Resource data sheet
Prev.

pCMVluc+

Expression vector of firefly luciferase

Catalog number RDB03988
Resource name pCMVluc+
Alternative name pCMV-luc+
Clone info. Espression vector for firefly luc+ driven by CMV promoter. Expression was confirmed by depositor with luciferase.
Vector backbone pCMV (Plasmid)
Selectable markers Amp^r
Gene/insert name firefly (Photinus pyralis) luciferase cDNA
Depositor Hamada, Hirofumi |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
Ordering Please visit Information of Request for Distribution. 
Order form 
[Credit Card Payment]  [Bank Transfer or Check Payment]
Material Transfer Agreement (MTA) [Word]
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB03988 pCMVluc+ DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Control Vectors (English text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB12358_A4Amp1-2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward RDB12358_A4Ama.seq
>03988_12358_A4Am_CMV-Foward_A10_01.ab1
    1 TTGATAAAGC TCCGCCCATG TGACGCAATG GGCGGTAGGC GTGTACGGTG GGAGGTCTAT
   61 ATAAGCAGAG CTCGTTTAGT GAACCGTCAG ATCGCCTGGA GACGCCATCC ACGCTGTTTT
  121 GACCTCCATA GAAGACACCG GGACCGATCC AGCCTCCGGA CTCTAGAGGA TCCGGTACTC
  181 GAGGAACTGA AAAACCAGAA AGTTAACTGG TAAGTTTAGT CTTTTTGTCT TTTATTTCAG
  241 GTCCCGGATC CGGTGGTGGT GCAAATCAAA GAACTGCTCC TCAGTGGATG TTGCCTTTAC
  301 TTCTAGGCCT GTACGGAAGT GTTACTTCTG CTCTAAAAGC TGCGGAATTG TACCCGCGGC
  361 CGCGGGAATT CGATTAGCTT GGCATTCCGG TACTGTTGGT AAAGCCACCA TGGAAGACGC
  421 CAAAAACATA AAGAAAGGCC CGGCGCCATT CTATCCGCTG GAAGATGGAA CCGCTGGAGA
  481 GCAACTGCAT AAGGCTATGA AGAGATACGC CCTGGTTCCT GGAACAATTG CTTTTACAGA
  541 TGCACATATC GAGGTGGACA TCACTTACGC TGAGTACTTC GAAATGTCCG TTCGGTTGGC
  601 AGAAGCTATG AAACGATATG GGCTGAATAC AAATCACAGA ATCGTCGTAT GCAGTGAAAA
  661 CTCTCTTCAA TTCTTTATGC CGGTGTTGGG CGCGTTATTT ATCGGAGTTG CAGTTGCGCC
  721 CGCGAACGAC ATTTATAATG AACGTGAATT GCTCAACAGT ATGGGCATTT CGCAGCCTAC
  781 CGTGGTGTTC GTTTCCAAAA AGGGGTTGCA AAAAATTTTG AACGTGCAAA AAAAGCTCCC
  841 AATCATCCAA AAAATTATTA TCATGGATTC TAAAACGGAT TACCAGGGAT TTCAGTCGAT
  901 GTACACGTTC GTCACATCTC ATCTACCTCC CGGTTTTAAT GAATACGATT TTGTGCCAGA
  961 GTCCTTCGAT AGGGACAAGA CAATTGCACT GATCATGAAC TCCTCTGGAT CTACTGGTCT
 1021 GCCTAAAGGT GTCGCTCTGC CCTCATAGAA CTGCCCTGCG TGAGATTCTC GCATGCCAGA
 1081 GATCCTATTT TTGCATCAAA TCATTTCCGG AATACTGCGA TTTTAAGTGA TGTCATTCAT
1141 TCACGATTTT GGAATGTTTA CCTAACCACC CTCGGGA
//
Primer: Reverse2 RDB12358_A4Amb.seq
>03988_12358_A4Am_Reverse2_B02_05.ab1
    1 CTCATGCATT CGCCAGCTTG CATGCCTGCA GGTCGACTCT AGAGGATCCG AAAAAACCTC
   61 CCACACCTCC CCCTGAACCT GAAACATAAA ATGAATGCAA TTGTTGTTGT TAACTTGTTT
  121 ATTGCAGCTT ATAATGGTTA CAAATAAAGC AATAGCATCA CAAATTTCAC AAATAAAGCA
  181 TTTTTTTCAC TGCATTCTAG TTGTGGTTTG TCCAAACTCA TCAATGTATC TTATCATGTC
  241 TGGATCCCCG CGGCCGCGAA TTCACTAGTG ATTCTAGAAT TACACGGCGA TCTTTCCGCC
  301 CTTCTTGGCC TTTATGAGGA TCTCTCTGAT TTTTCTTGCG TCGAGTTTTC CGGTAAGACC
  361 TTTCGGTACT TCGTCCACAA ACACAACTCC TCCGCGCAAC TTTTTCGCGG TTGTTACTTG
  421 ACTGGCGACG TAATCCACGA TCTCTTTTTC CGTCATCGTC TTTCCGTGCT CCAAAACAAC
  481 AACGGCGGCG GGAAGTTCAC CGGCGTCATC GTCGGGAAGA CCTGCGACAC CTGCGTCGAA
  541 GATGTTGGGG TGTTGGAGCA AGATGGATTC CAATTCAGCG GGAGCCACCT GATAGCCTTT
  601 GTACTTAATC AGAGACTTCA GGCGGTCAAC GATGAAGAAG TGTTCGTCTT CGTCCCAGTA
  661 AGCTATGTCT CCAGAATGTA GCCATCCATC CTTGTCAATC AAGGCGTTGG TCGCTTCCGG
  721 ATTGTTTACA TAACCGGACA TAATCATAGG ACCTCTCACA CACAGTTCGC CTCTTTGATT
  781 AACGCCCAGC GTTTTCCCGG TATCCAGATC CACAACCTTC GCTTCAAAAA ATGGAACAAC
  841 TTTACCGACC GCGCCCGGTT TATCATCCCC CCTCGGGTGT AATCAGAATA GCTGATGTAG
  901 TCTCAGTGAG CCCATATCCT TGCCTGATAC CTGGCAGATG GAAACCTCTT GGCAACCGCT
  961 TCCCCGACTT CCTTTAGAGA GGGGAGCGCC ACCAGGAAGC AATTTCGTGT TAAATTAGAT
 1021 AAATCGTATT TGTCATCAGA GTTGCCTTTT TGCGAAGAGA GATAGATGGC ACAGCAGCGC
1081 ACTTTGGAT
//
Primer: Luc inner_F1 RDB12358_A4Amc.seq
>03988_12358_A9EgF_20_Bp_Luc inner_F1_G07_19_ABI24.ab1
    1 GCTAGATCGA TTTTGTGCCA GAGTCCTTCG ATAGGGACAA GACAATTGCA CTGATCATGA
   61 ACTCCTCTGG ATCTACTGGT CTGCCTAAAG GTGTCGCTCT GCCTCATAGA ACTGCCTGCG
  121 TGAGATTCTC GCATGCCAGA GATCCTATTT TTGGCAATCA AATCATTCCG GATACTGCGA
  181 TTTTAAGTGT TGTTCCATTC CATCACGGTT TTGGAATGTT TACTACACTC GGATATTTGA
  241 TATGTGGATT TCGAGTCGTC TTAATGTATA GATTTGAAGA AGAGCTGTTT CTGAGGAGCC
  301 TTCAGGATTA CAAGATTCAA AGTGCGCTGC TGGTGCCAAC CCTATTCTCC TTCTTCGCCA
  361 AAAGCACTCT GATTGACAAA TACGATTTAT CTAATTTACA CGAAATTGCT TCTGGTGGCG
  421 CTCCCCTCTC TAAGGAAGTC GGGGAAGCGG TTGCCAAGAG GTTCCATCTG CCAGGTATCA
  481 GGCAAGGATA TGGGCTCACT GAGACTACAT CAGCTATTCT GATTACACCC GAGGGGGATG
  541 ATAAACCGGG CGCGGTCGGT AAAGTTGTTC CATTTTTTGA AGCGAAGGTT GTGGATCTGG
  601 ATACCGGGAA AACGCTGGGC GTTAATCAAA GAGGCGAACT GTGTGTGAGA GGTCCTATGA
  661 TTATGTCCGG TTATGTAAAC AATCCGGAAG CGACCAACGC CTTGATTGAC AAGGATGGAT
  721 GGCTACATTC TGGAGACATA GCTTACTGGG ACGAAGACGA ACACTTCTTC ATCGTTGACC
  781 GCCTGAAGTC TCTGATTAAG TACAAAGGCT ATCAGGTGGC TCCCGCTGAA TTGGAATCCA
  841 TCTTGCTCCA ACACCCCAAC ATCTTCGACG CACGTGTCGC ACGTCTTCCC GACGATGACG
  901 CCGGTGAACT TCCCGCCGCC GTTGTTGTTT TGGAGCACGG GAAGACGATG ACGGAAAAAG
  961 AGATCGTGGA TTACGTCGCC CAGTCAAGTA ACACGCGAAA AAGTTGCGCG AAGAAGTGTG
 1021 TTGTGGAACG AAGTACGGAA ACGTCCTAAC GGAAACTCGA CGCAGCAAAA TTCAGAGAGA
1081 TTCCCTCCTA TTAAAGGTC
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

user report Nakagomi, M., A novel aromatic carboxylic acid inactivates luciferase by acylation of an enzymatically active regulatory lysine residue. PLoS One, 8 (9): e75445 (2013). PMID 24066181.

2019.05.30

GNP_filter3_RDBDEP_html_190430.pl