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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pCMV-SP1

Expression vector of human SP1

Catalog number RDB04267
Resource name pCMV-SP1
Clone info. Expression clone of human SP1 cDNA.
Comment CDS segment 56..301nt of NM_003109.1 (CDS: 56..2392nt) is not included. This clone contains mouse Trp53 3'UTR (NM_011640.3 1331..1613) and SV40 poly(A) signal after SP1 CDS.
Vector backbone pcDNA3 (Plasmid)
Selectable markers Amp^r
Gene/insert name Human Sp1 cDNA
Depositor|Developer Yokoyama, Kazunari | Song, Jun |
Other clones in our bank
External Database
Human Sp1
          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR Manuscripts for the publication shall be made through the discussion between Depositor and Recipient Investigator. Recipient investigator shall include Depositor/Developer as co-author in manuscript for the publication.
          In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Song, J. et al., J. Biol. Chem. 276 (23): 19897-19904, 2001).
          Recipient Investigator shall negotiate with Developer/Depositor when patent application and the commercialization for the products based upon Material, derivatives thereof their use is considered.
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          提供条件 使用にあたっては常に密な連絡をとり、発表等に関しても 事前に合意を要する。共著とする。
          利用者は、研究成果の公表にあたって寄託者の指 定する文献を引用する(Song, J. et al., J. Biol. Chem. 276 (23): 19897-19904, 2001) 。
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          RDB04267 pCMV-SP1 DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Song, J., Independent repression of a GC-rich housekeeping gene by Sp1 and MAZ involves the same cis-elements. J. Biol. Chem., 276, 19897-19904 (2001). PMID 11259406.

          Further references such as user reports and related articles (go to bottom)

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          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB12832_A4Gpp2-2.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: CMV-Foward
          Sequence file: RDB12832_A4Gpa.seq check
          >04267_12832_A4Gp_CMV-Foward_D01_10.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNTNNNGCNN ATGGGCGGTA GGCGTGNNCG GTGGGAGGTC
             61 TATATAAGCA GAGCTCGNTN NNNGAACCGT CAGATCGCCT GGAGACGCCA TCCACGCTGT
            121 TTTGACCTCC ATAGAAGACA CCGGGACCGA TCCAGCCTCC CCGGCTAGCC ATGGCTTCAA
            181 TGACAGGGGG ACAACAAATG GGGCGAGGGC ATCATCATCA TCATCATGCG CGCCGAGCAT
            241 CAGTAGGGGT TACAGGTGAG CTTGACCTCA CAGCCACACA ACTTTCACAG GGTGCCAATG
            301 GCTGGCAGAT CATCTCTTCC TCCTCTGGGG CTACCCCTAC CTCAAAGGAA CAGAGTGGCA
            361 GCAGTACCAA TGGCAGCAAT GGCAGTGAGT CTTCCAAGAA TCGCACAGTC TCTGGTGGGC
            421 AGTATGTTGT GGCTGCCGCT CCCAACTTAC AGAACCAGCA AGTTCTGACA GGACTACCTG
            481 GAGTGATGCC TAATATTCAG TATCAAGTAA TCCCACAGTT CCAGACCGTT GATGGGCAAC
            541 AGCTGCAGTT TGCTGCCACT GGGGCCCAAG TGCAGCAGGA TGGTTCTGGT CAAATACAGA
            601 TCATACCAGG TGCAAACCAA CAGATTATCA CAAATCGAGG AAGTGGAGGC AACATCATTG
            661 CTGCTATGCC AAACCTACTC CAGCAGGCTG TCCCCCTCCA AGGCCTGGCT AATAATGTAC
            721 TCTCAGGACA GACTCAGTAT GTGACCAATG TACCAGTGGC CCTGAATGGG AACATCACCT
            781 TGCTACCTGT CAACAGCGTT TCTGCAGCTA CCTTGACTCC CAGCTCTCAN GCAGTCACGA
            841 TCAGCAGCTC TGGNNCCCAN GANAGTGNNT CACAGCCNGN CNCCTCNNGG ANTANNNTCA
            901 GTTCTGCCAG CTTNNNATCA TCACAAGCCN NTTCCAGCTC CTTTTTNNNN AATGNNNATA
            961 GCTACTCNNN NACNACNACC NNNNGNNNNN NNGNNNNNNN GNNNNTNACN ANNNNNNGNN
          1021 NNNNNNNN
          //
          Primer: pMAMneo_Reverse
          Sequence file: RDB12832_A4Gpb.seq check
          >04267_12832_A4Gp_pMAMneo_Reverse_B04_04.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNGAAGTNNG GNTCCTTCNC NAAGANCCGA GCTTGGCTGC
             61 AGGTCCAAGA TAGAATCTTA CAGGGTGTGG GGTAGGGTGA GATTTCATTG TAGGTGCCAG
            121 GGTCCAACAA CTGGCTGGAT AGAATTTCGC TGGGCCAGCA GAGACCTGAC AACTATCAAC
            181 CTATTCCCCA ACTTGGGCCA GGAACCACTA CTCAGAGAGG GGGCTGAGCC CTAGCTACAA
            241 GGTAAAAAAG GCAGCAGAAG GGACCGGGAG GATTGTGTCT CAGCCCTGAA GTCATAAGAC
            301 AGCAAGGAGA GGGGGAGGCT GGTGATGGGG ACGGGATGCA GAGGCAGGGA TCCTTAAGCT
            361 TTCAGAAGCC ATTGCCACTG ATATTAATGG ACTGCAGATC TGCCACCTGC ATGACGTTGA
            421 TGCCACTGTT GGCAAGACGG GCAATGCCCT CTGGACAGAT GGCCTCCATG GCTACCATAT
            481 TGGTGGTAAT AAGGGCTGAA GGAGTGGCAG TGCCACTGCC TTCTGAACCT GCCCCACTGT
            541 CCAGGGGCAA AGTGCCCACA CTCAGAGCTA CACCTGGGCC TCCCTTCTTA TTCTGGTGGG
            601 TCTTGATATG TTTTGACAGG TGGTCACTCC TCATGAAGCG CTTAGGACAC TCAGGGCAGG
            661 CAAATTTCTT CTCACCTGTG TGTGTACGTT TGTGCCTCTG TAGCTCATCC GAACGTGTGA
            721 AGCGTTTCCC ACAGTATGAC CAGGTACACA TAAATGGCCT CTCGCCTGTA TGCCAGCGCA
            781 AGTGTGCCCG CANGTGAGAG GTCTTGCCAT ACACTTTCCC ACAGCCTTNG ATGTGGCAAA
            841 TATGCTGTTT CTTTTTGNCN GGATCCNNCN AGCCNNNTNC TTCANTNNCT TTACNGNANG
            901 GGCANGTGCA TGCTTCCCGC CNGGNCCNNN NANCGGCTTG GGGNTGGNNA TCTNGNCTGT
          961 TTNCNNCTNN CNNNNNNNCN TGNNNNNNNA TCNNGNNNNN CNNNNNCNNN NNNNNNN
          //

          Please visit Sequencing and PCR primers for primer information.

          References

          Original, user report and related articles

          original Song, J., Independent repression of a GC-rich housekeeping gene by Sp1 and MAZ involves the same cis-elements. J. Biol. Chem., 276, 19897-19904 (2001). PMID 11259406.
          user_report Membrino, A., G4-DNA Formation in the HRAS Promoter and Rational Design of Decoy Oligonucleotides for Cancer Therapy. PLoS One. 6 (9): e24421 (2011). PMID 21931711.
          user_report Frensing T., Characterization of a neuregulin-1 gene promoter: positive regulation of type I isoforms by NF-kappaB. Biochim. Biophys. Acta, 1779, 139-144 (2008). PMID 18082154.

          2022.05.18

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