RDB03958 - pCAhIL12

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

pCAhIL12 (#RDB03958)

Expression vector of human IL-12 (p40 and p35)

Alternative name	pCAcchIL12
Clone info.	Expression vector of human IL-12 (p40 and p35) driven by CA promoter. Regarding hIL12p40, cDNA fragment 43nt to 1029nt of NM_002187.2 is cloned. Regarding hIL12p35, cDNA fragment 170nt to 829nt of NM_000882.1 is cloned. However, this fragment corresponds 324nt to 983nt of recent version of the RefSeq (NM_000882.3, CDS = 222nt to 983nt).
Comment	Expression was confirmed with ELISA by depositor. Known differences (at least): C to G substitution at 381nt of NM_002187.2 (Ser, synonym). T to C substitution at 961nt of NM_000882.1 (Met to Thr).
Vector backbone	pCA (Plasmid)
Selectable markers	Amp^r
Gene/insert name	Human IL-12 p35 cDNA Human IL-12 p40 cDNA
Depositor\|Developer	Hamada, Hirofumi \|

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link]

Terms and conditions for distribution The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
Additional terms and conditions:
Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.

提供案内 (日本国内) [open/close]

Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link]
Terms and conditions for distribution	The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. Additional terms and conditions: Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件	非営利機関の非営利学術研究へ提供する。利用者が本件リソースを利用した研究成果に基づき特許申請、商業活動等を行う場合は、利用者は事前に寄託者に連絡する。利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する。利用者は、研究成果の公表にあたって謝辞の表明を必要とする。 MTAに書く付加的使用条件： CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用し、大阪大学宮崎純一博士への謝辞の表明を必要とする。

必要書類

提供依頼書

[依頼書の記入例

]
提供同意書 (MTA, 非営利学術目的用)[Word

] [MTAの記入例

]
手続きの概要は、「提供申込みについて[link]」をご覧ください。

MTAに書く使用条件

非営利機関の非営利学術研究へ提供する。利用者が本件リソースを利用した研究成果に基づき特許申請、商業活動等を行う場合は、利用者は事前に寄託者に連絡する。利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する。利用者は、研究成果の公表にあたって謝辞の表明を必要とする。
MTAに書く付加的使用条件：
CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用し、大阪大学宮崎純一博士への謝辞の表明を必要とする。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB03958	pCAhIL12	DNA solution	JPY 9,460 (not-for-profit academic purpose) plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pCAhIL12 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB03958).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB03958_A8Ahp1-2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pCAG-F_V2 (Pr0330) Region: CAG pro,insert 5'(hIL12p40 5') Sequence file: RDB03958_A8Ahd.seq
>03958_03958_A8Ah_pCAG-F_V2_A01_01_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNTG NGCGGNNCCG ANTCTGGGNN GCGCCGCCGC ACCCCCTCTA 61 GCGGGCGCGG GGCNANNNNN NNCGGCGCCG GCAGGAAGGA AATGGGCGGG GAGGGCCTTC 121 GTGCGTCGCC GCGCCGCCGT CCCCTTCTCC CTCTCCAGCC TCGGGGCTGT CCGCGGGGGG 181 ACGGCTGCCT TCGGGGGGGA CGGGGCAGGG CGGGGTTCGG CTTCTGGCGT GTGACCGGCG 241 GCTCTAGAGC CTCTGCTAAC CATGTTCATG CCTTCTTCTT TTTCCTACAG CTCCTGGGCA 301 ACGTGCTGGT TATTGTGCTG TCTCATCATT TTGGCAAAGA ATTCTAGACT GCCATGTGTC 361 ACCAGCAGTT GGTCATCTCT TGGTTTTCCC TGGTTTTTCT GGCATCTCCC CTCGTGGCCA 421 TATGGGAACT GAAGAAAGAT GTTTATGTCG TAGAATTGGA TTGGTATCCG GATGCCCCTG 481 GAGAAATGGT GGTCCTCACC TGTGACACCC CTGAAGAAGA TGGTATCACC TGGACCTTGG 541 ACCAGAGCAG TGAGGTCTTA GGCTCTGGCA AAACCCTGAC CATCCAAGTC AAAGAGTTTG 601 GAGATGCTGG CCAGTACACC TGTCACAAAG GAGGCGAGGT TCTAAGCCAT TCGCTCCTGC 661 TGCTTCACAA AAAGGAAGAT GGAATTTGGT CGACTGATAT TTTAAAGGAN CAGAAAGAAC 721 CCAAAAATAA GANCTTTCTA AGANGCGAGG CCNAGAATTA TTCTGGACGT TTCACCTGCT 781 GGTGGCTGAC GACAATCAGT ACTGATTTGA CATTCAGTGT CNAAAGCAGC AGANGCTCTT 841 CTGANCCCNN AGGGGTGANG TGCGGANCTG CTACACTCTC TGCAGANAGA NTCANANGGN 901 ANAACAANGA GTATGANTAC TCANNGGNNT GNCNNGANGA NAGTGNCTGC CCAGCTGNTG 961 ANGANANTCT GNCCNTTGAN GNNNTNNNNG NNGNCNTTCN NNAGCTNNNG TATNAAAACT 1021 ACNNNNNCNN CTTCTTCNTN ANGNNNNNCA TNNNNNTNNN NNNNNNN //
Primer: pBAC_lac_R (Pr0545) Region: insert 3'(beta-globin pA) Sequence file: RDB03958_A8Ahe.seq
>03958_03958_B2LlF_pBAC_lac_R_C01_03_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNNNTTGG GCTGCAGGTC GAGGGNNCTC CATAAGAGAA 61 GAGGGACAGC TATGACTGGG AGTAGTCAGG AGAGGAGGAA AAATCTGGCT AGTAAAACAT 121 GTAAGGAAAA TTTTAGGGAT GTTAAAGAAA AAAATAACAC AAAACAAAAT ATAAAAAAAA 181 TCTAACCTCA AGTCAAGGCT TTTCTATGGA ATAAGGAATG GACAGCAGGG GGCTGTTTCA 241 TATACTGATG ACCTCTTTAT AGCCAACCTT TGTTCATGGC AGCCAGCATA TGGGCATATG 301 TTGCCAAACT CTAAACCAAA TACTCATTCT GATGTTTTAA ATGATTTGCC CTCCCATATG 361 TCCTTCCGAG TGAGAGACAC AAAAAATTCC AACACACTAT TGCAATGAAA ATAAATTTCC 421 TTTATTANCC ANAAGTCANA TGCTCAAGGG GCTTCATGAT GTCCCCNNNN TTTTTGGCAG 481 AGNNAANAAG ATCCTTANGA ANNNNTCNNA NNNNNCNNNN NNCTATCNAT AGNCACTGCC 541 CGAATTCNNA AAGCATGAAN ANNNNNGNNN ANNTTGATTN TNANNNNNAN NNNNNNNNN //
Primer: RDB03958#1 (Pr0535) Region: insert mid(hIL12p40 3') Sequence file: RDB03958_A8Ahf.seq
>03958_03958_A8Ah_RDB03958#1_A07_01_ABI24.ab1 1 NNNNNNNNNN NNNNNGNNNN NGNNNATCNG TACTGATTTG ACNTTCNGTG TCAAAAGCAG 61 CAGAGGCTCT TCTGACCCCC AAGGGGTGAC GTGCGGAGCT GCTACACTCT CTGCAGAGAG 121 AGTCAGAGGG GACAACAAGG AGTATGAGTA CTCAGTGGAG TGCCAGGAGG ACAGTGCCTG 181 CCCAGCTGCT GAGGAGAGTC TGCCCATTGA GGTCATGGTG GATGCCGTTC ACAAGCTCAA 241 GTATGAAAAC TACACCAGCA GCTTCTTCAT CAGGGACATC ATCAAACCTG ACCCACCCAA 301 GAACTTGCAG CTGAAGCCAT TAAAGAATTC TCGGCAGGTG GAGGTCAGCT GGGAGTACCC 361 TGACACCTGG AGTACTCCAC ATTCCTACTT CTCCCTGACA TTCTGCGTTC AGGTCCAGGG 421 CAAGAGCAAG AGAGAAAAGA AAGATAGAGT CTTCACGGAC AAGACCTCAG CCACGGTCAT 481 CTGCCGCAAA AATGCCAGCA TTAGCGTGCG GGCCCAGGAC CGCTACTATA GCTCATCTTG 541 GAGCGAATGG GCATCTGTGC CCTGCAGTTA GGATCGATCC TCTAGAGAAT TCCGCCCCCC 601 CNNNNNNNCC NNNNNNNNCT CCCCNNNNCC NAACGTTACT GGCCGAAGCC GCTTGGANTA 661 AGGNCGGNGT GCGTTTGTCT ATATGNNATT TTCCNCCANA TTGCCNNCNT TTGGNNNTGT 721 GAGGGCCCGN NANNCTGNCC CTGTCTTCTT GACNANCNTT CCTANGGGTC TTTCCCCTCT 781 CGCNAAAGGA ATGNNANGNT CTGNNNAATG NNNNNNANNA ANNANNNNNN NNNN //
Primer: IRES-F (Pr0036) Region: insert mid(hIL12p35) Sequence file: RDB03958_A8Ahg.seq
>03958_03958_A8Ah_IRES-F_D01_04_ABI08.ab1 1 NNNNNNNNNN NNNNNNNTTN NGTCGANGTT ANNANACGTC TAGGCCCCCC GAACCACGGG 61 GACGTGGTTT TCNNNNNNNN NNNNNNATGA TAAGCTTGCC ACGATCCGGC TCTAGACTCA 121 NNATGTGTCC AGCGCGCAGC CTCCTCCTTG TGGCTACCCT GGTCCTCCTG GACCACCTCA 181 GTTTGGCCAG AAACCTCCCC GTGGCCACTC CAGACCCAGG AATGTTCCCA TGCCTTCACC 241 ACTCCCAAAA CCTGCTGAGG GCCGTCAGCA ACATGCTCCA GAAGGCCAGA CAAACTCTAG 301 AATTTTACCC TTGCACTTCT GAAGAGATTG ATCATGAAGA TATCACAAAA GATAAAACCA 361 GCACAGTGGA GGCCTGTTTA CCATTGGAAT TAACCAAGAA TGAGAGTTGC CTAAATTCCA 421 GAGAGACCTC TTTCATAACT AATGGGAGTT GCCTGGCCTC CAGAAAGACC TCTTTTATGA 481 TGGCCCTGTG CCTTAGTAGT ATTTATGAAG ACTTGAAGAT GTACCAGGTG GAGTTCAAGA 541 CCATGAATGC AAAGCTTCTG ATGGATCCTA AGAGGCAGAT CTTTCTAGAT CAAAACATGC 601 TGGCAGTTAT TGATGAGCTG ATGCAGGCCC TGAATTTCAA CAGTGAGACT GTGCCACAAA 661 AATCCTCCCT TGAAGAACCG GATTTTTATA AAACTAAAAT CAAGCTCTGC ATACTTCTTC 721 ATGCTTTCAG AATTCGGGCA GTGACTATTG ATAGAGTGAC GAGCTATCTG AATGCTTCCT 781 AAGGATCTTT TTCCCTCTGC CNAAAATTAT GGGGACATCA TGAAGCCCCT TGANCATCTG 841 ACTTCTGGCT AATAAAGGAA ATTTATTTTC NTTGCAATAG TGTGNTGGAN NTTTTTGNGT 901 CTCTCACTCG GAAGGANATA TGGGAGGGCA AATCATTTNA AACATCANAA NGAGTANTTG 961 GTTTANAGTT NNNCANCATA TGCCNNTATG CTGGCTGNCN TGNNNNANNN NNNNCTATNA 1021 NNANNTCNTC AGNATNNNNN NNNNCCCNNN NTNNNNNNNN NN //
Primer: SV40pro_ori_F (Pr0731) Region: SV40 pro_ori,SV40 pA Sequence file: RDB03958_A8Ahh.seq
>03958_03958_B2LlF_SV40pro_ori_F_D01_04_ABI08.ab1 1 NNNNNNNNNN NNNNNGGAGG CTTTTTTGGA GGCCTAGGCT TTTGCAAAAA GCTAACTTGT 61 TTATTGCAGC TTATAATGGT TACAAATAAA GCAATAGCAT CACAAATTTC ACAAATAAAG 121 CATTTTTTTC ACTGCATTCT AGTTGTGGTT TGTCCAAACT CATCAATGTA TCTTATCATG 181 TCTGGATCCG CTGCATTAAT GAATCGGCCA ACGCGCGGGG AGAGGCGGTT TGCGTATTGG 241 GCGCTCTTCC GCTTCCTCGC TCACTGACTC GCTGCGCTCG GTCGTTCGGC TGCGGCGAGC 301 GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG 361 AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGNAAAAAGG CCGCGTTGCT 421 GGCGTTTTTC CATANGCTCC GCCCCCCTGA NNAGCATNNN NNNAATNNNN NNNCAANNNN 481 NANGNGGNNA AACCCNNNNN NNNNN //