RDB04267 - pCMV-SP1

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

pCMV-SP1 (#RDB04267)

Expression vector of human SP1

Clone info.	Expression clone of human SP1 cDNA.
Comment	CDS segment 56..301nt of NM_003109.1 (CDS: 56..2392nt) is not included. This clone contains mouse Trp53 3'UTR (NM_011640.3 1331..1613) and SV40 poly(A) signal after SP1 CDS.
Vector backbone	pcDNA3 (Plasmid)
Selectable markers	Amp^r
Gene/insert name	Human Sp1 cDNA
Depositor\|Developer	Yokoyama, Kazunari \| Song, Jun \|

Distribution information

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Terms and conditions for distribution Manuscripts for the publication shall be made through the discussion between Depositor and Recipient Investigator. Recipient investigator shall include Depositor/Developer as co-author in manuscript for the publication.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Song, J. et al., J. Biol. Chem. 276 (23): 19897-19904, 2001).
Recipient Investigator shall negotiate with Developer/Depositor when patent application and the commercialization for the products based upon Material, derivatives thereof their use is considered.

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Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.
Terms and conditions for distribution	Manuscripts for the publication shall be made through the discussion between Depositor and Recipient Investigator. Recipient investigator shall include Depositor/Developer as co-author in manuscript for the publication. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Song, J. et al., J. Biol. Chem. 276 (23): 19897-19904, 2001). Recipient Investigator shall negotiate with Developer/Depositor when patent application and the commercialization for the products based upon Material, derivatives thereof their use is considered.

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件	使用にあたっては常に密な連絡をとり、発表等に関しても事前に合意を要する。共著とする。利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Song, J. et al., J. Biol. Chem. 276 (23): 19897-19904, 2001) 。本リソースを使用して商業利用する場合は、寄託者と改めて契約する。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB04267	pCMV-SP1	DNA solution

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pCMV-SP1 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB04267).

Reference section:

Song, J., Ugai, H., Kanazawa, I., Sun, K., Yokoyama, K.K., Independent repression of a GC-rich housekeeping gene by Sp1 and MAZ involves the same cis-elements. J. Biol. Chem., 276, 19897-19904 (2001). PMID 11259406. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB12832_A4Gpp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Foward () Sequence file: RDB12832_A4Gpa.seq
>04267_12832_A4Gp_CMV-Foward_D01_10.ab1 1 NNNNNNNNNN NNNNNNNNNN NNTNNNGCNN ATGGGCGGTA GGCGTGNNCG GTGGGAGGTC 61 TATATAAGCA GAGCTCGNTN NNNGAACCGT CAGATCGCCT GGAGACGCCA TCCACGCTGT 121 TTTGACCTCC ATAGAAGACA CCGGGACCGA TCCAGCCTCC CCGGCTAGCC ATGGCTTCAA 181 TGACAGGGGG ACAACAAATG GGGCGAGGGC ATCATCATCA TCATCATGCG CGCCGAGCAT 241 CAGTAGGGGT TACAGGTGAG CTTGACCTCA CAGCCACACA ACTTTCACAG GGTGCCAATG 301 GCTGGCAGAT CATCTCTTCC TCCTCTGGGG CTACCCCTAC CTCAAAGGAA CAGAGTGGCA 361 GCAGTACCAA TGGCAGCAAT GGCAGTGAGT CTTCCAAGAA TCGCACAGTC TCTGGTGGGC 421 AGTATGTTGT GGCTGCCGCT CCCAACTTAC AGAACCAGCA AGTTCTGACA GGACTACCTG 481 GAGTGATGCC TAATATTCAG TATCAAGTAA TCCCACAGTT CCAGACCGTT GATGGGCAAC 541 AGCTGCAGTT TGCTGCCACT GGGGCCCAAG TGCAGCAGGA TGGTTCTGGT CAAATACAGA 601 TCATACCAGG TGCAAACCAA CAGATTATCA CAAATCGAGG AAGTGGAGGC AACATCATTG 661 CTGCTATGCC AAACCTACTC CAGCAGGCTG TCCCCCTCCA AGGCCTGGCT AATAATGTAC 721 TCTCAGGACA GACTCAGTAT GTGACCAATG TACCAGTGGC CCTGAATGGG AACATCACCT 781 TGCTACCTGT CAACAGCGTT TCTGCAGCTA CCTTGACTCC CAGCTCTCAN GCAGTCACGA 841 TCAGCAGCTC TGGNNCCCAN GANAGTGNNT CACAGCCNGN CNCCTCNNGG ANTANNNTCA 901 GTTCTGCCAG CTTNNNATCA TCACAAGCCN NTTCCAGCTC CTTTTTNNNN AATGNNNATA 961 GCTACTCNNN NACNACNACC NNNNGNNNNN NNGNNNNNNN GNNNNTNACN ANNNNNNGNN 1021 NNNNNNNN //
Primer: pMAMneo_Reverse () Sequence file: RDB12832_A4Gpb.seq
>04267_12832_A4Gp_pMAMneo_Reverse_B04_04.ab1 1 NNNNNNNNNN NNNNNNNNNN NNGAAGTNNG GNTCCTTCNC NAAGANCCGA GCTTGGCTGC 61 AGGTCCAAGA TAGAATCTTA CAGGGTGTGG GGTAGGGTGA GATTTCATTG TAGGTGCCAG 121 GGTCCAACAA CTGGCTGGAT AGAATTTCGC TGGGCCAGCA GAGACCTGAC AACTATCAAC 181 CTATTCCCCA ACTTGGGCCA GGAACCACTA CTCAGAGAGG GGGCTGAGCC CTAGCTACAA 241 GGTAAAAAAG GCAGCAGAAG GGACCGGGAG GATTGTGTCT CAGCCCTGAA GTCATAAGAC 301 AGCAAGGAGA GGGGGAGGCT GGTGATGGGG ACGGGATGCA GAGGCAGGGA TCCTTAAGCT 361 TTCAGAAGCC ATTGCCACTG ATATTAATGG ACTGCAGATC TGCCACCTGC ATGACGTTGA 421 TGCCACTGTT GGCAAGACGG GCAATGCCCT CTGGACAGAT GGCCTCCATG GCTACCATAT 481 TGGTGGTAAT AAGGGCTGAA GGAGTGGCAG TGCCACTGCC TTCTGAACCT GCCCCACTGT 541 CCAGGGGCAA AGTGCCCACA CTCAGAGCTA CACCTGGGCC TCCCTTCTTA TTCTGGTGGG 601 TCTTGATATG TTTTGACAGG TGGTCACTCC TCATGAAGCG CTTAGGACAC TCAGGGCAGG 661 CAAATTTCTT CTCACCTGTG TGTGTACGTT TGTGCCTCTG TAGCTCATCC GAACGTGTGA 721 AGCGTTTCCC ACAGTATGAC CAGGTACACA TAAATGGCCT CTCGCCTGTA TGCCAGCGCA 781 AGTGTGCCCG CANGTGAGAG GTCTTGCCAT ACACTTTCCC ACAGCCTTNG ATGTGGCAAA 841 TATGCTGTTT CTTTTTGNCN GGATCCNNCN AGCCNNNTNC TTCANTNNCT TTACNGNANG 901 GGCANGTGCA TGCTTCCCGC CNGGNCCNNN NANCGGCTTG GGGNTGGNNA TCTNGNCTGT 961 TTNCNNCTNN CNNNNNNNCN TGNNNNNNNA TCNNGNNNNN CNNNNNCNNN NNNNNNN //
Primer: f1ori_F () Sequence file: RDB12832_A4Gpc.seq
>04267_12832_B1DlS_TP53-UTR_F_E01_05_ABI08.ab1 1 NNNNNNNNNN NNNNNNCCCN NNNCCCNNNN NGANTCTATC TTGGACCTGC AGCCAAGCTC 61 GGATCTTTGT GAAGGAACCT TACTTCTGTG GTGTGACATA ATTGGACAAA CTACCTACAG 121 AGATTTAAAG CTCTAAGGTA AATATAAAAT TTTTAAGTGT ATAATGTGTT AAACTACTGA 181 TTCTAATTGT TTGTGTATTT TAGATTCCAA CCTATGGAAC TGATGAATGG GAGCAGTGGT 241 GGAATGCCTT TAATGAGGAA AACCTGTTTT GCTCANAANA AATGCCATCT AGTGATGATG 301 AGGCTACTGC TGACTCTCAN NNNTNNNNNN NTCCAAANNN NANNANAANG GNNNAANANC 361 CCNAGGACNT TCCTTCNNAA TTNNNNNNTT NNNNGAGN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original	Song, J., Independent repression of a GC-rich housekeeping gene by Sp1 and MAZ involves the same cis-elements. J. Biol. Chem., 276, 19897-19904 (2001). PMID 11259406. [link to RRC of NBRP]
user_report	Membrino, A., G4-DNA Formation in the HRAS Promoter and Rational Design of Decoy Oligonucleotides for Cancer Therapy. PLoS One. 6 (9): e24421 (2011). PMID 21931711. [link to RRC of NBRP]
user_report	Frensing T., Characterization of a neuregulin-1 gene promoter: positive regulation of type I isoforms by NF-kappaB. Biochim. Biophys. Acta - Gene Regul. Mech., 1779, 139-144 (2008). PMID 18082154. [link to RRC of NBRP]

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human Sp1

External Database
Human Sp1

Reference sequence