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pCMV-SP1 (#RDB04267)

Expression vector of human SP1

Clone info. Expression clone of human SP1 cDNA.
Comment CDS segment 56..301nt of NM_003109.1 (CDS: 56..2392nt) is not included. This clone contains mouse Trp53 3'UTR (NM_011640.3 1331..1613) and SV40 poly(A) signal after SP1 CDS.
Vector backbone pcDNA3 (Plasmid)
Selectable markers Amp^r
Gene/insert name Human Sp1 cDNA
Depositor|Developer Yokoyama, Kazunari | Song, Jun |
 

Distribution information

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Terms and conditions for distribution Manuscripts for the publication shall be made through the discussion between Depositor and Recipient Investigator. Recipient investigator shall include Depositor/Developer as co-author in manuscript for the publication.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Song, J. et al., J. Biol. Chem. 276 (23): 19897-19904, 2001).
Recipient Investigator shall negotiate with Developer/Depositor when patent application and the commercialization for the products based upon Material, derivatives thereof their use is considered.
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手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 使用にあたっては常に密な連絡をとり、発表等に関しても 事前に合意を要する。共著とする。
利用者は、研究成果の公表にあたって寄託者の指 定する文献を引用する(Song, J. et al., J. Biol. Chem. 276 (23): 19897-19904, 2001) 。
本リソースを 使用して商業利用する場合は、寄託者と改めて契約する。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB04267 pCMV-SP1 DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pCMV-SP1 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB04267).

Reference section:

Song, J., Ugai, H., Kanazawa, I., Sun, K., Yokoyama, K.K., Independent repression of a GC-rich housekeeping gene by Sp1 and MAZ involves the same cis-elements. J. Biol. Chem., 276, 19897-19904 (2001). PMID 11259406. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB12832_A4Gpp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Foward ()
Sequence file: RDB12832_A4Gpa.seq check
>04267_12832_A4Gp_CMV-Foward_D01_10.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNTNNNGCNN ATGGGCGGTA GGCGTGNNCG GTGGGAGGTC
   61 TATATAAGCA GAGCTCGNTN NNNGAACCGT CAGATCGCCT GGAGACGCCA TCCACGCTGT
  121 TTTGACCTCC ATAGAAGACA CCGGGACCGA TCCAGCCTCC CCGGCTAGCC ATGGCTTCAA
  181 TGACAGGGGG ACAACAAATG GGGCGAGGGC ATCATCATCA TCATCATGCG CGCCGAGCAT
  241 CAGTAGGGGT TACAGGTGAG CTTGACCTCA CAGCCACACA ACTTTCACAG GGTGCCAATG
  301 GCTGGCAGAT CATCTCTTCC TCCTCTGGGG CTACCCCTAC CTCAAAGGAA CAGAGTGGCA
  361 GCAGTACCAA TGGCAGCAAT GGCAGTGAGT CTTCCAAGAA TCGCACAGTC TCTGGTGGGC
  421 AGTATGTTGT GGCTGCCGCT CCCAACTTAC AGAACCAGCA AGTTCTGACA GGACTACCTG
  481 GAGTGATGCC TAATATTCAG TATCAAGTAA TCCCACAGTT CCAGACCGTT GATGGGCAAC
  541 AGCTGCAGTT TGCTGCCACT GGGGCCCAAG TGCAGCAGGA TGGTTCTGGT CAAATACAGA
  601 TCATACCAGG TGCAAACCAA CAGATTATCA CAAATCGAGG AAGTGGAGGC AACATCATTG
  661 CTGCTATGCC AAACCTACTC CAGCAGGCTG TCCCCCTCCA AGGCCTGGCT AATAATGTAC
  721 TCTCAGGACA GACTCAGTAT GTGACCAATG TACCAGTGGC CCTGAATGGG AACATCACCT
  781 TGCTACCTGT CAACAGCGTT TCTGCAGCTA CCTTGACTCC CAGCTCTCAN GCAGTCACGA
  841 TCAGCAGCTC TGGNNCCCAN GANAGTGNNT CACAGCCNGN CNCCTCNNGG ANTANNNTCA
  901 GTTCTGCCAG CTTNNNATCA TCACAAGCCN NTTCCAGCTC CTTTTTNNNN AATGNNNATA
  961 GCTACTCNNN NACNACNACC NNNNGNNNNN NNGNNNNNNN GNNNNTNACN ANNNNNNGNN
1021 NNNNNNNN
//
Primer: pMAMneo_Reverse ()
Sequence file: RDB12832_A4Gpb.seq check
>04267_12832_A4Gp_pMAMneo_Reverse_B04_04.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNGAAGTNNG GNTCCTTCNC NAAGANCCGA GCTTGGCTGC
   61 AGGTCCAAGA TAGAATCTTA CAGGGTGTGG GGTAGGGTGA GATTTCATTG TAGGTGCCAG
  121 GGTCCAACAA CTGGCTGGAT AGAATTTCGC TGGGCCAGCA GAGACCTGAC AACTATCAAC
  181 CTATTCCCCA ACTTGGGCCA GGAACCACTA CTCAGAGAGG GGGCTGAGCC CTAGCTACAA
  241 GGTAAAAAAG GCAGCAGAAG GGACCGGGAG GATTGTGTCT CAGCCCTGAA GTCATAAGAC
  301 AGCAAGGAGA GGGGGAGGCT GGTGATGGGG ACGGGATGCA GAGGCAGGGA TCCTTAAGCT
  361 TTCAGAAGCC ATTGCCACTG ATATTAATGG ACTGCAGATC TGCCACCTGC ATGACGTTGA
  421 TGCCACTGTT GGCAAGACGG GCAATGCCCT CTGGACAGAT GGCCTCCATG GCTACCATAT
  481 TGGTGGTAAT AAGGGCTGAA GGAGTGGCAG TGCCACTGCC TTCTGAACCT GCCCCACTGT
  541 CCAGGGGCAA AGTGCCCACA CTCAGAGCTA CACCTGGGCC TCCCTTCTTA TTCTGGTGGG
  601 TCTTGATATG TTTTGACAGG TGGTCACTCC TCATGAAGCG CTTAGGACAC TCAGGGCAGG
  661 CAAATTTCTT CTCACCTGTG TGTGTACGTT TGTGCCTCTG TAGCTCATCC GAACGTGTGA
  721 AGCGTTTCCC ACAGTATGAC CAGGTACACA TAAATGGCCT CTCGCCTGTA TGCCAGCGCA
  781 AGTGTGCCCG CANGTGAGAG GTCTTGCCAT ACACTTTCCC ACAGCCTTNG ATGTGGCAAA
  841 TATGCTGTTT CTTTTTGNCN GGATCCNNCN AGCCNNNTNC TTCANTNNCT TTACNGNANG
  901 GGCANGTGCA TGCTTCCCGC CNGGNCCNNN NANCGGCTTG GGGNTGGNNA TCTNGNCTGT
961 TTNCNNCTNN CNNNNNNNCN TGNNNNNNNA TCNNGNNNNN CNNNNNCNNN NNNNNNN
//
Primer: f1ori_F ()
Sequence file: RDB12832_A4Gpc.seq check
>04267_12832_B1DlS_TP53-UTR_F_E01_05_ABI08.ab1
    1 NNNNNNNNNN NNNNNNCCCN NNNCCCNNNN NGANTCTATC TTGGACCTGC AGCCAAGCTC
   61 GGATCTTTGT GAAGGAACCT TACTTCTGTG GTGTGACATA ATTGGACAAA CTACCTACAG
  121 AGATTTAAAG CTCTAAGGTA AATATAAAAT TTTTAAGTGT ATAATGTGTT AAACTACTGA
  181 TTCTAATTGT TTGTGTATTT TAGATTCCAA CCTATGGAAC TGATGAATGG GAGCAGTGGT
  241 GGAATGCCTT TAATGAGGAA AACCTGTTTT GCTCANAANA AATGCCATCT AGTGATGATG
  301 AGGCTACTGC TGACTCTCAN NNNTNNNNNN NTCCAAANNN NANNANAANG GNNNAANANC
361 CCNAGGACNT TCCTTCNNAA TTNNNNNNTT NNNNGAGN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Song, J., Independent repression of a GC-rich housekeeping gene by Sp1 and MAZ involves the same cis-elements. J. Biol. Chem., 276, 19897-19904 (2001). PMID 11259406. [link to RRC of NBRP]
user_report Membrino, A., G4-DNA Formation in the HRAS Promoter and Rational Design of Decoy Oligonucleotides for Cancer Therapy. PLoS One. 6 (9): e24421 (2011). PMID 21931711. [link to RRC of NBRP]
user_report Frensing T., Characterization of a neuregulin-1 gene promoter: positive regulation of type I isoforms by NF-kappaB. Biochim. Biophys. Acta - Gene Regul. Mech., 1779, 139-144 (2008). PMID 18082154. [link to RRC of NBRP]