Resource data sheet
Prev.

pCMVluc+

Expression vector of firefly luciferase

Catalog number RDB03988
Resource name pCMVluc+
Alternative name pCMV-luc+
Clone info. Espression vector for firefly luc+ driven by CMV promoter. Expression was confirmed by depositor with luciferase.
Vector backbone pCMV (Plasmid)
Selectable markers Amp^r
Gene/insert name firefly (Photinus pyralis) luciferase cDNA
Depositor Hamada, Hirofumi |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
Ordering Please visit Information of Request for Distribution. 
Order form 
[Credit Card Payment]  [Bank Transfer or Check Payment]
Material Transfer Agreement (MTA) [Word]
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB03988 pCMVluc+ DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Featured content

Featured content Control Vectors (English text)

References

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB12358_A4Amp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward RDB12358_A4Ama_2.seq
>03988_12358_A4Am_CMV-Foward_A10_01.ab1
    1 TTGATAAAGC TCCGCCCATG TGACGCAATG GGCGGTAGGC GTGTACGGTG GGAGGTCTAT
   61 ATAAGCAGAG CTCGTTTAGT GAACCGTCAG ATCGCCTGGA GACGCCATCC ACGCTGTTTT
  121 GACCTCCATA GAAGACACCG GGACCGATCC AGCCTCCGGA CTCTAGAGGA TCCGGTACTC
  181 GAGGAACTGA AAAACCAGAA AGTTAACTGG TAAGTTTAGT CTTTTTGTCT TTTATTTCAG
  241 GTCCCGGATC CGGTGGTGGT GCAAATCAAA GAACTGCTCC TCAGTGGATG TTGCCTTTAC
  301 TTCTAGGCCT GTACGGAAGT GTTACTTCTG CTCTAAAAGC TGCGGAATTG TACCCGCGGC
  361 CGCGGGAATT CGATTAGCTT GGCATTCCGG TACTGTTGGT AAAGCCACCA TGGAAGACGC
  421 CAAAAACATA AAGAAAGGCC CGGCGCCATT CTATCCGCTG GAAGATGGAA CCGCTGGAGA
  481 GCAACTGCAT AAGGCTATGA AGAGATACGC CCTGGTTCCT GGAACAATTG CTTTTACAGA
  541 TGCACATATC GAGGTGGACA TCACTTACGC TGAGTACTTC GAAATGTCCG TTCGGTTGGC
  601 AGAAGCTATG AAACGATATG GGCTGAATAC AAATCACAGA ATCGTCGTAT GCAGTGAAAA
  661 CTCTCTTCAA TTCTTTATGC CGGTGTTGGG CGCGTTATTT ATCGGAGTTG CAGTTGCGCC
  721 CGCGAACGAC ATTTATAATG AACGTGAATT GCTCAACAGT ATGGGCATTT CGCAGCCTAC
  781 CGTGGTGTTC GTTTCCAAAA AGGGGTTGCA AAAAATTTTG AACGTGCAAA AAAAGCTCCC
  841 AATCATCCAA AAAATTATTA TCATGGATTC TAAAACGGAT TACCAGGGAT TTCAGTCGAT
  901 GTACACGTTC GTCACATCTC ATCTACCTCC CGGTTTTAAT GAATACGATT TTGTGCCAGA
  961 GTCCTTCGAT AGGGACAAGA CAATTGCACT GATCATGAAC TCCTCTGGAT CTACTGGTCT
 1021 GCCTAAAGGT GTCGCTCTGC CCTCATAGAA CTGCCCTGCG TGAGATTCTC GCATGCCAGA
 1081 GATCCTATTT TTGCATCAAA TCATTTCCGG AATACTGCGA TTTTAAGTGA TGTCATTCAT
1141 TCACGATTTT GGAATGTTTA CCTAACCACC CTCGGGA
//
Primer: Reverse2 RDB12358_A4Amb_2.seq
>03988_12358_A4Am_Reverse2_B02_05.ab1
    1 CTCATGCATT CGCCAGCTTG CATGCCTGCA GGTCGACTCT AGAGGATCCG AAAAAACCTC
   61 CCACACCTCC CCCTGAACCT GAAACATAAA ATGAATGCAA TTGTTGTTGT TAACTTGTTT
  121 ATTGCAGCTT ATAATGGTTA CAAATAAAGC AATAGCATCA CAAATTTCAC AAATAAAGCA
  181 TTTTTTTCAC TGCATTCTAG TTGTGGTTTG TCCAAACTCA TCAATGTATC TTATCATGTC
  241 TGGATCCCCG CGGCCGCGAA TTCACTAGTG ATTCTAGAAT TACACGGCGA TCTTTCCGCC
  301 CTTCTTGGCC TTTATGAGGA TCTCTCTGAT TTTTCTTGCG TCGAGTTTTC CGGTAAGACC
  361 TTTCGGTACT TCGTCCACAA ACACAACTCC TCCGCGCAAC TTTTTCGCGG TTGTTACTTG
  421 ACTGGCGACG TAATCCACGA TCTCTTTTTC CGTCATCGTC TTTCCGTGCT CCAAAACAAC
  481 AACGGCGGCG GGAAGTTCAC CGGCGTCATC GTCGGGAAGA CCTGCGACAC CTGCGTCGAA
  541 GATGTTGGGG TGTTGGAGCA AGATGGATTC CAATTCAGCG GGAGCCACCT GATAGCCTTT
  601 GTACTTAATC AGAGACTTCA GGCGGTCAAC GATGAAGAAG TGTTCGTCTT CGTCCCAGTA
  661 AGCTATGTCT CCAGAATGTA GCCATCCATC CTTGTCAATC AAGGCGTTGG TCGCTTCCGG
  721 ATTGTTTACA TAACCGGACA TAATCATAGG ACCTCTCACA CACAGTTCGC CTCTTTGATT
  781 AACGCCCAGC GTTTTCCCGG TATCCAGATC CACAACCTTC GCTTCAAAAA ATGGAACAAC
  841 TTTACCGACC GCGCCCGGTT TATCATCCCC CCTCGGGTGT AATCAGAATA GCTGATGTAG
  901 TCTCAGTGAG CCCATATCCT TGCCTGATAC CTGGCAGATG GAAACCTCTT GGCAACCGCT
  961 TCCCCGACTT CCTTTAGAGA GGGGAGCGCC ACCAGGAAGC AATTTCGTGT TAAATTAGAT
 1021 AAATCGTATT TGTCATCAGA GTTGCCTTTT TGCGAAGAGA GATAGATGGC ACAGCAGCGC
1081 ACTTTGGAT
//

Please visit Sequencing and PCR primers for primer information.


References

References

user report Nakagomi, M., A novel aromatic carboxylic acid inactivates luciferase by acylation of an enzymatically active regulatory lysine residue. PLoS One, 8 (9): e75445 (2013). PMID 24066181.

2018.12.11

GNP_filter3_RDBDEP_html_181012.pl