGGGAG GATTGTGTCT CAGCCCTGAA GTCATAAGAC
  301 AGCAAGGAGA GGGGGAGGCT GGTGATGGGG ACGGGATGCA GAGGCAGGGA TCCTTAAGCT
  361 TTCAGAAGCC ATTGCCACTG ATATTAATGG ACTGCAGATC TGCCACCTGC ATGACGTTGA
  421 TGCCACTGTT GGCAAGACGG GCAATGCCCT CTGGACAGAT GGCCTCCATG GCTACCATAT
  481 TGGTGGTAAT AAGGGCTGAA GGAGTGGCAG TGCCACTGCC TTCTGAACCT GCCCCACTGT
  541 CCAGGGGCAA AGTGCCCACA CTCAGAGCTA CACCTGGGCC TCCCTTCTTA TTCTGGTGGG
  601 TCTTGATATG TTTTGACAGG TGGTCACTCC TCATGAAGCG CTTAGGACAC TCAGGGCAGG
  661 CAAATTTCTT CTCACCTGTG TGTGTACGTT TGTGCCTCTG TAGCTCATCC GAACGTGTGA
  721 AGCGTTTCCC ACAGTATGAC CAGGTACACA TAAATGGCCT CTCGCCTGTA TGCCAGCGCA
  781 AGTGTGCCCG CAGGTGAGAG GTCTTGCCAT ACACTTTCCC ACAGCCTTGG ATGTGGCAAA
  841 TATGCTGTTT CTTTTTGCCA GGATCCCCCG AGCCCCTTCC TTCACTGTCT TTACAGTAGG
  901 GGCAGGTGCA TGCTTCCCGC CGGGTCCTCC GACCGGCTTG GGGTTGGGCA TCTGGGCTGT
  961 TTTCTCCTTC CTCTCCACCC TGCTGTGTCA TCATGTATTC CATCACCCAC CAGCCCCATG
 1021 GTAGACCCAA GCTGAGCTCC CATGATCACC TGGGGCATTT GCTATAGCCC ACCGCCAAGA
 1081 CTTGATTGGG TGCACCTGCA TCTGAAGTAC CGATGCACTG AGCTTAATGG ATTCTGGGAA
1141 GGACCTG
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Song, J., Independent repression of a GC-rich housekeeping gene by Sp1 and MAZ involves the same cis-elements. J. Biol. Chem., 276, 19897-19904 (2001). PMID 11259406.
user report Membrino, A., G4-DNA Formation in the HRAS Promoter and Rational Design of Decoy Oligonucleotides for Cancer Therapy. PLoS One. 6 (9): e24421 (2011). PMID 21931711.
user report Frensing T., Characterization of a neuregulin-1 gene promoter: positive regulation of type I isoforms by NF-kappaB. Biochim. Biophys. Acta, 1779, 139-144 (2008). PMID 18082154.

2018.12.11

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