Resource data sheet
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pKM2L

Reporter construct with Renilla luciferase gene

Catalog number RDB04026
Resource name pKM2L
Alternative name 1142C6F
Clone info. Plasmid clone of Renilla luciferase reporter vector. The PacI fragment of pEXWS2L (W1005) is cloned into the EcoRV site of pKM2. The MCS1 is able to clone promoter region. The recombinant clone can use for dual-luciferase reporter assay.
Vector backbone pKM2L (Plasmid)
Selectable markers Kan^r
Gene/insert name Renilla reniformis luciferase cDNA
Depositor Yokoyama, Kazunari |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR 1. The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. 2. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
Additional terms and conditions for distribution The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB04026 pKM2L DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Sequence RDB04026z.seq from Depositor
Electronic file Map RDB04026a.gif from Depositor
Electronic file PDF RDB04026.pdf from Depositor

Featured content

Featured content Promoter Collection (Outline) (English text)
Featured content Promoter Collection (Outline) (Japanese text)
Featured content Promoter Collection (Renilla Luciferase) (English text)
Featured content Control Vectors (English text)

References

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB12398_A4G3p1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: M13_-40 RDB12398_A4G3a.seq
>04026_12398_A4G3_M13_(-40)_A01_01.ab1
    1 CCGTCGGCAG CTTGCATGCC TGCAGGTCAT TTAAATAGGG ATAACAGGGT AATGATTAAG
   61 ATCTCGAGCT CTAAGCTTCA CATATGCATG CACTAGTGGC GCCTGTCGAC GCGTAGAATT
  121 CACCCGGGTA CTGCAGAAGT TGGTCGTGAG GCACTGGGCA GGTAAGTATC AAGGTTACAA
  181 GACAGGTTTA AGGAGACCAA TAGAAACTGG GCTTGTCGAG ACAGAGAAGA CTCTTGCGTT
  241 TCTGATAGGC ACCTATTGGT CTTACTGACA TCCACTTTGC CTTTCTCTCC ACAGGTGTCC
  301 ACTCCCAGTT CAATTACAGC TCTTAAGGCT AGAGTACTTA ATACGACTCA CTATAGGCTA
  361 GCCACCATGG CTTCCAAGGT GTACGACCCC GAGCAACGCA AACGCATGAT CACTGGGCCT
  421 CAGTGGTGGG CTCGCTGCAA GCAAATGAAC GTGCTGGACT CCTTCATCAA CTACTATGAT
  481 TCCGAGAAGC ACGCCGAGAA CGCCGTGATT TTTCTGCATG GTAACGCTGC CTCCAGCTAC
  541 CTGTGGAGGC ACGTCGTGCC TCACATCGAG CCCGTGGCTA GATGCATCAT CCCTGATCTG
  601 ATCGGAATGG GTAAGTCCGG CAAGAGCGGG AATGGCTCAT ATCGCCTCCT GGATCACTAC
  661 AAGTACCTCA CCGCTTGGTT CGAGCTGCTG AACCTTCCAA AGAAAATCAT CTTTGTGGGC
  721 CACGACTGGG GGGCTTGTCT GGCCTTTCAC TACTCCTACG AGCACCAAGA CAAGATCAAG
  781 GCCATCGTCC ATGCTGAGAG TGTCGTGGAC GTGATCGAGT CCTGGGACGA GTGGCCTGAC
  841 ATCGAGGAGG ATATCGCCCT GATCAAGAGC GAAGAGGGCG AGAAAATGGT GCTTGAGAAT
  901 AACTTCTTCG TCGAGACCAT GCTCCCAAGC AAGATCATGC GGAAACTGGA GCCTGAGGAG
  961 TTCGCTGCCT ACCCTGGAGC CATTCAAGGG AGAAAGGCGA GGTTAGACGG GCCTACCCTC
 1021 TCCTGGTCCT CGCGAGGATC CCTCTCGTTA GGAGGCCAGG CCCGACGTTC GTCAGATTGA
1081 TCGCGCTACC ACGACTAACC CTTCGGACCA GGCGAACGA
//
Primer: pAxCALNL_F1 RDB12398_A4G3b.seq
>04026_12398_A4G3_pAxCALNLF1_B01_04.ab1
    1 ACCATTTATC TTATCATGTC TGCTCGAAGC GGCCGCTCTA GAATTACTGC TCGTTCTTCA
   61 GCACGCGCTC CACGAAGCTC TTGATGTACT TACCCATTTC ATCTGGAGCG TCCTCCTGGC
  121 TGAAGTGGAG GCCCTTCACC TTCACGAACT CGGTGTTAGG GAACTTCTTA GCTCCCTCGA
  181 CAATAGCGTT GGAAAAGAAC CCAGGGTCGG ACTCGATGAA CATCTTAGGC AGATCGTCGC
  241 TGGCCCGAAG GTAGGCGTTG TAGTTGCGGA CAATCTGGAC GACGTCGGGC TTGCCTCCCT
  301 TAACGAGAGG GATCTCGCGA GGCCAGGAGA GGGTAGGCCG TCTAACCTCG CCCTTCTCCT
  361 TGAATGGCTC CAGGTAGGCA GCGAACTCCT CAGGCTCCAG TTTCCGCATG ATCTTGCTTG
  421 GGAGCATGGT CTCGACGAAG AAGTTATTCT CAAGCACCAT TTTCTCGCCC TCTTCGCTCT
  481 TGATCAGGGC GATATCCTCC TCGATGTCAG GCCACTCGTC CCAGGACTCG ATCACGTCCA
  541 CGACACTCTC AGCATGGACG ATGGCCTTGA TCTTGTCTTG GTGCTCGTAG GAGTAGTGAA
  601 AGGCCAGACA AGCCCCCCAG TCGTGGCCCA CAAAGATGAT TTTCTTTGGA AGGTTCAGCA
  661 GCTCGAACCA AGCGGTGAGG TACTTGTAGT GATCCAGGAG GCGATATGAG CCATTCCCGC
  721 TCTTGCCGGA CTTACCCATT CCGATCAGAT CAGGGATGAT GCATCTAGCC ACGGGCTCGA
  781 TGTGAGGCAC GACGTGCCTC CACAGGTAGC TGGAGGCAGC GTTACCATGC AGAAAAATCA
  841 CGGCGTTCTC GGCGTGCTTC TCGGAATCAT AGTAGTTGAT GAAGGAGTCC AGCACGTTCA
  901 TTTGCTTGCA GCGAGCCCAC CACTGAGGCC CAGTGATCAT GCGTTTGCGT TGCTCGGGGG
  961 TCGTACACCT TGGAAAGCCA TGGTGGCTAG CCTATAGTGA GTCGTATTTA AGTACTCTAG
 1021 CCCTTTAGAG CTGTAATTTG AACTGGAAGT TGACACCTGT TGGAGAGAAG CAAGTGATGG
1081 TCAGGTTAGA CCAATAGGGT GGCCCTATTC CAGGA
//

Please visit Sequencing and PCR primers for primer information.


References

References


2018.12.11

GNP_filter3_RDBDEP_html_181012.pl