Please review the QC test results indicated by icon below as well as clone information before placing your order.

pWEAx-FRGD

Cosmid vector for F/RGD fiber mutant Adv construction

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.(Hum. Gene Ther., 13, 613-626 (2002); Mol. Ther., 7, 354-365 (2003).) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
Ordering	Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
Remarks	Remember that you will be working with samples containing infectious virus.

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提供条件	非営利機関の非営利学術研究へ提供する。利用者が本件リソースを利用した研究成果に基づき特許申請、商業活動等を行う場合は、利用者は事前に寄託者に連絡する。利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Hum. Gene Ther., 13, 613-626 (2002); Mol. Ther., 7, 354-365 (2003).)。利用者は、研究成果の公表にあたって謝辞の表明を必要とする。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
備考	このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

How to cite this biological resource

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB03952_A9Fap1-2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxcwit2-Fw (Pr0134) Region: Ad5 psi,ITR(truncated),AmpR pro Sequence file: RDB03952_A9Faa.seq
>03952_03952_A9Fa_1_pAxcwit2_Fw_E05_14_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNCNA GTGNNCGGGC CTCAGCTCCT CGGTCACATC    61 CAGCATCACA GGCTGGTTCC TAATATGTTT ACCGCCACAC TCGCAGGGTC TGCACCTGGT   121 GCGGGTCTCA TCGTACCTCA GCACCTTCCA GATCCATCGT CGCGAGTCGA CAATCGATTT   181 AAATCGATGC TGACTATAAT AATAAAACGC CAACTTTGAC CCGGAACGCG GAAAACACCT   241 GAGAAAAACA CCTGGGCGAG TCTCCACGTA AACGGTCAAA GTCCCCGCGG CCCTAGACAA   301 ATATTACGCG CTATGAGTAA CACAAAATTA TTCAGATTTC ACTTCCTCTT ATTCAGTTTT   361 CCCGCGAAAA TGGCCAAATC TTACTCGGTT ACGCCCAAAT TTACTACAAC ATCCGCCTAA   421 AACCGCGCGA AAATTGTCAC TTCCTGTGTA CACCGGCGCA CACCAAAAAC GTCACTTTTG   481 CCACATCCGT CGCTTACATG TGTTCCGCCA CACTTGCAAC ATCACACTTC CGCCACACTA   541 CTACGTCACC CGCCCCGTTC CCACGCCCCG CGCCACGTCA CAAACTCCAC CCCCTCATTA   601 TCATATTGGC CCGGATCCGG AATTCTTGAA GACGAAAGGG CCTCGTGATA CGCCTATTTT   661 TATAGGTTAA TGTCATGATA ATAATGGTTT CTTAGACGTC AGGTGGCACT TTTCGGGGAA   721 ATGTGCGCGG AACCCCTATT TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA   781 TGAGACAATA ACCCTGATAA ATGCTTCAAT AATATTGAAA AAGGAAGAGT ATGAGTATTC   841 AACATTTCCG TGTCGCCCTT ATTCCCTTTT TTTGCGGCAT TTTGCCTTCC TGTTTTTGCT   901 CACCNAGAAA CGCTGGTGAA AGTAAAAGAN GCTGAAGNTC AGTTNGGNGC ACNAGNGGGN   961 TNNATCNAAC TGGNTNTCNN NNNCGGTNNN ATNCNTTNNN ANNTTTCGNC NNNNANNNNN 1021 NNTTTNNNNN NNNNN //
Primer: fiber-M_F2 (Pr0638) Region: Fiber-RGD Sequence file: RDB03952_A9Fab.seq
>03952_03952_A9Fa_1_fiber-M_F2_C02_08_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNG NNNNGCCTAT ACNAACGCTG TTGGNNTTAT GCCTAACCTA    61 TCAGCTTATC CAAAATCTCA CGGTAAAACT GCCAAAAGTA ACATTGTCAG TCAAGTTTAC   121 TTAAACGGAG ACAAAACTAA ACCTGTAACA CTAACCATTA CACTAAACGG TACACAGGAA   181 ACAGGAGACA CAACTTGTGA CTGCCGCGGA GACTGTTTCT GCCCAAGTGC ATACTCTATG   241 TCATTTTCAT GGGACTGGTC TGGCCACAAC TACATTAATG AAATATTTGC CACCTCGAGT   301 TACACTTTTT CATACATTGC CCAAGAATAA GGATCCACGC GTGTCGACAA GAATAAAGAA   361 TCGTTTGTGT TATGTTTCAA CGTGTTTATT TTTCAATTGC AGAAAATTTC AAGTCATTTT   421 TCATTCAGTA GTATAGCCCC ACCACCACAT AGCTTATACA GATCACCGTA CCTTAATCAA   481 ACTCACAGAA CCCTAGTATT CAACCTGCCA CCTCCCTCCC AACACACAGA GTACACAGTC   541 CTTTCTCCCC GGCTGGCCTT AAAAAGCATC ATATCATGGG TAACAGACAT ATTCTTAGGT   601 GTTATATTCC ACACGGTTTC CTGTCGAGCC AAACGCTCAT CAGTGATATT AATAAACTCC   661 CCGGGCAGCT CACTTAAGTT CATGTCGCTG TCCAGCTGCT GAGCCACAGG CTGCTGTCCA   721 ACTTGCGGTT GCTTAACGGG CGGCGAANGA GAAGTCCACG CCTACATGGG GGTAGAGTCA   781 TAATCGTGCA TCANGATAGG GCGGTGGTGC TGCAGCAGCG CGCGAATAAA CTGCTGCCGC   841 CGCCGCTCCG TCCTGCANGA ATACAACATG GCAGTGGTCT CCTCANCGAT GANTCGCACC   901 GCCCGCANCA TAAGGNGNNN TGNCCTNCGG GCACANCAGC NCACCCTGAT NTCACNTNAA   961 NTCAGCNCAG NAACNGCNNN NCANNNNNNN NNTNTTGTTC NAANNCCNNN ANNNNNNNNN 1021 N //
Primer: Neo-M_F (Pr0147) Region: NeoR_KanR Sequence file: RDB03952_A9Fac.seq
>03952_03952_B3B1F_1_Neo-M_F_E01_13_ABI24.ab1     1 NNNNNNNNNN NNANGGCTGN NGCNNNGCGG CGGCTGCATA CGCTTGATCC GGCTACCTGC    61 CCATTCGACC ACCAAGCGAA ACATCGCATC GAGCGAGCAC GTACTCGGAT GGAAGCCGGT   121 CTTGTCGATC AGGATGATCT GGACGAAGAG CATCAGGGGC TCGCGCCAGC CGAACTGTTC   181 GCCAGGCTCA AGGCGCGCAT GCCCGACGGC GAGGATCTCG TCGTGACCCA TGGCGATGCC   241 TGCTTGCCGA ATATCATGGT GGAAAATGGC CGCTTTTCTG GATTCATCGA CTGTGGCCGG   301 CTGGGTGTGG CGGACCGCTA TCAGGACATA GCGTTGGCTA CCCGTGATAT TGCTGAAGAG   361 CTTGGCGGCG AATGGGCTGA CCGCTTCCTC GTGCTTTACG GTATCGCCGC TCCCGATTCG   421 CAGCGCATCG CCTTCTATCG CCTTCTTGAC GAGTTCTTCT GAGCGGGACT CTGGGGTTCG   481 AAATGACCGA CCAAGCGACG CCCAACCTGC CATCACGAGA TTTCGATTCC ACCGCCGCCT   541 TCTATGAAAG GTTGGGCTTC GGAATCGTTT TCCGGGACGC CGGCTGGATG ATCCTCCAGC   601 GCGGGGATCT CATGCTGGAG TTCTTCGCCC ACCCCGGGCT CGATCCCCTC GCGAGTTGGT   661 TCAGCTGCTG CCTGANGCTG GACGACCTCG CGGAGTTCTA CCGGCAGTGC AAATCCGTCN   721 GCATCCAGGA AACCAGCAGC GGCTATCCGC GCATCCATGC CCCCGAACTG CANGAGTGGN   781 GAGGCACGAT GGCCGCTTTG GNCCGGATCT TTGTGAAGGA NCCTTACTTC TGTGGTGTGA   841 CATANTTGGA CAAACTACCT ACNGANNNTT AAAGCTCTNA GGNAAATANA AAATTTTNAA   901 GTGNATNATG TGTTAAACTA CNGATTCTNN TTGNTTGTGT ATTTTANANT NNNNCNNANN   961 NNNNTGNNGN ANGGNNNCNG NNNNNGNNNN NNNNTNNNNN NNNAAANNNN NNTNNNNNNN 1021 NNN //
Primer: Neo-B_R (Pr0911) Region: NeoR_KanR,SV40 pro_ori Sequence file: RDB03952_A9Fad.seq
>03952_03952_B3B1F_1_Neo-B_R_F01_16_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNTGCGT GCNATCNNTC TTGTTCNATC ATGCGAAACG    61 ATCCTCATCC TGTCTCTTGA TCAGATCTTG ATCCCCTGCG CCATCAGATC CTTGGCGGCA   121 AGAAAGCCAT CCAGTTTACT TTGCAGGGCT TCCCAACCTT ACCAGAGGGC GCCCCAGCTG   181 GCAATTCCGG TTCGCTTGCT GTCCATAAAA CCGCCCAGTC TAGCTATCGC CATGTAAGCC   241 CACTGCAAGC TACCTGCTTT CTCTTTGCGC TTGCGTTTTC CCTTGTCCAG ATAGCCCAGT   301 AGCTGACATT CATCCGGGGT CAGCACCGTT TCTGCGGACT GGCTTTCTAC GTGTTCCGCT   361 TCCTTTAGCA GCCCTTGCGC CCTGAGTGCT TGCGGCAGCG TGAAGCTTTT TGCAAAAGCC   421 TAGGCCTCCA AAAAAGCCTC CTCACTACTT CTGGAATAGC TCAGAGGCCG AGGCGGCCTC   481 GGCCTCTGCA TAAATAAAAA AAATTAGTCA GCCATGGGGC GGAGAATGGG CGGAACTGGG   541 CGGAGTTAGG GGCGGGATGG GCGGAGTTAG GGGCGGGACT ATGGTTGCTG ACTAATTGAG   601 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCTGGTTGC   661 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC   721 ACACCCTAAC TGACACACAT TCCACAGCCG GATCTGCAGG ANCCAACGCT GCCCGAGANG   781 CGCCGCGTGC GGCTGCTGGA NATGGCGGAN GCGATGGATA TGTTCTGCCN AGGGTTGGTT   841 TGCGCATTCA CAGTTCTCCG CAAGAANTGA TTGGCTNCNA TTCTTGGANT GGTGAATNCG   901 TTANCGAGGN GCCGCCNGCT TCCNTNCNNN CGAGGNGGNC CGGCNNCNNN NNNNNNGNNG 961 CNNNNCGGGG NNNCNNANNN NNNNNANGGN NGNNNNNTAN NNNNNNNNN //
Primer: AxIT_R (Pr0347) Region: ITR,cos 3' Sequence file: RDB03952_A9Fae.seq
>03952_03952_A9Fa_1_AxIT_R_F05_17_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NAAGCCNNAA AACCCACAAC TTCCTCAAAT CGTCACTTCC    61 GTTTTCCCAC GTTACGTCAC TTCCCNTTTT AAGAAAACTA CAATTCCCAA CACATACAAG   121 TTACTCCGCC CTAAAACCTA CGTCACCCGC CCCGTTCCCA CGCCCCGCGC CACGTCACAA   181 ACTCCACCCC CTCATTATCA TATTGGCTTC AATCCAAAAT AAGGTATATT ATTGATGATG   241 AAGCTTGGTT AATTAACGTT AATTAACCGG CGGCCGCCAC CGCTTCGATA AGCTTTAATG   301 CGGTAGTTTA TCACAGTTAA ATTGCTAACG CAGTCAGGCA CCGTGTATGA AATCTAACAA   361 TGCGCTCATC GTCATCCTCG GCACCGTCAC CCTGGATGCT GTAGGCATAG GCTTGGTTAT   421 GCCGGTACTG CCGGGCCTCT TGCGGGATAT CGTCCATTCC GACAGCATCG CCAGTCACTA   481 TGGCGTGCTG CTAGCGCTAT ATGCGTTGAT GCAATTTCTA TGCGCACCCG TTCTCGGAGC   541 ACTGTCCGAC CGCTTTGGCC GCCGCCCAGT CCTGCTCGCT TCGCTACTTG GAGCCACTAT   601 CGACTACGCG ATCATGGCGA CCACACCCGT CCTGTGGATC TGCCTCGCTG GCCTGCCGCA   661 GTTCTTCAAC CTCCCGGCGC AGCTTTTCGT TCTCAATTTC AGCATCCCTT TCGGCATACC   721 ATTTTATGAC GGCGGCAGAG TCATAAAGCA CCTCATTACC CTTGCCACCG CCTCGCAGAA   781 CGGGCATTCC CTGTTCCTGC CAGTTCTGAA TGGTANGGAT ACTCGCACCG AAAATGTCAG   841 CCAGCTGCTT TTTGTTGANT TCCATTGTNC ANTCCACGGN NNAAAACAGA GAAAGGAANN   901 GACAGANGCC AAAAAGCTCG CTTTCAGCAC CNGTCGTTTC CTTTCNTTTN ANANGGNATT 961 TTNAATNAAA ACNTTAANTT ATGANGANNN NNNNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.05.01

GNP_filter3_RDBDEP_html_230501.pl