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pWEAx-FRGD (#RDB03952)

Cosmid vector for F/RGD fiber mutant Adv construction

Clone info. Shuttle vector to make recombinant adenovirus with chimeric fiber F/RGD.
Vector backbone pWE15 (Cosmid, use packaging extracts for transforming E. coli host)
Size of vector backbone 39.4 kb
Selectable markers Amp^r
Gene/insert name AdV_5 whole genome Genomic DNA
Depositor|Developer Hamada, Hirofumi |
 

Distribution information

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Terms and conditions for distribution The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.(Hum. Gene Ther., 13, 613-626 (2002); Mol. Ther., 7, 354-365 (2003).) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
Remarks Remember that you will be working with samples containing infectious virus.
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提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 非営利機関の非営利学術研究へ提供する。利用者が本件リソースを利用した研究成果に基づき特許申請、商業活動等を行う場合は、利用者は事前に寄託者に連絡する。利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Hum. Gene Ther., 13, 613-626 (2002); Mol. Ther., 7, 354-365 (2003).)。利用者は、研究成果の公表にあたって謝辞の表明を必要とする。
備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB03952 pWEAx-FRGD DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pWEAx-FRGD was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB03952).

Reference section:

Tsuda, H., Wada, T., Ito, Y., Uchida, H., Dehari, H., Nakamura, K., Sasaki, K., Kobune, M., Yamashita, T., Hamada, H., Efficient BMP2 gene transfer and bone formation of mesenchymal stem cells by a fiber-mutant adenoviral vector. Mol. Ther., 7, 354-365 (2003). PMID 12668131.
Nakamura, T., Sato, K., Hamada, H., Effective gene transfer to human melanomas via integrin-targeted adenoviral vectors. Hum. Gene Ther., 13, 613-626 (2002). PMID 11916485.

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB03952_A9Fap1-2.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxcwit2-Fw (Pr0134)
Region: Ad5 psi,ITR(truncated),AmpR pro
Sequence file: RDB03952_A9Faa.seq check
>03952_03952_A9Fa_1_pAxcwit2_Fw_E05_14_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNCNA GTGNNCGGGC CTCAGCTCCT CGGTCACATC
   61 CAGCATCACA GGCTGGTTCC TAATATGTTT ACCGCCACAC TCGCAGGGTC TGCACCTGGT
  121 GCGGGTCTCA TCGTACCTCA GCACCTTCCA GATCCATCGT CGCGAGTCGA CAATCGATTT
  181 AAATCGATGC TGACTATAAT AATAAAACGC CAACTTTGAC CCGGAACGCG GAAAACACCT
  241 GAGAAAAACA CCTGGGCGAG TCTCCACGTA AACGGTCAAA GTCCCCGCGG CCCTAGACAA
  301 ATATTACGCG CTATGAGTAA CACAAAATTA TTCAGATTTC ACTTCCTCTT ATTCAGTTTT
  361 CCCGCGAAAA TGGCCAAATC TTACTCGGTT ACGCCCAAAT TTACTACAAC ATCCGCCTAA
  421 AACCGCGCGA AAATTGTCAC TTCCTGTGTA CACCGGCGCA CACCAAAAAC GTCACTTTTG
  481 CCACATCCGT CGCTTACATG TGTTCCGCCA CACTTGCAAC ATCACACTTC CGCCACACTA
  541 CTACGTCACC CGCCCCGTTC CCACGCCCCG CGCCACGTCA CAAACTCCAC CCCCTCATTA
  601 TCATATTGGC CCGGATCCGG AATTCTTGAA GACGAAAGGG CCTCGTGATA CGCCTATTTT
  661 TATAGGTTAA TGTCATGATA ATAATGGTTT CTTAGACGTC AGGTGGCACT TTTCGGGGAA
  721 ATGTGCGCGG AACCCCTATT TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA
  781 TGAGACAATA ACCCTGATAA ATGCTTCAAT AATATTGAAA AAGGAAGAGT ATGAGTATTC
  841 AACATTTCCG TGTCGCCCTT ATTCCCTTTT TTTGCGGCAT TTTGCCTTCC TGTTTTTGCT
  901 CACCNAGAAA CGCTGGTGAA AGTAAAAGAN GCTGAAGNTC AGTTNGGNGC ACNAGNGGGN
  961 TNNATCNAAC TGGNTNTCNN NNNCGGTNNN ATNCNTTNNN ANNTTTCGNC NNNNANNNNN
1021 NNTTTNNNNN NNNNN
//
Primer: fiber-M_F2 (Pr0638)
Region: Fiber-RGD
Sequence file: RDB03952_A9Fab.seq check
>03952_03952_A9Fa_1_fiber-M_F2_C02_08_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNG NNNNGCCTAT ACNAACGCTG TTGGNNTTAT GCCTAACCTA
   61 TCAGCTTATC CAAAATCTCA CGGTAAAACT GCCAAAAGTA ACATTGTCAG TCAAGTTTAC
  121 TTAAACGGAG ACAAAACTAA ACCTGTAACA CTAACCATTA CACTAAACGG TACACAGGAA
  181 ACAGGAGACA CAACTTGTGA CTGCCGCGGA GACTGTTTCT GCCCAAGTGC ATACTCTATG
  241 TCATTTTCAT GGGACTGGTC TGGCCACAAC TACATTAATG AAATATTTGC CACCTCGAGT
  301 TACACTTTTT CATACATTGC CCAAGAATAA GGATCCACGC GTGTCGACAA GAATAAAGAA
  361 TCGTTTGTGT TATGTTTCAA CGTGTTTATT TTTCAATTGC AGAAAATTTC AAGTCATTTT
  421 TCATTCAGTA GTATAGCCCC ACCACCACAT AGCTTATACA GATCACCGTA CCTTAATCAA
  481 ACTCACAGAA CCCTAGTATT CAACCTGCCA CCTCCCTCCC AACACACAGA GTACACAGTC
  541 CTTTCTCCCC GGCTGGCCTT AAAAAGCATC ATATCATGGG TAACAGACAT ATTCTTAGGT
  601 GTTATATTCC ACACGGTTTC CTGTCGAGCC AAACGCTCAT CAGTGATATT AATAAACTCC
  661 CCGGGCAGCT CACTTAAGTT CATGTCGCTG TCCAGCTGCT GAGCCACAGG CTGCTGTCCA
  721 ACTTGCGGTT GCTTAACGGG CGGCGAANGA GAAGTCCACG CCTACATGGG GGTAGAGTCA
  781 TAATCGTGCA TCANGATAGG GCGGTGGTGC TGCAGCAGCG CGCGAATAAA CTGCTGCCGC
  841 CGCCGCTCCG TCCTGCANGA ATACAACATG GCAGTGGTCT CCTCANCGAT GANTCGCACC
  901 GCCCGCANCA TAAGGNGNNN TGNCCTNCGG GCACANCAGC NCACCCTGAT NTCACNTNAA
  961 NTCAGCNCAG NAACNGCNNN NCANNNNNNN NNTNTTGTTC NAANNCCNNN ANNNNNNNNN
1021 N
//
Primer: Neo-M_F (Pr0147)
Region: NeoR_KanR
Sequence file: RDB03952_A9Fac.seq check
>03952_03952_B3B1F_1_Neo-M_F_E01_13_ABI24.ab1
    1 NNNNNNNNNN NNANGGCTGN NGCNNNGCGG CGGCTGCATA CGCTTGATCC GGCTACCTGC
   61 CCATTCGACC ACCAAGCGAA ACATCGCATC GAGCGAGCAC GTACTCGGAT GGAAGCCGGT
  121 CTTGTCGATC AGGATGATCT GGACGAAGAG CATCAGGGGC TCGCGCCAGC CGAACTGTTC
  181 GCCAGGCTCA AGGCGCGCAT GCCCGACGGC GAGGATCTCG TCGTGACCCA TGGCGATGCC
  241 TGCTTGCCGA ATATCATGGT GGAAAATGGC CGCTTTTCTG GATTCATCGA CTGTGGCCGG
  301 CTGGGTGTGG CGGACCGCTA TCAGGACATA GCGTTGGCTA CCCGTGATAT TGCTGAAGAG
  361 CTTGGCGGCG AATGGGCTGA CCGCTTCCTC GTGCTTTACG GTATCGCCGC TCCCGATTCG
  421 CAGCGCATCG CCTTCTATCG CCTTCTTGAC GAGTTCTTCT GAGCGGGACT CTGGGGTTCG
  481 AAATGACCGA CCAAGCGACG CCCAACCTGC CATCACGAGA TTTCGATTCC ACCGCCGCCT
  541 TCTATGAAAG GTTGGGCTTC GGAATCGTTT TCCGGGACGC CGGCTGGATG ATCCTCCAGC
  601 GCGGGGATCT CATGCTGGAG TTCTTCGCCC ACCCCGGGCT CGATCCCCTC GCGAGTTGGT
  661 TCAGCTGCTG CCTGANGCTG GACGACCTCG CGGAGTTCTA CCGGCAGTGC AAATCCGTCN
  721 GCATCCAGGA AACCAGCAGC GGCTATCCGC GCATCCATGC CCCCGAACTG CANGAGTGGN
  781 GAGGCACGAT GGCCGCTTTG GNCCGGATCT TTGTGAAGGA NCCTTACTTC TGTGGTGTGA
  841 CATANTTGGA CAAACTACCT ACNGANNNTT AAAGCTCTNA GGNAAATANA AAATTTTNAA
  901 GTGNATNATG TGTTAAACTA CNGATTCTNN TTGNTTGTGT ATTTTANANT NNNNCNNANN
  961 NNNNTGNNGN ANGGNNNCNG NNNNNGNNNN NNNNTNNNNN NNNAAANNNN NNTNNNNNNN
1021 NNN
//
Primer: Neo-B_R (Pr0911)
Region: NeoR_KanR,SV40 pro_ori
Sequence file: RDB03952_A9Fad.seq check
>03952_03952_B3B1F_1_Neo-B_R_F01_16_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNTGCGT GCNATCNNTC TTGTTCNATC ATGCGAAACG
   61 ATCCTCATCC TGTCTCTTGA TCAGATCTTG ATCCCCTGCG CCATCAGATC CTTGGCGGCA
  121 AGAAAGCCAT CCAGTTTACT TTGCAGGGCT TCCCAACCTT ACCAGAGGGC GCCCCAGCTG
  181 GCAATTCCGG TTCGCTTGCT GTCCATAAAA CCGCCCAGTC TAGCTATCGC CATGTAAGCC
  241 CACTGCAAGC TACCTGCTTT CTCTTTGCGC TTGCGTTTTC CCTTGTCCAG ATAGCCCAGT
  301 AGCTGACATT CATCCGGGGT CAGCACCGTT TCTGCGGACT GGCTTTCTAC GTGTTCCGCT
  361 TCCTTTAGCA GCCCTTGCGC CCTGAGTGCT TGCGGCAGCG TGAAGCTTTT TGCAAAAGCC
  421 TAGGCCTCCA AAAAAGCCTC CTCACTACTT CTGGAATAGC TCAGAGGCCG AGGCGGCCTC
  481 GGCCTCTGCA TAAATAAAAA AAATTAGTCA GCCATGGGGC GGAGAATGGG CGGAACTGGG
  541 CGGAGTTAGG GGCGGGATGG GCGGAGTTAG GGGCGGGACT ATGGTTGCTG ACTAATTGAG
  601 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCTGGTTGC
  661 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC
  721 ACACCCTAAC TGACACACAT TCCACAGCCG GATCTGCAGG ANCCAACGCT GCCCGAGANG
  781 CGCCGCGTGC GGCTGCTGGA NATGGCGGAN GCGATGGATA TGTTCTGCCN AGGGTTGGTT
  841 TGCGCATTCA CAGTTCTCCG CAAGAANTGA TTGGCTNCNA TTCTTGGANT GGTGAATNCG
  901 TTANCGAGGN GCCGCCNGCT TCCNTNCNNN CGAGGNGGNC CGGCNNCNNN NNNNNNGNNG
961 CNNNNCGGGG NNNCNNANNN NNNNNANGGN NGNNNNNTAN NNNNNNNNN
//
Primer: AxIT_R (Pr0347)
Region: ITR,cos 3'
Sequence file: RDB03952_A9Fae.seq check
>03952_03952_A9Fa_1_AxIT_R_F05_17_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NAAGCCNNAA AACCCACAAC TTCCTCAAAT CGTCACTTCC
   61 GTTTTCCCAC GTTACGTCAC TTCCCNTTTT AAGAAAACTA CAATTCCCAA CACATACAAG
  121 TTACTCCGCC CTAAAACCTA CGTCACCCGC CCCGTTCCCA CGCCCCGCGC CACGTCACAA
  181 ACTCCACCCC CTCATTATCA TATTGGCTTC AATCCAAAAT AAGGTATATT ATTGATGATG
  241 AAGCTTGGTT AATTAACGTT AATTAACCGG CGGCCGCCAC CGCTTCGATA AGCTTTAATG
  301 CGGTAGTTTA TCACAGTTAA ATTGCTAACG CAGTCAGGCA CCGTGTATGA AATCTAACAA
  361 TGCGCTCATC GTCATCCTCG GCACCGTCAC CCTGGATGCT GTAGGCATAG GCTTGGTTAT
  421 GCCGGTACTG CCGGGCCTCT TGCGGGATAT CGTCCATTCC GACAGCATCG CCAGTCACTA
  481 TGGCGTGCTG CTAGCGCTAT ATGCGTTGAT GCAATTTCTA TGCGCACCCG TTCTCGGAGC
  541 ACTGTCCGAC CGCTTTGGCC GCCGCCCAGT CCTGCTCGCT TCGCTACTTG GAGCCACTAT
  601 CGACTACGCG ATCATGGCGA CCACACCCGT CCTGTGGATC TGCCTCGCTG GCCTGCCGCA
  661 GTTCTTCAAC CTCCCGGCGC AGCTTTTCGT TCTCAATTTC AGCATCCCTT TCGGCATACC
  721 ATTTTATGAC GGCGGCAGAG TCATAAAGCA CCTCATTACC CTTGCCACCG CCTCGCAGAA
  781 CGGGCATTCC CTGTTCCTGC CAGTTCTGAA TGGTANGGAT ACTCGCACCG AAAATGTCAG
  841 CCAGCTGCTT TTTGTTGANT TCCATTGTNC ANTCCACGGN NNAAAACAGA GAAAGGAANN
  901 GACAGANGCC AAAAAGCTCG CTTTCAGCAC CNGTCGTTTC CTTTCNTTTN ANANGGNATT
961 TTNAATNAAA ACNTTAANTT ATGANGANNN NNNNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Tsuda, H., Efficient BMP2 gene transfer and bone formation of mesenchymal stem cells by a fiber-mutant adenoviral vector. Mol. Ther., 7, 354-365 (2003). PMID 12668131.
original Nakamura, T., Effective gene transfer to human melanomas via integrin-targeted adenoviral vectors. Hum. Gene Ther., 13, 613-626 (2002). PMID 11916485.